FvH4_1g02550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003259
Physical Location & Seq
Forward (+)
1430818 .. 1433114
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02550.t1

Sequence Viewer

Length: 1083 bp
ATGTCTTGGCTCTTCACCCCTCTCCAACCCGACGGGGAAAACGACTCGTCGTTTCTCTCCCAAACCCTCACCCGCCAACTCCGCGGCGTCGCCACCTTCCTCGCTCCGCCTCCGCCGTCATCCTCCTCCTCCGATTCTCCACCGCCGTCGCTGGCGCTCTTGGGAGTCCGAAACGATCTGGCGGAATTTGGCGGGAGCTTGAAGACCGGCCTCTCCCTTCTCTCCGTCGCCGGAATCTCCAAATTGGCTTCGAACTTGCTGCCGGTTCGGAACCGAGACGGCGGAGGTTCCGTGCCGGGGATCACCGATGAGATTCTCGGCTTCGTCGCTGAGATTTCGAAGCTTCCGGACTGTTGGACCGGCTTTCCTATACCACTGGATCACCATGATTTTAGCATGTCCAGTGCTCAGAGAGAGCATGCCTCCGCTGTCGAAGAGTTGGTTCCGGAGTTTGTGGAATTAAGAGTCAGAGTTTGCGGTTCGATGAGCAAGGATCAATTTTGGATGATATATTTCTTGCTGCTGCTGCCAAGAATAAATGCAGATGATTTTGAGCTTCTGTCGACCCCCAAGATTGTTGAAGCAAGAGATGCACTTTTGCACAAGCTTCAAAAGGACACACAGGTATCTGAGGAGTCAACTCTTGATTCAACTGAAAAGGACACCCTGGTTGAGAAGACGCAAGGGGAGAAAGTCTCGTCTTTAGAAAAGAAAGATTTGACAGAGATTGTAAATACAGCAGAAAGGTTAAGAATCGACGATGAAGAGAGCACTGAGCAATGGTCCGAAGAGGCAAGTATTAGTTCAGGTACTTTTGTGGATGACCCAAAGAAACTTGAACATGAAGATGATATCTCATTCAGCGATCTAGAGGGTAACGAGAGTGACATTTCTAGTAGATCATCAGGCCTAAGACCAGCTCAGGCCATTAGGGGATGTTCACCAAGTGGATCAAATGACTGGGTTCGGCTGAATAGAAGTTCTGCAATTGGTAGTGGCCATCAAAGGACTGGTACGTTGAAAGAGAAAGATTCAGAAGGTGAAGAGTCGAATGACTGGCTCACTGTAGATAAGTTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

361

Amino Acids

39.44

Weight (kDa)

