MD15G1165700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003259
Physical Location & Seq
Forward (+)
12470312 .. 12472755
2444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1165700.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGTCTTGGCTCTTCAGCTCTCCCCGCCAACCCGACGACGACGACCCGCGTGATGTAGAACAACCCAACCAACCGCAAAACGACCTGTCGTTTCTCCGCCAAACCCTCGGCCGCCAAATCCGCGGCGTCGCCACCTTCCTCGCTCCACCTCCATCACCACCATCATCGCCATCAAACGACGACGTTCCCTCGTCCAATTCTCTGTCGCAATCGCAGGCGCTTTTGGGAATCCGGAATGATCTGTTGGAGATCGGCGGGAGCTTCAAGACTGGCCTCTCCCTGCTCTCTACGAACAGCAACAAAGCCGTAACCGAGATCTCCAAGTTCGCGTCGAGCTTGCTGCAGCTTCAGAACGAAGCTTTAGAAGAAGGCGGAGCTGCTGATGACGACGGCGATGTTGGTGGAGGTCAAGTGCCGGGGATCACGGACGAAGTTCTCGGTTTCGTTGCCGAGATTTCGGTGTTGCCGGACTGCTGGATCGACTTTCCTATGACGCTCGATCATGATTTCAGCATGACGGATGCTCAGAGAGAGCATGCGTCCGCTGTTGAACAATTGGTTCCGGGATTTGCAGATTTTAGAGTCCGACTTTGCAGTTCCATGAGTGAGGAGCAGTTTTGGATGATCTATTTCTTGTTGCTGCTTCCAAGACTAAATGAACGTGATTTTGAGCTTCTATCAACCCCCAAGATTGTTGAAGCAAGAGATGCACTTCTGCACAAACTTCAAAACAAGACGAACACACAGGAGGATGCCCCAGAGAAGTCAATTCTCGTTTCACCTAAAAGGCACATCCAGGATGGGAAGACACGAGGGGAGGAAGTCTCATCTCAAGAGAAGGAAGCCTTGACTGAGATAGTTAATATTGCAGAAAGGTTAAAGACCGATGACAAAGAGGGCACTGAGCAGTGGTCTGAAGGGGCAAGTATTAGCTCTGGCACTTTAGTGGATGGGCAAAGAAAACATGAACCTGAAGACGATATCTCATTCAGTGATTTAGAGGACGATGAGAATGACCTTTCTAGCAGACAGTCAGGACTAAGGCAAGGACGGGATGCCAGGGCATGTTCACCTAATGGATCAAATGACTGGGTTCAGCTGAACCAAAGCTCGGAAACTGAGGGTGGCCAGAAAAAGGCTGGTCGGTCAAAAGGGAAAGATTCAGAAGGTGAGGATTCAAGTGACTGGTTTGCGGTAGATGAATACGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BSD

Protein Analysis

404

Amino Acids

44.36

Weight (kDa)

4.38

Isoelectric Point (pI)

59.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BSD PF03909 167 - 220 1e-07 BSD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 49, 123, 329
AccIII TCCGGA 1 cut(s) 231
AclWI GGATC 3 cut(s) 428, 485, 1087
AcoI YGGCCR 2 cut(s) 109, 1126
AcuI CTGAAG 3 cut(s) 332, 936, 993
AcyI GRCGYC 1 cut(s) 126
AfiI CCNNNNNNNGG 2 cut(s) 1111, 1135
AgsI TTSAA 5 cut(s) 265, 551, 698, 728, 1179
AjnI CCWGG 2 cut(s) 795, 1058
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AleI CACNNNNGTG 1 cut(s) 944
Alw26I GTCTC 1 cut(s) 829
AlwI GGATC 3 cut(s) 428, 485, 1087
Aor13HI TCCGGA 1 cut(s) 231
AoxI GGCC 3 cut(s) 109, 271, 1126
ApeKI GCWGC 4 cut(s) 340, 343, 377, 640
