RLG00000015811

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002821
Physical Location & Seq
Forward (+)
1576892 .. 1579287
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015811

Sequence Viewer

Length: 942 bp
ATGGCCACTGAGAAAACCGAGAGCTTGGAAATGGAGCTGTTATCATGCCCGGTATGCTATGAACCGCTAATTCGAAAAGGCCCAACTGGTCTCAACTTGCAAGCAATATACAGGTCGGTTTTCGAATGCAAGAAATGCCACAAGTCTTATTCAAGTAAAGATATTTACTTGGATCTTACTGTTACTGCTGGCTTGAAGAAATACGCTGAAGTCAAACCAGCAGGGACTGAGTTCTTCAGGAGACCATTTGTTTCCTTCATTTACGAAAGAGGGTGGCGTCAGTATTTCGGAGGCGGCTTCCCAGGTCCTGATGAAGAGTTTAAAATGGCTCAGGAATACTTTAAACCTGTCAAAGGTGGTGTTCTAGTGGATGTGAGCTGTGGGAGTGGTATGTTTGCGAGGAAATTTGCCAAATCCGGAACCTATTCTGGAGTTGTTACACTTGATTTTTCCGAAAATATGCTCCGGCAGTGCTATGGTTTCTTGACAAGAGACCCTACTACTCTTTTAGCTGCGAATCTTGCTCTTGTAAGGGCAGATGTTTCTAGGCTTCCATTTGCATCAGGTTCGGTTGATGCTGTACATGCCGGCGCAGCATTGCACTGCTGGCCCTCTCCTTCCAATGCTGTTGCTGAAATCACTCGTGTATTAAGAAGTGGTGGAATCTTTGTTGGAACCACTTTTCTGCGGTCTAGTCCTTCCACTCCATGGATATTCAAGCCCTTTAGAGAAGTAAATACTTATTCTCATTCATTGACAACCAAAATACAACACCCAACTACTTTGATTACTGAATATGCCATAGCAGCACAGAGGATCCATCAAACCAATACCTATTTGACGGAGGAAGAGCTTGAGGACTTATGTACATCGTTCGGTCTCACCAATTACTCGATCAAAGTTCAGCAATCTTTCATCATGTTTTCTGCTCAGAAACCATGA

Protein Analysis

314

Amino Acids

34.92

Weight (kDa)

