Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0004690

Physical Location & Seq

Genomic Coordinates Forward (+)

684018 .. 685259

Gene Structure 1242 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g01362.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1002 bp

ATGATGAAAAGTTCCTCCAAAATACTCTTCACCCACCTCTTCTGTGCTTTCGCAGATCTCAACCCCACCACTTACCAAGTCGCCATTTCCTCCGCAAACTCGCCTCAGTTCTCAACTTTCACTCAAACAGTCCAACAGAAAAACCCTAACGTCAAAACCCTCCTCTCCATTGGCGGAGGAAACTCCAGCCCCTCCACCTTCGCCGCCATGGCCAGCCAGGCCGCTCGTCGCAAAGCATTTATAGACTCGTCCATCAAATTAGCGAGAAACTACTCCTTCTACGGCCTTGACCTAGATTGGGAGTACCCTTCCTCCGCCACCGAGATGACCAACTTCGGCAGCCTCCTCACGGAATGGCGCTCCGCGGTGGCCAACGAGTCAAGCACCTCCGGCAAGACGGCGTTGCTGTTGACCGCTGCAGTCTTCCGGAATGCGGATTACTACAGCATAAACTATCCGTTCCAGGCTGTTTCGAGCAGCCTGGACTGGATCAACGTCATGACGTACGATTACTACGGTCCTAGTTTCGCCGAAGGTAAAACAAAAACTTACCCGCCAGCTGCATTATACAACGGCCGATCCACCACTCTCATCAACGGAGACGCCGGAATCGTGTCGTGGATCAACAACGCCGGAATCTCAACCAAGAAGATAGTCATTGGTCTTCCGTTTTATGGGTACGCTTGGAGATTGTTAAACTCTAATAACCATGCATTTTTCGCTCCGGCTAATGGCTCGGCTATCGGAGCTAACGGAGACCAGGGTTACAACCAGATTAGGGTTTTGATCAGCCGAGGGTTTCAGACGGTGTACAATGCGACGGTGGTTAGTAACTACTGTTACAATGGGACGACTTGGATTGGTTACGATGATACGCAGAGTATTGGCGCCAAGGTTTCGTATGCTAAAGGAAAGTCTCTTCTTGGTTACTTTGCTTGGCATACTGGTGCTGACACGACTACCTGGACTCTCTCTCAAACAGCTTTCAGTACATGGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0003674 GO:0003824 GO:0004553 GO:0004568 GO:0005488 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006022 GO:0006026 GO:0006030 GO:0006032 GO:0006040 GO:0006807 GO:0006810 GO:0006887 GO:0006955 GO:0007586 GO:0008061 GO:0008144 GO:0008150 GO:0008152 GO:0008843 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009617 GO:0009987 GO:0012505 GO:0016192 GO:0016787 GO:0016798 GO:0017144 GO:0030141 GO:0031410 GO:0031974 GO:0031982 GO:0031983 GO:0032501 GO:0032940 GO:0034774 GO:0035580 GO:0036230 GO:0042119 GO:0042581 GO:0042737 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043299 GO:0043312 GO:0044237 GO:0044245 GO:0044248 GO:0044421 GO:0044422 GO:0044424 GO:0044433 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046348 GO:0046903 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0060205 GO:0070013 GO:0070820 GO:0071704 GO:0097367 GO:0097708 GO:0099503 GO:1901071 GO:1901072 GO:1901135 GO:1901136 GO:1901564 GO:1901565 GO:1901575 GO:1904724

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Glyco_hydro_18

Protein Analysis

334

Amino Acids

36.32

Weight (kDa)

9.1

Isoelectric Point (pI)

28.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_18	PF00704	9 - 318	4e-71	Glycosyl hydrolases family 18

