pycom02g03920

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0005364
Physical Location & Seq
Reverse (-)
2609327 .. 2610439
1113 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g03920.1

Sequence Viewer

Length: 1014 bp
ATGATGATGGAGGAGGGCAACAACGTCATCGCACCGTTCGTTATGAAGACTTACCAGATGGTCAACGATCCGACGACGGACAATTTAATCACGTGGGGAAGAGCCAACAACAGCTTCGTCGTCGTCGACCCTGTGGTCTTCTCTCAGAGACTGTTACCCGCTTACTTCAAGCACAACAATTTCTCCAGCTTCGTACGCCAGCTCAACACCTATGGATTTCGAAAGGTCGATCCAGATAAGTGGGAGTTCGCGAGCGAGTGGTTTCTGCGAGGGCAGACGCATTTGCTGAGAAACGTGGCGAGGAGAAAACACATGGGCAAGAGTTCGAGTTCGAATTCAAATTCAAACTTCTTACAGGCGAAGCACGAGGATCTCGACGGCGAAGATATAATTAGGGAGATTTCGGGGTTGAAGCAGGAGCAGAAGGCGCTGGAGCAAGAAATCGGGGATATGAACCGGCGGCTGGATGCGACGGAGCGACGGCCGCAGCAAATGATGGCGTTTCTGAACAAAGTGGCGGAGGACCCGGAGATTCTGCCACGTATGATGCTCGAGAAGAACCGCGCGACGGCGGCCCAGTTGGGGGAGAAGAAACGGCGGGTTATGATGATGGCATCGACGTCGTCGTGGTCATCGACAATGGGGGCCACTACTTCCGTCAAAACTGAGGACGAAGATGACGGAACCGTAGGGGTAATTTCCTCATCTCCCGAAGCTGGTTTTGAGATGGAGAGTTTTAATCGGTATCCCACGCCACCGGAGGCCCAGACGGTGTCGGATTGGTTAAGGCAGAGGCGGGTTGTGGATCGGGTTCACGCTGTACAAAACCCGTATAACATGAATACGACGGCGTCGGGTCATGGGGCGGAGAATACCTGGAATAGTAGTAATTCCGCGTATGGGTATGGGAATGGGAGGGGCGGCGTCCCTGGCGGGGAAGTGGGATTCTTTACGGAGGTGGAAGCTAGTCCTCCACCGCCTTATCCATTTTCGTTATTGGAGGGAGGCTTTTAG

Protein Analysis

338

Amino Acids

37.93

Weight (kDa)

5.79

Isoelectric Point (pI)

