MD02G1023000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003259
Physical Location & Seq
Forward (+)
1605870 .. 1608150
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1023000.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGTCTTGGCTCTTCAGCTCTCCCCGCCAACCAGAAGACGACGACGACGACCAGCGTGATGTAGAACCCAAGCAACCCCAAAACGACCTGTCGTTTCTCAGCCAAACCCTCGGCCGCCAACTCCGCGGCGTCGCCACCTTCCTCGCTCCACCTCCATCACCGCCATCCAACGACGCCCTTCCCTCGTCCAATTCCCAGCCCCAGTCGCAATCGCAATCTCAATCGCCGGCGCTTTTGGGAATCCGGAATGATCTGGTGGAGATCGGCGGGAGCTTCAAAACCGGCCTCTCGCTGCTCTCTACTAACAGCAACAAAGCCGTCACTGAGATCTCCAAGTTCGCTTCGGGCTTGTTGCAGCTTCAAAACGAAGCTTCAGAAGAAGGTGGAGCTGATGATGACGGCGACGATGGCGGCGGTCACGTGCCGGGGATCACGGACGAGGTTCTCGGTTTCGTCACCGATATTTCGGTGATGCCGGACTGCTGGACCGACTTTCCAATGACGCTCGATCATGATTTCAGCATGTCGGATGCTCAGAGAGAGCATGTGTCCGCTGTTTTACAATTGGTTCCGGGATTTGCAGATCTAAGAGCCCGGCTTTGCAATTCCATGAGTGAGGAGCAATTTTGGATGATCTATTTCTTGTTGTTGCTCCCGAGACTAAACGAACATGATTTTGAGCTTCTAGCAACGCCCAAGATTGTTGAAGCAAGAGACGTACTTCTGCACAAACTGCATAACAAGAGGAACACACGGGAGGATGCCCCAGAGAAGTCAATTCTCGTTTCACCTAAAAATCACATCCAGGATGGGAAGACACAAGGGGAGGGAAGCTCATCTCAAGAAAAGGAAGCCTCGACTGAAATACTTAACACGACAGAAAGGTTAAAGACCGATGACGAAGAGGGCACTGAGCAGTGGTCTGAAGGTGCAAGTATTAGCTCTGGCACTTTTGTGGATGGCCAAAGAAAACACGAACCTGAAGACGATATCTCATTCAGCGATTTAGAGGACGAAGAGAATGACGTTTCTAGTAGACAATCAGGACTAAGGCAAGGACCGTATGCCAGGGCATGTTCACCCAATGGATCAAATGATTGGGTTCAGCTGAATCGAAGCTCGGAAAACGAGGGTGGCCGGCAAAAGGCTGGTCGGTCAAAGGGGAAAGATTCAGAAGGTGAGGATTCAAGTGACTGGCTTGCGGTAGATGAATATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

407

Amino Acids

44.66

Weight (kDa)

4.41

Isoelectric Point (pI)

69.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 170 - 224 8.5e-08 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1036
AccII CGCG 1 cut(s) 126
AccIII TCCGGA 1 cut(s) 243
AclWI GGATC 2 cut(s) 437, 1096
AcoI YGGCCR 3 cut(s) 112, 961, 1135
AcuI CTGAAG 3 cut(s) 357, 945, 1002
AcvI CACGTG 1 cut(s) 421
AcyI GRCGYC 2 cut(s) 129, 174
AfaI GTAC 1 cut(s) 720
AfiI CCNNNNNNNGG 1 cut(s) 1144
AgsI TTSAA 4 cut(s) 277, 362, 707, 1188
AjnI CCWGG 2 cut(s) 804, 1067
AleI CACNNNNGTG 1 cut(s) 953
Alw26I GTCTC 2 cut(s) 652, 708
AlwI GGATC 2 cut(s) 437, 1096
Ama87I CYCGRG 1 cut(s) 655
Aor13HI TCCGGA 1 cut(s) 243
AoxI GGCC 4 cut(s) 112, 283, 961, 1135
ApeKI GCWGC 2 cut(s) 292, 355
ArsI GACNNNNNNTTYG 2 cut(s) 1135, 1167
AspLEI GCGC 1 cut(s) 232
AspS9I GGNCC 2 cut(s) 486, 1058
AsuC2I CCSGG 3 cut(s) 426, 573, 595
AsuHPI GGTGA 6 cut(s) 150, 448, 481, 780, 1071, 1190
AvaI CYCGRG 1 cut(s) 655
AvaII GGWCC 2 cut(s) 486, 1058
BaeGI GKGCMC 1 cut(s) 911
