Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003556

Physical Location & Seq

Genomic Coordinates Forward (+)

22269859 .. 22271777

Gene Structure 1919 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G54820.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1419 bp

ATGGATTCAATTCAACAAGACAGATTATCTAGTCTTCCCGACATTCTTCTAATTATGATCATCTCTTTCTTGCCGTTAAAGGAATGTGTAAGGACCAGTGTTCTCTCCAAGAGGTGGAGGTATCTCTGCTTGGAGACCACAAACCTTTCTTTTAAGGAATCTGATTACGTGAATCCTGATATCACCGATGCTGAATATTCGAGAATTGTAGCTTATCGTTCATTCTTTTGTTCTGTGGATAAATGGGTCTCTATAACTCAACATCAAGTCGTCGAAAGCTTCGAGATTTGTTTCTCCCATCTGGTGGGTTTCGAGGATAAGATCGACGCTCTGATCGAATACGCTGTCTCCACACGAGTGAAGAATTTAGTTGTTGATTTGTCAAACCCCTCTTGGAGAAGCAATGGTGATATATCATATCGTCATTTTATGTATACATTGCCTAAAAGCGTTTACAGTCTAACAACCTTGGAGTCACTGAAGATTTACGGGTGCAAGTTTGATCCTTCGAAATTCGTAAACCCTGTATTGCTAAGAAGCCTCTCTATTGGTTGGGTCAGACTCGAAAACCTCCACTCTTTACTATCCAAATCTCCTTCCCTCCAGAGTCTAAGTATCAAAAATTGTTGGGGCGTTGATATCACGTCGATGGCCGGGCAGTTCAGAGAGCTAGTCATTGAGCATTCAGACTTCAGTTACATGCAATGTGCCTTTGAGCTGCCTCGTATACACAGCTTCAAGTACTCTGGGGAGTTGTTCGAGTTCTACTTCGACGTAGTGAATGTGATCATACCAAATGTGTACTTGGATTTTGGAGAAGAGCGAGTATATGATCTACAAAGCCAATCATCAAGAATATCGGGTGAGGTTATTTCCCGTATCATTAACGACCTTAGAGCAGCTGAGACTTTAACTGTCTGCCCTTATATCCTCCAGGTTATCCCAGAATGTGAAAAGCCATCTGATTTGCTTCAACCTATGGAAACACGACACTTGGTGTTAAGAACTAAGATGCATACCAAGGAGTTCAATGGAATAATTCTTCTTCTCAACAACTGCCCTAATTTGGAGACGCTTGGTTTTGATATTCTAACTCCATGTCCTTTCTCGGCAACTTCGTCAGATGAAGGCATCGATCCTAAAACGTATTGGATGCAGAAGAGAACATGTAAGAGTCTAAGGAAGACACTGAAGGTCGTGGTTATTAGGAACTTTTGTGGTTCCTCAAACGAGCTAAATGTATTGAGGTATTTGATTCGATCAGCGAGCGGTGCTGGAGATGCGTTGGAAAGAGTAGAGCTTTACGTGCCGAATGGGATGGAAGAAAGTCAAGCGATGGTGGTATTTGCTAAAGCGGAAATGTTACAGAGAACTTCGAAGCATGTACAAGTTCTTTGTGCACAACTCTTGAAAAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0007276 GO:0008150 GO:0009555 GO:0009987 GO:0019953 GO:0022412 GO:0022414 GO:0030154 GO:0032501 GO:0032502 GO:0032504 GO:0044703 GO:0048229 GO:0048232 GO:0048235 GO:0048609 GO:0048856 GO:0048869 GO:0051704 GO:0055046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBD

Protein Analysis

472

Amino Acids

54.08

Weight (kDa)

6.34

Isoelectric Point (pI)

