MD15G1161400.v1.1

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0002821
Physical Location & Seq
Forward (+)
12126501 .. 12129674
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1161400.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGGCGACGACACCCTCCTCCTCCACTCTCCACCCTCTCCACCAAACATTTCTATCCCTAAGCTACCCTCCCCTCCATCACAATACTCAATTCCGCCCTCCCCATCTCTGCCTCACCTCCCGCCATTCCTCCCACCCCATTCGCGCCACCTCTACCGTCACTCTCCAACCGGAGCGGCGGTCGACCGCCGAGGAAGGTCAAAGCTCCGGCGTTGACCTCGAGCTGCTAGCCTGCCCAGTATGCTACGAGCCGCTCATCCGGAAAGGCCCGCCGGGTCTCAACCTGCGAGCAATTTACAGGTCGGGGTTCAAGTGCAGGAAATGCGACAAGTCGTATTCAAGCAAAGACATTTACTTGGACCTTACTGTTACTGCTGGGTTGAAGGAGTACGTTGAGGTAAAACCGGCCGGGACCGAGTTGTTCCGGAGCCCTATTGTTTCCTTCTTGTATGAAAGGGGTTGGCGCCAGAATTTTAACCGCAGGGGATTTCCGGGTCCCGATGAAGAGTTTAAGATGGCTCAGGACTACTTTAAATCCGCCGAAGGCGGTGTTTTGGTGGATGTGAGCTGTGGCAGCGGCTTGTTTTCGAGGAAGTTTGCGAAATCGGGGACATATTCTGGAGTTGTTGCACTTGATTTTTCGGAGAACATGCTTCGGCAGAGTTATGAATTTTTTAAGAGAGATCCTACTCTTTTAGATACTAATCTTGCCCTTGTGAGGGCGGATGTTGCTAGGCTTCCGTTCCCTTCGGGTTCAGTTGATGCTGTCCATGCTGGCGCAGCATTACATTGTTGGCCTTCTCCTTCCAATGCCATTGCTGAAATAACTCGCATATTACGAAGTGGTGCTGTCTTTGTCGGAACCACTTTTCTGCGGTATACTCCATCTACTTCCTGGATACCCCGGCAACTCATAGAGAGGGCTCGTCAGAGCTATAACTATTTAACGGAGGAGGAGATTGCAGACTTGTGTACATCATGCGGTCTCACCAACTACACGAGCAACGTTCAACAATCCTTCATCATGTTTTCTGCTCAGAAACCTTAA

Protein Analysis

349

Amino Acids

38.82

Weight (kDa)

8.79

Isoelectric Point (pI)