BalI TGGCCA 1 cut(s) 963
BanII GRGCYC 1 cut(s) 595
BbrPI CACGTG 1 cut(s) 421
BbsI GAAGAC 3 cut(s) 42, 821, 990
BbvI GCAGC 2 cut(s) 279, 367
BccI CCATC 5 cut(s) 163, 172, 401, 803, 953
BceAI ACGGC 2 cut(s) 302, 415
BciT130I CCWGG 2 cut(s) 806, 1069
BcnI CCSGG 3 cut(s) 426, 573, 595
BcoDI GTCTC 2 cut(s) 652, 708
BfaI CTAG 2 cut(s) 686, 1032
BfoI RGCGCY 1 cut(s) 233
BglII AGATCT 2 cut(s) 327, 583
BisI GCNGC 5 cut(s) 115, 127, 293, 356, 412
BlsI GCNGC 5 cut(s) 116, 128, 294, 357, 413
Bme1390I CCNGG 5 cut(s) 426, 573, 595, 806, 1069
Bme18I GGWCC 2 cut(s) 486, 1058
BmeT110I CYCGRG 1 cut(s) 655
BmgT120I GGNCC 2 cut(s) 486, 1058
BmiI GGNNCC 1 cut(s) 570
BmrFI CCNGG 5 cut(s) 426, 573, 595, 806, 1069
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 3 cut(s) 462, 520, 751
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 3 cut(s) 42, 821, 990
BplI GAGNNNNNCTC 2 cut(s) 818, 850
BpuEI CTTGAG 1 cut(s) 825
BpuMI CCSGG 3 cut(s) 426, 573, 595
BsaAI YACGTR 1 cut(s) 421
BsaHI GRCGYC 2 cut(s) 129, 174
BsaJI CCNNGG 4 cut(s) 109, 124, 425, 1068
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 1144
Bse118I RCCGGY 3 cut(s) 226, 281, 1137
Bse1I ACTGG 2 cut(s) 202, 1199
BseAI TCCGGA 1 cut(s) 243
BseBI CCWGG 2 cut(s) 806, 1069
BseDI CCNNGG 4 cut(s) 109, 124, 425, 1068
BseGI GGATG 7 cut(s) 164, 535, 636, 766, 801, 814, 964
BseLI CCNNNNNNNGG 1 cut(s) 1144
BseMII CTCAG 4 cut(s) 112, 315, 548, 903
BseNI ACTGG 2 cut(s) 202, 1199
BseRI GAGGAG 1 cut(s) 632
BseSI GKGCMC 1 cut(s) 911
BseX3I CGGCCG 1 cut(s) 112
BseXI GCAGC 2 cut(s) 279, 367
BseYI CCCAGC 1 cut(s) 195
BsgI GTGCAG 1 cut(s) 710
Bsh1236I CGCG 1 cut(s) 126
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 4 cut(s) 114, 285, 963, 1137
BsiEI CGRYCG 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 655
BsiSI CCGG 8 cut(s) 227, 244, 282, 425, 476, 572, 595, 1138
BslI CCNNNNNNNGG 1 cut(s) 1144
BsmAI GTCTC 2 cut(s) 652, 708
BsmBI CGTCTC 1 cut(s) 708
BsnI GGCC 4 cut(s) 114, 285, 963, 1137
BsoBI CYCGRG 1 cut(s) 655
Bsp1286I GDGCHC 2 cut(s) 595, 911
Bsp13I TCCGGA 1 cut(s) 243
Bsp143I GATC 8 cut(s) 250, 261, 327, 429, 508, 583, 633, 1088
BspANI GGCC 4 cut(s) 114, 285, 963, 1137
BspCNI CTCAG 4 cut(s) 111, 316, 547, 904
BspEI TCCGGA 1 cut(s) 243
BspFNI CGCG 1 cut(s) 126
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 1 cut(s) 570
BspPI GGATC 2 cut(s) 437, 1096
BspQI GCTCTTC 1 cut(s) 17
BsrFI RCCGGY 3 cut(s) 226, 281, 1137
BsrI ACTGG 2 cut(s) 202, 1199
BssAI RCCGGY 3 cut(s) 226, 281, 1137
BssECI CCNNGG 4 cut(s) 109, 124, 425, 1068