42.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	9 - 43	3.4e-07	F-box domain
LRR_At1g61320_AtMIF1	PF23622	106 - 409	5.2e-08	At1g61320/AtMIF1, LRR domain
LRR_At5g56370	PF24758	144 - 214	1.5e-06	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003556)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G56400 AT1G56400 AT2G17305 AT3G29830 AT3G29830 AT3G62230 AT5G54820
fragaria_vesca	FvH4_7g19741
malus_domestica	MD03G1208600.v1.1 MD11G1222600.v1.1 MD11G1222800.v1.1 MD11G1223200.v1.1
prunus_persica	Prupe.4G208100_v2.0.a1 Prupe.4G208200_v2.0.a1 Prupe.4G208300_v2.0.a1
pyrus_communis	pycom03g15720 pycom03g15760 pycom11g19660
rosa_chinensis	RchiOBHm_Chr1g0363631 RchiOBHm_Chr7g0214121
rosa_laevigata	RLG00000008357 RLG00000027555
rosa_multiflora	Rmu_sc0000548.1_g000010 Rmu_sc0000675.1_g000034
rosa_roxburghii	Rroxscaffold_4G00293030
rosa_rugosa	Rorug01G0308300
rosa_samantha	Rh1AG089100 Rh1AG317200 Rh1BG280600 Rh1CG296700 Rh1DG311800 Rh4AG168100 Rh4BG165000
rosa_wichuraiana	Rw1G006910 Rw1G028130

