MD04G1235200.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003301
Physical Location & Seq
Forward (+)
31428517 .. 31429323
807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1235200.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGATCAAACACTGTACGAGGCTTCTCATGCAGGAAACATGCTACTGCTCTCGCGCCGAAACACCAATGGCGGTGGCGGCCAGCGTGACGTTTGCTTCCTTAACAAGACCTGTTTCGCCGAATTAAGAAACCAGGGCGGCTTCACTCCGATGCACATAGCGTCTGCGAACGGATACTTGGACATTGTGAAGGAGAATCTTTGCCGCTTCGGAGCGGCAGTCTGCCATCTCAGAGGAGGAAAACGTGACTTGACTCCTCTCCATTGCGCTGCCATCTGCGGCCGCGTTGAAATACTGAAAGAAATCATCTCCGCTTGTCCTGAGAGCCTTGAAGACGTAACCTTAAGCGGAGAGACCGCCTTTCACCTTGCTGCTAAAAACAACCGAGTCGAAGCGATTCATGTTTTGGTGGAACTGACGGTCGACTCACCAATGATCACGGTAGACATGAAGAGGAAGATCCTTAACATGAAGAACGTAACGGGGAATTCAGTTCTTCACGTTGCCATCACTGAACGGCGGCACGAGGTGGTGAGATGCTTGGTTGGGAGTGGAGTTACGGAATTAAATGATGTGAACGAGTGTGGTCTCACGGCTCTTGATCTGGTGCTGCGTCTCCCCAAGATTAGTAAATCATCGTACGCCGAGGATAAGAAAGCGGAGGATGTTCTTCGCAGCGGCGGAGCTTTGAGAGGGCGAGACTTGGCGGCAGTTAACTGGCTTTACATGCCATCAAAGGCAGAGGCCCGCCGTTCCGGGTTGCGAAAAAGAGTGACGCGGCTGGGTCAACTCTCGATCGTTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

269

Amino Acids

29.38

Weight (kDa)

8.97

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 52 - 141 9.2e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 113 - 185 5.3e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 215
AccI GTMKAC 2 cut(s) 423, 444
AccII CGCG 3 cut(s) 55, 285, 778
AclWI GGATC 2 cut(s) 12, 454
AcoI YGGCCR 2 cut(s) 79, 280
AcsI RAATTY 1 cut(s) 487
AdeI CACNNNGTG 1 cut(s) 529
AfaI GTAC 2 cut(s) 17, 641
AflII CTTAAG 1 cut(s) 343
AgsI TTSAA 2 cut(s) 290, 332
AjnI CCWGG 1 cut(s) 132
AjuI GAANNNNNNNTTGG 2 cut(s) 161, 193
AluBI AGCT 1 cut(s) 686
AluI AGCT 1 cut(s) 686
Alw26I GTCTC 4 cut(s) 347, 593, 620, 693
AlwI GGATC 2 cut(s) 12, 454
AoxI GGCC 3 cut(s) 79, 280, 744
ApeKI GCWGC 4 cut(s) 269, 371, 610, 675
ApoI RAATTY 1 cut(s) 487
Asp700I GAANNNNTTC 1 cut(s) 396
AspLEI GCGC 2 cut(s) 57, 269
AspS9I GGNCC 1 cut(s) 745
AsuC2I CCSGG 1 cut(s) 757
AsuHPI GGTGA 3 cut(s) 356, 420, 544
BauI CACGAG 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 339
BbvI GCAGC 4 cut(s) 256, 358, 597, 687
BccI CCATC 4 cut(s) 234, 281, 515, 739
BceAI ACGGC 3 cut(s) 533, 609, 735
BciT130I CCWGG 1 cut(s) 134
BciVI GTATCC 1 cut(s) 167
BclI TGATCA 1 cut(s) 435
BcnI CCSGG 1 cut(s) 757
BcoDI GTCTC 4 cut(s) 347, 593, 620, 693
