FvH4_1g06300

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0002379
Physical Location & Seq
Forward (+)
3329144 .. 3331608
2465 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06300.t1

Sequence Viewer

Length: 1320 bp
ATGGGTAAAGCTACGAGGTGGCTTAAGGGGTTGTTTGGGATTAAGAGAGACAGAGACCAACACTACAAACCCAGTTCAGATTCAGCTTTGGTGTCCTGTTTTGAGTACAAGAAAGCTTCAAGCTTTGGCAGAGAGGCGAGTCCTCCTCCGGAGACTATGCTTTGTCATAATCCGGCCACAATCCCGCCGAACATTTCACCGTCCGAGGCTGCCTGGCTGAGGTCCTTCTACAATGAAACAGAGAACGAGCAGAGCAAGCACGCAATTGCAGTTGCGGCCGCTACGGCCGCAGCTGCTGATGCCGCGGTTGCGGCGGCACAGGCGGCCGTGGCGGTCGTCCGCCTCACTAGCAATGGCAGGGGGACCATGTTTGGCGGCGGCCGCGAGAGATGGGCTGCCGTCAAGGTCCAAACTTGCTACCGGGGATACTTGGCAAGAAAAGCGCTGAGAGCTCTGAAAGGACTGGTGAAGTTGCAGGCATTAGTGAGAGGCTATTTAGTCAGGAAGCAAGCAACTGCTACACTCCAAGGGATGCAAGCTCTGATCAGAGCACAGGCAACTGTTCGTTCTCATAAATCCCGGGGCCTCAATCTCAACATTAACATCACTGAAGCCAACAGATTCGAAATCCGCGCACGAAAATCCACGGAGAGATTTGATGAGTCTCGGAGTGAGCACACAGCTCCAAGCCATAGCAGGAGGTTGTCTGGTTGTTTAGAAAACAACAGTAGCTTCAGCACCATTGATGAAGTCCCCAAGATTGTTGAGGTTGACACAGGAAGGCCAAAATCAAGGTCTAGGAGAACCAACACTTCCATTTCTGAATTAAGTGACGACCAATTTTACCAGGCAGTGTCTTCTCCACTCCCCTGTTGCTGTCCTGGCAGGTTGATCATCCCGGACCCAAGGAACTTTCAAGATTCCGACTGGGGGCTCACAGGAGAGGAATGCAGGTTCTCCACAGCGCAGAGCACGCCGAGGTTTGTGAACTCCTTCGGGTCTAATGCGCCGGCGACGCCGGCCAAGACTATCTGTGCTGAAAATTTTTACAGGGGATTTGGGAACTATCCCAATTACATGTCGAGCACTCAGTCTTTCAAGGCCAAGTTAAGGTCTCACAGTGCTCCAAAACAGAGGCCGGAGCCGGGGACGAAGAAGAGGCTTTCGCTTAATGAGTTGCTCGAGTCAAGGAGCAGTTTGAGTGGTGTTAAGATGCAGAGGTCTTGTTCACAAGTGCAGGAAGTCATTAATTTCAAGAATGCTGTGATGGGGAAGCTTGATAGGTCATCAGATTTTAGTAGCCTGCTGAGGAGGTCTTGA

Protein Analysis

440

Amino Acids

48.43

Weight (kDa)

10.0

Isoelectric Point (pI)