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	114, 304
AccBSI	CCGCTC	1 cut(s)	1269
AccI	GTMKAC	2 cut(s)	434, 727
AciI	CCGC	2 cut(s)	1269, 1355
AclWI	GGATC	2 cut(s)	497, 1130
AcoI	YGGCCR	1 cut(s)	651
AcsI	RAATTY	2 cut(s)	364, 512
AcuI	CTGAAG	3 cut(s)	500, 676, 1211
AdeI	CACNNNGTG	1 cut(s)	997
AfaI	GTAC	3 cut(s)	743, 803, 1386
AfiI	CCNNNNNNNGG	3 cut(s)	114, 304, 1066
AflIII	ACRYGT	1 cut(s)	1166
AgsI	TTSAA	6 cut(s)	9, 14, 739, 974, 1030, 1411
AjiI	CACGTC	1 cut(s)	645
AjnI	CCWGG	1 cut(s)	933
AleI	CACNNNNGTG	1 cut(s)	356
AluBI	AGCT	8 cut(s)	212, 279, 670, 718, 735, 902, 1234, 1300
AluI	AGCT	8 cut(s)	212, 279, 670, 718, 735, 902, 1234, 1300
Alw21I	GWGCWC	1 cut(s)	1402
Alw26I	GTCTC	5 cut(s)	128, 253, 352, 899, 1064
Alw44I	GTGCAC	1 cut(s)	1398
AlwI	GGATC	2 cut(s)	497, 1130
AoxI	GGCC	1 cut(s)	651
ApaLI	GTGCAC	1 cut(s)	1398
ApeKI	GCWGC	2 cut(s)	718, 899
ApoI	RAATTY	2 cut(s)	364, 512
ArsI	GACNNNNNNTTYG	2 cut(s)	330, 362
AspS9I	GGNCC	1 cut(s)	93
AsuC2I	CCSGG	1 cut(s)	655
AsuHPI	GGTGA	3 cut(s)	175, 419, 875
AsuII	TTCGAA	2 cut(s)	509, 1376
AvaII	GGWCC	1 cut(s)	93
BaeGI	GKGCMC	1 cut(s)	1402
BauI	CACGAG	1 cut(s)	354
BbsI	GAAGAC	2 cut(s)	26, 1190
Bbv12I	GWGCWC	1 cut(s)	1402
BbvI	GCAGC	2 cut(s)	705, 911
BccI	CCATC	5 cut(s)	306, 643, 967, 1312, 1330
BceAI	ACGGC	1 cut(s)	58
BciT130I	CCWGG	1 cut(s)	935
BclI	TGATCA	2 cut(s)	57, 786
BcnI	CCSGG	1 cut(s)	655
BcoDI	GTCTC	5 cut(s)	128, 253, 352, 899, 1064
BfaI	CTAG	2 cut(s)	30, 671
BisI	GCNGC	2 cut(s)	719, 900
BlsI	GCNGC	2 cut(s)	720, 901
BmcAI	AGTACT	1 cut(s)	743
Bme1390I	CCNGG	2 cut(s)	655, 935
Bme18I	GGWCC	1 cut(s)	93
BmgBI	CACGTC	1 cut(s)	645
BmgT120I	GGNCC	1 cut(s)	93
BmiI	GGNNCC	1 cut(s)	1222
BmrFI	CCNGG	2 cut(s)	655, 935
BmsI	GCATC	5 cut(s)	178, 1002, 1140, 1143, 1270
BpiI	GAAGAC	2 cut(s)	26, 1190
BpmI	CTGGAG	3 cut(s)	587, 917, 1296
Bpu14I	TTCGAA	2 cut(s)	509, 1376
BpuMI	CCSGG	1 cut(s)	655
Bsa29I	ATCGAT	1 cut(s)	1134
BsaAI	YACGTR	2 cut(s)	169, 1306
BsaI	GGTCTC	2 cut(s)	128, 253
BsaJI	CCNNGG	2 cut(s)	468, 1020
BsaXI	ACNNNNNCTCC	2 cut(s)	332, 362
Bsc4I	CCNNNNNNNGG	3 cut(s)	114, 304, 1066
Bse1I	ACTGG	1 cut(s)	96
Bse3DI	GCAATG	3 cut(s)	409, 437, 710
BseBI	CCWGG	1 cut(s)	935
BseCI	ATCGAT	1 cut(s)	1134
BseDI	CCNNGG	2 cut(s)	468, 1020
BseGI	GGATG	2 cut(s)	1158, 1323
BseLI	CCNNNNNNNGG	3 cut(s)	114, 304, 1066
BseMI	GCAATG	3 cut(s)	409, 437, 710
BseMII	CTCAG	1 cut(s)	894
BseNI	ACTGG	1 cut(s)	96
BseSI	GKGCMC	1 cut(s)	1402
BseXI	GCAGC	2 cut(s)	705, 911
BshFI	GGCC	1 cut(s)	653
