RLG00000015814

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002821
Physical Location & Seq
Forward (+)
1603749 .. 1606299
2551 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015814

Sequence Viewer

Length: 1104 bp
ATGGCGACGACGGCTCCCTCCTCCCTCCACCAAACATTTCTATCCCCAAAACACCCTCGCCTCCACCACAATTCCCATCTCTCTCCTTCTCGTCTCCGCTTCCACTCTCTCCCCATTCGCGCCACCTCTGCGGTCACTCTGGAGAAGAGCTCGAGCACCCAGAAAAGTCAAAGCTCGGGCATTGAGATTTTGTCATGCCCGGTTTGCTATGAACCGCTAATACGAAAAGGCCCAACTGGTCTAAACTTGGAAGCAATATACAGGTCAGGTTTCAAGTGCAAGAAATGTGACAAGTCGTATTCCAGCAAAGACACTTACTTGGACCTTACTGTTACTGCTGGGTTGAAGCAGTACGTTGAAGTCAACCCAGCTAGGACTGAGTTGTTTAGGAGCCCACTCGTGTCGTTTTTGTATGAAAGAGGTTGGCGCCAGAATTTTAGTAGTAGTGGATTTCCAGGTCCTGATGAAGAGTTTAAAATGGCTCAGGACTACTTTAAATCCGCGGAAGGCGGTTTTCTAGTGGATGTGAGCTGTGGGAGTGGTTTGTTTTCAAGGAAATTTGCGAAATCGGGGAGTTATTCTGGAGTTGTGGCGCTTGATTTTTCGGAAAATATGCTCCGACAGTGCTATGAATTTCTTAAAAGAGATGCTACTCTTTTGGCTACGAATCTTGCTCTTGTAAGGGCAGATGTTTCTAGGCTTCCATTTGCAACAGGTTCAGTCGATGCAGTCCATGCCGGTGCAGCACTGCACTGTTGGCCCTCTCCTTCAAATGCTTTTTCAATATTTGCTCAATTTAAACATGTGTATGGATGGAATAAAACTTCAGTAACTAATATATTTACCATCTCTTTTCTGATATATTTCCAGGTTGCTGAAATCACTCGTGTATTACGAAGTGGTGGTGTCTTTGTTGGAACCACTTTTCTGCGGTATACTTCATCCACTCCCTGGTATATCAGGCCTTTCAGAGAGAGGACTGCTCAGAGCTACAGCTACCTAACGGAGGAAGAGATACGGGACTTGTGTACATCATGTGGTCTCACGAACTACTCGAGCAAAGTTCAGCAGTCTTTCATCATGTTTTCTGCTCAGAAACCTTGA

Protein Analysis

368

Amino Acids

41.05

Weight (kDa)

9.31

Isoelectric Point (pI)

