Rh2AG023300

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002821
Physical Location & Seq
Forward (+)
1656183 .. 1658842
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG023300.1

Sequence Viewer

Length: 912 bp
ATGGCCACGACGGCTCCCTCCTCCCTCCACCAAACATTTCTATCCCCAAAACACCCTCGCCTCCACCACAATTCCCATCTCTCTCCTCCTCGTCTCCGCTTCCACTCTCTCCCCATTCGCGCCACCTCTGCAATCACTCTACAACCGGAGAAGAGCTGGAGCACCCAGAAAAGTCAAAGCTCGGGCATTGAGTTTTTGTCATGCCCAGTTTGCTATGAACCGCTAATACGAAAAGGCCCAACTGGTCTAAACCTGGAAGCAATATACAGGTCAGGTTTCAAGTGCAAGAAATGTGACAAGTCGTTTTCCAGCAAAGACATTTACTTGGACCTTACTGTTACTGCTGGGTTGAAGCAGTACGTTGAAGTCAACCCAGCTAGGACTGAGTTGTTTAGGAGCCCACTTGTGTCGTTTTTGTATGAAAGAGGTTGGCGCCAGAATTTTAGCACTAGTGGATTTCCGGGTCCTGATGAAGAGTTTAAAATGGCTCAGGACTACTTTAAATCCGCGGAAGGCGGTTTTCTAGTGGATGTGAGCTGTGGGAGTGGTTTGTTTTCGAGGAAATTTGCGAAATCGGGGAGTTATTCTGGAGTTGTGGCGCTTGATTTTTCGGAAAATATGCTCCGACAGTGCTATGATTTTCTTAAAAGAGATGCTACTCTTTTGGCTACGAATCTTGCTCTTGTAAGGGCAGATGTTTCTAGGCTTCCATTTGCATCAGGTTCAGTCGATGCAGTCCATGCCGGTGCAGCACTGCACTGTTGGCCCTCTCCTTCAAATGCTAGGACTGCTCAGAGCTACAGCTACCTAACGGAGGAAGAGATACGGGACTTGTGTACATCATGTGGTCTCACGAACTACTCGAGCAAAGTTCAGCAATCTTTCATCATGTTTTCTGCTCAGAAACCTTGA

Protein Analysis

303

Amino Acids

33.63

Weight (kDa)

9.0

Isoelectric Point (pI)

