MD15G1185400.v1.1

Heat Stress Transcription Factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0005364
Physical Location & Seq
Reverse (-)
14671543 .. 14672875
1333 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1185400.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGATGATGGAGGAGAGCAACAACGTCATCGCACCGTTCGTTTTGAAGACTTACCAGATGATTAACGATCCAACGACGGACAATCTAATCACATGGGGAAGAGCCAACAACAGCTTCATCGTCGTCGACCCTCTGGTCTTCTCTCAGAGACTGTTACCCGCTTACTTCAAGCACAACAATTTCTCCAGCTTCGTCCGCCAGCTCAACACCTATGGATTTCGAAAGGTCGATCCAGATAAGTGGGAGTTCGCGAACGAGTGGTTTCTACGAGGACAGACGCATTTGCTGAGAAACGTGGCGAGGAGAAAACAAATGGGCAAGAGTTCTAACTCGAATTCGCTTTTTTCGAATTCGAATTCGAATTCGAATTTCTTACCAACGAAGCACGAGGAGCTCAACGACGAAGATGTAATCATGGAGATTTCGCGGTTGAAGCAGGAGCAGAAGGCGCTGGAGGAAGAAATCGGGAATATGAACCGGCGGCTGGAGACGACGGAGCGACGGCCGCAGCAAATGATGGCGTTTCTACACAAAGTGGCGGAGGACCCGGAGATTCTGCCACGTATGATGCTCGAGAAGGACCGCGCGACGACGGCGCAGTTGGGGGAGAAGAAACGGCGGGTTATGATGATAGCATCGACGTCGTCGTCGTCATCGGGGATGGGGGCCACCACTTCCGTTAGGACTGAGGACGAAGATGATGGAACCGTAGGGGTAATTTCGTCATCTCCCGAAGCAGGTTTTGAGATGGAGAGTTTTAATCAGTATCCCACGTCACCGGAGGTCCAGACGGCGTCGGATTGGTTAAGGCAGATGCGGTTTGTGGATCAAGTTCGCGCTATTCAAAACCCGAATAATATGAATCCGACGGCGTCAGGTCATGGGGCGGAGAATATCCGGAATAGTAGTAGTTCTGGGTACGGGTATGGGAATGGAAATGGTGGCGGCCCTGGCGGGGAAGTGGGATACTTTACGGAGGTGGAAGCTAGTCCGCCACCACCTTATCCATTTTCGCTATTGGAGGGAGGCTTTTAG

Protein Analysis

345

Amino Acids

38.68

Weight (kDa)

5.35

Isoelectric Point (pI)

