Rroxscaffold_4G00293030

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0003556
Physical Location & Seq
Forward (+)
13273382 .. 13274220
839 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00293030.1

Sequence Viewer

Length: 633 bp
ATGGCTGTGTTCGATATCGAAGCTGGGGAGTTGGAAGAGGCCTACCTTGACTTTGGACTCGAGTATGAAGGCAACGAAGGAATGGGGGACCTCCTCTATCAACTCCTTCACCAACTTCCTGTTAGAACACTCACAGTATGCACGTATATGCTTCAGGTTCTGCCATTGGAACTGATATCCAATGGTGTAATTCCTGTCTTCGGCGTGGCGAAGTTAACTCTGAGGACCGCAATGCTCAGTTGTGAGCAGAAAGGGGTCGAGTATCTCCTCAGAAGTTGCCCTGTCTTGCAAGCTTTGACCATCCAAATAGTCCCTCAGAGACTAGTTGATCTTGATGAAGGTCAACCGGATGATGTACGTTATGTATGGACTCCAAATGCACCGGCGTACCATTGTATAAACCAAACATTGAGAGTTGTACAGGTGAAAGGTTACACAGGTACGAGACACGAAATTGGCTTTCTGAAGTATCTCTTTTGCCGTGGCCGTGTGCTCGAAAAACTTCATATTAGTATCGGCGAGGAGCTTTTGAACCAGAGGAGTATGGCTGAAAATCGGCAACTTGTAATTCAAACATTTCAAGGTTTGAGAAGAGCTTCAAATAATCTCCGAATAAATACATATGGTAGATGA

Protein Analysis

210

Amino Acids

23.9

Weight (kDa)

