MD15G1161300.v1.1

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002821
Physical Location & Seq
Forward (+)
12123243 .. 12126059
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1161300.v1.1.491

Sequence Viewer

Length: 975 bp
ATGGCTTCTTCGTCTCTTCACCAAGCAACGGAAACCGGAAATGCAAGCTCCCTACTTACTTTCCAACCCGAAACTCAAAGCTCCGGCGATGGCATTGAGCTGTTAGCATGCCCTGTGTGCTACGAACCGCTCATCCGAAAAGGCCCCCCCGGTCTCAACCTGCAAGCAATATTTAGGTCGGCTTTCGAGTGCAAGATATGCCACAAGTTGTATAACAGCAGTAGTAAGAGCAAAGAGAAAGACATTTACCTGGATCTTACTGTTAGCGGTGGATTGAGGGAGTACATTGCGCCCATTCCAGCCGGGACTGACTTCTTCAGATATCGCCTCGTTTCCTTCTTGTACGAAAGAGGTTGGCGTCAGTATTTTGGCCAAAGCGGCTTCCCTGGACCTGATCAAGAGTTTAAAATGGCTATGGACTACTTTCAATCCGCTACTGATCAAGGTGAAGGTGGCGGTAGTGGTGGTGTAGTTGTGGATGTAAGCTGTGGGAGCGGTTTATTTTCCAGAAGATTTGCCAAATCCGGGGTCTTTGCTGGAGTAGTGGCGCTCGATTTTTCTGAGAGTATGCTTCGCCAGTGTTACAATTTTCTGAAGGCAGATCCTGCTTCCCTTTTTGCTACCAATCTTGGTCTCGTGAGGGCAGATGTTTCTAGGCTTCCATTTGCATCAGGTTCTGTTGATGCTGTCCACGCCGGCGCTGCATTGCATTGCTGGCCGTCTCCATCCAACGCCGTTGCTGAAATCACTCGTATATTACGCAGTGGCGCCATCTTTGTGGGAACGACTTTTCTGCGTTCTAGTCCTTATACTCCATGGATATTCAAGCCTTTTAGGGAGATGTCACGGAGCTCCAATAGCTATTTAACGGAGAGAGAGATTGAGGACTTGTGTAGATCATGCGGTCTCACCAACTACTCGAGCAAAGTTCAGCAATCCTTCATCATGTTTTCTGCTCGGAAACCGGCTGTGTAG

Protein Analysis

325

Amino Acids

35.43

Weight (kDa)

8.32

Isoelectric Point (pI)

