Rroxscaffold_152G00434840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000152
OG0004690
Physical Location & Seq
Reverse (-)
380312 .. 380623
312 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_152G00434840.1

Sequence Viewer

Length: 312 bp
ATGCTGCTCCAGCACGATTCCGACCACCTTCGTCTTCCCCATGCCCTCTTCTCCGCCGCACCGGTCCCCGGCCCGGCGCGACCTCATCCATGCTGCGCAGTGCCCCGTGCGGCGCTCTCCACCGTCACGGCCACCATCTCTCGACGTCTCCGCATCGAGGAAACCGGCGATTCACCGAGATCCGACATCCTCATCTGCACCACCGGCGATTCACCACCACCGCAAAGAATCGGCTCCGGCTCGGCTGGATGCGCACCTCCACATCACGGTGCCGCAACCGCTTTCGGGACGGGCGGGGAGTCGGCGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

10.55

Weight (kDa)

7.78

Isoelectric Point (pI)

71.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 148
Acc16I TGCGCA 2 cut(s) 97, 253
AccB1I GGYRCC 1 cut(s) 269
AccII CGCG 1 cut(s) 79
AciI CCGC 8 cut(s) 54, 57, 110, 151, 221, 273, 279, 294
AclWI GGATC 1 cut(s) 174
AcoI YGGCCR 1 cut(s) 129
AcyI GRCGYC 1 cut(s) 145
AfiI CCNNNNNNNGG 5 cut(s) 68, 73, 157, 266, 285
AgeI ACCGGT 1 cut(s) 61
Alw26I GTCTC 1 cut(s) 152
AlwI GGATC 1 cut(s) 174
AoxI GGCC 2 cut(s) 70, 129
ApeKI GCWGC 2 cut(s) 4, 93
AsiGI ACCGGT 1 cut(s) 61
AspLEI GCGC 4 cut(s) 79, 98, 115, 254
AspS9I GGNCC 2 cut(s) 64, 71
AsuC2I CCSGG 2 cut(s) 69, 74
AsuHPI GGTGA 2 cut(s) 165, 204
AvaII GGWCC 1 cut(s) 64
BaeGI GKGCMC 1 cut(s) 105
BanI GGYRCC 1 cut(s) 269
BbsI GAAGAC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 80
BccI CCATC 1 cut(s) 143
BceAI ACGGC 1 cut(s) 144
BcnI CCSGG 2 cut(s) 69, 74
BcoDI GTCTC 1 cut(s) 152
BfoI RGCGCY 1 cut(s) 116
BisI GCNGC 5 cut(s) 5, 57, 94, 111, 273
BlsI GCNGC 5 cut(s) 6, 58, 95, 112, 274
Bme1390I CCNGG 2 cut(s) 69, 74
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 64, 71
BmiI GGNNCC 3 cut(s) 66, 235, 271
BmrFI CCNGG 2 cut(s) 69, 74
BmsI GCATC 2 cut(s) 162, 239
BpiI GAAGAC 1 cut(s) 26
BpuMI CCSGG 2 cut(s) 69, 74
BsaHI GRCGYC 1 cut(s) 145
BsaJI CCNNGG 1 cut(s) 67
BsaWI WCCGGW 1 cut(s) 61
Bsc4I CCNNNNNNNGG 5 cut(s) 68, 73, 157, 266, 285
Bse118I RCCGGY 3 cut(s) 61, 164, 203
BseDI CCNNGG 1 cut(s) 67
BseGI GGATG 3 cut(s) 85, 186, 254
BseLI CCNNNNNNNGG 5 cut(s) 68, 73, 157, 266, 285
BseSI GKGCMC 1 cut(s) 105
BseXI GCAGC 1 cut(s) 80
BsgI GTGCAG 1 cut(s) 181
Bsh1236I CGCG 1 cut(s) 79
BshFI GGCC 2 cut(s) 72, 131
BshNI GGYRCC 1 cut(s) 269
BshTI ACCGGT 1 cut(s) 61
BsiSI CCGG 6 cut(s) 62, 69, 74, 165, 204, 237
BslFI GGGAC 2 cut(s) 50, 301
