Rh1BG350000

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0003301
Physical Location & Seq
Forward (+)
48309065 .. 48310831
1767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG350000.1

Sequence Viewer

Length: 1257 bp
ATGGATGAAAGGCTGATTGAGGCTTCTCGAAGAGGAAATGTCCAACTTTTGAATCAGTTGCTGGCAGAAAATCCAGACATACTCCATAGTGTTGCGCTGGCTTCGGGAGAGAACCCTTTACACATTGCTTCCTTGGCTGGCCATGTTGAGTTTGTTAAGGAGGTTCTAAGGTTGAAACCAGCTTTTGCTAAAGAAATCAACCAGGATTGTTATAGCCCGATGCATATTGCGTCTGCCAACGGGCATTTGGAGATTGTAAAGGAGCTGCTTCAAGTTGATCCAAGACTCTCCCAGCTTAAAGGAAGAGATCAATGTACAGCTCTTCATTATGCAGCTCGCAGAGGGAGGATAGAAATTATTAAAGAAATGGTTTCGGCTTGTTCAGAATGTGTCGAAGATGTGACCGAGCAAGGGGAGACTGTTTTTCACCTTGCTGTTAAGAACAACCAGTTTGAAGCTATTGATGCTATGGTGGAATTGGTGGTGCAATGTTTACTTGCCATTAACGGAACTAGTACTCCGGGTACATTGGACGTAAACAGTGTGAATCAGAGTGGTCTAACAGCTCTTGATCTGCTACTAATCTTCCCTAGAGGAGCCGGTGACCTGGAGATCGATGGTATCCTTCGCGGTGCAGGAGCTTTGAGAGCACGAGACATAGTCCACACTAATCACAACCATACCGTATTAAGTGTCCCCGCAGCATCAGAGAGCCATGATAGTCTGCTCATGGACCCAAAAAATTTGGTGGAGTACTTCAAGTTCAAAAGGGGCAGAGACTCTCCTGATGACGCACGAACCGCTCTGTTAGTCGTGGCAGTACTGGTGGCTACGGCAACCTATGACACTGCAATAAACCCTCCAGGTGGTCTTTGGCAGGACACAAACTTAAGCAAGAATGGGACTGATCCACCACACCTTGTCGGCACGGCGAATATCGCCTCTTATAACACAGCTTACTACGTCGTTGGGGCGGTTTTCAACTCCATAGGGTTTTCAGTTTCTCTTCACATGATCAATATCCTCACAACCAATTTCCCTTTGCGGTTGGAGCTTCAAGTTTGTGGTGTTGCAATGTACTTTACGTACGTCACTGCATTGATTACTATATCTCCAGGTTTGACCTCAGGGTGGTACACATTGTTTGTACTTGTGCCATTTCTGCCTATGGCGGTACTACTTGCCGTGAGGTTTATCATCAAGCTTACAGAACCTCTGCGAAATCGACTGCAGACCTGCGGATTTGTCCTAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

418

Amino Acids

45.63

Weight (kDa)

