Rroxscaffold_2G00154050

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002821
Physical Location & Seq
Reverse (-)
90747051 .. 90749538
2488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00154050.1

Sequence Viewer

Length: 1059 bp
ATGGGAACGGCTCTCTCATCTTTCAATGAATCGAAGCAGAGCTCAACGACGGAGGAGAGTCAAAGCTCGGAGATTGAGTTGTTATCCTGCCCGGTATGCTATGAACCACTAACACGAAAAGGCCCACCTGGTCTAATCCTGCAAGCAATATGCAGGTCATCGTTCGACTGCAAGAAATGCTACAAGTCGTATTCCAGTAAACACAGCTACTTGGATCTCACTGTTACTGCTGGATTGAAGAAGTACGTTGAAGTTAAACCAGTTGGCACTGAGTTATTCAGGAGATCCTTCATGTCCTTCATCTATGAAAGAGGCTATCGTCAGGGTTTTAAACGATATGGCTTCCCAGGTCCTGATGAAGAGTTTAAAATGGCTCAGGACTACTTTAAATCCGCTGACGGTGGTGTTCTAGTGGATGTTAGCTGTGGGAGTGGACGAGTGTGCTATGATTATCTTAAGAGAGACCCTATGACTCTTTTGGCTACGCAAGCAACATGCAGGTCATCGTTCGAATGCAAGAAATGCTACAAGTTGTATTCCAGTAAACACAACTACTTGGATCTCACTGTTACTGCTGGATTGAAGAAGTACGTTGAAGTTAAACCAGTTGACACTGAGATATTCAGGAGATCCTTCATGTCCTTCATCTATAAAAGAGGTGGTATTCTAGTGGATGTTAGCTGTGGGAGTGGTTTGTTCGCAATAAGATTTGCCAAATCTGGGACCTACTATGGAGTTGTGGCACTTGATTTTTCGGAAAATATGCTTCTCCAGTGCTATGATTATCTTAAGAGAGACCCTATGACTCTCAATAATGATCTCGTTCTTGTGAGGGCTGATGTTTCTAGGCTTGTATTCATCACATCAAGTTCGATTGATGCTGTCCATGCCGGCGCCGCGTTGCACTGCTGGTCCTCTCCTTCCAATGCTGTTGCTGAAATCACTCTTGTATTACGTAGTGGTGGCATCTTTGTTGGAACCACTTTTCTGCAGTCTAGTCCTTCAGCTCCCTGGATATTCAAACCACTCACAGAGATAACATGCATTCTCATATTCTAG

Protein Analysis

352

Amino Acids

39.31

Weight (kDa)

8.72

Isoelectric Point (pI)

