Rw2G001840

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002821
Physical Location & Seq
Forward (+)
1516709 .. 1519480
2772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001840.1

Sequence Viewer

Length: 1035 bp
ATGGCCACGACGGCTCCCTCCTCCCTCCACCAAACATTTCTATCCCCAAAACACCCTCGCCTCCACCACAATTCCCATCTCTCTCCTCCTCGTCTCCGCTTCCACTCTCTCCCCATTCGCGCCACCTCTGCAATCACTCTACAACCGGAGAAGAGCTGGAGCACCCAGAAAAGTCAAAGCTCGGGCATTGAGTTTTTGTCATGCCCAGTTTGCTATGAACCGCTAATACGAAAAGGCCCAACTGGTCTAAACCTGGAAGCAATATACAGGTCAGGTTTCAAGTGCAAGAAATGTGACAAGTCGTTTTCCAGCAAAGACATTTACTTGGACCTTACTGTTACTGCTGGGTTGAAGCAGTACGTTGAAGTCAACCCAGCTAGGACTGAGTTGTTTAGGAGCCCACTTGTGTCGTTTTTGTATGAAAGAGGTTGGCGCCAGAATTTTAGCACTAGTGGATTTCCGGGTCCTGATGAAGAGTTTAAAATGGCTCAGGACTACTTTAAATCCGCGGAAGGCGGTTTTCTAGTGGATGTGAGCTGTGGGAGTGGTTTGTTTTCGAGGAAATTTGCGAAATCGGGGAGTTATTCTGGAGTTGTGGCGCTTGATTTTTCGGAAAATATGCTCCGACAGTGCTATGATTTTCTTAAAAGAGATGCTACTCTTTTGGCTACGAATCTTGCTCTTGTAAGGGCAGATGTTTCTAGGCTTCCATTTGCATCAGGTTCAGTCGATGCAGTCCATGCCGGTGCAGCACTGCACTGTTGGCCCTCTCCTTCAAATGCTGTTGCTGAAATCACTCGTGTATTACGAAGTGGTGGTGTCTTTGTTGGAACCACTTTTCTGCGGTATACTTCATCCACTCCCTGGTATATCAGGCCTTTCAGAGAGGTGTTATTTATGTGGCAGAGGACTGCTCAGAGCTACAGCTACCTAACGGAGGAAGAGATACGAGACTTGTGTACATCATGTGGTCTCACGAACTACTCGAGCAAAGTTCAGCAATCTTTCATCATGTTTTCTGCTCAGAAACCTTGA

Protein Analysis

344

Amino Acids

38.48

Weight (kDa)