5.71

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 91 8.4e-13 Ankyrin repeats (3 copies)
Ank_2 PF12796 74 - 159 2.1e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 74 - 121 2.3e-06 Ankyrin repeats (many copies)
PGG PF13962 264 - 368 3.6e-14 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 948
Acc36I ACCTGC 1 cut(s) 1244
AccBSI CCGCTC 1 cut(s) 803
AccII CGCG 1 cut(s) 630
AciI CCGC 7 cut(s) 630, 699, 801, 974, 1045, 1172, 1239
AclWI GGATC 2 cut(s) 272, 902
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 742
AfiI CCNNNNNNNGG 3 cut(s) 411, 866, 1131
AflII CTTAAG 1 cut(s) 889
AgsI TTSAA 8 cut(s) 52, 175, 272, 455, 760, 766, 982, 1058
AhlI ACTAGT 1 cut(s) 512
AjnI CCWGG 4 cut(s) 201, 606, 862, 1114
AloI GAACNNNNNNTCC 2 cut(s) 502, 534
Alw21I GWGCWC 1 cut(s) 652
Alw26I GTCTC 3 cut(s) 410, 648, 771
AlwI GGATC 2 cut(s) 272, 902
AlwNI CAGNNNCTG 1 cut(s) 61
AoxI GGCC 1 cut(s) 139
ApeKI GCWGC 3 cut(s) 265, 332, 701
ApoI RAATTY 1 cut(s) 742
AspLEI GCGC 1 cut(s) 97
AspS9I GGNCC 1 cut(s) 733
AsuC2I CCSGG 1 cut(s) 522
AsuHPI GGTGA 2 cut(s) 419, 614
AvaII GGWCC 1 cut(s) 733
AxyI CCTNAGG 1 cut(s) 1126
BalI TGGCCA 1 cut(s) 141
BauI CACGAG 1 cut(s) 651
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 3 cut(s) 252, 344, 713
BccI CCATC 1 cut(s) 611
BceAI ACGGC 3 cut(s) 849, 945, 1169
BciT130I CCWGG 4 cut(s) 203, 608, 864, 1116
BciVI GTATCC 1 cut(s) 632
BclI TGATCA 1 cut(s) 1014
BcnI CCSGG 1 cut(s) 522
BcoDI GTCTC 3 cut(s) 410, 648, 771
BcuI ACTAGT 1 cut(s) 512
BfaI CTAG 2 cut(s) 513, 591
BfmI CTRYAG 1 cut(s) 1229
BfrI CTTAAG 1 cut(s) 889
BfuAI ACCTGC 1 cut(s) 1244
BfuI GTATCC 1 cut(s) 632
BisI GCNGC 3 cut(s) 266, 333, 702
BlsI GCNGC 3 cut(s) 267, 334, 703
BmcAI AGTACT 3 cut(s) 517, 755, 822
Bme1390I CCNGG 5 cut(s) 203, 522, 608, 864, 1116
Bme18I GGWCC 1 cut(s) 733
BmgT120I GGNCC 1 cut(s) 733
BmiI GGNNCC 2 cut(s) 598, 735
BmrFI CCNGG 5 cut(s) 203, 522, 608, 864, 1116
BmsI GCATC 3 cut(s) 210, 454, 713
BpmI CTGGAG 3 cut(s) 629, 846, 1098
BpuMI CCSGG 1 cut(s) 522
Bsa29I ATCGAT 1 cut(s) 615
BsaAI YACGTR 1 cut(s) 1086
BsaBI GATNNNNATC 1 cut(s) 1019
BsaJI CCNNGG 1 cut(s) 132
BsaXI ACNNNNNCTCC 4 cut(s) 502, 532, 1096, 1126
Bsc4I CCNNNNNNNGG 3 cut(s) 411, 866, 1131
Bse118I RCCGGY 1 cut(s) 599
Bse1I ACTGG 2 cut(s) 448, 828
Bse21I CCTNAGG 1 cut(s) 1126
Bse3DI GCAATG 3 cut(s) 123, 494, 1080
Bse8I GATNNNNATC 1 cut(s) 1019
BseBI CCWGG 4 cut(s) 203, 608, 864, 1116
BseCI ATCGAT 1 cut(s) 615
BseDI CCNNGG 1 cut(s) 132
BseGI GGATG 1 cut(s) 10
BseJI GATNNNNATC 1 cut(s) 1019
BseLI CCNNNNNNNGG 3 cut(s) 411, 866, 1131
BseMI GCAATG 3 cut(s) 123, 494, 1080
BseMII CTCAG 1 cut(s) 1140
BseNI ACTGG 2 cut(s) 448, 828
BseRI GAGGAG 1 cut(s) 609