BfrI CTTAAG 1 cut(s) 343
BfuI GTATCC 1 cut(s) 167
Bme1390I CCNGG 2 cut(s) 134, 757
BmgT120I GGNCC 1 cut(s) 745
BmrFI CCNGG 2 cut(s) 134, 757
BmsI GCATC 2 cut(s) 141, 527
BpiI GAAGAC 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 757
BsaI GGTCTC 2 cut(s) 347, 593
BsaJI CCNNGG 2 cut(s) 133, 645
BsaXI ACNNNNNCTCC 2 cut(s) 204, 234
Bse1I ACTGG 1 cut(s) 722
Bse3DI GCAATG 1 cut(s) 262
BseBI CCWGG 1 cut(s) 134
BseDI CCNNGG 2 cut(s) 133, 645
BseGI GGATG 1 cut(s) 670
BseMI GCAATG 1 cut(s) 262
BseMII CTCAG 2 cut(s) 244, 312
BseNI ACTGG 1 cut(s) 722
BseRI GAGGAG 2 cut(s) 246, 249
BseX3I CGGCCG 1 cut(s) 280
BseXI GCAGC 4 cut(s) 256, 358, 597, 687
BseYI CCCAGC 1 cut(s) 781
Bsh1236I CGCG 3 cut(s) 55, 285, 778
Bsh1285I CGRYCG 3 cut(s) 283, 423, 798
BshFI GGCC 3 cut(s) 81, 282, 746
BsiEI CGRYCG 3 cut(s) 283, 423, 798
BsiSI CCGG 1 cut(s) 756
BsiWI CGTACG 1 cut(s) 639
BsmAI GTCTC 4 cut(s) 347, 593, 620, 693
BsmBI CGTCTC 1 cut(s) 620
BsnI GGCC 3 cut(s) 81, 282, 746
Bso31I GGTCTC 2 cut(s) 347, 593
Bsp143I GATC 5 cut(s) 4, 435, 459, 601, 795
BspANI GGCC 3 cut(s) 81, 282, 746
BspCNI CTCAG 2 cut(s) 243, 313
BspFNI CGCG 3 cut(s) 55, 285, 778
BspPI GGATC 2 cut(s) 12, 454
BspTI CTTAAG 1 cut(s) 343
BspTNI GGTCTC 2 cut(s) 347, 593
BsrBI CCGCTC 1 cut(s) 215
BsrDI GCAATG 1 cut(s) 262
BsrI ACTGG 1 cut(s) 722
BssECI CCNNGG 2 cut(s) 133, 645
BssMI GATC 5 cut(s) 4, 435, 459, 601, 795
BssSI CACGAG 1 cut(s) 524
Bst2BI CACGAG 1 cut(s) 524
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 3 cut(s) 15, 421, 442
Bst6I CTCTTC 1 cut(s) 446
BstAFI CTTAAG 1 cut(s) 343
BstC8I GCNNGC 2 cut(s) 83, 748
BstDEI CTNAG 2 cut(s) 230, 321
BstF5I GGATG 1 cut(s) 670
BstFNI CGCG 3 cut(s) 55, 285, 778
BstHHI GCGC 2 cut(s) 57, 269
BstKTI GATC 5 cut(s) 7, 438, 462, 604, 798
BstMAI GTCTC 4 cut(s) 347, 593, 620, 693
BstMBI GATC 5 cut(s) 4, 435, 459, 601, 795
BstMCI CGRYCG 3 cut(s) 283, 423, 798
BstMWI GCNNNNNNNGC 3 cut(s) 29, 78, 727
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 2 cut(s) 43, 730
BstSCI CCNGG 2 cut(s) 132, 755
BstUI CGCG 3 cut(s) 55, 285, 778
BstV1I GCAGC 4 cut(s) 256, 358, 597, 687
BstV2I GAAGAC 1 cut(s) 339
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BstZI CGGCCG 1 cut(s) 280
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 3 cut(s) 81, 282, 746
BtsCI GGATG 1 cut(s) 670