4.57

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 563
AccII CGCG 1 cut(s) 84
AccIII TCCGGA 2 cut(s) 346, 445
AclWI GGATC 4 cut(s) 308, 387, 501, 958
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 1 cut(s) 185
AcyI GRCGYC 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 947
AfaI GTAC 2 cut(s) 811, 1015
AfiI CCNNNNNNNGG 1 cut(s) 297
AgsI TTSAA 6 cut(s) 202, 581, 611, 651, 839, 1021
AjnI CCWGG 1 cut(s) 666
AluBI AGCT 5 cut(s) 198, 343, 556, 607, 920
AluI AGCT 5 cut(s) 198, 343, 556, 607, 920
Alw21I GWGCWC 2 cut(s) 409, 773
Alw26I GTCTC 2 cut(s) 270, 700
AlwI GGATC 4 cut(s) 308, 387, 501, 958
Aor13HI TCCGGA 2 cut(s) 346, 445
AoxI GGCC 4 cut(s) 208, 907, 924, 997
ApeKI GCWGC 4 cut(s) 259, 520, 523, 526
ApoI RAATTY 1 cut(s) 185
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 2 cut(s) 357, 783
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 6 cut(s) 7, 61, 295, 374, 933, 1052
AsuII TTCGAA 2 cut(s) 251, 338
AvaII GGWCC 2 cut(s) 357, 783
BaeI ACNNNNGTAYC 4 cut(s) 609, 642, 801, 834
BalI TGGCCA 1 cut(s) 999
BbsI GAAGAC 2 cut(s) 209, 683
Bbv12I GWGCWC 2 cut(s) 409, 773
BbvI GCAGC 4 cut(s) 246, 507, 510, 513
BccI CCATC 1 cut(s) 1008
BceAI ACGGC 3 cut(s) 100, 130, 295
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 668
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 2 cut(s) 270, 700
BfaI CTAG 2 cut(s) 869, 894
BfmI CTRYAG 1 cut(s) 1065
BfoI RGCGCY 1 cut(s) 158
BisI GCNGC 5 cut(s) 85, 260, 521, 524, 527
BlsI GCNGC 5 cut(s) 86, 261, 522, 525, 528
Bme1390I CCNGG 2 cut(s) 297, 668
Bme18I GGWCC 2 cut(s) 357, 783
BmgT120I GGNCC 2 cut(s) 357, 783
BmiI GGNNCC 3 cut(s) 272, 289, 444
BmrFI CCNGG 2 cut(s) 297, 668
BmrI ACTGGG 1 cut(s) 970
BmsI GCATC 1 cut(s) 580
BmuI ACTGGG 1 cut(s) 970
BpiI GAAGAC 2 cut(s) 209, 683
BplI GAGNNNNNCTC 4 cut(s) 407, 439, 680, 712
Bpu10I CCTNAGC 1 cut(s) 921
Bpu14I TTCGAA 2 cut(s) 251, 338
BpuMI CCSGG 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 3 cut(s) 82, 296, 666
BsaWI WCCGGW 2 cut(s) 346, 445
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 1 cut(s) 297
Bse118I RCCGGY 3 cut(s) 206, 262, 359
Bse1I ACTGG 5 cut(s) 381, 402, 965, 1015, 1061
Bse3DI GCAATG 1 cut(s) 785
BseAI TCCGGA 2 cut(s) 346, 445
BseBI CCWGG 1 cut(s) 668
BseDI CCNNGG 3 cut(s) 82, 296, 666
BseGI GGATG 4 cut(s) 119, 510, 826, 941
BseLI CCNNNNNNNGG 1 cut(s) 297
BseMI GCAATG 1 cut(s) 785
BseMII CTCAG 5 cut(s) 321, 422, 621, 765, 935
BseNI ACTGG 5 cut(s) 381, 402, 965, 1015, 1061
BseRI GAGGAG 3 cut(s) 115, 118, 647
BseXI GCAGC 4 cut(s) 246, 507, 510, 513
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 210, 909, 926, 999
BsiHKAI GWGCWC 2 cut(s) 409, 773
BsiSI CCGG 7 cut(s) 207, 231, 263, 296, 347, 360, 446
BslI CCNNNNNNNGG 1 cut(s) 297
BsmAI GTCTC 2 cut(s) 270, 700
BsmBI CGTCTC 1 cut(s) 270
BsnI GGCC 4 cut(s) 210, 909, 926, 999
Bsp119I TTCGAA 2 cut(s) 251, 338
Bsp1286I GDGCHC 2 cut(s) 409, 773
Bsp13I TCCGGA 2 cut(s) 346, 445
Bsp143I GATC 7 cut(s) 175, 300, 379, 493, 865, 899, 950
BspANI GGCC 4 cut(s) 210, 909, 926, 999
BspCNI CTCAG 5 cut(s) 322, 421, 622, 766, 934
BspEI TCCGGA 2 cut(s) 346, 445
BspFNI CGCG 1 cut(s) 84
BspLI GGNNCC 3 cut(s) 272, 289, 444
BspPI GGATC 4 cut(s) 308, 387, 501, 958
BspQI GCTCTTC 1 cut(s) 17
BspT104I TTCGAA 2 cut(s) 251, 338
BsrDI GCAATG 1 cut(s) 785
BsrFI RCCGGY 3 cut(s) 206, 262, 359
BsrI ACTGG 5 cut(s) 381, 402, 965, 1015, 1061
BssAI RCCGGY 3 cut(s) 206, 262, 359
BssECI CCNNGG 3 cut(s) 82, 296, 666