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004690)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G19720 AT4G19740 AT4G19750 AT4G19760 AT4G19770 AT4G19800 AT4G19810 AT4G19810 AT4G19820
fragaria_vesca	FvH4_1g01321 FvH4_1g01362 FvH4_1g01382
malus_domestica	MD02G1011100.v1.1 MD15G1156100.v1.1
prunus_persica	Prupe.4G180800_v2.0.a1 Prupe.7G258500_v2.0.a1 Prupe.7G259000_v2.0.a1
pyrus_communis	pycom02g01000
rosa_chinensis	RchiOBHm_Chr2g0086161
rosa_laevigata	RLG00000027969 RLG00000033389
rosa_multiflora	Rmu_co8454935.1_g000002 Rmu_sc0004510.1_g000010 Rmu_sc0004948.1_g000003 Rmu_sc0004948.1_g000021
rosa_roxburghii	Rroxscaffold_152G00434840
rosa_rugosa	Rorug01G0466300
rosa_samantha	Rh2AG016100 Rh2CG017000 Rh2DG018100
rosa_wichuraiana	Rw2G001390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	887
AccBSI	CCGCTC	1 cut(s)	224
AccII	CGCG	1 cut(s)	365
AccIII	TCCGGA	1 cut(s)	426
AclWI	GGATC	3 cut(s)	497, 573, 629
AcoI	YGGCCR	3 cut(s)	210, 369, 574
AcyI	GRCGYC	2 cut(s)	603, 888
AfaI	GTAC	5 cut(s)	305, 506, 680, 812, 991
AfiI	CCNNNNNNNGG	6 cut(s)	298, 349, 433, 674, 731, 778
AjnI	CCWGG	5 cut(s)	216, 462, 480, 759, 962
AjuI	GAANNNNNNNTTGG	2 cut(s)	11, 43
AluBI	AGCT	3 cut(s)	560, 749, 983
AluI	AGCT	3 cut(s)	560, 749, 983
Alw26I	GTCTC	3 cut(s)	594, 750, 921
AlwI	GGATC	3 cut(s)	497, 573, 629
Aor13HI	TCCGGA	1 cut(s)	426
AoxI	GGCC	5 cut(s)	210, 219, 283, 369, 574
ApeKI	GCWGC	4 cut(s)	339, 416, 477, 560
AspLEI	GCGC	2 cut(s)	360, 890
AspS9I	GGNCC	1 cut(s)	518
AsuHPI	GGTGA	1 cut(s)	22
AvaII	GGWCC	1 cut(s)	518
BalI	TGGCCA	2 cut(s)	212, 371
BanI	GGYRCC	1 cut(s)	887
BbsI	GAAGAC	2 cut(s)	415, 656
BbvI	GCAGC	4 cut(s)	351, 403, 489, 547
BccI	CCATC	1 cut(s)	260
BceAI	ACGGC	3 cut(s)	298, 414, 589
BciT130I	CCWGG	5 cut(s)	218, 464, 482, 761, 964
BclI	TGATCA	1 cut(s)	786
BcoDI	GTCTC	3 cut(s)	594, 750, 921
BfaI	CTAG	2 cut(s)	293, 522
BfmI	CTRYAG	2 cut(s)	417, 442
BfoI	RGCGCY	2 cut(s)	361, 891
BglI	GCCNNNNNGGC	2 cut(s)	209, 218
BglII	AGATCT	1 cut(s)	55
BisI	GCNGC	6 cut(s)	204, 222, 340, 417, 478, 561
BlsI	GCNGC	6 cut(s)	205, 223, 341, 418, 479, 562
