pycom02g01980

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0003259
Physical Location & Seq
Forward (+)
1203075 .. 1204117
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01980.2

Sequence Viewer

Length: 666 bp
ATGTCTTGGCTCTTCAGCTCTCCCCGCCAACCAGATGACGACGACGACCCGCGTGATGTAGAACCCAAGCAACCCCAAAACGACCTGTCGTTTCTCCGCCAAACCCTCGGCCGCCAACTCCGCGGCGTAGCCACCTTCCTCGCTCCACCTCCATCACCGCCATCCAACGACGCCCTTCCCTCGTCCAATTCCCAGCCCCAGTCGCAATCGCAATCTCAATCGCCGGCGCTTTTGGGAATCCGGAATGATCTGGTGGAGATCGGCGGGAGCTTCAAGACCGGCCTCTCGCTGCTCTCTACTAACAGCAACAAAGCCGTCGCTGAGATCTCCAAGTTCGCTTCGGGCTTGTTGCAGCTTCAGAACGAAGCTTCAGAAGAAGGTGGAGCTGATGAAGACGGCGATGATGGCGGCGGTCACGTGCCGGGGATCACGGACGAGGTTCTCGGTTTCGTCACCGAGATTTCGGTGGTGCCGGACTGCTGGACCGACTTTCCAATGACGCTCGAACATGATTTCAGCATGTCGGATGCTCAGAGAGAGCATGTGTCCGCTGTTTTACAATTGGTTACGGGATTTGCAGATCTAAGAGCCCGGCTTTGCAATTCCATGAGTGAGGAGCAATTTTGGATCGATCTATTTCTTGTTGTTGCTCCCGAGACTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

222

Amino Acids

23.95

Weight (kDa)

4.22

Isoelectric Point (pI)