8.68

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 116 - 228 5.2e-11 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 116 - 298 1.4e-09 Methyltransferase domain
Methyltransf_31 PF13847 118 - 229 6.2e-12 Methyltransferase domain
Methyltransf_11 PF08241 123 - 224 3e-20 Methyltransferase domain
Methyltransf_25 PF13649 123 - 221 7.9e-16 Methyltransferase domain
Methyltransf_12 PF08242 123 - 223 1.3e-09 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 708
AccIII TCCGGA 1 cut(s) 416
AciI CCGC 3 cut(s) 65, 294, 688
AclWI GGATC 3 cut(s) 180, 811, 824
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 404
AcuI CTGAAG 2 cut(s) 220, 228
AcyI GRCGYC 1 cut(s) 277
AfaI GTAC 2 cut(s) 582, 868
AfiI CCNNNNNNNGG 2 cut(s) 353, 708
AgsI TTSAA 3 cut(s) 153, 196, 718
AjnI CCWGG 1 cut(s) 301
AluBI AGCT 5 cut(s) 24, 37, 378, 512, 853
AluI AGCT 5 cut(s) 24, 37, 378, 512, 853
Alw26I GTCTC 4 cut(s) 95, 235, 486, 884
AlwI GGATC 3 cut(s) 180, 811, 824
AlwNI CAGNNNCTG 2 cut(s) 227, 308
Aor13HI TCCGGA 1 cut(s) 416
AoxI GGCC 3 cut(s) 3, 79, 608
ApeKI GCWGC 3 cut(s) 512, 593, 806
ApoI RAATTY 1 cut(s) 404
Asp700I GAANNNNTTC 1 cut(s) 424
AspLEI GCGC 1 cut(s) 593
AspS9I GGNCC 3 cut(s) 80, 305, 609
AsuC2I CCSGG 1 cut(s) 50
AsuHPI GGTGA 1 cut(s) 874
AsuII TTCGAA 2 cut(s) 73, 123
AvaII GGWCC 1 cut(s) 305
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 816
BauI CACGAG 1 cut(s) 642
BbvI GCAGC 3 cut(s) 499, 605, 818
BccI CCATC 1 cut(s) 828
BcgI CGANNNNNNTGC 2 cut(s) 549, 583
BciT130I CCWGG 1 cut(s) 303
BcnI CCSGG 1 cut(s) 50
BcoDI GTCTC 4 cut(s) 95, 235, 486, 884
BfaI CTAG 3 cut(s) 365, 546, 693
BisI GCNGC 4 cut(s) 295, 513, 594, 807
BlsI GCNGC 4 cut(s) 296, 514, 595, 808
Bme1390I CCNGG 2 cut(s) 50, 303
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 3 cut(s) 80, 305, 609
BmiI GGNNCC 3 cut(s) 421, 676, 818
BmrFI CCNGG 2 cut(s) 50, 303
BmsI GCATC 2 cut(s) 565, 569
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 1 cut(s) 330
Bpu14I TTCGAA 2 cut(s) 73, 123
BpuEI CTTGAG 1 cut(s) 875
BpuMI CCSGG 1 cut(s) 50
BsaHI GRCGYC 1 cut(s) 277
BsaI GGTCTC 4 cut(s) 95, 235, 486, 884
BsaJI CCNNGG 2 cut(s) 301, 707
BsaWI WCCGGW 1 cut(s) 416
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 708
Bse118I RCCGGY 1 cut(s) 587
Bse1I ACTGG 1 cut(s) 91
Bse3DI GCAATG 1 cut(s) 596
BseAI TCCGGA 1 cut(s) 416
BseBI CCWGG 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 301, 707
BseGI GGATG 1 cut(s) 376
BseLI CCNNNNNNNGG 2 cut(s) 353, 708
BseMI GCAATG 1 cut(s) 596
BseMII CTCAG 2 cut(s) 219, 344
BseNI ACTGG 1 cut(s) 91
BseXI GCAGC 3 cut(s) 499, 605, 818
BshFI GGCC 3 cut(s) 5, 81, 610
BsiSI CCGG 4 cut(s) 50, 417, 466, 588
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 2 cut(s) 353, 708
BsmAI GTCTC 4 cut(s) 95, 235, 486, 884
BsmFI GGGAC 1 cut(s) 238
BsmI GAATGC 1 cut(s) 131
BsnI GGCC 3 cut(s) 5, 81, 610
Bso31I GGTCTC 4 cut(s) 95, 235, 486, 884
Bsp119I TTCGAA 2 cut(s) 73, 123
Bsp13I TCCGGA 1 cut(s) 416
Bsp1407I TGTACA 2 cut(s) 580, 866
Bsp143I GATC 3 cut(s) 172, 816, 894
Bsp19I CCATGG 1 cut(s) 707
BspACI CCGC 3 cut(s) 65, 294, 688
BspANI GGCC 3 cut(s) 5, 81, 610
BspCNI CTCAG 2 cut(s) 220, 343
BspEI TCCGGA 1 cut(s) 416