58.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 148 - 264 1.9e-08 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 154 - 265 2.7e-11 Methyltransferase domain
Methyltransf_23 PF13489 154 - 307 7.3e-08 Methyltransferase domain
Methyltransf_25 PF13649 158 - 256 1.2e-15 Methyltransferase domain
Methyltransf_11 PF08241 159 - 260 7.6e-19 Methyltransferase domain
Methyltransf_12 PF08242 159 - 259 2.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 168
AccB1I GGYRCC 1 cut(s) 767
AccBSI CCGCTC 2 cut(s) 130, 495
AciI CCGC 7 cut(s) 128, 267, 378, 432, 456, 495, 903
AclWI GGATC 2 cut(s) 261, 596
AcoI YGGCCR 2 cut(s) 370, 716
AcuI CTGAAG 2 cut(s) 301, 614
AcyI GRCGYC 2 cut(s) 358, 768
AfaI GTAC 2 cut(s) 284, 344
AfiI CCNNNNNNNGG 2 cut(s) 28, 525
AgsI TTSAA 2 cut(s) 428, 826
AjnI CCWGG 2 cut(s) 249, 385
AloI GAACNNNNNNTCC 2 cut(s) 117, 149
AluBI AGCT 6 cut(s) 48, 81, 100, 486, 852, 861
AluI AGCT 6 cut(s) 48, 81, 100, 486, 852, 861
Alw21I GWGCWC 1 cut(s) 854
Alw26I GTCTC 5 cut(s) 18, 158, 638, 726, 911
AlwI GGATC 2 cut(s) 261, 596
AlwNI CAGNNNCTG 2 cut(s) 605, 677
Ama87I CYCGRG 1 cut(s) 919
AoxI GGCC 3 cut(s) 142, 370, 716
ApeKI GCWGC 1 cut(s) 701
ArsI GACNNNNNNTTYG 2 cut(s) 513, 545
AspLEI GCGC 4 cut(s) 292, 550, 701, 770
AspS9I GGNCC 2 cut(s) 143, 389
AsuC2I CCSGG 3 cut(s) 150, 304, 526
AsuHPI GGTGA 3 cut(s) 11, 458, 901
AvaI CYCGRG 1 cut(s) 919
AvaII GGWCC 1 cut(s) 389
BalI TGGCCA 1 cut(s) 372
BanI GGYRCC 1 cut(s) 767
BanII GRGCYC 1 cut(s) 854
BauI CACGAG 1 cut(s) 635
Bbv12I GWGCWC 1 cut(s) 854
BbvI GCAGC 1 cut(s) 688
BccI CCATC 3 cut(s) 83, 733, 779
BceAI ACGGC 2 cut(s) 703, 719
BcgI CGANNNNNNTGC 2 cut(s) 775, 809
BciT130I CCWGG 2 cut(s) 251, 387
BclI TGATCA 2 cut(s) 394, 439
BcnI CCSGG 3 cut(s) 150, 304, 526
BcoDI GTCTC 5 cut(s) 18, 158, 638, 726, 911
BfaI CTAG 2 cut(s) 654, 801
BfoI RGCGCY 3 cut(s) 551, 702, 771
BfuAI ACCTGC 1 cut(s) 168
BglI GCCNNNNNGGC 1 cut(s) 378
BisI GCNGC 2 cut(s) 379, 702
BlsI GCNGC 2 cut(s) 380, 703
Bme1390I CCNGG 5 cut(s) 150, 251, 304, 387, 526
Bme18I GGWCC 1 cut(s) 389
BmeT110I CYCGRG 1 cut(s) 919
BmgT120I GGNCC 2 cut(s) 143, 389
BmiI GGNNCC 2 cut(s) 145, 769
BmrFI CCNGG 5 cut(s) 150, 251, 304, 387, 526
BmsI GCATC 2 cut(s) 673, 677
BpmI CTGGAG 1 cut(s) 558
BpuMI CCSGG 3 cut(s) 150, 304, 526
BsaHI GRCGYC 2 cut(s) 358, 768
BsaI GGTCTC 3 cut(s) 158, 638, 911
BsaJI CCNNGG 4 cut(s) 148, 385, 525, 815
BsaWI WCCGGW 1 cut(s) 35
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 525
Bse118I RCCGGY 2 cut(s) 695, 964
Bse1I ACTGG 1 cut(s) 577
Bse3DI GCAATG 3 cut(s) 285, 704, 709
BseBI CCWGG 2 cut(s) 251, 387
BseDI CCNNGG 4 cut(s) 148, 385, 525, 815