BshVI	ATCGAT	1 cut(s)	1134
BsiHKAI	GWGCWC	1 cut(s)	1402
BsiSI	CCGG	1 cut(s)	654
BslI	CCNNNNNNNGG	3 cut(s)	114, 304, 1066
BsmAI	GTCTC	5 cut(s)	128, 253, 352, 899, 1064
BsmBI	CGTCTC	1 cut(s)	1064
BsmI	GAATGC	1 cut(s)	682
BsnI	GGCC	1 cut(s)	653
Bso31I	GGTCTC	2 cut(s)	128, 253
Bsp119I	TTCGAA	2 cut(s)	509, 1376
Bsp1286I	GDGCHC	1 cut(s)	1402
Bsp1407I	TGTACA	1 cut(s)	1384
Bsp143I	GATC	8 cut(s)	57, 321, 333, 502, 786, 832, 1135, 1259
BspACI	CCGC	2 cut(s)	1269, 1355
BspANI	GGCC	1 cut(s)	653
BspCNI	CTCAG	1 cut(s)	895
BspDI	ATCGAT	1 cut(s)	1134
BspLI	GGNNCC	1 cut(s)	1222
BspPI	GGATC	2 cut(s)	497, 1130
BspQI	GCTCTTC	1 cut(s)	813
BspT104I	TTCGAA	2 cut(s)	509, 1376
BspTNI	GGTCTC	2 cut(s)	128, 253
BsrBI	CCGCTC	1 cut(s)	1269
BsrDI	GCAATG	3 cut(s)	409, 437, 710
BsrGI	TGTACA	1 cut(s)	1384
BsrI	ACTGG	1 cut(s)	96
BssECI	CCNNGG	2 cut(s)	468, 1020
BssMI	GATC	8 cut(s)	57, 321, 333, 502, 786, 832, 1135, 1259
BssNAI	GTATAC	2 cut(s)	435, 728
BssSI	CACGAG	1 cut(s)	354
BssT1I	CCWWGG	2 cut(s)	468, 1020
Bst1107I	GTATAC	2 cut(s)	435, 728
Bst2BI	CACGAG	1 cut(s)	354
Bst2UI	CCWGG	1 cut(s)	935
Bst4CI	ACNGT	2 cut(s)	458, 916
Bst6I	CTCTTC	2 cut(s)	813, 1154
BstAUI	TGTACA	1 cut(s)	1384
BstBAI	YACGTR	2 cut(s)	169, 1306
BstBI	TTCGAA	2 cut(s)	509, 1376
BstC8I	GCNNGC	1 cut(s)	1267
BstDEI	CTNAG	6 cut(s)	533, 611, 893, 903, 1008, 1178
BstF5I	GGATG	2 cut(s)	1158, 1323
BstKTI	GATC	8 cut(s)	60, 324, 336, 505, 789, 835, 1138, 1262
BstMAI	GTCTC	5 cut(s)	128, 253, 352, 899, 1064
BstMBI	GATC	8 cut(s)	57, 321, 333, 502, 786, 832, 1135, 1259
BstMWI	GCNNNNNNNGC	3 cut(s)	1271, 1280, 1306
BstNI	CCWGG	1 cut(s)	935
BstNSI	RCATGY	3 cut(s)	703, 1170, 1385
BstSCI	CCNGG	2 cut(s)	653, 933
BstSLI	GKGCMC	1 cut(s)	1402
BstV1I	GCAGC	2 cut(s)	705, 911
BstV2I	GAAGAC	2 cut(s)	26, 1190
BstZ17I	GTATAC	2 cut(s)	435, 728
Bsu15I	ATCGAT	1 cut(s)	1134
BsuRI	GGCC	1 cut(s)	653
BsuTUI	ATCGAT	1 cut(s)	1134
BtgZI	GCGATG	1 cut(s)	1349
BtrI	CACGTC	1 cut(s)	645
BtsCI	GGATG	2 cut(s)	1158, 1323
BtsIMutI	CAGTG	3 cut(s)	103, 476, 1187
Cac8I	GCNNGC	1 cut(s)	1267
Cfr13I	GGNCC	1 cut(s)	93
ClaI	ATCGAT	1 cut(s)	1134
CseI	GACGC	2 cut(s)	335, 1081
Csp6I	GTAC	3 cut(s)	742, 802, 1385
CviAII	CATG	4 cut(s)	700, 1098, 1167, 1382
CviQI	GTAC	3 cut(s)	742, 802, 1385
DdeI	CTNAG	6 cut(s)	533, 611, 893, 903, 1008, 1178
DpnI	GATC	8 cut(s)	59, 323, 335, 504, 788, 834, 1137, 1261
DpnII	GATC	8 cut(s)	57, 321, 333, 502, 786, 832, 1135, 1259
DraIII	CACNNNGTG	1 cut(s)	997
EaeI	YGGCCR	1 cut(s)	651
Eam1104I	CTCTTC	2 cut(s)	813, 1154
