Rh2AG053500

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0005364
Physical Location & Seq
Reverse (-)
4255417 .. 4256566
1150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG053500.1

Sequence Viewer

Length: 1074 bp
ATGATGATAACGAACACATCACACGGAGGAGAGCAGCAGCACCGCCGGTTGGTTCAGAATCCGAGGGCTTCAGAAAAGCCCATGGAGGACAACGGCAACGTCGTCGCGCCGTTCGTTATGAAGACTTATCAGATGGTCAGCGATCCTACAACGGACAGGTTAATCGCTTGGGGGAGGGCTAACAACAGCTTCATCGTCGTCGAGCCTTCGGAGTTCTCGCAGCGGCTGTTGCCGGCTTATTTCAAGCATAATAACTTCTCGAGCTTCGTCCGGCAGCTCAATACTTATGGATTCAGGAAGGTTGATCCGGACAGGTGGGAGTTTGCGAACGAGTGGTTCCTGAGGGGGCAGACGCATTTGTTGAAGAACGTGGTGAGGAGAAAACATAATAATAACAACAGGGCGGTGCTCAACACGACGACGGCGTATGGGAAGTCGGAGGAGCTTGACGACGAAGAAATAGCGACGGAGATAGCGAGGCTGAGGCAGGAGCAGAGGAGCTTAGAGGAAGAGGTGGAGAATATGAGCAAGAGATTGGAGGCGACGGAGAGAAGACCGCAGCAGATGATGGCGTTTCTTCACAGAGTCGCTGAGGATCCCGAGATTTTGCCACGTATGATGCTGGAGAAGGGGTTGGGGGCCAAGAAGCGTCGGCTGGTGATAGCGTCCACGTCTTCCACGTCATCATCGGGGATGGGGGCGACGGCTTCCGTGAAGACTGAGGATGAAGTTGACGGGGCGATGGGCGTGATTTCGTCGCCGGAGACGGGTTTTGAGATGGAGAGCTTTTCTCGGGGGTCCACGTCGCCGGAGGCGTCCACGGCGCAGGGGTGGTGGACCCAGCAGCGGGTTGTGGGTCGGAATCGGAGTGATAAGGAGTTGTATGGTAATTCTACGGGGTTAGCGACGCAGTCGGGTCATGGAAATGGGAATAGTAGTCATTCCGGGTACGGGTTCGGCCCGTTTGGGTATAGTGGAAATAGGAGTGGTAGTGGGGAAGTGGGTTATTTTGCGGAGCTGGAAGAGAGTCCACCACCGCCTTATCCATTCTCACTGTTAGAAGGTGGGTTTTAG

Protein Analysis

357

Amino Acids

39.94

Weight (kDa)

