FvH4_5g08283

Purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001351
Physical Location & Seq
Reverse (-)
4817916 .. 4821851
3936 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g08283.t1

Sequence Viewer

Length: 1932 bp
ATGAGTGTTAGAGAATACTGCTCTATAAAATGGTTTGATCATGTCTGGATCACATCAGTCACACACATTATGGGGAGCTTCCGCTTCTTCTTGTCCCTTCAAGTTCTGCTGTTATCAGTTTTCTTACTGAATCCGACAGCAGCTCATAGTCACACTAAGTATGGAGGTGATGGTGTACAGCCACTGTCCAAGATTCAAATCCACAGAGCTGTTTATGAGCTTCACGAAAATGCTTCCGTCACAGCAAACCCAGTCCTCCTAGGTACCAAGGGGGGAGATTCTCAATGGGTAACTGTGGAAGTTGGCACTCCTAAGCCTTCTGATGATGATTGGTTGGCAGTCTTTTCTCCTGCAAATTTTAACTCATCGACTTGTACACCTAATTATGATGACATCTATGCTCCATGTATAGGCACTGCTCCTATAAAGTACAGGTTTGCCAATGAATCCAATTCTGCATATGCAAAGACAGGAAAAGCATCTTTGAAATTTCTGTTGATCAATCAAAGGGAAGATTTTTCGTTTGCGTTATTCTCCGGTGGGCTGTCAAATCCAAAAGTGGTAGCCGTTTCTAATGTTATATCTTTTGCAAATCCGAAGGCACCCCTTTACCCACGTCTTGCTCTAGGGAAGCACTGGGATGAAATGACGGTGACTTGGACAAGCGGCTATAATATTGATGAAGCTGTTCCATTTGTTGAATGGGGTATTAAGGGAGAGTCTCAAACTCGCTCTCCAGCAGGAACATTAACTTTTACTCGAGAGAGCATGTGCGGTCCACCAGCCCGGACAGTTGGTTGGCGCGATCCTGGTTTCATCCACACAAGTTTTCTGAAAGATTTGTGGCCAAATTCTTTGTACACTTACAAGCTTGGTCATAGGTTATCTAATAGTTCTTATATCTGGAGCAAGTCCTACTCTTTCAAGTCATCACCTTATCCCGGGCAGGACTCATTGCAGCATGTTGTAATATTCGGTGACATGGGAAAGGCTGAGCAGGATCGCTCAAACGAATATGCTGATTATCAGCCAGGATCCCTCATGACCAGGGATCAACTAGTCAAGGATTTGGATGAAATTGACATAGTTTTTCACATTGGAGATATGGCTTATTCCAATGGCTACATATCGCAGTGGGATCAGTTCACGTCGCAGGTGGAGCCGATTGCATCCACTGTACCATACATGATTGCAAGTGGCAATCATGAAAGAGATGTACCAGGGACAGGATCCTTCTACAACACTAATGATTCGGGTGGTGAATGTGGTGTGCTAGCCGAGACAATGTTCTATGTTCCTGCTGAGAACAGAGCTAAGTTCTGGTACTCGGCAGATTATGGAATGTTCCACTTCTGCATAGCAGACAGTGAGCATGACTGGAGAGAAGGTTCCGAGCAGTACAAATTCATCGAAAAATGCCTTGCATCAGCTGACAGGCAGAAACAGCCTTGGCTGATTTTCGCAGCTCATCGTGTTCTTGGTTATTCCTCTTCTTATTGGAATGATGGTTCTTTTGGAGAACCAATGGCAAGGGAAAACTTGCAAAAGCTTTGGCAGAAGTACAAGGTTGACATTGCATTTTTCGGCCATGTCCACAACTACGAAAGAACATGCCCCATTTATCAGAACCAGTGTGTCAACTCAGAAACATCTCATTATTCCGGAACTGTGAATGGAACGATCCATGTTGTTGTTGGTGGAGGAGGCAGCCACTTAGCGGGTTTTGGACCAGTGCAGACCAGTTGGAGCCTTTTCAAGGATTCCGATTTCGGCTTCACAAAGCTCACAGCATTTAACCACACATCGCTTCTCTTGGAGTACAAGAAAAGCAGTGATGGGAAGGTCTATGATTCCTTCACCATTTCAAGGAACTACAGGGATGTCTTGGCTTGTGTACATGATGGCTGTGAACCAACTACTCTAGCTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

644

Amino Acids

72.03

Weight (kDa)

5.93