EarI	CTCTTC	2 cut(s)	813, 1154
Eco130I	CCWWGG	2 cut(s)	468, 1020
Eco31I	GGTCTC	2 cut(s)	128, 253
Eco32I	GATATC	2 cut(s)	181, 640
Eco47I	GGWCC	1 cut(s)	93
Eco57I	CTGAAG	3 cut(s)	500, 676, 1211
EcoRII	CCWGG	1 cut(s)	933
EcoRV	GATATC	2 cut(s)	181, 640
EcoT14I	CCWWGG	2 cut(s)	468, 1020
EcoT22I	ATGCAT	1 cut(s)	1017
ErhI	CCWWGG	2 cut(s)	468, 1020
Esp3I	CGTCTC	1 cut(s)	1064
FaeI	CATG	4 cut(s)	703, 1101, 1170, 1385
FatI	CATG	4 cut(s)	699, 1097, 1166, 1381
FbaI	TGATCA	2 cut(s)	57, 786
FblI	GTMKAC	2 cut(s)	434, 727
Fnu4HI	GCNGC	2 cut(s)	719, 900
FokI	GGATG	2 cut(s)	1165, 1330
Fsp4HI	GCNGC	2 cut(s)	719, 900
FspBI	CTAG	2 cut(s)	30, 671
GluI	GCNGC	2 cut(s)	719, 900
GsuI	CTGGAG	3 cut(s)	587, 917, 1296
HaeIII	GGCC	1 cut(s)	653
HapII	CCGG	1 cut(s)	654
HgaI	GACGC	2 cut(s)	335, 1081
Hin1II	CATG	4 cut(s)	703, 1101, 1170, 1385
HindIII	AAGCTT	1 cut(s)	277
HinfI	GANTC	8 cut(s)	5, 158, 172, 473, 561, 607, 1174, 1255
HpaII	CCGG	1 cut(s)	654
HphI	GGTGA	3 cut(s)	175, 419, 875
Hpy166II	GTNNAC	6 cut(s)	435, 454, 520, 728, 802, 1400
Hpy188I	TCNGA	7 cut(s)	163, 333, 560, 665, 688, 964, 1123
Hpy188III	TCNNGA	7 cut(s)	38, 176, 201, 283, 604, 852, 1408
Hpy8I	GTNNAC	6 cut(s)	435, 454, 520, 728, 802, 1400
Hpy99I	CGWCG	4 cut(s)	275, 329, 649, 776
HpyAV	CCTTC	4 cut(s)	516, 606, 1121, 1186
HpyCH4III	ACNGT	2 cut(s)	458, 916
HpyCH4IV	ACGT	5 cut(s)	168, 644, 774, 1145, 1305
HpyCH4V	TGCA	5 cut(s)	495, 703, 1015, 1156, 1400
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1271, 1280, 1306
HpyF3I	CTNAG	6 cut(s)	533, 611, 893, 903, 1008, 1178
HpySE526I	ACGT	5 cut(s)	168, 644, 774, 1145, 1305
Hsp92II	CATG	4 cut(s)	703, 1101, 1170, 1385
Ksp22I	TGATCA	2 cut(s)	57, 786
Kzo9I	GATC	8 cut(s)	57, 321, 333, 502, 786, 832, 1135, 1259
LguI	GCTCTTC	1 cut(s)	813
Lsp1109I	GCAGC	2 cut(s)	705, 911
LweI	GCATC	5 cut(s)	178, 1002, 1140, 1143, 1270
MaeI	CTAG	2 cut(s)	30, 671
MaeII	ACGT	5 cut(s)	168, 644, 774, 1145, 1305
MaeIII	GTNAC	3 cut(s)	474, 695, 1362
MalI	GATC	8 cut(s)	59, 323, 335, 504, 788, 834, 1137, 1261
MbiI	CCGCTC	1 cut(s)	1269
MboI	GATC	8 cut(s)	57, 321, 333, 502, 786, 832, 1135, 1259
MhlI	GDGCHC	1 cut(s)	1402
MluCI	AATT	8 cut(s)	9, 51, 204, 364, 512, 622, 1038, 1063
MlyI	GAGTC	4 cut(s)	482, 555, 616, 1183
MmeI	TCCRAC	1 cut(s)	1266
Mph1103I	ATGCAT	1 cut(s)	1017
MseI	TTAA	5 cut(s)	77, 153, 885, 911, 1001
MslI	CAYNNNNRTG	2 cut(s)	356, 647
MspA1I	CMGCKG	1 cut(s)	902
MspI	CCGG	1 cut(s)	654
MspR9I	CCNGG	2 cut(s)	655, 935
Mva1269I	GAATGC	1 cut(s)	682
