Rh2CG030400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0003259
Physical Location & Seq
Forward (+)
2275746 .. 2279231
3486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG030400.1

Sequence Viewer

Length: 1122 bp
ATGTCTTGGCTCTTCACCTCTCTCCAACCTGACGCGGAAAACGACGCGTCGTTTCTCGGCCAAACCCTCACCCGCCAACTCCGCGGCGTCGCCACCTTCCTTGCTCCGCCGCCCTCCTCCTCCAAGTCCCTTCCCTCCTCCGATTCTCCACCGCCGTCGCCGGCGCTCTTGGGAGTCCGAAACGATCTCGTGGAATTGGGCGGGAGCTTCAAGACCGGCCTCTCGCTTCTCTCCGTCGCCGGAATCTCCAAATTGGCTTCGAACTTGCTCCCGGTTCGGAACGGAGCTCCGGAAGAAGACGGCGGCGGCGGTCCCGTGCCGGGGATTACCGATGAGATTCTCGGCTTCGTCGCTCAGATTTCGAAGCTTCCGGACTGTTGGATCGGCTTTCCTATACCACTGGACCACCATGATTTTAGCATGTCCAATGCTCAGAGAGAACATGCCTCCACTGTCGAACAATTGGTTCCGGGGTTTGCTGATCTAAGAGTCAGAGTTTGCAGTTCGATGAGTGAGGAGCAATTTTGGATGATCTATTTCTTGTTGCTGCTTCCAAGACTAAATGCAGATGATTTTGAGCTTCTGTCAACCCCCAAGATTGTTGAAGCAAGAAATGTACTTCTGCACAAGCTTCAAAAAAACACGCAGGTATCCACCTCTGCGGAGTCAACTCTTCATCCAACTGAAAAGGACACCCAGGTTGAGAAGACACAAGGGGGAGAATTCTCCTCTGCAGAAAAGAAGGATCTGACAGAGATTGTAAATACAGCTGAAAGGTTAAGAATCGACGATGAAGAGAGCACTGAGCAATGGTCCGAAGCAGCGAGCATTAGTTCTGGTACTTTTGTGGATGACCCAAAAAAACTTGAACATGAAGACGATATCTCATTCAGCGATTTAGAGGATGACGAGAATGACATTTCTACTAGATTGTCAGGCCTAAGGCCAGCAAAGGACGTTAGGGAATGTTCACCCAGTGGATCAAATCATTCAAATGACTGGGTTCGGCTGAATAGAAGTTCTGCGATTGATGGTGGCCATCAAAGGACTGGTACATCGAAAGAGAAAGATTCAGAAGGTGAGGAGTCGAATGACTGGCTCACTGTAGATAAGTTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

373

Amino Acids

40.78

Weight (kDa)

4.59

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 138 - 188 5e-07 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 637
AccII CGCG 3 cut(s) 35, 47, 84
AccIII TCCGGA 2 cut(s) 289, 370
AclWI GGATC 3 cut(s) 389, 753, 988
AcoI YGGCCR 2 cut(s) 58, 1036
AcsI RAATTY 1 cut(s) 722
AcyI GRCGYC 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 977
AfaI GTAC 3 cut(s) 618, 841, 1054
AfiI CCNNNNNNNGG 2 cut(s) 320, 321
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 5 cut(s) 211, 605, 635, 869, 993
AjnI CCWGG 1 cut(s) 696
AluBI AGCT 6 cut(s) 207, 287, 367, 580, 631, 770
AluI AGCT 6 cut(s) 207, 287, 367, 580, 631, 770
Alw21I GWGCWC 2 cut(s) 289, 803
AlwI GGATC 3 cut(s) 389, 753, 988
Aor13HI TCCGGA 2 cut(s) 289, 370
AoxI GGCC 5 cut(s) 58, 217, 937, 944, 1036
ApeKI GCWGC 2 cut(s) 547, 821
ApoI RAATTY 1 cut(s) 722
AspLEI GCGC 1 cut(s) 166
AspS9I GGNCC 3 cut(s) 311, 403, 813
AsuC2I CCSGG 3 cut(s) 272, 321, 471
AsuHPI GGTGA 4 cut(s) 7, 61, 963, 1091
AsuII TTCGAA 2 cut(s) 260, 362
AvaII GGWCC 3 cut(s) 311, 403, 813
AxyI CCTNAGG 1 cut(s) 941
