Rroxscaffold_2G00153450

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003259
Physical Location & Seq
Reverse (-)
90256621 .. 90258643
2023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153450.1

Sequence Viewer

Length: 915 bp
ATGTCTTGGCTCTTCACCTCTCTCCAACCCGACGCGGAAAACGACGCGTCGTTTCTCGGCCAAACCCTCACCCGCCAACTCCGCGGCGTCGCCACCTTCCTCGCTCCGCCGCCCTCCTCCTCCAAGTCCCTTCCCTCCTCCGATTCTCCACCGCCGTCGCCGTCGCCGTCGCCGTCGCCGGCGCTCTTGGGAGTCCGAAACGATCTCGCGGAATTGGGCGGGAGCTTCAAGACCGGCCTCTCGCTTCTCTCCGTCGCCGGAATCTCCAAATTGGCTTCGAACTTGCTCCCGGTTCGGAACGGAGCTCCGGAAGACGACGGCGGCGGTCCCGTGCCGGGGATTACCGATGAGATTCTCGGCTTCGTCGCTCAGATTTCGAAGCTTCCGGACTGTTGGACCGGCTTTCCTATACCAGTGGACCACCATGATTTTAGCATGTCCAATGCTCAGAGAGAACATGCCTCCGCTGTCGAACAATTGGTTCCGGGGTTTGGGGATCTAAGAGTCAGAGTTTGCAGTTCGATGAGCGAGGAGCAATTTTGGGTGATCTATTTCTTGTTGCTGCTTCCAAGACTAAATGCAGATGATTTTGAGCTTCTGTCAACCCCCAAGATTGTTGAAGCAAGAAATGTACTTCTGCACAAGCTTCAAAAGAACACGCAGGTATCCACCTCTGAGGAGTCAACTCTTCATTCAACTGAAAAGGACACCCAGGTTGACAAGACACAAGGGGGAGAATTCTCGTCTGCAGAAAAGAAGGATCTGACAGAGATTGTAAATACAGCTGAAAGGTTAAGAATCGACGATGAAGAGAGCACTGAGCAATGGTCCGAAGCAGCGAGTATTAGTTCTGGTACTTTTGTGGATGACCCAAAAAACTTGAACATGAAGACGATATCTCATTCAGCGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

304

Amino Acids

32.83

Weight (kDa)

4.7

Isoelectric Point (pI)