48.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 166 - 247 1e-06 ubiE/COQ5 methyltransferase family
Methyltransf_11 PF08241 174 - 261 8.6e-14 Methyltransferase domain
Methyltransf_25 PF13649 174 - 257 3.4e-13 Methyltransferase domain
Methyltransf_31 PF13847 174 - 309 3.4e-10 Methyltransferase domain
Methyltransf_12 PF08242 174 - 261 7.1e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 426
AccB7I CCANNNNNTGG 1 cut(s) 951
AccI GTMKAC 1 cut(s) 935
AccII CGCG 2 cut(s) 120, 503
AciI CCGC 7 cut(s) 97, 131, 215, 501, 503, 510, 931
AcsI RAATTY 3 cut(s) 433, 557, 632
AcuI CTGAAG 1 cut(s) 810
AcyI GRCGYC 1 cut(s) 427
AfaI GTAC 2 cut(s) 353, 1030
AfiI CCNNNNNNNGG 2 cut(s) 951, 1006
AflIII ACRYGT 1 cut(s) 802
AgsI TTSAA 6 cut(s) 274, 346, 359, 552, 771, 783
AjnI CCWGG 3 cut(s) 454, 867, 950
AluBI AGCT 6 cut(s) 150, 174, 371, 531, 990, 996
AluI AGCT 6 cut(s) 150, 174, 371, 531, 990, 996
Alw21I GWGCWC 2 cut(s) 152, 158
Alw26I GTCTC 2 cut(s) 98, 1046
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 3 cut(s) 151, 175, 1054
AoxI GGCC 3 cut(s) 229, 758, 962
ApeKI GCWGC 1 cut(s) 743
ApoI RAATTY 3 cut(s) 433, 557, 632
AspLEI GCGC 3 cut(s) 122, 429, 595
AspS9I GGNCC 4 cut(s) 230, 322, 458, 759
AsuC2I CCSGG 1 cut(s) 200
AvaI CYCGRG 3 cut(s) 151, 175, 1054
AvaII GGWCC 2 cut(s) 322, 458
BanI GGYRCC 1 cut(s) 426
BanII GRGCYC 2 cut(s) 152, 395
BauI CACGAG 2 cut(s) 398, 885
Bbv12I GWGCWC 2 cut(s) 152, 158
BbvI GCAGC 1 cut(s) 755
BccI CCATC 3 cut(s) 84, 807, 854
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 3 cut(s) 456, 869, 952
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 2 cut(s) 98, 1046
BfaI CTAG 3 cut(s) 372, 518, 696
BfmI CTRYAG 1 cut(s) 991
BfoI RGCGCY 2 cut(s) 430, 596
BisI GCNGC 1 cut(s) 744
BlsI GCNGC 1 cut(s) 745
Bme1390I CCNGG 4 cut(s) 200, 456, 869, 952
Bme18I GGWCC 2 cut(s) 322, 458
BmeT110I CYCGRG 3 cut(s) 151, 175, 1054
BmgT120I GGNCC 4 cut(s) 230, 322, 458, 759
BmiI GGNNCC 4 cut(s) 15, 392, 428, 919
BmrFI CCNGG 4 cut(s) 200, 456, 869, 952
BmsI GCATC 2 cut(s) 637, 715
BplI GAGNNNNNCTC 4 cut(s) 134, 166, 967, 999
BpmI CTGGAG 2 cut(s) 161, 603
Bpu10I CCTNAGC 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 427
BsaI GGTCTC 1 cut(s) 1046
BsaJI CCNNGG 2 cut(s) 501, 950
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 2 cut(s) 951, 1006
Bse118I RCCGGY 1 cut(s) 737
Bse1I ACTGG 1 cut(s) 241
BseBI CCWGG 3 cut(s) 456, 869, 952
BseDI CCNNGG 2 cut(s) 501, 950
BseGI GGATG 3 cut(s) 529, 818, 941
BseLI CCNNNNNNNGG 2 cut(s) 951, 1006
BseMII CTCAG 3 cut(s) 369, 497, 998
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 1 cut(s) 10