BaeI ACNNNNGTAYC 4 cut(s) 633, 666, 831, 864
BalI TGGCCA 1 cut(s) 1038
BanII GRGCYC 1 cut(s) 289
BauI CACGAG 1 cut(s) 188
BbsI GAAGAC 3 cut(s) 303, 713, 882
Bbv12I GWGCWC 2 cut(s) 289, 803
BbvI GCAGC 2 cut(s) 534, 833
BccI CCATC 2 cut(s) 1025, 1047
BceAI ACGGC 2 cut(s) 139, 316
BciT130I CCWGG 1 cut(s) 698
BciVI GTATCC 1 cut(s) 661
BcnI CCSGG 3 cut(s) 272, 321, 471
BfaI CTAG 1 cut(s) 927
BfmI CTRYAG 2 cut(s) 732, 1104
BfoI RGCGCY 1 cut(s) 167
BfuAI ACCTGC 1 cut(s) 637
BfuI GTATCC 1 cut(s) 661
BisI GCNGC 6 cut(s) 85, 110, 304, 307, 548, 822
BlsI GCNGC 6 cut(s) 86, 111, 305, 308, 549, 823
Bme1390I CCNGG 4 cut(s) 272, 321, 471, 698
Bme18I GGWCC 3 cut(s) 311, 403, 813
BmgT120I GGNCC 3 cut(s) 311, 403, 813
BmiI GGNNCC 2 cut(s) 313, 468
BmrFI CCNGG 4 cut(s) 272, 321, 471, 698
BmrI ACTGGG 2 cut(s) 969, 1009
BmuI ACTGGG 2 cut(s) 969, 1009
BpiI GAAGAC 3 cut(s) 303, 713, 882
Bpu14I TTCGAA 2 cut(s) 260, 362
BpuMI CCSGG 3 cut(s) 272, 321, 471
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 4 cut(s) 82, 320, 470, 696
BsaWI WCCGGW 2 cut(s) 289, 370
Bsc4I CCNNNNNNNGG 2 cut(s) 320, 321
Bse118I RCCGGY 2 cut(s) 160, 215
Bse1I ACTGG 5 cut(s) 405, 975, 1004, 1054, 1100
Bse21I CCTNAGG 1 cut(s) 941
Bse3DI GCAATG 1 cut(s) 815
BseAI TCCGGA 2 cut(s) 289, 370
BseBI CCWGG 1 cut(s) 698
BseDI CCNNGG 4 cut(s) 82, 320, 470, 696
BseGI GGATG 4 cut(s) 534, 676, 856, 910
BseLI CCNNNNNNNGG 2 cut(s) 320, 321
BseMI GCAATG 1 cut(s) 815
BseMII CTCAG 3 cut(s) 368, 446, 795
BseNI ACTGG 5 cut(s) 405, 975, 1004, 1054, 1100
BseRI GAGGAG 6 cut(s) 106, 109, 127, 530, 718, 1097
BseXI GCAGC 2 cut(s) 534, 833
BsgI GTGCAG 1 cut(s) 608
Bsh1236I CGCG 3 cut(s) 35, 47, 84
BshFI GGCC 5 cut(s) 60, 219, 939, 946, 1038
BsiHKAI GWGCWC 2 cut(s) 289, 803
BsiSI CCGG 8 cut(s) 161, 216, 240, 272, 290, 320, 371, 470
BslFI GGGAC 2 cut(s) 112, 297
BslI CCNNNNNNNGG 2 cut(s) 320, 321
BsmFI GGGAC 2 cut(s) 112, 297
BsnI GGCC 5 cut(s) 60, 219, 939, 946, 1038
Bsp119I TTCGAA 2 cut(s) 260, 362
Bsp1286I GDGCHC 2 cut(s) 289, 803
Bsp13I TCCGGA 2 cut(s) 289, 370
Bsp143I GATC 6 cut(s) 184, 381, 481, 531, 745, 980
BspANI GGCC 5 cut(s) 60, 219, 939, 946, 1038
BspCNI CTCAG 3 cut(s) 367, 445, 796
BspEI TCCGGA 2 cut(s) 289, 370
BspFNI CGCG 3 cut(s) 35, 47, 84
BspLI GGNNCC 2 cut(s) 313, 468
BspMAI CTGCAG 1 cut(s) 736
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 3 cut(s) 389, 753, 988
BspQI GCTCTTC 1 cut(s) 17
BspT104I TTCGAA 2 cut(s) 260, 362
BsrDI GCAATG 1 cut(s) 815
BsrFI RCCGGY 2 cut(s) 160, 215
BsrI ACTGG 5 cut(s) 405, 975, 1004, 1054, 1100