46.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 13 - 102 4.4e-28 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 134
AatII GACGTC 1 cut(s) 623
AccI GTMKAC 1 cut(s) 126
AccII CGCG 4 cut(s) 251, 564, 566, 896
AclWI GGATC 4 cut(s) 62, 224, 378, 813
AcoI YGGCCR 1 cut(s) 482
AcsI RAATTY 2 cut(s) 334, 340
AcvI CACGTG 1 cut(s) 93
AcyI GRCGYC 3 cut(s) 620, 851, 924
AfaI GTAC 2 cut(s) 195, 822
AfiI CCNNNNNNNGG 7 cut(s) 463, 568, 582, 583, 716, 900, 934
AgsI TTSAA 4 cut(s) 169, 339, 345, 412
AjnI CCWGG 2 cut(s) 875, 928
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 5 cut(s) 114, 189, 202, 716, 965
AluI AGCT 5 cut(s) 114, 189, 202, 716, 965
Alw26I GTCTC 1 cut(s) 142
AlwI GGATC 4 cut(s) 62, 224, 378, 813
AlwNI CAGNNNCTG 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 551
AoxI GGCC 4 cut(s) 482, 573, 645, 762
ApeKI GCWGC 1 cut(s) 487
ApoI RAATTY 2 cut(s) 334, 340
AspLEI GCGC 2 cut(s) 430, 566
AspS9I GGNCC 4 cut(s) 523, 574, 645, 763
AsuC2I CCSGG 1 cut(s) 527
AsuII TTCGAA 2 cut(s) 220, 332
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 1 cut(s) 523
BauI CACGAG 1 cut(s) 365
BbrPI CACGTG 1 cut(s) 93
BbsI GAAGAC 2 cut(s) 53, 130
BbvI GCAGC 1 cut(s) 499
BccI CCATC 4 cut(s) 52, 490, 604, 721
BceAI ACGGC 5 cut(s) 394, 497, 585, 611, 864
BcgI CGANNNNNNTGC 2 cut(s) 603, 637
BciT130I CCWGG 2 cut(s) 877, 930
BciVI GTATCC 1 cut(s) 756
BcnI CCSGG 1 cut(s) 527
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 1 cut(s) 966
BfoI RGCGCY 1 cut(s) 431
BfuI GTATCC 1 cut(s) 756
BisI GCNGC 5 cut(s) 461, 485, 488, 573, 922
BlsI GCNGC 5 cut(s) 462, 486, 489, 574, 923
Bme1390I CCNGG 3 cut(s) 527, 877, 930
Bme18I GGWCC 1 cut(s) 523
BmeT110I CYCGRG 1 cut(s) 551
BmgT120I GGNCC 4 cut(s) 523, 574, 645, 763
BmiI GGNNCC 3 cut(s) 525, 646, 685
BmrFI CCNGG 3 cut(s) 527, 877, 930
BmrI ACTGGG 1 cut(s) 571
BmsI GCATC 3 cut(s) 457, 537, 623
BmuI ACTGGG 1 cut(s) 571
BpiI GAAGAC 2 cut(s) 53, 130
BpmI CTGGAG 2 cut(s) 169, 452
Bpu14I TTCGAA 2 cut(s) 220, 332
BpuMI CCSGG 1 cut(s) 527
BsaAI YACGTR 2 cut(s) 93, 542
BsaHI GRCGYC 3 cut(s) 620, 851, 924
BsaJI CCNNGG 1 cut(s) 928
BsaWI WCCGGW 1 cut(s) 757
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 7 cut(s) 463, 568, 582, 583, 716, 900, 934
Bse118I RCCGGY 1 cut(s) 456
Bse1I ACTGG 1 cut(s) 577
BseBI CCWGG 2 cut(s) 877, 930
BseDI CCNNGG 1 cut(s) 928
BseGI GGATG 1 cut(s) 472
BseLI CCNNNNNNNGG 7 cut(s) 463, 568, 582, 583, 716, 900, 934
BseMII CTCAG 3 cut(s) 158, 278, 657
BseNI ACTGG 1 cut(s) 577
BseRI GAGGAG 2 cut(s) 26, 316
BseX3I CGGCCG 1 cut(s) 482
BseXI GCAGC 1 cut(s) 499
Bsh1236I CGCG 4 cut(s) 251, 564, 566, 896
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 4 cut(s) 484, 575, 647, 764
BsiEI CGRYCG 1 cut(s) 485
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 3 cut(s) 457, 527, 758
BsiWI CGTACG 1 cut(s) 193
BslFI GGGAC 1 cut(s) 911
BslI CCNNNNNNNGG 7 cut(s) 463, 568, 582, 583, 716, 900, 934
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 911
BsnI GGCC 4 cut(s) 484, 575, 647, 764
BsoBI CYCGRG 1 cut(s) 551
Bsp119I TTCGAA 2 cut(s) 220, 332
Bsp1407I TGTACA 1 cut(s) 820
Bsp143I GATC 4 cut(s) 67, 229, 370, 805
Bsp68I TCGCGA 1 cut(s) 251
BspANI GGCC 4 cut(s) 484, 575, 647, 764
BspCNI CTCAG 3 cut(s) 157, 279, 658
BspFNI CGCG 4 cut(s) 251, 564, 566, 896
BspLI GGNNCC 3 cut(s) 525, 646, 685