BtsIMutI CAGTG 2 cut(s) 11, 510
Cac8I GCNNGC 2 cut(s) 83, 748
CciNI GCGGCCGC 1 cut(s) 280
CfoI GCGC 2 cut(s) 57, 269
Cfr13I GGNCC 1 cut(s) 745
CseI GACGC 3 cut(s) 150, 602, 784
Csp6I GTAC 2 cut(s) 16, 640
CspCI CAANNNNNGTGG 2 cut(s) 55, 90
CviAII CATG 6 cut(s) 29, 40, 401, 448, 469, 727
CviQI GTAC 2 cut(s) 16, 640
DdeI CTNAG 2 cut(s) 230, 321
DpnI GATC 5 cut(s) 6, 437, 461, 603, 797
DpnII GATC 5 cut(s) 4, 435, 459, 601, 795
DraIII CACNNNGTG 1 cut(s) 529
EaeI YGGCCR 2 cut(s) 79, 280
EagI CGGCCG 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 446
EarI CTCTTC 1 cut(s) 446
EciI GGCGGA 1 cut(s) 696
EclXI CGGCCG 1 cut(s) 280
Eco31I GGTCTC 2 cut(s) 347, 593
Eco52I CGGCCG 1 cut(s) 280
EcoRI GAATTC 1 cut(s) 487
EcoRII CCWGG 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 620
FaeI CATG 6 cut(s) 32, 43, 404, 451, 472, 730
FaiI YATR 7 cut(s) 30, 41, 158, 402, 449, 470, 728
FatI CATG 6 cut(s) 28, 39, 400, 447, 468, 726
FauI CCCGC 1 cut(s) 755
FbaI TGATCA 1 cut(s) 435
FblI GTMKAC 2 cut(s) 423, 444
FokI GGATG 1 cut(s) 677
GlaI GCGC 2 cut(s) 56, 268
GsaI CCCAGC 1 cut(s) 785
HaeIII GGCC 3 cut(s) 81, 282, 746
HapII CCGG 1 cut(s) 756
HgaI GACGC 3 cut(s) 150, 602, 784
HhaI GCGC 2 cut(s) 57, 269
Hin1II CATG 6 cut(s) 32, 43, 404, 451, 472, 730
Hin6I GCGC 2 cut(s) 55, 267
HinP1I GCGC 2 cut(s) 55, 267
HincII GTYRAC 3 cut(s) 424, 715, 788
HindII GTYRAC 3 cut(s) 424, 715, 788
HinfI GANTC 5 cut(s) 196, 253, 387, 397, 425
HpaI GTTAAC 1 cut(s) 715
HpaII CCGG 1 cut(s) 756
HphI GGTGA 3 cut(s) 356, 420, 544
Hpy166II GTNNAC 5 cut(s) 424, 445, 577, 715, 788
Hpy188I TCNGA 3 cut(s) 150, 212, 233
Hpy188III TCNNGA 3 cut(s) 320, 599, 793
Hpy8I GTNNAC 5 cut(s) 424, 445, 577, 715, 788
HpyAV CCTTC 1 cut(s) 184
HpyCH4III ACNGT 3 cut(s) 15, 421, 442
HpyCH4IV ACGT 5 cut(s) 90, 244, 336, 477, 501
HpyCH4V TGCA 2 cut(s) 32, 154
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 78, 727
HpyF3I CTNAG 2 cut(s) 230, 321
HpySE526I ACGT 5 cut(s) 90, 244, 336, 477, 501
Hsp92II CATG 6 cut(s) 32, 43, 404, 451, 472, 730
HspAI GCGC 2 cut(s) 55, 267
Ksp22I TGATCA 1 cut(s) 435
KspAI GTTAAC 1 cut(s) 715
Kzo9I GATC 5 cut(s) 4, 435, 459, 601, 795
LmnI GCTCC 2 cut(s) 212, 683
Lsp1109I GCAGC 4 cut(s) 256, 358, 597, 687
LweI GCATC 2 cut(s) 141, 527
MaeII ACGT 5 cut(s) 90, 244, 336, 477, 501
MaeIII GTNAC 6 cut(s) 86, 245, 337, 478, 556, 772