BseGI GGATG 3 cut(s) 132, 484, 725
BseLI CCNNNNNNNGG 2 cut(s) 28, 525
BseMI GCAATG 3 cut(s) 285, 704, 709
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 1 cut(s) 577
BseXI GCAGC 1 cut(s) 688
BshFI GGCC 3 cut(s) 144, 372, 718
BshNI GGYRCC 1 cut(s) 767
BsiHKAI GWGCWC 1 cut(s) 854
BsiHKCI CYCGRG 1 cut(s) 919
BsiSI CCGG 7 cut(s) 36, 84, 150, 303, 525, 696, 965
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 2 cut(s) 28, 525
BsmAI GTCTC 5 cut(s) 18, 158, 638, 726, 911
BsmBI CGTCTC 2 cut(s) 18, 726
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 3 cut(s) 144, 372, 718
Bso31I GGTCTC 3 cut(s) 158, 638, 911
BsoBI CYCGRG 1 cut(s) 919
Bsp1286I GDGCHC 1 cut(s) 854
Bsp143I GATC 5 cut(s) 253, 394, 439, 601, 896
Bsp19I CCATGG 1 cut(s) 815
BspACI CCGC 7 cut(s) 128, 267, 378, 432, 456, 495, 903
BspANI GGCC 3 cut(s) 144, 372, 718
BspCNI CTCAG 1 cut(s) 553
BspLI GGNNCC 2 cut(s) 145, 769
BspMI ACCTGC 1 cut(s) 168
BspPI GGATC 2 cut(s) 261, 596
BspT107I GGYRCC 1 cut(s) 767
BspTNI GGTCTC 3 cut(s) 158, 638, 911
BsrBI CCGCTC 2 cut(s) 130, 495
BsrDI GCAATG 3 cut(s) 285, 704, 709
BsrFI RCCGGY 2 cut(s) 695, 964
BsrI ACTGG 1 cut(s) 577
BssAI RCCGGY 2 cut(s) 695, 964
BssECI CCNNGG 4 cut(s) 148, 385, 525, 815
BssMI GATC 5 cut(s) 253, 394, 439, 601, 896
BssNI GRCGYC 2 cut(s) 358, 768
BssSI CACGAG 1 cut(s) 635
BssT1I CCWWGG 1 cut(s) 815
Bst2BI CACGAG 1 cut(s) 635
Bst2UI CCWGG 2 cut(s) 251, 387
Bst4CI ACNGT 1 cut(s) 262
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 2 cut(s) 358, 768
BstAPI GCANNNNNTGC 2 cut(s) 198, 605
BstC8I GCNNGC 5 cut(s) 46, 109, 165, 697, 716
BstDEI CTNAG 1 cut(s) 561
BstDSI CCRYGG 1 cut(s) 815
BstF5I GGATG 3 cut(s) 132, 484, 725
BstH2I RGCGCY 3 cut(s) 551, 702, 771
BstHHI GCGC 4 cut(s) 292, 550, 701, 770
BstKTI GATC 5 cut(s) 256, 397, 442, 604, 899
BstMAI GTCTC 5 cut(s) 18, 158, 638, 726, 911
BstMBI GATC 5 cut(s) 253, 394, 439, 601, 896
BstMWI GCNNNNNNNGC 9 cut(s) 117, 198, 378, 492, 605, 692, 701, 715, 858
BstNI CCWGG 2 cut(s) 251, 387
BstNSI RCATGY 1 cut(s) 111
BstSCI CCNGG 5 cut(s) 148, 249, 302, 385, 524
BstV1I GCAGC 1 cut(s) 688
BstX2I RGATCY 2 cut(s) 253, 601
BstXI CCANNNNNNTGG 1 cut(s) 778
BstYI RGATCY 2 cut(s) 253, 601
BsuRI GGCC 3 cut(s) 144, 372, 718
BtgI CCRYGG 1 cut(s) 815
BtgZI GCGATG 1 cut(s) 102
BtsCI GGATG 3 cut(s) 132, 484, 725
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 2 cut(s) 584, 769
BveI ACCTGC 1 cut(s) 168
Cac8I GCNNGC 5 cut(s) 46, 109, 165, 697, 716
CaiI CAGNNNCTG 2 cut(s) 605, 677
CfoI GCGC 4 cut(s) 292, 550, 701, 770
Cfr10I RCCGGY 2 cut(s) 695, 964
Cfr13I GGNCC 2 cut(s) 143, 389
CseI GACGC 1 cut(s) 347
Csp6I GTAC 2 cut(s) 283, 343
