Rh2DG024000

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002821
Physical Location & Seq
Forward (+)
1414238 .. 1417849
3612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG024000.1

Sequence Viewer

Length: 990 bp
ATGCCAAAAAACAGAAAGAAAGCAAGAAAGAAAAAAGAGAGAAAAAAAATAGACACTGCAGGAGCAATAGCAAATCAATCAAACTTCATGGCGATGGCTCCCTCATCTGTCAATAAGCAGAGCTCAAGGACTGATGAGAGTCAAAGCTCGGAGATTGAGTTGTTATCCTGCCCAGTATGCTATGAACCACTAATACGAAAAGGCCCACCTGGTCTAATCCTGCAAGCAATATGCAGGTCATCGTTCGACTGCAAGAAATGCTACAAGTCGTATTCCAGTGAACACAACTACTTGGATCTCACTGTTACTGCTGGATTGAAGAAGTACGTTGAAGTTAAACCAGTTGGCACTGAGTTATTCAGGAGATCCTTCATGTCCTTCATTTATGAAAAAGGTTATCGTCAGTATTTTAGACGATATGGCTTCCCAGGTCCTGATGAAGAATTTGAAATGGCTCAGGACTACTTTAAATCCACTGAAGGTGGTGTTCTAGTGGATGTTAGCTGTGGGAGTGGTTTGTTCGCGATAAGATTCGCCAAATCTGGGACCTATTCTGGAGTTGTGGCACTTGATTTTTCTGAAAATATGCTTCTCCAGTGCTATGATTATCTTATGAGAGACCCTACTAATTTTTTGGCTACTGATCTTGTTCTTGTGAGGGCCGATGTTTCTAGGCTTCCATTCACATCATGTTCAGTTGATGCTGTCCATGCCGGCGCTGCATTGCACTGCTGGCCATCTCCTTCCAACGCTGTTGCTGAAATCACTCGTGTATTACGAAGTGGTGGCATCTTTGTTGGGACCACTTTTCTGCGGTCTAGTCCTTCAAGTACTTCCTGGATATTCAAACCACTCAATGAGAGGGTTCTTCCCAGCGGAAATTATTTTAGGGAGGAAGAGATTGAGGACTTGTGTACATCATGCGGTCTCACAAACTACTCGAGCAAAGTTGACCAATCTTTCATCATCTTTTCTGCTCAGAAACCTTGA

Protein Analysis

329

Amino Acids

36.81

Weight (kDa)

8.76

Isoelectric Point (pI)

