Rh2BG023900

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002821
Physical Location & Seq
Forward (+)
1608869 .. 1611455
2587 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG023900.1

Sequence Viewer

Length: 903 bp
ATGGCGATGGCTCCCTCATCTGTCAATAAGCAGAGCTCAAGGACTGATGAGAGTCAAAGCTCGGAGATTGAGTTGTTATCCTGCCCAGTATGCTATGAACCACTAATACGAAAAGGCCCACCTGGTCTAATCCTGCAAGCAATATGCAGGTCATCGTTCGACTGCAAGAAATGCTACAAGTCGTATTCCAGTGAACACAACTACTTGGATCTCACTGTTACTGCTGGATTGAAGAAGTACGTTGAAGTTAAACCAGTTGGCACTGAGTTATTCAGGAGATCCTTCATGTCCTTCATTTATGAAAAAGGTTATCGTCAGTATTTTAGACGATATGGCTTCCCAGGTCCTGATGAAGAATTTAAAATGGCTCAGGACTACTTTAAATCCACTGAAGGTGGTGTTCTAGTGGATGTTAGCTGTGGGAGTGGTTTGTTCGCGATAAGATTCGCCAAATCTGGGACCTATTCAGGAGTTGTGGCACTTGATTTTTCTGAAAATATGCTTCTCCAGTGCTATGATTATCTTTTGAGAGACCCTACTAATTTTTTGGCTACTGATCTTGTTCTTGTGAGGGCCGATGTTTCTAGGCTTCCATTCACATCATGTTCAGTTGATGCTATCCATGCCGGCGCTGCATTGCACTGCTGGCCATCTCCTTCCAACGCTGTTGCTGAAATCACTCGTGTATTACGAAGTGGTGGCATCTTTGTTGGGACCACTTTTCTGCGGTCTAGTCCTTCAAGTACTTCCTGGATATTCAAACCACTCAATGAGAGGGTTCTTCCCAGCGGGAATTATTTTAGGGAGGAAGAGATTGAGGACTTGTGTACATCATGCGGTCTCACAAACTACTCGAGCAAAGTTGACCAATCTTTCATCATCTTTTCTGCTCAGAAACCTTGA

Protein Analysis

300

Amino Acids

33.47

Weight (kDa)

6.81

Isoelectric Point (pI)