BssMI GATC 8 cut(s) 250, 261, 327, 429, 508, 583, 633, 1088
BssNI GRCGYC 2 cut(s) 129, 174
Bst2UI CCWGG 2 cut(s) 806, 1069
Bst4CI ACNGT 1 cut(s) 1062
Bst6I CTCTTC 3 cut(s) 17, 897, 1011
BstACI GRCGYC 2 cut(s) 129, 174
BstAPI GCANNNNNTGC 1 cut(s) 733
BstBAI YACGTR 1 cut(s) 421
BstC8I GCNNGC 3 cut(s) 228, 1139, 1200
BstDEI CTNAG 6 cut(s) 98, 324, 534, 587, 912, 1049
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 7 cut(s) 164, 535, 636, 766, 801, 814, 964
BstFNI CGCG 1 cut(s) 126
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstKTI GATC 8 cut(s) 253, 264, 330, 432, 511, 586, 636, 1091
BstMAI GTCTC 2 cut(s) 652, 708
BstMBI GATC 8 cut(s) 250, 261, 327, 429, 508, 583, 633, 1088
BstMCI CGRYCG 1 cut(s) 115
BstMWI GCNNNNNNNGC 5 cut(s) 24, 123, 205, 408, 733
BstNI CCWGG 2 cut(s) 806, 1069
BstNSI RCATGY 3 cut(s) 526, 548, 1077
BstSCI CCNGG 5 cut(s) 424, 571, 593, 804, 1067
BstSLI GKGCMC 1 cut(s) 911
BstUI CGCG 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 279, 367
BstV2I GAAGAC 3 cut(s) 42, 821, 990
BstX2I RGATCY 2 cut(s) 327, 583
BstYI RGATCY 2 cut(s) 327, 583
BstZI CGGCCG 1 cut(s) 112
BsuRI GGCC 4 cut(s) 114, 285, 963, 1137
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 7 cut(s) 164, 535, 636, 766, 801, 814, 964
BtsI GCAGTG 1 cut(s) 923
BtsIMutI CAGTG 3 cut(s) 321, 909, 923
Cac8I GCNNGC 3 cut(s) 228, 1139, 1200
CciI TCATGA 1 cut(s) 511
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 3 cut(s) 226, 281, 1137
Cfr13I GGNCC 2 cut(s) 486, 1058
Cfr42I CCGCGG 1 cut(s) 127
CseI GACGC 3 cut(s) 118, 182, 511
Csp6I GTAC 1 cut(s) 719
CviAII CATG 6 cut(s) 512, 523, 545, 610, 671, 1074
CviQI GTAC 1 cut(s) 719
DdeI CTNAG 6 cut(s) 98, 324, 534, 587, 912, 1049
DpnI GATC 8 cut(s) 252, 263, 329, 431, 510, 585, 635, 1090
DpnII GATC 8 cut(s) 250, 261, 327, 429, 508, 583, 633, 1088
EaeI YGGCCR 3 cut(s) 112, 961, 1135
EagI CGGCCG 1 cut(s) 112
Eam1104I CTCTTC 3 cut(s) 17, 897, 1011
EarI CTCTTC 3 cut(s) 17, 897, 1011
EclXI CGGCCG 1 cut(s) 112
Eco24I GRGCYC 1 cut(s) 595
Eco32I GATATC 1 cut(s) 991
Eco47I GGWCC 2 cut(s) 486, 1058
Eco52I CGGCCG 1 cut(s) 112
Eco57I CTGAAG 3 cut(s) 357, 945, 1002
Eco72I CACGTG 1 cut(s) 421
Eco88I CYCGRG 1 cut(s) 655
EcoRII CCWGG 2 cut(s) 804, 1067
EcoRV GATATC 1 cut(s) 991
EcoT38I GRGCYC 1 cut(s) 595
Esp3I CGTCTC 1 cut(s) 708
FaeI CATG 6 cut(s) 515, 526, 548, 613, 674, 1077
FaiI YATR 9 cut(s) 513, 524, 546, 611, 672, 738, 1065, 1075, 1215
FatI CATG 6 cut(s) 511, 522, 544, 609, 670, 1073
FauI CCCGC 2 cut(s) 32, 260
FblI GTMKAC 1 cut(s) 1036
Fnu4HI GCNGC 5 cut(s) 115, 127, 293, 356, 412