62.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 334 - 402 5.5e-13 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 876, 942
AccII CGCG 3 cut(s) 305, 384, 633
AccIII TCCGGA 1 cut(s) 148
AcoI YGGCCR 6 cut(s) 174, 276, 285, 324, 379, 1020
AcsI RAATTY 1 cut(s) 1042
AcuI CTGAAG 2 cut(s) 630, 718
AcyI GRCGYC 1 cut(s) 1016
AfaI GTAC 1 cut(s) 107
AfeI AGCGCT 1 cut(s) 444
AfiI CCNNNNNNNGG 4 cut(s) 219, 930, 1110, 1145
AflII CTTAAG 1 cut(s) 23
AflIII ACRYGT 1 cut(s) 1077
AgsI TTSAA 4 cut(s) 120, 917, 1099, 1255
AjnI CCWGG 3 cut(s) 212, 846, 880
AloI GAACNNNNNNTCC 2 cut(s) 938, 970
Alw21I GWGCWC 6 cut(s) 454, 553, 678, 974, 1088, 1126
Alw26I GTCTC 5 cut(s) 42, 48, 146, 669, 1119
AlwNI CAGNNNCTG 1 cut(s) 296
Ama87I CYCGRG 2 cut(s) 579, 1181
Aor13HI TCCGGA 1 cut(s) 148
Aor51HI AGCGCT 1 cut(s) 444
ApeKI GCWGC 4 cut(s) 209, 290, 293, 395
ApoI RAATTY 1 cut(s) 1042
ArsI GACNNNNNNTTYG 2 cut(s) 779, 811
AseI ATTAAT 1 cut(s) 1248
Asp700I GAANNNNTTC 1 cut(s) 992
AspLEI GCGC 4 cut(s) 445, 635, 967, 1009
AspS9I GGNCC 5 cut(s) 222, 363, 406, 583, 901
AsuC2I CCSGG 5 cut(s) 422, 580, 581, 899, 1146
AsuHPI GGTGA 2 cut(s) 189, 478
AsuII TTCGAA 1 cut(s) 624
AvaI CYCGRG 2 cut(s) 579, 1181
AvaII GGWCC 4 cut(s) 222, 363, 406, 901
BanII GRGCYC 2 cut(s) 454, 936
BbsI GAAGAC 1 cut(s) 849
Bbv12I GWGCWC 6 cut(s) 454, 553, 678, 974, 1088, 1126
BbvCI CCTCAGC 2 cut(s) 218, 1307
BbvI GCAGC 4 cut(s) 196, 280, 302, 382
BccI CCATC 2 cut(s) 384, 1261
BceAI ACGGC 3 cut(s) 300, 311, 383
BciT130I CCWGG 3 cut(s) 214, 848, 882
BciVI GTATCC 1 cut(s) 419
BclI TGATCA 2 cut(s) 543, 891
BcnI CCSGG 5 cut(s) 422, 580, 581, 899, 1146
BcoDI GTCTC 5 cut(s) 42, 48, 146, 669, 1119
BfaI CTAG 2 cut(s) 348, 798
BfoI RGCGCY 1 cut(s) 446
BfrI CTTAAG 1 cut(s) 23
BfuAI ACCTGC 2 cut(s) 876, 942
BfuI GTATCC 1 cut(s) 419
BglI GCCNNNNNGGC 1 cut(s) 284
Bme1390I CCNGG 8 cut(s) 214, 422, 580, 581, 848, 882, 899, 1146
Bme18I GGWCC 4 cut(s) 222, 363, 406, 901
BmeT110I CYCGRG 2 cut(s) 579, 1181
BmgT120I GGNCC 5 cut(s) 222, 363, 406, 583, 901
BmiI GGNNCC 4 cut(s) 364, 584, 903, 1143
BmrFI CCNGG 8 cut(s) 214, 422, 580, 581, 848, 882, 899, 1146
BmrI ACTGGG 2 cut(s) 66, 937
BmsI GCATC 3 cut(s) 289, 522, 1203
BmuI ACTGGG 2 cut(s) 66, 937
BpiI GAAGAC 1 cut(s) 849
BplI GAGNNNNNCTC 2 cut(s) 130, 162
Bpu10I CCTNAGC 2 cut(s) 218, 1307
Bpu14I TTCGAA 1 cut(s) 624
BpuMI CCSGG 5 cut(s) 422, 580, 581, 899, 1146
BsaHI GRCGYC 1 cut(s) 1016