5.84

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBD PF08387 131 - 170 2.7e-08 FBD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 228
AcoI YGGCCR 1 cut(s) 484
AcuI CTGAAG 2 cut(s) 137, 485
AfaI GTAC 4 cut(s) 357, 389, 420, 442
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 4 cut(s) 532, 572, 581, 600
AhlI ACTAGT 1 cut(s) 322
AluBI AGCT 4 cut(s) 23, 293, 526, 596
AluI AGCT 4 cut(s) 23, 293, 526, 596
Alw21I GWGCWC 1 cut(s) 495
Alw26I GTCTC 2 cut(s) 313, 439
AlwNI CAGNNNCTG 1 cut(s) 160
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 2 cut(s) 39, 484
Asp700I GAANNNNTTC 2 cut(s) 501, 595
AspS9I GGNCC 2 cut(s) 88, 225
AsuHPI GGTGA 2 cut(s) 101, 436
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 2 cut(s) 88, 225
BaeI ACNNNNGTAYC 2 cut(s) 371, 404
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 495
BccI CCATC 1 cut(s) 308
BceAI ACGGC 2 cut(s) 465, 471
BcoDI GTCTC 2 cut(s) 313, 439
BcuI ACTAGT 1 cut(s) 322
BfaI CTAG 1 cut(s) 323
Bme18I GGWCC 2 cut(s) 88, 225
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 88, 225
BmiI GGNNCC 1 cut(s) 89
BpiI GAAGAC 1 cut(s) 190
BsaAI YACGTR 1 cut(s) 144
BsaJI CCNNGG 1 cut(s) 481
BsaWI WCCGGW 1 cut(s) 346
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 481
BseGI GGATG 2 cut(s) 300, 355
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMI GCAATG 1 cut(s) 237
BseMII CTCAG 4 cut(s) 212, 250, 283, 329
BseRI GAGGAG 4 cut(s) 83, 257, 536, 553
BseYI CCCAGC 1 cut(s) 23
BshFI GGCC 2 cut(s) 41, 486
BsiHKAI GWGCWC 1 cut(s) 495
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 2 cut(s) 347, 383
BslFI GGGAC 2 cut(s) 101, 296
BslI CCNNNNNNNGG 1 cut(s) 200
BsmAI GTCTC 2 cut(s) 313, 439
BsmFI GGGAC 2 cut(s) 101, 296
BsnI GGCC 2 cut(s) 41, 486
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 495
Bsp1407I TGTACA 1 cut(s) 418
Bsp143I GATC 1 cut(s) 328
BspACI CCGC 1 cut(s) 228
BspANI GGCC 2 cut(s) 41, 486
BspCNI CTCAG 4 cut(s) 213, 249, 282, 328
BspLI GGNNCC 1 cut(s) 89
BspQI GCTCTTC 1 cut(s) 586
BsrDI GCAATG 1 cut(s) 237
BsrFI RCCGGY 1 cut(s) 382
BsrGI TGTACA 1 cut(s) 418
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 1 cut(s) 481
BssMI GATC 1 cut(s) 328
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 2 cut(s) 30, 586
BstAUI TGTACA 1 cut(s) 418
BstBAI YACGTR 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 291
BstDEI CTNAG 4 cut(s) 221, 236, 269, 315
BstDSI CCRYGG 1 cut(s) 481
BstF5I GGATG 2 cut(s) 300, 355
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 2 cut(s) 313, 439
BstMBI GATC 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 190
BsuRI GGCC 2 cut(s) 41, 486
BtgI CCRYGG 1 cut(s) 481
BtsCI GGATG 2 cut(s) 300, 355
Cac8I GCNNGC 1 cut(s) 291
CaiI CAGNNNCTG 1 cut(s) 160
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 2 cut(s) 88, 225
Csp6I GTAC 4 cut(s) 356, 388, 419, 441
CviJI RGCY 9 cut(s) 5, 23, 41, 293, 459, 486, 526, 548, 596
CviKI_1 RGCY 9 cut(s) 5, 23, 41, 293, 459, 486, 526, 548, 596
CviQI GTAC 4 cut(s) 356, 388, 419, 441
DdeI CTNAG 4 cut(s) 221, 236, 269, 315
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 30, 586
EarI CTCTTC 2 cut(s) 30, 586
Eco147I AGGCCT 1 cut(s) 41
Eco32I GATATC 2 cut(s) 16, 177
Eco47I GGWCC 2 cut(s) 88, 225
Eco57I CTGAAG 2 cut(s) 137, 485
Eco88I CYCGRG 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 88
EcoRV GATATC 2 cut(s) 16, 177
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 2 cut(s) 101, 296
FauNDI CATATG 1 cut(s) 622
FokI GGATG 2 cut(s) 287, 362
FspBI CTAG 1 cut(s) 323
GsaI CCCAGC 1 cut(s) 27
HaeIII GGCC 2 cut(s) 41, 486
HapII CCGG 2 cut(s) 347, 383
HincII GTYRAC 2 cut(s) 216, 344
HindII GTYRAC 2 cut(s) 216, 344
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 2 cut(s) 57, 370
HpaI GTTAAC 1 cut(s) 216
HpaII CCGG 2 cut(s) 347, 383
HphI GGTGA 2 cut(s) 101, 436
Hpy166II GTNNAC 2 cut(s) 216, 344
Hpy188I TCNGA 5 cut(s) 222, 272, 318, 465, 611
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 2 cut(s) 216, 344
HpyAV CCTTC 4 cut(s) 62, 71, 116, 332
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 143, 358
HpyCH4V TGCA 3 cut(s) 141, 289, 380
HpyF3I CTNAG 4 cut(s) 221, 236, 269, 315
HpySE526I ACGT 2 cut(s) 143, 358
KspAI GTTAAC 1 cut(s) 216
Kzo9I GATC 1 cut(s) 328
LguI GCTCTTC 1 cut(s) 586
LmnI GCTCC 1 cut(s) 523
MaeI CTAG 1 cut(s) 323
MaeII ACGT 2 cut(s) 143, 358
MaeIII GTNAC 1 cut(s) 431
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 3 cut(s) 47, 190, 603
MhlI GDGCHC 1 cut(s) 495
MluCI AATT 3 cut(s) 189, 453, 567
MlyI GAGTC 2 cut(s) 51, 364
MmeI TCCRAC 1 cut(s) 12
MnlI CCTC 8 cut(s) 31, 101, 104, 216, 278, 324, 514, 531
MroXI GAANNNNTTC 2 cut(s) 501, 595
MseI TTAA 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 146
MspI CCGG 2 cut(s) 347, 383
NdeI CATATG 1 cut(s) 622
NdeII GATC 1 cut(s) 328
NlaIV GGNNCC 1 cut(s) 89
PaeR7I CTCGAG 1 cut(s) 59
PceI AGGCCT 1 cut(s) 41
PciSI GCTCTTC 1 cut(s) 586
PdmI GAANNNNTTC 2 cut(s) 501, 595
PleI GAGTC 2 cut(s) 51, 364
PpsI GAGTC 2 cut(s) 51, 364
Ppu21I YACGTR 1 cut(s) 144
PpuMI RGGWCCY 1 cut(s) 88
Psp5II RGGWCCY 1 cut(s) 88
PspFI CCCAGC 1 cut(s) 23
PspN4I GGNNCC 1 cut(s) 89
PspPI GGNCC 2 cut(s) 88, 225
PspPPI RGGWCCY 1 cut(s) 88
PspXI VCTCGAGB 1 cut(s) 59
PstNI CAGNNNCTG 1 cut(s) 160
RsaI GTAC 4 cut(s) 357, 389, 420, 442
RsaNI GTAC 4 cut(s) 356, 388, 419, 441
RseI CAYNNNNRTG 1 cut(s) 146
SapI GCTCTTC 1 cut(s) 586
SaqAI TTAA 1 cut(s) 215
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 2 cut(s) 88, 225
SchI GAGTC 2 cut(s) 51, 364
SduI GDGCHC 1 cut(s) 495
Sfr274I CTCGAG 1 cut(s) 59
SgrAI CRCCGGYG 1 cut(s) 382
SinI GGWCC 2 cut(s) 88, 225
SlaI CTCGAG 1 cut(s) 59
SmiMI CAYNNNNRTG 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 59
SmoI CTYRAG 1 cut(s) 59
SpeI ACTAGT 1 cut(s) 322
Sse9I AATT 3 cut(s) 189, 453, 567
SseBI AGGCCT 1 cut(s) 41
SsiI CCGC 1 cut(s) 228
SspMI CTAG 1 cut(s) 323
StuI AGGCCT 1 cut(s) 41
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 2 cut(s) 146, 361
TaqI TCGA 5 cut(s) 12, 18, 60, 258, 495
TasI AATT 3 cut(s) 189, 453, 567
TatI WGTACW 1 cut(s) 418
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TspDTI ATGAA 3 cut(s) 81, 351, 494
VpaK11BI GGWCC 2 cut(s) 88, 225
XhoI CTCGAG 1 cut(s) 59
XmnI GAANNNNTTC 2 cut(s) 501, 595
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.