FokI GGATG 7 cut(s) 151, 542, 643, 773, 788, 821, 971
FriOI GRGCYC 1 cut(s) 595
Fsp4HI GCNGC 5 cut(s) 115, 127, 293, 356, 412
FspBI CTAG 2 cut(s) 686, 1032
GlaI GCGC 1 cut(s) 231
GluI GCNGC 5 cut(s) 115, 127, 293, 356, 412
GsaI CCCAGC 1 cut(s) 199
HaeII RGCGCY 1 cut(s) 233
HaeIII GGCC 4 cut(s) 114, 285, 963, 1137
HapII CCGG 8 cut(s) 227, 244, 282, 425, 476, 572, 595, 1138
HgaI GACGC 3 cut(s) 118, 182, 511
HhaI GCGC 1 cut(s) 232
Hin1I GRCGYC 2 cut(s) 129, 174
Hin1II CATG 6 cut(s) 515, 526, 548, 613, 674, 1077
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HindIII AAGCTT 1 cut(s) 369
HinfI GANTC 4 cut(s) 240, 1111, 1169, 1184
HpaII CCGG 8 cut(s) 227, 244, 282, 425, 476, 572, 595, 1138
HphI GGTGA 6 cut(s) 150, 448, 481, 780, 1071, 1190
Hpy166II GTNNAC 2 cut(s) 1037, 1079
Hpy188I TCNGA 6 cut(s) 376, 529, 537, 925, 1123, 1174
Hpy188III TCNNGA 5 cut(s) 244, 512, 655, 842, 1044
Hpy8I GTNNAC 2 cut(s) 1037, 1079
Hpy99I CGWCG 6 cut(s) 44, 47, 50, 134, 176, 407
HpyAV CCTTC 5 cut(s) 148, 188, 374, 920, 1169
HpyCH4III ACNGT 1 cut(s) 1062
HpyCH4IV ACGT 3 cut(s) 420, 717, 1026
HpyCH4V TGCA 6 cut(s) 355, 581, 603, 727, 736, 932
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 123, 205, 408, 733
HpyF3I CTNAG 6 cut(s) 98, 324, 534, 587, 912, 1049
HpySE526I ACGT 3 cut(s) 420, 717, 1026
Hsp92I GRCGYC 2 cut(s) 129, 174
Hsp92II CATG 6 cut(s) 515, 526, 548, 613, 674, 1077
HspAI GCGC 1 cut(s) 230
Kpn2I TCCGGA 1 cut(s) 243
KroI GCCGGC 2 cut(s) 226, 1137
KroNI GCCGGC 2 cut(s) 228, 1139
KspI CCGCGG 1 cut(s) 127
Kzo9I GATC 8 cut(s) 250, 261, 327, 429, 508, 583, 633, 1088
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 5 cut(s) 151, 270, 386, 619, 657
Lsp1109I GCAGC 2 cut(s) 279, 367
LweI GCATC 3 cut(s) 462, 520, 751
MaeI CTAG 2 cut(s) 686, 1032
MaeII ACGT 3 cut(s) 420, 717, 1026
MaeIII GTNAC 4 cut(s) 319, 416, 454, 1190
MalI GATC 8 cut(s) 252, 263, 329, 431, 510, 585, 635, 1090
MboI GATC 8 cut(s) 250, 261, 327, 429, 508, 583, 633, 1088
MboII GAAGA 7 cut(s) 4, 47, 389, 826, 914, 995, 1028
MfeI CAATTG 1 cut(s) 563
MflI RGATCY 2 cut(s) 327, 583
MhlI GDGCHC 2 cut(s) 595, 911
MlsI TGGCCA 1 cut(s) 963
MluCI AATT 5 cut(s) 190, 563, 604, 623, 777
MluNI TGGCCA 1 cut(s) 963
MmeI TCCRAC 2 cut(s) 192, 507
Mox20I TGGCCA 1 cut(s) 963
MreI CGCCGGCG 1 cut(s) 226
MroI TCCGGA 1 cut(s) 243
MroNI GCCGGC 2 cut(s) 226, 1137
MscI TGGCCA 1 cut(s) 963
MseI TTAA 2 cut(s) 870, 887
MslI CAYNNNNRTG 1 cut(s) 953
Msp20I TGGCCA 1 cut(s) 963
MspA1I CMGCKG 3 cut(s) 126, 554, 1108
MspI CCGG 8 cut(s) 227, 244, 282, 425, 476, 572, 595, 1138
