MD02G1016900.v1.1

methyltransferase At2g41040, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002821
Physical Location & Seq
Forward (+)
1262258 .. 1265336
3079 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1016900.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCGACGACACCCTCCTCCACTCTCCACCAAAAGTTTCTATCCCTAAACTATCCTCCCTTCCATCACAATTCTCAATTCCGCCCTCCCCGTCTCCGCCGCCCCTCCCGCCATCACTGTCACCCCATTCGCGCCACCTCCACCGTCGCTCTCCCGCCGGAGCGGCGGTCGAGGGCCGAGGAAATCCAAAGCTCCGGCGTTGACCTCGAGGTGCTTGCCTGCCCAATATGCTACGAACCACTCATCCGAAGGGGGCCGCCGGGTCTCAATCTGCGAGCAATTTACAGGTCGGGGTTCAAGTGCAGGAAATGCGACAAGACGTATTCGAGCAAAGACGTTTACTTGGACCTTACTGTTACTGCTGGGTTGAAGGAGTACGTTGAGGTACAACCGACCCGGACCGAGTTGTTCCGGAGCCCTCTCGTTTCCTTCTTGTATGAAAGGGGTTGGCGCCAGAATTTTAACCGCAGCGGATTTCCGGGTCCCGATGAAGAGTTTAAGATGGCTCAGGACTACTTTAAATCCGCCGAAGGCGGTGTTTTGGTGGATGTCAGCTGTGGGAGCGGCTTGTTTTCGAGGAAGTTTGCGAAATCCGGGACATATTCTGCAGTTGTTGCACTTGATTTTTCGGAGAACATGCTTCGGCAGAGTTATGAATTTTTCAAGAGAGATCCTACTATTTTAGATACTAATCTTGCTCTTGTGAGGGCGGATGTTGCTAGGCTTCCGTTCCCTTCGGGTTCAGTTGATGCTGTCCATGCCGGCGCAGCATTGCATTGCTGGCCCTCTCCTTCCAATGCTATTGCTGAAATAACTCGCATATTACGAAGTGGTGGTGTCTTTGTTGGAACCACTTTTCTGCGGTATACTCCATCTACTCCCTGGATAACCCGGCAACTCAGAGAGAGTGCTCTTCAGGGCTACAACTATTTAACGGAGGAGGAGATCGAGGACTTGTGTGCATCATGCGGTCTCACCAACTACACGAGCAACGTTCAACAAGCTTTCATCATGTTTTCTGCTCAGAAACCTTAA

Protein Analysis

345

Amino Acids

38.7

Weight (kDa)

9.01

Isoelectric Point (pI)