9.23

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 168 - 284 7.2e-12 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 170 - 329 4.5e-08 Methyltransferase domain
Methyltransf_11 PF08241 176 - 276 6.1e-21 Methyltransferase domain
Methyltransf_25 PF13649 176 - 273 3e-17 Methyltransferase domain
Methyltransf_31 PF13847 176 - 280 3.1e-12 Methyltransferase domain
Methyltransf_12 PF08242 176 - 275 1.9e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 432
AccB7I CCANNNNNTGG 1 cut(s) 864
AccI GTMKAC 1 cut(s) 848
AccII CGCG 2 cut(s) 120, 509
AciI CCGC 6 cut(s) 97, 221, 507, 509, 516, 844
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 439, 563
AcyI GRCGYC 1 cut(s) 433
AfaI GTAC 2 cut(s) 359, 961
AfiI CCNNNNNNNGG 2 cut(s) 864, 937
AgsI TTSAA 4 cut(s) 280, 352, 365, 777
AhlI ACTAGT 1 cut(s) 449
AjnI CCWGG 2 cut(s) 252, 863
AluBI AGCT 6 cut(s) 156, 180, 377, 537, 921, 927
AluI AGCT 6 cut(s) 156, 180, 377, 537, 921, 927
Alw21I GWGCWC 1 cut(s) 164
Alw26I GTCTC 3 cut(s) 98, 945, 977
Ama87I CYCGRG 2 cut(s) 181, 985
AoxI GGCC 4 cut(s) 3, 235, 764, 875
ApeKI GCWGC 1 cut(s) 749
ApoI RAATTY 2 cut(s) 439, 563
AspLEI GCGC 3 cut(s) 122, 435, 601
AspS9I GGNCC 4 cut(s) 236, 328, 464, 765
AsuC2I CCSGG 1 cut(s) 462
AvaI CYCGRG 2 cut(s) 181, 985
AvaII GGWCC 2 cut(s) 328, 464
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 432
BanII GRGCYC 1 cut(s) 401
BauI CACGAG 1 cut(s) 798
Bbv12I GWGCWC 1 cut(s) 164
BbvI GCAGC 1 cut(s) 761
BccI CCATC 1 cut(s) 84
BceAI ACGGC 1 cut(s) 27
BciT130I CCWGG 2 cut(s) 254, 865
BcnI CCSGG 1 cut(s) 462
BcoDI GTCTC 3 cut(s) 98, 945, 977
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 4 cut(s) 378, 450, 524, 702
BfmI CTRYAG 1 cut(s) 922
BfoI RGCGCY 2 cut(s) 436, 602
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 1 cut(s) 750
BlsI GCNGC 1 cut(s) 751
Bme1390I CCNGG 3 cut(s) 254, 462, 865
Bme18I GGWCC 2 cut(s) 328, 464
BmeT110I CYCGRG 2 cut(s) 181, 985
BmgT120I GGNCC 4 cut(s) 236, 328, 464, 765
BmiI GGNNCC 5 cut(s) 15, 398, 434, 465, 832
BmrFI CCNGG 3 cut(s) 254, 462, 865
BmrI ACTGGG 1 cut(s) 200
BmsI GCATC 3 cut(s) 643, 721, 725
BmuI ACTGGG 1 cut(s) 200
BplI GAGNNNNNCTC 2 cut(s) 898, 930
BpmI CTGGAG 2 cut(s) 178, 609
Bpu10I CCTNAGC 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 462
BsaHI GRCGYC 1 cut(s) 433
BsaI GGTCTC 1 cut(s) 977
BsaJI CCNNGG 2 cut(s) 507, 863
BsaWI WCCGGW 1 cut(s) 145
BsaXI ACNNNNNCTCC 1 cut(s) 28
Bsc4I CCNNNNNNNGG 2 cut(s) 864, 937
Bse118I RCCGGY 1 cut(s) 743
Bse1I ACTGG 2 cut(s) 206, 247
BseBI CCWGG 2 cut(s) 254, 865
BseDI CCNNGG 2 cut(s) 507, 863
BseGI GGATG 2 cut(s) 535, 854
BseLI CCNNNNNNNGG 2 cut(s) 864, 937
BseMII CTCAG 3 cut(s) 375, 503, 929
BseNI ACTGG 2 cut(s) 206, 247
BseRI GAGGAG 3 cut(s) 10, 75, 78
BseXI GCAGC 1 cut(s) 761
BseYI CCCAGC 2 cut(s) 344, 373
BsgI GTGCAG 2 cut(s) 740, 768
Bsh1236I CGCG 2 cut(s) 120, 509
BshFI GGCC 4 cut(s) 5, 237, 766, 877
BshNI GGYRCC 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 164
BsiHKCI CYCGRG 2 cut(s) 181, 985
BsiSI CCGG 3 cut(s) 146, 461, 744
BslI CCNNNNNNNGG 2 cut(s) 864, 937
BsmAI GTCTC 3 cut(s) 98, 945, 977
BsmBI CGTCTC 1 cut(s) 98
BsnI GGCC 4 cut(s) 5, 237, 766, 877
Bso31I GGTCTC 1 cut(s) 977
BsoBI CYCGRG 2 cut(s) 181, 985
Bsp1286I GDGCHC 2 cut(s) 164, 401
Bsp1407I TGTACA 1 cut(s) 959
BspACI CCGC 6 cut(s) 97, 221, 507, 509, 516, 844