BssAI RCCGGY 2 cut(s) 160, 215
BssECI CCNNGG 4 cut(s) 82, 320, 470, 696
BssMI GATC 6 cut(s) 184, 381, 481, 531, 745, 980
BssNI GRCGYC 1 cut(s) 87
BssSI CACGAG 1 cut(s) 188
Bst2BI CACGAG 1 cut(s) 188
Bst2UI CCWGG 1 cut(s) 698
Bst4CI ACNGT 3 cut(s) 377, 454, 1105
Bst6I CTCTTC 3 cut(s) 17, 678, 789
BstACI GRCGYC 1 cut(s) 87
BstBI TTCGAA 2 cut(s) 260, 362
BstC8I GCNNGC 3 cut(s) 162, 826, 948
BstDEI CTNAG 5 cut(s) 354, 432, 485, 804, 941
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 4 cut(s) 534, 676, 856, 910
BstFNI CGCG 3 cut(s) 35, 47, 84
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 6 cut(s) 187, 384, 484, 534, 748, 983
BstMBI GATC 6 cut(s) 184, 381, 481, 531, 745, 980
BstMWI GCNNNNNNNGC 1 cut(s) 81
BstNI CCWGG 1 cut(s) 698
BstNSI RCATGY 2 cut(s) 424, 446
BstSCI CCNGG 4 cut(s) 270, 319, 469, 696
BstSFI CTRYAG 2 cut(s) 732, 1104
BstUI CGCG 3 cut(s) 35, 47, 84
BstV1I GCAGC 2 cut(s) 534, 833
BstV2I GAAGAC 3 cut(s) 303, 713, 882
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
Bsu36I CCTNAGG 1 cut(s) 941
BsuI GTATCC 1 cut(s) 661
BsuRI GGCC 5 cut(s) 60, 219, 939, 946, 1038
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 4 cut(s) 534, 676, 856, 910
BtsIMutI CAGTG 5 cut(s) 398, 450, 801, 982, 1101
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 3 cut(s) 162, 826, 948
CfoI GCGC 1 cut(s) 166
Cfr10I RCCGGY 2 cut(s) 160, 215
Cfr13I GGNCC 3 cut(s) 311, 403, 813
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 4 cut(s) 36, 41, 53, 76
Csp6I GTAC 3 cut(s) 617, 840, 1053
CspCI CAANNNNNGTGG 2 cut(s) 82, 117
CviAII CATG 4 cut(s) 410, 421, 443, 872
CviQI GTAC 3 cut(s) 617, 840, 1053
DdeI CTNAG 5 cut(s) 354, 432, 485, 804, 941
DpnI GATC 6 cut(s) 186, 383, 483, 533, 747, 982
DpnII GATC 6 cut(s) 184, 381, 481, 531, 745, 980
DraIII CACNNNGTG 1 cut(s) 977
EaeI YGGCCR 2 cut(s) 58, 1036
Eam1104I CTCTTC 3 cut(s) 17, 678, 789
EarI CTCTTC 3 cut(s) 17, 678, 789
EciI GGCGGA 1 cut(s) 96
Ecl136II GAGCTC 1 cut(s) 287
Eco147I AGGCCT 1 cut(s) 939
Eco24I GRGCYC 1 cut(s) 289
Eco32I GATATC 1 cut(s) 883
Eco47I GGWCC 3 cut(s) 311, 403, 813
Eco53kI GAGCTC 1 cut(s) 287
Eco81I CCTNAGG 1 cut(s) 941
EcoICRI GAGCTC 1 cut(s) 287
EcoRI GAATTC 1 cut(s) 722
EcoRII CCWGG 1 cut(s) 696
EcoRV GATATC 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 289
FaeI CATG 4 cut(s) 413, 424, 446, 875
FaiI YATR 5 cut(s) 395, 411, 422, 444, 873
FaqI GGGAC 2 cut(s) 112, 297
FatI CATG 4 cut(s) 409, 420, 442, 871
FauI CCCGC 2 cut(s) 80, 194
Fnu4HI GCNGC 6 cut(s) 85, 110, 304, 307, 548, 822
FokI GGATG 4 cut(s) 541, 663, 863, 917
