FvH4_1g01891

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002821
Physical Location & Seq
Forward (+)
987413 .. 990608
3196 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01891.t1

Sequence Viewer

Length: 1014 bp
ATGGCCACGACGGCTCCCTCCTCCCTCCACCAAACATTTCTATCCCCAAAACACCCTCGCCTCCACCACAATTCCCATCTCTCTCCTCCTCGTCTCCGCTTCCACTCTCTCCCCATTCGCGCCACTACAATCACTCTACAACAGGAGCAGAGCTCAAGCACCCAGAAAAGTCAAAGCTCAGGCATTGAGTTTCTGTCATGCCCAGTTTGCTATGAACCCCTCATACGCAAAGGCCCAACTGGTTTGAACTTGGAAGCAATATACAGGTCAGGTTTCAAGTGCAGGAAATGCGACAAGTCGTATTCCAGCAAGGACATTTACTTGGACCTTACTGTTACTGCTGGGTTGAAGCAGTATGTTGAGGTCAACCCTGCTAGGACTGAGTTGTTTAGGAGCCCTCTTGTGTCGTTTTTGTATGAGAGAGGTTGGCGCCAGAATTTTAGTAGTAGTGGATTTCCAGGTCCTGATGAAGAGTTTAAAATGGCTCAGGACTACTTTAAACCCGCGGAAGGCGGTCTTCTGGTGGATGTGAGCTGTGGGAGTGGTTTGTTTTCAAGGAAATTTGCAAAATCGGGGTCTTATTCTGGAGTTGTGGCCCTTGATTTTTCAGAAAATATGCTTAGACAGTGCTATGATTTCCTCAAAAGAGATGCTACTCTTTTGGCTACGAATCTTGCTCTTGTAAGGGCAGATGTTTCGAGGCTCCCATTTGCATCAGGTTCAGTCGATGCTGTCCATGCCGGTGCAGCACTGCATTGCTGGCCCTCTCCTTCAAATGCTGTTGCTGAAATTACTCGTGTACTACGAAGTGGCGGTGTCTTTGTTGGAACCACTTTTCTGCGGTATACTCCAACCACTCCCTGGTATATCAGGCCTTTCAGAGAGAGGATTGCTCAGGGCTACAGCTACTTAACGGAGGAAGAGATACAGGACTTGTGTACATCATGTGGCCTCACGAACTACTCGAGCAAAGTTCAGCAATCCTTCATCATGTTTTCAGCTCAGAAACCTTGA

Protein Analysis

338

Amino Acids

37.54

Weight (kDa)