Bme1390I	CCNGG	5 cut(s)	218, 464, 482, 761, 964
Bme18I	GGWCC	1 cut(s)	518
BmgT120I	GGNCC	1 cut(s)	518
BmiI	GGNNCC	1 cut(s)	889
BmrFI	CCNGG	5 cut(s)	218, 464, 482, 761, 964
BpiI	GAAGAC	2 cut(s)	415, 656
BpmI	CTGGAG	1 cut(s)	169
BsaHI	GRCGYC	2 cut(s)	603, 888
BsaI	GGTCTC	1 cut(s)	750
BsaJI	CCNNGG	5 cut(s)	207, 363, 760, 793, 891
BsaWI	WCCGGW	1 cut(s)	426
BsaXI	ACNNNNNCTCC	2 cut(s)	296, 326
Bsc4I	CCNNNNNNNGG	6 cut(s)	298, 349, 433, 674, 731, 778
Bse1I	ACTGG	2 cut(s)	491, 949
BseAI	TCCGGA	1 cut(s)	426
BseBI	CCWGG	5 cut(s)	218, 464, 482, 761, 964
BseDI	CCNNGG	5 cut(s)	207, 363, 760, 793, 891
BseLI	CCNNNNNNNGG	6 cut(s)	298, 349, 433, 674, 731, 778
BseMII	CTCAG	1 cut(s)	119
BseNI	ACTGG	2 cut(s)	491, 949
BseRI	GAGGAG	2 cut(s)	152, 335
BseX3I	CGGCCG	1 cut(s)	574
BseXI	GCAGC	4 cut(s)	351, 403, 489, 547
Bsh1236I	CGCG	1 cut(s)	365
Bsh1285I	CGRYCG	1 cut(s)	577
BshFI	GGCC	5 cut(s)	212, 221, 285, 371, 576
BshNI	GGYRCC	1 cut(s)	887
BsiEI	CGRYCG	1 cut(s)	577
BsiSI	CCGG	5 cut(s)	390, 427, 606, 633, 725
BsiWI	CGTACG	1 cut(s)	504
BslFI	GGGAC	1 cut(s)	862
BslI	CCNNNNNNNGG	6 cut(s)	298, 349, 433, 674, 731, 778
BsmAI	GTCTC	3 cut(s)	594, 750, 921
BsmBI	CGTCTC	1 cut(s)	594
BsmFI	GGGAC	1 cut(s)	862
BsmI	GAATGC	1 cut(s)	436
BsnI	GGCC	5 cut(s)	212, 221, 285, 371, 576
Bso31I	GGTCTC	1 cut(s)	750
Bsp13I	TCCGGA	1 cut(s)	426
Bsp1407I	TGTACA	1 cut(s)	810
Bsp143I	GATC	5 cut(s)	55, 489, 578, 621, 786
Bsp19I	CCATGG	1 cut(s)	207
BspANI	GGCC	5 cut(s)	212, 221, 285, 371, 576
BspCNI	CTCAG	1 cut(s)	118
BspEI	TCCGGA	1 cut(s)	426
BspFNI	CGCG	1 cut(s)	365
BspHI	TCATGA	1 cut(s)	498
BspLI	GGNNCC	1 cut(s)	889
BspMAI	CTGCAG	1 cut(s)	421
BspPI	GGATC	3 cut(s)	497, 573, 629
BspT107I	GGYRCC	1 cut(s)	887
BspTNI	GGTCTC	1 cut(s)	750
BsrBI	CCGCTC	1 cut(s)	224
BsrGI	TGTACA	1 cut(s)	810
BsrI	ACTGG	2 cut(s)	491, 949
BssECI	CCNNGG	5 cut(s)	207, 363, 760, 793, 891
BssMI	GATC	5 cut(s)	55, 489, 578, 621, 786
BssNI	GRCGYC	2 cut(s)	603, 888
BssT1I	CCWWGG	2 cut(s)	207, 891
Bst2UI	CCWGG	5 cut(s)	218, 464, 482, 761, 964
Bst4CI	ACNGT	5 cut(s)	130, 518, 808, 823, 839
Bst6I	CTCTTC	3 cut(s)	32, 44, 924
BstACI	GRCGYC	2 cut(s)	603, 888