BseXI GCAGC 3 cut(s) 252, 344, 713
BseYI CCCAGC 1 cut(s) 291
BsgI GTGCAG 1 cut(s) 654
Bsh1236I CGCG 1 cut(s) 630
BshFI GGCC 1 cut(s) 141
BshVI ATCGAT 1 cut(s) 615
BsiHKAI GWGCWC 1 cut(s) 652
BsiSI CCGG 2 cut(s) 521, 600
BsiWI CGTACG 1 cut(s) 1086
BslFI GGGAC 2 cut(s) 680, 916
BslI CCNNNNNNNGG 3 cut(s) 411, 866, 1131
BsmAI GTCTC 3 cut(s) 410, 648, 771
BsmFI GGGAC 2 cut(s) 680, 916
BsnI GGCC 1 cut(s) 141
Bsp1286I GDGCHC 1 cut(s) 652
Bsp1407I TGTACA 1 cut(s) 314
Bsp143I GATC 6 cut(s) 277, 307, 571, 612, 907, 1014
BspACI CCGC 7 cut(s) 630, 699, 801, 974, 1045, 1172, 1239
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 1 cut(s) 1139
BspDI ATCGAT 1 cut(s) 615
BspFNI CGCG 1 cut(s) 630
BspLI GGNNCC 2 cut(s) 598, 735
BspMAI CTGCAG 1 cut(s) 1233
BspMI ACCTGC 1 cut(s) 1244
BspPI GGATC 2 cut(s) 272, 902
BspQI GCTCTTC 1 cut(s) 327
BspTI CTTAAG 1 cut(s) 889
BsrBI CCGCTC 1 cut(s) 803
BsrDI GCAATG 3 cut(s) 123, 494, 1080
BsrFI RCCGGY 1 cut(s) 599
BsrGI TGTACA 1 cut(s) 314
BsrI ACTGG 2 cut(s) 448, 828
BssAI RCCGGY 1 cut(s) 599
BssECI CCNNGG 1 cut(s) 132
BssMI GATC 6 cut(s) 277, 307, 571, 612, 907, 1014
BssSI CACGAG 1 cut(s) 651
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 4 cut(s) 203, 608, 864, 1116
Bst4CI ACNGT 3 cut(s) 421, 542, 685
Bst6I CTCTTC 4 cut(s) 25, 298, 327, 1011
BstAFI CTTAAG 1 cut(s) 889
BstAUI TGTACA 1 cut(s) 314
BstBAI YACGTR 1 cut(s) 1086
BstC8I GCNNGC 4 cut(s) 63, 99, 139, 337
BstDEI CTNAG 2 cut(s) 167, 1126
BstEII GGTNACC 1 cut(s) 602
BstF5I GGATG 1 cut(s) 10
BstFNI CGCG 1 cut(s) 630
BstHHI GCGC 1 cut(s) 97
BstKTI GATC 6 cut(s) 280, 310, 574, 615, 910, 1017
BstMAI GTCTC 3 cut(s) 410, 648, 771
BstMBI GATC 6 cut(s) 277, 307, 571, 612, 907, 1014
BstMWI GCNNNNNNNGC 7 cut(s) 134, 464, 647, 800, 938, 1051, 1162
BstNI CCWGG 4 cut(s) 203, 608, 864, 1116
BstPI GGTNACC 1 cut(s) 602
BstSCI CCNGG 5 cut(s) 201, 520, 606, 862, 1114
BstSFI CTRYAG 1 cut(s) 1229
BstSNI TACGTA 1 cut(s) 1086
BstUI CGCG 1 cut(s) 630
BstV1I GCAGC 3 cut(s) 252, 344, 713
Bsu15I ATCGAT 1 cut(s) 615
Bsu36I CCTNAGG 1 cut(s) 1126
BsuI GTATCC 1 cut(s) 632
BsuRI GGCC 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 615
BtsCI GGATG 1 cut(s) 10
BtsI GCAGTG 2 cut(s) 846, 1092
BtsIMutI CAGTG 3 cut(s) 547, 846, 1092
BveI ACCTGC 1 cut(s) 1244
Cac8I GCNNGC 4 cut(s) 63, 99, 139, 337
CaiI CAGNNNCTG 1 cut(s) 61
CfoI GCGC 1 cut(s) 97
Cfr10I RCCGGY 1 cut(s) 599
Cfr13I GGNCC 1 cut(s) 733
ClaI ATCGAT 1 cut(s) 615
CseI GACGC 2 cut(s) 219, 800
CviAII CATG 4 cut(s) 143, 716, 730, 1012
DdeI CTNAG 2 cut(s) 167, 1126
DpnI GATC 6 cut(s) 279, 309, 573, 614, 909, 1016
DpnII GATC 6 cut(s) 277, 307, 571, 612, 907, 1014