60.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 173 - 285 1.4e-10 ubiE/COQ5 methyltransferase family
Methyltransf_23 PF13489 175 - 328 1.9e-11 Methyltransferase domain
Methyltransf_31 PF13847 178 - 287 9.3e-12 Methyltransferase domain
Methyltransf_11 PF08241 182 - 282 4.1e-21 Methyltransferase domain
Methyltransf_25 PF13649 182 - 279 3.2e-17 Methyltransferase domain
Methyltransf_12 PF08242 182 - 281 2.5e-11 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 206
AccB1I GGYRCC 1 cut(s) 450
AccBSI CCGCTC 2 cut(s) 163, 564
AccI GTMKAC 1 cut(s) 866
AccII CGCG 1 cut(s) 132
AccIII TCCGGA 1 cut(s) 411
AclI AACGTT 1 cut(s) 993
AclWI GGATC 1 cut(s) 665
AcsI RAATTY 2 cut(s) 457, 656
AcuI CTGAAG 1 cut(s) 899
AcyI GRCGYC 1 cut(s) 451
AfaI GTAC 2 cut(s) 377, 387
AgsI TTSAA 4 cut(s) 298, 370, 664, 998
AjnI CCWGG 1 cut(s) 881
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
AluBI AGCT 3 cut(s) 192, 555, 1004
AluI AGCT 3 cut(s) 192, 555, 1004
Alw21I GWGCWC 1 cut(s) 913
Alw26I GTCTC 3 cut(s) 98, 269, 977
AlwI GGATC 1 cut(s) 665
Ama87I CYCGRG 1 cut(s) 206
Aor13HI TCCGGA 1 cut(s) 411
AoxI GGCC 3 cut(s) 174, 254, 782
ApeKI GCWGC 2 cut(s) 468, 767
ApoI RAATTY 2 cut(s) 457, 656
AspLEI GCGC 3 cut(s) 134, 453, 767
AspS9I GGNCC 6 cut(s) 174, 254, 346, 399, 482, 783
AsuC2I CCSGG 5 cut(s) 261, 397, 480, 595, 892
AsuHPI GGTGA 2 cut(s) 113, 967
AvaI CYCGRG 1 cut(s) 206
AvaII GGWCC 3 cut(s) 346, 399, 482
BanI GGYRCC 1 cut(s) 450
BanII GRGCYC 1 cut(s) 419
BauI CACGAG 1 cut(s) 985
Bbv12I GWGCWC 1 cut(s) 913
BbvI GCAGC 2 cut(s) 480, 779
BccI CCATC 4 cut(s) 72, 120, 496, 880
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 1 cut(s) 883
BcnI CCSGG 5 cut(s) 261, 397, 480, 595, 892
BcoDI GTCTC 3 cut(s) 98, 269, 977
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 1 cut(s) 606
BfoI RGCGCY 1 cut(s) 454
BglI GCCNNNNNGGC 1 cut(s) 163
BisI GCNGC 6 cut(s) 100, 164, 257, 469, 565, 768
BlsI GCNGC 6 cut(s) 101, 165, 258, 470, 566, 769
Bme1390I CCNGG 6 cut(s) 261, 397, 480, 595, 883, 892
Bme18I GGWCC 3 cut(s) 346, 399, 482
BmeT110I CYCGRG 1 cut(s) 206
BmgT120I GGNCC 6 cut(s) 174, 254, 346, 399, 482, 783
BmiI GGNNCC 6 cut(s) 255, 416, 452, 483, 484, 850
BmrFI CCNGG 6 cut(s) 261, 397, 480, 595, 883, 892
BmsI GCATC 2 cut(s) 739, 971
Bpu10I CCTNAGC 1 cut(s) 507
BpuMI CCSGG 5 cut(s) 261, 397, 480, 595, 892
BsaBI GATNNNNATC 1 cut(s) 690
BsaHI GRCGYC 1 cut(s) 451
BsaI GGTCTC 2 cut(s) 269, 977
BsaJI CCNNGG 2 cut(s) 177, 881
BsaWI WCCGGW 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bse118I RCCGGY 1 cut(s) 761
Bse3DI GCAATG 2 cut(s) 770, 775
Bse8I GATNNNNATC 1 cut(s) 690
BseAI TCCGGA 1 cut(s) 411
BseBI CCWGG 1 cut(s) 883
BseDI CCNNGG 2 cut(s) 177, 881
BseGI GGATG 3 cut(s) 243, 553, 718
