AT5G54620

ankyrin repeat family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003301
Physical Location & Seq
Reverse (-)
22187663 .. 22189746
2084 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54620.1

Sequence Viewer

Length: 1296 bp
ATGGATCGAAGGTTACTTTGGGTTACTGATTCGGGTAACGTTGATGCTTTATATGCTCTAATTCACAAGGATCCATATATTTTACAAAACATTGATGTTTTACCTTTCGTCCACACACCTCTCCATGAAGCTTCATCAACCGGAAAGACAGATTTGGCGATGGAACTGATGGTTTTAAAACCAACTTTTGCCAAAAAACTAAACTCGGATGGGGTTAGTCCTTTGCATCTCGCTGTCGAAAACCACCAAGTTCAGTTAGCACTAGAACTAGTCAAGATTAATCCTGATCTCGTTCTTGTTGCTGGGAGAAAAGGCATGACACCACTACATCTTGTAGTAAAAAAGGGCGATGCCAACCTTCTTACTGAGTTCTTACTGGCTTGTCCTGAGAGCATCAAAGACACGAACGTGAATGGCGAGACTGCTCTACATATTGCGGTGATGAATGATAGATACGAAGAGCTCAAAGTGCTCACAGGATGGATACATAGATTGCACAAAAGCGATGCTGCATCTACTGAGATTCATGTCTTGAACAAACGTGATCGTGATGGCAACACGATCTTGCATCTTGCGGCTTATAAGAATAATCATAAGGTAGTCAAAGAACTATTAAAATGCATATCGCTGAACCGGGACATCCAAAACAAAGGTGGTATGACAGCCCTTGATATTTTACGTACTAATGGATCTCACATGAACATAAAAACAGAGAAAATAATTCGACACTCAGGGGGTAAGAGCGGGGTTTCTCTATCAAAAGTTAAGACCGCATCTGTCTTCTTGAGATCGCCCATAACTTTTGTAGAATATTGCTCCACGACAATGACTCGCTATAAGAACCGCATGTCCGACGGAACACGAAATGCCCTTCTAGTAATAACAGCTCTGATTATCACAGCTACATATCAAACTGCCGTCCAACCGCAAGATAAAGATGAAATATATTACACGGGAAATATCATGATAAATGTGTTGTTTGTGTGGGGATTCAATACTATAGCATTTTGCTTGGCTATTGCCTTGACATTTATACTCTTACCTGTTGGTAAAGCATATAATTGGTGGTACATCTTTATTTCGGTGCCTCTCGTTTGTTCTTACGCACTTTCCATGTTTCTCAAGTATATACAATTCCCGATATTATGTATGCCTTTATACTTGATGATGGCATTTATACTCGGATTTCTAATCTATGTATTTGTACTATACGTGGGATGGAAGAGGGCTAAGCAGGAGAAGGTACCAGAACCAAAAAGTGAGCTGACTTTTGAAGGCTTCACGTCTATGACCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001508 GO:0002027 GO:0002376 GO:0003008 GO:0003012 GO:0003013 GO:0003015 GO:0003205 GO:0003230 GO:0003279 GO:0003283 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006888 GO:0006928 GO:0006936 GO:0006937 GO:0006941 GO:0006942 GO:0006950 GO:0006952 GO:0006955 GO:0007009 GO:0007154 GO:0007165 GO:0007267 GO:0007275 GO:0007507 GO:0008015 GO:0008016 GO:0008092 GO:0008104 GO:0008150 GO:0009719 GO:0009725 GO:0009751 GO:0009893 GO:0009987 GO:0010033 GO:0010256 GO:0010468 GO:0010522 GO:0010604 GO:0010628 GO:0010646 GO:0010880 GO:0010881 GO:0010882 