46.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 13 - 102 8.2e-28 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 134, 646
AatII GACGTC 1 cut(s) 644
Acc36I ACCTGC 1 cut(s) 728
AccI GTMKAC 1 cut(s) 126
AccII CGCG 5 cut(s) 251, 427, 585, 587, 837
AccIII TCCGGA 1 cut(s) 897
AclWI GGATC 3 cut(s) 62, 224, 834
AcoI YGGCCR 1 cut(s) 503
AcsI RAATTY 5 cut(s) 334, 349, 355, 361, 367
AcyI GRCGYC 3 cut(s) 641, 794, 872
AdeI CACNNNGTG 1 cut(s) 535
AfaI GTAC 1 cut(s) 920
AfiI CCNNNNNNNGG 3 cut(s) 484, 737, 955
AgsI TTSAA 4 cut(s) 46, 169, 433, 845
AjiI CACGTC 1 cut(s) 774
AjnI CCWGG 1 cut(s) 949
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 5 cut(s) 114, 189, 202, 394, 986
AluI AGCT 5 cut(s) 114, 189, 202, 394, 986
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 2 cut(s) 142, 482
AlwI GGATC 3 cut(s) 62, 224, 834
AlwNI CAGNNNCTG 1 cut(s) 151
Ama87I CYCGRG 1 cut(s) 572
Aor13HI TCCGGA 1 cut(s) 897
AoxI GGCC 3 cut(s) 503, 666, 946
ApeKI GCWGC 1 cut(s) 508
ApoI RAATTY 5 cut(s) 334, 349, 355, 361, 367
AspLEI GCGC 4 cut(s) 451, 587, 598, 839
AspS9I GGNCC 5 cut(s) 544, 580, 666, 784, 947
AsuC2I CCSGG 1 cut(s) 548
AsuHPI GGTGA 1 cut(s) 768
AsuII TTCGAA 5 cut(s) 220, 347, 353, 359, 365
AvaI CYCGRG 1 cut(s) 572
AvaII GGWCC 3 cut(s) 544, 580, 784
BanII GRGCYC 1 cut(s) 396
BauI CACGAG 1 cut(s) 386
BbsI GAAGAC 2 cut(s) 53, 130
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 1 cut(s) 520
BccI CCATC 4 cut(s) 511, 655, 695, 742
BceAI ACGGC 5 cut(s) 518, 609, 632, 807, 885
BcgI CGANNNNNNTGC 2 cut(s) 624, 658
BciT130I CCWGG 1 cut(s) 951
BciVI GTATCC 2 cut(s) 777, 959
BcnI CCSGG 1 cut(s) 548
BcoDI GTCTC 2 cut(s) 142, 482
BfaI CTAG 1 cut(s) 987
BfoI RGCGCY 1 cut(s) 452
BfuAI ACCTGC 1 cut(s) 728
BfuI GTATCC 2 cut(s) 777, 959
BisI GCNGC 4 cut(s) 482, 506, 509, 946
BlsI GCNGC 4 cut(s) 483, 507, 510, 947
Bme1390I CCNGG 2 cut(s) 548, 951
Bme18I GGWCC 3 cut(s) 544, 580, 784
BmeT110I CYCGRG 1 cut(s) 572
BmgBI CACGTC 1 cut(s) 774
BmgT120I GGNCC 5 cut(s) 544, 580, 666, 784, 947
BmiI GGNNCC 3 cut(s) 546, 667, 706
BmrFI CCNGG 2 cut(s) 548, 951
BmsI GCATC 3 cut(s) 558, 644, 804
BpiI GAAGAC 2 cut(s) 53, 130
BpmI CTGGAG 3 cut(s) 169, 473, 506
Bpu14I TTCGAA 5 cut(s) 220, 347, 353, 359, 365
BpuMI CCSGG 1 cut(s) 548
BsaAI YACGTR 1 cut(s) 563
BsaHI GRCGYC 3 cut(s) 641, 794, 872
BsaJI CCNNGG 1 cut(s) 949
BsaWI WCCGGW 2 cut(s) 778, 897
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 3 cut(s) 484, 737, 955
Bse118I RCCGGY 1 cut(s) 477
BseAI TCCGGA 1 cut(s) 897
BseBI CCWGG 1 cut(s) 951
BseDI CCNNGG 1 cut(s) 949
BseGI GGATG 1 cut(s) 666
