Rh2DG030300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003259
Physical Location & Seq
Forward (+)
1854470 .. 1857420
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG030300.1

Sequence Viewer

Length: 1134 bp
ATGTCTTGGCTCTTCACCTCTCTCCAACCCGACGCGGAAAACGACGCGTCGTTTCTCGGCCAAACCCTCACCCGCCAACTCCGCGGCGTCGCCACCTTCCTCGCTCCGCCGCCCTCCTCCTCCAAGTCCCTTCCCTCCTCCGATTCTCCACCGCCGTCGCCGTCTCCGTCGCCGGCGCTCTTGGGAGTCCGAAACGATCTCGCGGAATTGGGCGGGAGCTTCAAGACCGGCCTCTCGCTTCTCTCCGTCGCCGGAATCTCCAAATTGGCTTCGAACTTGCTCCCGGTTCGGAACGGAGCTCCGGAAGACGGCGGCGGCGGCGGTCCCGTCCCGGGGATTACCGATGAGATTCTCGGCTTCGTCGCTCAGATTTCGAAGCTTCCGGACTGTTGGACCGGCTTTCCTATACCACTGGACCACCATGATTTTAGCATGTCCAATGCTCAGAGAGAACATGCCTCCACTGTCGAACAATTGGTTCCGGGGTTTGCTGATCTGAGAGTCAGAGTTTGCAGTTCGATGAGTGAGGAGCAATTTTGGATGATCTATTTCTTGTTGCTGCTTCCAAGACTAAATGCAGATGATTTTGAGCTTCTGTCAACCCCCAAGATTGTTGAAGCAAGAAATGTACTTCTGCACAAGCTTCAAAGGAACACGCAGGTATCCACCTCTGAGGAGTCAACTCTTCATTCAACTGAAAAGGACACCCAGGTTGAGAAGACACAAGGGGGAGAATTCTCCTCTGCAGAAAAGAAAGATCTGACAGAGATTGTAAATACAGCTGAAAGGTTAAGAATCGACGATGAAGAGAGCACTGAGCAATGGTCCGAAGCAGCGAGCATTAGTTCTGGTACTTTTGTGGATGACCCAAAAAAACTTGAACATGAAGACGATATCTCATTCAGCGATTTAGAGGATGACGAGAATGACATTTCTACTAGATCGTCAGGCCTAAGGCCAGCAAAGGACGTTAGGGAATGTTCACCTAGTGGATCAAATCATTCAAATGACTGGGTTCGGCTGAATAGAAGTGCTGCGATTGATGGTGGCCATCAAAGGACTGGTACATCGAAAGAGAAAGATTCAGAAGGTGAGGAGTCGAATGACTGGCTTACTGTAGATAAGTTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

377

Amino Acids

41.07

Weight (kDa)

4.59

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 142 - 192 5.1e-07 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 649
AccII CGCG 4 cut(s) 35, 47, 84, 203
AccIII TCCGGA 2 cut(s) 301, 382
AclWI GGATC 1 cut(s) 1000
AcoI YGGCCR 2 cut(s) 58, 1048
AcsI RAATTY 1 cut(s) 734
AcyI GRCGYC 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 989
AfaI GTAC 3 cut(s) 630, 853, 1066
AfiI CCNNNNNNNGG 3 cut(s) 308, 332, 333
AflIII ACRYGT 1 cut(s) 45
AgsI TTSAA 6 cut(s) 223, 617, 647, 693, 881, 1005
AjnI CCWGG 1 cut(s) 708
AluBI AGCT 6 cut(s) 219, 299, 379, 592, 643, 782
AluI AGCT 6 cut(s) 219, 299, 379, 592, 643, 782
Alw21I GWGCWC 2 cut(s) 301, 815
Alw26I GTCTC 1 cut(s) 168
AlwI GGATC 1 cut(s) 1000
Ama87I CYCGRG 1 cut(s) 331
Aor13HI TCCGGA 2 cut(s) 301, 382
AoxI GGCC 5 cut(s) 58, 229, 949, 956, 1048
ApeKI GCWGC 3 cut(s) 559, 833, 1034
ApoI RAATTY 1 cut(s) 734
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 4 cut(s) 323, 393, 415, 825
AsuC2I CCSGG 4 cut(s) 284, 332, 333, 483
AsuHPI GGTGA 4 cut(s) 7, 61, 975, 1103
AsuII TTCGAA 2 cut(s) 272, 374
AvaI CYCGRG 1 cut(s) 331
AvaII GGWCC 4 cut(s) 323, 393, 415, 825
AxyI CCTNAGG 1 cut(s) 953