BseXI GCAGC 1 cut(s) 755
BseYI CCCAGC 2 cut(s) 338, 367
BsgI GTGCAG 2 cut(s) 734, 762
Bsh1236I CGCG 2 cut(s) 120, 503
BshFI GGCC 3 cut(s) 231, 760, 964
BshNI GGYRCC 1 cut(s) 426
BsiHKAI GWGCWC 2 cut(s) 152, 158
BsiHKCI CYCGRG 3 cut(s) 151, 175, 1054
BsiSI CCGG 2 cut(s) 200, 738
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 2 cut(s) 951, 1006
BsmAI GTCTC 2 cut(s) 98, 1046
BsmBI CGTCTC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 1034
BsnI GGCC 3 cut(s) 231, 760, 964
Bso31I GGTCTC 1 cut(s) 1046
BsoBI CYCGRG 3 cut(s) 151, 175, 1054
Bsp1286I GDGCHC 3 cut(s) 152, 158, 395
Bsp1407I TGTACA 1 cut(s) 1028
BspACI CCGC 7 cut(s) 97, 131, 215, 501, 503, 510, 931
BspANI GGCC 3 cut(s) 231, 760, 964
BspCNI CTCAG 3 cut(s) 370, 496, 997
BspFNI CGCG 2 cut(s) 120, 503
BspLI GGNNCC 4 cut(s) 15, 392, 428, 919
BspQI GCTCTTC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 426
BspTNI GGTCTC 1 cut(s) 1046
BsrFI RCCGGY 1 cut(s) 737
BsrGI TGTACA 1 cut(s) 1028
BsrI ACTGG 1 cut(s) 241
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 2 cut(s) 501, 950
BssNAI GTATAC 1 cut(s) 936
BssNI GRCGYC 1 cut(s) 427
BssSI CACGAG 2 cut(s) 398, 885
Bst1107I GTATAC 1 cut(s) 936
Bst2BI CACGAG 2 cut(s) 398, 885
Bst2UI CCWGG 3 cut(s) 456, 869, 952
Bst4CI ACNGT 3 cut(s) 331, 624, 755
Bst6I CTCTTC 3 cut(s) 140, 462, 1005
BstACI GRCGYC 1 cut(s) 427
BstAPI GCANNNNNTGC 1 cut(s) 734
BstAUI TGTACA 1 cut(s) 1028
BstDEI CTNAG 4 cut(s) 378, 483, 984, 1092
BstDSI CCRYGG 1 cut(s) 501
BstENI CCTNNNNNAGG 1 cut(s) 1004
BstF5I GGATG 3 cut(s) 529, 818, 941
BstFNI CGCG 2 cut(s) 120, 503
BstH2I RGCGCY 2 cut(s) 430, 596
BstHHI GCGC 3 cut(s) 122, 429, 595
BstMAI GTCTC 2 cut(s) 98, 1046
BstMWI GCNNNNNNNGC 6 cut(s) 11, 128, 204, 734, 743, 757
BstNI CCWGG 3 cut(s) 456, 869, 952
BstNSI RCATGY 1 cut(s) 806
BstSCI CCNGG 4 cut(s) 198, 454, 867, 950
BstSFI CTRYAG 1 cut(s) 991
BstUI CGCG 2 cut(s) 120, 503
BstV1I GCAGC 1 cut(s) 755
BstZ17I GTATAC 1 cut(s) 936
BsuRI GGCC 3 cut(s) 231, 760, 964
BtgI CCRYGG 1 cut(s) 501
BtsCI GGATG 3 cut(s) 529, 818, 941
BtsI GCAGTG 1 cut(s) 746
BtsIMutI CAGTG 3 cut(s) 629, 746, 751
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 3 cut(s) 122, 429, 595
Cfr10I RCCGGY 1 cut(s) 737
Cfr13I GGNCC 4 cut(s) 230, 322, 458, 759
Cfr42I CCGCGG 1 cut(s) 504
Csp6I GTAC 2 cut(s) 352, 1029
CviAII CATG 5 cut(s) 195, 734, 803, 1035, 1081
CviQI GTAC 2 cut(s) 352, 1029
DdeI CTNAG 4 cut(s) 378, 483, 984, 1092
DinI GGCGCC 1 cut(s) 428
DraI TTTAAA 3 cut(s) 475, 496, 799
Eam1104I CTCTTC 3 cut(s) 140, 462, 1005
EarI CTCTTC 3 cut(s) 140, 462, 1005
Ecl136II GAGCTC 1 cut(s) 150
Eco147I AGGCCT 1 cut(s) 964