9.08

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 166 - 279 1.3e-11 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 168 - 322 1.1e-07 Methyltransferase domain
Methyltransf_31 PF13847 174 - 279 2.9e-12 Methyltransferase domain
Methyltransf_11 PF08241 175 - 275 5.9e-21 Methyltransferase domain
Methyltransf_25 PF13649 175 - 272 2.8e-17 Methyltransferase domain
Methyltransf_12 PF08242 175 - 274 1.8e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 429
AccB7I CCANNNNNTGG 1 cut(s) 861
AccI GTMKAC 1 cut(s) 845
AccII CGCG 2 cut(s) 120, 506
AciI CCGC 6 cut(s) 97, 504, 506, 513, 813, 841
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 436, 560
AcyI GRCGYC 1 cut(s) 430
AfaI GTAC 2 cut(s) 801, 940
AfiI CCNNNNNNNGG 2 cut(s) 509, 861
AgsI TTSAA 5 cut(s) 247, 277, 349, 555, 774
AjnI CCWGG 2 cut(s) 457, 860
AluBI AGCT 5 cut(s) 153, 177, 534, 906, 1001
AluI AGCT 5 cut(s) 153, 177, 534, 906, 1001
Alw21I GWGCWC 1 cut(s) 155
Alw26I GTCTC 1 cut(s) 98
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 964
AoxI GGCC 6 cut(s) 3, 232, 594, 761, 872, 949
ApeKI GCWGC 1 cut(s) 746
ApoI RAATTY 2 cut(s) 436, 560
ArsI GACNNNNNNTTYG 2 cut(s) 560, 592
AspLEI GCGC 2 cut(s) 122, 432
AspS9I GGNCC 5 cut(s) 233, 325, 461, 595, 762
AvaI CYCGRG 1 cut(s) 964
AvaII GGWCC 2 cut(s) 325, 461
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 429
BanII GRGCYC 2 cut(s) 155, 398
BauI CACGAG 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 155
BbvI GCAGC 1 cut(s) 758
BccI CCATC 1 cut(s) 84
BceAI ACGGC 1 cut(s) 27
BcgI CGANNNNNNTGC 2 cut(s) 678, 712
BciT130I CCWGG 2 cut(s) 459, 862
BcoDI GTCTC 1 cut(s) 98
BfaI CTAG 1 cut(s) 375
BfmI CTRYAG 1 cut(s) 901
BfoI RGCGCY 1 cut(s) 433
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 1 cut(s) 747
BlsI GCNGC 1 cut(s) 748
Bme1390I CCNGG 2 cut(s) 459, 862
Bme18I GGWCC 2 cut(s) 325, 461
BmeT110I CYCGRG 1 cut(s) 964
BmgT120I GGNCC 5 cut(s) 233, 325, 461, 595, 762
BmiI GGNNCC 5 cut(s) 15, 395, 431, 704, 829
BmrFI CCNGG 2 cut(s) 459, 862
BmrI ACTGGG 1 cut(s) 197
BmsI GCATC 3 cut(s) 640, 718, 722
BmuI ACTGGG 1 cut(s) 197
BpiI GAAGAC 1 cut(s) 509
BplI GAGNNNNNCTC 4 cut(s) 137, 169, 877, 909
BpmI CTGGAG 1 cut(s) 606
Bpu10I CCTNAGC 3 cut(s) 178, 486, 894
BpuEI CTTGAG 1 cut(s) 139
BsaHI GRCGYC 1 cut(s) 430
BsaJI CCNNGG 2 cut(s) 504, 860
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 2 cut(s) 509, 861
Bse118I RCCGGY 1 cut(s) 740
Bse1I ACTGG 2 cut(s) 203, 244
Bse3DI GCAATG 1 cut(s) 754
BseBI CCWGG 2 cut(s) 459, 862
BseDI CCNNGG 2 cut(s) 504, 860
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 2 cut(s) 509, 861
BseMI GCAATG 1 cut(s) 754
BseMII CTCAG 4 cut(s) 192, 372, 500, 908
BseNI ACTGG 2 cut(s) 203, 244
BseRI GAGGAG 3 cut(s) 10, 75, 78
BseXI GCAGC 1 cut(s) 758
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 2 cut(s) 301, 765
Bsh1236I CGCG 2 cut(s) 120, 506
BshFI GGCC 6 cut(s) 5, 234, 596, 763, 874, 951
BshNI GGYRCC 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 155
BsiHKCI CYCGRG 1 cut(s) 964
BsiSI CCGG 1 cut(s) 741
BslI CCNNNNNNNGG 2 cut(s) 509, 861
BsmAI GTCTC 1 cut(s) 98
BsmBI CGTCTC 1 cut(s) 98
BsnI GGCC 6 cut(s) 5, 234, 596, 763, 874, 951
BsoBI CYCGRG 1 cut(s) 964
Bsp1286I GDGCHC 2 cut(s) 155, 398
Bsp1407I TGTACA 1 cut(s) 938
BspACI CCGC 6 cut(s) 97, 504, 506, 513, 813, 841