BssMI GATC 7 cut(s) 175, 300, 379, 493, 865, 899, 950
BssNI GRCGYC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 668
Bst4CI ACNGT 2 cut(s) 353, 1066
Bst6I CTCTTC 5 cut(s) 17, 429, 759, 783, 1038
BstACI GRCGYC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 590
BstBI TTCGAA 2 cut(s) 251, 338
BstC8I GCNNGC 2 cut(s) 153, 420
BstDEI CTNAG 6 cut(s) 330, 408, 630, 774, 911, 921
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 4 cut(s) 119, 510, 826, 941
BstFNI CGCG 1 cut(s) 84
BstH2I RGCGCY 1 cut(s) 158
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 7 cut(s) 178, 303, 382, 496, 868, 902, 953
BstMAI GTCTC 2 cut(s) 270, 700
BstMBI GATC 7 cut(s) 175, 300, 379, 493, 865, 899, 950
BstMWI GCNNNNNNNGC 3 cut(s) 81, 526, 590
BstNI CCWGG 1 cut(s) 668
BstNSI RCATGY 2 cut(s) 400, 422
BstSCI CCNGG 2 cut(s) 295, 666
BstSFI CTRYAG 1 cut(s) 1065
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 4 cut(s) 246, 507, 510, 513
BstV2I GAAGAC 2 cut(s) 209, 683
BsuRI GGCC 4 cut(s) 210, 909, 926, 999
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 4 cut(s) 119, 510, 826, 941
BtsIMutI CAGTG 4 cut(s) 374, 409, 771, 1062
Cac8I GCNNGC 2 cut(s) 153, 420
CfoI GCGC 1 cut(s) 157
Cfr10I RCCGGY 3 cut(s) 206, 262, 359
Cfr13I GGNCC 2 cut(s) 357, 783
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 2 cut(s) 76, 688
Csp6I GTAC 2 cut(s) 810, 1014
CviAII CATG 4 cut(s) 386, 397, 419, 842
CviQI GTAC 2 cut(s) 810, 1014
DdeI CTNAG 6 cut(s) 330, 408, 630, 774, 911, 921
DpnI GATC 7 cut(s) 177, 302, 381, 495, 867, 901, 952
DpnII GATC 7 cut(s) 175, 300, 379, 493, 865, 899, 950
DraIII CACNNNGTG 1 cut(s) 947
EaeI YGGCCR 1 cut(s) 997
Eam1104I CTCTTC 5 cut(s) 17, 429, 759, 783, 1038
EarI CTCTTC 5 cut(s) 17, 429, 759, 783, 1038
EciI GGCGGA 4 cut(s) 96, 102, 197, 297
Eco147I AGGCCT 1 cut(s) 909
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 2 cut(s) 357, 783
EcoRII CCWGG 1 cut(s) 666
EcoRV GATATC 1 cut(s) 853
Esp3I CGTCTC 1 cut(s) 270
FaeI CATG 4 cut(s) 389, 400, 422, 845
FaiI YATR 6 cut(s) 371, 387, 398, 420, 511, 843
FatI CATG 4 cut(s) 385, 396, 418, 841
FauI CCCGC 2 cut(s) 80, 185
FblI GTMKAC 1 cut(s) 563
Fnu4HI GCNGC 5 cut(s) 85, 260, 521, 524, 527
FokI GGATG 4 cut(s) 106, 517, 833, 948
Fsp4HI GCNGC 5 cut(s) 85, 260, 521, 524, 527
FspBI CTAG 2 cut(s) 869, 894
GlaI GCGC 1 cut(s) 156
GluI GCNGC 5 cut(s) 85, 260, 521, 524, 527
HaeII RGCGCY 1 cut(s) 158
HaeIII GGCC 4 cut(s) 210, 909, 926, 999
HapII CCGG 7 cut(s) 207, 231, 263, 296, 347, 360, 446
HgaI GACGC 2 cut(s) 76, 688
HhaI GCGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 4 cut(s) 389, 400, 422, 845
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HincII GTYRAC 2 cut(s) 564, 639
HindII GTYRAC 2 cut(s) 564, 639
HindIII AAGCTT 2 cut(s) 341, 605
HpaII CCGG 7 cut(s) 207, 231, 263, 296, 347, 360, 446
HphI GGTGA 6 cut(s) 7, 61, 295, 374, 933, 1052
Hpy166II GTNNAC 3 cut(s) 564, 639, 941
Hpy188I TCNGA 8 cut(s) 133, 170, 270, 411, 470, 631, 787, 1036
Hpy188III TCNNGA 4 cut(s) 347, 446, 644, 869
Hpy8I GTNNAC 3 cut(s) 564, 639, 941
Hpy99I CGWCG 7 cut(s) 35, 52, 92, 151, 230, 329, 761
HpyAV CCTTC 3 cut(s) 106, 227, 1031
HpyCH4III ACNGT 2 cut(s) 353, 1066
HpyCH4IV ACGT 1 cut(s) 1016
HpyCH4V TGCA 4 cut(s) 542, 593, 601, 986
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 526, 590
HpyF3I CTNAG 6 cut(s) 330, 408, 630, 774, 911, 921
HpySE526I ACGT 1 cut(s) 1016
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 4 cut(s) 389, 400, 422, 845