53.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 176 - 288 1.6e-09 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 179 - 332 9.9e-11 Methyltransferase domain
Methyltransf_31 PF13847 182 - 301 7.5e-11 Methyltransferase domain
Methyltransf_11 PF08241 186 - 286 2.4e-19 Methyltransferase domain
Methyltransf_25 PF13649 186 - 282 8.2e-16 Methyltransferase domain
Methyltransf_12 PF08242 186 - 285 1.4e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 291
AccB1I GGYRCC 1 cut(s) 462
AccBSI CCGCTC 2 cut(s) 175, 253
AccI GTMKAC 2 cut(s) 182, 878
AccII CGCG 1 cut(s) 144
AccIII TCCGGA 2 cut(s) 258, 423
AclI AACGTT 1 cut(s) 1005
AclWI GGATC 1 cut(s) 677
AcoI YGGCCR 1 cut(s) 405
AcsI RAATTY 2 cut(s) 469, 668
AcyI GRCGYC 1 cut(s) 463
AfaI GTAC 2 cut(s) 389, 973
AfiI CCNNNNNNNGG 2 cut(s) 717, 718
AgsI TTSAA 4 cut(s) 310, 339, 382, 1010
AjnI CCWGG 1 cut(s) 893
AluBI AGCT 5 cut(s) 63, 204, 223, 567, 933
AluI AGCT 5 cut(s) 63, 204, 223, 567, 933
Alw26I GTCTC 2 cut(s) 281, 989
AlwI GGATC 1 cut(s) 677
Ama87I CYCGRG 1 cut(s) 218
Aor13HI TCCGGA 2 cut(s) 258, 423
AoxI GGCC 3 cut(s) 265, 405, 794
ApeKI GCWGC 3 cut(s) 223, 573, 779
ApoI RAATTY 2 cut(s) 469, 668
AspLEI GCGC 3 cut(s) 146, 465, 779
AspS9I GGNCC 4 cut(s) 266, 358, 411, 494
AsuC2I CCSGG 4 cut(s) 273, 409, 492, 904
AsuHPI GGTGA 2 cut(s) 106, 979
AsuNHI GCTAGC 1 cut(s) 226
AvaI CYCGRG 1 cut(s) 218
AvaII GGWCC 3 cut(s) 358, 411, 494
BanI GGYRCC 1 cut(s) 462
BanII GRGCYC 2 cut(s) 431, 925
BauI CACGAG 1 cut(s) 997
BbvI GCAGC 3 cut(s) 210, 585, 791
BccI CCATC 4 cut(s) 84, 111, 508, 892
BciT130I CCWGG 1 cut(s) 895
BciVI GTATCC 1 cut(s) 891
BcnI CCSGG 4 cut(s) 273, 409, 492, 904
BcoDI GTCTC 2 cut(s) 281, 989
BfaI CTAG 2 cut(s) 227, 732
BfoI RGCGCY 1 cut(s) 466
BfuAI ACCTGC 1 cut(s) 291
BfuI GTATCC 1 cut(s) 891
BisI GCNGC 6 cut(s) 176, 224, 251, 574, 577, 780
BlsI GCNGC 6 cut(s) 177, 225, 252, 575, 578, 781
Bme1390I CCNGG 5 cut(s) 273, 409, 492, 895, 904
Bme18I GGWCC 3 cut(s) 358, 411, 494
BmeT110I CYCGRG 1 cut(s) 218
BmgT120I GGNCC 4 cut(s) 266, 358, 411, 494
BmiI GGNNCC 6 cut(s) 412, 428, 464, 495, 496, 862
BmrFI CCNGG 5 cut(s) 273, 409, 492, 895, 904
BmrI ACTGGG 1 cut(s) 230
BmsI GCATC 1 cut(s) 751
BmtI GCTAGC 1 cut(s) 230
BmuI ACTGGG 1 cut(s) 230
BpmI CTGGAG 1 cut(s) 639
Bpu10I CCTNAGC 2 cut(s) 59, 519
BpuMI CCSGG 4 cut(s) 273, 409, 492, 904
BsaBI GATNNNNATC 1 cut(s) 702
BsaHI GRCGYC 1 cut(s) 463
BsaI GGTCTC 2 cut(s) 281, 989
BsaJI CCNNGG 2 cut(s) 189, 902
BsaWI WCCGGW 3 cut(s) 169, 258, 423
BsaXI ACNNNNNCTCC 2 cut(s) 164, 194
Bsc4I CCNNNNNNNGG 2 cut(s) 717, 718
Bse118I RCCGGY 1 cut(s) 403
Bse1I ACTGG 1 cut(s) 236
Bse3DI GCAATG 1 cut(s) 813
Bse8I GATNNNNATC 1 cut(s) 702
BseAI TCCGGA 2 cut(s) 258, 423
BseBI CCWGG 1 cut(s) 895
