RLG00000015812

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002821
Physical Location & Seq
Forward (+)
1589879 .. 1591321
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015812

Sequence Viewer

Length: 768 bp
ATGGGAACGGCTCTCTCATCTTTCAATGAATCGAGTCAAAGCTCGGAGATTGAGTTGTTATCCTGCCCGGTATGCTATGAACCACTAACACGAAAAGGCCCACCTGGTCTAATCCTGCAAACAATATGCAGGTCATCGTTCGAGTGCAAGAAATGCTACAAGTCGTGTTCCAGTAAACACAACTACTTGGATCTCACTGTTACTGCTGTATTGAAGAAGTACGTTGAAGTTAAACCAGTTGGCACTGAGTTATTCAGGAGATCCTTCATGTCCTTCATCTATGAAAGAGGTTATCGTCAGGGTTTTAAACGATATGGCTTCCCAGGTCCTGATGAAGAGGACTACTTTAAATCGGCTGAAGGTGGTGTTCTAGTGGATGTTAGCTGTGGGAGTGGTTTGTTCGCGATAAGATTTGCCAAATCTGGGACCTATTCTGGAGTTGTGGCACTTGATTTTTCTAATCTCGTTCTTGTGAGGGGCGATGTTTCTAGGCTTCCGTTCACATCGAGTTCAGTTGATGCTGTCCATGCCGGCGCTGCATTGCACTGCTGGCCCTCCCCTTCCAATGCTCTGCTTGAAATTAATAAATCACCAGTAACTCAATTCTCATATATTTATCAGGTTGCTGAAATCACTCGTGTATTACGAAGTGGTGGCATCTTTGCTGGAACCACTTTTCTGCGGTCTAGTCCTTCAGGTACTTCCTGGATATTCAAACCACTCAATGAGGATGGAGAGCTCTTGGTGTTTTACTATTATGAGCTTTGA

Protein Analysis

256

Amino Acids

28.2

Weight (kDa)

8.05

Isoelectric Point (pI)