BaeI ACNNNNGTAYC 4 cut(s) 645, 678, 843, 876
BalI TGGCCA 1 cut(s) 1050
BanII GRGCYC 1 cut(s) 301
BbsI GAAGAC 3 cut(s) 312, 725, 894
Bbv12I GWGCWC 2 cut(s) 301, 815
BbvI GCAGC 3 cut(s) 546, 845, 1021
BccI CCATC 2 cut(s) 1037, 1059
BceAI ACGGC 3 cut(s) 139, 145, 325
BciT130I CCWGG 1 cut(s) 710
BciVI GTATCC 1 cut(s) 673
BcnI CCSGG 4 cut(s) 284, 332, 333, 483
BcoDI GTCTC 1 cut(s) 168
BfaI CTAG 2 cut(s) 939, 987
BfmI CTRYAG 2 cut(s) 744, 1116
BfoI RGCGCY 1 cut(s) 179
BfuAI ACCTGC 1 cut(s) 649
BfuI GTATCC 1 cut(s) 673
BglII AGATCT 1 cut(s) 757
BisI GCNGC 8 cut(s) 85, 110, 313, 316, 319, 560, 834, 1035
BlsI GCNGC 8 cut(s) 86, 111, 314, 317, 320, 561, 835, 1036
Bme1390I CCNGG 5 cut(s) 284, 332, 333, 483, 710
Bme18I GGWCC 4 cut(s) 323, 393, 415, 825
BmeT110I CYCGRG 1 cut(s) 331
BmgT120I GGNCC 4 cut(s) 323, 393, 415, 825
BmiI GGNNCC 2 cut(s) 325, 480
BmrFI CCNGG 5 cut(s) 284, 332, 333, 483, 710
BmrI ACTGGG 1 cut(s) 1021
BmuI ACTGGG 1 cut(s) 1021
BpiI GAAGAC 3 cut(s) 312, 725, 894
Bpu14I TTCGAA 2 cut(s) 272, 374
BpuMI CCSGG 4 cut(s) 284, 332, 333, 483
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 5 cut(s) 82, 331, 332, 482, 708
BsaWI WCCGGW 2 cut(s) 301, 382
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 332, 333
Bse118I RCCGGY 3 cut(s) 172, 227, 395
Bse1I ACTGG 4 cut(s) 417, 1016, 1066, 1112
Bse21I CCTNAGG 1 cut(s) 953
Bse3DI GCAATG 1 cut(s) 827
BseAI TCCGGA 2 cut(s) 301, 382
BseBI CCWGG 1 cut(s) 710
BseDI CCNNGG 5 cut(s) 82, 331, 332, 482, 708
BseGI GGATG 3 cut(s) 546, 868, 922
BseLI CCNNNNNNNGG 3 cut(s) 308, 332, 333
BseMI GCAATG 1 cut(s) 827
BseMII CTCAG 5 cut(s) 380, 458, 488, 663, 807
BseNI ACTGG 4 cut(s) 417, 1016, 1066, 1112
BseRI GAGGAG 7 cut(s) 106, 109, 127, 542, 689, 730, 1109
BseXI GCAGC 3 cut(s) 546, 845, 1021
BsgI GTGCAG 1 cut(s) 620
Bsh1236I CGCG 4 cut(s) 35, 47, 84, 203
BshFI GGCC 5 cut(s) 60, 231, 951, 958, 1050
BsiHKAI GWGCWC 2 cut(s) 301, 815
BsiHKCI CYCGRG 1 cut(s) 331
BsiSI CCGG 9 cut(s) 173, 228, 252, 284, 302, 332, 383, 396, 482
BslFI GGGAC 3 cut(s) 112, 309, 314
BslI CCNNNNNNNGG 3 cut(s) 308, 332, 333
BsmAI GTCTC 1 cut(s) 168
BsmBI CGTCTC 1 cut(s) 168
BsmFI GGGAC 3 cut(s) 112, 309, 314
BsnI GGCC 5 cut(s) 60, 231, 951, 958, 1050
BsoBI CYCGRG 1 cut(s) 331
Bsp119I TTCGAA 2 cut(s) 272, 374
Bsp1286I GDGCHC 2 cut(s) 301, 815
Bsp13I TCCGGA 2 cut(s) 301, 382
Bsp143I GATC 6 cut(s) 196, 493, 543, 757, 941, 992
BspANI GGCC 5 cut(s) 60, 231, 951, 958, 1050
BspCNI CTCAG 5 cut(s) 379, 457, 489, 664, 808
BspEI TCCGGA 2 cut(s) 301, 382
BspFNI CGCG 4 cut(s) 35, 47, 84, 203
BspLI GGNNCC 2 cut(s) 325, 480
BspMAI CTGCAG 1 cut(s) 748
BspMI ACCTGC 1 cut(s) 649
BspPI GGATC 1 cut(s) 1000