50.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 167 - 249 1.1e-06 ubiE/COQ5 methyltransferase family
Methyltransf_11 PF08241 176 - 261 2.8e-13 Methyltransferase domain
Methyltransf_25 PF13649 176 - 263 3.2e-13 Methyltransferase domain
Methyltransf_12 PF08242 176 - 259 3.4e-07 Methyltransferase domain
Methyltransf_31 PF13847 176 - 260 6.6e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 432
AccII CGCG 2 cut(s) 120, 509
AciI CCGC 5 cut(s) 97, 221, 507, 509, 516
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 439, 563
AcyI GRCGYC 1 cut(s) 433
AfaI GTAC 2 cut(s) 359, 838
AfiI CCNNNNNNNGG 1 cut(s) 814
AgsI TTSAA 4 cut(s) 280, 352, 365, 777
AhlI ACTAGT 1 cut(s) 449
AjnI CCWGG 1 cut(s) 252
AluBI AGCT 6 cut(s) 156, 180, 377, 537, 798, 804
AluI AGCT 6 cut(s) 156, 180, 377, 537, 798, 804
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 2 cut(s) 98, 854
Ama87I CYCGRG 2 cut(s) 181, 862
AoxI GGCC 3 cut(s) 3, 235, 764
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 2 cut(s) 439, 563
AspLEI GCGC 3 cut(s) 122, 435, 601
AspS9I GGNCC 4 cut(s) 236, 328, 464, 765
AsuC2I CCSGG 1 cut(s) 462
AvaI CYCGRG 2 cut(s) 181, 862
AvaII GGWCC 2 cut(s) 328, 464
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 432
BanII GRGCYC 1 cut(s) 401
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 761
BccI CCATC 1 cut(s) 84
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 1 cut(s) 254
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 2 cut(s) 98, 854
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 5 cut(s) 378, 450, 524, 702, 783
BfmI CTRYAG 1 cut(s) 799
BfoI RGCGCY 2 cut(s) 436, 602
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 1 cut(s) 750
BlsI GCNGC 1 cut(s) 751
Bme1390I CCNGG 2 cut(s) 254, 462
Bme18I GGWCC 2 cut(s) 328, 464
BmeT110I CYCGRG 2 cut(s) 181, 862
BmgT120I GGNCC 4 cut(s) 236, 328, 464, 765
BmiI GGNNCC 4 cut(s) 15, 398, 434, 465
BmrFI CCNGG 2 cut(s) 254, 462
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 3 cut(s) 643, 721, 725
BmuI ACTGGG 1 cut(s) 200
BpmI CTGGAG 2 cut(s) 178, 609
Bpu10I CCTNAGC 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 854
BsaJI CCNNGG 1 cut(s) 507
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 1 cut(s) 814
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 2 cut(s) 206, 247
BseBI CCWGG 1 cut(s) 254
BseDI CCNNGG 1 cut(s) 507
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 1 cut(s) 814
BseMII CTCAG 3 cut(s) 375, 503, 806
BseNI ACTGG 2 cut(s) 206, 247
BseRI GAGGAG 3 cut(s) 10, 75, 78
BseXI GCAGC 1 cut(s) 761
BseYI CCCAGC 2 cut(s) 344, 373
BsgI GTGCAG 2 cut(s) 740, 768
Bsh1236I CGCG 2 cut(s) 120, 509
BshFI GGCC 3 cut(s) 5, 237, 766
BshNI GGYRCC 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 164
BsiHKCI CYCGRG 2 cut(s) 181, 862
BsiSI CCGG 3 cut(s) 146, 461, 744
BslFI GGGAC 1 cut(s) 842
BslI CCNNNNNNNGG 1 cut(s) 814
BsmAI GTCTC 2 cut(s) 98, 854
BsmBI CGTCTC 1 cut(s) 98
BsmFI GGGAC 1 cut(s) 842
BsnI GGCC 3 cut(s) 5, 237, 766
Bso31I GGTCTC 1 cut(s) 854
BsoBI CYCGRG 2 cut(s) 181, 862
Bsp1286I GDGCHC 2 cut(s) 164, 401
Bsp1407I TGTACA 1 cut(s) 836
BspACI CCGC 5 cut(s) 97, 221, 507, 509, 516