69.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 469
AccII CGCG 2 cut(s) 52, 123
AccIII TCCGGA 1 cut(s) 240
AclWI GGATC 2 cut(s) 434, 635
AcoI YGGCCR 1 cut(s) 109
AcuI CTGAAG 2 cut(s) 341, 354
AcvI CACGTG 1 cut(s) 418
AcyI GRCGYC 1 cut(s) 171
AgsI TTSAA 1 cut(s) 274
AluBI AGCT 5 cut(s) 18, 270, 355, 368, 386
AluI AGCT 5 cut(s) 18, 270, 355, 368, 386
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 2 cut(s) 434, 635
Ama87I CYCGRG 1 cut(s) 653
Aor13HI TCCGGA 1 cut(s) 240
AoxI GGCC 2 cut(s) 109, 280
ApeKI GCWGC 2 cut(s) 289, 352
AspLEI GCGC 1 cut(s) 229
AspS9I GGNCC 1 cut(s) 483
AsuC2I CCSGG 2 cut(s) 423, 592
AsuHPI GGTGA 2 cut(s) 147, 445
AvaI CYCGRG 1 cut(s) 653
AvaII GGWCC 1 cut(s) 483
BanI GGYRCC 1 cut(s) 469
BanII GRGCYC 1 cut(s) 592
BbrPI CACGTG 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 399
BbvI GCAGC 2 cut(s) 276, 364
BccI CCATC 3 cut(s) 160, 169, 398
BceAI ACGGC 2 cut(s) 299, 412
BcnI CCSGG 2 cut(s) 423, 592
BcoDI GTCTC 1 cut(s) 650
BfoI RGCGCY 1 cut(s) 230
BglII AGATCT 2 cut(s) 324, 580
BisI GCNGC 5 cut(s) 112, 124, 290, 353, 409
BlsI GCNGC 5 cut(s) 113, 125, 291, 354, 410
Bme1390I CCNGG 2 cut(s) 423, 592
Bme18I GGWCC 1 cut(s) 483
BmeT110I CYCGRG 1 cut(s) 653
BmgT120I GGNCC 1 cut(s) 483
BmiI GGNNCC 1 cut(s) 471
BmrFI CCNGG 2 cut(s) 423, 592
BmrI ACTGGG 1 cut(s) 193
BmsI GCATC 1 cut(s) 517
BmuI ACTGGG 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 399
BpuMI CCSGG 2 cut(s) 423, 592
Bsa29I ATCGAT 1 cut(s) 630
BsaAI YACGTR 1 cut(s) 418
BsaHI GRCGYC 1 cut(s) 171
BsaJI CCNNGG 3 cut(s) 106, 121, 422
BsaWI WCCGGW 1 cut(s) 240
Bse118I RCCGGY 2 cut(s) 223, 278
Bse1I ACTGG 1 cut(s) 199
BseAI TCCGGA 1 cut(s) 240
BseCI ATCGAT 1 cut(s) 630
BseDI CCNNGG 3 cut(s) 106, 121, 422
BseGI GGATG 2 cut(s) 161, 532
BseMII CTCAG 2 cut(s) 312, 545
BseNI ACTGG 1 cut(s) 199
BseRI GAGGAG 1 cut(s) 629
BseX3I CGGCCG 1 cut(s) 109
BseXI GCAGC 2 cut(s) 276, 364
BseYI CCCAGC 1 cut(s) 192
Bsh1236I CGCG 2 cut(s) 52, 123
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 2 cut(s) 111, 282
BshNI GGYRCC 1 cut(s) 469
BshVI ATCGAT 1 cut(s) 630
BsiEI CGRYCG 1 cut(s) 112
BsiHKCI CYCGRG 1 cut(s) 653
BsiSI CCGG 6 cut(s) 224, 241, 279, 422, 473, 592
BsmAI GTCTC 1 cut(s) 650
BsnI GGCC 2 cut(s) 111, 282
BsoBI CYCGRG 1 cut(s) 653
Bsp1286I GDGCHC 1 cut(s) 592
Bsp13I TCCGGA 1 cut(s) 240
Bsp143I GATC 7 cut(s) 247, 258, 324, 426, 580, 627, 631
BspANI GGCC 2 cut(s) 111, 282
BspCNI CTCAG 2 cut(s) 313, 544
BspDI ATCGAT 1 cut(s) 630
BspEI TCCGGA 1 cut(s) 240
BspFNI CGCG 2 cut(s) 52, 123
BspLI GGNNCC 1 cut(s) 471
BspPI GGATC 2 cut(s) 434, 635
BspQI GCTCTTC 1 cut(s) 17
BspT107I GGYRCC 1 cut(s) 469
BsrFI RCCGGY 2 cut(s) 223, 278
BsrI ACTGG 1 cut(s) 199
BssAI RCCGGY 2 cut(s) 223, 278
BssECI CCNNGG 3 cut(s) 106, 121, 422
BssMI GATC 7 cut(s) 247, 258, 324, 426, 580, 627, 631
BssNI GRCGYC 1 cut(s) 171
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 171
BstBAI YACGTR 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 225
BstDEI CTNAG 3 cut(s) 321, 531, 584
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 2 cut(s) 161, 532
BstFNI CGCG 2 cut(s) 52, 123
BstH2I RGCGCY 1 cut(s) 230
BstHHI GCGC 1 cut(s) 229
BstKTI GATC 7 cut(s) 250, 261, 327, 429, 583, 630, 634
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 7 cut(s) 247, 258, 324, 426, 580, 627, 631
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 4 cut(s) 24, 120, 202, 405
BstNSI RCATGY 2 cut(s) 523, 545
BstSCI CCNGG 2 cut(s) 421, 590
BstUI CGCG 2 cut(s) 52, 123
BstV1I GCAGC 2 cut(s) 276, 364
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 2 cut(s) 324, 580
BstYI RGATCY 2 cut(s) 324, 580
BstZI CGGCCG 1 cut(s) 109
Bsu15I ATCGAT 1 cut(s) 630
BsuRI GGCC 2 cut(s) 111, 282
BsuTUI ATCGAT 1 cut(s) 630
BtgI CCRYGG 1 cut(s) 121
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 2 cut(s) 161, 532
Cac8I GCNNGC 1 cut(s) 225
CfoI GCGC 1 cut(s) 229
Cfr10I RCCGGY 2 cut(s) 223, 278
Cfr13I GGNCC 1 cut(s) 483
Cfr42I CCGCGG 1 cut(s) 124
ClaI ATCGAT 1 cut(s) 630
CseI GACGC 2 cut(s) 179, 508
CviAII CATG 4 cut(s) 509, 520, 542, 607
DdeI CTNAG 3 cut(s) 321, 531, 584
DpnI GATC 7 cut(s) 249, 260, 326, 428, 582, 629, 633
DpnII GATC 7 cut(s) 247, 258, 324, 426, 580, 627, 631