BspLI GGNNCC 3 cut(s) 421, 676, 818
BspPI GGATC 3 cut(s) 180, 811, 824
BspQI GCTCTTC 1 cut(s) 843
BspT104I TTCGAA 2 cut(s) 73, 123
BspTNI GGTCTC 4 cut(s) 95, 235, 486, 884
BsrDI GCAATG 1 cut(s) 596
BsrFI RCCGGY 1 cut(s) 587
BsrGI TGTACA 2 cut(s) 580, 866
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 587
BssECI CCNNGG 2 cut(s) 301, 707
BssMI GATC 3 cut(s) 172, 816, 894
BssNI GRCGYC 1 cut(s) 277
BssSI CACGAG 1 cut(s) 642
BssT1I CCWWGG 1 cut(s) 707
Bst2BI CACGAG 1 cut(s) 642
Bst2UI CCWGG 1 cut(s) 303
Bst4CI ACNGT 1 cut(s) 181
Bst6I CTCTTC 2 cut(s) 309, 843
BstACI GRCGYC 1 cut(s) 277
BstAPI GCANNNNNTGC 1 cut(s) 135
BstAUI TGTACA 2 cut(s) 580, 866
BstBI TTCGAA 2 cut(s) 73, 123
BstC8I GCNNGC 4 cut(s) 102, 190, 589, 608
BstDEI CTNAG 4 cut(s) 9, 228, 330, 930
BstDSI CCRYGG 1 cut(s) 707
BstENI CCTNNNNNAGG 1 cut(s) 351
BstF5I GGATG 1 cut(s) 376
BstHHI GCGC 1 cut(s) 593
BstKTI GATC 3 cut(s) 175, 819, 897
BstMAI GTCTC 4 cut(s) 95, 235, 486, 884
BstMBI GATC 3 cut(s) 172, 816, 894
BstMWI GCNNNNNNNGC 7 cut(s) 54, 135, 521, 584, 593, 607, 806
BstNI CCWGG 1 cut(s) 303
BstNSI RCATGY 1 cut(s) 587
BstSCI CCNGG 2 cut(s) 48, 301
BstV1I GCAGC 3 cut(s) 499, 605, 818
BstX2I RGATCY 2 cut(s) 172, 816
BstYI RGATCY 2 cut(s) 172, 816
BsuRI GGCC 3 cut(s) 5, 81, 610
BtgI CCRYGG 1 cut(s) 707
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 2 cut(s) 476, 601
BtsIMutI CAGTG 3 cut(s) 6, 476, 601
Cac8I GCNNGC 4 cut(s) 102, 190, 589, 608
CaiI CAGNNNCTG 2 cut(s) 227, 308
CfoI GCGC 1 cut(s) 593
Cfr10I RCCGGY 1 cut(s) 587
Cfr13I GGNCC 3 cut(s) 80, 305, 609
CseI GACGC 1 cut(s) 266
Csp6I GTAC 2 cut(s) 581, 867
CviAII CATG 5 cut(s) 45, 584, 708, 919, 939
CviQI GTAC 2 cut(s) 581, 867
DdeI CTNAG 4 cut(s) 9, 228, 330, 930
DpnI GATC 3 cut(s) 174, 818, 896
DpnII GATC 3 cut(s) 172, 816, 894
DraI TTTAAA 2 cut(s) 322, 343
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 309, 843
EarI CTCTTC 2 cut(s) 309, 843
Eco130I CCWWGG 1 cut(s) 707
Eco31I GGTCTC 4 cut(s) 95, 235, 486, 884
Eco47I GGWCC 1 cut(s) 305
Eco57I CTGAAG 2 cut(s) 220, 228
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 305
EcoRII CCWGG 1 cut(s) 301
EcoT14I CCWWGG 1 cut(s) 707
ErhI CCWWGG 1 cut(s) 707
FaeI CATG 5 cut(s) 48, 587, 711, 922, 942
FaqI GGGAC 1 cut(s) 238
FatI CATG 5 cut(s) 44, 583, 707, 918, 938
Fnu4HI GCNGC 4 cut(s) 295, 513, 594, 807
FokI GGATG 1 cut(s) 383
Fsp4HI GCNGC 4 cut(s) 295, 513, 594, 807
FspBI CTAG 3 cut(s) 365, 546, 693
GlaI GCGC 1 cut(s) 592
GluI GCNGC 4 cut(s) 295, 513, 594, 807
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 3 cut(s) 5, 81, 610
HapII CCGG 4 cut(s) 50, 417, 466, 588
HgaI GACGC 1 cut(s) 266
HhaI GCGC 1 cut(s) 593
Hin1I GRCGYC 1 cut(s) 277
Hin1II CATG 5 cut(s) 48, 587, 711, 922, 942
Hin6I GCGC 1 cut(s) 591
HinP1I GCGC 1 cut(s) 591
HinfI GANTC 2 cut(s) 517, 663
HpaII CCGG 4 cut(s) 50, 417, 466, 588
HphI GGTGA 1 cut(s) 874
Hpy188I TCNGA 3 cut(s) 290, 454, 933
Hpy188III TCNNGA 6 cut(s) 238, 308, 332, 417, 429, 484
HpyAV CCTTC 3 cut(s) 265, 627, 708
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 4 cut(s) 100, 129, 560, 601
HpyF10VI GCNNNNNNNGC 7 cut(s) 54, 135, 521, 584, 593, 607, 806