58.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_11 PF08241 125 - 195 6.5e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 120
AccII CGCG 1 cut(s) 404
AciI CCGC 1 cut(s) 682
AclWI GGATC 2 cut(s) 198, 255
AcuI CTGAAG 2 cut(s) 378, 678
AfaI GTAC 2 cut(s) 221, 700
AfiI CCNNNNNNNGG 1 cut(s) 423
AgsI TTSAA 5 cut(s) 25, 214, 227, 578, 715
AjnI CCWGG 3 cut(s) 103, 322, 704
AluBI AGCT 4 cut(s) 42, 384, 739, 763
AluI AGCT 4 cut(s) 42, 384, 739, 763
Alw21I GWGCWC 1 cut(s) 741
AlwI GGATC 2 cut(s) 198, 255
AlwNI CAGNNNCTG 1 cut(s) 329
AoxI GGCC 2 cut(s) 97, 551
ApeKI GCWGC 1 cut(s) 536
AseI ATTAAT 1 cut(s) 582
AspLEI GCGC 1 cut(s) 536
AspS9I GGNCC 4 cut(s) 98, 326, 426, 552
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 582
AvaII GGWCC 2 cut(s) 326, 426
BanII GRGCYC 1 cut(s) 741
BauI CACGAG 1 cut(s) 636
Bbv12I GWGCWC 1 cut(s) 741
BbvI GCAGC 1 cut(s) 523
BccI CCATC 1 cut(s) 725
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 3 cut(s) 105, 324, 706
BcnI CCSGG 1 cut(s) 68
BfaI CTAG 3 cut(s) 371, 489, 687
BfoI RGCGCY 1 cut(s) 537
BfuAI ACCTGC 1 cut(s) 120
BisI GCNGC 1 cut(s) 537
BlsI GCNGC 1 cut(s) 538
Bme1390I CCNGG 4 cut(s) 68, 105, 324, 706
Bme18I GGWCC 2 cut(s) 326, 426
BmgT120I GGNCC 4 cut(s) 98, 326, 426, 552
BmiI GGNNCC 2 cut(s) 427, 670
BmrFI CCNGG 4 cut(s) 68, 105, 324, 706
BmsI GCATC 2 cut(s) 508, 666
BpmI CTGGAG 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 68
BsaJI CCNNGG 1 cut(s) 322
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bsc4I CCNNNNNNNGG 1 cut(s) 423
Bse118I RCCGGY 1 cut(s) 530
Bse1I ACTGG 3 cut(s) 171, 236, 593
Bse3DI GCAATG 1 cut(s) 539
BseBI CCWGG 3 cut(s) 105, 324, 706
BseDI CCNNGG 1 cut(s) 322
BseGI GGATG 2 cut(s) 382, 736
BseLI CCNNNNNNNGG 1 cut(s) 423
BseMI GCAATG 1 cut(s) 539
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 171, 236, 593
BseXI GCAGC 1 cut(s) 523
Bsh1236I CGCG 1 cut(s) 404
BshFI GGCC 2 cut(s) 99, 553
BsiHKAI GWGCWC 1 cut(s) 741
BsiSI CCGG 2 cut(s) 68, 531
BslFI GGGAC 1 cut(s) 439
BslI CCNNNNNNNGG 1 cut(s) 423
BsmFI GGGAC 1 cut(s) 439
BsnI GGCC 2 cut(s) 99, 553
Bsp1286I GDGCHC 1 cut(s) 741
Bsp143I GATC 2 cut(s) 190, 260
Bsp68I TCGCGA 1 cut(s) 404
BspACI CCGC 1 cut(s) 682
BspANI GGCC 2 cut(s) 99, 553
BspCNI CTCAG 1 cut(s) 238
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 2 cut(s) 427, 670
BspMI ACCTGC 1 cut(s) 120
BspPI GGATC 2 cut(s) 198, 255
BsrDI GCAATG 1 cut(s) 539
BsrFI RCCGGY 1 cut(s) 530
BsrI ACTGG 3 cut(s) 171, 236, 593
BssAI RCCGGY 1 cut(s) 530
BssECI CCNNGG 1 cut(s) 322
BssMI GATC 2 cut(s) 190, 260
BssSI CACGAG 1 cut(s) 636
Bst2BI CACGAG 1 cut(s) 636
Bst2UI CCWGG 3 cut(s) 105, 324, 706
Bst4CI ACNGT 1 cut(s) 199
Bst6I CTCTTC 1 cut(s) 330
BstAPI GCANNNNNTGC 1 cut(s) 153
BstC8I GCNNGC 2 cut(s) 532, 551
BstDEI CTNAG 1 cut(s) 246
BstF5I GGATG 2 cut(s) 382, 736
BstFNI CGCG 1 cut(s) 404
BstH2I RGCGCY 1 cut(s) 537
BstHHI GCGC 1 cut(s) 536
BstKTI GATC 2 cut(s) 193, 263
BstMBI GATC 2 cut(s) 190, 260
BstMWI GCNNNNNNNGC 5 cut(s) 72, 153, 527, 536, 550
BstNI CCWGG 3 cut(s) 105, 324, 706
BstSCI CCNGG 4 cut(s) 66, 103, 322, 704
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 523
BstX2I RGATCY 2 cut(s) 190, 260
BstYI RGATCY 2 cut(s) 190, 260
BsuRI GGCC 2 cut(s) 99, 553
BtgZI GCGATG 1 cut(s) 495
BtsCI GGATG 2 cut(s) 382, 736
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 3 cut(s) 195, 243, 544
BtuMI TCGCGA 1 cut(s) 404
BveI ACCTGC 1 cut(s) 120
Cac8I GCNNGC 2 cut(s) 532, 551
CaiI CAGNNNCTG 1 cut(s) 329
CfoI GCGC 1 cut(s) 536
Cfr10I RCCGGY 1 cut(s) 530
Cfr13I GGNCC 4 cut(s) 98, 326, 426, 552
CsiI ACCWGGT 1 cut(s) 103
Csp6I GTAC 2 cut(s) 220, 699
CviAII CATG 2 cut(s) 268, 527
CviQI GTAC 2 cut(s) 220, 699
DdeI CTNAG 1 cut(s) 246
DpnI GATC 2 cut(s) 192, 262
DpnII GATC 2 cut(s) 190, 260
DraI TTTAAA 2 cut(s) 307, 349
Eam1104I CTCTTC 1 cut(s) 330
EarI CTCTTC 1 cut(s) 330
Ecl136II GAGCTC 1 cut(s) 739