BspANI GGCC 6 cut(s) 5, 234, 596, 763, 874, 951
BspCNI CTCAG 4 cut(s) 191, 373, 499, 907
BspFNI CGCG 2 cut(s) 120, 506
BspLI GGNNCC 5 cut(s) 15, 395, 431, 704, 829
BspT107I GGYRCC 1 cut(s) 429
BsrDI GCAATG 1 cut(s) 754
BsrFI RCCGGY 1 cut(s) 740
BsrGI TGTACA 1 cut(s) 938
BsrI ACTGG 2 cut(s) 203, 244
BssAI RCCGGY 1 cut(s) 740
BssECI CCNNGG 2 cut(s) 504, 860
BssNAI GTATAC 1 cut(s) 846
BssNI GRCGYC 1 cut(s) 430
BssSI CACGAG 1 cut(s) 795
Bst1107I GTATAC 1 cut(s) 846
Bst2BI CACGAG 1 cut(s) 795
Bst2UI CCWGG 2 cut(s) 459, 862
Bst4CI ACNGT 2 cut(s) 334, 627
Bst6I CTCTTC 2 cut(s) 465, 915
BstACI GRCGYC 1 cut(s) 430
BstAPI GCANNNNNTGC 1 cut(s) 288
BstAUI TGTACA 1 cut(s) 938
BstC8I GCNNGC 1 cut(s) 761
BstDEI CTNAG 6 cut(s) 178, 381, 486, 620, 894, 1002
BstDSI CCRYGG 1 cut(s) 504
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 2 cut(s) 120, 506
BstH2I RGCGCY 1 cut(s) 433
BstHHI GCGC 2 cut(s) 122, 432
BstMAI GTCTC 1 cut(s) 98
BstMWI GCNNNNNNNGC 6 cut(s) 11, 207, 288, 737, 746, 760
BstNI CCWGG 2 cut(s) 459, 862
BstSCI CCNGG 2 cut(s) 457, 860
BstSFI CTRYAG 1 cut(s) 901
BstUI CGCG 2 cut(s) 120, 506
BstV1I GCAGC 1 cut(s) 758
BstV2I GAAGAC 1 cut(s) 509
BstZ17I GTATAC 1 cut(s) 846
BsuRI GGCC 6 cut(s) 5, 234, 596, 763, 874, 951
BtgI CCRYGG 1 cut(s) 504
BtsCI GGATG 1 cut(s) 532
BtsI GCAGTG 1 cut(s) 749
BtsIMutI CAGTG 2 cut(s) 632, 749
Cac8I GCNNGC 1 cut(s) 761
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 2 cut(s) 122, 432
Cfr10I RCCGGY 1 cut(s) 740
Cfr13I GGNCC 5 cut(s) 233, 325, 461, 595, 762
Cfr42I CCGCGG 1 cut(s) 507
Csp6I GTAC 2 cut(s) 800, 939
CviAII CATG 4 cut(s) 198, 737, 945, 991
CviQI GTAC 2 cut(s) 800, 939
DdeI CTNAG 6 cut(s) 178, 381, 486, 620, 894, 1002
DinI GGCGCC 1 cut(s) 431
DraI TTTAAA 2 cut(s) 478, 499
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 465, 915
EarI CTCTTC 2 cut(s) 465, 915
Ecl136II GAGCTC 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 874
Eco24I GRGCYC 2 cut(s) 155, 398
Eco47I GGWCC 2 cut(s) 325, 461
Eco53kI GAGCTC 1 cut(s) 153
Eco88I CYCGRG 1 cut(s) 964
EcoICRI GAGCTC 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 461
EcoRII CCWGG 2 cut(s) 457, 860
EcoT38I GRGCYC 2 cut(s) 155, 398
EgeI GGCGCC 1 cut(s) 431
EheI GGCGCC 1 cut(s) 431
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 4 cut(s) 201, 740, 948, 994
FalI AAGNNNNNCTT 2 cut(s) 501, 533
FatI CATG 4 cut(s) 197, 736, 944, 990
FauI CCCGC 1 cut(s) 511
FblI GTMKAC 1 cut(s) 845
Fnu4HI GCNGC 1 cut(s) 747
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 2 cut(s) 155, 398
Fsp4HI GCNGC 1 cut(s) 747
FspBI CTAG 1 cut(s) 375
GlaI GCGC 2 cut(s) 121, 431
GluI GCNGC 1 cut(s) 747
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 606
HaeII RGCGCY 1 cut(s) 433
HaeIII GGCC 6 cut(s) 5, 234, 596, 763, 874, 951
HapII CCGG 1 cut(s) 741
HhaI GCGC 2 cut(s) 122, 432
Hin1I GRCGYC 1 cut(s) 430
Hin1II CATG 4 cut(s) 201, 740, 948, 994
Hin6I GCGC 2 cut(s) 120, 430
HinP1I GCGC 2 cut(s) 120, 430
HincII GTYRAC 1 cut(s) 367
HindII GTYRAC 1 cut(s) 367
HinfI GANTC 1 cut(s) 670
HpaII CCGG 1 cut(s) 741
Hpy166II GTNNAC 4 cut(s) 367, 800, 846, 939
Hpy188I TCNGA 3 cut(s) 610, 881, 1005
Hpy188III TCNNGA 4 cut(s) 464, 488, 585, 955
Hpy8I GTNNAC 4 cut(s) 367, 800, 846, 939
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 3 cut(s) 503, 780, 994