BseLI CCNNNNNNNGG 3 cut(s) 484, 737, 955
BseMII CTCAG 3 cut(s) 158, 278, 678
BseRI GAGGAG 3 cut(s) 26, 316, 404
BseX3I CGGCCG 1 cut(s) 503
BseXI GCAGC 1 cut(s) 520
Bsh1236I CGCG 5 cut(s) 251, 427, 585, 587, 837
Bsh1285I CGRYCG 1 cut(s) 506
BshFI GGCC 3 cut(s) 505, 668, 948
BsiEI CGRYCG 1 cut(s) 506
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 572
BsiSI CCGG 4 cut(s) 478, 548, 779, 898
BslI CCNNNNNNNGG 3 cut(s) 484, 737, 955
BsmAI GTCTC 2 cut(s) 142, 482
BsmBI CGTCTC 1 cut(s) 482
BsnI GGCC 3 cut(s) 505, 668, 948
BsoBI CYCGRG 1 cut(s) 572
Bsp119I TTCGAA 5 cut(s) 220, 347, 353, 359, 365
Bsp1286I GDGCHC 1 cut(s) 396
Bsp13I TCCGGA 1 cut(s) 897
Bsp143I GATC 3 cut(s) 67, 229, 826
Bsp68I TCGCGA 1 cut(s) 251
BspANI GGCC 3 cut(s) 505, 668, 948
BspCNI CTCAG 3 cut(s) 157, 279, 679
BspEI TCCGGA 1 cut(s) 897
BspFNI CGCG 5 cut(s) 251, 427, 585, 587, 837
BspLI GGNNCC 3 cut(s) 546, 667, 706
BspMI ACCTGC 1 cut(s) 728
BspPI GGATC 3 cut(s) 62, 224, 834
BspQI GCTCTTC 1 cut(s) 94
BspT104I TTCGAA 5 cut(s) 220, 347, 353, 359, 365
BsrFI RCCGGY 1 cut(s) 477
BssAI RCCGGY 1 cut(s) 477
BssECI CCNNGG 1 cut(s) 949
BssMI GATC 3 cut(s) 67, 229, 826
BssNI GRCGYC 3 cut(s) 641, 794, 872
BssSI CACGAG 1 cut(s) 386
Bst2BI CACGAG 1 cut(s) 386
Bst2UI CCWGG 1 cut(s) 951
Bst4CI ACNGT 3 cut(s) 36, 153, 709
Bst6I CTCTTC 1 cut(s) 94
BstACI GRCGYC 3 cut(s) 641, 794, 872
BstBAI YACGTR 1 cut(s) 563
BstBI TTCGAA 5 cut(s) 220, 347, 353, 359, 365
BstC8I GCNNGC 1 cut(s) 200
BstDEI CTNAG 3 cut(s) 144, 287, 687
BstF5I GGATG 1 cut(s) 666
BstFNI CGCG 5 cut(s) 251, 427, 585, 587, 837
BstH2I RGCGCY 1 cut(s) 452
BstHHI GCGC 4 cut(s) 451, 587, 598, 839
BstKTI GATC 3 cut(s) 70, 232, 829
BstMAI GTCTC 2 cut(s) 142, 482
BstMBI GATC 3 cut(s) 67, 229, 826
BstMCI CGRYCG 1 cut(s) 506
BstMWI GCNNNNNNNGC 7 cut(s) 195, 391, 433, 448, 505, 593, 951
BstNI CCWGG 1 cut(s) 951
BstSCI CCNGG 2 cut(s) 546, 949
BstUI CGCG 5 cut(s) 251, 427, 585, 587, 837
BstV1I GCAGC 1 cut(s) 520
BstV2I GAAGAC 2 cut(s) 53, 130
BstXI CCANNNNNNTGG 1 cut(s) 240
BstZI CGGCCG 1 cut(s) 503
BsuI GTATCC 2 cut(s) 777, 959
BsuRI GGCC 3 cut(s) 505, 668, 948
BtgZI GCGATG 1 cut(s) 13
BtrI CACGTC 1 cut(s) 774
BtsCI GGATG 1 cut(s) 666
BtuMI TCGCGA 1 cut(s) 251
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 1 cut(s) 200
CaiI CAGNNNCTG 1 cut(s) 151
CfoI GCGC 4 cut(s) 451, 587, 598, 839
Cfr10I RCCGGY 1 cut(s) 477
Cfr13I GGNCC 5 cut(s) 544, 580, 666, 784, 947
CseI GACGC 3 cut(s) 286, 783, 861
Csp6I GTAC 1 cut(s) 919
CviAII CATG 3 cut(s) 93, 415, 881
CviQI GTAC 1 cut(s) 919
DdeI CTNAG 3 cut(s) 144, 287, 687