52.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 143 - 193 4.2e-06 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 652
AccII CGCG 4 cut(s) 35, 47, 84, 209
AccIII TCCGGA 2 cut(s) 307, 385
AclWI GGATC 2 cut(s) 504, 768
AcoI YGGCCR 1 cut(s) 58
AcsI RAATTY 1 cut(s) 737
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 2 cut(s) 633, 856
AfiI CCNNNNNNNGG 3 cut(s) 335, 336, 491
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 5 cut(s) 229, 620, 650, 696, 883
AjnI CCWGG 1 cut(s) 711
AluBI AGCT 6 cut(s) 225, 305, 382, 595, 646, 785
AluI AGCT 6 cut(s) 225, 305, 382, 595, 646, 785
Alw21I GWGCWC 2 cut(s) 307, 818
AlwI GGATC 2 cut(s) 504, 768
Aor13HI TCCGGA 2 cut(s) 307, 385
AoxI GGCC 2 cut(s) 58, 235
ApeKI GCWGC 2 cut(s) 562, 836
ApoI RAATTY 1 cut(s) 737
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 4 cut(s) 326, 396, 418, 828
AsuC2I CCSGG 3 cut(s) 290, 336, 486
AsuHPI GGTGA 3 cut(s) 7, 61, 556
AsuII TTCGAA 2 cut(s) 278, 377
AvaII GGWCC 4 cut(s) 326, 396, 418, 828
BaeI ACNNNNGTAYC 4 cut(s) 648, 681, 846, 879
BanII GRGCYC 1 cut(s) 307
BbsI GAAGAC 2 cut(s) 318, 896
Bbv12I GWGCWC 2 cut(s) 307, 818
BbvI GCAGC 2 cut(s) 549, 848
BceAI ACGGC 5 cut(s) 139, 145, 151, 157, 334
BciT130I CCWGG 1 cut(s) 713
BciVI GTATCC 1 cut(s) 676
BcnI CCSGG 3 cut(s) 290, 336, 486
BfmI CTRYAG 1 cut(s) 747
BfoI RGCGCY 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 652
BfuI GTATCC 1 cut(s) 676
BisI GCNGC 5 cut(s) 85, 110, 322, 563, 837
BlsI GCNGC 5 cut(s) 86, 111, 323, 564, 838
Bme1390I CCNGG 4 cut(s) 290, 336, 486, 713
Bme18I GGWCC 4 cut(s) 326, 396, 418, 828
BmgT120I GGNCC 4 cut(s) 326, 396, 418, 828
BmiI GGNNCC 2 cut(s) 328, 483
BmrFI CCNGG 4 cut(s) 290, 336, 486, 713
BpiI GAAGAC 2 cut(s) 318, 896
Bpu14I TTCGAA 2 cut(s) 278, 377
BpuMI CCSGG 3 cut(s) 290, 336, 486
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 4 cut(s) 82, 335, 485, 711
BsaWI WCCGGW 2 cut(s) 307, 385
Bsc4I CCNNNNNNNGG 3 cut(s) 335, 336, 491
Bse118I RCCGGY 3 cut(s) 178, 233, 398
Bse1I ACTGG 1 cut(s) 413
Bse3DI GCAATG 1 cut(s) 830
BseAI TCCGGA 2 cut(s) 307, 385
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 4 cut(s) 82, 335, 485, 711
BseGI GGATG 1 cut(s) 871
BseLI CCNNNNNNNGG 3 cut(s) 335, 336, 491
BseMI GCAATG 1 cut(s) 830
BseMII CTCAG 4 cut(s) 383, 461, 666, 810
BseNI ACTGG 1 cut(s) 413
BseRI GAGGAG 5 cut(s) 106, 109, 127, 545, 692
BseXI GCAGC 2 cut(s) 549, 848
BsgI GTGCAG 1 cut(s) 623
Bsh1236I CGCG 4 cut(s) 35, 47, 84, 209
BshFI GGCC 2 cut(s) 60, 237
BsiHKAI GWGCWC 2 cut(s) 307, 818
BsiSI CCGG 9 cut(s) 179, 234, 258, 290, 308, 335, 386, 399, 485
BslFI GGGAC 2 cut(s) 112, 312
BslI CCNNNNNNNGG 3 cut(s) 335, 336, 491
BsmFI GGGAC 2 cut(s) 112, 312
BsnI GGCC 2 cut(s) 60, 237
Bsp119I TTCGAA 2 cut(s) 278, 377
Bsp1286I GDGCHC 2 cut(s) 307, 818
Bsp13I TCCGGA 2 cut(s) 307, 385
Bsp143I GATC 4 cut(s) 202, 496, 546, 760
BspANI GGCC 2 cut(s) 60, 237
BspCNI CTCAG 4 cut(s) 382, 460, 667, 811
BspEI TCCGGA 2 cut(s) 307, 385
BspFNI CGCG 4 cut(s) 35, 47, 84, 209
BspLI GGNNCC 2 cut(s) 328, 483
BspMAI CTGCAG 1 cut(s) 751
BspMI ACCTGC 1 cut(s) 652
BspPI GGATC 2 cut(s) 504, 768
BspQI GCTCTTC 1 cut(s) 17
BspT104I TTCGAA 2 cut(s) 278, 377
BsrDI GCAATG 1 cut(s) 830
BsrFI RCCGGY 3 cut(s) 178, 233, 398
BsrI ACTGG 1 cut(s) 413