59.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 156 - 291 4.9e-11 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 159 - 314 2.6e-07 Methyltransferase domain
Methyltransf_31 PF13847 160 - 271 1.3e-10 Methyltransferase domain
Methyltransf_11 PF08241 165 - 266 5e-18 Methyltransferase domain
Methyltransf_25 PF13649 165 - 263 2.8e-14 Methyltransferase domain
Methyltransf_12 PF08242 165 - 265 1.2e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 225
AccII CGCG 1 cut(s) 524
AciI CCGC 3 cut(s) 814, 876, 924
AclWI GGATC 2 cut(s) 303, 360
AcoI YGGCCR 1 cut(s) 734
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 3 cut(s) 326, 832, 916
AfiI CCNNNNNNNGG 1 cut(s) 543
AgsI TTSAA 5 cut(s) 319, 332, 449, 828, 847
AjnI CCWGG 3 cut(s) 208, 427, 836
AluBI AGCT 3 cut(s) 123, 147, 504
AluI AGCT 3 cut(s) 123, 147, 504
Alw21I GWGCWC 1 cut(s) 125
Alw26I GTCTC 2 cut(s) 612, 932
AlwI GGATC 2 cut(s) 303, 360
AlwNI CAGNNNCTG 1 cut(s) 434
Ama87I CYCGRG 1 cut(s) 940
AoxI GGCC 3 cut(s) 202, 660, 734
ApeKI GCWGC 1 cut(s) 719
ApoI RAATTY 1 cut(s) 443
AspLEI GCGC 1 cut(s) 719
AspS9I GGNCC 5 cut(s) 203, 431, 546, 660, 801
AvaI CYCGRG 1 cut(s) 940
AvaII GGWCC 3 cut(s) 431, 546, 801
BalI TGGCCA 1 cut(s) 736
BanII GRGCYC 1 cut(s) 125
BauI CACGAG 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 125
BbvI GCAGC 1 cut(s) 706
BccI CCATC 2 cut(s) 88, 745
BciT130I CCWGG 3 cut(s) 210, 429, 838
BcoDI GTCTC 2 cut(s) 612, 932
BfaI CTAG 3 cut(s) 491, 672, 819
BfmI CTRYAG 1 cut(s) 57
BfoI RGCGCY 1 cut(s) 720
BfuAI ACCTGC 1 cut(s) 225
BisI GCNGC 1 cut(s) 720
BlsI GCNGC 1 cut(s) 721
BmcAI AGTACT 1 cut(s) 832
Bme1390I CCNGG 3 cut(s) 210, 429, 838
Bme18I GGWCC 3 cut(s) 431, 546, 801
BmeT110I CYCGRG 1 cut(s) 940
BmgT120I GGNCC 5 cut(s) 203, 431, 546, 660, 801
BmiI GGNNCC 3 cut(s) 99, 547, 802
BmrFI CCNGG 3 cut(s) 210, 429, 838
BmrI ACTGGG 1 cut(s) 167
BmsI GCATC 2 cut(s) 691, 798
BmuI ACTGGG 1 cut(s) 167
BpmI CTGGAG 2 cut(s) 576, 578
Bpu10I CCTNAGC 1 cut(s) 456
BpuEI CTTGAG 1 cut(s) 109
BsaI GGTCTC 2 cut(s) 612, 932
BsaJI CCNNGG 1 cut(s) 427
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 543
Bse118I RCCGGY 1 cut(s) 713
Bse1I ACTGG 4 cut(s) 173, 276, 341, 595
Bse3DI GCAATG 1 cut(s) 722
BseBI CCWGG 3 cut(s) 210, 429, 838
BseDI CCNNGG 1 cut(s) 427
BseGI GGATG 1 cut(s) 502
BseLI CCNNNNNNNGG 1 cut(s) 543
BseMI GCAATG 1 cut(s) 722
BseMII CTCAG 2 cut(s) 342, 470
BseNI ACTGG 4 cut(s) 173, 276, 341, 595
BseXI GCAGC 1 cut(s) 706