BspANI GGCC 3 cut(s) 5, 237, 766
BspCNI CTCAG 3 cut(s) 376, 502, 805
BspFNI CGCG 2 cut(s) 120, 509
BspLI GGNNCC 4 cut(s) 15, 398, 434, 465
BspQI GCTCTTC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 854
BsrFI RCCGGY 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 836
BsrI ACTGG 2 cut(s) 206, 247
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 1 cut(s) 507
BssNI GRCGYC 1 cut(s) 433
Bst2UI CCWGG 1 cut(s) 254
Bst4CI ACNGT 3 cut(s) 337, 630, 761
Bst6I CTCTTC 3 cut(s) 146, 468, 813
BstACI GRCGYC 1 cut(s) 433
BstAPI GCANNNNNTGC 1 cut(s) 740
BstAUI TGTACA 1 cut(s) 836
BstDEI CTNAG 4 cut(s) 384, 489, 792, 900
BstDSI CCRYGG 1 cut(s) 507
BstENI CCTNNNNNAGG 1 cut(s) 812
BstF5I GGATG 1 cut(s) 535
BstFNI CGCG 2 cut(s) 120, 509
BstH2I RGCGCY 2 cut(s) 436, 602
BstHHI GCGC 3 cut(s) 122, 435, 601
BstMAI GTCTC 2 cut(s) 98, 854
BstMWI GCNNNNNNNGC 7 cut(s) 11, 128, 210, 740, 749, 763, 788
BstNI CCWGG 1 cut(s) 254
BstSCI CCNGG 2 cut(s) 252, 460
BstSFI CTRYAG 1 cut(s) 799
BstUI CGCG 2 cut(s) 120, 509
BstV1I GCAGC 1 cut(s) 761
BsuRI GGCC 3 cut(s) 5, 237, 766
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 752
BtsIMutI CAGTG 3 cut(s) 635, 752, 757
CfoI GCGC 3 cut(s) 122, 435, 601
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 4 cut(s) 236, 328, 464, 765
Cfr42I CCGCGG 1 cut(s) 510
Csp6I GTAC 2 cut(s) 358, 837
CviAII CATG 4 cut(s) 201, 740, 843, 889
CviQI GTAC 2 cut(s) 358, 837
DdeI CTNAG 4 cut(s) 384, 489, 792, 900
DinI GGCGCC 1 cut(s) 434
DraI TTTAAA 2 cut(s) 481, 502
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 146, 468, 813
EarI CTCTTC 3 cut(s) 146, 468, 813
Eco24I GRGCYC 1 cut(s) 401
Eco31I GGTCTC 1 cut(s) 854
Eco47I GGWCC 2 cut(s) 328, 464
Eco88I CYCGRG 2 cut(s) 181, 862
EcoNI CCTNNNNNAGG 1 cut(s) 812
EcoO109I RGGNCCY 1 cut(s) 464
EcoRII CCWGG 1 cut(s) 252
EcoT38I GRGCYC 1 cut(s) 401
EgeI GGCGCC 1 cut(s) 434
EheI GGCGCC 1 cut(s) 434
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 4 cut(s) 204, 743, 846, 892
FaiI YATR 9 cut(s) 202, 216, 265, 420, 620, 636, 741, 844, 890
FaqI GGGAC 1 cut(s) 842
FatI CATG 4 cut(s) 200, 739, 842, 888
Fnu4HI GCNGC 1 cut(s) 750
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 1 cut(s) 750
FspBI CTAG 5 cut(s) 378, 450, 524, 702, 783
GlaI GCGC 3 cut(s) 121, 434, 600
GluI GCNGC 1 cut(s) 750
GsaI CCCAGC 2 cut(s) 348, 377
GsuI CTGGAG 2 cut(s) 178, 609
HaeII RGCGCY 2 cut(s) 436, 602
HaeIII GGCC 3 cut(s) 5, 237, 766
HapII CCGG 3 cut(s) 146, 461, 744
HhaI GCGC 3 cut(s) 122, 435, 601
Hin1I GRCGYC 1 cut(s) 433
Hin1II CATG 4 cut(s) 204, 743, 846, 892
Hin6I GCGC 3 cut(s) 120, 433, 599
HinP1I GCGC 3 cut(s) 120, 433, 599
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 1 cut(s) 673
HpaII CCGG 3 cut(s) 146, 461, 744
Hpy166II GTNNAC 2 cut(s) 370, 837
Hpy188I TCNGA 4 cut(s) 613, 626, 795, 903
Hpy188III TCNNGA 4 cut(s) 467, 491, 588, 853
Hpy8I GTNNAC 2 cut(s) 370, 837
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 506, 783
HpyCH4III ACNGT 3 cut(s) 337, 630, 761
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 6 cut(s) 131, 285, 716, 734, 749, 757
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 128, 210, 740, 749, 763, 788