BsaI GGTCTC 2 cut(s) 48, 1119
BsaWI WCCGGW 1 cut(s) 148
Bsc4I CCNNNNNNNGG 4 cut(s) 219, 930, 1110, 1145
Bse118I RCCGGY 2 cut(s) 1009, 1018
Bse1I ACTGG 3 cut(s) 72, 468, 932
Bse3DI GCAATG 1 cut(s) 358
BseAI TCCGGA 1 cut(s) 148
BseBI CCWGG 3 cut(s) 214, 848, 882
BseGI GGATG 2 cut(s) 537, 894
BseLI CCNNNNNNNGG 4 cut(s) 219, 930, 1110, 1145
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 4 cut(s) 209, 437, 1103, 1298
BseNI ACTGG 3 cut(s) 72, 468, 932
BseRI GAGGAG 1 cut(s) 135
BseX3I CGGCCG 4 cut(s) 276, 285, 324, 379
BseXI GCAGC 4 cut(s) 196, 280, 302, 382
BsgI GTGCAG 1 cut(s) 1256
Bsh1236I CGCG 3 cut(s) 305, 384, 633
Bsh1285I CGRYCG 5 cut(s) 279, 288, 327, 336, 382
BsiEI CGRYCG 5 cut(s) 279, 288, 327, 336, 382
BsiHKAI GWGCWC 6 cut(s) 454, 553, 678, 974, 1088, 1126
BsiHKCI CYCGRG 2 cut(s) 579, 1181
BsiSI CCGG 9 cut(s) 149, 173, 421, 580, 899, 1010, 1019, 1139, 1145
BslFI GGGAC 3 cut(s) 376, 737, 1162
BslI CCNNNNNNNGG 4 cut(s) 219, 930, 1110, 1145
BsmAI GTCTC 5 cut(s) 42, 48, 146, 669, 1119
BsmFI GGGAC 3 cut(s) 376, 737, 1162
BsmI GAATGC 2 cut(s) 953, 1264
Bso31I GGTCTC 2 cut(s) 48, 1119
BsoBI CYCGRG 2 cut(s) 579, 1181
Bsp119I TTCGAA 1 cut(s) 624
Bsp1286I GDGCHC 7 cut(s) 454, 553, 678, 936, 974, 1088, 1126
Bsp13I TCCGGA 1 cut(s) 148
Bsp143I GATC 2 cut(s) 543, 891
BspCNI CTCAG 4 cut(s) 210, 438, 1102, 1299
BspEI TCCGGA 1 cut(s) 148
BspFNI CGCG 3 cut(s) 305, 384, 633
BspLI GGNNCC 4 cut(s) 364, 584, 903, 1143
BspMI ACCTGC 2 cut(s) 876, 942
BspT104I TTCGAA 1 cut(s) 624
BspTI CTTAAG 1 cut(s) 23
BspTNI GGTCTC 2 cut(s) 48, 1119
BsrDI GCAATG 1 cut(s) 358
BsrFI RCCGGY 2 cut(s) 1009, 1018
BsrI ACTGG 3 cut(s) 72, 468, 932
BssAI RCCGGY 2 cut(s) 1009, 1018
BssMI GATC 2 cut(s) 543, 891
BssNI GRCGYC 1 cut(s) 1016
BssT1I CCWWGG 2 cut(s) 526, 905
Bst2UI CCWGG 3 cut(s) 214, 848, 882
Bst4CI ACNGT 4 cut(s) 201, 562, 728, 1121
Bst6I CTCTTC 1 cut(s) 1151
BstACI GRCGYC 1 cut(s) 1016
BstAFI CTTAAG 1 cut(s) 23
BstBI TTCGAA 1 cut(s) 624
BstC8I GCNNGC 9 cut(s) 257, 261, 477, 510, 537, 974, 1011, 1020, 1304
BstDEI CTNAG 4 cut(s) 218, 446, 1089, 1307
BstDSI CCRYGG 3 cut(s) 303, 327, 645
BstENI CCTNNNNNAGG 1 cut(s) 217
BstF5I GGATG 2 cut(s) 537, 894
BstFNI CGCG 3 cut(s) 305, 384, 633
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 4 cut(s) 445, 635, 967, 1009
BstKTI GATC 2 cut(s) 546, 894
BstMAI GTCTC 5 cut(s) 42, 48, 146, 669, 1119
BstMBI GATC 2 cut(s) 543, 891