BseYI CCCAGC 1 cut(s) 872
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 3 cut(s) 204, 662, 736
BsiHKAI GWGCWC 1 cut(s) 125
BsiHKCI CYCGRG 1 cut(s) 940
BsiSI CCGG 1 cut(s) 714
BslFI GGGAC 2 cut(s) 559, 814
BslI CCNNNNNNNGG 1 cut(s) 543
BsmAI GTCTC 2 cut(s) 612, 932
BsmFI GGGAC 2 cut(s) 559, 814
BsnI GGCC 3 cut(s) 204, 662, 736
Bso31I GGTCTC 2 cut(s) 612, 932
BsoBI CYCGRG 1 cut(s) 940
Bsp1286I GDGCHC 1 cut(s) 125
Bsp1407I TGTACA 1 cut(s) 914
Bsp143I GATC 3 cut(s) 295, 365, 643
Bsp68I TCGCGA 1 cut(s) 524
BspACI CCGC 3 cut(s) 814, 876, 924
BspANI GGCC 3 cut(s) 204, 662, 736
BspCNI CTCAG 2 cut(s) 343, 469
BspFNI CGCG 1 cut(s) 524
BspLI GGNNCC 3 cut(s) 99, 547, 802
BspMAI CTGCAG 1 cut(s) 61
BspMI ACCTGC 1 cut(s) 225
BspPI GGATC 2 cut(s) 303, 360
BspTNI GGTCTC 2 cut(s) 612, 932
BsrDI GCAATG 1 cut(s) 722
BsrFI RCCGGY 1 cut(s) 713
BsrGI TGTACA 1 cut(s) 914
BsrI ACTGG 4 cut(s) 173, 276, 341, 595
BssAI RCCGGY 1 cut(s) 713
BssECI CCNNGG 1 cut(s) 427
BssMI GATC 3 cut(s) 295, 365, 643
BssSI CACGAG 1 cut(s) 768
Bst2BI CACGAG 1 cut(s) 768
Bst2UI CCWGG 3 cut(s) 210, 429, 838
Bst4CI ACNGT 1 cut(s) 304
Bst6I CTCTTC 1 cut(s) 891
BstAPI GCANNNNNTGC 1 cut(s) 258
BstAUI TGTACA 1 cut(s) 914
BstC8I GCNNGC 3 cut(s) 225, 715, 734
BstDEI CTNAG 3 cut(s) 351, 456, 978
BstF5I GGATG 1 cut(s) 502
BstFNI CGCG 1 cut(s) 524
BstH2I RGCGCY 1 cut(s) 720
BstHHI GCGC 1 cut(s) 719
BstKTI GATC 3 cut(s) 298, 368, 646
BstMAI GTCTC 2 cut(s) 612, 932
BstMBI GATC 3 cut(s) 295, 365, 643
BstMWI GCNNNNNNNGC 5 cut(s) 177, 258, 710, 719, 733
BstNI CCWGG 3 cut(s) 210, 429, 838
BstSCI CCNGG 3 cut(s) 208, 427, 836
BstSFI CTRYAG 1 cut(s) 57
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 1 cut(s) 706
BstX2I RGATCY 2 cut(s) 295, 365
BstYI RGATCY 2 cut(s) 295, 365
BsuRI GGCC 3 cut(s) 204, 662, 736
BtgZI GCGATG 1 cut(s) 107
BtsCI GGATG 1 cut(s) 502
BtsI GCAGTG 2 cut(s) 54, 727
BtsIMutI CAGTG 7 cut(s) 54, 283, 300, 348, 474, 602, 727
BtuMI TCGCGA 1 cut(s) 524
BveI ACCTGC 1 cut(s) 225
Cac8I GCNNGC 3 cut(s) 225, 715, 734
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 1 cut(s) 719
Cfr10I RCCGGY 1 cut(s) 713
Cfr13I GGNCC 5 cut(s) 203, 431, 546, 660, 801
CsiI ACCWGGT 1 cut(s) 208
Csp6I GTAC 3 cut(s) 325, 831, 915
CviAII CATG 5 cut(s) 88, 373, 690, 710, 921
CviQI GTAC 3 cut(s) 325, 831, 915
DdeI CTNAG 3 cut(s) 351, 456, 978
DpnI GATC 3 cut(s) 297, 367, 645
DpnII GATC 3 cut(s) 295, 365, 643
DraI TTTAAA 1 cut(s) 469
EaeI YGGCCR 1 cut(s) 734