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 6 cut(s) 85, 110, 304, 307, 548, 822
FspBI CTAG 1 cut(s) 927
GlaI GCGC 1 cut(s) 165
GluI GCNGC 6 cut(s) 85, 110, 304, 307, 548, 822
HaeII RGCGCY 1 cut(s) 167
HaeIII GGCC 5 cut(s) 60, 219, 939, 946, 1038
HapII CCGG 8 cut(s) 161, 216, 240, 272, 290, 320, 371, 470
HgaI GACGC 4 cut(s) 36, 41, 53, 76
HhaI GCGC 1 cut(s) 166
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 4 cut(s) 413, 424, 446, 875
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HincII GTYRAC 2 cut(s) 588, 669
HindII GTYRAC 2 cut(s) 588, 669
HindIII AAGCTT 2 cut(s) 365, 629
HinfI GANTC 9 cut(s) 143, 174, 243, 337, 489, 665, 783, 1070, 1085
HpaII CCGG 8 cut(s) 161, 216, 240, 272, 290, 320, 371, 470
HphI GGTGA 4 cut(s) 7, 61, 963, 1091
Hpy166II GTNNAC 3 cut(s) 588, 669, 971
Hpy188I TCNGA 9 cut(s) 142, 179, 279, 357, 435, 494, 750, 817, 1075
Hpy188III TCNNGA 3 cut(s) 211, 290, 371
Hpy8I GTNNAC 3 cut(s) 588, 669, 971
Hpy99I CGWCG 7 cut(s) 47, 52, 92, 160, 239, 353, 791
HpyAV CCTTC 4 cut(s) 106, 140, 736, 1070
HpyCH4III ACNGT 3 cut(s) 377, 454, 1105
HpyCH4IV ACGT 1 cut(s) 957
HpyCH4V TGCA 4 cut(s) 501, 566, 625, 734
HpyF10VI GCNNNNNNNGC 1 cut(s) 81
HpyF3I CTNAG 5 cut(s) 354, 432, 485, 804, 941
HpySE526I ACGT 1 cut(s) 957
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 4 cut(s) 413, 424, 446, 875
HspAI GCGC 1 cut(s) 164
Kpn2I TCCGGA 2 cut(s) 289, 370
KroI GCCGGC 1 cut(s) 160
KroNI GCCGGC 1 cut(s) 162
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 6 cut(s) 184, 381, 481, 531, 745, 980
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 6 cut(s) 109, 204, 273, 284, 292, 517
Lsp1109I GCAGC 2 cut(s) 534, 833
MaeI CTAG 1 cut(s) 927
MaeII ACGT 1 cut(s) 957
MalI GATC 6 cut(s) 186, 383, 483, 533, 747, 982
MboI GATC 6 cut(s) 184, 381, 481, 531, 745, 980
MboII GAAGA 7 cut(s) 4, 305, 308, 665, 718, 806, 887
MfeI CAATTG 1 cut(s) 461
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 2 cut(s) 289, 803
MlsI TGGCCA 1 cut(s) 1038
MluCI AATT 5 cut(s) 194, 251, 461, 521, 722
MluI ACGCGT 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 1038
MlyI GAGTC 4 cut(s) 183, 498, 674, 1094
MmeI TCCRAC 3 cut(s) 49, 359, 704
Mox20I TGGCCA 1 cut(s) 1038
MreI CGCCGGCG 1 cut(s) 160
MroI TCCGGA 2 cut(s) 289, 370
MroNI GCCGGC 1 cut(s) 160
MscI TGGCCA 1 cut(s) 1038
MseI TTAA 1 cut(s) 779
MslI CAYNNNNRTG 1 cut(s) 993
Msp20I TGGCCA 1 cut(s) 1038
MspA1I CMGCKG 2 cut(s) 84, 770
MspI CCGG 8 cut(s) 161, 216, 240, 272, 290, 320, 371, 470