6.13

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 38 - 127 5.2e-29 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 107
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 7 cut(s) 43, 223, 404, 557, 847, 1013, 1037
AclWI GGATC 4 cut(s) 137, 299, 590, 603
AcuI CTGAAG 1 cut(s) 54
AcyI GRCGYC 1 cut(s) 815
AfaI GTAC 1 cut(s) 950
AfiI CCNNNNNNNGG 5 cut(s) 49, 767, 846, 847, 951
AgsI TTSAA 2 cut(s) 244, 364
AjiI CACGTC 3 cut(s) 672, 681, 804
AluBI AGCT 7 cut(s) 189, 264, 277, 445, 501, 786, 1018
AluI AGCT 7 cut(s) 189, 264, 277, 445, 501, 786, 1018
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 1 cut(s) 758
AlwI GGATC 4 cut(s) 137, 299, 590, 603
AlwNI CAGNNNCTG 1 cut(s) 226
Ama87I CYCGRG 3 cut(s) 259, 599, 792
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 2 cut(s) 639, 958
ApeKI GCWGC 6 cut(s) 34, 37, 220, 274, 559, 844
AspLEI GCGC 2 cut(s) 109, 826
AspS9I GGNCC 4 cut(s) 639, 798, 837, 959
AsuC2I CCSGG 1 cut(s) 946
AsuHPI GGTGA 2 cut(s) 385, 670
AvaI CYCGRG 3 cut(s) 259, 599, 792
AvaII GGWCC 2 cut(s) 798, 837
AxyI CCTNAGG 1 cut(s) 341
BamHI GGATCC 1 cut(s) 595
BbsI GAAGAC 4 cut(s) 128, 559, 666, 722
Bbv12I GWGCWC 1 cut(s) 411
BbvCI CCTCAGC 2 cut(s) 482, 591
BbvI GCAGC 6 cut(s) 46, 49, 232, 286, 571, 856
BccI CCATC 5 cut(s) 127, 562, 688, 736, 772
BceAI ACGGC 5 cut(s) 94, 109, 438, 720, 837
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BcnI CCSGG 1 cut(s) 946
BcoDI GTCTC 1 cut(s) 758
BisI GCNGC 7 cut(s) 35, 38, 221, 224, 275, 560, 845
BlsI GCNGC 7 cut(s) 36, 39, 222, 225, 276, 561, 846
Bme1390I CCNGG 1 cut(s) 946
Bme18I GGWCC 2 cut(s) 798, 837
BmeT110I CYCGRG 3 cut(s) 259, 599, 792
BmgBI CACGTC 3 cut(s) 672, 681, 804
BmgT120I GGNCC 4 cut(s) 639, 798, 837, 959
BmiI GGNNCC 5 cut(s) 338, 597, 640, 799, 839
BmrFI CCNGG 1 cut(s) 946
BmsI GCATC 1 cut(s) 609
BpiI GAAGAC 4 cut(s) 128, 559, 666, 722
BplI GAGNNNNNCTC 2 cut(s) 775, 807
BpmI CTGGAG 1 cut(s) 644
Bpu10I CCTNAGC 2 cut(s) 482, 591
BpuMI CCSGG 1 cut(s) 946
BsaAI YACGTR 1 cut(s) 614
BsaHI GRCGYC 1 cut(s) 815
BsaJI CCNNGG 3 cut(s) 62, 81, 819
BsaWI WCCGGW 1 cut(s) 307
BsaXI ACNNNNNCTCC 4 cut(s) 617, 647, 976, 1006
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 767, 846, 847, 951
Bse118I RCCGGY 2 cut(s) 45, 232
Bse21I CCTNAGG 1 cut(s) 341
BseAI TCCGGA 1 cut(s) 307
BseDI CCNNGG 3 cut(s) 62, 81, 819
BseGI GGATG 2 cut(s) 699, 730
BseLI CCNNNNNNNGG 5 cut(s) 49, 767, 846, 847, 951
BseMII CTCAG 4 cut(s) 332, 473, 582, 711
BseRI GAGGAG 4 cut(s) 42, 391, 455, 511
BseXI GCAGC 6 cut(s) 46, 49, 232, 286, 571, 856
BseYI CCCAGC 1 cut(s) 840
Bsh1236I CGCG 1 cut(s) 107
BshFI GGCC 2 cut(s) 641, 960
BsiHKAI GWGCWC 1 cut(s) 411
BsiHKCI CYCGRG 3 cut(s) 259, 599, 792
BsiSI CCGG 7 cut(s) 46, 233, 271, 308, 761, 809, 945
BslI CCNNNNNNNGG 5 cut(s) 49, 767, 846, 847, 951
BsmAI GTCTC 1 cut(s) 758
BsmBI CGTCTC 1 cut(s) 758
BsnI GGCC 2 cut(s) 641, 960
BsoBI CYCGRG 3 cut(s) 259, 599, 792
Bsp1286I GDGCHC 1 cut(s) 411
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 3 cut(s) 142, 304, 595
Bsp19I CCATGG 1 cut(s) 81