CviAII CATG 4 cut(s) 108, 816, 900, 946
CviQI GTAC 2 cut(s) 283, 343
DdeI CTNAG 1 cut(s) 561
DinI GGCGCC 1 cut(s) 769
DpnI GATC 5 cut(s) 255, 396, 441, 603, 898
DpnII GATC 5 cut(s) 253, 394, 439, 601, 896
DraI TTTAAA 1 cut(s) 406
EaeI YGGCCR 2 cut(s) 370, 716
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 852
Eco130I CCWWGG 1 cut(s) 815
Eco24I GRGCYC 1 cut(s) 854
Eco31I GGTCTC 3 cut(s) 158, 638, 911
Eco32I GATATC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 389
Eco53kI GAGCTC 1 cut(s) 852
Eco57I CTGAAG 2 cut(s) 301, 614
Eco88I CYCGRG 1 cut(s) 919
EcoICRI GAGCTC 1 cut(s) 852
EcoO109I RGGNCCY 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 249, 385
EcoRV GATATC 1 cut(s) 323
EcoT14I CCWWGG 1 cut(s) 815
EcoT38I GRGCYC 1 cut(s) 854
EgeI GGCGCC 1 cut(s) 769
EheI GGCGCC 1 cut(s) 769
ErhI CCWWGG 1 cut(s) 815
Esp3I CGTCTC 2 cut(s) 18, 726
FaeI CATG 4 cut(s) 111, 819, 903, 949
FaqI GGGAC 1 cut(s) 319
FatI CATG 4 cut(s) 107, 815, 899, 945
FbaI TGATCA 2 cut(s) 394, 439
Fnu4HI GCNGC 2 cut(s) 379, 702
FokI GGATG 3 cut(s) 119, 491, 712
FriOI GRGCYC 1 cut(s) 854
Fsp4HI GCNGC 2 cut(s) 379, 702
FspBI CTAG 2 cut(s) 654, 801
GlaI GCGC 4 cut(s) 291, 549, 700, 769
GluI GCNGC 2 cut(s) 379, 702
GsuI CTGGAG 1 cut(s) 558
HaeII RGCGCY 3 cut(s) 551, 702, 771
HaeIII GGCC 3 cut(s) 144, 372, 718
HapII CCGG 7 cut(s) 36, 84, 150, 303, 525, 696, 965
HgaI GACGC 1 cut(s) 347
HhaI GCGC 4 cut(s) 292, 550, 701, 770
Hin1I GRCGYC 2 cut(s) 358, 768
Hin1II CATG 4 cut(s) 111, 819, 903, 949
Hin6I GCGC 4 cut(s) 290, 548, 699, 768
HinP1I GCGC 4 cut(s) 290, 548, 699, 768
HpaII CCGG 7 cut(s) 36, 84, 150, 303, 525, 696, 965
HphI GGTGA 3 cut(s) 11, 458, 901
Hpy166II GTNNAC 1 cut(s) 691
Hpy188I TCNGA 5 cut(s) 137, 320, 562, 594, 960
Hpy188III TCNNGA 3 cut(s) 398, 507, 637
Hpy8I GTNNAC 1 cut(s) 691
HpyAV CCTTC 4 cut(s) 346, 443, 589, 949
HpyCH4III ACNGT 1 cut(s) 262
HpyCH4V TGCA 6 cut(s) 44, 163, 192, 668, 704, 709
HpyF10VI GCNNNNNNNGC 9 cut(s) 117, 198, 378, 492, 605, 692, 701, 715, 858
HpyF3I CTNAG 1 cut(s) 561
Hsp92I GRCGYC 2 cut(s) 358, 768
Hsp92II CATG 4 cut(s) 111, 819, 903, 949
HspAI GCGC 4 cut(s) 290, 548, 699, 768
KasI GGCGCC 1 cut(s) 767
KroI GCCGGC 1 cut(s) 695
KroNI GCCGGC 1 cut(s) 697
Ksp22I TGATCA 2 cut(s) 394, 439
Kzo9I GATC 5 cut(s) 253, 394, 439, 601, 896
LmnI GCTCC 5 cut(s) 53, 86, 492, 849, 857
Lsp1109I GCAGC 1 cut(s) 688
LweI GCATC 2 cut(s) 673, 677
MaeI CTAG 2 cut(s) 654, 801
MaeIII GTNAC 2 cut(s) 581, 843
MalI GATC 5 cut(s) 255, 396, 441, 603, 898
MbiI CCGCTC 2 cut(s) 130, 495
MboI GATC 5 cut(s) 253, 394, 439, 601, 896
MboII GAAGA 3 cut(s) 8, 307, 522
MflI RGATCY 2 cut(s) 253, 601
MhlI GDGCHC 1 cut(s) 854