BslI CCNNNNNNNGG 5 cut(s) 68, 73, 157, 266, 285
BsmAI GTCTC 1 cut(s) 152
BsmBI CGTCTC 1 cut(s) 152
BsmFI GGGAC 2 cut(s) 50, 301
BsnI GGCC 2 cut(s) 72, 131
Bsp1286I GDGCHC 1 cut(s) 105
Bsp143I GATC 1 cut(s) 179
BspACI CCGC 8 cut(s) 54, 57, 110, 151, 221, 273, 279, 294
BspANI GGCC 2 cut(s) 72, 131
BspFNI CGCG 1 cut(s) 79
BspLI GGNNCC 3 cut(s) 66, 235, 271
BspPI GGATC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 269
BsrFI RCCGGY 3 cut(s) 61, 164, 203
BssAI RCCGGY 3 cut(s) 61, 164, 203
BssECI CCNNGG 1 cut(s) 67
BssMI GATC 1 cut(s) 179
BssNI GRCGYC 1 cut(s) 145
Bst4CI ACNGT 2 cut(s) 124, 269
Bst6I CTCTTC 1 cut(s) 53
BstACI GRCGYC 1 cut(s) 145
BstF5I GGATG 3 cut(s) 85, 186, 254
BstFNI CGCG 1 cut(s) 79
BstH2I RGCGCY 1 cut(s) 116
BstHHI GCGC 4 cut(s) 79, 98, 115, 254
BstKTI GATC 1 cut(s) 182
BstMAI GTCTC 1 cut(s) 152
BstMBI GATC 1 cut(s) 179
BstMWI GCNNNNNNNGC 4 cut(s) 10, 204, 251, 278
BstSCI CCNGG 2 cut(s) 67, 72
BstSLI GKGCMC 1 cut(s) 105
BstUI CGCG 1 cut(s) 79
BstV1I GCAGC 1 cut(s) 80
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 179
BstYI RGATCY 1 cut(s) 179
BsuRI GGCC 2 cut(s) 72, 131
BtsCI GGATG 3 cut(s) 85, 186, 254
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 105
CfoI GCGC 4 cut(s) 79, 98, 115, 254
Cfr10I RCCGGY 3 cut(s) 61, 164, 203
Cfr13I GGNCC 2 cut(s) 64, 71
CspAI ACCGGT 1 cut(s) 61
CviAII CATG 2 cut(s) 41, 90
CviJI RGCY 5 cut(s) 72, 131, 234, 240, 245
CviKI_1 RGCY 5 cut(s) 72, 131, 234, 240, 245
DpnI GATC 1 cut(s) 181
DpnII GATC 1 cut(s) 179
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
EciI GGCGGA 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 64
Esp3I CGTCTC 1 cut(s) 152
FaeI CATG 2 cut(s) 44, 93
FaiI YATR 2 cut(s) 42, 91
FaqI GGGAC 2 cut(s) 50, 301
FatI CATG 2 cut(s) 40, 89
FauI CCCGC 1 cut(s) 287
Fnu4HI GCNGC 5 cut(s) 5, 57, 94, 111, 273
FokI GGATG 3 cut(s) 72, 173, 261
Fsp4HI GCNGC 5 cut(s) 5, 57, 94, 111, 273
FspAI RTGCGCAY 1 cut(s) 253
FspI TGCGCA 2 cut(s) 97, 253
GlaI GCGC 4 cut(s) 78, 97, 114, 253
GluI GCNGC 5 cut(s) 5, 57, 94, 111, 273
HaeII RGCGCY 1 cut(s) 116
HaeIII GGCC 2 cut(s) 72, 131
HapII CCGG 6 cut(s) 62, 69, 74, 165, 204, 237
HhaI GCGC 4 cut(s) 79, 98, 115, 254
Hin1I GRCGYC 1 cut(s) 145
Hin1II CATG 2 cut(s) 44, 93
Hin6I GCGC 4 cut(s) 77, 96, 113, 252
HinP1I GCGC 4 cut(s) 77, 96, 113, 252
HinfI GANTC 5 cut(s) 17, 170, 209, 228, 299
HpaII CCGG 6 cut(s) 62, 69, 74, 165, 204, 237