AspLEI GCGC 1 cut(s) 220
AsuC2I CCSGG 2 cut(s) 417, 564
AsuHPI GGTGA 4 cut(s) 147, 771, 1062, 1181
BaeGI GKGCMC 1 cut(s) 902
BalI TGGCCA 1 cut(s) 1128
BauI CACGAG 1 cut(s) 810
BbsI GAAGAC 2 cut(s) 812, 981
BbvI GCAGC 4 cut(s) 327, 355, 364, 627
BccI CCATC 5 cut(s) 160, 169, 178, 794, 944
BceAI ACGGC 2 cut(s) 290, 406
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BciT130I CCWGG 2 cut(s) 797, 1060
BcnI CCSGG 2 cut(s) 417, 564
BcoDI GTCTC 1 cut(s) 829
BfaI CTAG 1 cut(s) 1023
BfmI CTRYAG 1 cut(s) 341
BfoI RGCGCY 1 cut(s) 221
BglII AGATCT 1 cut(s) 315
BisI GCNGC 6 cut(s) 112, 124, 341, 344, 378, 641
BlsI GCNGC 6 cut(s) 113, 125, 342, 345, 379, 642
Bme1390I CCNGG 4 cut(s) 417, 564, 797, 1060
BmiI GGNNCC 1 cut(s) 561
BmrFI CCNGG 4 cut(s) 417, 564, 797, 1060
BmrI ACTGGG 1 cut(s) 1099
BmsI GCATC 4 cut(s) 511, 697, 742, 1045
BmuI ACTGGG 1 cut(s) 1099
BpiI GAAGAC 2 cut(s) 812, 981
BplI GAGNNNNNCTC 2 cut(s) 809, 841
BpuEI CTTGAG 1 cut(s) 816
BpuMI CCSGG 2 cut(s) 417, 564
BsaHI GRCGYC 1 cut(s) 126
BsaJI CCNNGG 4 cut(s) 106, 121, 416, 1059
BsaWI WCCGGW 1 cut(s) 231
BsaXI ACNNNNNCTCC 2 cut(s) 396, 426
Bsc4I CCNNNNNNNGG 2 cut(s) 1111, 1135
Bse1I ACTGG 3 cut(s) 274, 1094, 1190
BseAI TCCGGA 1 cut(s) 231
BseBI CCWGG 2 cut(s) 797, 1060
BseDI CCNNGG 4 cut(s) 106, 121, 416, 1059
BseGI GGATG 7 cut(s) 526, 627, 757, 792, 805, 955, 1060
BseLI CCNNNNNNNGG 2 cut(s) 1111, 1135
BseMII CTCAG 4 cut(s) 539, 843, 894, 1110
BseNI ACTGG 3 cut(s) 274, 1094, 1190
BseRI GAGGAG 1 cut(s) 623
BseSI GKGCMC 1 cut(s) 902
BseX3I CGGCCG 1 cut(s) 109
BseXI GCAGC 4 cut(s) 327, 355, 364, 627
BsgI GTGCAG 1 cut(s) 701
Bsh1236I CGCG 3 cut(s) 49, 123, 329
Bsh1285I CGRYCG 1 cut(s) 112
BshFI GGCC 3 cut(s) 111, 273, 1128
BsiEI CGRYCG 1 cut(s) 112
BsiSI CCGG 4 cut(s) 232, 416, 467, 563
BslI CCNNNNNNNGG 2 cut(s) 1111, 1135
BsmAI GTCTC 1 cut(s) 829
BsnI GGCC 3 cut(s) 111, 273, 1128
Bsp1286I GDGCHC 1 cut(s) 902
Bsp13I TCCGGA 1 cut(s) 231
Bsp143I GATC 8 cut(s) 238, 249, 315, 420, 477, 499, 624, 1079
BspANI GGCC 3 cut(s) 111, 273, 1128
BspCNI CTCAG 4 cut(s) 538, 844, 895, 1111
BspEI TCCGGA 1 cut(s) 231
BspFNI CGCG 3 cut(s) 49, 123, 329
BspHI TCATGA 1 cut(s) 502
BspLI GGNNCC 1 cut(s) 561
BspMAI CTGCAG 1 cut(s) 345
BspPI GGATC 3 cut(s) 428, 485, 1087