BspPI GGATC 4 cut(s) 62, 224, 378, 813
BspQI GCTCTTC 1 cut(s) 94
BspT104I TTCGAA 2 cut(s) 220, 332
BsrFI RCCGGY 1 cut(s) 456
BsrGI TGTACA 1 cut(s) 820
BsrI ACTGG 1 cut(s) 577
BssAI RCCGGY 1 cut(s) 456
BssECI CCNNGG 1 cut(s) 928
BssMI GATC 4 cut(s) 67, 229, 370, 805
BssNI GRCGYC 3 cut(s) 620, 851, 924
BssSI CACGAG 1 cut(s) 365
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 2 cut(s) 877, 930
Bst4CI ACNGT 4 cut(s) 36, 153, 688, 772
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 3 cut(s) 620, 851, 924
BstAUI TGTACA 1 cut(s) 820
BstBAI YACGTR 2 cut(s) 93, 542
BstBI TTCGAA 2 cut(s) 220, 332
BstC8I GCNNGC 2 cut(s) 200, 253
BstDEI CTNAG 3 cut(s) 144, 287, 666
BstF5I GGATG 1 cut(s) 472
BstFNI CGCG 4 cut(s) 251, 564, 566, 896
BstH2I RGCGCY 1 cut(s) 431
BstHHI GCGC 2 cut(s) 430, 566
BstKTI GATC 4 cut(s) 70, 232, 373, 808
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 4 cut(s) 67, 229, 370, 805
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 5 cut(s) 195, 427, 484, 572, 930
BstNI CCWGG 2 cut(s) 877, 930
BstSCI CCNGG 3 cut(s) 525, 875, 928
BstUI CGCG 4 cut(s) 251, 564, 566, 896
BstV1I GCAGC 1 cut(s) 499
BstV2I GAAGAC 2 cut(s) 53, 130
BstX2I RGATCY 1 cut(s) 370
BstXI CCANNNNNNTGG 1 cut(s) 240
BstYI RGATCY 1 cut(s) 370
BstZI CGGCCG 1 cut(s) 482
BsuI GTATCC 1 cut(s) 756
BsuRI GGCC 4 cut(s) 484, 575, 647, 764
BtgZI GCGATG 1 cut(s) 13
BtsCI GGATG 1 cut(s) 472
BtuMI TCGCGA 1 cut(s) 251
Cac8I GCNNGC 2 cut(s) 200, 253
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 2 cut(s) 430, 566
Cfr10I RCCGGY 1 cut(s) 456
Cfr13I GGNCC 4 cut(s) 523, 574, 645, 763
CseI GACGC 3 cut(s) 286, 840, 913
Csp6I GTAC 2 cut(s) 194, 821
CviAII CATG 3 cut(s) 313, 838, 860
CviQI GTAC 2 cut(s) 194, 821
DdeI CTNAG 3 cut(s) 144, 287, 666
DpnI GATC 4 cut(s) 69, 231, 372, 807
DpnII GATC 4 cut(s) 67, 229, 370, 805
DrdI GACNNNNNNGTC 1 cut(s) 134
DseDI GACNNNNNNGTC 1 cut(s) 134
EaeI YGGCCR 1 cut(s) 482
EagI CGGCCG 1 cut(s) 482
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
EciI GGCGGA 2 cut(s) 533, 881
EclXI CGGCCG 1 cut(s) 482
Eco47I GGWCC 1 cut(s) 523
Eco52I CGGCCG 1 cut(s) 482
Eco72I CACGTG 1 cut(s) 93
Eco88I CYCGRG 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 523
EcoRI GAATTC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 875, 928
FaeI CATG 3 cut(s) 316, 841, 863
FaqI GGGAC 1 cut(s) 911
FatI CATG 3 cut(s) 312, 837, 859
FauI CCCGC 4 cut(s) 166, 591, 789, 926
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 5 cut(s) 461, 485, 488, 573, 922
FokI GGATG 1 cut(s) 479
Fsp4HI GCNGC 5 cut(s) 461, 485, 488, 573, 922
FspBI CTAG 1 cut(s) 966
GlaI GCGC 2 cut(s) 429, 565
GluI GCNGC 5 cut(s) 461, 485, 488, 573, 922
GsuI CTGGAG 2 cut(s) 169, 452
HaeII RGCGCY 1 cut(s) 431
HaeIII GGCC 4 cut(s) 484, 575, 647, 764
HapII CCGG 3 cut(s) 457, 527, 758
HgaI GACGC 3 cut(s) 286, 840, 913
HhaI GCGC 2 cut(s) 430, 566
Hin1I GRCGYC 3 cut(s) 620, 851, 924
Hin1II CATG 3 cut(s) 316, 841, 863
Hin6I GCGC 2 cut(s) 428, 564
HinP1I GCGC 2 cut(s) 428, 564
HincII GTYRAC 2 cut(s) 64, 127
HindII GTYRAC 2 cut(s) 64, 127
HinfI GANTC 2 cut(s) 532, 945
HpaII CCGG 3 cut(s) 457, 527, 758
Hpy166II GTNNAC 3 cut(s) 64, 127, 814
Hpy188I TCNGA 4 cut(s) 72, 147, 507, 778
Hpy188III TCNNGA 5 cut(s) 233, 250, 374, 553, 710
Hpy8I GTNNAC 3 cut(s) 64, 127, 814
HpyAV CCTTC 1 cut(s) 418