65.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 126 - 262 1.4e-10 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 130 - 285 8e-08 Methyltransferase domain
Methyltransf_31 PF13847 131 - 242 9.6e-11 Methyltransferase domain
Methyltransf_11 PF08241 136 - 237 1.1e-17 Methyltransferase domain
Methyltransf_25 PF13649 136 - 234 5.2e-14 Methyltransferase domain
Methyltransf_12 PF08242 136 - 236 9.3e-10 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 138
AccII CGCG 1 cut(s) 437
AciI CCGC 3 cut(s) 727, 789, 837
AclWI GGATC 2 cut(s) 216, 273
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 1 cut(s) 356
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 3 cut(s) 239, 745, 829
AfiI CCNNNNNNNGG 1 cut(s) 456
AgsI TTSAA 4 cut(s) 232, 245, 741, 760
AjnI CCWGG 3 cut(s) 121, 340, 749
AluBI AGCT 3 cut(s) 36, 60, 417
AluI AGCT 3 cut(s) 36, 60, 417
Alw21I GWGCWC 1 cut(s) 38
Alw26I GTCTC 2 cut(s) 525, 845
AlwI GGATC 2 cut(s) 216, 273
AlwNI CAGNNNCTG 1 cut(s) 347
Ama87I CYCGRG 1 cut(s) 853
AoxI GGCC 3 cut(s) 115, 573, 647
ApeKI GCWGC 1 cut(s) 632
ApoI RAATTY 1 cut(s) 356
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 5 cut(s) 116, 344, 459, 573, 714
AvaI CYCGRG 1 cut(s) 853
AvaII GGWCC 3 cut(s) 344, 459, 714
BalI TGGCCA 1 cut(s) 649
BanII GRGCYC 1 cut(s) 38
BauI CACGAG 1 cut(s) 681
Bbv12I GWGCWC 1 cut(s) 38
BbvI GCAGC 1 cut(s) 619
BccI CCATC 1 cut(s) 658
BciT130I CCWGG 3 cut(s) 123, 342, 751
BcoDI GTCTC 2 cut(s) 525, 845
BfaI CTAG 3 cut(s) 404, 585, 732
BfoI RGCGCY 1 cut(s) 633
BfuAI ACCTGC 1 cut(s) 138
BisI GCNGC 1 cut(s) 633
BlsI GCNGC 1 cut(s) 634
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 3 cut(s) 123, 342, 751
Bme18I GGWCC 3 cut(s) 344, 459, 714
BmeT110I CYCGRG 1 cut(s) 853
BmgT120I GGNCC 5 cut(s) 116, 344, 459, 573, 714
BmiI GGNNCC 3 cut(s) 12, 460, 715
BmrFI CCNGG 3 cut(s) 123, 342, 751
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 2 cut(s) 604, 711
BmuI ACTGGG 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 491
Bpu10I CCTNAGC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 22
BsaI GGTCTC 2 cut(s) 525, 845
BsaJI CCNNGG 1 cut(s) 340
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 1 cut(s) 456
Bse118I RCCGGY 1 cut(s) 626
Bse1I ACTGG 4 cut(s) 86, 189, 254, 508
Bse3DI GCAATG 1 cut(s) 635
BseBI CCWGG 3 cut(s) 123, 342, 751
BseDI CCNNGG 1 cut(s) 340
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 1 cut(s) 456
BseMI GCAATG 1 cut(s) 635
BseMII CTCAG 2 cut(s) 255, 383
BseNI ACTGG 4 cut(s) 86, 189, 254, 508
BseXI GCAGC 1 cut(s) 619
BseYI CCCAGC 1 cut(s) 785
Bsh1236I CGCG 1 cut(s) 437
BshFI GGCC 3 cut(s) 117, 575, 649
BsiHKAI GWGCWC 1 cut(s) 38
BsiHKCI CYCGRG 1 cut(s) 853
BsiSI CCGG 1 cut(s) 627
BslFI GGGAC 2 cut(s) 472, 727
BslI CCNNNNNNNGG 1 cut(s) 456
BsmAI GTCTC 2 cut(s) 525, 845
BsmFI GGGAC 2 cut(s) 472, 727
BsnI GGCC 3 cut(s) 117, 575, 649
Bso31I GGTCTC 2 cut(s) 525, 845
BsoBI CYCGRG 1 cut(s) 853
Bsp1286I GDGCHC 1 cut(s) 38
Bsp1407I TGTACA 1 cut(s) 827
Bsp143I GATC 3 cut(s) 208, 278, 556
Bsp68I TCGCGA 1 cut(s) 437
BspACI CCGC 3 cut(s) 727, 789, 837
BspANI GGCC 3 cut(s) 117, 575, 649
BspCNI CTCAG 2 cut(s) 256, 382
BspFNI CGCG 1 cut(s) 437
BspLI GGNNCC 3 cut(s) 12, 460, 715