Eam1104I CTCTTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 891
Ecl136II GAGCTC 1 cut(s) 123
Eco24I GRGCYC 1 cut(s) 125
Eco31I GGTCTC 2 cut(s) 612, 932
Eco47I GGWCC 3 cut(s) 431, 546, 801
Eco53kI GAGCTC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 498
Eco88I CYCGRG 1 cut(s) 940
EcoICRI GAGCTC 1 cut(s) 123
EcoO109I RGGNCCY 2 cut(s) 431, 546
EcoRII CCWGG 3 cut(s) 208, 427, 836
EcoT38I GRGCYC 1 cut(s) 125
FaeI CATG 5 cut(s) 91, 376, 693, 713, 924
FaqI GGGAC 2 cut(s) 559, 814
FatI CATG 5 cut(s) 87, 372, 689, 709, 920
Fnu4HI GCNGC 1 cut(s) 720
FokI GGATG 1 cut(s) 509
FriOI GRGCYC 1 cut(s) 125
Fsp4HI GCNGC 1 cut(s) 720
FspBI CTAG 3 cut(s) 491, 672, 819
GlaI GCGC 1 cut(s) 718
GluI GCNGC 1 cut(s) 720
GsaI CCCAGC 1 cut(s) 876
GsuI CTGGAG 2 cut(s) 576, 578
HaeII RGCGCY 1 cut(s) 720
HaeIII GGCC 3 cut(s) 204, 662, 736
HapII CCGG 1 cut(s) 714
HhaI GCGC 1 cut(s) 719
Hin1II CATG 5 cut(s) 91, 376, 693, 713, 924
Hin6I GCGC 1 cut(s) 717
HinP1I GCGC 1 cut(s) 717
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 2 cut(s) 139, 531
HpaII CCGG 1 cut(s) 714
Hpy166II GTNNAC 3 cut(s) 281, 915, 952
Hpy188I TCNGA 3 cut(s) 151, 580, 981
Hpy188III TCNNGA 5 cut(s) 361, 434, 458, 523, 555
Hpy8I GTNNAC 3 cut(s) 281, 915, 952
HpyAV CCTTC 5 cut(s) 379, 388, 473, 753, 834
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 327
HpyCH4V TGCA 6 cut(s) 59, 223, 234, 252, 722, 727
HpyF10VI GCNNNNNNNGC 5 cut(s) 177, 258, 710, 719, 733
HpyF3I CTNAG 3 cut(s) 351, 456, 978
HpySE526I ACGT 1 cut(s) 327
Hsp92II CATG 5 cut(s) 91, 376, 693, 713, 924
HspAI GCGC 1 cut(s) 717
KroI GCCGGC 1 cut(s) 713
KroNI GCCGGC 1 cut(s) 715
Kzo9I GATC 3 cut(s) 295, 365, 643
LmnI GCTCC 2 cut(s) 62, 103
Lsp1109I GCAGC 1 cut(s) 706
LweI GCATC 2 cut(s) 691, 798
MabI ACCWGGT 1 cut(s) 208
MaeI CTAG 3 cut(s) 491, 672, 819
MaeII ACGT 1 cut(s) 327
MaeIII GTNAC 1 cut(s) 304
MalI GATC 3 cut(s) 297, 367, 645
MboI GATC 3 cut(s) 295, 365, 643
MboII GAAGA 4 cut(s) 331, 452, 860, 908
MflI RGATCY 2 cut(s) 295, 365
MhlI GDGCHC 1 cut(s) 125
MlsI TGGCCA 1 cut(s) 736
MluCI AATT 3 cut(s) 443, 628, 880
MluNI TGGCCA 1 cut(s) 736
MlyI GAGTC 1 cut(s) 148
MmeI TCCRAC 1 cut(s) 771
MnlI CCTC 5 cut(s) 112, 651, 855, 886, 898
Mox20I TGGCCA 1 cut(s) 736
MroNI GCCGGC 1 cut(s) 713
MscI TGGCCA 1 cut(s) 736
MseI TTAA 2 cut(s) 336, 468
MslI CAYNNNNRTG 1 cut(s) 92
Msp20I TGGCCA 1 cut(s) 736
MspA1I CMGCKG 1 cut(s) 876
MspI CCGG 1 cut(s) 714
MspR9I CCNGG 3 cut(s) 210, 429, 838