MspR9I CCNGG 4 cut(s) 272, 321, 471, 698
MunI CAATTG 1 cut(s) 461
MvaI CCWGG 1 cut(s) 698
MvnI CGCG 3 cut(s) 35, 47, 84
MwoI GCNNNNNNNGC 1 cut(s) 81
NaeI GCCGGC 1 cut(s) 162
NciI CCSGG 3 cut(s) 272, 321, 471
NdeII GATC 6 cut(s) 184, 381, 481, 531, 745, 980
NgoMIV GCCGGC 1 cut(s) 160
NlaIII CATG 4 cut(s) 413, 424, 446, 875
NlaIV GGNNCC 2 cut(s) 313, 468
NmeAIII GCCGAG 2 cut(s) 36, 321
NspI RCATGY 2 cut(s) 424, 446
NspV TTCGAA 2 cut(s) 260, 362
PceI AGGCCT 1 cut(s) 939
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PdiI GCCGGC 1 cut(s) 162
PfeI GAWTC 5 cut(s) 143, 243, 337, 783, 1070
PkrI GCNGC 6 cut(s) 86, 111, 305, 308, 549, 823
PleI GAGTC 4 cut(s) 182, 497, 673, 1093
PpsI GAGTC 4 cut(s) 182, 497, 673, 1093
Psp124BI GAGCTC 1 cut(s) 289
Psp6I CCWGG 1 cut(s) 696
PspGI CCWGG 1 cut(s) 696
PspN4I GGNNCC 2 cut(s) 313, 468
PspPI GGNCC 3 cut(s) 311, 403, 813
PstI CTGCAG 1 cut(s) 736
PsuI RGATCY 1 cut(s) 745
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 3 cut(s) 618, 841, 1054
RsaNI GTAC 3 cut(s) 617, 840, 1053
RseI CAYNNNNRTG 1 cut(s) 993
SacI GAGCTC 1 cut(s) 289
SacII CCGCGG 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 779
SatI GCNGC 6 cut(s) 85, 110, 304, 307, 548, 822
Sau3AI GATC 6 cut(s) 184, 381, 481, 531, 745, 980
Sau96I GGNCC 3 cut(s) 311, 403, 813
SchI GAGTC 4 cut(s) 183, 498, 674, 1094
ScrFI CCNGG 4 cut(s) 272, 321, 471, 698
SduI GDGCHC 2 cut(s) 289, 803
SfcI CTRYAG 2 cut(s) 732, 1104
Sfr303I CCGCGG 1 cut(s) 85
SfuI TTCGAA 2 cut(s) 260, 362
SgrAI CRCCGGYG 1 cut(s) 160
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 3 cut(s) 311, 403, 813
SmiMI CAYNNNNRTG 1 cut(s) 993
Sse9I AATT 5 cut(s) 194, 251, 461, 521, 722
SseBI AGGCCT 1 cut(s) 939
SspMI CTAG 1 cut(s) 927
SstI GAGCTC 1 cut(s) 289
StuI AGGCCT 1 cut(s) 939
StyD4I CCNGG 4 cut(s) 270, 319, 469, 696
TaaI ACNGT 3 cut(s) 377, 454, 1105
TaiI ACGT 1 cut(s) 960
TaqI TCGA 7 cut(s) 260, 362, 456, 506, 786, 1058, 1088
TasI AATT 5 cut(s) 194, 251, 461, 521, 722
TatI WGTACW 1 cut(s) 616
TauI GCSGC 4 cut(s) 87, 112, 306, 309
TfiI GAWTC 5 cut(s) 143, 243, 337, 783, 1070
Tru1I TTAA 1 cut(s) 779
Tru9I TTAA 1 cut(s) 779
TscAI CASTG 5 cut(s) 405, 457, 808, 982, 1108
TseI GCWGC 2 cut(s) 547, 821
TspDTI ATGAA 3 cut(s) 665, 807, 888
TspGWI ACGGA 2 cut(s) 223, 297
TspRI CASTG 5 cut(s) 405, 457, 808, 982, 1108
VpaK11BI GGWCC 3 cut(s) 311, 403, 813
XapI RAATTY 1 cut(s) 722
XceI RCATGY 2 cut(s) 424, 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 1046
XspI CTAG 1 cut(s) 927
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.