BstMCI CGRYCG 5 cut(s) 279, 288, 327, 336, 382
BstNI CCWGG 3 cut(s) 214, 848, 882
BstNSI RCATGY 1 cut(s) 1081
BstSCI CCNGG 8 cut(s) 212, 420, 578, 579, 846, 880, 897, 1144
BstUI CGCG 3 cut(s) 305, 384, 633
BstV1I GCAGC 4 cut(s) 196, 280, 302, 382
BstV2I GAAGAC 1 cut(s) 849
BstZI CGGCCG 4 cut(s) 276, 285, 324, 379
BsuI GTATCC 1 cut(s) 419
BtgI CCRYGG 3 cut(s) 303, 327, 645
BtsCI GGATG 2 cut(s) 537, 894
BtsI GCAGTG 1 cut(s) 858
BtsIMutI CAGTG 3 cut(s) 606, 858, 1126
BveI ACCTGC 2 cut(s) 876, 942
Cac8I GCNNGC 9 cut(s) 257, 261, 477, 510, 537, 974, 1011, 1020, 1304
CaiI CAGNNNCTG 1 cut(s) 296
CciNI GCGGCCGC 2 cut(s) 276, 379
CfoI GCGC 4 cut(s) 445, 635, 967, 1009
Cfr10I RCCGGY 2 cut(s) 1009, 1018
Cfr13I GGNCC 5 cut(s) 222, 363, 406, 583, 901
Cfr42I CCGCGG 1 cut(s) 306
Cfr9I CCCGGG 1 cut(s) 579
CseI GACGC 1 cut(s) 1024
Csp6I GTAC 1 cut(s) 106
CviAII CATG 2 cut(s) 367, 1078
CviQI GTAC 1 cut(s) 106
DdeI CTNAG 4 cut(s) 218, 446, 1089, 1307
DpnI GATC 2 cut(s) 545, 893
DpnII GATC 2 cut(s) 543, 891
EaeI YGGCCR 6 cut(s) 174, 276, 285, 324, 379, 1020
EagI CGGCCG 4 cut(s) 276, 285, 324, 379
Eam1104I CTCTTC 1 cut(s) 1151
EarI CTCTTC 1 cut(s) 1151
EciI GGCGGA 1 cut(s) 329
Ecl136II GAGCTC 1 cut(s) 452
EclXI CGGCCG 4 cut(s) 276, 285, 324, 379
Eco130I CCWWGG 2 cut(s) 526, 905
Eco24I GRGCYC 2 cut(s) 454, 936
Eco31I GGTCTC 2 cut(s) 48, 1119
Eco47I GGWCC 4 cut(s) 222, 363, 406, 901
Eco47III AGCGCT 1 cut(s) 444
Eco52I CGGCCG 4 cut(s) 276, 285, 324, 379
Eco53kI GAGCTC 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 630, 718
Eco88I CYCGRG 2 cut(s) 579, 1181
EcoICRI GAGCTC 1 cut(s) 452
EcoNI CCTNNNNNAGG 1 cut(s) 217
EcoO109I RGGNCCY 2 cut(s) 222, 583
EcoRII CCWGG 3 cut(s) 212, 846, 880
EcoT14I CCWWGG 2 cut(s) 526, 905
EcoT38I GRGCYC 2 cut(s) 454, 936
ErhI CCWWGG 2 cut(s) 526, 905
FaeI CATG 2 cut(s) 370, 1081
FaiI YATR 6 cut(s) 158, 168, 368, 573, 693, 1079
FaqI GGGAC 3 cut(s) 376, 737, 1162
FatI CATG 2 cut(s) 366, 1077
FauI CCCGC 1 cut(s) 192
FbaI TGATCA 2 cut(s) 543, 891
FokI GGATG 2 cut(s) 544, 881
FriOI GRGCYC 2 cut(s) 454, 936
FspBI CTAG 2 cut(s) 348, 798
GlaI GCGC 4 cut(s) 444, 634, 966, 1008
HaeII RGCGCY 1 cut(s) 446
HapII CCGG 9 cut(s) 149, 173, 421, 580, 899, 1010, 1019, 1139, 1145
HgaI GACGC 1 cut(s) 1024
HhaI GCGC 4 cut(s) 445, 635, 967, 1009
Hin1I GRCGYC 1 cut(s) 1016
Hin1II CATG 2 cut(s) 370, 1081
Hin6I GCGC 4 cut(s) 443, 633, 965, 1007
HinP1I GCGC 4 cut(s) 443, 633, 965, 1007