BspQI GCTCTTC 1 cut(s) 17
BspT104I TTCGAA 2 cut(s) 272, 374
BsrDI GCAATG 1 cut(s) 827
BsrFI RCCGGY 3 cut(s) 172, 227, 395
BsrI ACTGG 4 cut(s) 417, 1016, 1066, 1112
BssAI RCCGGY 3 cut(s) 172, 227, 395
BssECI CCNNGG 5 cut(s) 82, 331, 332, 482, 708
BssMI GATC 6 cut(s) 196, 493, 543, 757, 941, 992
BssNI GRCGYC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 3 cut(s) 389, 466, 1117
Bst6I CTCTTC 3 cut(s) 17, 690, 801
BstACI GRCGYC 1 cut(s) 87
BstBI TTCGAA 2 cut(s) 272, 374
BstC8I GCNNGC 3 cut(s) 174, 838, 960
BstDEI CTNAG 6 cut(s) 366, 444, 497, 672, 816, 953
BstDSI CCRYGG 1 cut(s) 82
BstF5I GGATG 3 cut(s) 546, 868, 922
BstFNI CGCG 4 cut(s) 35, 47, 84, 203
BstH2I RGCGCY 1 cut(s) 179
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 6 cut(s) 199, 496, 546, 760, 944, 995
BstMAI GTCTC 1 cut(s) 168
BstMBI GATC 6 cut(s) 196, 493, 543, 757, 941, 992
BstMWI GCNNNNNNNGC 2 cut(s) 81, 318
BstNI CCWGG 1 cut(s) 710
BstNSI RCATGY 2 cut(s) 436, 458
BstSCI CCNGG 5 cut(s) 282, 330, 331, 481, 708
BstSFI CTRYAG 2 cut(s) 744, 1116
BstUI CGCG 4 cut(s) 35, 47, 84, 203
BstV1I GCAGC 3 cut(s) 546, 845, 1021
BstV2I GAAGAC 3 cut(s) 312, 725, 894
BstX2I RGATCY 1 cut(s) 757
BstYI RGATCY 1 cut(s) 757
Bsu36I CCTNAGG 1 cut(s) 953
BsuI GTATCC 1 cut(s) 673
BsuRI GGCC 5 cut(s) 60, 231, 951, 958, 1050
BtgI CCRYGG 1 cut(s) 82
BtsCI GGATG 3 cut(s) 546, 868, 922
BtsIMutI CAGTG 3 cut(s) 410, 462, 813
BveI ACCTGC 1 cut(s) 649
Cac8I GCNNGC 3 cut(s) 174, 838, 960
CfoI GCGC 1 cut(s) 178
Cfr10I RCCGGY 3 cut(s) 172, 227, 395
Cfr13I GGNCC 4 cut(s) 323, 393, 415, 825
Cfr42I CCGCGG 1 cut(s) 85
Cfr9I CCCGGG 1 cut(s) 331
CseI GACGC 4 cut(s) 36, 41, 53, 76
Csp6I GTAC 3 cut(s) 629, 852, 1065
CviAII CATG 4 cut(s) 422, 433, 455, 884
CviQI GTAC 3 cut(s) 629, 852, 1065
DdeI CTNAG 6 cut(s) 366, 444, 497, 672, 816, 953
DpnI GATC 6 cut(s) 198, 495, 545, 759, 943, 994
DpnII GATC 6 cut(s) 196, 493, 543, 757, 941, 992
DraIII CACNNNGTG 1 cut(s) 989
EaeI YGGCCR 2 cut(s) 58, 1048
Eam1104I CTCTTC 3 cut(s) 17, 690, 801
EarI CTCTTC 3 cut(s) 17, 690, 801
EciI GGCGGA 1 cut(s) 96
Ecl136II GAGCTC 1 cut(s) 299
Eco147I AGGCCT 1 cut(s) 951
Eco24I GRGCYC 1 cut(s) 301
Eco32I GATATC 1 cut(s) 895
Eco47I GGWCC 4 cut(s) 323, 393, 415, 825
Eco53kI GAGCTC 1 cut(s) 299
Eco81I CCTNAGG 1 cut(s) 953
Eco88I CYCGRG 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 299
EcoRI GAATTC 1 cut(s) 734
EcoRII CCWGG 1 cut(s) 708
EcoRV GATATC 1 cut(s) 895
EcoT38I GRGCYC 1 cut(s) 301
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 4 cut(s) 425, 436, 458, 887
FaiI YATR 5 cut(s) 407, 423, 434, 456, 885
FaqI GGGAC 3 cut(s) 112, 309, 314
FatI CATG 4 cut(s) 421, 432, 454, 883