58.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 217 - 328 1.3e-07 ubiE/COQ5 methyltransferase family
Methyltransf_31 PF13847 222 - 328 1.2e-06 Methyltransferase domain
Methyltransf_11 PF08241 224 - 325 2.8e-14 Methyltransferase domain
Methyltransf_25 PF13649 224 - 322 2e-11 Methyltransferase domain
Methyltransf_12 PF08242 224 - 324 3.8e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 144, 489
AccB1I GGYRCC 1 cut(s) 893
AccII CGCG 1 cut(s) 899
AciI CCGC 2 cut(s) 393, 897
AclWI GGATC 4 cut(s) 222, 279, 567, 624
AcuI CTGAAG 1 cut(s) 987
AcyI GRCGYC 1 cut(s) 894
AfaI GTAC 2 cut(s) 245, 590
AfiI CCNNNNNNNGG 1 cut(s) 720
AflII CTTAAG 2 cut(s) 455, 788
AgsI TTSAA 6 cut(s) 25, 238, 251, 583, 596, 1021
AjnI CCWGG 3 cut(s) 127, 346, 1010
AluBI AGCT 6 cut(s) 42, 66, 207, 423, 681, 1007
AluI AGCT 6 cut(s) 42, 66, 207, 423, 681, 1007
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 2 cut(s) 456, 789
AlwI GGATC 4 cut(s) 222, 279, 567, 624
AlwNI CAGNNNCTG 1 cut(s) 353
AoxI GGCC 1 cut(s) 121
AspLEI GCGC 1 cut(s) 896
AspS9I GGNCC 4 cut(s) 122, 350, 723, 912
AsuC2I CCSGG 1 cut(s) 92
AsuII TTCGAA 1 cut(s) 510
AvaII GGWCC 3 cut(s) 350, 723, 912
BanI GGYRCC 1 cut(s) 893
BanII GRGCYC 1 cut(s) 44
Bbv12I GWGCWC 1 cut(s) 44
BceAI ACGGC 1 cut(s) 24
BciT130I CCWGG 3 cut(s) 129, 348, 1012
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 2 cut(s) 456, 789
BfaI CTAG 5 cut(s) 410, 668, 846, 996, 1057
BfmI CTRYAG 1 cut(s) 989
BfoI RGCGCY 1 cut(s) 897
BfrI CTTAAG 2 cut(s) 455, 788
BfuAI ACCTGC 2 cut(s) 144, 489
BisI GCNGC 1 cut(s) 897
BlsI GCNGC 1 cut(s) 898
Bme1390I CCNGG 4 cut(s) 92, 129, 348, 1012
Bme18I GGWCC 3 cut(s) 350, 723, 912
BmgT120I GGNCC 4 cut(s) 122, 350, 723, 912
BmiI GGNNCC 3 cut(s) 724, 895, 979
BmrFI CCNGG 4 cut(s) 92, 129, 348, 1012
BmsI GCATC 2 cut(s) 868, 975
BpmI CTGGAG 1 cut(s) 755
Bpu10I CCTNAGC 1 cut(s) 375
Bpu14I TTCGAA 1 cut(s) 510
BpuMI CCSGG 1 cut(s) 92
BsaAI YACGTR 1 cut(s) 956
BsaHI GRCGYC 1 cut(s) 894
BsaI GGTCTC 2 cut(s) 456, 789
BsaJI CCNNGG 2 cut(s) 346, 1010
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 720
Bse118I RCCGGY 1 cut(s) 890
Bse1I ACTGG 5 cut(s) 195, 260, 540, 605, 772
BseBI CCWGG 3 cut(s) 129, 348, 1012
BseDI CCNNGG 2 cut(s) 346, 1010
BseGI GGATG 2 cut(s) 421, 679
BseLI CCNNNNNNNGG 1 cut(s) 720
BseMII CTCAG 3 cut(s) 261, 389, 606
BseNI ACTGG 5 cut(s) 195, 260, 540, 605, 772
BseRI GAGGAG 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 899
BshFI GGCC 1 cut(s) 123
BshNI GGYRCC 1 cut(s) 893
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 2 cut(s) 92, 891