HpyCH4III ACNGT 2 cut(s) 334, 627
HpyCH4V TGCA 5 cut(s) 282, 566, 713, 746, 754
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 207, 288, 737, 746, 760
HpyF3I CTNAG 6 cut(s) 178, 381, 486, 620, 894, 1002
Hsp92I GRCGYC 1 cut(s) 430
Hsp92II CATG 4 cut(s) 201, 740, 948, 994
HspAI GCGC 2 cut(s) 120, 430
KasI GGCGCC 1 cut(s) 429
KspI CCGCGG 1 cut(s) 507
LmnI GCTCC 4 cut(s) 19, 145, 393, 708
Lsp1109I GCAGC 1 cut(s) 758
LweI GCATC 3 cut(s) 640, 718, 722
MaeI CTAG 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 334
MboII GAAGA 3 cut(s) 482, 509, 932
MhlI GDGCHC 2 cut(s) 155, 398
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 70, 436, 560, 789
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 430
MmeI TCCRAC 2 cut(s) 805, 875
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 477, 498, 911
MslI CAYNNNNRTG 1 cut(s) 741
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 1 cut(s) 741
MspR9I CCNGG 2 cut(s) 459, 862
MvaI CCWGG 2 cut(s) 459, 862
MvnI CGCG 2 cut(s) 120, 506
MwoI GCNNNNNNNGC 6 cut(s) 11, 207, 288, 737, 746, 760
NarI GGCGCC 1 cut(s) 430
NlaIII CATG 4 cut(s) 201, 740, 948, 994
NlaIV GGNNCC 5 cut(s) 15, 395, 431, 704, 829
PaeR7I CTCGAG 1 cut(s) 964
PceI AGGCCT 1 cut(s) 874
PcsI WCGNNNNNNNCGW 2 cut(s) 802, 962
PfeI GAWTC 1 cut(s) 670
PflMI CCANNNNNTGG 1 cut(s) 861
PkrI GCNGC 1 cut(s) 748
PluTI GGCGCC 1 cut(s) 433
PpuMI RGGWCCY 1 cut(s) 461
Psp124BI GAGCTC 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 461
Psp6I CCWGG 2 cut(s) 457, 860
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 457, 860
PspN4I GGNNCC 5 cut(s) 15, 395, 431, 704, 829
PspPI GGNCC 5 cut(s) 233, 325, 461, 595, 762
PspPPI RGGWCCY 1 cut(s) 461
PspXI VCTCGAGB 1 cut(s) 964
PsrI GAACNNNNNNTAC 2 cut(s) 207, 239
PstNI CAGNNNCTG 1 cut(s) 464
RsaI GTAC 2 cut(s) 801, 940
RsaNI GTAC 2 cut(s) 800, 939
RseI CAYNNNNRTG 1 cut(s) 741
SacI GAGCTC 1 cut(s) 155
SacII CCGCGG 1 cut(s) 507
SaqAI TTAA 3 cut(s) 477, 498, 911
SatI GCNGC 1 cut(s) 747
Sau96I GGNCC 5 cut(s) 233, 325, 461, 595, 762
ScrFI CCNGG 2 cut(s) 459, 862
SduI GDGCHC 2 cut(s) 155, 398
SfaNI GCATC 3 cut(s) 640, 718, 722
SfcI CTRYAG 1 cut(s) 901
SfoI GGCGCC 1 cut(s) 431
Sfr274I CTCGAG 1 cut(s) 964
Sfr303I CCGCGG 1 cut(s) 507
SgrBI CCGCGG 1 cut(s) 507
SinI GGWCC 2 cut(s) 325, 461
SlaI CTCGAG 1 cut(s) 964
SmiMI CAYNNNNRTG 1 cut(s) 741
SmlI CTYRAG 2 cut(s) 154, 964
SmoI CTYRAG 2 cut(s) 154, 964
Sse9I AATT 4 cut(s) 70, 436, 560, 789
SseBI AGGCCT 1 cut(s) 874
SsiI CCGC 6 cut(s) 97, 504, 506, 513, 813, 841
SspDI GGCGCC 1 cut(s) 429
SspMI CTAG 1 cut(s) 375
SstI GAGCTC 1 cut(s) 155
StuI AGGCCT 1 cut(s) 874
StyD4I CCNGG 2 cut(s) 457, 860
TaaI ACNGT 2 cut(s) 334, 627
TaqI TCGA 3 cut(s) 698, 726, 965
TasI AATT 4 cut(s) 70, 436, 560, 789
TatI WGTACW 2 cut(s) 799, 938
TfiI GAWTC 1 cut(s) 670
Tru1I TTAA 3 cut(s) 477, 498, 911
Tru9I TTAA 3 cut(s) 477, 498, 911
TscAI CASTG 2 cut(s) 632, 756
TseI GCWGC 1 cut(s) 746
TspDTI ATGAA 3 cut(s) 228, 483, 976
TspGWI ACGGA 1 cut(s) 929
TspRI CASTG 2 cut(s) 632, 756
Van91I CCANNNNNTGG 1 cut(s) 861
VpaK11BI GGWCC 2 cut(s) 325, 461
XapI RAATTY 2 cut(s) 436, 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 858
XhoI CTCGAG 1 cut(s) 964
XmiI GTMKAC 1 cut(s) 845
XspI CTAG 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.