Isoelectric Point (pI)

32.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_PAP PF17808 80 - 198 7.4e-43 Fn3-like domain from Purple Acid Phosphatase
Pur_ac_phosph_N PF16656 205 - 309 4.8e-13 Purple acid Phosphatase, N-terminal domain
Metallophos PF00149 322 - 534 2.8e-15 Calcineurin-like phosphoesterase
Metallophos_C PF14008 559 - 618 5.1e-17 Iron/zinc purple acid phosphatase-like protein C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1144
Acc36I ACCTGC 1 cut(s) 1144
Acc65I GGTACC 1 cut(s) 263
AccB1I GGYRCC 2 cut(s) 263, 601
AccII CGCG 1 cut(s) 804
AccIII TCCGGA 1 cut(s) 1661
AciI CCGC 4 cut(s) 82, 666, 774, 1718
AcoI YGGCCR 2 cut(s) 845, 1585
AcsI RAATTY 4 cut(s) 355, 488, 850, 1403
AfiI CCNNNNNNNGG 6 cut(s) 410, 941, 1226, 1717, 1756, 1866
AgsI TTSAA 7 cut(s) 101, 197, 487, 701, 925, 1756, 1866
AhlI ACTAGT 1 cut(s) 1057
AjiI CACGTC 2 cut(s) 617, 1149
AjnI CCWGG 4 cut(s) 808, 1030, 1046, 1219
AloI GAACNNNNNNTCC 4 cut(s) 1372, 1404, 1492, 1524
Alw26I GTCTC 2 cut(s) 726, 1274
AlwNI CAGNNNCTG 1 cut(s) 184
Ama87I CYCGRG 2 cut(s) 759, 941
Aor13HI TCCGGA 1 cut(s) 1661
AoxI GGCC 2 cut(s) 845, 1585
ApeKI GCWGC 4 cut(s) 140, 958, 1463, 1707
ApoI RAATTY 4 cut(s) 355, 488, 850, 1403
Asp700I GAANNNNTTC 1 cut(s) 687
Asp718I GGTACC 1 cut(s) 263
AspA2I CCTAGG 1 cut(s) 259
AspLEI GCGC 1 cut(s) 804
AspS9I GGNCC 2 cut(s) 776, 1727
AsuC2I CCSGG 3 cut(s) 787, 942, 943
AsuHPI GGTGA 6 cut(s) 179, 664, 924, 989, 1271, 1849
AsuNHI GCTAGC 1 cut(s) 1273
AvaI CYCGRG 2 cut(s) 759, 941
AvaII GGWCC 2 cut(s) 776, 1727
AvrII CCTAGG 1 cut(s) 259
BalI TGGCCA 1 cut(s) 847
BamHI GGATCC 2 cut(s) 1034, 1229
BanI GGYRCC 2 cut(s) 263, 601
BbvI GCAGC 4 cut(s) 152, 970, 1475, 1719
BccI CCATC 4 cut(s) 164, 1499, 1829, 1895
BceAI ACGGC 1 cut(s) 551
BciT130I CCWGG 4 cut(s) 810, 1032, 1048, 1221
BclI TGATCA 2 cut(s) 37, 498
BcnI CCSGG 3 cut(s) 787, 942, 943
BcoDI GTCTC 2 cut(s) 726, 1274
BcuI ACTAGT 1 cut(s) 1057
BfaI CTAG 5 cut(s) 260, 626, 1058, 1274, 1922
BfmI CTRYAG 1 cut(s) 1873
BfuAI ACCTGC 1 cut(s) 1144
BisI GCNGC 5 cut(s) 141, 667, 959, 1464, 1708
BlnI CCTAGG 1 cut(s) 259
BlpI GCTNAGC 1 cut(s) 993
BlsI GCNGC 5 cut(s) 142, 668, 960, 1465, 1709
Bme1390I CCNGG 7 cut(s) 787, 810, 942, 943, 1032, 1048, 1221
Bme18I GGWCC 2 cut(s) 776, 1727