BseJI GATNNNNATC 1 cut(s) 690
BseMI GCAATG 2 cut(s) 770, 775
BseMII CTCAG 2 cut(s) 521, 913
BseRI GAGGAG 3 cut(s) 7, 953, 956
BseXI GCAGC 2 cut(s) 480, 779
BseYI CCCAGC 1 cut(s) 362
BsgI GTGCAG 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 132
Bsh1285I CGRYCG 1 cut(s) 170
BshFI GGCC 3 cut(s) 176, 256, 784
BshNI GGYRCC 1 cut(s) 450
BsiEI CGRYCG 1 cut(s) 170
BsiHKAI GWGCWC 1 cut(s) 913
BsiHKCI CYCGRG 1 cut(s) 206
BsiSI CCGG 9 cut(s) 158, 195, 260, 397, 412, 479, 594, 762, 892
BslFI GGGAC 2 cut(s) 468, 610
BsmAI GTCTC 3 cut(s) 98, 269, 977
BsmBI CGTCTC 1 cut(s) 98
BsmFI GGGAC 2 cut(s) 468, 610
BsnI GGCC 3 cut(s) 176, 256, 784
Bso31I GGTCTC 2 cut(s) 269, 977
BsoBI CYCGRG 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 419, 913
Bsp13I TCCGGA 1 cut(s) 411
Bsp143I GATC 2 cut(s) 670, 945
BspANI GGCC 3 cut(s) 176, 256, 784
BspCNI CTCAG 2 cut(s) 520, 912
BspEI TCCGGA 1 cut(s) 411
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 6 cut(s) 255, 416, 452, 483, 484, 850
BspMAI CTGCAG 1 cut(s) 610
BspPI GGATC 1 cut(s) 665
BspQI GCTCTTC 1 cut(s) 918
BspT107I GGYRCC 1 cut(s) 450
BspTNI GGTCTC 2 cut(s) 269, 977
BsrBI CCGCTC 2 cut(s) 163, 564
BsrDI GCAATG 2 cut(s) 770, 775
BsrFI RCCGGY 1 cut(s) 761
BssAI RCCGGY 1 cut(s) 761
BssECI CCNNGG 2 cut(s) 177, 881
BssMI GATC 2 cut(s) 670, 945
BssNAI GTATAC 1 cut(s) 867
BssNI GRCGYC 1 cut(s) 451
BssSI CACGAG 1 cut(s) 985
Bst1107I GTATAC 1 cut(s) 867
Bst2BI CACGAG 1 cut(s) 985
Bst2UI CCWGG 1 cut(s) 883
Bst4CI ACNGT 3 cut(s) 119, 145, 355
Bst6I CTCTTC 2 cut(s) 486, 918
BstACI GRCGYC 1 cut(s) 451
BstAPI GCANNNNNTGC 2 cut(s) 309, 614
BstC8I GCNNGC 5 cut(s) 216, 220, 276, 763, 782
BstDEI CTNAG 3 cut(s) 507, 899, 1023
BstF5I GGATG 3 cut(s) 243, 553, 718
BstFNI CGCG 1 cut(s) 132
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 3 cut(s) 134, 453, 767
BstKTI GATC 2 cut(s) 673, 948
BstMAI GTCTC 3 cut(s) 98, 269, 977
BstMBI GATC 2 cut(s) 670, 945
BstMCI CGRYCG 1 cut(s) 170
BstNI CCWGG 1 cut(s) 883
BstNSI RCATGY 1 cut(s) 640
BstSCI CCNGG 6 cut(s) 259, 395, 478, 593, 881, 890
BstSFI CTRYAG 1 cut(s) 606
BstUI CGCG 1 cut(s) 132
BstV1I GCAGC 2 cut(s) 480, 779
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BstZ17I GTATAC 1 cut(s) 867
BsuRI GGCC 3 cut(s) 176, 256, 784
BtsCI GGATG 3 cut(s) 243, 553, 718
BtsIMutI CAGTG 1 cut(s) 115
Cac8I GCNNGC 5 cut(s) 216, 220, 276, 763, 782
CfoI GCGC 3 cut(s) 134, 453, 767
Cfr10I RCCGGY 1 cut(s) 761
Cfr13I GGNCC 6 cut(s) 174, 254, 346, 399, 482, 783
CpoI CGGWCCG 1 cut(s) 399
Csp6I GTAC 2 cut(s) 376, 386
CspI CGGWCCG 1 cut(s) 399
CviAII CATG 4 cut(s) 637, 758, 966, 1012
CviQI GTAC 2 cut(s) 376, 386
DdeI CTNAG 3 cut(s) 507, 899, 1023
DinI GGCGCC 1 cut(s) 452
DpnI GATC 2 cut(s) 672, 947