BslFI GGGAC 1 cut(s) 736
BslI CCNNNNNNNGG 1 cut(s) 720
BsmAI GTCTC 2 cut(s) 456, 789
BsmFI GGGAC 1 cut(s) 736
BsmI GAATGC 2 cut(s) 518, 1044
BsnI GGCC 1 cut(s) 123
Bso31I GGTCTC 2 cut(s) 456, 789
Bsp119I TTCGAA 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 5 cut(s) 214, 284, 559, 629, 817
BspACI CCGC 2 cut(s) 393, 897
BspANI GGCC 1 cut(s) 123
BspCNI CTCAG 3 cut(s) 262, 388, 607
BspFNI CGCG 1 cut(s) 899
BspLI GGNNCC 3 cut(s) 724, 895, 979
BspMAI CTGCAG 1 cut(s) 993
BspMI ACCTGC 2 cut(s) 144, 489
BspPI GGATC 4 cut(s) 222, 279, 567, 624
BspT104I TTCGAA 1 cut(s) 510
BspT107I GGYRCC 1 cut(s) 893
BspTI CTTAAG 2 cut(s) 455, 788
BspTNI GGTCTC 2 cut(s) 456, 789
BsrFI RCCGGY 1 cut(s) 890
BsrI ACTGG 5 cut(s) 195, 260, 540, 605, 772
BssAI RCCGGY 1 cut(s) 890
BssECI CCNNGG 2 cut(s) 346, 1010
BssMI GATC 5 cut(s) 214, 284, 559, 629, 817
BssNI GRCGYC 1 cut(s) 894
Bst2UI CCWGG 3 cut(s) 129, 348, 1012
Bst4CI ACNGT 3 cut(s) 223, 401, 568
Bst6I CTCTTC 1 cut(s) 354
BstACI GRCGYC 1 cut(s) 894
BstAFI CTTAAG 2 cut(s) 455, 788
BstAPI GCANNNNNTGC 2 cut(s) 177, 522
BstBAI YACGTR 1 cut(s) 956
BstBI TTCGAA 1 cut(s) 510
BstC8I GCNNGC 3 cut(s) 144, 489, 892
BstDEI CTNAG 3 cut(s) 270, 375, 615
BstF5I GGATG 2 cut(s) 421, 679
BstFNI CGCG 1 cut(s) 899
BstH2I RGCGCY 1 cut(s) 897
BstHHI GCGC 1 cut(s) 896
BstKTI GATC 5 cut(s) 217, 287, 562, 632, 820
BstMAI GTCTC 2 cut(s) 456, 789
BstMBI GATC 5 cut(s) 214, 284, 559, 629, 817
BstMWI GCNNNNNNNGC 6 cut(s) 96, 177, 488, 522, 887, 896
BstNI CCWGG 3 cut(s) 129, 348, 1012
BstNSI RCATGY 2 cut(s) 498, 1044
BstSCI CCNGG 4 cut(s) 90, 127, 346, 1010
BstSFI CTRYAG 1 cut(s) 989
BstSNI TACGTA 1 cut(s) 956
BstUI CGCG 1 cut(s) 899
BstX2I RGATCY 4 cut(s) 214, 284, 559, 629
BstYI RGATCY 4 cut(s) 214, 284, 559, 629
BsuRI GGCC 1 cut(s) 123
BtsCI GGATG 2 cut(s) 421, 679
BtsI GCAGTG 1 cut(s) 904
BtsIMutI CAGTG 6 cut(s) 219, 267, 564, 612, 779, 904
BveI ACCTGC 2 cut(s) 144, 489
Cac8I GCNNGC 3 cut(s) 144, 489, 892
CaiI CAGNNNCTG 1 cut(s) 353
CfoI GCGC 1 cut(s) 896
Cfr10I RCCGGY 1 cut(s) 890
Cfr13I GGNCC 4 cut(s) 122, 350, 723, 912
CsiI ACCWGGT 1 cut(s) 127
Csp6I GTAC 2 cut(s) 244, 589
CviAII CATG 5 cut(s) 292, 495, 637, 887, 1041
CviQI GTAC 2 cut(s) 244, 589
DdeI CTNAG 3 cut(s) 270, 375, 615
DinI GGCGCC 1 cut(s) 895
DpnI GATC 5 cut(s) 216, 286, 561, 631, 819
DpnII GATC 5 cut(s) 214, 284, 559, 629, 817
DraI TTTAAA 3 cut(s) 331, 367, 388
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Ecl136II GAGCTC 1 cut(s) 42
Eco105I TACGTA 1 cut(s) 956
Eco24I GRGCYC 1 cut(s) 44