BmeT110I CYCGRG 2 cut(s) 759, 941
BmgBI CACGTC 2 cut(s) 617, 1149
BmgT120I GGNCC 2 cut(s) 776, 1727
BmiI GGNNCC 7 cut(s) 265, 603, 1036, 1161, 1231, 1390, 1748
BmrFI CCNGG 7 cut(s) 787, 810, 942, 943, 1032, 1048, 1221
BmrI ACTGGG 2 cut(s) 245, 646
BmsI GCATC 3 cut(s) 488, 1178, 1433
BmtI GCTAGC 1 cut(s) 1277
BmuI ACTGGG 2 cut(s) 245, 646
BpmI CTGGAG 3 cut(s) 720, 925, 1399
Bpu10I CCTNAGC 1 cut(s) 312
Bpu1102I GCTNAGC 1 cut(s) 993
BpuMI CCSGG 3 cut(s) 787, 942, 943
BsaBI GATNNNNATC 1 cut(s) 197
BsaJI CCNNGG 6 cut(s) 259, 267, 941, 1047, 1220, 1448
BsaWI WCCGGW 2 cut(s) 536, 1661
BsaXI ACNNNNNCTCC 4 cut(s) 718, 748, 1372, 1402
Bsc4I CCNNNNNNNGG 6 cut(s) 410, 941, 1226, 1717, 1756, 1866
Bse1I ACTGG 6 cut(s) 251, 641, 1382, 1630, 1730, 1740
Bse3DI GCAATG 2 cut(s) 953, 1572
Bse8I GATNNNNATC 1 cut(s) 197
BseAI TCCGGA 1 cut(s) 1661
BseBI CCWGG 4 cut(s) 810, 1032, 1048, 1221
BseDI CCNNGG 6 cut(s) 259, 267, 941, 1047, 1220, 1448
BseGI GGATG 5 cut(s) 646, 816, 1078, 1169, 1885
BseJI GATNNNNATC 1 cut(s) 197
BseLI CCNNNNNNNGG 6 cut(s) 410, 941, 1226, 1717, 1756, 1866
BseMI GCAATG 2 cut(s) 953, 1572
BseMII CTCAG 3 cut(s) 984, 1293, 1656
BseNI ACTGG 6 cut(s) 251, 641, 1382, 1630, 1730, 1740
BseRI GAGGAG 1 cut(s) 1716
BseXI GCAGC 4 cut(s) 152, 970, 1475, 1719
BsgI GTGCAG 1 cut(s) 1754
Bsh1236I CGCG 1 cut(s) 804
BshFI GGCC 2 cut(s) 847, 1587
BshNI GGYRCC 2 cut(s) 263, 601
BsiHKCI CYCGRG 2 cut(s) 759, 941
BsiSI CCGG 4 cut(s) 537, 787, 942, 1662
BslFI GGGAC 2 cut(s) 79, 1237
BslI CCNNNNNNNGG 6 cut(s) 410, 941, 1226, 1717, 1756, 1866
BsmAI GTCTC 2 cut(s) 726, 1274
BsmFI GGGAC 2 cut(s) 79, 1237
BsnI GGCC 2 cut(s) 847, 1587
BsoBI CYCGRG 2 cut(s) 759, 941
Bsp13I TCCGGA 1 cut(s) 1661
Bsp1407I TGTACA 4 cut(s) 175, 374, 858, 1894
Bsp1720I GCTNAGC 1 cut(s) 993
BspACI CCGC 4 cut(s) 82, 666, 774, 1718
BspANI GGCC 2 cut(s) 847, 1587
BspCNI CTCAG 3 cut(s) 985, 1294, 1655
BspEI TCCGGA 1 cut(s) 1661
BspFNI CGCG 1 cut(s) 804
BspHI TCATGA 2 cut(s) 1041, 1204
BspLI GGNNCC 7 cut(s) 265, 603, 1036, 1161, 1231, 1390, 1748
BspMI ACCTGC 1 cut(s) 1144
BspOI GCTAGC 1 cut(s) 1277
BspT107I GGYRCC 2 cut(s) 263, 601
BsrDI GCAATG 2 cut(s) 953, 1572
BsrGI TGTACA 4 cut(s) 175, 374, 858, 1894
BsrI ACTGG 6 cut(s) 251, 641, 1382, 1630, 1730, 1740