GO:0010959 GO:0014070 GO:0014704 GO:0016020 GO:0016043 GO:0016192 GO:0016323 GO:0019222 GO:0019722 GO:0019725 GO:0019899 GO:0019900 GO:0019901 GO:0019932 GO:0022898 GO:0023051 GO:0023052 GO:0030001 GO:0030003 GO:0030016 GO:0030017 GO:0030018 GO:0030029 GO:0030048 GO:0030054 GO:0030154 GO:0030315 GO:0030507 GO:0030674 GO:0031430 GO:0031647 GO:0031672 GO:0031674 GO:0032409 GO:0032411 GO:0032412 GO:0032414 GO:0032501 GO:0032502 GO:0032879 GO:0032970 GO:0033036 GO:0033292 GO:0033365 GO:0034394 GO:0034613 GO:0034762 GO:0034764 GO:0034765 GO:0034767 GO:0035556 GO:0035637 GO:0036309 GO:0036371 GO:0042221 GO:0042383 GO:0042391 GO:0042493 GO:0042592 GO:0042692 GO:0043034 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043266 GO:0043268 GO:0043269 GO:0043270 GO:0043292 GO:0044057 GO:0044093 GO:0044291 GO:0044325 GO:0044422 GO:0044424 GO:0044425 GO:0044444 GO:0044449 GO:0044459 GO:0044464 GO:0045087 GO:0046677 GO:0046907 GO:0048193 GO:0048468 GO:0048513 GO:0048518 GO:0048522 GO:0048731 GO:0048856 GO:0048869 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050821 GO:0050896 GO:0051049 GO:0051050 GO:0051117 GO:0051179 GO:0051234 GO:0051239 GO:0051270 GO:0051279 GO:0051282 GO:0051641 GO:0051649 GO:0051716 GO:0051899 GO:0051924 GO:0051928 GO:0055001 GO:0055065 GO:0055074 GO:0055080 GO:0055082 GO:0055117 GO:0060047 GO:0060048 GO:0060090 GO:0060255 GO:0060306 GO:0060307 GO:0060341 GO:0061024 GO:0061061 GO:0061337 GO:0065007 GO:0065008 GO:0065009 GO:0070252 GO:0070296 GO:0070727 GO:0070838 GO:0070972 GO:0071840 GO:0071944 GO:0072359 GO:0072503 GO:0072507 GO:0072511 GO:0072657 GO:0072659 GO:0086001 GO:0086002 GO:0086003 GO:0086004 GO:0086005 GO:0086010 GO:0086012 GO:0086014 GO:0086015 GO:0086018 GO:0086019 GO:0086026 GO:0086046 GO:0086065 GO:0086066 GO:0086070 GO:0086091 GO:0090257 GO:0098590 GO:0098771 GO:0098900 GO:0098901 GO:0098907 GO:0098910 GO:0099080 GO:0099081 GO:0099512 GO:0099623 GO:1901016 GO:1901018 GO:1901019 GO:1901021 GO:1901379 GO:1901381 GO:1901700 GO:1903115 GO:1903169 GO:1903522 GO:1903779 GO:1904062 GO:1904064 GO:1904427 GO:1990778 GO:2001257 GO:2001259
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