HpyCH4III ACNGT 4 cut(s) 36, 153, 688, 772
HpyCH4IV ACGT 5 cut(s) 24, 92, 294, 541, 620
HpyF10VI GCNNNNNNNGC 5 cut(s) 195, 427, 484, 572, 930
HpyF3I CTNAG 3 cut(s) 144, 287, 666
HpySE526I ACGT 5 cut(s) 24, 92, 294, 541, 620
Hsp92I GRCGYC 3 cut(s) 620, 851, 924
Hsp92II CATG 3 cut(s) 316, 841, 863
HspAI GCGC 2 cut(s) 428, 564
Kzo9I GATC 4 cut(s) 67, 229, 370, 805
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 3 cut(s) 418, 433, 475
Lsp1109I GCAGC 1 cut(s) 499
LweI GCATC 3 cut(s) 457, 537, 623
MaeI CTAG 1 cut(s) 966
MaeII ACGT 5 cut(s) 24, 92, 294, 541, 620
MaeIII GTNAC 1 cut(s) 153
MalI GATC 4 cut(s) 69, 231, 372, 807
MboI GATC 4 cut(s) 67, 229, 370, 805
MboII GAAGA 7 cut(s) 58, 111, 130, 395, 568, 601, 686
MflI RGATCY 1 cut(s) 370
MluCI AATT 7 cut(s) 82, 178, 334, 340, 390, 696, 889
MmeI TCCRAC 2 cut(s) 95, 756
MseI TTAA 3 cut(s) 86, 738, 785
MspI CCGG 3 cut(s) 457, 527, 758
MspR9I CCNGG 3 cut(s) 527, 877, 930
MvaI CCWGG 2 cut(s) 877, 930
MvnI CGCG 4 cut(s) 251, 564, 566, 896
MwoI GCNNNNNNNGC 5 cut(s) 195, 427, 484, 572, 930
NciI CCSGG 1 cut(s) 527
NdeII GATC 4 cut(s) 67, 229, 370, 805
NlaIII CATG 3 cut(s) 316, 841, 863
NlaIV GGNNCC 3 cut(s) 525, 646, 685
NruI TCGCGA 1 cut(s) 251
NspV TTCGAA 2 cut(s) 220, 332
PaeR7I CTCGAG 1 cut(s) 551
PciSI GCTCTTC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 5 cut(s) 36, 123, 372, 623, 632
PfeI GAWTC 2 cut(s) 532, 945
Pfl23II CGTACG 1 cut(s) 193
PflFI GACNNNGTC 3 cut(s) 622, 772, 850
PkrI GCNGC 5 cut(s) 462, 486, 489, 574, 923
PmaCI CACGTG 1 cut(s) 93
PmlI CACGTG 1 cut(s) 93
Ppu21I YACGTR 2 cut(s) 93, 542
PpuMI RGGWCCY 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 875, 928
PspCI CACGTG 1 cut(s) 93
PspGI CCWGG 2 cut(s) 875, 928
PspLI CGTACG 1 cut(s) 193
PspN4I GGNNCC 3 cut(s) 525, 646, 685
PspPI GGNCC 4 cut(s) 523, 574, 645, 763
PspPPI RGGWCCY 1 cut(s) 523
PstNI CAGNNNCTG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 370
PsyI GACNNNGTC 3 cut(s) 622, 772, 850
RruI TCGCGA 1 cut(s) 251
RsaI GTAC 2 cut(s) 195, 822
RsaNI GTAC 2 cut(s) 194, 821
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 3 cut(s) 86, 738, 785
SatI GCNGC 5 cut(s) 461, 485, 488, 573, 922
Sau3AI GATC 4 cut(s) 67, 229, 370, 805
Sau96I GGNCC 4 cut(s) 523, 574, 645, 763
ScrFI CCNGG 3 cut(s) 527, 877, 930
SfaNI GCATC 3 cut(s) 457, 537, 623
Sfr274I CTCGAG 1 cut(s) 551
SfuI TTCGAA 2 cut(s) 220, 332
SinI GGWCC 1 cut(s) 523
SlaI CTCGAG 1 cut(s) 551
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
Sse9I AATT 7 cut(s) 82, 178, 334, 340, 390, 696, 889
SspMI CTAG 1 cut(s) 966
StyD4I CCNGG 3 cut(s) 525, 875, 928
TaaI ACNGT 4 cut(s) 36, 153, 688, 772
TaiI ACGT 5 cut(s) 27, 95, 297, 544, 623
TaqI TCGA 9 cut(s) 126, 220, 228, 326, 332, 375, 552, 617, 635
TasI AATT 7 cut(s) 82, 178, 334, 340, 390, 696, 889
TatI WGTACW 1 cut(s) 820
TauI GCSGC 4 cut(s) 463, 487, 575, 924
TfiI GAWTC 2 cut(s) 532, 945
Tru1I TTAA 3 cut(s) 86, 738, 785
Tru9I TTAA 3 cut(s) 86, 738, 785
TseI GCWGC 1 cut(s) 487
TspDTI ATGAA 3 cut(s) 59, 467, 854
TspGWI ACGGA 5 cut(s) 92, 488, 646, 696, 968
Tth111I GACNNNGTC 3 cut(s) 622, 772, 850
VpaK11BI GGWCC 1 cut(s) 523
XapI RAATTY 2 cut(s) 334, 340
XhoI CTCGAG 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 126
XspI CTAG 1 cut(s) 966
ZraI GACGTC 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.