BssECI CCNNGG 6 cut(s) 259, 267, 941, 1047, 1220, 1448
BssT1I CCWWGG 3 cut(s) 259, 267, 1448
Bst2UI CCWGG 4 cut(s) 810, 1032, 1048, 1221
Bst4CI ACNGT 7 cut(s) 186, 295, 652, 793, 1177, 1367, 1669
Bst6I CTCTTC 1 cut(s) 1495
BstAUI TGTACA 4 cut(s) 175, 374, 858, 1894
BstC8I GCNNGC 1 cut(s) 1275
BstDEI CTNAG 7 cut(s) 156, 312, 993, 1302, 1314, 1642, 1714
BstENI CCTNNNNNAGG 1 cut(s) 1754
BstF5I GGATG 5 cut(s) 646, 816, 1078, 1169, 1885
BstFNI CGCG 1 cut(s) 804
BstHHI GCGC 1 cut(s) 804
BstMAI GTCTC 2 cut(s) 726, 1274
BstMWI GCNNNNNNNGC 2 cut(s) 1159, 1444
BstNI CCWGG 4 cut(s) 810, 1032, 1048, 1221
BstNSI RCATGY 3 cut(s) 772, 965, 1614
BstSCI CCNGG 7 cut(s) 785, 808, 940, 941, 1030, 1046, 1219
BstSFI CTRYAG 1 cut(s) 1873
BstUI CGCG 1 cut(s) 804
BstV1I GCAGC 4 cut(s) 152, 970, 1475, 1719
BstX2I RGATCY 2 cut(s) 1034, 1229
BstYI RGATCY 2 cut(s) 1034, 1229
BsuRI GGCC 2 cut(s) 847, 1587
BtgZI GCGATG 1 cut(s) 1788
BtrI CACGTC 2 cut(s) 617, 1149
BtsCI GGATG 5 cut(s) 646, 816, 1078, 1169, 1885
BtsI GCAGTG 3 cut(s) 414, 1139, 1837
BtsIMutI CAGTG 9 cut(s) 182, 414, 634, 1139, 1173, 1372, 1637, 1737, 1837
BveI ACCTGC 1 cut(s) 1144
Cac8I GCNNGC 1 cut(s) 1275
CaiI CAGNNNCTG 1 cut(s) 184
CciI TCATGA 2 cut(s) 1041, 1204
CfoI GCGC 1 cut(s) 804
Cfr13I GGNCC 2 cut(s) 776, 1727
Cfr9I CCCGGG 1 cut(s) 941
DdeI CTNAG 7 cut(s) 156, 312, 993, 1302, 1314, 1642, 1714
EaeI YGGCCR 2 cut(s) 845, 1585
Eam1104I CTCTTC 1 cut(s) 1495
EarI CTCTTC 1 cut(s) 1495
Eco130I CCWWGG 3 cut(s) 259, 267, 1448
Eco47I GGWCC 2 cut(s) 776, 1727
Eco88I CYCGRG 2 cut(s) 759, 941
EcoNI CCTNNNNNAGG 1 cut(s) 1754
EcoRII CCWGG 4 cut(s) 808, 1030, 1046, 1219
EcoT14I CCWWGG 3 cut(s) 259, 267, 1448
ErhI CCWWGG 3 cut(s) 259, 267, 1448
FaqI GGGAC 2 cut(s) 79, 1237
FauI CCCGC 1 cut(s) 1711
FauNDI CATATG 1 cut(s) 460
FbaI TGATCA 2 cut(s) 37, 498
Fnu4HI GCNGC 5 cut(s) 141, 667, 959, 1464, 1708
FokI GGATG 5 cut(s) 653, 803, 1085, 1156, 1892
Fsp4HI GCNGC 5 cut(s) 141, 667, 959, 1464, 1708
FspBI CTAG 5 cut(s) 260, 626, 1058, 1274, 1922
GlaI GCGC 1 cut(s) 803
GluI GCNGC 5 cut(s) 141, 667, 959, 1464, 1708
GsuI CTGGAG 3 cut(s) 720, 925, 1399
HaeIII GGCC 2 cut(s) 847, 1587
HapII CCGG 4 cut(s) 537, 787, 942, 1662
HhaI GCGC 1 cut(s) 804
Hin6I GCGC 1 cut(s) 802
HinP1I GCGC 1 cut(s) 802
HincII GTYRAC 2 cut(s) 1570, 1639