BspQI GCTCTTC 1 cut(s) 17
BsrI ACTGG 3 cut(s) 274, 1094, 1190
BssECI CCNNGG 4 cut(s) 106, 121, 416, 1059
BssMI GATC 8 cut(s) 238, 249, 315, 420, 477, 499, 624, 1079
BssNI GRCGYC 1 cut(s) 126
BssSI CACGAG 1 cut(s) 810
Bst2BI CACGAG 1 cut(s) 810
Bst2UI CCWGG 2 cut(s) 797, 1060
Bst4CI ACNGT 1 cut(s) 1032
Bst6I CTCTTC 1 cut(s) 17
BstACI GRCGYC 1 cut(s) 126
BstAPI GCANNNNNTGC 1 cut(s) 707
BstC8I GCNNGC 3 cut(s) 216, 338, 537
BstDEI CTNAG 5 cut(s) 525, 852, 903, 1040, 1119
BstDSI CCRYGG 1 cut(s) 121
BstF5I GGATG 7 cut(s) 526, 627, 757, 792, 805, 955, 1060
BstFNI CGCG 3 cut(s) 49, 123, 329
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 8 cut(s) 241, 252, 318, 423, 480, 502, 627, 1082
BstMAI GTCTC 1 cut(s) 829
BstMBI GATC 8 cut(s) 238, 249, 315, 420, 477, 499, 624, 1079
BstMCI CGRYCG 1 cut(s) 112
BstMWI GCNNNNNNNGC 3 cut(s) 24, 120, 707
BstNI CCWGG 2 cut(s) 797, 1060
BstNSI RCATGY 2 cut(s) 539, 1068
BstSCI CCNGG 4 cut(s) 415, 562, 795, 1058
BstSFI CTRYAG 1 cut(s) 341
BstSLI GKGCMC 1 cut(s) 902
BstUI CGCG 3 cut(s) 49, 123, 329
BstV1I GCAGC 4 cut(s) 327, 355, 364, 627
BstV2I GAAGAC 2 cut(s) 812, 981
BstX2I RGATCY 1 cut(s) 315
BstYI RGATCY 1 cut(s) 315
BstZI CGGCCG 1 cut(s) 109
BsuRI GGCC 3 cut(s) 111, 273, 1128
BtgI CCRYGG 1 cut(s) 121
BtgZI GCGATG 2 cut(s) 150, 408
BtsCI GGATG 7 cut(s) 526, 627, 757, 792, 805, 955, 1060
BtsI GCAGTG 1 cut(s) 914
BtsIMutI CAGTG 3 cut(s) 900, 914, 997
Cac8I GCNNGC 3 cut(s) 216, 338, 537
CciI TCATGA 1 cut(s) 502
CfoI GCGC 1 cut(s) 220
Cfr42I CCGCGG 1 cut(s) 124
CseI GACGC 4 cut(s) 115, 318, 502, 528
CviAII CATG 6 cut(s) 503, 514, 536, 601, 965, 1065
DdeI CTNAG 5 cut(s) 525, 852, 903, 1040, 1119
DpnI GATC 8 cut(s) 240, 251, 317, 422, 479, 501, 626, 1081
DpnII GATC 8 cut(s) 238, 249, 315, 420, 477, 499, 624, 1079
EaeI YGGCCR 2 cut(s) 109, 1126
EagI CGGCCG 1 cut(s) 109
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
EciI GGCGGA 2 cut(s) 86, 387
EclXI CGGCCG 1 cut(s) 109
Eco32I GATATC 1 cut(s) 982
Eco52I CGGCCG 1 cut(s) 109
Eco57I CTGAAG 3 cut(s) 332, 936, 993
EcoRII CCWGG 2 cut(s) 795, 1058
EcoRV GATATC 1 cut(s) 982
FaeI CATG 6 cut(s) 506, 517, 539, 604, 968, 1068
FaiI YATR 7 cut(s) 491, 504, 515, 537, 602, 966, 1066
FalI AAGNNNNNCTT 2 cut(s) 830, 862
FatI CATG 6 cut(s) 502, 513, 535, 600, 964, 1064