Eco24I GRGCYC 1 cut(s) 741
Eco47I GGWCC 2 cut(s) 326, 426
Eco53kI GAGCTC 1 cut(s) 739
Eco57I CTGAAG 2 cut(s) 378, 678
EcoICRI GAGCTC 1 cut(s) 739
EcoO109I RGGNCCY 2 cut(s) 326, 426
EcoRII CCWGG 3 cut(s) 103, 322, 704
EcoT38I GRGCYC 1 cut(s) 741
FaeI CATG 2 cut(s) 271, 530
FaqI GGGAC 1 cut(s) 439
FatI CATG 2 cut(s) 267, 526
Fnu4HI GCNGC 1 cut(s) 537
FokI GGATG 2 cut(s) 389, 743
FriOI GRGCYC 1 cut(s) 741
Fsp4HI GCNGC 1 cut(s) 537
FspBI CTAG 3 cut(s) 371, 489, 687
GlaI GCGC 1 cut(s) 535
GluI GCNGC 1 cut(s) 537
GsuI CTGGAG 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 537
HaeIII GGCC 2 cut(s) 99, 553
HapII CCGG 2 cut(s) 68, 531
HhaI GCGC 1 cut(s) 536
Hin1II CATG 2 cut(s) 271, 530
Hin6I GCGC 1 cut(s) 534
HinP1I GCGC 1 cut(s) 534
HinfI GANTC 2 cut(s) 29, 34
HpaII CCGG 2 cut(s) 68, 531
HphI GGTGA 1 cut(s) 582
Hpy166II GTNNAC 2 cut(s) 176, 501
Hpy188I TCNGA 1 cut(s) 46
Hpy188III TCNNGA 4 cut(s) 256, 329, 403, 435
Hpy8I GTNNAC 2 cut(s) 176, 501
HpyAV CCTTC 5 cut(s) 274, 283, 353, 570, 702
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 118, 129, 147, 539, 544
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 153, 527, 536, 550
HpyF3I CTNAG 1 cut(s) 246
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 2 cut(s) 271, 530
HspAI GCGC 1 cut(s) 534
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Kzo9I GATC 2 cut(s) 190, 260
Lsp1109I GCAGC 1 cut(s) 523
LweI GCATC 2 cut(s) 508, 666
MabI ACCWGGT 1 cut(s) 103
MaeI CTAG 3 cut(s) 371, 489, 687
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 199, 595
MalI GATC 2 cut(s) 192, 262
MboI GATC 2 cut(s) 190, 260
MboII GAAGA 2 cut(s) 226, 347
MflI RGATCY 2 cut(s) 190, 260
MhlI GDGCHC 1 cut(s) 741
MluCI AATT 2 cut(s) 579, 602
MlyI GAGTC 1 cut(s) 43
MnlI CCTC 5 cut(s) 281, 331, 468, 565, 721
MroNI GCCGGC 1 cut(s) 530
MseI TTAA 4 cut(s) 231, 306, 348, 582
MspI CCGG 2 cut(s) 68, 531
MspR9I CCNGG 4 cut(s) 68, 105, 324, 706
MvaI CCWGG 3 cut(s) 105, 324, 706
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 5 cut(s) 72, 153, 527, 536, 550
NaeI GCCGGC 1 cut(s) 532
NciI CCSGG 1 cut(s) 68
NdeII GATC 2 cut(s) 190, 260
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 2 cut(s) 271, 530
NlaIV GGNNCC 2 cut(s) 427, 670
NruI TCGCGA 1 cut(s) 404
PcsI WCGNNNNNNNCGW 1 cut(s) 643
PdiI GCCGGC 1 cut(s) 532
PfeI GAWTC 1 cut(s) 29
PfoI TCCNGGA 1 cut(s) 704
PkrI GCNGC 1 cut(s) 538
PleI GAGTC 1 cut(s) 42
PpsI GAGTC 1 cut(s) 42
PpuMI RGGWCCY 2 cut(s) 326, 426
PshBI ATTAAT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 741
Psp5II RGGWCCY 2 cut(s) 326, 426
Psp6I CCWGG 3 cut(s) 103, 322, 704
PspGI CCWGG 3 cut(s) 103, 322, 704
PspN4I GGNNCC 2 cut(s) 427, 670
PspPI GGNCC 4 cut(s) 98, 326, 426, 552
PspPPI RGGWCCY 2 cut(s) 326, 426
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 2 cut(s) 190, 260
RruI TCGCGA 1 cut(s) 404
RsaI GTAC 2 cut(s) 221, 700
RsaNI GTAC 2 cut(s) 220, 699
SacI GAGCTC 1 cut(s) 741
SaqAI TTAA 4 cut(s) 231, 306, 348, 582
SatI GCNGC 1 cut(s) 537
Sau3AI GATC 2 cut(s) 190, 260
Sau96I GGNCC 4 cut(s) 98, 326, 426, 552
SchI GAGTC 1 cut(s) 43
ScrFI CCNGG 4 cut(s) 68, 105, 324, 706
SduI GDGCHC 1 cut(s) 741
SexAI ACCWGGT 1 cut(s) 103
SfaNI GCATC 2 cut(s) 508, 666
SinI GGWCC 2 cut(s) 326, 426
Sse9I AATT 2 cut(s) 579, 602
SsiI CCGC 1 cut(s) 682
SspMI CTAG 3 cut(s) 371, 489, 687
SstI GAGCTC 1 cut(s) 741
StyD4I CCNGG 4 cut(s) 66, 103, 322, 704
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 32, 141, 506
TasI AATT 2 cut(s) 579, 602
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 4 cut(s) 231, 306, 348, 582
Tru9I TTAA 4 cut(s) 231, 306, 348, 582
TscAI CASTG 3 cut(s) 202, 250, 551
TseI GCWGC 1 cut(s) 536
TspDTI ATGAA 6 cut(s) 42, 93, 256, 265, 297, 348
TspGWI ACGGA 1 cut(s) 486
TspRI CASTG 3 cut(s) 202, 250, 551
VpaK11BI GGWCC 2 cut(s) 326, 426
VspI ATTAAT 1 cut(s) 582
XspI CTAG 3 cut(s) 371, 489, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.