HpyF3I CTNAG 4 cut(s) 384, 489, 792, 900
HpySE526I ACGT 1 cut(s) 360
Hsp92I GRCGYC 1 cut(s) 433
Hsp92II CATG 4 cut(s) 204, 743, 846, 892
HspAI GCGC 3 cut(s) 120, 433, 599
KasI GGCGCC 1 cut(s) 432
KspI CCGCGG 1 cut(s) 510
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 4 cut(s) 19, 159, 396, 627
Lsp1109I GCAGC 1 cut(s) 761
LweI GCATC 3 cut(s) 643, 721, 725
MaeI CTAG 5 cut(s) 378, 450, 524, 702, 783
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 293, 337
MboII GAAGA 3 cut(s) 163, 485, 830
MhlI GDGCHC 2 cut(s) 164, 401
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 70, 439, 563
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 433
MmeI TCCRAC 1 cut(s) 649
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 480, 501, 645
MslI CAYNNNNRTG 1 cut(s) 744
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 3 cut(s) 146, 461, 744
MspR9I CCNGG 2 cut(s) 254, 462
MvaI CCWGG 1 cut(s) 254
MvnI CGCG 2 cut(s) 120, 509
MwoI GCNNNNNNNGC 7 cut(s) 11, 128, 210, 740, 749, 763, 788
NarI GGCGCC 1 cut(s) 433
NciI CCSGG 1 cut(s) 462
NlaIII CATG 4 cut(s) 204, 743, 846, 892
NlaIV GGNNCC 4 cut(s) 15, 398, 434, 465
NmuCI GTSAC 1 cut(s) 293
PaeR7I CTCGAG 1 cut(s) 862
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 860
PfeI GAWTC 1 cut(s) 673
PkrI GCNGC 1 cut(s) 751
PluTI GGCGCC 1 cut(s) 436
PpuMI RGGWCCY 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 464
Psp6I CCWGG 1 cut(s) 252
PspFI CCCAGC 2 cut(s) 344, 373
PspGI CCWGG 1 cut(s) 252
PspN4I GGNNCC 4 cut(s) 15, 398, 434, 465
PspPI GGNCC 4 cut(s) 236, 328, 464, 765
PspPPI RGGWCCY 1 cut(s) 464
PspXI VCTCGAGB 1 cut(s) 862
PsrI GAACNNNNNNTAC 2 cut(s) 210, 242
RsaI GTAC 2 cut(s) 359, 838
RsaNI GTAC 2 cut(s) 358, 837
RseI CAYNNNNRTG 1 cut(s) 744
SacII CCGCGG 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 146
SaqAI TTAA 3 cut(s) 480, 501, 645
SatI GCNGC 1 cut(s) 750
Sau96I GGNCC 4 cut(s) 236, 328, 464, 765
ScrFI CCNGG 2 cut(s) 254, 462
SduI GDGCHC 2 cut(s) 164, 401
SfaNI GCATC 3 cut(s) 643, 721, 725
SfcI CTRYAG 1 cut(s) 799
SfoI GGCGCC 1 cut(s) 434
Sfr274I CTCGAG 1 cut(s) 862
Sfr303I CCGCGG 1 cut(s) 510
SgrBI CCGCGG 1 cut(s) 510
SinI GGWCC 2 cut(s) 328, 464
SlaI CTCGAG 1 cut(s) 862
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 1 cut(s) 862
SmoI CTYRAG 1 cut(s) 862
SpeI ACTAGT 1 cut(s) 449
Sse9I AATT 3 cut(s) 70, 439, 563
SsiI CCGC 5 cut(s) 97, 221, 507, 509, 516
SspDI GGCGCC 1 cut(s) 432
SspMI CTAG 5 cut(s) 378, 450, 524, 702, 783
StyD4I CCNGG 2 cut(s) 252, 460
TaaI ACNGT 3 cut(s) 337, 630, 761
TaiI ACGT 1 cut(s) 363
TaqI TCGA 3 cut(s) 557, 729, 863
TasI AATT 3 cut(s) 70, 439, 563
TatI WGTACW 1 cut(s) 836
TfiI GAWTC 1 cut(s) 673
Tru1I TTAA 3 cut(s) 480, 501, 645
Tru9I TTAA 3 cut(s) 480, 501, 645
TscAI CASTG 3 cut(s) 635, 759, 764
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 4 cut(s) 231, 435, 486, 874
TspGWI ACGGA 1 cut(s) 827
TspRI CASTG 3 cut(s) 635, 759, 764
VpaK11BI GGWCC 2 cut(s) 328, 464
XagI CCTNNNNNAGG 1 cut(s) 812
XapI RAATTY 2 cut(s) 439, 563
XhoI CTCGAG 1 cut(s) 862
XspI CTAG 5 cut(s) 378, 450, 524, 702, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.