431

Amino Acids

48.58

Weight (kDa)

9.06

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 7 - 94 3e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 100 - 158 9e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 175 - 225 1.6e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
Acc65I GGTACC 1 cut(s) 1243
AccB1I GGYRCC 2 cut(s) 1084, 1243
AccBSI CCGCTC 1 cut(s) 744
AciI CCGC 6 cut(s) 437, 575, 744, 771, 844, 926
AclI AACGTT 1 cut(s) 39
AclWI GGATC 4 cut(s) 12, 65, 78, 697
AfaI GTAC 4 cut(s) 682, 1070, 1206, 1245
AgsI TTSAA 3 cut(s) 535, 994, 1274
AhlI ACTAGT 1 cut(s) 268
AjiI CACGTC 1 cut(s) 1284
AjuI GAANNNNNNNTTGG 1 cut(s) 33
AleI CACNNNNGTG 1 cut(s) 407
AloI GAACNNNNNNTCC 2 cut(s) 833, 865
AluBI AGCT 5 cut(s) 131, 463, 887, 902, 1264
AluI AGCT 5 cut(s) 131, 463, 887, 902, 1264
Alw21I GWGCWC 2 cut(s) 465, 474
Alw26I GTCTC 1 cut(s) 413
AlwI GGATC 4 cut(s) 12, 65, 78, 697
ApeKI GCWGC 1 cut(s) 509
AseI ATTAAT 1 cut(s) 279
Asp718I GGTACC 1 cut(s) 1243
AsuC2I CCSGG 1 cut(s) 635
AsuHPI GGTGA 1 cut(s) 451
BamHI GGATCC 1 cut(s) 70
BanI GGYRCC 2 cut(s) 1084, 1243
BanII GRGCYC 1 cut(s) 465
BbsI GAAGAC 1 cut(s) 772
Bbv12I GWGCWC 2 cut(s) 465, 474
BbvI GCAGC 1 cut(s) 496
BccI CCATC 7 cut(s) 154, 163, 203, 474, 545, 1162, 1212
BceAI ACGGC 1 cut(s) 902
BciVI GTATCC 1 cut(s) 477
BcnI CCSGG 1 cut(s) 635
BcoDI GTCTC 1 cut(s) 413
BcuI ACTAGT 1 cut(s) 268
BfaI CTAG 3 cut(s) 263, 269, 875
BfmI CTRYAG 1 cut(s) 999
BfuI GTATCC 1 cut(s) 477
BisI GCNGC 2 cut(s) 510, 576
BlpI GCTNAGC 1 cut(s) 1230
BlsI GCNGC 2 cut(s) 511, 577
Bme1390I CCNGG 1 cut(s) 635
BmgBI CACGTC 1 cut(s) 1284
BmiI GGNNCC 3 cut(s) 72, 1086, 1245
BmrFI CCNGG 1 cut(s) 635
BmsI GCATC 8 cut(s) 34, 235, 340, 402, 496, 521, 577, 782
BpiI GAAGAC 1 cut(s) 772
Bpu1102I GCTNAGC 1 cut(s) 1230
BpuEI CTTGAG 2 cut(s) 805, 1106
BpuMI CCSGG 1 cut(s) 635
BsaAI YACGTR 2 cut(s) 680, 1213
BsaWI WCCGGW 1 cut(s) 140
Bse1I ACTGG 1 cut(s) 381
BseGI GGATG 4 cut(s) 214, 485, 639, 1223
BseMII CTCAG 4 cut(s) 357, 378, 510, 744
BseNI ACTGG 1 cut(s) 381
BseXI GCAGC 1 cut(s) 496
BseYI CCCAGC 1 cut(s) 302
BshNI GGYRCC 2 cut(s) 1084, 1243
BsiHKAI GWGCWC 2 cut(s) 465, 474
BsiSI CCGG 2 cut(s) 141, 634
BslFI GGGAC 1 cut(s) 650
BsmAI GTCTC 1 cut(s) 413
BsmFI GGGAC 1 cut(s) 650
Bsp1286I GDGCHC 2 cut(s) 465, 474
Bsp143I GATC 7 cut(s) 4, 70, 286, 544, 561, 689, 788
Bsp1720I GCTNAGC 1 cut(s) 1230
BspACI CCGC 6 cut(s) 437, 575, 744, 771, 844, 926
BspCNI CTCAG 4 cut(s) 358, 379, 511, 743
BspHI TCATGA 1 cut(s) 963
BspLI GGNNCC 3 cut(s) 72, 1086, 1245
BspPI GGATC 4 cut(s) 12, 65, 78, 697
BspQI GCTCTTC 1 cut(s) 453
BspT107I GGYRCC 2 cut(s) 1084, 1243
BsrBI CCGCTC 1 cut(s) 744
BsrI ACTGG 1 cut(s) 381
BssMI GATC 7 cut(s) 4, 70, 286, 544, 561, 689, 788