BssAI RCCGGY 3 cut(s) 178, 233, 398
BssECI CCNNGG 4 cut(s) 82, 335, 485, 711
BssMI GATC 4 cut(s) 202, 496, 546, 760
BssNI GRCGYC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 1 cut(s) 392
Bst6I CTCTTC 3 cut(s) 17, 693, 804
BstACI GRCGYC 1 cut(s) 87
BstBI TTCGAA 2 cut(s) 278, 377
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 5 cut(s) 369, 447, 500, 675, 819
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 1 cut(s) 871
BstFNI CGCG 4 cut(s) 35, 47, 84, 209
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 4 cut(s) 205, 499, 549, 763
BstMBI GATC 4 cut(s) 202, 496, 546, 760
BstMWI GCNNNNNNNGC 1 cut(s) 81
BstNI CCWGG 1 cut(s) 713
BstNSI RCATGY 2 cut(s) 439, 461
BstSCI CCNGG 4 cut(s) 288, 334, 484, 711
BstSFI CTRYAG 1 cut(s) 747
BstUI CGCG 4 cut(s) 35, 47, 84, 209
BstV1I GCAGC 2 cut(s) 549, 848
BstV2I GAAGAC 2 cut(s) 318, 896
BstX2I RGATCY 2 cut(s) 496, 760
BstYI RGATCY 2 cut(s) 496, 760
BsuI GTATCC 1 cut(s) 676
BsuRI GGCC 2 cut(s) 60, 237
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 1 cut(s) 871
BtsIMutI CAGTG 2 cut(s) 420, 816
BveI ACCTGC 1 cut(s) 652
Cac8I GCNNGC 1 cut(s) 180
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 3 cut(s) 178, 233, 398
Cfr13I GGNCC 4 cut(s) 326, 396, 418, 828
Cfr42I CCGCGG 1 cut(s) 85
CseI GACGC 4 cut(s) 36, 41, 53, 76
Csp6I GTAC 2 cut(s) 632, 855
CviAII CATG 4 cut(s) 425, 436, 458, 886
CviQI GTAC 2 cut(s) 632, 855
DdeI CTNAG 5 cut(s) 369, 447, 500, 675, 819
DpnI GATC 4 cut(s) 204, 498, 548, 762
DpnII GATC 4 cut(s) 202, 496, 546, 760
EaeI YGGCCR 1 cut(s) 58
Eam1104I CTCTTC 3 cut(s) 17, 693, 804
EarI CTCTTC 3 cut(s) 17, 693, 804
EciI GGCGGA 1 cut(s) 96
Ecl136II GAGCTC 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 307
Eco32I GATATC 1 cut(s) 897
Eco47I GGWCC 4 cut(s) 326, 396, 418, 828
Eco53kI GAGCTC 1 cut(s) 305
EcoICRI GAGCTC 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 737
EcoRII CCWGG 1 cut(s) 711
EcoRV GATATC 1 cut(s) 897
EcoT38I GRGCYC 1 cut(s) 307
FaeI CATG 4 cut(s) 428, 439, 461, 889
FaiI YATR 5 cut(s) 410, 426, 437, 459, 887
FaqI GGGAC 2 cut(s) 112, 312
FatI CATG 4 cut(s) 424, 435, 457, 885
FauI CCCGC 2 cut(s) 80, 212
Fnu4HI GCNGC 5 cut(s) 85, 110, 322, 563, 837
FokI GGATG 1 cut(s) 878
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 5 cut(s) 85, 110, 322, 563, 837
GlaI GCGC 1 cut(s) 183
GluI GCNGC 5 cut(s) 85, 110, 322, 563, 837
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 2 cut(s) 60, 237
HapII CCGG 9 cut(s) 179, 234, 258, 290, 308, 335, 386, 399, 485
HgaI GACGC 4 cut(s) 36, 41, 53, 76
HhaI GCGC 1 cut(s) 184
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 4 cut(s) 428, 439, 461, 889
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HincII GTYRAC 3 cut(s) 603, 684, 718
HindII GTYRAC 3 cut(s) 603, 684, 718
HindIII AAGCTT 2 cut(s) 380, 644
HinfI GANTC 7 cut(s) 143, 192, 261, 352, 504, 680, 798
HpaII CCGG 9 cut(s) 179, 234, 258, 290, 308, 335, 386, 399, 485
HphI GGTGA 3 cut(s) 7, 61, 556
Hpy166II GTNNAC 4 cut(s) 418, 603, 684, 718
Hpy188I TCNGA 9 cut(s) 142, 197, 297, 372, 450, 509, 676, 765, 832
Hpy188III TCNNGA 3 cut(s) 229, 308, 386
Hpy8I GTNNAC 4 cut(s) 418, 603, 684, 718
HpyAV CCTTC 3 cut(s) 106, 140, 751
HpyCH4III ACNGT 1 cut(s) 392