Eco24I GRGCYC 2 cut(s) 152, 395
Eco31I GGTCTC 1 cut(s) 1046
Eco47I GGWCC 2 cut(s) 322, 458
Eco53kI GAGCTC 1 cut(s) 150
Eco57I CTGAAG 1 cut(s) 810
Eco88I CYCGRG 3 cut(s) 151, 175, 1054
EcoICRI GAGCTC 1 cut(s) 150
EcoNI CCTNNNNNAGG 1 cut(s) 1004
EcoO109I RGGNCCY 1 cut(s) 458
EcoRII CCWGG 3 cut(s) 454, 867, 950
EcoT38I GRGCYC 2 cut(s) 152, 395
EgeI GGCGCC 1 cut(s) 428
EheI GGCGCC 1 cut(s) 428
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 5 cut(s) 198, 737, 806, 1038, 1084
FaqI GGGAC 1 cut(s) 1034
FatI CATG 5 cut(s) 194, 733, 802, 1034, 1080
FblI GTMKAC 1 cut(s) 935
Fnu4HI GCNGC 1 cut(s) 744
FokI GGATG 3 cut(s) 536, 825, 928
FriOI GRGCYC 2 cut(s) 152, 395
Fsp4HI GCNGC 1 cut(s) 744
FspBI CTAG 3 cut(s) 372, 518, 696
GlaI GCGC 3 cut(s) 121, 428, 594
GluI GCNGC 1 cut(s) 744
GsaI CCCAGC 2 cut(s) 342, 371
GsuI CTGGAG 2 cut(s) 161, 603
HaeII RGCGCY 2 cut(s) 430, 596
HaeIII GGCC 3 cut(s) 231, 760, 964
HapII CCGG 2 cut(s) 200, 738
HhaI GCGC 3 cut(s) 122, 429, 595
Hin1I GRCGYC 1 cut(s) 427
Hin1II CATG 5 cut(s) 198, 737, 806, 1038, 1084
Hin6I GCGC 3 cut(s) 120, 427, 593
HinP1I GCGC 3 cut(s) 120, 427, 593
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 1 cut(s) 667
HpaII CCGG 2 cut(s) 200, 738
Hpy166II GTNNAC 3 cut(s) 364, 936, 1029
Hpy188I TCNGA 6 cut(s) 607, 620, 858, 971, 987, 1095
Hpy188III TCNNGA 5 cut(s) 140, 461, 485, 582, 1045
Hpy8I GTNNAC 3 cut(s) 364, 936, 1029
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 3 cut(s) 96, 500, 777
HpyCH4III ACNGT 3 cut(s) 331, 624, 755
HpyCH4IV ACGT 1 cut(s) 354
HpyCH4V TGCA 5 cut(s) 279, 710, 728, 743, 751
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 128, 204, 734, 743, 757
HpyF3I CTNAG 4 cut(s) 378, 483, 984, 1092
HpySE526I ACGT 1 cut(s) 354
Hsp92I GRCGYC 1 cut(s) 427
Hsp92II CATG 5 cut(s) 198, 737, 806, 1038, 1084
HspAI GCGC 3 cut(s) 120, 427, 593
KasI GGCGCC 1 cut(s) 426
KspI CCGCGG 1 cut(s) 504
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 3 cut(s) 19, 390, 621
Lsp1109I GCAGC 1 cut(s) 755
LweI GCATC 2 cut(s) 637, 715
MaeI CTAG 3 cut(s) 372, 518, 696
MaeII ACGT 1 cut(s) 354
MaeIII GTNAC 4 cut(s) 133, 287, 331, 829
MboII GAAGA 3 cut(s) 157, 479, 1022
MhlI GDGCHC 3 cut(s) 152, 158, 395
MluCI AATT 5 cut(s) 70, 433, 557, 632, 794
Mly113I GGCGCC 1 cut(s) 427
MmeI TCCRAC 2 cut(s) 643, 895
MseI TTAA 4 cut(s) 474, 495, 639, 798
MslI CAYNNNNRTG 2 cut(s) 738, 807
MspA1I CMGCKG 1 cut(s) 503
MspI CCGG 2 cut(s) 200, 738
MspR9I CCNGG 4 cut(s) 200, 456, 869, 952
MvaI CCWGG 3 cut(s) 456, 869, 952
MvnI CGCG 2 cut(s) 120, 503
MwoI GCNNNNNNNGC 6 cut(s) 11, 128, 204, 734, 743, 757
NarI GGCGCC 1 cut(s) 427