DpnI GATC 3 cut(s) 69, 231, 828
DpnII GATC 3 cut(s) 67, 229, 826
DraIII CACNNNGTG 1 cut(s) 535
DrdI GACNNNNNNGTC 2 cut(s) 134, 646
DseDI GACNNNNNNGTC 2 cut(s) 134, 646
EaeI YGGCCR 1 cut(s) 503
EagI CGGCCG 1 cut(s) 503
Eam1104I CTCTTC 1 cut(s) 94
EarI CTCTTC 1 cut(s) 94
EciI GGCGGA 4 cut(s) 185, 554, 902, 981
Ecl136II GAGCTC 1 cut(s) 394
EclXI CGGCCG 1 cut(s) 503
Eco24I GRGCYC 1 cut(s) 396
Eco47I GGWCC 3 cut(s) 544, 580, 784
Eco52I CGGCCG 1 cut(s) 503
Eco53kI GAGCTC 1 cut(s) 394
Eco88I CYCGRG 1 cut(s) 572
EcoICRI GAGCTC 1 cut(s) 394
EcoO109I RGGNCCY 1 cut(s) 544
EcoRI GAATTC 4 cut(s) 334, 349, 355, 361
EcoRII CCWGG 1 cut(s) 949
EcoT38I GRGCYC 1 cut(s) 396
Esp3I CGTCTC 1 cut(s) 482
FaeI CATG 3 cut(s) 96, 418, 884
FaiI YATR 9 cut(s) 94, 213, 416, 473, 566, 626, 860, 882, 927
FatI CATG 3 cut(s) 92, 414, 880
FauI CCCGC 3 cut(s) 166, 612, 947
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 4 cut(s) 482, 506, 509, 946
FokI GGATG 1 cut(s) 673
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 4 cut(s) 482, 506, 509, 946
FspBI CTAG 1 cut(s) 987
GlaI GCGC 4 cut(s) 450, 586, 597, 838
GluI GCNGC 4 cut(s) 482, 506, 509, 946
GsuI CTGGAG 3 cut(s) 169, 473, 506
HaeII RGCGCY 1 cut(s) 452
HaeIII GGCC 3 cut(s) 505, 668, 948
HapII CCGG 4 cut(s) 478, 548, 779, 898
HgaI GACGC 3 cut(s) 286, 783, 861
HhaI GCGC 4 cut(s) 451, 587, 598, 839
Hin1I GRCGYC 3 cut(s) 641, 794, 872
Hin1II CATG 3 cut(s) 96, 418, 884
Hin6I GCGC 4 cut(s) 449, 585, 596, 837
HinP1I GCGC 4 cut(s) 449, 585, 596, 837
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 2 cut(s) 553, 862
HpaII CCGG 4 cut(s) 478, 548, 779, 898
HphI GGTGA 1 cut(s) 768
Hpy166II GTNNAC 1 cut(s) 127
Hpy188I TCNGA 3 cut(s) 147, 799, 867
Hpy188III TCNNGA 7 cut(s) 233, 250, 466, 574, 731, 787, 898
Hpy8I GTNNAC 1 cut(s) 127
HpyAV CCTTC 2 cut(s) 439, 571
HpyCH4III ACNGT 3 cut(s) 36, 153, 709
HpyCH4IV ACGT 5 cut(s) 24, 294, 562, 641, 773
HpyF10VI GCNNNNNNNGC 7 cut(s) 195, 391, 433, 448, 505, 593, 951
HpyF3I CTNAG 3 cut(s) 144, 287, 687
HpySE526I ACGT 5 cut(s) 24, 294, 562, 641, 773
Hsp92I GRCGYC 3 cut(s) 641, 794, 872
Hsp92II CATG 3 cut(s) 96, 418, 884
HspAI GCGC 4 cut(s) 449, 585, 596, 837
Kpn2I TCCGGA 1 cut(s) 897
Kzo9I GATC 3 cut(s) 67, 229, 826
LguI GCTCTTC 1 cut(s) 94
LmnI GCTCC 3 cut(s) 391, 439, 496
Lsp1109I GCAGC 1 cut(s) 520
LweI GCATC 3 cut(s) 558, 644, 804
MaeI CTAG 1 cut(s) 987
MaeII ACGT 5 cut(s) 24, 294, 562, 641, 773
MaeIII GTNAC 2 cut(s) 153, 774
MalI GATC 3 cut(s) 69, 231, 828
MboI GATC 3 cut(s) 67, 229, 826
MboII GAAGA 7 cut(s) 58, 111, 130, 416, 470, 622, 707