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HindIII AAGCTT 3 cut(s) 114, 121, 1274
HinfI GANTC 6 cut(s) 80, 139, 621, 662, 920, 1184
HpaII CCGG 9 cut(s) 149, 173, 421, 580, 899, 1010, 1019, 1139, 1145
HphI GGTGA 2 cut(s) 189, 478
Hpy166II GTNNAC 3 cut(s) 772, 988, 1229
Hpy188I TCNGA 9 cut(s) 79, 205, 456, 543, 548, 669, 823, 925, 1291
Hpy188III TCNNGA 5 cut(s) 149, 502, 917, 1255, 1317
Hpy8I GTNNAC 3 cut(s) 772, 988, 1229
Hpy99I CGWCG 1 cut(s) 1018
HpyAV CCTTC 3 cut(s) 235, 774, 1003
HpyCH4III ACNGT 4 cut(s) 201, 562, 728, 1121
HpyCH4V TGCA 6 cut(s) 269, 475, 535, 951, 1216, 1237
HpyF3I CTNAG 4 cut(s) 218, 446, 1089, 1307
Hsp92I GRCGYC 1 cut(s) 1016
Hsp92II CATG 2 cut(s) 370, 1081
HspAI GCGC 4 cut(s) 443, 633, 965, 1007
Kpn2I TCCGGA 1 cut(s) 148
KroI GCCGGC 2 cut(s) 1009, 1018
KroNI GCCGGC 2 cut(s) 1011, 1020
Ksp22I TGATCA 2 cut(s) 543, 891
KspI CCGCGG 1 cut(s) 306
Kzo9I GATC 2 cut(s) 543, 891
LmnI GCTCC 4 cut(s) 688, 1129, 1141, 1191
Lsp1109I GCAGC 4 cut(s) 196, 280, 302, 382
LweI GCATC 3 cut(s) 289, 522, 1203
MaeI CTAG 2 cut(s) 348, 798
MaeIII GTNAC 1 cut(s) 830
MalI GATC 2 cut(s) 545, 893
MboI GATC 2 cut(s) 543, 891
MboII GAAGA 3 cut(s) 849, 1165, 1168
MfeI CAATTG 1 cut(s) 264
MhlI GDGCHC 7 cut(s) 454, 553, 678, 936, 974, 1088, 1126
MluCI AATT 6 cut(s) 264, 824, 839, 1042, 1072, 1249
MlyI GAGTC 3 cut(s) 148, 671, 1193
MmeI TCCRAC 1 cut(s) 948
MreI CGCCGGCG 1 cut(s) 1009
MroI TCCGGA 1 cut(s) 148
MroNI GCCGGC 2 cut(s) 1009, 1018
MroXI GAANNNNTTC 1 cut(s) 992
MseI TTAA 8 cut(s) 24, 42, 600, 827, 1109, 1170, 1209, 1248
MspA1I CMGCKG 2 cut(s) 293, 305
MspCI CTTAAG 1 cut(s) 23
MspI CCGG 9 cut(s) 149, 173, 421, 580, 899, 1010, 1019, 1139, 1145
MspR9I CCNGG 8 cut(s) 214, 422, 580, 581, 848, 882, 899, 1146
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 2 cut(s) 953, 1264
MvaI CCWGG 3 cut(s) 214, 848, 882
MvnI CGCG 3 cut(s) 305, 384, 633
NaeI GCCGGC 2 cut(s) 1011, 1020
NciI CCSGG 5 cut(s) 422, 580, 581, 899, 1146
NdeII GATC 2 cut(s) 543, 891
NgoMIV GCCGGC 2 cut(s) 1009, 1018
NlaIII CATG 2 cut(s) 370, 1081
NlaIV GGNNCC 4 cut(s) 364, 584, 903, 1143
NmeAIII GCCGAG 1 cut(s) 1002
NmuCI GTSAC 1 cut(s) 830
NotI GCGGCCGC 2 cut(s) 276, 379
NspI RCATGY 1 cut(s) 1081
NspV TTCGAA 1 cut(s) 624
PaeR7I CTCGAG 1 cut(s) 1181
PciI ACATGT 1 cut(s) 1077
PctI GAATGC 2 cut(s) 953, 1264
PdiI GCCGGC 2 cut(s) 1011, 1020
PdmI GAANNNNTTC 1 cut(s) 992
PfeI GAWTC 3 cut(s) 80, 621, 920