BseDI CCNNGG 2 cut(s) 189, 902
BseGI GGATG 3 cut(s) 255, 565, 730
BseJI GATNNNNATC 1 cut(s) 702
BseLI CCNNNNNNNGG 2 cut(s) 717, 718
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 1 cut(s) 533
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 4 cut(s) 7, 10, 965, 968
BseX3I CGGCCG 1 cut(s) 405
BseXI GCAGC 3 cut(s) 210, 585, 791
BseYI CCCAGC 1 cut(s) 374
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 3 cut(s) 182, 186, 408
BshFI GGCC 3 cut(s) 267, 407, 796
BshNI GGYRCC 1 cut(s) 462
BsiEI CGRYCG 3 cut(s) 182, 186, 408
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 9 cut(s) 170, 207, 259, 272, 404, 408, 424, 491, 904
BslFI GGGAC 3 cut(s) 424, 480, 622
BslI CCNNNNNNNGG 2 cut(s) 717, 718
BsmAI GTCTC 2 cut(s) 281, 989
BsmFI GGGAC 3 cut(s) 424, 480, 622
BsnI GGCC 3 cut(s) 267, 407, 796
Bso31I GGTCTC 2 cut(s) 281, 989
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 2 cut(s) 431, 925
Bsp13I TCCGGA 2 cut(s) 258, 423
Bsp1407I TGTACA 1 cut(s) 971
Bsp143I GATC 1 cut(s) 682
BspANI GGCC 3 cut(s) 267, 407, 796
BspCNI CTCAG 1 cut(s) 532
BspEI TCCGGA 2 cut(s) 258, 423
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 6 cut(s) 412, 428, 464, 495, 496, 862
BspMI ACCTGC 1 cut(s) 291
BspOI GCTAGC 1 cut(s) 230
BspPI GGATC 1 cut(s) 677
BspT107I GGYRCC 1 cut(s) 462
BspTNI GGTCTC 2 cut(s) 281, 989
BsrBI CCGCTC 2 cut(s) 175, 253
BsrDI GCAATG 1 cut(s) 813
BsrFI RCCGGY 1 cut(s) 403
BsrGI TGTACA 1 cut(s) 971
BsrI ACTGG 1 cut(s) 236
BssAI RCCGGY 1 cut(s) 403
BssECI CCNNGG 2 cut(s) 189, 902
BssMI GATC 1 cut(s) 682
BssNAI GTATAC 1 cut(s) 879
BssNI GRCGYC 1 cut(s) 463
BssSI CACGAG 1 cut(s) 997
Bst1107I GTATAC 1 cut(s) 879
Bst2BI CACGAG 1 cut(s) 997
Bst2UI CCWGG 1 cut(s) 895
Bst4CI ACNGT 2 cut(s) 157, 367
Bst6I CTCTTC 1 cut(s) 498
BstACI GRCGYC 1 cut(s) 463
BstAPI GCANNNNNTGC 1 cut(s) 321
BstAUI TGTACA 1 cut(s) 971
BstC8I GCNNGC 5 cut(s) 228, 232, 269, 288, 775
BstDEI CTNAG 3 cut(s) 59, 519, 1035
BstF5I GGATG 3 cut(s) 255, 565, 730
BstFNI CGCG 1 cut(s) 144
BstH2I RGCGCY 1 cut(s) 466
BstHHI GCGC 3 cut(s) 146, 465, 779
BstKTI GATC 1 cut(s) 685
BstMAI GTCTC 2 cut(s) 281, 989
BstMBI GATC 1 cut(s) 682
BstMCI CGRYCG 3 cut(s) 182, 186, 408
BstMWI GCNNNNNNNGC 6 cut(s) 240, 321, 573, 728, 770, 779
BstNI CCWGG 1 cut(s) 895
BstNSI RCATGY 1 cut(s) 652
BstSCI CCNGG 5 cut(s) 271, 407, 490, 893, 902
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 3 cut(s) 210, 585, 791
BstX2I RGATCY 1 cut(s) 682
BstYI RGATCY 1 cut(s) 682
BstZ17I GTATAC 1 cut(s) 879
BstZI CGGCCG 1 cut(s) 405
BsuI GTATCC 1 cut(s) 891
BsuRI GGCC 3 cut(s) 267, 407, 796
BtsCI GGATG 3 cut(s) 255, 565, 730
BveI ACCTGC 1 cut(s) 291
Cac8I GCNNGC 5 cut(s) 228, 232, 269, 288, 775
CfoI GCGC 3 cut(s) 146, 465, 779