BstAUI	TGTACA	1 cut(s)	810
BstC8I	GCNNGC	2 cut(s)	214, 558
BstDEI	CTNAG	1 cut(s)	105
BstDSI	CCRYGG	2 cut(s)	207, 363
BstFNI	CGCG	1 cut(s)	365
BstH2I	RGCGCY	2 cut(s)	361, 891
BstHHI	GCGC	2 cut(s)	360, 890
BstKTI	GATC	5 cut(s)	58, 492, 581, 624, 789
BstMAI	GTCTC	3 cut(s)	594, 750, 921
BstMBI	GATC	5 cut(s)	55, 489, 578, 621, 786
BstMCI	CGRYCG	1 cut(s)	577
BstMWI	GCNNNNNNNGC	5 cut(s)	209, 218, 390, 719, 746
BstNI	CCWGG	5 cut(s)	218, 464, 482, 761, 964
BstSCI	CCNGG	5 cut(s)	216, 462, 480, 759, 962
BstSFI	CTRYAG	2 cut(s)	417, 442
BstUI	CGCG	1 cut(s)	365
BstV1I	GCAGC	4 cut(s)	351, 403, 489, 547
BstV2I	GAAGAC	2 cut(s)	415, 656
BstX2I	RGATCY	1 cut(s)	55
BstYI	RGATCY	1 cut(s)	55
BstZI	CGGCCG	1 cut(s)	574
BsuRI	GGCC	5 cut(s)	212, 221, 285, 371, 576
BtgI	CCRYGG	2 cut(s)	207, 363
Cac8I	GCNNGC	2 cut(s)	214, 558
CciI	TCATGA	1 cut(s)	498
CfoI	GCGC	2 cut(s)	360, 890
Cfr13I	GGNCC	1 cut(s)	518
Cfr42I	CCGCGG	1 cut(s)	366
CseI	GACGC	1 cut(s)	611
Csp6I	GTAC	5 cut(s)	304, 505, 679, 811, 990
CviAII	CATG	4 cut(s)	208, 499, 710, 993
CviQI	GTAC	5 cut(s)	304, 505, 679, 811, 990
DdeI	CTNAG	1 cut(s)	105
DinI	GGCGCC	1 cut(s)	889
DpnI	GATC	5 cut(s)	57, 491, 580, 623, 788
DpnII	GATC	5 cut(s)	55, 489, 578, 621, 786
EaeI	YGGCCR	3 cut(s)	210, 369, 574
EagI	CGGCCG	1 cut(s)	574
Eam1104I	CTCTTC	3 cut(s)	32, 44, 924
EarI	CTCTTC	3 cut(s)	32, 44, 924
EciI	GGCGGA	2 cut(s)	189, 304
EclXI	CGGCCG	1 cut(s)	574
Eco130I	CCWWGG	2 cut(s)	207, 891
Eco31I	GGTCTC	1 cut(s)	750
Eco47I	GGWCC	1 cut(s)	518
Eco52I	CGGCCG	1 cut(s)	574
EcoRII	CCWGG	5 cut(s)	216, 462, 480, 759, 962
EcoT14I	CCWWGG	2 cut(s)	207, 891
EcoT22I	ATGCAT	1 cut(s)	715
EgeI	GGCGCC	1 cut(s)	889
EheI	GGCGCC	1 cut(s)	889
ErhI	CCWWGG	2 cut(s)	207, 891
Esp3I	CGTCTC	1 cut(s)	594
FaeI	CATG	4 cut(s)	211, 502, 713, 996
FaqI	GGGAC	1 cut(s)	862
FatI	CATG	4 cut(s)	207, 498, 709, 992
FauI	CCCGC	1 cut(s)	561
FbaI	TGATCA	1 cut(s)	786
Fnu4HI	GCNGC	6 cut(s)	204, 222, 340, 417, 478, 561
Fsp4HI	GCNGC	6 cut(s)	204, 222, 340, 417, 478, 561
FspBI	CTAG	2 cut(s)	293, 522
GlaI	GCGC	2 cut(s)	359, 889
GluI	GCNGC	6 cut(s)	204, 222, 340, 417, 478, 561
GsuI	CTGGAG	1 cut(s)	169