PfoI TCCNGGA 1 cut(s) 897
PleI GAGTC 3 cut(s) 147, 670, 1192
PpsI GAGTC 3 cut(s) 147, 670, 1192
PpuMI RGGWCCY 1 cut(s) 222
PscI ACATGT 1 cut(s) 1077
PshBI ATTAAT 1 cut(s) 1248
Psp124BI GAGCTC 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 222
Psp6I CCWGG 3 cut(s) 212, 846, 880
PspGI CCWGG 3 cut(s) 212, 846, 880
PspN4I GGNNCC 4 cut(s) 364, 584, 903, 1143
PspPI GGNCC 5 cut(s) 222, 363, 406, 583, 901
PspPPI RGGWCCY 1 cut(s) 222
PspXI VCTCGAGB 1 cut(s) 1181
PstNI CAGNNNCTG 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 293
RsaI GTAC 1 cut(s) 107
RsaNI GTAC 1 cut(s) 106
SacI GAGCTC 1 cut(s) 454
SacII CCGCGG 1 cut(s) 306
SaqAI TTAA 8 cut(s) 24, 42, 600, 827, 1109, 1170, 1209, 1248
Sau3AI GATC 2 cut(s) 543, 891
Sau96I GGNCC 5 cut(s) 222, 363, 406, 583, 901
SchI GAGTC 3 cut(s) 148, 671, 1193
ScrFI CCNGG 8 cut(s) 214, 422, 580, 581, 848, 882, 899, 1146
SduI GDGCHC 7 cut(s) 454, 553, 678, 936, 974, 1088, 1126
SfaNI GCATC 3 cut(s) 289, 522, 1203
SfiI GGCCNNNNNGGCC 1 cut(s) 284
Sfr274I CTCGAG 1 cut(s) 1181
Sfr303I CCGCGG 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 624
SgrAI CRCCGGYG 1 cut(s) 1009
SgrBI CCGCGG 1 cut(s) 306
SinI GGWCC 4 cut(s) 222, 363, 406, 901
SlaI CTCGAG 1 cut(s) 1181
SmaI CCCGGG 1 cut(s) 581
SmlI CTYRAG 2 cut(s) 23, 1181
SmoI CTYRAG 2 cut(s) 23, 1181
Sse9I AATT 6 cut(s) 264, 824, 839, 1042, 1072, 1249
SspMI CTAG 2 cut(s) 348, 798
SstI GAGCTC 1 cut(s) 454
StyD4I CCNGG 8 cut(s) 212, 420, 578, 579, 846, 880, 897, 1144
StyI CCWWGG 2 cut(s) 526, 905
TaaI ACNGT 4 cut(s) 201, 562, 728, 1121
TaqI TCGA 3 cut(s) 624, 1082, 1182
TasI AATT 6 cut(s) 264, 824, 839, 1042, 1072, 1249
TatI WGTACW 1 cut(s) 105
TfiI GAWTC 3 cut(s) 80, 621, 920
Tru1I TTAA 8 cut(s) 24, 42, 600, 827, 1109, 1170, 1209, 1248
Tru9I TTAA 8 cut(s) 24, 42, 600, 827, 1109, 1170, 1209, 1248
TscAI CASTG 3 cut(s) 613, 858, 1126
TseFI GTSAC 1 cut(s) 830
TseI GCWGC 4 cut(s) 209, 290, 293, 395
Tsp45I GTSAC 1 cut(s) 830
TspDTI ATGAA 2 cut(s) 249, 762
TspGWI ACGGA 1 cut(s) 662
TspMI CCCGGG 1 cut(s) 579
TspRI CASTG 3 cut(s) 613, 858, 1126
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 4 cut(s) 222, 363, 406, 901
VspI ATTAAT 1 cut(s) 1248
XagI CCTNNNNNAGG 1 cut(s) 217
XapI RAATTY 1 cut(s) 1042
XceI RCATGY 1 cut(s) 1081
XhoI CTCGAG 1 cut(s) 1181
XmaI CCCGGG 1 cut(s) 579
XmnI GAANNNNTTC 1 cut(s) 992
XspI CTAG 2 cut(s) 348, 798
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.