Eco31I GGTCTC 2 cut(s) 456, 789
Eco47I GGWCC 3 cut(s) 350, 723, 912
Eco53kI GAGCTC 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 987
EcoICRI GAGCTC 1 cut(s) 42
EcoO109I RGGNCCY 2 cut(s) 350, 723
EcoRII CCWGG 3 cut(s) 127, 346, 1010
EcoT22I ATGCAT 1 cut(s) 1046
EcoT38I GRGCYC 1 cut(s) 44
EgeI GGCGCC 1 cut(s) 895
EheI GGCGCC 1 cut(s) 895
FaeI CATG 5 cut(s) 295, 498, 640, 890, 1044
FaqI GGGAC 1 cut(s) 736
FatI CATG 5 cut(s) 291, 494, 636, 886, 1040
Fnu4HI GCNGC 1 cut(s) 897
FokI GGATG 2 cut(s) 428, 686
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 1 cut(s) 897
FspBI CTAG 5 cut(s) 410, 668, 846, 996, 1057
GlaI GCGC 1 cut(s) 895
GluI GCNGC 1 cut(s) 897
GsuI CTGGAG 1 cut(s) 755
HaeII RGCGCY 1 cut(s) 897
HaeIII GGCC 1 cut(s) 123
HapII CCGG 2 cut(s) 92, 891
HhaI GCGC 1 cut(s) 896
Hin1I GRCGYC 1 cut(s) 894
Hin1II CATG 5 cut(s) 295, 498, 640, 890, 1044
Hin6I GCGC 1 cut(s) 894
HinP1I GCGC 1 cut(s) 894
HincII GTYRAC 1 cut(s) 610
HindII GTYRAC 1 cut(s) 610
HinfI GANTC 4 cut(s) 29, 58, 472, 805
HpaII CCGG 2 cut(s) 92, 891
Hpy166II GTNNAC 4 cut(s) 200, 434, 545, 610
Hpy188I TCNGA 2 cut(s) 70, 757
Hpy188III TCNNGA 4 cut(s) 280, 353, 377, 625
Hpy8I GTNNAC 4 cut(s) 200, 434, 545, 610
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 6 cut(s) 298, 307, 643, 652, 930, 1011
HpyCH4III ACNGT 3 cut(s) 223, 401, 568
HpyCH4IV ACGT 3 cut(s) 246, 591, 955
HpyCH4V TGCA 8 cut(s) 142, 153, 171, 498, 516, 904, 991, 1044
HpyF10VI GCNNNNNNNGC 6 cut(s) 96, 177, 488, 522, 887, 896
HpyF3I CTNAG 3 cut(s) 270, 375, 615
HpySE526I ACGT 3 cut(s) 246, 591, 955
Hsp92I GRCGYC 1 cut(s) 894
Hsp92II CATG 5 cut(s) 295, 498, 640, 890, 1044
HspAI GCGC 1 cut(s) 894
KasI GGCGCC 1 cut(s) 893
KroI GCCGGC 1 cut(s) 890
KroNI GCCGGC 1 cut(s) 892
Kzo9I GATC 5 cut(s) 214, 284, 559, 629, 817
LmnI GCTCC 1 cut(s) 1012
LweI GCATC 2 cut(s) 868, 975
MabI ACCWGGT 1 cut(s) 127
MaeI CTAG 5 cut(s) 410, 668, 846, 996, 1057
MaeII ACGT 3 cut(s) 246, 591, 955
MaeIII GTNAC 2 cut(s) 223, 568
MalI GATC 5 cut(s) 216, 286, 561, 631, 819
MboI GATC 5 cut(s) 214, 284, 559, 629, 817
MboII GAAGA 3 cut(s) 250, 371, 595
MflI RGATCY 4 cut(s) 214, 284, 559, 629
MhlI GDGCHC 1 cut(s) 44
Mly113I GGCGCC 1 cut(s) 894
MlyI GAGTC 3 cut(s) 67, 466, 799
MmeI TCCRAC 1 cut(s) 955
MnlI CCTC 5 cut(s) 46, 305, 650, 825, 925
Mph1103I ATGCAT 1 cut(s) 1046
MroNI GCCGGC 1 cut(s) 890
MseI TTAA 7 cut(s) 255, 330, 366, 387, 456, 600, 789
MspA1I CMGCKG 1 cut(s) 395
MspCI CTTAAG 2 cut(s) 455, 788
MspI CCGG 2 cut(s) 92, 891
MspR9I CCNGG 4 cut(s) 92, 129, 348, 1012