EaeI YGGCCR 1 cut(s) 109
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
EciI GGCGGA 1 cut(s) 86
EclXI CGGCCG 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 483
Eco52I CGGCCG 1 cut(s) 109
Eco57I CTGAAG 2 cut(s) 341, 354
Eco72I CACGTG 1 cut(s) 418
Eco88I CYCGRG 1 cut(s) 653
EcoT38I GRGCYC 1 cut(s) 592
FaeI CATG 4 cut(s) 512, 523, 545, 610
FaiI YATR 4 cut(s) 510, 521, 543, 608
FatI CATG 4 cut(s) 508, 519, 541, 606
FauI CCCGC 3 cut(s) 32, 57, 257
Fnu4HI GCNGC 5 cut(s) 112, 124, 290, 353, 409
FokI GGATG 2 cut(s) 148, 539
FriOI GRGCYC 1 cut(s) 592
Fsp4HI GCNGC 5 cut(s) 112, 124, 290, 353, 409
GlaI GCGC 1 cut(s) 228
GluI GCNGC 5 cut(s) 112, 124, 290, 353, 409
GsaI CCCAGC 1 cut(s) 196
HaeII RGCGCY 1 cut(s) 230
HaeIII GGCC 2 cut(s) 111, 282
HapII CCGG 6 cut(s) 224, 241, 279, 422, 473, 592
HgaI GACGC 2 cut(s) 179, 508
HhaI GCGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 4 cut(s) 512, 523, 545, 610
Hin6I GCGC 1 cut(s) 227
HinP1I GCGC 1 cut(s) 227
HindIII AAGCTT 1 cut(s) 366
HinfI GANTC 1 cut(s) 237
HpaII CCGG 6 cut(s) 224, 241, 279, 422, 473, 592
HphI GGTGA 2 cut(s) 147, 445
Hpy188I TCNGA 4 cut(s) 360, 373, 526, 534
Hpy188III TCNNGA 3 cut(s) 241, 274, 653
Hpy99I CGWCG 4 cut(s) 44, 47, 173, 320
HpyAV CCTTC 3 cut(s) 145, 185, 371
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 3 cut(s) 352, 578, 600
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 120, 202, 405
HpyF3I CTNAG 3 cut(s) 321, 531, 584
HpySE526I ACGT 1 cut(s) 417
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 4 cut(s) 512, 523, 545, 610
HspAI GCGC 1 cut(s) 227
Kpn2I TCCGGA 1 cut(s) 240
KroI GCCGGC 1 cut(s) 223
KroNI GCCGGC 1 cut(s) 225
KspI CCGCGG 1 cut(s) 124
Kzo9I GATC 7 cut(s) 247, 258, 324, 426, 580, 627, 631
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 5 cut(s) 148, 267, 383, 616, 655
Lsp1109I GCAGC 2 cut(s) 276, 364
LweI GCATC 1 cut(s) 517
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 3 cut(s) 413, 451, 565
MalI GATC 7 cut(s) 249, 260, 326, 428, 582, 629, 633
MboI GATC 7 cut(s) 247, 258, 324, 426, 580, 627, 631
MboII GAAGA 3 cut(s) 4, 386, 404
MfeI CAATTG 1 cut(s) 560
MflI RGATCY 2 cut(s) 324, 580
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 4 cut(s) 187, 560, 601, 620
MmeI TCCRAC 2 cut(s) 189, 504
MnlI CCTC 7 cut(s) 116, 149, 159, 190, 293, 430, 607
MreI CGCCGGCG 1 cut(s) 223
MroI TCCGGA 1 cut(s) 240
MroNI GCCGGC 1 cut(s) 223
MspA1I CMGCKG 2 cut(s) 123, 551
MspI CCGG 6 cut(s) 224, 241, 279, 422, 473, 592
MspR9I CCNGG 2 cut(s) 423, 592
MunI CAATTG 1 cut(s) 560
MvnI CGCG 2 cut(s) 52, 123
MwoI GCNNNNNNNGC 4 cut(s) 24, 120, 202, 405
NaeI GCCGGC 1 cut(s) 225
NciI CCSGG 2 cut(s) 423, 592
NdeII GATC 7 cut(s) 247, 258, 324, 426, 580, 627, 631
NgoMIV GCCGGC 1 cut(s) 223
NlaIII CATG 4 cut(s) 512, 523, 545, 610
NlaIV GGNNCC 1 cut(s) 471
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 413, 451
NspI RCATGY 2 cut(s) 523, 545
PciSI GCTCTTC 1 cut(s) 17
PdiI GCCGGC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 237
PkrI GCNGC 5 cut(s) 113, 125, 291, 354, 410
PmaCI CACGTG 1 cut(s) 418
PmlI CACGTG 1 cut(s) 418
Ppu21I YACGTR 1 cut(s) 418
PspCI CACGTG 1 cut(s) 418
PspFI CCCAGC 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 471
PspPI GGNCC 1 cut(s) 483
PsuI RGATCY 2 cut(s) 324, 580
SacII CCGCGG 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 17
SatI GCNGC 5 cut(s) 112, 124, 290, 353, 409
Sau3AI GATC 7 cut(s) 247, 258, 324, 426, 580, 627, 631
Sau96I GGNCC 1 cut(s) 483
ScrFI CCNGG 2 cut(s) 423, 592
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 1 cut(s) 517
Sfr303I CCGCGG 1 cut(s) 124
SgrAI CRCCGGYG 1 cut(s) 223
SgrBI CCGCGG 1 cut(s) 124
SinI GGWCC 1 cut(s) 483
Sse9I AATT 4 cut(s) 187, 560, 601, 620
StyD4I CCNGG 2 cut(s) 421, 590
TaiI ACGT 1 cut(s) 420
TaqI TCGA 2 cut(s) 504, 630
TaqII GACCGA 1 cut(s) 500
TasI AATT 4 cut(s) 187, 560, 601, 620
TauI GCSGC 3 cut(s) 114, 126, 411
TfiI GAWTC 1 cut(s) 237
TseFI GTSAC 2 cut(s) 413, 451
TseI GCWGC 2 cut(s) 289, 352
Tsp45I GTSAC 2 cut(s) 413, 451
TspDTI ATGAA 1 cut(s) 405
TspGWI ACGGA 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 483
XceI RCATGY 2 cut(s) 523, 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.