FvH4_1g04810

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0005364
Physical Location & Seq
Reverse (-)
2542577 .. 2544361
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g04810.t1

Sequence Viewer

Length: 1341 bp
ATGATCCGAACAGGACCACCACTCATCCACTTCCCGAACACCGCGCGTGGACAACCCTCAAAAATATCTAGCCTGACGTGTGATAAAGCGGGGCCATGTGGACCCCGTTATTCAGCCAATGAAATATCAAATAACCTCATCGCCGTATCGCCAGTTGCCACGTTAGCTAGTGATACTGTACACGCGTCTGTTTATATATTACGGTGGTCAGTTTTCCGGGAGAAGAGAGAGAGAGAAAAAAAGGAAAAGGGGATGATGATAACTAACACCTCACAGAATCAGAGAGCTTCTTCTTCCTCTGAAGCAAAGCCGCAGCAGAGTCTGATGATGAGCATGGAGGACAGCGGCAACGTCATCGCGCCTTTCGTTATGAAGACTTACCAGATGGTCAACGATCCCACAACCGACAGGTTAATCTCCTGGGGGAGAGCCAACAACAGCTTCATCGTCGTCGAGCCGCTCGAGTTCTCGCAGCGGCTGTTGCCGGCTTATTTCAAGCACAACAACTTCTCGAGCTTCGTCCGCCAGCTCAACACTTATGGATTCCGGAAAGTTGACCCGGACCGGTGGGAGTTTGCGAACGAGTGTTTCCTGAGAGGGCAGACGCATTTGTTGAAGAACGTGGTGAGGAGAAAACACACGGGCAATCACGGCAACAACAACCACAGGATGGTCAATACGACGGCGTTTGGTAAGGCGGAGGAGCTTGACGACGAGGAGATAGTGATGGAGATAGCGAGGCTGAGGCAGGAGCAGAAGAGCTTAGAGGATGAGATGGAGAGCATGAACAAGAGACTGGAGGCGACGGAGAGAAGACCGCAGCAGATGATGGCGTTTCTTCACAGAGTGGCGGAGGACCCGGAGATTCTGCCACGTATGATGCTGGAGAAGGATAGGAGAATCACGGAGGGGGACAAGAAGCGGCGGCTGATGATTGCCTCGACGTCATCAACTTCGTCGTCGGGGATGGGGGCGACGACAACTTCGGTGAAGACTGAGGAGGAAGTTGACGGGGCCATGGGGGTGCTTTCGTCGCCGGAACCAGGTTTTGAGGTGCAGAGCTTTTGTCGGGGGTCGACGTCGCCGGAGACTTCGACGGCGCAGGCTTGGTGGAGCCAGGGGCGGGTCGTCGGTCGGACTCGGGGTGATCAGGAGTTGTATGGTAGTTCTACGGCCTTAGGCACGCAATCGGGTCATGGAAATGGGAATAGTAGTAATTTCGGATTGGGGTTCGGGTATAGTGGGAATAGGAGTGGTAGTGGAGAAGTGGGTTATTTTGCGGAGGTGGAAGAGAGTCCGCCGCCGCCTTATCCATTCTCCCTGTTAGAAGGTGGGTTTTAG

Protein Analysis

447

Amino Acids

49.73

Weight (kDa)

7.14

Isoelectric Point (pI)