Mva1269I GAATGC 2 cut(s) 518, 1044
MvaI CCWGG 3 cut(s) 129, 348, 1012
MvnI CGCG 1 cut(s) 899
MwoI GCNNNNNNNGC 6 cut(s) 96, 177, 488, 522, 887, 896
NaeI GCCGGC 1 cut(s) 892
NarI GGCGCC 1 cut(s) 894
NciI CCSGG 1 cut(s) 92
NdeII GATC 5 cut(s) 214, 284, 559, 629, 817
NgoMIV GCCGGC 1 cut(s) 890
NlaIII CATG 5 cut(s) 295, 498, 640, 890, 1044
NlaIV GGNNCC 3 cut(s) 724, 895, 979
NsiI ATGCAT 1 cut(s) 1046
NspI RCATGY 2 cut(s) 498, 1044
NspV TTCGAA 1 cut(s) 510
PctI GAATGC 2 cut(s) 518, 1044
PdiI GCCGGC 1 cut(s) 892
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 1 cut(s) 898
PleI GAGTC 3 cut(s) 66, 466, 799
PluTI GGCGCC 1 cut(s) 897
PpsI GAGTC 3 cut(s) 66, 466, 799
Ppu21I YACGTR 1 cut(s) 956
PpuMI RGGWCCY 2 cut(s) 350, 723
Psp124BI GAGCTC 1 cut(s) 44
Psp5II RGGWCCY 2 cut(s) 350, 723
Psp6I CCWGG 3 cut(s) 127, 346, 1010
PspGI CCWGG 3 cut(s) 127, 346, 1010
PspN4I GGNNCC 3 cut(s) 724, 895, 979
PspPI GGNCC 4 cut(s) 122, 350, 723, 912
PspPPI RGGWCCY 2 cut(s) 350, 723
PstI CTGCAG 1 cut(s) 993
PstNI CAGNNNCTG 1 cut(s) 353
PsuI RGATCY 4 cut(s) 214, 284, 559, 629
RsaI GTAC 2 cut(s) 245, 590
RsaNI GTAC 2 cut(s) 244, 589
SacI GAGCTC 1 cut(s) 44
SaqAI TTAA 7 cut(s) 255, 330, 366, 387, 456, 600, 789
SatI GCNGC 1 cut(s) 897
Sau3AI GATC 5 cut(s) 214, 284, 559, 629, 817
Sau96I GGNCC 4 cut(s) 122, 350, 723, 912
SchI GAGTC 3 cut(s) 67, 466, 799
ScrFI CCNGG 4 cut(s) 92, 129, 348, 1012
SduI GDGCHC 1 cut(s) 44
SexAI ACCWGGT 1 cut(s) 127
SfaNI GCATC 2 cut(s) 868, 975
SfcI CTRYAG 1 cut(s) 989
SfoI GGCGCC 1 cut(s) 895
SfuI TTCGAA 1 cut(s) 510
SinI GGWCC 3 cut(s) 350, 723, 912
SmlI CTYRAG 2 cut(s) 455, 788
SmoI CTYRAG 2 cut(s) 455, 788
SnaBI TACGTA 1 cut(s) 956
SsiI CCGC 2 cut(s) 393, 897
SspDI GGCGCC 1 cut(s) 893
SspMI CTAG 5 cut(s) 410, 668, 846, 996, 1057
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 4 cut(s) 90, 127, 346, 1010
TaaI ACNGT 3 cut(s) 223, 401, 568
TaiI ACGT 3 cut(s) 249, 594, 958
TaqI TCGA 4 cut(s) 32, 165, 510, 872
TauI GCSGC 1 cut(s) 899
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 7 cut(s) 255, 330, 366, 387, 456, 600, 789
Tru9I TTAA 7 cut(s) 255, 330, 366, 387, 456, 600, 789
TscAI CASTG 6 cut(s) 226, 274, 571, 619, 779, 911
TspDTI ATGAA 9 cut(s) 42, 117, 280, 289, 321, 372, 625, 634, 847
TspGWI ACGGA 1 cut(s) 65
TspRI CASTG 6 cut(s) 226, 274, 571, 619, 779, 911
Vha464I CTTAAG 2 cut(s) 455, 788
VpaK11BI GGWCC 3 cut(s) 350, 723, 912
XceI RCATGY 2 cut(s) 498, 1044
XspI CTAG 5 cut(s) 410, 668, 846, 996, 1057
Zsp2I ATGCAT 1 cut(s) 1046
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.