FauI CCCGC 2 cut(s) 80, 206
Fnu4HI GCNGC 8 cut(s) 85, 110, 313, 316, 319, 560, 834, 1035
FokI GGATG 3 cut(s) 553, 875, 929
FriOI GRGCYC 1 cut(s) 301
Fsp4HI GCNGC 8 cut(s) 85, 110, 313, 316, 319, 560, 834, 1035
FspBI CTAG 2 cut(s) 939, 987
GlaI GCGC 1 cut(s) 177
GluI GCNGC 8 cut(s) 85, 110, 313, 316, 319, 560, 834, 1035
HaeII RGCGCY 1 cut(s) 179
HaeIII GGCC 5 cut(s) 60, 231, 951, 958, 1050
HapII CCGG 9 cut(s) 173, 228, 252, 284, 302, 332, 383, 396, 482
HgaI GACGC 4 cut(s) 36, 41, 53, 76
HhaI GCGC 1 cut(s) 178
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 4 cut(s) 425, 436, 458, 887
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 2 cut(s) 600, 681
HindII GTYRAC 2 cut(s) 600, 681
HindIII AAGCTT 2 cut(s) 377, 641
HinfI GANTC 9 cut(s) 143, 186, 255, 349, 501, 677, 795, 1082, 1097
HpaII CCGG 9 cut(s) 173, 228, 252, 284, 302, 332, 383, 396, 482
HphI GGTGA 4 cut(s) 7, 61, 975, 1103
Hpy166II GTNNAC 3 cut(s) 600, 681, 983
Hpy188III TCNNGA 3 cut(s) 223, 302, 383
Hpy8I GTNNAC 3 cut(s) 600, 681, 983
Hpy99I CGWCG 9 cut(s) 35, 47, 52, 92, 160, 172, 251, 365, 803
HpyAV CCTTC 3 cut(s) 106, 140, 1082
HpyCH4III ACNGT 3 cut(s) 389, 466, 1117
HpyCH4IV ACGT 1 cut(s) 969
HpyCH4V TGCA 4 cut(s) 513, 578, 637, 746
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 318
HpyF3I CTNAG 6 cut(s) 366, 444, 497, 672, 816, 953
HpySE526I ACGT 1 cut(s) 969
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 4 cut(s) 425, 436, 458, 887
HspAI GCGC 1 cut(s) 176
Kpn2I TCCGGA 2 cut(s) 301, 382
KroI GCCGGC 1 cut(s) 172
KroNI GCCGGC 1 cut(s) 174
KspI CCGCGG 1 cut(s) 85
Kzo9I GATC 6 cut(s) 196, 493, 543, 757, 941, 992
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 6 cut(s) 109, 216, 285, 296, 304, 529
Lsp1109I GCAGC 3 cut(s) 546, 845, 1021
MaeI CTAG 2 cut(s) 939, 987
MaeII ACGT 1 cut(s) 969
MalI GATC 6 cut(s) 198, 495, 545, 759, 943, 994
MboI GATC 6 cut(s) 196, 493, 543, 757, 941, 992
MboII GAAGA 6 cut(s) 4, 317, 677, 730, 818, 899
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 1 cut(s) 757
MhlI GDGCHC 2 cut(s) 301, 815
MlsI TGGCCA 1 cut(s) 1050
MluCI AATT 5 cut(s) 206, 263, 473, 533, 734
MluI ACGCGT 1 cut(s) 45
MluNI TGGCCA 1 cut(s) 1050
MlyI GAGTC 4 cut(s) 195, 510, 686, 1106
MmeI TCCRAC 2 cut(s) 49, 371
Mox20I TGGCCA 1 cut(s) 1050
MreI CGCCGGCG 1 cut(s) 172
MroI TCCGGA 2 cut(s) 301, 382
MroNI GCCGGC 1 cut(s) 172
MscI TGGCCA 1 cut(s) 1050
MseI TTAA 1 cut(s) 791
MslI CAYNNNNRTG 1 cut(s) 1005
Msp20I TGGCCA 1 cut(s) 1050
MspA1I CMGCKG 2 cut(s) 84, 782
MspI CCGG 9 cut(s) 173, 228, 252, 284, 302, 332, 383, 396, 482
MspR9I CCNGG 5 cut(s) 284, 332, 333, 483, 710
MunI CAATTG 1 cut(s) 473