BspMI ACCTGC 1 cut(s) 138
BspPI GGATC 2 cut(s) 216, 273
BspTNI GGTCTC 2 cut(s) 525, 845
BsrDI GCAATG 1 cut(s) 635
BsrFI RCCGGY 1 cut(s) 626
BsrGI TGTACA 1 cut(s) 827
BsrI ACTGG 4 cut(s) 86, 189, 254, 508
BssAI RCCGGY 1 cut(s) 626
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 3 cut(s) 208, 278, 556
BssSI CACGAG 1 cut(s) 681
Bst2BI CACGAG 1 cut(s) 681
Bst2UI CCWGG 3 cut(s) 123, 342, 751
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 804
BstAPI GCANNNNNTGC 1 cut(s) 171
BstAUI TGTACA 1 cut(s) 827
BstC8I GCNNGC 3 cut(s) 138, 628, 647
BstDEI CTNAG 3 cut(s) 264, 369, 891
BstF5I GGATG 1 cut(s) 415
BstFNI CGCG 1 cut(s) 437
BstH2I RGCGCY 1 cut(s) 633
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 3 cut(s) 211, 281, 559
BstMAI GTCTC 2 cut(s) 525, 845
BstMBI GATC 3 cut(s) 208, 278, 556
BstMWI GCNNNNNNNGC 5 cut(s) 90, 171, 623, 632, 646
BstNI CCWGG 3 cut(s) 123, 342, 751
BstSCI CCNGG 3 cut(s) 121, 340, 749
BstUI CGCG 1 cut(s) 437
BstV1I GCAGC 1 cut(s) 619
BstX2I RGATCY 2 cut(s) 208, 278
BstYI RGATCY 2 cut(s) 208, 278
BsuRI GGCC 3 cut(s) 117, 575, 649
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 415
BtsI GCAGTG 1 cut(s) 640
BtsIMutI CAGTG 6 cut(s) 196, 213, 261, 387, 515, 640
BtuMI TCGCGA 1 cut(s) 437
BveI ACCTGC 1 cut(s) 138
Cac8I GCNNGC 3 cut(s) 138, 628, 647
CaiI CAGNNNCTG 1 cut(s) 347
CfoI GCGC 1 cut(s) 632
Cfr10I RCCGGY 1 cut(s) 626
Cfr13I GGNCC 5 cut(s) 116, 344, 459, 573, 714
CsiI ACCWGGT 1 cut(s) 121
Csp6I GTAC 3 cut(s) 238, 744, 828
CviAII CATG 4 cut(s) 286, 603, 623, 834
CviQI GTAC 3 cut(s) 238, 744, 828
DdeI CTNAG 3 cut(s) 264, 369, 891
DpnI GATC 3 cut(s) 210, 280, 558
DpnII GATC 3 cut(s) 208, 278, 556
DraI TTTAAA 2 cut(s) 361, 382
EaeI YGGCCR 1 cut(s) 647
Eam1104I CTCTTC 1 cut(s) 804
EarI CTCTTC 1 cut(s) 804
Ecl136II GAGCTC 1 cut(s) 36
Eco24I GRGCYC 1 cut(s) 38
Eco31I GGTCTC 2 cut(s) 525, 845
Eco47I GGWCC 3 cut(s) 344, 459, 714
Eco53kI GAGCTC 1 cut(s) 36
Eco57I CTGAAG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 853
EcoICRI GAGCTC 1 cut(s) 36
EcoO109I RGGNCCY 2 cut(s) 344, 459
EcoRII CCWGG 3 cut(s) 121, 340, 749
EcoT38I GRGCYC 1 cut(s) 38
FaeI CATG 4 cut(s) 289, 606, 626, 837
FaqI GGGAC 2 cut(s) 472, 727
FatI CATG 4 cut(s) 285, 602, 622, 833
FauI CCCGC 1 cut(s) 782
Fnu4HI GCNGC 1 cut(s) 633
FokI GGATG 1 cut(s) 422
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 1 cut(s) 633
FspBI CTAG 3 cut(s) 404, 585, 732
GlaI GCGC 1 cut(s) 631
GluI GCNGC 1 cut(s) 633
GsaI CCCAGC 1 cut(s) 789
GsuI CTGGAG 1 cut(s) 491
HaeII RGCGCY 1 cut(s) 633
HaeIII GGCC 3 cut(s) 117, 575, 649
HapII CCGG 1 cut(s) 627
HhaI GCGC 1 cut(s) 632
Hin1II CATG 4 cut(s) 289, 606, 626, 837
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 1 cut(s) 865
HindII GTYRAC 1 cut(s) 865
HinfI GANTC 2 cut(s) 52, 444
HpaII CCGG 1 cut(s) 627
Hpy166II GTNNAC 3 cut(s) 194, 828, 865
Hpy188I TCNGA 3 cut(s) 64, 493, 894
Hpy188III TCNNGA 5 cut(s) 274, 347, 371, 436, 468
Hpy8I GTNNAC 3 cut(s) 194, 828, 865
HpyAV CCTTC 5 cut(s) 292, 301, 386, 666, 747
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 5 cut(s) 136, 147, 165, 635, 640
HpyF10VI GCNNNNNNNGC 5 cut(s) 90, 171, 623, 632, 646
HpyF3I CTNAG 3 cut(s) 264, 369, 891
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 4 cut(s) 289, 606, 626, 837