Cfr10I RCCGGY 1 cut(s) 403
Cfr13I GGNCC 4 cut(s) 266, 358, 411, 494
Csp6I GTAC 2 cut(s) 388, 972
CviAII CATG 4 cut(s) 649, 770, 978, 1024
CviQI GTAC 2 cut(s) 388, 972
DdeI CTNAG 3 cut(s) 59, 519, 1035
DinI GGCGCC 1 cut(s) 464
DpnI GATC 1 cut(s) 684
DpnII GATC 1 cut(s) 682
DraI TTTAAA 1 cut(s) 532
EaeI YGGCCR 1 cut(s) 405
EagI CGGCCG 1 cut(s) 405
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 3 cut(s) 83, 526, 737
EclXI CGGCCG 1 cut(s) 405
Eco24I GRGCYC 2 cut(s) 431, 925
Eco31I GGTCTC 2 cut(s) 281, 989
Eco47I GGWCC 3 cut(s) 358, 411, 494
Eco52I CGGCCG 1 cut(s) 405
Eco88I CYCGRG 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 494
EcoRII CCWGG 1 cut(s) 893
EcoT38I GRGCYC 2 cut(s) 431, 925
EgeI GGCGCC 1 cut(s) 464
EheI GGCGCC 1 cut(s) 464
FaeI CATG 4 cut(s) 652, 773, 981, 1027
FaqI GGGAC 3 cut(s) 424, 480, 622
FatI CATG 4 cut(s) 648, 769, 977, 1023
FauI CCCGC 2 cut(s) 128, 276
FblI GTMKAC 2 cut(s) 182, 878
Fnu4HI GCNGC 6 cut(s) 176, 224, 251, 574, 577, 780
FokI GGATG 3 cut(s) 242, 572, 737
FriOI GRGCYC 2 cut(s) 431, 925
Fsp4HI GCNGC 6 cut(s) 176, 224, 251, 574, 577, 780
FspBI CTAG 2 cut(s) 227, 732
GlaI GCGC 3 cut(s) 145, 464, 778
GluI GCNGC 6 cut(s) 176, 224, 251, 574, 577, 780
GsaI CCCAGC 1 cut(s) 378
GsuI CTGGAG 1 cut(s) 639
HaeII RGCGCY 1 cut(s) 466
HaeIII GGCC 3 cut(s) 267, 407, 796
HapII CCGG 9 cut(s) 170, 207, 259, 272, 404, 408, 424, 491, 904
HhaI GCGC 3 cut(s) 146, 465, 779
Hin1I GRCGYC 1 cut(s) 463
Hin1II CATG 4 cut(s) 652, 773, 981, 1027
Hin6I GCGC 3 cut(s) 144, 463, 777
HinP1I GCGC 3 cut(s) 144, 463, 777
HincII GTYRAC 2 cut(s) 183, 214
HindII GTYRAC 2 cut(s) 183, 214
HpaII CCGG 9 cut(s) 170, 207, 259, 272, 404, 408, 424, 491, 904
HphI GGTGA 2 cut(s) 106, 979
Hpy166II GTNNAC 4 cut(s) 183, 214, 879, 972
Hpy188I TCNGA 4 cut(s) 643, 860, 930, 1038
Hpy188III TCNNGA 5 cut(s) 259, 424, 497, 521, 618
Hpy8I GTNNAC 4 cut(s) 183, 214, 879, 972
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 8 cut(s) 188, 376, 451, 536, 756, 807, 813, 1027
HpyCH4III ACNGT 2 cut(s) 157, 367
HpyCH4IV ACGT 2 cut(s) 390, 1005
HpyCH4V TGCA 3 cut(s) 315, 629, 962
HpyF10VI GCNNNNNNNGC 6 cut(s) 240, 321, 573, 728, 770, 779
HpyF3I CTNAG 3 cut(s) 59, 519, 1035
HpySE526I ACGT 2 cut(s) 390, 1005
Hsp92I GRCGYC 1 cut(s) 463
Hsp92II CATG 4 cut(s) 652, 773, 981, 1027
HspAI GCGC 3 cut(s) 144, 463, 777
KasI GGCGCC 1 cut(s) 462
KflI GGGWCCC 1 cut(s) 494
Kpn2I TCCGGA 2 cut(s) 258, 423
Kzo9I GATC 1 cut(s) 682
LmnI GCTCC 3 cut(s) 172, 209, 426
Lsp1109I GCAGC 3 cut(s) 210, 585, 791
LweI GCATC 1 cut(s) 751
MaeI CTAG 2 cut(s) 227, 732
MaeII ACGT 2 cut(s) 390, 1005
MaeIII GTNAC 2 cut(s) 157, 367
MalI GATC 1 cut(s) 684
MbiI CCGCTC 2 cut(s) 175, 253
MboI GATC 1 cut(s) 682
MboII GAAGA 1 cut(s) 515