49.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 122 - 211 5.8e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 947, 1082
AccB7I CCANNNNNTGG 1 cut(s) 670
AccBSI CCGCTC 1 cut(s) 460
AccI GTMKAC 1 cut(s) 1076
AccII CGCG 4 cut(s) 44, 46, 185, 359
AccIII TCCGGA 1 cut(s) 546
AclWI GGATC 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 321
AcyI GRCGYC 2 cut(s) 944, 1079
AdeI CACNNNGTG 1 cut(s) 847
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 3 cut(s) 670, 1043, 1123
AflIII ACRYGT 2 cut(s) 77, 183
AgeI ACCGGT 1 cut(s) 564
AgsI TTSAA 2 cut(s) 496, 616
AjiI CACGTC 1 cut(s) 78
AjnI CCWGG 3 cut(s) 419, 1042, 1116
AjuI GAANNNNNNNTTGG 2 cut(s) 425, 457
AluBI AGCT 8 cut(s) 167, 287, 441, 516, 529, 706, 762, 1062
AluI AGCT 8 cut(s) 167, 287, 441, 516, 529, 706, 762, 1062
Alw26I GTCTC 2 cut(s) 787, 1082
AlwI GGATC 1 cut(s) 389
AlwNI CAGNNNCTG 2 cut(s) 322, 478
Ama87I CYCGRG 3 cut(s) 461, 511, 1140
Aor13HI TCCGGA 1 cut(s) 546
AoxI GGCC 3 cut(s) 92, 1014, 1173
ApeKI GCWGC 3 cut(s) 313, 472, 820
ArsI GACNNNNNNTTYG 2 cut(s) 967, 999
AsiGI ACCGGT 1 cut(s) 564
AspLEI GCGC 3 cut(s) 46, 361, 1102
AspS9I GGNCC 6 cut(s) 14, 92, 101, 562, 856, 1014
AsuC2I CCSGG 3 cut(s) 218, 560, 860
AsuHPI GGTGA 3 cut(s) 637, 1000, 1157
AvaI CYCGRG 3 cut(s) 461, 511, 1140
AvaII GGWCC 4 cut(s) 14, 101, 562, 856
AxyI CCTNAGG 1 cut(s) 1177
BbsI GAAGAC 3 cut(s) 380, 820, 998
BbvCI CCTCAGC 1 cut(s) 743
BbvI GCAGC 3 cut(s) 325, 484, 832
BccI CCATC 6 cut(s) 379, 664, 721, 769, 823, 961
BceAI ACGGC 5 cut(s) 128, 667, 699, 1113, 1188
BcgI CGANNNNNNTGC 2 cut(s) 337, 371
BciT130I CCWGG 3 cut(s) 421, 1044, 1118
BclI TGATCA 1 cut(s) 1147
BcnI CCSGG 3 cut(s) 218, 560, 860
BcoDI GTCTC 2 cut(s) 787, 1082
BfaI CTAG 2 cut(s) 69, 168
Bme1390I CCNGG 6 cut(s) 218, 421, 560, 860, 1044, 1118
Bme18I GGWCC 4 cut(s) 14, 101, 562, 856
BmeT110I CYCGRG 3 cut(s) 461, 511, 1140
BmgBI CACGTC 1 cut(s) 78
BmgT120I GGNCC 6 cut(s) 14, 92, 101, 562, 856, 1014
BmiI GGNNCC 6 cut(s) 93, 103, 858, 1015, 1041, 1115
BmrFI CCNGG 6 cut(s) 218, 421, 560, 860, 1044, 1118
BmsI GCATC 1 cut(s) 870
BpiI GAAGAC 3 cut(s) 380, 820, 998
BpmI CTGGAG 2 cut(s) 818, 905
Bpu10I CCTNAGC 1 cut(s) 743
BpuMI CCSGG 3 cut(s) 218, 560, 860
BsaAI YACGTR 1 cut(s) 875
BsaHI GRCGYC 2 cut(s) 944, 1079
BsaJI CCNNGG 3 cut(s) 420, 1017, 1117
BsaWI WCCGGW 2 cut(s) 546, 564
BsaXI ACNNNNNCTCC 2 cut(s) 1243, 1273
Bsc4I CCNNNNNNNGG 3 cut(s) 670, 1043, 1123
Bse118I RCCGGY 2 cut(s) 484, 564
Bse1I ACTGG 2 cut(s) 152, 801
Bse21I CCTNAGG 1 cut(s) 1177
BseAI TCCGGA 1 cut(s) 546
BseBI CCWGG 3 cut(s) 421, 1044, 1118
BseDI CCNNGG 3 cut(s) 420, 1017, 1117
BseGI GGATG 5 cut(s) 24, 258, 675, 775, 972
BseLI CCNNNNNNNGG 3 cut(s) 670, 1043, 1123
BseMII CTCAG 3 cut(s) 584, 734, 987