HindII GTYRAC 2 cut(s) 1570, 1639
HindIII AAGCTT 2 cut(s) 869, 1547
HinfI GANTC 9 cut(s) 130, 193, 278, 446, 719, 950, 1250, 1760, 1850
HpaII CCGG 4 cut(s) 537, 787, 942, 1662
HphI GGTGA 6 cut(s) 179, 664, 924, 989, 1271, 1849
Hpy188I TCNGA 8 cut(s) 135, 322, 597, 834, 1393, 1626, 1645, 1765
Hpy188III TCNNGA 7 cut(s) 46, 224, 761, 904, 1042, 1205, 1662
Hpy99I CGWCG 1 cut(s) 1153
HpyAV CCTTC 7 cut(s) 107, 327, 592, 1243, 1379, 1834, 1864
HpyCH4III ACNGT 7 cut(s) 186, 295, 652, 793, 1177, 1367, 1669
HpyCH4IV ACGT 2 cut(s) 616, 1148
HpyF10VI GCNNNNNNNGC 2 cut(s) 1159, 1444
HpyF3I CTNAG 7 cut(s) 156, 312, 993, 1302, 1314, 1642, 1714
HpySE526I ACGT 2 cut(s) 616, 1148
HspAI GCGC 1 cut(s) 802
Kpn2I TCCGGA 1 cut(s) 1661
KpnI GGTACC 1 cut(s) 267
Ksp22I TGATCA 2 cut(s) 37, 498
LmnI GCTCC 6 cut(s) 75, 406, 424, 906, 1159, 1746
Lsp1109I GCAGC 4 cut(s) 152, 970, 1475, 1719
LweI GCATC 3 cut(s) 488, 1178, 1433
MaeI CTAG 5 cut(s) 260, 626, 1058, 1274, 1922
MaeII ACGT 2 cut(s) 616, 1148
MaeIII GTNAC 6 cut(s) 58, 149, 238, 289, 652, 977
MboII GAAGA 3 cut(s) 79, 524, 1482
MflI RGATCY 2 cut(s) 1034, 1229
MlsI TGGCCA 1 cut(s) 847
MluCI AATT 7 cut(s) 355, 382, 451, 488, 850, 1077, 1403
MluNI TGGCCA 1 cut(s) 847
MlyI GAGTC 2 cut(s) 728, 944
MmeI TCCRAC 2 cut(s) 158, 1724
MnlI CCTC 6 cut(s) 158, 266, 1049, 1498, 1694, 1697
Mox20I TGGCCA 1 cut(s) 847
MroI TCCGGA 1 cut(s) 1661
MroXI GAANNNNTTC 1 cut(s) 687
MscI TGGCCA 1 cut(s) 847
MseI TTAA 4 cut(s) 360, 711, 749, 1794
MslI CAYNNNNRTG 2 cut(s) 228, 639
Msp20I TGGCCA 1 cut(s) 847
MspA1I CMGCKG 1 cut(s) 1430
MspI CCGG 4 cut(s) 537, 787, 942, 1662
MspR9I CCNGG 7 cut(s) 787, 810, 942, 943, 1032, 1048, 1221
MvaI CCWGG 4 cut(s) 810, 1032, 1048, 1221
MvnI CGCG 1 cut(s) 804
MwoI GCNNNNNNNGC 2 cut(s) 1159, 1444
NciI CCSGG 3 cut(s) 787, 942, 943
NdeI CATATG 1 cut(s) 460
NheI GCTAGC 1 cut(s) 1273
NlaIV GGNNCC 7 cut(s) 265, 603, 1036, 1161, 1231, 1390, 1748
NmeAIII GCCGAG 2 cut(s) 1303, 1307
NmuCI GTSAC 5 cut(s) 58, 149, 238, 652, 977
NspI RCATGY 3 cut(s) 772, 965, 1614
PaeR7I CTCGAG 1 cut(s) 759
PagI TCATGA 2 cut(s) 1041, 1204
PaqCI CACCTGC 1 cut(s) 1144
PdmI GAANNNNTTC 1 cut(s) 687
PfeI GAWTC 7 cut(s) 130, 193, 278, 446, 1250, 1760, 1850
PkrI GCNGC 5 cut(s) 142, 668, 960, 1465, 1709
PleI GAGTC 2 cut(s) 727, 944