FauI CCCGC 3 cut(s) 32, 54, 248
Fnu4HI GCNGC 6 cut(s) 112, 124, 341, 344, 378, 641
FokI GGATG 7 cut(s) 533, 634, 764, 779, 812, 962, 1067
Fsp4HI GCNGC 6 cut(s) 112, 124, 341, 344, 378, 641
FspBI CTAG 1 cut(s) 1023
GlaI GCGC 1 cut(s) 219
GluI GCNGC 6 cut(s) 112, 124, 341, 344, 378, 641
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 3 cut(s) 111, 273, 1128
HapII CCGG 4 cut(s) 232, 416, 467, 563
HgaI GACGC 4 cut(s) 115, 318, 502, 528
HhaI GCGC 1 cut(s) 220
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 6 cut(s) 506, 517, 539, 604, 968, 1068
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 4 cut(s) 228, 582, 1160, 1175
HpaII CCGG 4 cut(s) 232, 416, 467, 563
HphI GGTGA 4 cut(s) 147, 771, 1062, 1181
Hpy166II GTNNAC 1 cut(s) 1070
Hpy188I TCNGA 6 cut(s) 351, 528, 587, 916, 1114, 1165
Hpy188III TCNNGA 5 cut(s) 232, 265, 503, 833, 1035
Hpy8I GTNNAC 1 cut(s) 1070
Hpy99I CGWCG 8 cut(s) 38, 41, 44, 131, 182, 185, 334, 392
HpyAV CCTTC 5 cut(s) 145, 362, 832, 911, 1160
HpyCH4III ACNGT 1 cut(s) 1032
HpyCH4IV ACGT 2 cut(s) 183, 661
HpyCH4V TGCA 6 cut(s) 343, 572, 594, 710, 718, 869
HpyF10VI GCNNNNNNNGC 3 cut(s) 24, 120, 707
HpyF3I CTNAG 5 cut(s) 525, 852, 903, 1040, 1119
HpySE526I ACGT 2 cut(s) 183, 661
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 6 cut(s) 506, 517, 539, 604, 968, 1068
HspAI GCGC 1 cut(s) 218
Kpn2I TCCGGA 1 cut(s) 231
KspI CCGCGG 1 cut(s) 124
Kzo9I GATC 8 cut(s) 238, 249, 315, 420, 477, 499, 624, 1079
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 4 cut(s) 148, 258, 374, 610
Lsp1109I GCAGC 4 cut(s) 327, 355, 364, 627
LweI GCATC 4 cut(s) 511, 697, 742, 1045
MaeI CTAG 1 cut(s) 1023
MaeII ACGT 2 cut(s) 183, 661
MaeIII GTNAC 2 cut(s) 307, 1181
MalI GATC 8 cut(s) 240, 251, 317, 422, 479, 501, 626, 1081
MboI GATC 8 cut(s) 238, 249, 315, 420, 477, 499, 624, 1079
MboII GAAGA 4 cut(s) 4, 377, 817, 986
MfeI CAATTG 1 cut(s) 554
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 1 cut(s) 902
MlsI TGGCCA 1 cut(s) 1128
MluCI AATT 3 cut(s) 196, 554, 768
MluNI TGGCCA 1 cut(s) 1128
MlyI GAGTC 1 cut(s) 591
MmeI TCCRAC 2 cut(s) 225, 610
Mox20I TGGCCA 1 cut(s) 1128
MroI TCCGGA 1 cut(s) 231
MscI TGGCCA 1 cut(s) 1128
MseI TTAA 2 cut(s) 861, 878
MslI CAYNNNNRTG 1 cut(s) 944