Bst6I CTCTTC 2 cut(s) 453, 1217
BstBAI YACGTR 2 cut(s) 680, 1213
BstDEI CTNAG 5 cut(s) 366, 387, 519, 730, 1230
BstF5I GGATG 4 cut(s) 214, 485, 639, 1223
BstKTI GATC 7 cut(s) 7, 73, 289, 547, 564, 692, 791
BstMAI GTCTC 1 cut(s) 413
BstMBI GATC 7 cut(s) 4, 70, 286, 544, 561, 689, 788
BstMWI GCNNNNNNNGC 2 cut(s) 53, 469
BstNSI RCATGY 1 cut(s) 850
BstSCI CCNGG 1 cut(s) 633
BstSFI CTRYAG 1 cut(s) 999
BstSNI TACGTA 1 cut(s) 680
BstV1I GCAGC 1 cut(s) 496
BstV2I GAAGAC 1 cut(s) 772
BstX2I RGATCY 2 cut(s) 70, 689
BstYI RGATCY 2 cut(s) 70, 689
BsuI GTATCC 1 cut(s) 477
BtgZI GCGATG 3 cut(s) 173, 363, 519
BtrI CACGTC 1 cut(s) 1284
BtsCI GGATG 4 cut(s) 214, 485, 639, 1223
CciI TCATGA 1 cut(s) 963
Csp6I GTAC 4 cut(s) 681, 1069, 1205, 1244
CviAII CATG 7 cut(s) 125, 316, 527, 697, 847, 964, 1114
CviQI GTAC 4 cut(s) 681, 1069, 1205, 1244
DdeI CTNAG 5 cut(s) 366, 387, 519, 730, 1230
DpnI GATC 7 cut(s) 6, 72, 288, 546, 563, 691, 790
DpnII GATC 7 cut(s) 4, 70, 286, 544, 561, 689, 788
DraI TTTAAA 1 cut(s) 177
Eam1104I CTCTTC 2 cut(s) 453, 1217
EarI CTCTTC 2 cut(s) 453, 1217
Ecl136II GAGCTC 1 cut(s) 463
Eco105I TACGTA 1 cut(s) 680
Eco24I GRGCYC 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 463
EcoICRI GAGCTC 1 cut(s) 463
EcoT22I ATGCAT 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 465
FaeI CATG 7 cut(s) 128, 319, 530, 700, 850, 967, 1117
FaqI GGGAC 1 cut(s) 650
FatI CATG 7 cut(s) 124, 315, 526, 696, 846, 963, 1113
FauI CCCGC 1 cut(s) 737
Fnu4HI GCNGC 2 cut(s) 510, 576
FokI GGATG 4 cut(s) 221, 492, 626, 1230
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 2 cut(s) 510, 576
FspBI CTAG 3 cut(s) 263, 269, 875
GluI GCNGC 2 cut(s) 510, 576
GsaI CCCAGC 1 cut(s) 306
HapII CCGG 2 cut(s) 141, 634
Hin1II CATG 7 cut(s) 128, 319, 530, 700, 850, 967, 1117
HindIII AAGCTT 1 cut(s) 129
HinfI GANTC 4 cut(s) 29, 523, 829, 990
HpaII CCGG 2 cut(s) 141, 634
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 4 cut(s) 208, 853, 891, 1184
Hpy188III TCNNGA 8 cut(s) 274, 284, 386, 532, 548, 784, 964, 1138
Hpy8I GTNNAC 1 cut(s) 112
Hpy99I CGWCG 1 cut(s) 857
HpyAV CCTTC 5 cut(s) 3, 368, 881, 1234, 1268
HpyCH4IV ACGT 6 cut(s) 39, 408, 541, 679, 1212, 1283
HpyCH4V TGCA 5 cut(s) 226, 496, 512, 568, 621
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 469
HpyF3I CTNAG 5 cut(s) 366, 387, 519, 730, 1230
HpySE526I ACGT 6 cut(s) 39, 408, 541, 679, 1212, 1283
Hsp92II CATG 7 cut(s) 128, 319, 530, 700, 850, 967, 1117
KpnI GGTACC 1 cut(s) 1247
Kzo9I GATC 7 cut(s) 4, 70, 286, 544, 561, 689, 788
LguI GCTCTTC 1 cut(s) 453
LmnI GCTCC 1 cut(s) 821
Lsp1109I GCAGC 1 cut(s) 496
LweI GCATC 8 cut(s) 34, 235, 340, 402, 496, 521, 577, 782
MaeI CTAG 3 cut(s) 263, 269, 875
MaeII ACGT 6 cut(s) 39, 408, 541, 679, 1212, 1283