MvaI CCWGG 1 cut(s) 710
MvnI CGCG 4 cut(s) 35, 47, 84, 203
MwoI GCNNNNNNNGC 2 cut(s) 81, 318
NaeI GCCGGC 1 cut(s) 174
NciI CCSGG 4 cut(s) 284, 332, 333, 483
NdeII GATC 6 cut(s) 196, 493, 543, 757, 941, 992
NgoMIV GCCGGC 1 cut(s) 172
NlaIII CATG 4 cut(s) 425, 436, 458, 887
NlaIV GGNNCC 2 cut(s) 325, 480
NmeAIII GCCGAG 2 cut(s) 36, 333
NspI RCATGY 2 cut(s) 436, 458
NspV TTCGAA 2 cut(s) 272, 374
PceI AGGCCT 1 cut(s) 951
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 164
PdiI GCCGGC 1 cut(s) 174
PfeI GAWTC 5 cut(s) 143, 255, 349, 795, 1082
PkrI GCNGC 8 cut(s) 86, 111, 314, 317, 320, 561, 835, 1036
PleI GAGTC 4 cut(s) 194, 509, 685, 1105
PpsI GAGTC 4 cut(s) 194, 509, 685, 1105
Psp124BI GAGCTC 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 708
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 2 cut(s) 325, 480
PspPI GGNCC 4 cut(s) 323, 393, 415, 825
PstI CTGCAG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 757
PvuII CAGCTG 1 cut(s) 782
RsaI GTAC 3 cut(s) 630, 853, 1066
RsaNI GTAC 3 cut(s) 629, 852, 1065
RseI CAYNNNNRTG 1 cut(s) 1005
SacI GAGCTC 1 cut(s) 301
SacII CCGCGG 1 cut(s) 85
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 791
SatI GCNGC 8 cut(s) 85, 110, 313, 316, 319, 560, 834, 1035
Sau3AI GATC 6 cut(s) 196, 493, 543, 757, 941, 992
Sau96I GGNCC 4 cut(s) 323, 393, 415, 825
SchI GAGTC 4 cut(s) 195, 510, 686, 1106
ScrFI CCNGG 5 cut(s) 284, 332, 333, 483, 710
SduI GDGCHC 2 cut(s) 301, 815
SfcI CTRYAG 2 cut(s) 744, 1116
Sfr303I CCGCGG 1 cut(s) 85
SfuI TTCGAA 2 cut(s) 272, 374
SgrAI CRCCGGYG 1 cut(s) 172
SgrBI CCGCGG 1 cut(s) 85
SinI GGWCC 4 cut(s) 323, 393, 415, 825
SmaI CCCGGG 1 cut(s) 333
SmiMI CAYNNNNRTG 1 cut(s) 1005
Sse9I AATT 5 cut(s) 206, 263, 473, 533, 734
SseBI AGGCCT 1 cut(s) 951
SspMI CTAG 2 cut(s) 939, 987
SstI GAGCTC 1 cut(s) 301
StuI AGGCCT 1 cut(s) 951
StyD4I CCNGG 5 cut(s) 282, 330, 331, 481, 708
TaaI ACNGT 3 cut(s) 389, 466, 1117
TaiI ACGT 1 cut(s) 972
TaqI TCGA 7 cut(s) 272, 374, 468, 518, 798, 1070, 1100
TasI AATT 5 cut(s) 206, 263, 473, 533, 734
TatI WGTACW 1 cut(s) 628
TauI GCSGC 5 cut(s) 87, 112, 315, 318, 321
TfiI GAWTC 5 cut(s) 143, 255, 349, 795, 1082
Tru1I TTAA 1 cut(s) 791
Tru9I TTAA 1 cut(s) 791
TscAI CASTG 3 cut(s) 417, 469, 820
TseI GCWGC 3 cut(s) 559, 833, 1034
TspDTI ATGAA 3 cut(s) 677, 819, 900
TspGWI ACGGA 3 cut(s) 156, 235, 309
TspMI CCCGGG 1 cut(s) 331
TspRI CASTG 3 cut(s) 417, 469, 820
VpaK11BI GGWCC 4 cut(s) 323, 393, 415, 825
XapI RAATTY 1 cut(s) 734
XceI RCATGY 2 cut(s) 436, 458
XcmI CCANNNNNNNNNTGG 1 cut(s) 1058
XmaI CCCGGG 1 cut(s) 331
XspI CTAG 2 cut(s) 939, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.