PpsI GAGTC 2 cut(s) 727, 944
Psp6I CCWGG 4 cut(s) 808, 1030, 1046, 1219
PspGI CCWGG 4 cut(s) 808, 1030, 1046, 1219
PspN4I GGNNCC 7 cut(s) 265, 603, 1036, 1161, 1231, 1390, 1748
PspPI GGNCC 2 cut(s) 776, 1727
PstNI CAGNNNCTG 1 cut(s) 184
PsuI RGATCY 2 cut(s) 1034, 1229
PvuII CAGCTG 1 cut(s) 1430
RseI CAYNNNNRTG 2 cut(s) 228, 639
SaqAI TTAA 4 cut(s) 360, 711, 749, 1794
SatI GCNGC 5 cut(s) 141, 667, 959, 1464, 1708
Sau96I GGNCC 2 cut(s) 776, 1727
SchI GAGTC 2 cut(s) 728, 944
ScrFI CCNGG 7 cut(s) 787, 810, 942, 943, 1032, 1048, 1221
SfaNI GCATC 3 cut(s) 488, 1178, 1433
SfcI CTRYAG 1 cut(s) 1873
Sfr274I CTCGAG 1 cut(s) 759
SinI GGWCC 2 cut(s) 776, 1727
SlaI CTCGAG 1 cut(s) 759
SmaI CCCGGG 1 cut(s) 943
SmiMI CAYNNNNRTG 2 cut(s) 228, 639
SmlI CTYRAG 1 cut(s) 759
SmoI CTYRAG 1 cut(s) 759
SpeI ACTAGT 1 cut(s) 1057
Sse9I AATT 7 cut(s) 355, 382, 451, 488, 850, 1077, 1403
SsiI CCGC 4 cut(s) 82, 666, 774, 1718
SspI AATATT 2 cut(s) 676, 972
SspMI CTAG 5 cut(s) 260, 626, 1058, 1274, 1922
StyD4I CCNGG 7 cut(s) 785, 808, 940, 941, 1030, 1046, 1219
StyI CCWWGG 3 cut(s) 259, 267, 1448
TaaI ACNGT 7 cut(s) 186, 295, 652, 793, 1177, 1367, 1669
TaiI ACGT 2 cut(s) 619, 1151
TaqI TCGA 3 cut(s) 368, 760, 1410
TasI AATT 7 cut(s) 355, 382, 451, 488, 850, 1077, 1403
TatI WGTACW 8 cut(s) 175, 374, 429, 858, 1398, 1560, 1818, 1894
TauI GCSGC 1 cut(s) 669
TfiI GAWTC 7 cut(s) 130, 193, 278, 446, 1250, 1760, 1850
Tru1I TTAA 4 cut(s) 360, 711, 749, 1794
Tru9I TTAA 4 cut(s) 360, 711, 749, 1794
TscAI CASTG 9 cut(s) 189, 421, 641, 1139, 1180, 1372, 1637, 1737, 1837
TseFI GTSAC 5 cut(s) 58, 149, 238, 652, 977
TseI GCWGC 4 cut(s) 140, 958, 1463, 1707
Tsp45I GTSAC 5 cut(s) 58, 149, 238, 652, 977
TspDTI ATGAA 7 cut(s) 459, 657, 696, 805, 1089, 1221, 1396
TspGWI ACGGA 1 cut(s) 226
TspMI CCCGGG 1 cut(s) 941
TspRI CASTG 9 cut(s) 189, 421, 641, 1139, 1180, 1372, 1637, 1737, 1837
VpaK11BI GGWCC 2 cut(s) 776, 1727
XagI CCTNNNNNAGG 1 cut(s) 1754
XapI RAATTY 4 cut(s) 355, 488, 850, 1403
XceI RCATGY 3 cut(s) 772, 965, 1614
XcmI CCANNNNNNNNNTGG 2 cut(s) 699, 1691
XhoI CTCGAG 1 cut(s) 759
XmaI CCCGGG 1 cut(s) 941
XmaJI CCTAGG 1 cut(s) 259
XmnI GAANNNNTTC 1 cut(s) 687
XspI CTAG 5 cut(s) 260, 626, 1058, 1274, 1922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.