HphI GGTGA 2 cut(s) 165, 204
Hpy188I TCNGA 2 cut(s) 22, 184
Hpy188III TCNNGA 2 cut(s) 141, 286
Hpy99I CGWCG 1 cut(s) 147
HpyAV CCTTC 1 cut(s) 38
HpyCH4III ACNGT 2 cut(s) 124, 269
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 1 cut(s) 198
HpyF10VI GCNNNNNNNGC 4 cut(s) 10, 204, 251, 278
HpySE526I ACGT 1 cut(s) 145
Hsp92I GRCGYC 1 cut(s) 145
Hsp92II CATG 2 cut(s) 44, 93
HspAI GCGC 4 cut(s) 77, 96, 113, 252
Kzo9I GATC 1 cut(s) 179
LmnI GCTCC 2 cut(s) 12, 239
LpnPI CCDG 8 cut(s) 23, 75, 82, 87, 178, 217, 231, 250
Lsp1109I GCAGC 1 cut(s) 80
LweI GCATC 2 cut(s) 162, 239
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 1 cut(s) 124
MalI GATC 1 cut(s) 181
MboI GATC 1 cut(s) 179
MboII GAAGA 2 cut(s) 26, 40
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 1 cut(s) 105
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 2 cut(s) 45, 207
MnlI CCTC 5 cut(s) 56, 93, 151, 200, 267
MslI CAYNNNNRTG 1 cut(s) 267
MspI CCGG 6 cut(s) 62, 69, 74, 165, 204, 237
MspR9I CCNGG 2 cut(s) 69, 74
MvnI CGCG 1 cut(s) 79
MwoI GCNNNNNNNGC 4 cut(s) 10, 204, 251, 278
NciI CCSGG 2 cut(s) 69, 74
NdeII GATC 1 cut(s) 179
NlaIII CATG 2 cut(s) 44, 93
NlaIV GGNNCC 3 cut(s) 66, 235, 271
NmeAIII GCCGAG 1 cut(s) 221
NmuCI GTSAC 1 cut(s) 124
NsbI TGCGCA 2 cut(s) 97, 253
PfeI GAWTC 4 cut(s) 17, 170, 209, 228
PinAI ACCGGT 1 cut(s) 61
PkrI GCNGC 5 cut(s) 6, 58, 95, 112, 274
PleI GAGTC 1 cut(s) 307
PpsI GAGTC 1 cut(s) 307
PspN4I GGNNCC 3 cut(s) 66, 235, 271
PspPI GGNCC 2 cut(s) 64, 71
PsuI RGATCY 1 cut(s) 179
RseI CAYNNNNRTG 1 cut(s) 267
SatI GCNGC 5 cut(s) 5, 57, 94, 111, 273
Sau3AI GATC 1 cut(s) 179
Sau96I GGNCC 2 cut(s) 64, 71
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 2 cut(s) 69, 74
SduI GDGCHC 1 cut(s) 105
SetI ASST 4 cut(s) 30, 85, 148, 259
SfaNI GCATC 2 cut(s) 162, 239
SgrAI CRCCGGYG 1 cut(s) 203
SinI GGWCC 1 cut(s) 64
SmiMI CAYNNNNRTG 1 cut(s) 267
SsiI CCGC 8 cut(s) 54, 57, 110, 151, 221, 273, 279, 294
StyD4I CCNGG 2 cut(s) 67, 72
TaaI ACNGT 2 cut(s) 124, 269
TaiI ACGT 1 cut(s) 148
TaqI TCGA 2 cut(s) 142, 156
TauI GCSGC 3 cut(s) 59, 113, 275
TfiI GAWTC 4 cut(s) 17, 170, 209, 228
TscAI CASTG 1 cut(s) 105
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 2 cut(s) 4, 93
Tsp45I GTSAC 1 cut(s) 124
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 64
ZraI GACGTC 1 cut(s) 146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.