BspACI CCGC 7 cut(s) 43, 223, 404, 557, 847, 1013, 1037
BspANI GGCC 2 cut(s) 641, 960
BspCNI CTCAG 4 cut(s) 333, 474, 583, 712
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 5 cut(s) 338, 597, 640, 799, 839
BspPI GGATC 4 cut(s) 137, 299, 590, 603
BsrFI RCCGGY 2 cut(s) 45, 232
BssAI RCCGGY 2 cut(s) 45, 232
BssECI CCNNGG 3 cut(s) 62, 81, 819
BssMI GATC 3 cut(s) 142, 304, 595
BssNI GRCGYC 1 cut(s) 815
BssT1I CCWWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 1056
Bst6I CTCTTC 2 cut(s) 504, 1017
BstACI GRCGYC 1 cut(s) 815
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 5 cut(s) 341, 482, 502, 591, 720
BstDSI CCRYGG 2 cut(s) 81, 819
BstF5I GGATG 2 cut(s) 699, 730
BstFNI CGCG 1 cut(s) 107
BstHHI GCGC 2 cut(s) 109, 826
BstKTI GATC 3 cut(s) 145, 307, 598
BstMAI GTCTC 1 cut(s) 758
BstMBI GATC 3 cut(s) 142, 304, 595
BstMWI GCNNNNNNNGC 2 cut(s) 229, 821
BstSCI CCNGG 1 cut(s) 944
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 6 cut(s) 46, 49, 232, 286, 571, 856
BstV2I GAAGAC 4 cut(s) 128, 559, 666, 722
BstX2I RGATCY 1 cut(s) 595
BstYI RGATCY 1 cut(s) 595
Bsu36I CCTNAGG 1 cut(s) 341
BsuRI GGCC 2 cut(s) 641, 960
BtgI CCRYGG 2 cut(s) 81, 819
BtgZI GCGATG 1 cut(s) 755
BtrI CACGTC 3 cut(s) 672, 681, 804
BtsCI GGATG 2 cut(s) 699, 730
BtsIMutI CAGTG 1 cut(s) 1052
Cac8I GCNNGC 1 cut(s) 234
CaiI CAGNNNCTG 1 cut(s) 226
CfoI GCGC 2 cut(s) 109, 826
Cfr10I RCCGGY 2 cut(s) 45, 232
Cfr13I GGNCC 4 cut(s) 639, 798, 837, 959
CseI GACGC 5 cut(s) 361, 638, 654, 804, 916
Csp6I GTAC 1 cut(s) 949
CviAII CATG 2 cut(s) 82, 920
CviQI GTAC 1 cut(s) 949
DdeI CTNAG 5 cut(s) 341, 482, 502, 591, 720
DpnI GATC 3 cut(s) 144, 306, 597
DpnII GATC 3 cut(s) 142, 304, 595
Eam1104I CTCTTC 2 cut(s) 504, 1017
EarI CTCTTC 2 cut(s) 504, 1017
Eco130I CCWWGG 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 798, 837
Eco57I CTGAAG 1 cut(s) 54
Eco81I CCTNAGG 1 cut(s) 341
Eco88I CYCGRG 3 cut(s) 259, 599, 792
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 758
FaeI CATG 2 cut(s) 85, 923
FatI CATG 2 cut(s) 81, 919
FauI CCCGC 1 cut(s) 840
Fnu4HI GCNGC 7 cut(s) 35, 38, 221, 224, 275, 560, 845
FokI GGATG 2 cut(s) 706, 737
Fsp4HI GCNGC 7 cut(s) 35, 38, 221, 224, 275, 560, 845
GlaI GCGC 2 cut(s) 108, 825
GluI GCNGC 7 cut(s) 35, 38, 221, 224, 275, 560, 845
GsaI CCCAGC 1 cut(s) 844
GsuI CTGGAG 1 cut(s) 644
HaeIII GGCC 2 cut(s) 641, 960
HapII CCGG 7 cut(s) 46, 233, 271, 308, 761, 809, 945
HgaI GACGC 5 cut(s) 361, 638, 654, 804, 916
HhaI GCGC 2 cut(s) 109, 826
Hin1I GRCGYC 1 cut(s) 815
Hin1II CATG 2 cut(s) 85, 923
Hin6I GCGC 2 cut(s) 107, 824
HinP1I GCGC 2 cut(s) 107, 824
HincII GTYRAC 1 cut(s) 733
HindII GTYRAC 1 cut(s) 733
HinfI GANTC 5 cut(s) 58, 291, 585, 862, 1027
HpaII CCGG 7 cut(s) 46, 233, 271, 308, 761, 809, 945
HphI GGTGA 2 cut(s) 385, 670
Hpy166II GTNNAC 6 cut(s) 669, 733, 801, 819, 837, 1031
Hpy188I TCNGA 8 cut(s) 57, 63, 73, 132, 211, 439, 861, 867
Hpy188III TCNNGA 5 cut(s) 259, 295, 308, 340, 599
Hpy8I GTNNAC 6 cut(s) 669, 733, 801, 819, 837, 1031
HpyAV CCTTC 4 cut(s) 216, 292, 622, 1055
HpyCH4III ACNGT 1 cut(s) 1056