BspANI GGCC 4 cut(s) 5, 237, 766, 877
BspCNI CTCAG 3 cut(s) 376, 502, 928
BspFNI CGCG 2 cut(s) 120, 509
BspLI GGNNCC 5 cut(s) 15, 398, 434, 465, 832
BspQI GCTCTTC 1 cut(s) 146
BspT107I GGYRCC 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 977
BsrFI RCCGGY 1 cut(s) 743
BsrGI TGTACA 1 cut(s) 959
BsrI ACTGG 2 cut(s) 206, 247
BssAI RCCGGY 1 cut(s) 743
BssECI CCNNGG 2 cut(s) 507, 863
BssNAI GTATAC 1 cut(s) 849
BssNI GRCGYC 1 cut(s) 433
BssSI CACGAG 1 cut(s) 798
Bst1107I GTATAC 1 cut(s) 849
Bst2BI CACGAG 1 cut(s) 798
Bst2UI CCWGG 2 cut(s) 254, 865
Bst4CI ACNGT 3 cut(s) 337, 630, 761
Bst6I CTCTTC 3 cut(s) 146, 468, 936
BstACI GRCGYC 1 cut(s) 433
BstAPI GCANNNNNTGC 1 cut(s) 740
BstAUI TGTACA 1 cut(s) 959
BstDEI CTNAG 4 cut(s) 384, 489, 915, 1023
BstDSI CCRYGG 1 cut(s) 507
BstENI CCTNNNNNAGG 1 cut(s) 935
BstF5I GGATG 2 cut(s) 535, 854
BstFNI CGCG 2 cut(s) 120, 509
BstH2I RGCGCY 2 cut(s) 436, 602
BstHHI GCGC 3 cut(s) 122, 435, 601
BstMAI GTCTC 3 cut(s) 98, 945, 977
BstMWI GCNNNNNNNGC 6 cut(s) 11, 128, 210, 740, 749, 763
BstNI CCWGG 2 cut(s) 254, 865
BstSCI CCNGG 3 cut(s) 252, 460, 863
BstSFI CTRYAG 1 cut(s) 922
BstUI CGCG 2 cut(s) 120, 509
BstV1I GCAGC 1 cut(s) 761
BstZ17I GTATAC 1 cut(s) 849
BsuRI GGCC 4 cut(s) 5, 237, 766, 877
BtgI CCRYGG 1 cut(s) 507
BtsCI GGATG 2 cut(s) 535, 854
BtsI GCAGTG 1 cut(s) 752
BtsIMutI CAGTG 3 cut(s) 635, 752, 757
CfoI GCGC 3 cut(s) 122, 435, 601
Cfr10I RCCGGY 1 cut(s) 743
Cfr13I GGNCC 4 cut(s) 236, 328, 464, 765
Cfr42I CCGCGG 1 cut(s) 510
Csp6I GTAC 2 cut(s) 358, 960
CviAII CATG 4 cut(s) 201, 740, 966, 1012
CviQI GTAC 2 cut(s) 358, 960
DdeI CTNAG 4 cut(s) 384, 489, 915, 1023
DinI GGCGCC 1 cut(s) 434
DraI TTTAAA 2 cut(s) 481, 502
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 146, 468, 936
EarI CTCTTC 3 cut(s) 146, 468, 936
Eco147I AGGCCT 1 cut(s) 877
Eco24I GRGCYC 1 cut(s) 401
Eco31I GGTCTC 1 cut(s) 977
Eco47I GGWCC 2 cut(s) 328, 464
Eco88I CYCGRG 2 cut(s) 181, 985
EcoNI CCTNNNNNAGG 1 cut(s) 935
EcoO109I RGGNCCY 1 cut(s) 464
EcoRII CCWGG 2 cut(s) 252, 863
EcoT38I GRGCYC 1 cut(s) 401
EgeI GGCGCC 1 cut(s) 434
EheI GGCGCC 1 cut(s) 434
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 4 cut(s) 204, 743, 969, 1015
FatI CATG 4 cut(s) 200, 739, 965, 1011
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 1 cut(s) 750
FokI GGATG 2 cut(s) 542, 841
FriOI GRGCYC 1 cut(s) 401
Fsp4HI GCNGC 1 cut(s) 750
FspBI CTAG 4 cut(s) 378, 450, 524, 702
GlaI GCGC 3 cut(s) 121, 434, 600
GluI GCNGC 1 cut(s) 750
GsaI CCCAGC 2 cut(s) 348, 377
GsuI CTGGAG 2 cut(s) 178, 609
HaeII RGCGCY 2 cut(s) 436, 602
HaeIII GGCC 4 cut(s) 5, 237, 766, 877
HapII CCGG 3 cut(s) 146, 461, 744
HhaI GCGC 3 cut(s) 122, 435, 601
Hin1I GRCGYC 1 cut(s) 433
Hin1II CATG 4 cut(s) 204, 743, 969, 1015
Hin6I GCGC 3 cut(s) 120, 433, 599
HinP1I GCGC 3 cut(s) 120, 433, 599
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 1 cut(s) 673
HpaII CCGG 3 cut(s) 146, 461, 744
Hpy166II GTNNAC 3 cut(s) 370, 849, 960
Hpy188I TCNGA 5 cut(s) 613, 626, 884, 918, 1026
Hpy188III TCNNGA 4 cut(s) 467, 491, 588, 976
Hpy8I GTNNAC 3 cut(s) 370, 849, 960
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 506, 783
HpyCH4III ACNGT 3 cut(s) 337, 630, 761
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 6 cut(s) 131, 285, 716, 734, 749, 757
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 128, 210, 740, 749, 763