MalI GATC 5 cut(s) 6, 437, 461, 603, 797
MbiI CCGCTC 1 cut(s) 215
MboI GATC 5 cut(s) 4, 435, 459, 601, 795
MboII GAAGA 6 cut(s) 344, 463, 469, 484, 488, 662
MflI RGATCY 1 cut(s) 459
MluCI AATT 3 cut(s) 122, 487, 563
MlyI GAGTC 3 cut(s) 247, 396, 419
MroXI GAANNNNTTC 1 cut(s) 396
MseI TTAA 6 cut(s) 102, 125, 344, 465, 566, 714
MslI CAYNNNNRTG 1 cut(s) 149
MspA1I CMGCKG 1 cut(s) 678
MspCI CTTAAG 1 cut(s) 343
MspI CCGG 1 cut(s) 756
MspR9I CCNGG 2 cut(s) 134, 757
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 3 cut(s) 55, 285, 778
MwoI GCNNNNNNNGC 3 cut(s) 29, 78, 727
NciI CCSGG 1 cut(s) 757
NdeII GATC 5 cut(s) 4, 435, 459, 601, 795
NlaIII CATG 6 cut(s) 32, 43, 404, 451, 472, 730
NmeAIII GCCGAG 1 cut(s) 670
NmuCI GTSAC 3 cut(s) 86, 245, 772
NotI GCGGCCGC 1 cut(s) 280
NspI RCATGY 2 cut(s) 43, 730
PdmI GAANNNNTTC 1 cut(s) 396
PfeI GAWTC 2 cut(s) 196, 397
Pfl23II CGTACG 1 cut(s) 639
Ple19I CGATCG 1 cut(s) 798
PleI GAGTC 3 cut(s) 247, 395, 419
PpsI GAGTC 3 cut(s) 247, 395, 419
Psp6I CCWGG 1 cut(s) 132
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 1 cut(s) 132
PspLI CGTACG 1 cut(s) 639
PspPI GGNCC 1 cut(s) 745
PsuI RGATCY 1 cut(s) 459
PvuI CGATCG 1 cut(s) 798
RsaI GTAC 2 cut(s) 17, 641
RsaNI GTAC 2 cut(s) 16, 640
RseI CAYNNNNRTG 1 cut(s) 149
SalI GTCGAC 1 cut(s) 422
SaqAI TTAA 6 cut(s) 102, 125, 344, 465, 566, 714
Sau3AI GATC 5 cut(s) 4, 435, 459, 601, 795
Sau96I GGNCC 1 cut(s) 745
SchI GAGTC 3 cut(s) 247, 396, 419
ScrFI CCNGG 2 cut(s) 134, 757
SfaNI GCATC 2 cut(s) 141, 527
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 1 cut(s) 343
SmoI CTYRAG 1 cut(s) 343
Sse9I AATT 3 cut(s) 122, 487, 563
StyD4I CCNGG 2 cut(s) 132, 755
TaaI ACNGT 3 cut(s) 15, 421, 442
TaiI ACGT 5 cut(s) 93, 247, 339, 480, 504
TaqI TCGA 3 cut(s) 390, 423, 794
TasI AATT 3 cut(s) 122, 487, 563
TfiI GAWTC 2 cut(s) 196, 397
Tru1I TTAA 6 cut(s) 102, 125, 344, 465, 566, 714
Tru9I TTAA 6 cut(s) 102, 125, 344, 465, 566, 714
TscAI CASTG 2 cut(s) 18, 517
TseFI GTSAC 3 cut(s) 86, 245, 772
TseI GCWGC 4 cut(s) 269, 371, 610, 675
Tsp45I GTSAC 3 cut(s) 86, 245, 772
TspDTI ATGAA 3 cut(s) 389, 464, 485
TspGWI ACGGA 2 cut(s) 186, 575
TspRI CASTG 2 cut(s) 18, 517
Vha464I CTTAAG 1 cut(s) 343
XapI RAATTY 1 cut(s) 487
XceI RCATGY 2 cut(s) 43, 730
XmiI GTMKAC 2 cut(s) 423, 444
XmnI GAANNNNTTC 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.