HpyF3I CTNAG 4 cut(s) 9, 228, 330, 930
Hsp92I GRCGYC 1 cut(s) 277
Hsp92II CATG 5 cut(s) 48, 587, 711, 922, 942
HspAI GCGC 1 cut(s) 591
Kpn2I TCCGGA 1 cut(s) 416
KroI GCCGGC 1 cut(s) 587
KroNI GCCGGC 1 cut(s) 589
Kzo9I GATC 3 cut(s) 172, 816, 894
LguI GCTCTTC 1 cut(s) 843
LmnI GCTCC 2 cut(s) 34, 468
Lsp1109I GCAGC 3 cut(s) 499, 605, 818
LweI GCATC 2 cut(s) 565, 569
MaeI CTAG 3 cut(s) 365, 546, 693
MaeIII GTNAC 2 cut(s) 181, 436
MalI GATC 3 cut(s) 174, 818, 896
MboI GATC 3 cut(s) 172, 816, 894
MboII GAAGA 4 cut(s) 208, 226, 326, 860
MflI RGATCY 2 cut(s) 172, 816
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 69, 404, 886
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 652
MnlI CCTC 7 cut(s) 263, 284, 393, 622, 807, 838, 850
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 416
MroNI GCCGGC 1 cut(s) 587
MroXI GAANNNNTTC 1 cut(s) 424
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 321, 342, 650
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 50, 417, 466, 588
MspR9I CCNGG 2 cut(s) 50, 303
Mva1269I GAATGC 1 cut(s) 131
MvaI CCWGG 1 cut(s) 303
MwoI GCNNNNNNNGC 7 cut(s) 54, 135, 521, 584, 593, 607, 806
NaeI GCCGGC 1 cut(s) 589
NciI CCSGG 1 cut(s) 50
NcoI CCATGG 1 cut(s) 707
NdeII GATC 3 cut(s) 172, 816, 894
NgoMIV GCCGGC 1 cut(s) 587
NlaIII CATG 5 cut(s) 48, 587, 711, 922, 942
NlaIV GGNNCC 3 cut(s) 421, 676, 818
NspI RCATGY 1 cut(s) 587
NspV TTCGAA 2 cut(s) 73, 123
PciSI GCTCTTC 1 cut(s) 843
PctI GAATGC 1 cut(s) 131
PdiI GCCGGC 1 cut(s) 589
PdmI GAANNNNTTC 1 cut(s) 424
PfeI GAWTC 2 cut(s) 517, 663
PflMI CCANNNNNTGG 1 cut(s) 708
PkrI GCNGC 4 cut(s) 296, 514, 595, 808
PpuMI RGGWCCY 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 305
Psp6I CCWGG 1 cut(s) 301
PspGI CCWGG 1 cut(s) 301
PspN4I GGNNCC 3 cut(s) 421, 676, 818
PspPI GGNCC 3 cut(s) 80, 305, 609
PspPPI RGGWCCY 1 cut(s) 305
PstNI CAGNNNCTG 2 cut(s) 227, 308
PsuI RGATCY 2 cut(s) 172, 816
RsaI GTAC 2 cut(s) 582, 868
RsaNI GTAC 2 cut(s) 581, 867
SapI GCTCTTC 1 cut(s) 843
SaqAI TTAA 3 cut(s) 321, 342, 650
SatI GCNGC 4 cut(s) 295, 513, 594, 807
Sau3AI GATC 3 cut(s) 172, 816, 894
Sau96I GGNCC 3 cut(s) 80, 305, 609
ScrFI CCNGG 2 cut(s) 50, 303
SfaNI GCATC 2 cut(s) 565, 569
SfuI TTCGAA 2 cut(s) 73, 123
SinI GGWCC 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 854
SmoI CTYRAG 1 cut(s) 854
Sse9I AATT 3 cut(s) 69, 404, 886
SsiI CCGC 3 cut(s) 65, 294, 688
SspMI CTAG 3 cut(s) 365, 546, 693
StyD4I CCNGG 2 cut(s) 48, 301
StyI CCWWGG 1 cut(s) 707
TaaI ACNGT 1 cut(s) 181
TaqI TCGA 3 cut(s) 73, 123, 893
TaqII GACCGA 1 cut(s) 866
TasI AATT 3 cut(s) 69, 404, 886
TatI WGTACW 2 cut(s) 580, 866
TauI GCSGC 1 cut(s) 297
TfiI GAWTC 2 cut(s) 517, 663
Tru1I TTAA 3 cut(s) 321, 342, 650
Tru9I TTAA 3 cut(s) 321, 342, 650
TscAI CASTG 3 cut(s) 13, 476, 608
TseI GCWGC 3 cut(s) 512, 593, 806
TspDTI ATGAA 5 cut(s) 75, 247, 327, 741, 904
TspGWI ACGGA 1 cut(s) 857
TspRI CASTG 3 cut(s) 13, 476, 608
Van91I CCANNNNNTGG 1 cut(s) 708
VpaK11BI GGWCC 1 cut(s) 305
XagI CCTNNNNNAGG 1 cut(s) 351
XapI RAATTY 1 cut(s) 404
XceI RCATGY 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 3 cut(s) 365, 546, 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.