HpyF3I CTNAG 4 cut(s) 384, 489, 915, 1023
HpySE526I ACGT 1 cut(s) 360
Hsp92I GRCGYC 1 cut(s) 433
Hsp92II CATG 4 cut(s) 204, 743, 969, 1015
HspAI GCGC 3 cut(s) 120, 433, 599
KasI GGCGCC 1 cut(s) 432
KspI CCGCGG 1 cut(s) 510
LguI GCTCTTC 1 cut(s) 146
LmnI GCTCC 4 cut(s) 19, 159, 396, 627
Lsp1109I GCAGC 1 cut(s) 761
LweI GCATC 3 cut(s) 643, 721, 725
MaeI CTAG 4 cut(s) 378, 450, 524, 702
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 293, 337
MboII GAAGA 3 cut(s) 163, 485, 953
MhlI GDGCHC 2 cut(s) 164, 401
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 70, 439, 563
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 433
MmeI TCCRAC 2 cut(s) 649, 808
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 480, 501, 645
MslI CAYNNNNRTG 1 cut(s) 744
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 3 cut(s) 146, 461, 744
MspR9I CCNGG 3 cut(s) 254, 462, 865
MvaI CCWGG 2 cut(s) 254, 865
MvnI CGCG 2 cut(s) 120, 509
MwoI GCNNNNNNNGC 6 cut(s) 11, 128, 210, 740, 749, 763
NarI GGCGCC 1 cut(s) 433
NciI CCSGG 1 cut(s) 462
NlaIII CATG 4 cut(s) 204, 743, 969, 1015
NlaIV GGNNCC 5 cut(s) 15, 398, 434, 465, 832
NmuCI GTSAC 1 cut(s) 293
PaeR7I CTCGAG 1 cut(s) 985
PceI AGGCCT 1 cut(s) 877
PciSI GCTCTTC 1 cut(s) 146
PcsI WCGNNNNNNNCGW 2 cut(s) 805, 983
PfeI GAWTC 1 cut(s) 673
PflMI CCANNNNNTGG 1 cut(s) 864
PkrI GCNGC 1 cut(s) 751
PluTI GGCGCC 1 cut(s) 436
PpuMI RGGWCCY 1 cut(s) 464
Psp5II RGGWCCY 1 cut(s) 464
Psp6I CCWGG 2 cut(s) 252, 863
PspFI CCCAGC 2 cut(s) 344, 373
PspGI CCWGG 2 cut(s) 252, 863
PspN4I GGNNCC 5 cut(s) 15, 398, 434, 465, 832
PspPI GGNCC 4 cut(s) 236, 328, 464, 765
PspPPI RGGWCCY 1 cut(s) 464
PspXI VCTCGAGB 1 cut(s) 985
PsrI GAACNNNNNNTAC 2 cut(s) 210, 242
RsaI GTAC 2 cut(s) 359, 961
RsaNI GTAC 2 cut(s) 358, 960
RseI CAYNNNNRTG 1 cut(s) 744
SacII CCGCGG 1 cut(s) 510
SapI GCTCTTC 1 cut(s) 146
SaqAI TTAA 3 cut(s) 480, 501, 645
SatI GCNGC 1 cut(s) 750
Sau96I GGNCC 4 cut(s) 236, 328, 464, 765
ScrFI CCNGG 3 cut(s) 254, 462, 865
SduI GDGCHC 2 cut(s) 164, 401
SfaNI GCATC 3 cut(s) 643, 721, 725
SfcI CTRYAG 1 cut(s) 922
SfoI GGCGCC 1 cut(s) 434
Sfr274I CTCGAG 1 cut(s) 985
Sfr303I CCGCGG 1 cut(s) 510
SgrBI CCGCGG 1 cut(s) 510
SinI GGWCC 2 cut(s) 328, 464
SlaI CTCGAG 1 cut(s) 985
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 1 cut(s) 985
SmoI CTYRAG 1 cut(s) 985
SpeI ACTAGT 1 cut(s) 449
Sse9I AATT 3 cut(s) 70, 439, 563
SseBI AGGCCT 1 cut(s) 877
SsiI CCGC 6 cut(s) 97, 221, 507, 509, 516, 844
SspDI GGCGCC 1 cut(s) 432
SspMI CTAG 4 cut(s) 378, 450, 524, 702
StuI AGGCCT 1 cut(s) 877
StyD4I CCNGG 3 cut(s) 252, 460, 863
TaaI ACNGT 3 cut(s) 337, 630, 761
TaiI ACGT 1 cut(s) 363
TaqI TCGA 3 cut(s) 557, 729, 986
TasI AATT 3 cut(s) 70, 439, 563
TatI WGTACW 1 cut(s) 959
TfiI GAWTC 1 cut(s) 673
Tru1I TTAA 3 cut(s) 480, 501, 645
Tru9I TTAA 3 cut(s) 480, 501, 645
TscAI CASTG 3 cut(s) 635, 759, 764
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 1 cut(s) 749
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 5 cut(s) 231, 435, 486, 843, 997
TspGWI ACGGA 1 cut(s) 950
TspRI CASTG 3 cut(s) 635, 759, 764
Van91I CCANNNNNTGG 1 cut(s) 864
VpaK11BI GGWCC 2 cut(s) 328, 464
XagI CCTNNNNNAGG 1 cut(s) 935
XapI RAATTY 2 cut(s) 439, 563
XhoI CTCGAG 1 cut(s) 985
XmiI GTMKAC 1 cut(s) 848
XspI CTAG 4 cut(s) 378, 450, 524, 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.