HaeII	RGCGCY	2 cut(s)	361, 891
HaeIII	GGCC	5 cut(s)	212, 221, 285, 371, 576
HapII	CCGG	5 cut(s)	390, 427, 606, 633, 725
HgaI	GACGC	1 cut(s)	611
HhaI	GCGC	2 cut(s)	360, 890
Hin1I	GRCGYC	2 cut(s)	603, 888
Hin1II	CATG	4 cut(s)	211, 502, 713, 996
Hin6I	GCGC	2 cut(s)	358, 888
HinP1I	GCGC	2 cut(s)	358, 888
HincII	GTYRAC	1 cut(s)	411
HindII	GTYRAC	1 cut(s)	411
HinfI	GANTC	5 cut(s)	245, 377, 609, 636, 967
HpaII	CCGG	5 cut(s)	390, 427, 606, 633, 725
HphI	GGTGA	1 cut(s)	22
Hpy166II	GTNNAC	2 cut(s)	411, 811
Hpy188I	TCNGA	2 cut(s)	746, 804
Hpy188III	TCNNGA	2 cut(s)	427, 499
Hpy8I	GTNNAC	2 cut(s)	411, 811
Hpy99I	CGWCG	2 cut(s)	231, 823
HpyAV	CCTTC	4 cut(s)	208, 286, 318, 527
HpyCH4III	ACNGT	5 cut(s)	130, 518, 808, 823, 839
HpyCH4IV	ACGT	3 cut(s)	150, 495, 503
HpyCH4V	TGCA	3 cut(s)	419, 563, 713
HpyF10VI	GCNNNNNNNGC	5 cut(s)	209, 218, 390, 719, 746
HpyF3I	CTNAG	1 cut(s)	105
HpySE526I	ACGT	3 cut(s)	150, 495, 503
Hsp92I	GRCGYC	2 cut(s)	603, 888
Hsp92II	CATG	4 cut(s)	211, 502, 713, 996
HspAI	GCGC	2 cut(s)	358, 888
KasI	GGCGCC	1 cut(s)	887
Kpn2I	TCCGGA	1 cut(s)	426
Ksp22I	TGATCA	1 cut(s)	786
KspI	CCGCGG	1 cut(s)	366
Kzo9I	GATC	5 cut(s)	55, 489, 578, 621, 786
LmnI	GCTCC	3 cut(s)	365, 727, 746
Lsp1109I	GCAGC	4 cut(s)	351, 403, 489, 547
MaeI	CTAG	2 cut(s)	293, 522
MaeII	ACGT	3 cut(s)	150, 495, 503
MaeIII	GTNAC	5 cut(s)	764, 830, 839, 863, 926
MalI	GATC	5 cut(s)	57, 491, 580, 623, 788
MbiI	CCGCTC	1 cut(s)	224
MboI	GATC	5 cut(s)	55, 489, 578, 621, 786
MboII	GAAGA	6 cut(s)	19, 31, 415, 656, 661, 911
MflI	RGATCY	1 cut(s)	55
MlsI	TGGCCA	2 cut(s)	212, 371
MluCI	AATT	1 cut(s)	257
MluNI	TGGCCA	2 cut(s)	212, 371
Mly113I	GGCGCC	1 cut(s)	888
MlyI	GAGTC	3 cut(s)	239, 386, 961
MmeI	TCCRAC	1 cut(s)	157
Mox20I	TGGCCA	2 cut(s)	212, 371
Mph1103I	ATGCAT	1 cut(s)	715
MroI	TCCGGA	1 cut(s)	426
MscI	TGGCCA	2 cut(s)	212, 371
MseI	TTAA	1 cut(s)	695
MslI	CAYNNNNRTG	3 cut(s)	323, 945, 997
Msp20I	TGGCCA	2 cut(s)	212, 371
MspA1I	CMGCKG	3 cut(s)	365, 416, 560
MspI	CCGG	5 cut(s)	390, 427, 606, 633, 725
MspR9I	CCNGG	5 cut(s)	218, 464, 482, 761, 964
Mva1269I	GAATGC	1 cut(s)	436