MaeIII GTNAC 3 cut(s) 12, 22, 35
MalI GATC 7 cut(s) 6, 72, 288, 546, 563, 691, 790
MbiI CCGCTC 1 cut(s) 744
MboI GATC 7 cut(s) 4, 70, 286, 544, 561, 689, 788
MboII GAAGA 3 cut(s) 470, 772, 1234
MflI RGATCY 2 cut(s) 70, 689
MhlI GDGCHC 2 cut(s) 465, 474
MluCI AATT 4 cut(s) 60, 720, 1060, 1133
MlyI GAGTC 1 cut(s) 823
MmeI TCCRAC 2 cut(s) 876, 946
MnlI CCTC 3 cut(s) 129, 1098, 1218
Mph1103I ATGCAT 1 cut(s) 623
MseI TTAA 4 cut(s) 176, 279, 614, 765
MslI CAYNNNNRTG 3 cut(s) 407, 824, 1286
MspI CCGG 2 cut(s) 141, 634
MspR9I CCNGG 1 cut(s) 635
MwoI GCNNNNNNNGC 2 cut(s) 53, 469
NciI CCSGG 1 cut(s) 635
NdeII GATC 7 cut(s) 4, 70, 286, 544, 561, 689, 788
NlaIII CATG 7 cut(s) 128, 319, 530, 700, 850, 967, 1117
NlaIV GGNNCC 3 cut(s) 72, 1086, 1245
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 1 cut(s) 850
OliI CACNNNNGTG 1 cut(s) 407
PagI TCATGA 1 cut(s) 963
PciSI GCTCTTC 1 cut(s) 453
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PfeI GAWTC 3 cut(s) 29, 523, 990
PkrI GCNGC 2 cut(s) 511, 577
PleI GAGTC 1 cut(s) 823
PpsI GAGTC 1 cut(s) 823
Ppu21I YACGTR 2 cut(s) 680, 1213
PshBI ATTAAT 1 cut(s) 279
PsiI TTATAA 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 465
Psp1406I AACGTT 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 302
PspN4I GGNNCC 3 cut(s) 72, 1086, 1245
PsuI RGATCY 2 cut(s) 70, 689
RsaI GTAC 4 cut(s) 682, 1070, 1206, 1245
RsaNI GTAC 4 cut(s) 681, 1069, 1205, 1244
RseI CAYNNNNRTG 3 cut(s) 407, 824, 1286
SacI GAGCTC 1 cut(s) 465
SapI GCTCTTC 1 cut(s) 453
SaqAI TTAA 4 cut(s) 176, 279, 614, 765
SatI GCNGC 2 cut(s) 510, 576
Sau3AI GATC 7 cut(s) 4, 70, 286, 544, 561, 689, 788
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 1 cut(s) 635
SduI GDGCHC 2 cut(s) 465, 474
SfaNI GCATC 8 cut(s) 34, 235, 340, 402, 496, 521, 577, 782
SfcI CTRYAG 1 cut(s) 999
SmiMI CAYNNNNRTG 3 cut(s) 407, 824, 1286
SmlI CTYRAG 2 cut(s) 784, 1121
SmoI CTYRAG 2 cut(s) 784, 1121
SnaBI TACGTA 1 cut(s) 680
SpeI ACTAGT 1 cut(s) 268
Sse9I AATT 4 cut(s) 60, 720, 1060, 1133
SsiI CCGC 6 cut(s) 437, 575, 744, 771, 844, 926
SspI AATATT 1 cut(s) 812
SspMI CTAG 3 cut(s) 263, 269, 875
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 1 cut(s) 633
TaiI ACGT 6 cut(s) 42, 411, 544, 682, 1215, 1286
TaqI TCGA 3 cut(s) 7, 237, 724
TasI AATT 4 cut(s) 60, 720, 1060, 1133
TatI WGTACW 1 cut(s) 1204
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 3 cut(s) 29, 523, 990
Tru1I TTAA 4 cut(s) 176, 279, 614, 765
Tru9I TTAA 4 cut(s) 176, 279, 614, 765
TseI GCWGC 1 cut(s) 509
TspDTI ATGAA 6 cut(s) 123, 141, 458, 515, 713, 954
TspGWI ACGGA 1 cut(s) 870
VspI ATTAAT 1 cut(s) 279
XceI RCATGY 1 cut(s) 850
XcmI CCANNNNNNNNNTGG 1 cut(s) 650
XspI CTAG 3 cut(s) 263, 269, 875
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.