HspAI GCGC 1 cut(s) 630
KroI GCCGGC 1 cut(s) 626
KroNI GCCGGC 1 cut(s) 628
Kzo9I GATC 3 cut(s) 208, 278, 556
LmnI GCTCC 1 cut(s) 16
Lsp1109I GCAGC 1 cut(s) 619
LweI GCATC 2 cut(s) 604, 711
MabI ACCWGGT 1 cut(s) 121
MaeI CTAG 3 cut(s) 404, 585, 732
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 1 cut(s) 217
MalI GATC 3 cut(s) 210, 280, 558
MboI GATC 3 cut(s) 208, 278, 556
MboII GAAGA 4 cut(s) 244, 365, 773, 821
MflI RGATCY 2 cut(s) 208, 278
MhlI GDGCHC 1 cut(s) 38
MlsI TGGCCA 1 cut(s) 649
MluCI AATT 3 cut(s) 356, 541, 793
MluNI TGGCCA 1 cut(s) 649
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 684
MnlI CCTC 5 cut(s) 25, 564, 768, 799, 811
Mox20I TGGCCA 1 cut(s) 649
MroNI GCCGGC 1 cut(s) 626
MscI TGGCCA 1 cut(s) 649
MseI TTAA 3 cut(s) 249, 360, 381
Msp20I TGGCCA 1 cut(s) 649
MspA1I CMGCKG 1 cut(s) 789
MspI CCGG 1 cut(s) 627
MspR9I CCNGG 3 cut(s) 123, 342, 751
MvaI CCWGG 3 cut(s) 123, 342, 751
MvnI CGCG 1 cut(s) 437
MwoI GCNNNNNNNGC 5 cut(s) 90, 171, 623, 632, 646
NaeI GCCGGC 1 cut(s) 628
NdeII GATC 3 cut(s) 208, 278, 556
NgoMIV GCCGGC 1 cut(s) 626
NlaIII CATG 4 cut(s) 289, 606, 626, 837
NlaIV GGNNCC 3 cut(s) 12, 460, 715
NruI TCGCGA 1 cut(s) 437
PaeR7I CTCGAG 1 cut(s) 853
PcsI WCGNNNNNNNCGW 1 cut(s) 688
PdiI GCCGGC 1 cut(s) 628
PfeI GAWTC 1 cut(s) 444
PfoI TCCNGGA 1 cut(s) 749
PkrI GCNGC 1 cut(s) 634
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
PpuMI RGGWCCY 2 cut(s) 344, 459
Psp124BI GAGCTC 1 cut(s) 38
Psp5II RGGWCCY 2 cut(s) 344, 459
Psp6I CCWGG 3 cut(s) 121, 340, 749
PspFI CCCAGC 1 cut(s) 785
PspGI CCWGG 3 cut(s) 121, 340, 749
PspN4I GGNNCC 3 cut(s) 12, 460, 715
PspPI GGNCC 5 cut(s) 116, 344, 459, 573, 714
PspPPI RGGWCCY 2 cut(s) 344, 459
PspXI VCTCGAGB 1 cut(s) 853
PsrI GAACNNNNNNTAC 2 cut(s) 90, 122
PstNI CAGNNNCTG 1 cut(s) 347
PsuI RGATCY 2 cut(s) 208, 278
RruI TCGCGA 1 cut(s) 437
RsaI GTAC 3 cut(s) 239, 745, 829
RsaNI GTAC 3 cut(s) 238, 744, 828
SacI GAGCTC 1 cut(s) 38
SaqAI TTAA 3 cut(s) 249, 360, 381
SatI GCNGC 1 cut(s) 633
Sau3AI GATC 3 cut(s) 208, 278, 556
Sau96I GGNCC 5 cut(s) 116, 344, 459, 573, 714
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 3 cut(s) 123, 342, 751
SduI GDGCHC 1 cut(s) 38
SexAI ACCWGGT 1 cut(s) 121
SfaNI GCATC 2 cut(s) 604, 711
Sfr274I CTCGAG 1 cut(s) 853
SinI GGWCC 3 cut(s) 344, 459, 714
SlaI CTCGAG 1 cut(s) 853
SmlI CTYRAG 2 cut(s) 37, 853
SmoI CTYRAG 2 cut(s) 37, 853
Sse9I AATT 3 cut(s) 356, 541, 793
SsiI CCGC 3 cut(s) 727, 789, 837
SspMI CTAG 3 cut(s) 404, 585, 732
SstI GAGCTC 1 cut(s) 38
StyD4I CCNGG 3 cut(s) 121, 340, 749
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 1 cut(s) 243
TaqI TCGA 2 cut(s) 159, 854
TasI AATT 3 cut(s) 356, 541, 793
TatI WGTACW 2 cut(s) 743, 827
TfiI GAWTC 1 cut(s) 444
Tru1I TTAA 3 cut(s) 249, 360, 381
Tru9I TTAA 3 cut(s) 249, 360, 381
TscAI CASTG 6 cut(s) 196, 220, 268, 394, 515, 647
TseI GCWGC 1 cut(s) 632
TspDTI ATGAA 6 cut(s) 111, 274, 283, 315, 366, 865
TspRI CASTG 6 cut(s) 196, 220, 268, 394, 515, 647
VpaK11BI GGWCC 3 cut(s) 344, 459, 714
XapI RAATTY 1 cut(s) 356
XhoI CTCGAG 1 cut(s) 853
XspI CTAG 3 cut(s) 404, 585, 732
ZrmI AGTACT 1 cut(s) 745
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.