HspAI GCGC 1 cut(s) 155
Kpn2I TCCGGA 2 cut(s) 346, 445
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 7 cut(s) 175, 300, 379, 493, 865, 899, 950
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 2 cut(s) 109, 195
Lsp1109I GCAGC 4 cut(s) 246, 507, 510, 513
LweI GCATC 1 cut(s) 580
MaeI CTAG 2 cut(s) 869, 894
MaeII ACGT 1 cut(s) 1016
MaeIII GTNAC 2 cut(s) 875, 884
MalI GATC 7 cut(s) 177, 302, 381, 495, 867, 901, 952
MboI GATC 7 cut(s) 175, 300, 379, 493, 865, 899, 950
MboII GAAGA 8 cut(s) 4, 214, 446, 688, 776, 800, 857, 1055
MfeI CAATTG 1 cut(s) 987
MhlI GDGCHC 2 cut(s) 409, 773
MlsI TGGCCA 1 cut(s) 999
MluCI AATT 5 cut(s) 185, 242, 458, 497, 987
MluNI TGGCCA 1 cut(s) 999
MlyI GAGTC 5 cut(s) 38, 174, 474, 644, 1055
MmeI TCCRAC 2 cut(s) 49, 335
Mox20I TGGCCA 1 cut(s) 999
MroI TCCGGA 2 cut(s) 346, 445
MscI TGGCCA 1 cut(s) 999
MseI TTAA 2 cut(s) 461, 749
MslI CAYNNNNRTG 1 cut(s) 846
Msp20I TGGCCA 1 cut(s) 999
MspA1I CMGCKG 2 cut(s) 84, 428
MspI CCGG 7 cut(s) 207, 231, 263, 296, 347, 360, 446
MspR9I CCNGG 2 cut(s) 297, 668
MunI CAATTG 1 cut(s) 987
MvaI CCWGG 1 cut(s) 668
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 81, 526, 590
NciI CCSGG 1 cut(s) 297
NdeII GATC 7 cut(s) 175, 300, 379, 493, 865, 899, 950
NlaIII CATG 4 cut(s) 389, 400, 422, 845
NlaIV GGNNCC 3 cut(s) 272, 289, 444
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 884
NspI RCATGY 2 cut(s) 400, 422
NspV TTCGAA 2 cut(s) 251, 338
PaeI GCATGC 1 cut(s) 422
PceI AGGCCT 1 cut(s) 909
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 6 cut(s) 134, 234, 313, 647, 753, 1031
PkrI GCNGC 5 cut(s) 86, 261, 522, 525, 528
PleI GAGTC 5 cut(s) 38, 173, 473, 643, 1054
PpsI GAGTC 5 cut(s) 38, 173, 473, 643, 1054
Psp6I CCWGG 1 cut(s) 666
PspGI CCWGG 1 cut(s) 666
PspN4I GGNNCC 3 cut(s) 272, 289, 444
PspPI GGNCC 2 cut(s) 357, 783
RsaI GTAC 2 cut(s) 811, 1015
RsaNI GTAC 2 cut(s) 810, 1014
RseI CAYNNNNRTG 1 cut(s) 846
SacII CCGCGG 1 cut(s) 85
SalI GTCGAC 1 cut(s) 562
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 2 cut(s) 461, 749
SatI GCNGC 5 cut(s) 85, 260, 521, 524, 527
Sau3AI GATC 7 cut(s) 175, 300, 379, 493, 865, 899, 950
Sau96I GGNCC 2 cut(s) 357, 783
SchI GAGTC 5 cut(s) 38, 174, 474, 644, 1055
ScrFI CCNGG 2 cut(s) 297, 668
SduI GDGCHC 2 cut(s) 409, 773
SfaNI GCATC 1 cut(s) 580
SfcI CTRYAG 1 cut(s) 1065
Sfr303I CCGCGG 1 cut(s) 85
SfuI TTCGAA 2 cut(s) 251, 338
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 2 cut(s) 357, 783
SmiMI CAYNNNNRTG 1 cut(s) 846
SphI GCATGC 1 cut(s) 422
Sse9I AATT 5 cut(s) 185, 242, 458, 497, 987
SseBI AGGCCT 1 cut(s) 909
SspMI CTAG 2 cut(s) 869, 894
StuI AGGCCT 1 cut(s) 909
StyD4I CCNGG 2 cut(s) 295, 666
TaaI ACNGT 2 cut(s) 353, 1066
TaiI ACGT 1 cut(s) 1019
TaqI TCGA 7 cut(s) 251, 338, 432, 482, 563, 756, 1049
TasI AATT 5 cut(s) 185, 242, 458, 497, 987
TauI GCSGC 1 cut(s) 87
TfiI GAWTC 6 cut(s) 134, 234, 313, 647, 753, 1031
Tru1I TTAA 2 cut(s) 461, 749
Tru9I TTAA 2 cut(s) 461, 749
TscAI CASTG 4 cut(s) 381, 409, 778, 1069
TseFI GTSAC 1 cut(s) 884
TseI GCWGC 4 cut(s) 259, 520, 523, 526
Tsp45I GTSAC 1 cut(s) 884
TspDTI ATGAA 2 cut(s) 777, 858
TspGWI ACGGA 2 cut(s) 214, 280
TspRI CASTG 4 cut(s) 381, 409, 778, 1069
VpaK11BI GGWCC 2 cut(s) 357, 783
XapI RAATTY 1 cut(s) 185
XbaI TCTAGA 1 cut(s) 868
XceI RCATGY 2 cut(s) 400, 422
XcmI CCANNNNNNNNNTGG 1 cut(s) 1007
XmiI GTMKAC 1 cut(s) 563
XspI CTAG 2 cut(s) 869, 894
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.