DpnII GATC 2 cut(s) 670, 945
DraI TTTAAA 1 cut(s) 520
Eam1104I CTCTTC 2 cut(s) 486, 918
EarI CTCTTC 2 cut(s) 486, 918
EciI GGCGGA 4 cut(s) 71, 86, 514, 725
Eco24I GRGCYC 1 cut(s) 419
Eco31I GGTCTC 2 cut(s) 269, 977
Eco47I GGWCC 3 cut(s) 346, 399, 482
Eco57I CTGAAG 1 cut(s) 899
Eco88I CYCGRG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 482
EcoRII CCWGG 1 cut(s) 881
EcoT38I GRGCYC 1 cut(s) 419
EgeI GGCGCC 1 cut(s) 452
EheI GGCGCC 1 cut(s) 452
Esp3I CGTCTC 1 cut(s) 98
FaeI CATG 4 cut(s) 640, 761, 969, 1015
FaqI GGGAC 2 cut(s) 468, 610
FatI CATG 4 cut(s) 636, 757, 965, 1011
FauI CCCGC 2 cut(s) 116, 162
FblI GTMKAC 1 cut(s) 866
Fnu4HI GCNGC 6 cut(s) 100, 164, 257, 469, 565, 768
FokI GGATG 3 cut(s) 230, 560, 725
FriOI GRGCYC 1 cut(s) 419
Fsp4HI GCNGC 6 cut(s) 100, 164, 257, 469, 565, 768
FspBI CTAG 1 cut(s) 720
GlaI GCGC 3 cut(s) 133, 452, 766
GluI GCNGC 6 cut(s) 100, 164, 257, 469, 565, 768
GsaI CCCAGC 1 cut(s) 366
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 3 cut(s) 176, 256, 784
HapII CCGG 9 cut(s) 158, 195, 260, 397, 412, 479, 594, 762, 892
HhaI GCGC 3 cut(s) 134, 453, 767
Hin1I GRCGYC 1 cut(s) 451
Hin1II CATG 4 cut(s) 640, 761, 969, 1015
Hin6I GCGC 3 cut(s) 132, 451, 765
HinP1I GCGC 3 cut(s) 132, 451, 765
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 1002
HpaII CCGG 9 cut(s) 158, 195, 260, 397, 412, 479, 594, 762, 892
HphI GGTGA 2 cut(s) 113, 967
Hpy166II GTNNAC 3 cut(s) 202, 340, 867
Hpy188I TCNGA 4 cut(s) 248, 631, 902, 1026
Hpy188III TCNNGA 4 cut(s) 412, 485, 509, 664
Hpy8I GTNNAC 3 cut(s) 202, 340, 867
Hpy99I CGWCG 2 cut(s) 10, 149
HpyAV CCTTC 7 cut(s) 70, 243, 364, 439, 524, 744, 801
HpyCH4III ACNGT 3 cut(s) 119, 145, 355
HpyCH4IV ACGT 4 cut(s) 320, 336, 378, 993
HpyCH4V TGCA 5 cut(s) 303, 608, 617, 775, 962
HpyF3I CTNAG 3 cut(s) 507, 899, 1023
HpySE526I ACGT 4 cut(s) 320, 336, 378, 993
Hsp92I GRCGYC 1 cut(s) 451
Hsp92II CATG 4 cut(s) 640, 761, 969, 1015
HspAI GCGC 3 cut(s) 132, 451, 765
KasI GGCGCC 1 cut(s) 450
KflI GGGWCCC 1 cut(s) 482
Kpn2I TCCGGA 1 cut(s) 411
KroI GCCGGC 1 cut(s) 761
KroNI GCCGGC 1 cut(s) 763
Kzo9I GATC 2 cut(s) 670, 945
LguI GCTCTTC 1 cut(s) 918
LmnI GCTCC 4 cut(s) 160, 197, 414, 561
Lsp1109I GCAGC 2 cut(s) 480, 779
LweI GCATC 2 cut(s) 739, 971
MaeI CTAG 1 cut(s) 720
MaeII ACGT 4 cut(s) 320, 336, 378, 993
MaeIII GTNAC 2 cut(s) 119, 355
MalI GATC 2 cut(s) 672, 947
MbiI CCGCTC 2 cut(s) 163, 564
MboI GATC 2 cut(s) 670, 945
MboII GAAGA 2 cut(s) 503, 905
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 2 cut(s) 419, 913
MluCI AATT 5 cut(s) 70, 77, 279, 457, 656
Mly113I GGCGCC 1 cut(s) 451
MmeI TCCRAC 1 cut(s) 826
MroI TCCGGA 1 cut(s) 411
MroNI GCCGGC 1 cut(s) 761
MseI TTAA 5 cut(s) 462, 498, 519, 932, 1033