BseNI ACTGG 2 cut(s) 152, 801
BseRI GAGGAG 4 cut(s) 643, 716, 731, 1013
BseXI GCAGC 3 cut(s) 325, 484, 832
BsgI GTGCAG 1 cut(s) 1076
Bsh1236I CGCG 4 cut(s) 44, 46, 185, 359
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 3 cut(s) 94, 1016, 1175
BshTI ACCGGT 1 cut(s) 564
BsiEI CGRYCG 1 cut(s) 1135
BsiHKCI CYCGRG 3 cut(s) 461, 511, 1140
BsiSI CCGG 8 cut(s) 217, 485, 547, 560, 565, 860, 1037, 1085
BslFI GGGAC 1 cut(s) 926
BslI CCNNNNNNNGG 3 cut(s) 670, 1043, 1123
BsmAI GTCTC 2 cut(s) 787, 1082
BsmFI GGGAC 1 cut(s) 926
BsnI GGCC 3 cut(s) 94, 1016, 1175
BsoBI CYCGRG 3 cut(s) 461, 511, 1140
Bsp13I TCCGGA 1 cut(s) 546
Bsp1407I TGTACA 1 cut(s) 178
Bsp143I GATC 3 cut(s) 3, 394, 1147
Bsp19I CCATGG 1 cut(s) 1017
BspANI GGCC 3 cut(s) 94, 1016, 1175
BspCNI CTCAG 3 cut(s) 585, 735, 988
BspEI TCCGGA 1 cut(s) 546
BspFNI CGCG 4 cut(s) 44, 46, 185, 359
BspLI GGNNCC 6 cut(s) 93, 103, 858, 1015, 1041, 1115
BspPI GGATC 1 cut(s) 389
BspQI GCTCTTC 1 cut(s) 752
BsrBI CCGCTC 1 cut(s) 460
BsrFI RCCGGY 2 cut(s) 484, 564
BsrGI TGTACA 1 cut(s) 178
BsrI ACTGG 2 cut(s) 152, 801
BssAI RCCGGY 2 cut(s) 484, 564
BssECI CCNNGG 3 cut(s) 420, 1017, 1117
BssMI GATC 3 cut(s) 3, 394, 1147
BssNI GRCGYC 2 cut(s) 944, 1079
BssT1I CCWWGG 1 cut(s) 1017
Bst2UI CCWGG 3 cut(s) 421, 1044, 1118
Bst4CI ACNGT 2 cut(s) 178, 204
Bst6I CTCTTC 3 cut(s) 218, 752, 1284
BstACI GRCGYC 2 cut(s) 944, 1079
BstAUI TGTACA 1 cut(s) 178
BstBAI YACGTR 1 cut(s) 875
BstC8I GCNNGC 4 cut(s) 486, 527, 1104, 1184
BstDEI CTNAG 5 cut(s) 593, 743, 763, 996, 1177
BstDSI CCRYGG 1 cut(s) 1017
BstF5I GGATG 5 cut(s) 24, 258, 675, 775, 972
BstFNI CGCG 4 cut(s) 44, 46, 185, 359
BstHHI GCGC 3 cut(s) 46, 361, 1102
BstKTI GATC 3 cut(s) 6, 397, 1150
BstMAI GTCTC 2 cut(s) 787, 1082
BstMBI GATC 3 cut(s) 3, 394, 1147
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 5 cut(s) 164, 481, 522, 651, 1033
BstNI CCWGG 3 cut(s) 421, 1044, 1118
BstSCI CCNGG 6 cut(s) 216, 419, 558, 858, 1042, 1116
BstUI CGCG 4 cut(s) 44, 46, 185, 359
BstV1I GCAGC 3 cut(s) 325, 484, 832
BstV2I GAAGAC 3 cut(s) 380, 820, 998
Bsu36I CCTNAGG 1 cut(s) 1177
BsuRI GGCC 3 cut(s) 94, 1016, 1175
BtgI CCRYGG 1 cut(s) 1017
BtgZI GCGATG 2 cut(s) 124, 340
BtrI CACGTC 1 cut(s) 78
BtsCI GGATG 5 cut(s) 24, 258, 675, 775, 972
Cac8I GCNNGC 4 cut(s) 486, 527, 1104, 1184
CaiI CAGNNNCTG 2 cut(s) 322, 478
CfoI GCGC 3 cut(s) 46, 361, 1102
Cfr10I RCCGGY 2 cut(s) 484, 564
Cfr13I GGNCC 6 cut(s) 14, 92, 101, 562, 856, 1014
CpoI CGGWCCG 1 cut(s) 562
CseI GACGC 2 cut(s) 174, 613
CsiI ACCWGGT 1 cut(s) 1042
Csp6I GTAC 1 cut(s) 179
CspAI ACCGGT 1 cut(s) 564
CspI CGGWCCG 1 cut(s) 562
CviAII CATG 5 cut(s) 96, 334, 784, 1018, 1196
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 5 cut(s) 593, 743, 763, 996, 1177