HpyCH4V TGCA 4 cut(s) 516, 581, 640, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 81
HpyF3I CTNAG 5 cut(s) 369, 447, 500, 675, 819
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 4 cut(s) 428, 439, 461, 889
HspAI GCGC 1 cut(s) 182
Kpn2I TCCGGA 2 cut(s) 307, 385
KroI GCCGGC 1 cut(s) 178
KroNI GCCGGC 1 cut(s) 180
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 4 cut(s) 202, 496, 546, 760
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 6 cut(s) 109, 222, 291, 302, 310, 532
Lsp1109I GCAGC 2 cut(s) 549, 848
MalI GATC 4 cut(s) 204, 498, 548, 762
MboI GATC 4 cut(s) 202, 496, 546, 760
MboII GAAGA 5 cut(s) 4, 323, 680, 821, 901
MfeI CAATTG 1 cut(s) 476
MflI RGATCY 2 cut(s) 496, 760
MhlI GDGCHC 2 cut(s) 307, 818
MluCI AATT 5 cut(s) 212, 269, 476, 536, 737
MluI ACGCGT 1 cut(s) 45
MlyI GAGTC 3 cut(s) 201, 513, 689
MmeI TCCRAC 2 cut(s) 49, 374
MreI CGCCGGCG 1 cut(s) 178
MroI TCCGGA 2 cut(s) 307, 385
MroNI GCCGGC 1 cut(s) 178
MseI TTAA 1 cut(s) 794
MspA1I CMGCKG 3 cut(s) 84, 467, 785
MspI CCGG 9 cut(s) 179, 234, 258, 290, 308, 335, 386, 399, 485
MspR9I CCNGG 4 cut(s) 290, 336, 486, 713
MunI CAATTG 1 cut(s) 476
MvaI CCWGG 1 cut(s) 713
MvnI CGCG 4 cut(s) 35, 47, 84, 209
MwoI GCNNNNNNNGC 1 cut(s) 81
NaeI GCCGGC 1 cut(s) 180
NciI CCSGG 3 cut(s) 290, 336, 486
NdeII GATC 4 cut(s) 202, 496, 546, 760
NgoMIV GCCGGC 1 cut(s) 178
NlaIII CATG 4 cut(s) 428, 439, 461, 889
NlaIV GGNNCC 2 cut(s) 328, 483
NmeAIII GCCGAG 2 cut(s) 36, 336
NspI RCATGY 2 cut(s) 439, 461
NspV TTCGAA 2 cut(s) 278, 377
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 3 cut(s) 39, 164, 170
PdiI GCCGGC 1 cut(s) 180
PfeI GAWTC 4 cut(s) 143, 261, 352, 798
PkrI GCNGC 5 cut(s) 86, 111, 323, 564, 838
PleI GAGTC 3 cut(s) 200, 512, 688
PpsI GAGTC 3 cut(s) 200, 512, 688
Psp124BI GAGCTC 1 cut(s) 307
Psp6I CCWGG 1 cut(s) 711
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 2 cut(s) 328, 483
PspPI GGNCC 4 cut(s) 326, 396, 418, 828
PstI CTGCAG 1 cut(s) 751
PsuI RGATCY 2 cut(s) 496, 760
PvuII CAGCTG 1 cut(s) 785
RsaI GTAC 2 cut(s) 633, 856
RsaNI GTAC 2 cut(s) 632, 855
SacI GAGCTC 1 cut(s) 307
SacII CCGCGG 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 794
SatI GCNGC 5 cut(s) 85, 110, 322, 563, 837
Sau3AI GATC 4 cut(s) 202, 496, 546, 760
Sau96I GGNCC 4 cut(s) 326, 396, 418, 828
SchI GAGTC 3 cut(s) 201, 513, 689
ScrFI CCNGG 4 cut(s) 290, 336, 486, 713
SduI GDGCHC 2 cut(s) 307, 818
SfcI CTRYAG 1 cut(s) 747
Sfr303I CCGCGG 1 cut(s) 85
SfuI TTCGAA 2 cut(s) 278, 377
SgrAI CRCCGGYG 1 cut(s) 178
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 4 cut(s) 326, 396, 418, 828
Sse9I AATT 5 cut(s) 212, 269, 476, 536, 737
SstI GAGCTC 1 cut(s) 307
StyD4I CCNGG 4 cut(s) 288, 334, 484, 711
TaaI ACNGT 1 cut(s) 392
TaqI TCGA 5 cut(s) 278, 377, 471, 521, 801
TasI AATT 5 cut(s) 212, 269, 476, 536, 737
TatI WGTACW 1 cut(s) 631
TauI GCSGC 3 cut(s) 87, 112, 324
TfiI GAWTC 4 cut(s) 143, 261, 352, 798
Tru1I TTAA 1 cut(s) 794
Tru9I TTAA 1 cut(s) 794
TscAI CASTG 2 cut(s) 420, 823
TseI GCWGC 2 cut(s) 562, 836
TspDTI ATGAA 3 cut(s) 680, 822, 902
TspGWI ACGGA 2 cut(s) 241, 315
TspRI CASTG 2 cut(s) 420, 823
VpaK11BI GGWCC 4 cut(s) 326, 396, 418, 828
XapI RAATTY 1 cut(s) 737
XceI RCATGY 2 cut(s) 439, 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.