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 4 cut(s) 25, 298, 327, 1011
EarI CTCTTC 4 cut(s) 25, 298, 327, 1011
Eco105I TACGTA 1 cut(s) 1086
Eco130I CCWWGG 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 733
Eco81I CCTNAGG 1 cut(s) 1126
Eco91I GGTNACC 1 cut(s) 602
EcoO65I GGTNACC 1 cut(s) 602
EcoRII CCWGG 4 cut(s) 201, 606, 862, 1114
EcoT14I CCWWGG 1 cut(s) 132
EcoT22I ATGCAT 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 132
FaeI CATG 4 cut(s) 146, 719, 733, 1015
FaqI GGGAC 2 cut(s) 680, 916
FatI CATG 4 cut(s) 142, 715, 729, 1011
FauI CCCGC 1 cut(s) 706
FbaI TGATCA 1 cut(s) 1014
Fnu4HI GCNGC 3 cut(s) 266, 333, 702
FokI GGATG 1 cut(s) 17
Fsp4HI GCNGC 3 cut(s) 266, 333, 702
FspBI CTAG 2 cut(s) 513, 591
GlaI GCGC 1 cut(s) 96
GluI GCNGC 3 cut(s) 266, 333, 702
GsaI CCCAGC 1 cut(s) 295
GsuI CTGGAG 3 cut(s) 629, 846, 1098
HaeIII GGCC 1 cut(s) 141
HapII CCGG 2 cut(s) 521, 600
HgaI GACGC 2 cut(s) 219, 800
HhaI GCGC 1 cut(s) 97
Hin1II CATG 4 cut(s) 146, 719, 733, 1015
Hin6I GCGC 1 cut(s) 95
HinP1I GCGC 1 cut(s) 95
HindIII AAGCTT 1 cut(s) 1202
HinfI GANTC 4 cut(s) 52, 285, 547, 779
HpaII CCGG 2 cut(s) 521, 600
HphI GGTGA 2 cut(s) 419, 614
Hpy166II GTNNAC 4 cut(s) 494, 538, 664, 1137
Hpy188I TCNGA 3 cut(s) 385, 552, 709
Hpy188III TCNNGA 5 cut(s) 27, 74, 105, 569, 785
Hpy8I GTNNAC 4 cut(s) 494, 538, 664, 1137
Hpy99I CGWCG 1 cut(s) 968
HpyAV CCTTC 1 cut(s) 635
HpyCH4III ACNGT 3 cut(s) 421, 542, 685
HpyCH4IV ACGT 4 cut(s) 534, 963, 1085, 1089
HpyCH4V TGCA 8 cut(s) 223, 332, 487, 635, 851, 1073, 1097, 1231
HpyF10VI GCNNNNNNNGC 7 cut(s) 134, 464, 647, 800, 938, 1051, 1162
HpyF3I CTNAG 2 cut(s) 167, 1126
HpySE526I ACGT 4 cut(s) 534, 963, 1085, 1089
Hsp92II CATG 4 cut(s) 146, 719, 733, 1015
HspAI GCGC 1 cut(s) 95
Ksp22I TGATCA 1 cut(s) 1014
Kzo9I GATC 6 cut(s) 277, 307, 571, 612, 907, 1014
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 4 cut(s) 262, 596, 638, 1051
Lsp1109I GCAGC 3 cut(s) 252, 344, 713
LweI GCATC 3 cut(s) 210, 454, 713
MaeI CTAG 2 cut(s) 513, 591
MaeII ACGT 4 cut(s) 534, 963, 1085, 1089
MaeIII GTNAC 3 cut(s) 400, 602, 1090
MalI GATC 6 cut(s) 279, 309, 573, 614, 909, 1016
MbiI CCGCTC 1 cut(s) 803
MboI GATC 6 cut(s) 277, 307, 571, 612, 907, 1014
MboII GAAGA 6 cut(s) 42, 314, 315, 407, 577, 998
MhlI GDGCHC 1 cut(s) 652
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 5 cut(s) 354, 476, 742, 1033, 1252
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 2 cut(s) 279, 773
MmeI TCCRAC 2 cut(s) 67, 1029
Mox20I TGGCCA 1 cut(s) 141
Mph1103I ATGCAT 1 cut(s) 225
MscI TGGCCA 1 cut(s) 141
MseI TTAA 8 cut(s) 156, 297, 360, 438, 504, 689, 890, 1255
Msp20I TGGCCA 1 cut(s) 141
MspCI CTTAAG 1 cut(s) 889
MspI CCGG 2 cut(s) 521, 600