MvaI	CCWGG	1 cut(s)	935
MwoI	GCNNNNNNNGC	3 cut(s)	1271, 1280, 1306
NciI	CCSGG	1 cut(s)	655
NdeII	GATC	8 cut(s)	57, 321, 333, 502, 786, 832, 1135, 1259
NlaIII	CATG	4 cut(s)	703, 1101, 1170, 1385
NlaIV	GGNNCC	1 cut(s)	1222
NmeAIII	GCCGAG	1 cut(s)	1088
NmuCI	GTSAC	1 cut(s)	474
NsiI	ATGCAT	1 cut(s)	1017
NspI	RCATGY	3 cut(s)	703, 1170, 1385
NspV	TTCGAA	2 cut(s)	509, 1376
OliI	CACNNNNGTG	1 cut(s)	356
PciI	ACATGT	1 cut(s)	1166
PciSI	GCTCTTC	1 cut(s)	813
PcsI	WCGNNNNNNNCGW	3 cut(s)	279, 333, 1115
PctI	GAATGC	1 cut(s)	682
PfeI	GAWTC	4 cut(s)	5, 158, 172, 1255
PflMI	CCANNNNNTGG	2 cut(s)	114, 304
PkrI	GCNGC	2 cut(s)	720, 901
PleI	GAGTC	4 cut(s)	481, 555, 615, 1182
PpsI	GAGTC	4 cut(s)	481, 555, 615, 1182
Ppu21I	YACGTR	2 cut(s)	169, 1306
PscI	ACATGT	1 cut(s)	1166
Psp6I	CCWGG	1 cut(s)	933
PspGI	CCWGG	1 cut(s)	933
PspN4I	GGNNCC	1 cut(s)	1222
PspPI	GGNCC	1 cut(s)	93
PvuII	CAGCTG	1 cut(s)	902
RsaI	GTAC	3 cut(s)	743, 803, 1386
RsaNI	GTAC	3 cut(s)	742, 802, 1385
RseI	CAYNNNNRTG	2 cut(s)	356, 647
SapI	GCTCTTC	1 cut(s)	813
SaqAI	TTAA	5 cut(s)	77, 153, 885, 911, 1001
SatI	GCNGC	2 cut(s)	719, 900
Sau3AI	GATC	8 cut(s)	57, 321, 333, 502, 786, 832, 1135, 1259
Sau96I	GGNCC	1 cut(s)	93
ScaI	AGTACT	1 cut(s)	743
SchI	GAGTC	4 cut(s)	482, 555, 616, 1183
ScrFI	CCNGG	2 cut(s)	655, 935
SduI	GDGCHC	1 cut(s)	1402
SfaNI	GCATC	5 cut(s)	178, 1002, 1140, 1143, 1270
SfuI	TTCGAA	2 cut(s)	509, 1376
SinI	GGWCC	1 cut(s)	93
SmiMI	CAYNNNNRTG	2 cut(s)	356, 647
Sse9I	AATT	8 cut(s)	9, 51, 204, 364, 512, 622, 1038, 1063
SsiI	CCGC	2 cut(s)	1269, 1355
SspI	AATATT	1 cut(s)	197
SspMI	CTAG	2 cut(s)	30, 671
StyD4I	CCNGG	2 cut(s)	653, 933
StyI	CCWWGG	2 cut(s)	468, 1020
TaaI	ACNGT	2 cut(s)	458, 916
TaiI	ACGT	5 cut(s)	171, 647, 777, 1148, 1308
TasI	AATT	8 cut(s)	9, 51, 204, 364, 512, 622, 1038, 1063
TatI	WGTACW	3 cut(s)	741, 801, 1384
TfiI	GAWTC	4 cut(s)	5, 158, 172, 1255
Tru1I	TTAA	5 cut(s)	77, 153, 885, 911, 1001
Tru9I	TTAA	5 cut(s)	77, 153, 885, 911, 1001
TscAI	CASTG	3 cut(s)	103, 483, 1194
TseFI	GTSAC	1 cut(s)	474
TseI	GCWGC	2 cut(s)	718, 899
Tsp45I	GTSAC	1 cut(s)	474
TspDTI	ATGAA	2 cut(s)	210, 1140
TspRI	CASTG	3 cut(s)	103, 483, 1194
Van91I	CCANNNNNTGG	2 cut(s)	114, 304
VneI	GTGCAC	1 cut(s)	1398
VpaK11BI	GGWCC	1 cut(s)	93
XapI	RAATTY	2 cut(s)	364, 512
XceI	RCATGY	3 cut(s)	703, 1170, 1385
XmiI	GTMKAC	2 cut(s)	434, 727
XspI	CTAG	2 cut(s)	30, 671
ZrmI	AGTACT	1 cut(s)	743
Zsp2I	ATGCAT	1 cut(s)	1017

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.