MflI RGATCY 1 cut(s) 682
MhlI GDGCHC 2 cut(s) 431, 925
MluCI AATT 4 cut(s) 89, 291, 469, 668
Mly113I GGCGCC 1 cut(s) 463
MmeI TCCRAC 2 cut(s) 190, 838
MroI TCCGGA 2 cut(s) 258, 423
MseI TTAA 6 cut(s) 474, 510, 531, 675, 944, 1045
MspA1I CMGCKG 1 cut(s) 576
MspI CCGG 9 cut(s) 170, 207, 259, 272, 404, 408, 424, 491, 904
MspR9I CCNGG 5 cut(s) 273, 409, 492, 895, 904
MvaI CCWGG 1 cut(s) 895
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 6 cut(s) 240, 321, 573, 728, 770, 779
NarI GGCGCC 1 cut(s) 463
NciI CCSGG 4 cut(s) 273, 409, 492, 904
NdeII GATC 1 cut(s) 682
NheI GCTAGC 1 cut(s) 226
NlaIII CATG 4 cut(s) 652, 773, 981, 1027
NlaIV GGNNCC 6 cut(s) 412, 428, 464, 495, 496, 862
NmeAIII GCCGAG 1 cut(s) 214
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 652
PaeR7I CTCGAG 1 cut(s) 218
PcsI WCGNNNNNNNCGW 1 cut(s) 835
PfoI TCCNGGA 1 cut(s) 893
PkrI GCNGC 6 cut(s) 177, 225, 252, 575, 578, 781
PluTI GGCGCC 1 cut(s) 466
PpuMI RGGWCCY 1 cut(s) 494
Psp1406I AACGTT 1 cut(s) 1005
Psp5II RGGWCCY 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 893
PspFI CCCAGC 1 cut(s) 374
PspGI CCWGG 1 cut(s) 893
PspN4I GGNNCC 6 cut(s) 412, 428, 464, 495, 496, 862
PspPI GGNCC 4 cut(s) 266, 358, 411, 494
PspPPI RGGWCCY 1 cut(s) 494
PspXI VCTCGAGB 1 cut(s) 218
PsuI RGATCY 1 cut(s) 682
RsaI GTAC 2 cut(s) 389, 973
RsaNI GTAC 2 cut(s) 388, 972
SalI GTCGAC 1 cut(s) 181
SaqAI TTAA 6 cut(s) 474, 510, 531, 675, 944, 1045
SatI GCNGC 6 cut(s) 176, 224, 251, 574, 577, 780
Sau3AI GATC 1 cut(s) 682
Sau96I GGNCC 4 cut(s) 266, 358, 411, 494
ScrFI CCNGG 5 cut(s) 273, 409, 492, 895, 904
SduI GDGCHC 2 cut(s) 431, 925
SfaNI GCATC 1 cut(s) 751
SfoI GGCGCC 1 cut(s) 464
Sfr274I CTCGAG 1 cut(s) 218
SinI GGWCC 3 cut(s) 358, 411, 494
SlaI CTCGAG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 218
SmoI CTYRAG 1 cut(s) 218
Sse9I AATT 4 cut(s) 89, 291, 469, 668
SspDI GGCGCC 1 cut(s) 462
SspMI CTAG 2 cut(s) 227, 732
StyD4I CCNGG 5 cut(s) 271, 407, 490, 893, 902
TaaI ACNGT 2 cut(s) 157, 367
TaiI ACGT 2 cut(s) 393, 1008
TaqI TCGA 3 cut(s) 182, 219, 587
TaqII GACCGA 1 cut(s) 428
TasI AATT 4 cut(s) 89, 291, 469, 668
TatI WGTACW 1 cut(s) 971
TauI GCSGC 3 cut(s) 178, 253, 579
Tru1I TTAA 6 cut(s) 474, 510, 531, 675, 944, 1045
Tru9I TTAA 6 cut(s) 474, 510, 531, 675, 944, 1045
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 3 cut(s) 223, 573, 779
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 4 cut(s) 465, 516, 681, 1009
TspGWI ACGGA 2 cut(s) 729, 962
VpaK11BI GGWCC 3 cut(s) 358, 411, 494
XapI RAATTY 2 cut(s) 469, 668
XceI RCATGY 1 cut(s) 652
XcmI CCANNNNNNNNNTGG 1 cut(s) 891
XhoI CTCGAG 1 cut(s) 218
XmiI GTMKAC 2 cut(s) 182, 878
XspI CTAG 2 cut(s) 227, 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.