NciI CCSGG 1 cut(s) 200
NlaIII CATG 5 cut(s) 198, 737, 806, 1038, 1084
NlaIV GGNNCC 4 cut(s) 15, 392, 428, 919
NmuCI GTSAC 2 cut(s) 133, 287
NspI RCATGY 1 cut(s) 806
PaeR7I CTCGAG 2 cut(s) 151, 1054
PceI AGGCCT 1 cut(s) 964
PciI ACATGT 1 cut(s) 802
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 2 cut(s) 892, 1052
PfeI GAWTC 1 cut(s) 667
PflMI CCANNNNNTGG 1 cut(s) 951
PkrI GCNGC 1 cut(s) 745
PluTI GGCGCC 1 cut(s) 430
PpuMI RGGWCCY 1 cut(s) 458
PscI ACATGT 1 cut(s) 802
Psp124BI GAGCTC 1 cut(s) 152
Psp5II RGGWCCY 1 cut(s) 458
Psp6I CCWGG 3 cut(s) 454, 867, 950
PspFI CCCAGC 2 cut(s) 338, 367
PspGI CCWGG 3 cut(s) 454, 867, 950
PspN4I GGNNCC 4 cut(s) 15, 392, 428, 919
PspPI GGNCC 4 cut(s) 230, 322, 458, 759
PspPPI RGGWCCY 1 cut(s) 458
PspXI VCTCGAGB 2 cut(s) 151, 1054
PsrI GAACNNNNNNTAC 2 cut(s) 204, 236
PstNI CAGNNNCTG 1 cut(s) 461
RsaI GTAC 2 cut(s) 353, 1030
RsaNI GTAC 2 cut(s) 352, 1029
RseI CAYNNNNRTG 2 cut(s) 738, 807
SacI GAGCTC 1 cut(s) 152
SacII CCGCGG 1 cut(s) 504
SapI GCTCTTC 1 cut(s) 140
SaqAI TTAA 4 cut(s) 474, 495, 639, 798
SatI GCNGC 1 cut(s) 744
Sau96I GGNCC 4 cut(s) 230, 322, 458, 759
ScrFI CCNGG 4 cut(s) 200, 456, 869, 952
SduI GDGCHC 3 cut(s) 152, 158, 395
SfaNI GCATC 2 cut(s) 637, 715
SfcI CTRYAG 1 cut(s) 991
SfoI GGCGCC 1 cut(s) 428
Sfr274I CTCGAG 2 cut(s) 151, 1054
Sfr303I CCGCGG 1 cut(s) 504
SgrBI CCGCGG 1 cut(s) 504
SinI GGWCC 2 cut(s) 322, 458
SlaI CTCGAG 2 cut(s) 151, 1054
SmiMI CAYNNNNRTG 2 cut(s) 738, 807
SmlI CTYRAG 2 cut(s) 151, 1054
SmoI CTYRAG 2 cut(s) 151, 1054
Sse9I AATT 5 cut(s) 70, 433, 557, 632, 794
SseBI AGGCCT 1 cut(s) 964
SsiI CCGC 7 cut(s) 97, 131, 215, 501, 503, 510, 931
SspDI GGCGCC 1 cut(s) 426
SspI AATATT 1 cut(s) 786
SspMI CTAG 3 cut(s) 372, 518, 696
SstI GAGCTC 1 cut(s) 152
StuI AGGCCT 1 cut(s) 964
StyD4I CCNGG 4 cut(s) 198, 454, 867, 950
TaaI ACNGT 3 cut(s) 331, 624, 755
TaiI ACGT 1 cut(s) 357
TaqI TCGA 3 cut(s) 152, 723, 1055
TasI AATT 5 cut(s) 70, 433, 557, 632, 794
TatI WGTACW 1 cut(s) 1028
TfiI GAWTC 1 cut(s) 667
Tru1I TTAA 4 cut(s) 474, 495, 639, 798
Tru9I TTAA 4 cut(s) 474, 495, 639, 798
TscAI CASTG 3 cut(s) 629, 753, 758
TseFI GTSAC 2 cut(s) 133, 287
TseI GCWGC 1 cut(s) 743
Tsp45I GTSAC 2 cut(s) 133, 287
TspDTI ATGAA 6 cut(s) 225, 429, 480, 645, 930, 1066
TspGWI ACGGA 1 cut(s) 1019
TspRI CASTG 3 cut(s) 629, 753, 758
Van91I CCANNNNNTGG 1 cut(s) 951
VpaK11BI GGWCC 2 cut(s) 322, 458
XagI CCTNNNNNAGG 1 cut(s) 1004
XapI RAATTY 3 cut(s) 433, 557, 632
XceI RCATGY 1 cut(s) 806
XhoI CTCGAG 2 cut(s) 151, 1054
XmiI GTMKAC 1 cut(s) 935
XspI CTAG 3 cut(s) 372, 518, 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.