MspA1I CMGCKG 2 cut(s) 471, 555
MspI CCGG 9 cut(s) 158, 195, 260, 397, 412, 479, 594, 762, 892
MspR9I CCNGG 6 cut(s) 261, 397, 480, 595, 883, 892
MvaI CCWGG 1 cut(s) 883
MvnI CGCG 1 cut(s) 132
NaeI GCCGGC 1 cut(s) 763
NarI GGCGCC 1 cut(s) 451
NciI CCSGG 5 cut(s) 261, 397, 480, 595, 892
NdeII GATC 2 cut(s) 670, 945
NgoMIV GCCGGC 1 cut(s) 761
NlaIII CATG 4 cut(s) 640, 761, 969, 1015
NlaIV GGNNCC 6 cut(s) 255, 416, 452, 483, 484, 850
NmeAIII GCCGAG 1 cut(s) 202
NmuCI GTSAC 1 cut(s) 119
NspI RCATGY 1 cut(s) 640
PaeR7I CTCGAG 1 cut(s) 206
PciSI GCTCTTC 1 cut(s) 918
PcsI WCGNNNNNNNCGW 1 cut(s) 823
PdiI GCCGGC 1 cut(s) 763
PfoI TCCNGGA 1 cut(s) 593
PkrI GCNGC 6 cut(s) 101, 165, 258, 470, 566, 769
PluTI GGCGCC 1 cut(s) 454
PpuMI RGGWCCY 1 cut(s) 482
Psp1406I AACGTT 1 cut(s) 993
Psp5II RGGWCCY 1 cut(s) 482
Psp6I CCWGG 1 cut(s) 881
PspFI CCCAGC 1 cut(s) 362
PspGI CCWGG 1 cut(s) 881
PspN4I GGNNCC 6 cut(s) 255, 416, 452, 483, 484, 850
PspPI GGNCC 6 cut(s) 174, 254, 346, 399, 482, 783
PspPPI RGGWCCY 1 cut(s) 482
PspXI VCTCGAGB 1 cut(s) 206
PstI CTGCAG 1 cut(s) 610
PsuI RGATCY 1 cut(s) 670
PvuII CAGCTG 1 cut(s) 555
RsaI GTAC 2 cut(s) 377, 387
RsaNI GTAC 2 cut(s) 376, 386
Rsr2I CGGWCCG 1 cut(s) 399
RsrII CGGWCCG 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 918
SaqAI TTAA 5 cut(s) 462, 498, 519, 932, 1033
SatI GCNGC 6 cut(s) 100, 164, 257, 469, 565, 768
Sau3AI GATC 2 cut(s) 670, 945
Sau96I GGNCC 6 cut(s) 174, 254, 346, 399, 482, 783
ScrFI CCNGG 6 cut(s) 261, 397, 480, 595, 883, 892
SduI GDGCHC 2 cut(s) 419, 913
SfaNI GCATC 2 cut(s) 739, 971
SfcI CTRYAG 1 cut(s) 606
SfoI GGCGCC 1 cut(s) 452
Sfr274I CTCGAG 1 cut(s) 206
SinI GGWCC 3 cut(s) 346, 399, 482
SlaI CTCGAG 1 cut(s) 206
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 5 cut(s) 70, 77, 279, 457, 656
SspDI GGCGCC 1 cut(s) 450
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 6 cut(s) 259, 395, 478, 593, 881, 890
TaaI ACNGT 3 cut(s) 119, 145, 355
TaiI ACGT 4 cut(s) 323, 339, 381, 996
TaqI TCGA 5 cut(s) 170, 207, 326, 575, 948
TaqII GACCGA 1 cut(s) 416
TasI AATT 5 cut(s) 70, 77, 279, 457, 656
TauI GCSGC 4 cut(s) 102, 166, 259, 567
Tru1I TTAA 5 cut(s) 462, 498, 519, 932, 1033
Tru9I TTAA 5 cut(s) 462, 498, 519, 932, 1033
TscAI CASTG 1 cut(s) 122
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 2 cut(s) 468, 767
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 4 cut(s) 453, 504, 669, 997
TspGWI ACGGA 2 cut(s) 717, 950
TspRI CASTG 1 cut(s) 122
VpaK11BI GGWCC 3 cut(s) 346, 399, 482
XapI RAATTY 2 cut(s) 457, 656
XceI RCATGY 1 cut(s) 640
XcmI CCANNNNNNNNNTGG 1 cut(s) 879
XhoI CTCGAG 1 cut(s) 206
XmiI GTMKAC 1 cut(s) 866
XspI CTAG 1 cut(s) 720
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.