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 7 cut(s) 178, 334, 349, 355, 361, 367, 717
MmeI TCCRAC 3 cut(s) 95, 777, 890
MroI TCCGGA 1 cut(s) 897
MseI TTAA 3 cut(s) 63, 759, 806
MspI CCGG 4 cut(s) 478, 548, 779, 898
MspR9I CCNGG 2 cut(s) 548, 951
MvaI CCWGG 1 cut(s) 951
MvnI CGCG 5 cut(s) 251, 427, 585, 587, 837
MwoI GCNNNNNNNGC 7 cut(s) 195, 391, 433, 448, 505, 593, 951
NciI CCSGG 1 cut(s) 548
NdeII GATC 3 cut(s) 67, 229, 826
NlaIII CATG 3 cut(s) 96, 418, 884
NlaIV GGNNCC 3 cut(s) 546, 667, 706
NmuCI GTSAC 1 cut(s) 774
NruI TCGCGA 1 cut(s) 251
NspV TTCGAA 5 cut(s) 220, 347, 353, 359, 365
PaeR7I CTCGAG 1 cut(s) 572
PciSI GCTCTTC 1 cut(s) 94
PcsI WCGNNNNNNNCGW 4 cut(s) 36, 344, 644, 647
PfeI GAWTC 2 cut(s) 553, 862
PflFI GACNNNGTC 3 cut(s) 643, 793, 871
PkrI GCNGC 4 cut(s) 483, 507, 510, 947
Ppu21I YACGTR 1 cut(s) 563
PpuMI RGGWCCY 1 cut(s) 544
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 544
Psp6I CCWGG 1 cut(s) 949
PspGI CCWGG 1 cut(s) 949
PspN4I GGNNCC 3 cut(s) 546, 667, 706
PspPI GGNCC 5 cut(s) 544, 580, 666, 784, 947
PspPPI RGGWCCY 1 cut(s) 544
PstNI CAGNNNCTG 1 cut(s) 151
PsyI GACNNNGTC 3 cut(s) 643, 793, 871
RruI TCGCGA 1 cut(s) 251
RsaI GTAC 1 cut(s) 920
RsaNI GTAC 1 cut(s) 919
SacI GAGCTC 1 cut(s) 396
SalI GTCGAC 1 cut(s) 125
SapI GCTCTTC 1 cut(s) 94
SaqAI TTAA 3 cut(s) 63, 759, 806
SatI GCNGC 4 cut(s) 482, 506, 509, 946
Sau3AI GATC 3 cut(s) 67, 229, 826
Sau96I GGNCC 5 cut(s) 544, 580, 666, 784, 947
ScrFI CCNGG 2 cut(s) 548, 951
SduI GDGCHC 1 cut(s) 396
SfaNI GCATC 3 cut(s) 558, 644, 804
Sfr274I CTCGAG 1 cut(s) 572
SfuI TTCGAA 5 cut(s) 220, 347, 353, 359, 365
SinI GGWCC 3 cut(s) 544, 580, 784
SlaI CTCGAG 1 cut(s) 572
SmlI CTYRAG 1 cut(s) 572
SmoI CTYRAG 1 cut(s) 572
Sse9I AATT 7 cut(s) 178, 334, 349, 355, 361, 367, 717
SspMI CTAG 1 cut(s) 987
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 546, 949
TaaI ACNGT 3 cut(s) 36, 153, 709
TaiI ACGT 5 cut(s) 27, 297, 565, 644, 776
TasI AATT 7 cut(s) 178, 334, 349, 355, 361, 367, 717
TauI GCSGC 3 cut(s) 484, 508, 948
TfiI GAWTC 2 cut(s) 553, 862
Tru1I TTAA 3 cut(s) 63, 759, 806
Tru9I TTAA 3 cut(s) 63, 759, 806
TseFI GTSAC 1 cut(s) 774
TseI GCWGC 1 cut(s) 508
Tsp45I GTSAC 1 cut(s) 774
TspDTI ATGAA 3 cut(s) 106, 488, 875
TspGWI ACGGA 4 cut(s) 92, 509, 667, 989
Tth111I GACNNNGTC 3 cut(s) 643, 793, 871
VpaK11BI GGWCC 3 cut(s) 544, 580, 784
XapI RAATTY 5 cut(s) 334, 349, 355, 361, 367
XhoI CTCGAG 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 126
XspI CTAG 1 cut(s) 987
ZraI GACGTC 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.