Msp20I TGGCCA 1 cut(s) 1128
MspA1I CMGCKG 3 cut(s) 123, 545, 1099
MspI CCGG 4 cut(s) 232, 416, 467, 563
MspR9I CCNGG 4 cut(s) 417, 564, 797, 1060
MunI CAATTG 1 cut(s) 554
MvaI CCWGG 2 cut(s) 797, 1060
MvnI CGCG 3 cut(s) 49, 123, 329
MwoI GCNNNNNNNGC 3 cut(s) 24, 120, 707
NciI CCSGG 2 cut(s) 417, 564
NdeII GATC 8 cut(s) 238, 249, 315, 420, 477, 499, 624, 1079
NlaIII CATG 6 cut(s) 506, 517, 539, 604, 968, 1068
NlaIV GGNNCC 1 cut(s) 561
NmeAIII GCCGAG 2 cut(s) 87, 475
NmuCI GTSAC 1 cut(s) 1181
NspI RCATGY 2 cut(s) 539, 1068
OliI CACNNNNGTG 1 cut(s) 944
PaeI GCATGC 1 cut(s) 539
PagI TCATGA 1 cut(s) 502
PciSI GCTCTTC 1 cut(s) 17
PfeI GAWTC 3 cut(s) 228, 1160, 1175
PfoI TCCNGGA 2 cut(s) 562, 795
PkrI GCNGC 6 cut(s) 113, 125, 342, 345, 379, 642
PleI GAGTC 1 cut(s) 590
PpsI GAGTC 1 cut(s) 590
Psp6I CCWGG 2 cut(s) 795, 1058
PspGI CCWGG 2 cut(s) 795, 1058
PspN4I GGNNCC 1 cut(s) 561
PstI CTGCAG 1 cut(s) 345
PsuI RGATCY 1 cut(s) 315
PvuII CAGCTG 1 cut(s) 1099
RseI CAYNNNNRTG 1 cut(s) 944
SacII CCGCGG 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 2 cut(s) 861, 878
SatI GCNGC 6 cut(s) 112, 124, 341, 344, 378, 641
Sau3AI GATC 8 cut(s) 238, 249, 315, 420, 477, 499, 624, 1079
SchI GAGTC 1 cut(s) 591
ScrFI CCNGG 4 cut(s) 417, 564, 797, 1060
SduI GDGCHC 1 cut(s) 902
SfaNI GCATC 4 cut(s) 511, 697, 742, 1045
SfcI CTRYAG 1 cut(s) 341
Sfr303I CCGCGG 1 cut(s) 124
SgrBI CCGCGG 1 cut(s) 124
SmiMI CAYNNNNRTG 1 cut(s) 944
SmlI CTYRAG 1 cut(s) 831
SmoI CTYRAG 1 cut(s) 831
SphI GCATGC 1 cut(s) 539
Sse9I AATT 3 cut(s) 196, 554, 768
SspI AATATT 1 cut(s) 865
SspMI CTAG 1 cut(s) 1023
StyD4I CCNGG 4 cut(s) 415, 562, 795, 1058
TaaI ACNGT 1 cut(s) 1032
TaiI ACGT 2 cut(s) 186, 664
TaqI TCGA 3 cut(s) 332, 480, 498
TaqII GACCGA 2 cut(s) 899, 1134
TasI AATT 3 cut(s) 196, 554, 768
TauI GCSGC 2 cut(s) 114, 126
TfiI GAWTC 3 cut(s) 228, 1160, 1175
Tru1I TTAA 2 cut(s) 861, 878
Tru9I TTAA 2 cut(s) 861, 878
TscAI CASTG 3 cut(s) 907, 914, 997
TseFI GTSAC 1 cut(s) 1181
TseI GCWGC 4 cut(s) 340, 343, 377, 640
Tsp45I GTSAC 1 cut(s) 1181
TspDTI ATGAA 2 cut(s) 672, 981
TspGWI ACGGA 2 cut(s) 440, 533
TspRI CASTG 3 cut(s) 907, 914, 997
XceI RCATGY 2 cut(s) 539, 1068
XcmI CCANNNNNNNNNTGG 1 cut(s) 1136
XspI CTAG 1 cut(s) 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.