MlsI TGGCCA 1 cut(s) 372
MluCI AATT 1 cut(s) 586
MluNI TGGCCA 1 cut(s) 372
Mly113I GGCGCC 1 cut(s) 768
MmeI TCCRAC 2 cut(s) 88, 753
MnlI CCTC 5 cut(s) 270, 338, 344, 633, 877
Mox20I TGGCCA 1 cut(s) 372
MreI CGCCGGCG 1 cut(s) 695
MroNI GCCGGC 1 cut(s) 695
MscI TGGCCA 1 cut(s) 372
MseI TTAA 2 cut(s) 405, 866
MslI CAYNNNNRTG 1 cut(s) 776
Msp20I TGGCCA 1 cut(s) 372
MspI CCGG 7 cut(s) 36, 84, 150, 303, 525, 696, 965
MspR9I CCNGG 5 cut(s) 150, 251, 304, 387, 526
MvaI CCWGG 2 cut(s) 251, 387
MwoI GCNNNNNNNGC 9 cut(s) 117, 198, 378, 492, 605, 692, 701, 715, 858
NaeI GCCGGC 1 cut(s) 697
NarI GGCGCC 1 cut(s) 768
NciI CCSGG 3 cut(s) 150, 304, 526
NcoI CCATGG 1 cut(s) 815
NdeII GATC 5 cut(s) 253, 394, 439, 601, 896
NgoMIV GCCGGC 1 cut(s) 695
NlaIII CATG 4 cut(s) 111, 819, 903, 949
NlaIV GGNNCC 2 cut(s) 145, 769
NmuCI GTSAC 1 cut(s) 843
NspI RCATGY 1 cut(s) 111
PaeI GCATGC 1 cut(s) 111
PaeR7I CTCGAG 1 cut(s) 919
PdiI GCCGGC 1 cut(s) 697
PkrI GCNGC 2 cut(s) 380, 703
PluTI GGCGCC 1 cut(s) 771
Psp124BI GAGCTC 1 cut(s) 854
Psp6I CCWGG 2 cut(s) 249, 385
PspGI CCWGG 2 cut(s) 249, 385
PspN4I GGNNCC 2 cut(s) 145, 769
PspPI GGNCC 2 cut(s) 143, 389
PspXI VCTCGAGB 1 cut(s) 919
PstNI CAGNNNCTG 2 cut(s) 605, 677
PsuI RGATCY 2 cut(s) 253, 601
RsaI GTAC 2 cut(s) 284, 344
RsaNI GTAC 2 cut(s) 283, 343
RseI CAYNNNNRTG 1 cut(s) 776
SacI GAGCTC 1 cut(s) 854
SaqAI TTAA 2 cut(s) 405, 866
SatI GCNGC 2 cut(s) 379, 702
Sau3AI GATC 5 cut(s) 253, 394, 439, 601, 896
Sau96I GGNCC 2 cut(s) 143, 389
ScrFI CCNGG 5 cut(s) 150, 251, 304, 387, 526
SduI GDGCHC 1 cut(s) 854
SfaNI GCATC 2 cut(s) 673, 677
SfoI GGCGCC 1 cut(s) 769
Sfr274I CTCGAG 1 cut(s) 919
SgrAI CRCCGGYG 1 cut(s) 695
SinI GGWCC 1 cut(s) 389
SlaI CTCGAG 1 cut(s) 919
SmiMI CAYNNNNRTG 1 cut(s) 776
SmlI CTYRAG 1 cut(s) 919
SmoI CTYRAG 1 cut(s) 919
SphI GCATGC 1 cut(s) 111
Sse9I AATT 1 cut(s) 586
SsiI CCGC 7 cut(s) 128, 267, 378, 432, 456, 495, 903
SspDI GGCGCC 1 cut(s) 767
SspI AATATT 1 cut(s) 171
SspMI CTAG 2 cut(s) 654, 801
SstI GAGCTC 1 cut(s) 854
StyD4I CCNGG 5 cut(s) 148, 249, 302, 385, 524
StyI CCWWGG 1 cut(s) 815
TaaI ACNGT 1 cut(s) 262
TaqI TCGA 3 cut(s) 186, 552, 920
TasI AATT 1 cut(s) 586
TatI WGTACW 1 cut(s) 282
TauI GCSGC 1 cut(s) 381
Tru1I TTAA 2 cut(s) 405, 866
Tru9I TTAA 2 cut(s) 405, 866
TscAI CASTG 2 cut(s) 584, 769
TseFI GTSAC 1 cut(s) 843
TseI GCWGC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 843
TspDTI ATGAA 1 cut(s) 931
TspGWI ACGGA 3 cut(s) 44, 862, 884
TspRI CASTG 2 cut(s) 584, 769
VpaK11BI GGWCC 1 cut(s) 389
XceI RCATGY 1 cut(s) 111
XhoI CTCGAG 1 cut(s) 919
XspI CTAG 2 cut(s) 654, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.