MspR9I CCNGG 5 cut(s) 426, 573, 595, 806, 1069
MunI CAATTG 1 cut(s) 563
MvaI CCWGG 2 cut(s) 806, 1069
MvnI CGCG 1 cut(s) 126
MwoI GCNNNNNNNGC 5 cut(s) 24, 123, 205, 408, 733
NaeI GCCGGC 2 cut(s) 228, 1139
NciI CCSGG 3 cut(s) 426, 573, 595
NdeII GATC 8 cut(s) 250, 261, 327, 429, 508, 583, 633, 1088
NgoMIV GCCGGC 2 cut(s) 226, 1137
NlaIII CATG 6 cut(s) 515, 526, 548, 613, 674, 1077
NlaIV GGNNCC 1 cut(s) 570
NmeAIII GCCGAG 1 cut(s) 90
NmuCI GTSAC 4 cut(s) 319, 416, 454, 1190
NspI RCATGY 3 cut(s) 526, 548, 1077
OliI CACNNNNGTG 1 cut(s) 953
PagI TCATGA 1 cut(s) 511
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PdiI GCCGGC 2 cut(s) 228, 1139
PfeI GAWTC 4 cut(s) 240, 1111, 1169, 1184
PfoI TCCNGGA 2 cut(s) 571, 804
PkrI GCNGC 5 cut(s) 116, 128, 294, 357, 413
PmaCI CACGTG 1 cut(s) 421
PmlI CACGTG 1 cut(s) 421
Ppu21I YACGTR 1 cut(s) 421
Psp6I CCWGG 2 cut(s) 804, 1067
PspCI CACGTG 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 195
PspGI CCWGG 2 cut(s) 804, 1067
PspN4I GGNNCC 1 cut(s) 570
PspPI GGNCC 2 cut(s) 486, 1058
PsuI RGATCY 2 cut(s) 327, 583
PvuII CAGCTG 1 cut(s) 1108
RsaI GTAC 1 cut(s) 720
RsaNI GTAC 1 cut(s) 719
RseI CAYNNNNRTG 1 cut(s) 953
SacII CCGCGG 1 cut(s) 127
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 2 cut(s) 870, 887
SatI GCNGC 5 cut(s) 115, 127, 293, 356, 412
Sau3AI GATC 8 cut(s) 250, 261, 327, 429, 508, 583, 633, 1088
Sau96I GGNCC 2 cut(s) 486, 1058
ScrFI CCNGG 5 cut(s) 426, 573, 595, 806, 1069
SduI GDGCHC 2 cut(s) 595, 911
SfaNI GCATC 3 cut(s) 462, 520, 751
Sfr303I CCGCGG 1 cut(s) 127
SgrAI CRCCGGYG 1 cut(s) 226
SgrBI CCGCGG 1 cut(s) 127
SinI GGWCC 2 cut(s) 486, 1058
SmiMI CAYNNNNRTG 1 cut(s) 953
SmlI CTYRAG 1 cut(s) 840
SmoI CTYRAG 1 cut(s) 840
Sse9I AATT 5 cut(s) 190, 563, 604, 623, 777
SspMI CTAG 2 cut(s) 686, 1032
StyD4I CCNGG 5 cut(s) 424, 571, 593, 804, 1067
TaaI ACNGT 1 cut(s) 1062
TaiI ACGT 3 cut(s) 423, 720, 1029
TaqI TCGA 3 cut(s) 507, 857, 1114
TaqII GACCGA 3 cut(s) 503, 908, 1143
TasI AATT 5 cut(s) 190, 563, 604, 623, 777
TauI GCSGC 3 cut(s) 117, 129, 414
TfiI GAWTC 4 cut(s) 240, 1111, 1169, 1184
Tru1I TTAA 2 cut(s) 870, 887
Tru9I TTAA 2 cut(s) 870, 887
TscAI CASTG 3 cut(s) 328, 916, 923
TseFI GTSAC 4 cut(s) 319, 416, 454, 1190
TseI GCWGC 2 cut(s) 292, 355
Tsp45I GTSAC 4 cut(s) 319, 416, 454, 1190
TspGWI ACGGA 1 cut(s) 449
TspRI CASTG 3 cut(s) 328, 916, 923
VpaK11BI GGWCC 2 cut(s) 486, 1058
XceI RCATGY 3 cut(s) 526, 548, 1077
XmiI GTMKAC 1 cut(s) 1036
XspI CTAG 2 cut(s) 686, 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.