DpnI GATC 3 cut(s) 5, 396, 1149
DpnII GATC 3 cut(s) 3, 394, 1147
DraIII CACNNNGTG 1 cut(s) 847
Eam1104I CTCTTC 3 cut(s) 218, 752, 1284
EarI CTCTTC 3 cut(s) 218, 752, 1284
EciI GGCGGA 4 cut(s) 512, 713, 866, 1287
Eco130I CCWWGG 1 cut(s) 1017
Eco47I GGWCC 4 cut(s) 14, 101, 562, 856
Eco57I CTGAAG 1 cut(s) 321
Eco81I CCTNAGG 1 cut(s) 1177
Eco88I CYCGRG 3 cut(s) 461, 511, 1140
EcoO109I RGGNCCY 1 cut(s) 856
EcoRII CCWGG 3 cut(s) 419, 1042, 1116
EcoT14I CCWWGG 1 cut(s) 1017
ErhI CCWWGG 1 cut(s) 1017
FaeI CATG 5 cut(s) 99, 337, 787, 1021, 1199
FaqI GGGAC 1 cut(s) 926
FatI CATG 5 cut(s) 95, 333, 783, 1017, 1195
FauI CCCGC 2 cut(s) 82, 1116
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 1076
FokI GGATG 5 cut(s) 11, 265, 682, 782, 979
FspBI CTAG 2 cut(s) 69, 168
GlaI GCGC 3 cut(s) 45, 360, 1101
GsuI CTGGAG 2 cut(s) 818, 905
HaeIII GGCC 3 cut(s) 94, 1016, 1175
HapII CCGG 8 cut(s) 217, 485, 547, 560, 565, 860, 1037, 1085
HgaI GACGC 2 cut(s) 174, 613
HhaI GCGC 3 cut(s) 46, 361, 1102
Hin1I GRCGYC 2 cut(s) 944, 1079
Hin1II CATG 5 cut(s) 99, 337, 787, 1021, 1199
Hin6I GCGC 3 cut(s) 44, 359, 1100
HinP1I GCGC 3 cut(s) 44, 359, 1100
HincII GTYRAC 4 cut(s) 391, 556, 1009, 1077
HindII GTYRAC 4 cut(s) 391, 556, 1009, 1077
HinfI GANTC 7 cut(s) 277, 319, 543, 865, 900, 1138, 1294
HpaII CCGG 8 cut(s) 217, 485, 547, 560, 565, 860, 1037, 1085
HphI GGTGA 3 cut(s) 637, 1000, 1157
Hpy166II GTNNAC 7 cut(s) 50, 101, 181, 391, 556, 1009, 1077
Hpy188I TCNGA 6 cut(s) 8, 282, 301, 324, 1137, 1223
Hpy188III TCNNGA 5 cut(s) 34, 511, 547, 592, 1151
Hpy8I GTNNAC 7 cut(s) 50, 101, 181, 391, 556, 1009, 1077
HpyAV CCTTC 2 cut(s) 883, 1322
HpyCH4III ACNGT 2 cut(s) 178, 204
HpyCH4IV ACGT 7 cut(s) 77, 161, 351, 621, 874, 944, 1079
HpyCH4V TGCA 1 cut(s) 1057
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 481, 522, 651, 1033
HpyF3I CTNAG 5 cut(s) 593, 743, 763, 996, 1177
HpySE526I ACGT 7 cut(s) 77, 161, 351, 621, 874, 944, 1079
Hsp92I GRCGYC 2 cut(s) 944, 1079
Hsp92II CATG 5 cut(s) 99, 337, 787, 1021, 1199
HspAI GCGC 3 cut(s) 44, 359, 1100
Kpn2I TCCGGA 1 cut(s) 546
KroI GCCGGC 1 cut(s) 484
KroNI GCCGGC 1 cut(s) 486
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 3 cut(s) 3, 394, 1147
LguI GCTCTTC 1 cut(s) 752
LmnI GCTCC 3 cut(s) 703, 751, 1113
Lsp1109I GCAGC 3 cut(s) 325, 484, 832
LweI GCATC 1 cut(s) 870
MabI ACCWGGT 1 cut(s) 1042
MaeI CTAG 2 cut(s) 69, 168
MaeII ACGT 7 cut(s) 77, 161, 351, 621, 874, 944, 1079
MalI GATC 3 cut(s) 5, 396, 1149
MbiI CCGCTC 1 cut(s) 460
MboI GATC 3 cut(s) 3, 394, 1147
MluCI AATT 1 cut(s) 1216
MluI ACGCGT 1 cut(s) 183
MlyI GAGTC 3 cut(s) 328, 1132, 1303
MmeI TCCRAC 1 cut(s) 1115
MroI TCCGGA 1 cut(s) 546
MroNI GCCGGC 1 cut(s) 484