MvaI	CCWGG	5 cut(s)	218, 464, 482, 761, 964
MvnI	CGCG	1 cut(s)	365
MwoI	GCNNNNNNNGC	5 cut(s)	209, 218, 390, 719, 746
NarI	GGCGCC	1 cut(s)	888
NcoI	CCATGG	1 cut(s)	207
NdeII	GATC	5 cut(s)	55, 489, 578, 621, 786
NlaIII	CATG	4 cut(s)	211, 502, 713, 996
NlaIV	GGNNCC	1 cut(s)	889
NmeAIII	GCCGAG	2 cut(s)	716, 818
NsiI	ATGCAT	1 cut(s)	715
PagI	TCATGA	1 cut(s)	498
PcsI	WCGNNNNNNNCGW	1 cut(s)	609
PctI	GAATGC	1 cut(s)	436
PfeI	GAWTC	2 cut(s)	609, 636
Pfl23II	CGTACG	1 cut(s)	504
PkrI	GCNGC	6 cut(s)	205, 223, 341, 418, 479, 562
PleI	GAGTC	3 cut(s)	239, 385, 961
PluTI	GGCGCC	1 cut(s)	891
PpsI	GAGTC	3 cut(s)	239, 385, 961
Psp6I	CCWGG	5 cut(s)	216, 462, 480, 759, 962
PspGI	CCWGG	5 cut(s)	216, 462, 480, 759, 962
PspLI	CGTACG	1 cut(s)	504
PspN4I	GGNNCC	1 cut(s)	889
PspPI	GGNCC	1 cut(s)	518
PstI	CTGCAG	1 cut(s)	421
PsuI	RGATCY	1 cut(s)	55
PvuII	CAGCTG	1 cut(s)	560
RsaI	GTAC	5 cut(s)	305, 506, 680, 812, 991
RsaNI	GTAC	5 cut(s)	304, 505, 679, 811, 990
RseI	CAYNNNNRTG	3 cut(s)	323, 945, 997
SacII	CCGCGG	1 cut(s)	366
SaqAI	TTAA	1 cut(s)	695
SatI	GCNGC	6 cut(s)	204, 222, 340, 417, 478, 561
Sau3AI	GATC	5 cut(s)	55, 489, 578, 621, 786
Sau96I	GGNCC	1 cut(s)	518
SchI	GAGTC	3 cut(s)	239, 386, 961
ScrFI	CCNGG	5 cut(s)	218, 464, 482, 761, 964
SfcI	CTRYAG	2 cut(s)	417, 442
SfiI	GGCCNNNNNGGCC	1 cut(s)	218
SfoI	GGCGCC	1 cut(s)	889
Sfr303I	CCGCGG	1 cut(s)	366
SgrBI	CCGCGG	1 cut(s)	366
SinI	GGWCC	1 cut(s)	518
SmiMI	CAYNNNNRTG	3 cut(s)	323, 945, 997
Sse9I	AATT	1 cut(s)	257
SspDI	GGCGCC	1 cut(s)	887
SspMI	CTAG	2 cut(s)	293, 522
StyD4I	CCNGG	5 cut(s)	216, 462, 480, 759, 962
StyI	CCWWGG	2 cut(s)	207, 891
TaaI	ACNGT	5 cut(s)	130, 518, 808, 823, 839
TaiI	ACGT	3 cut(s)	153, 498, 506
TaqI	TCGA	1 cut(s)	473
TasI	AATT	1 cut(s)	257
TatI	WGTACW	2 cut(s)	810, 989
TauI	GCSGC	2 cut(s)	206, 224
TfiI	GAWTC	2 cut(s)	609, 636
Tru1I	TTAA	1 cut(s)	695
Tru9I	TTAA	1 cut(s)	695
TseI	GCWGC	4 cut(s)	339, 416, 477, 560
TspDTI	ATGAA	1 cut(s)	20
TspGWI	ACGGA	5 cut(s)	365, 447, 612, 657, 768
VpaK11BI	GGWCC	1 cut(s)	518
XspI	CTAG	2 cut(s)	293, 522
Zsp2I	ATGCAT	1 cut(s)	715

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.