MvaI CCWGG 3 cut(s) 210, 429, 838
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 5 cut(s) 177, 258, 710, 719, 733
NaeI GCCGGC 1 cut(s) 715
NdeII GATC 3 cut(s) 295, 365, 643
NgoMIV GCCGGC 1 cut(s) 713
NlaIII CATG 5 cut(s) 91, 376, 693, 713, 924
NlaIV GGNNCC 3 cut(s) 99, 547, 802
NruI TCGCGA 1 cut(s) 524
PaeR7I CTCGAG 1 cut(s) 940
PcsI WCGNNNNNNNCGW 1 cut(s) 775
PdiI GCCGGC 1 cut(s) 715
PfeI GAWTC 1 cut(s) 531
PfoI TCCNGGA 1 cut(s) 836
PkrI GCNGC 1 cut(s) 721
PleI GAGTC 1 cut(s) 147
PpsI GAGTC 1 cut(s) 147
PpuMI RGGWCCY 2 cut(s) 431, 546
Psp124BI GAGCTC 1 cut(s) 125
Psp5II RGGWCCY 2 cut(s) 431, 546
Psp6I CCWGG 3 cut(s) 208, 427, 836
PspFI CCCAGC 1 cut(s) 872
PspGI CCWGG 3 cut(s) 208, 427, 836
PspN4I GGNNCC 3 cut(s) 99, 547, 802
PspPI GGNCC 5 cut(s) 203, 431, 546, 660, 801
PspPPI RGGWCCY 2 cut(s) 431, 546
PspXI VCTCGAGB 1 cut(s) 940
PsrI GAACNNNNNNTAC 2 cut(s) 177, 209
PstI CTGCAG 1 cut(s) 61
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 2 cut(s) 295, 365
RruI TCGCGA 1 cut(s) 524
RsaI GTAC 3 cut(s) 326, 832, 916
RsaNI GTAC 3 cut(s) 325, 831, 915
RseI CAYNNNNRTG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 125
SaqAI TTAA 2 cut(s) 336, 468
SatI GCNGC 1 cut(s) 720
Sau3AI GATC 3 cut(s) 295, 365, 643
Sau96I GGNCC 5 cut(s) 203, 431, 546, 660, 801
ScaI AGTACT 1 cut(s) 832
SchI GAGTC 1 cut(s) 148
ScrFI CCNGG 3 cut(s) 210, 429, 838
SduI GDGCHC 1 cut(s) 125
SexAI ACCWGGT 1 cut(s) 208
SfaNI GCATC 2 cut(s) 691, 798
SfcI CTRYAG 1 cut(s) 57
Sfr274I CTCGAG 1 cut(s) 940
SinI GGWCC 3 cut(s) 431, 546, 801
SlaI CTCGAG 1 cut(s) 940
SmiMI CAYNNNNRTG 1 cut(s) 92
SmlI CTYRAG 2 cut(s) 124, 940
SmoI CTYRAG 2 cut(s) 124, 940
Sse9I AATT 3 cut(s) 443, 628, 880
SsiI CCGC 3 cut(s) 814, 876, 924
SspMI CTAG 3 cut(s) 491, 672, 819
SstI GAGCTC 1 cut(s) 125
StyD4I CCNGG 3 cut(s) 208, 427, 836
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 1 cut(s) 330
TaqI TCGA 2 cut(s) 246, 941
TasI AATT 3 cut(s) 443, 628, 880
TatI WGTACW 2 cut(s) 830, 914
TfiI GAWTC 1 cut(s) 531
Tru1I TTAA 2 cut(s) 336, 468
Tru9I TTAA 2 cut(s) 336, 468
TscAI CASTG 7 cut(s) 61, 283, 307, 355, 481, 602, 734
TseI GCWGC 1 cut(s) 719
TspDTI ATGAA 7 cut(s) 76, 198, 361, 370, 402, 453, 952
TspRI CASTG 7 cut(s) 61, 283, 307, 355, 481, 602, 734
VpaK11BI GGWCC 3 cut(s) 431, 546, 801
XapI RAATTY 1 cut(s) 443
XhoI CTCGAG 1 cut(s) 940
XspI CTAG 3 cut(s) 491, 672, 819
ZrmI AGTACT 1 cut(s) 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.