MseI TTAA 1 cut(s) 413
MslI CAYNNNNRTG 2 cut(s) 1022, 1200
MspA1I CMGCKG 2 cut(s) 345, 475
MspI CCGG 8 cut(s) 217, 485, 547, 560, 565, 860, 1037, 1085
MspR9I CCNGG 6 cut(s) 218, 421, 560, 860, 1044, 1118
MvaI CCWGG 3 cut(s) 421, 1044, 1118
MvnI CGCG 4 cut(s) 44, 46, 185, 359
MwoI GCNNNNNNNGC 5 cut(s) 164, 481, 522, 651, 1033
NaeI GCCGGC 1 cut(s) 486
NciI CCSGG 3 cut(s) 218, 560, 860
NcoI CCATGG 1 cut(s) 1017
NdeII GATC 3 cut(s) 3, 394, 1147
NgoMIV GCCGGC 1 cut(s) 484
NlaIII CATG 5 cut(s) 99, 337, 787, 1021, 1199
NlaIV GGNNCC 6 cut(s) 93, 103, 858, 1015, 1041, 1115
PaeR7I CTCGAG 2 cut(s) 461, 511
PciSI GCTCTTC 1 cut(s) 752
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 459
PdiI GCCGGC 1 cut(s) 486
PfeI GAWTC 4 cut(s) 277, 543, 865, 900
PflMI CCANNNNNTGG 1 cut(s) 670
PfoI TCCNGGA 1 cut(s) 216
PinAI ACCGGT 1 cut(s) 564
PleI GAGTC 3 cut(s) 327, 1132, 1302
PpsI GAGTC 3 cut(s) 327, 1132, 1302
Ppu21I YACGTR 1 cut(s) 875
PpuMI RGGWCCY 1 cut(s) 856
Psp5II RGGWCCY 1 cut(s) 856
Psp6I CCWGG 3 cut(s) 419, 1042, 1116
PspGI CCWGG 3 cut(s) 419, 1042, 1116
PspN4I GGNNCC 6 cut(s) 93, 103, 858, 1015, 1041, 1115
PspPI GGNCC 6 cut(s) 14, 92, 101, 562, 856, 1014
PspPPI RGGWCCY 1 cut(s) 856
PspXI VCTCGAGB 1 cut(s) 461
PstNI CAGNNNCTG 2 cut(s) 322, 478
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 2 cut(s) 1022, 1200
Rsr2I CGGWCCG 1 cut(s) 562
RsrII CGGWCCG 1 cut(s) 562
SalI GTCGAC 1 cut(s) 1075
SapI GCTCTTC 1 cut(s) 752
SaqAI TTAA 1 cut(s) 413
Sau3AI GATC 3 cut(s) 3, 394, 1147
Sau96I GGNCC 6 cut(s) 14, 92, 101, 562, 856, 1014
SchI GAGTC 3 cut(s) 328, 1132, 1303
ScrFI CCNGG 6 cut(s) 218, 421, 560, 860, 1044, 1118
SexAI ACCWGGT 1 cut(s) 1042
SfaNI GCATC 1 cut(s) 870
Sfr274I CTCGAG 2 cut(s) 461, 511
SinI GGWCC 4 cut(s) 14, 101, 562, 856
SlaI CTCGAG 2 cut(s) 461, 511
SmiMI CAYNNNNRTG 2 cut(s) 1022, 1200
SmlI CTYRAG 2 cut(s) 461, 511
SmoI CTYRAG 2 cut(s) 461, 511
Sse9I AATT 1 cut(s) 1216
SspMI CTAG 2 cut(s) 69, 168
StyD4I CCNGG 6 cut(s) 216, 419, 558, 858, 1042, 1116
StyI CCWWGG 1 cut(s) 1017
TaaI ACNGT 2 cut(s) 178, 204
TaiI ACGT 7 cut(s) 80, 164, 354, 624, 877, 947, 1082
TaqI TCGA 6 cut(s) 453, 462, 512, 941, 1076, 1094
TaqII GACCGA 1 cut(s) 1121
TasI AATT 1 cut(s) 1216
TatI WGTACW 1 cut(s) 178
TauI GCSGC 8 cut(s) 313, 348, 460, 478, 925, 928, 1303, 1306
TfiI GAWTC 4 cut(s) 277, 543, 865, 900
Tru1I TTAA 1 cut(s) 413
Tru9I TTAA 1 cut(s) 413
TseI GCWGC 3 cut(s) 313, 472, 820
TspDTI ATGAA 4 cut(s) 135, 386, 433, 800
TspGWI ACGGA 2 cut(s) 821, 920
Van91I CCANNNNNTGG 1 cut(s) 670
VpaK11BI GGWCC 4 cut(s) 14, 101, 562, 856
XhoI CTCGAG 2 cut(s) 461, 511
XmiI GTMKAC 1 cut(s) 1076
XspI CTAG 2 cut(s) 69, 168
ZraI GACGTC 2 cut(s) 945, 1080
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.