HpyCH4IV ACGT 6 cut(s) 99, 369, 613, 671, 680, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 229, 821
HpyF3I CTNAG 5 cut(s) 341, 482, 502, 591, 720
HpySE526I ACGT 6 cut(s) 99, 369, 613, 671, 680, 803
Hsp92I GRCGYC 1 cut(s) 815
Hsp92II CATG 2 cut(s) 85, 923
HspAI GCGC 2 cut(s) 107, 824
Kpn2I TCCGGA 1 cut(s) 307
KroI GCCGGC 1 cut(s) 232
KroNI GCCGGC 1 cut(s) 234
Kzo9I GATC 3 cut(s) 142, 304, 595
LmnI GCTCC 4 cut(s) 442, 490, 498, 1015
Lsp1109I GCAGC 6 cut(s) 46, 49, 232, 286, 571, 856
LweI GCATC 1 cut(s) 609
MaeII ACGT 6 cut(s) 99, 369, 613, 671, 680, 803
MalI GATC 3 cut(s) 144, 306, 597
MboI GATC 3 cut(s) 142, 304, 595
MboII GAAGA 9 cut(s) 133, 376, 467, 521, 564, 569, 666, 727, 1034
MflI RGATCY 1 cut(s) 595
MhlI GDGCHC 1 cut(s) 411
MluCI AATT 1 cut(s) 889
MlyI GAGTC 2 cut(s) 594, 1036
MmeI TCCRAC 2 cut(s) 417, 839
MroI TCCGGA 1 cut(s) 307
MroNI GCCGGC 1 cut(s) 232
MseI TTAA 1 cut(s) 161
MslI CAYNNNNRTG 1 cut(s) 924
MspA1I CMGCKG 2 cut(s) 223, 847
MspI CCGG 7 cut(s) 46, 233, 271, 308, 761, 809, 945
MspR9I CCNGG 1 cut(s) 946
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 2 cut(s) 229, 821
NaeI GCCGGC 1 cut(s) 234
NciI CCSGG 1 cut(s) 946
NcoI CCATGG 1 cut(s) 81
NdeII GATC 3 cut(s) 142, 304, 595
NgoMIV GCCGGC 1 cut(s) 232
NlaIII CATG 2 cut(s) 85, 923
NlaIV GGNNCC 5 cut(s) 338, 597, 640, 799, 839
PaeR7I CTCGAG 1 cut(s) 259
PcsI WCGNNNNNNNCGW 7 cut(s) 99, 111, 422, 473, 677, 812, 902
PdiI GCCGGC 1 cut(s) 234
PfeI GAWTC 3 cut(s) 58, 291, 862
PflFI GACNNNGTC 1 cut(s) 910
PkrI GCNGC 7 cut(s) 36, 39, 222, 225, 276, 561, 846
PleI GAGTC 2 cut(s) 593, 1035
PpsI GAGTC 2 cut(s) 593, 1035
Ppu21I YACGTR 1 cut(s) 614
PspFI CCCAGC 1 cut(s) 840
PspN4I GGNNCC 5 cut(s) 338, 597, 640, 799, 839
PspPI GGNCC 4 cut(s) 639, 798, 837, 959
PstNI CAGNNNCTG 1 cut(s) 226
PsuI RGATCY 1 cut(s) 595
PsyI GACNNNGTC 1 cut(s) 910
RsaI GTAC 1 cut(s) 950
RsaNI GTAC 1 cut(s) 949
RseI CAYNNNNRTG 1 cut(s) 924
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 7 cut(s) 35, 38, 221, 224, 275, 560, 845
Sau3AI GATC 3 cut(s) 142, 304, 595
Sau96I GGNCC 4 cut(s) 639, 798, 837, 959
SchI GAGTC 2 cut(s) 594, 1036
ScrFI CCNGG 1 cut(s) 946
SduI GDGCHC 1 cut(s) 411
SfaNI GCATC 1 cut(s) 609
Sfr274I CTCGAG 1 cut(s) 259
SinI GGWCC 2 cut(s) 798, 837
SlaI CTCGAG 1 cut(s) 259
SmiMI CAYNNNNRTG 1 cut(s) 924
SmlI CTYRAG 1 cut(s) 259
SmoI CTYRAG 1 cut(s) 259
Sse9I AATT 1 cut(s) 889
SsiI CCGC 7 cut(s) 43, 223, 404, 557, 847, 1013, 1037
StyD4I CCNGG 1 cut(s) 944
StyI CCWWGG 1 cut(s) 81
TaaI ACNGT 1 cut(s) 1056
TaiI ACGT 6 cut(s) 102, 372, 616, 674, 683, 806
TaqI TCGA 2 cut(s) 201, 260
TasI AATT 1 cut(s) 889
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 3 cut(s) 58, 291, 862
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TscAI CASTG 1 cut(s) 1059
TseI GCWGC 6 cut(s) 34, 37, 220, 274, 559, 844
TspDTI ATGAA 3 cut(s) 134, 181, 741
TspGWI ACGGA 5 cut(s) 39, 167, 482, 560, 700
TspRI CASTG 1 cut(s) 1059
Tth111I GACNNNGTC 1 cut(s) 910
VpaK11BI GGWCC 2 cut(s) 798, 837
XhoI CTCGAG 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.