MspR9I CCNGG 5 cut(s) 203, 522, 608, 864, 1116
MvaI CCWGG 4 cut(s) 203, 608, 864, 1116
MvnI CGCG 1 cut(s) 630
MwoI GCNNNNNNNGC 7 cut(s) 134, 464, 647, 800, 938, 1051, 1162
NciI CCSGG 1 cut(s) 522
NdeII GATC 6 cut(s) 277, 307, 571, 612, 907, 1014
NlaIII CATG 4 cut(s) 146, 719, 733, 1015
NlaIV GGNNCC 2 cut(s) 598, 735
NmuCI GTSAC 3 cut(s) 400, 602, 1090
NsiI ATGCAT 1 cut(s) 225
PciSI GCTCTTC 1 cut(s) 327
PfeI GAWTC 2 cut(s) 52, 547
Pfl23II CGTACG 1 cut(s) 1086
PflFI GACNNNGTC 1 cut(s) 659
PkrI GCNGC 3 cut(s) 267, 334, 703
PleI GAGTC 2 cut(s) 279, 773
PpsI GAGTC 2 cut(s) 279, 773
Ppu21I YACGTR 1 cut(s) 1086
PsiI TTATAA 1 cut(s) 948
Psp6I CCWGG 4 cut(s) 201, 606, 862, 1114
PspEI GGTNACC 1 cut(s) 602
PspFI CCCAGC 1 cut(s) 291
PspGI CCWGG 4 cut(s) 201, 606, 862, 1114
PspLI CGTACG 1 cut(s) 1086
PspN4I GGNNCC 2 cut(s) 598, 735
PspPI GGNCC 1 cut(s) 733
PsrI GAACNNNNNNTAC 2 cut(s) 746, 778
PstI CTGCAG 1 cut(s) 1233
PstNI CAGNNNCTG 1 cut(s) 61
PsyI GACNNNGTC 1 cut(s) 659
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 8 cut(s) 156, 297, 360, 438, 504, 689, 890, 1255
SatI GCNGC 3 cut(s) 266, 333, 702
Sau3AI GATC 6 cut(s) 277, 307, 571, 612, 907, 1014
Sau96I GGNCC 1 cut(s) 733
ScaI AGTACT 3 cut(s) 517, 755, 822
SchI GAGTC 2 cut(s) 279, 773
ScrFI CCNGG 5 cut(s) 203, 522, 608, 864, 1116
SduI GDGCHC 1 cut(s) 652
SfaNI GCATC 3 cut(s) 210, 454, 713
SfcI CTRYAG 1 cut(s) 1229
SinI GGWCC 1 cut(s) 733
SmlI CTYRAG 1 cut(s) 889
SmoI CTYRAG 1 cut(s) 889
SnaBI TACGTA 1 cut(s) 1086
SpeI ACTAGT 1 cut(s) 512
Sse9I AATT 5 cut(s) 354, 476, 742, 1033, 1252
SsiI CCGC 7 cut(s) 630, 699, 801, 974, 1045, 1172, 1239
SspMI CTAG 2 cut(s) 513, 591
StyD4I CCNGG 5 cut(s) 201, 520, 606, 862, 1114
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 421, 542, 685
TaiI ACGT 4 cut(s) 537, 966, 1088, 1092
TaqI TCGA 4 cut(s) 28, 393, 615, 1225
TaqII GACCGA 1 cut(s) 419
TasI AATT 5 cut(s) 354, 476, 742, 1033, 1252
TatI WGTACW 6 cut(s) 314, 515, 753, 820, 1077, 1147
TfiI GAWTC 2 cut(s) 52, 547
Tru1I TTAA 8 cut(s) 156, 297, 360, 438, 504, 689, 890, 1255
Tru9I TTAA 8 cut(s) 156, 297, 360, 438, 504, 689, 890, 1255
TscAI CASTG 3 cut(s) 547, 853, 1099
TseFI GTSAC 3 cut(s) 400, 602, 1090
TseI GCWGC 3 cut(s) 265, 332, 701
Tsp45I GTSAC 3 cut(s) 400, 602, 1090
TspDTI ATGAA 2 cut(s) 21, 314
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 3 cut(s) 547, 853, 1099
Tth111I GACNNNGTC 1 cut(s) 659
Vha464I CTTAAG 1 cut(s) 889
VpaK11BI GGWCC 1 cut(s) 733
XapI RAATTY 1 cut(s) 742
XcmI CCANNNNNNNNNTGG 2 cut(s) 244, 870
XspI CTAG 2 cut(s) 513, 591
ZrmI AGTACT 3 cut(s) 517, 755, 822
Zsp2I ATGCAT 1 cut(s) 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.