Rw2G005310

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0002379
Physical Location & Seq
Reverse (-)
5032823 .. 5034012
1190 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005310.1

Sequence Viewer

Length: 951 bp
ATGTTCGGCGGCGGCCGCGAGAGATGGGCAGCCGTCAAGGTCCAAACTTGCTTCCGGGCATACTTGGCCAGAAAAGCCCTGAGAGCTCTGAAAGGACTGGTGAAGTTACAGGCATTAGTGAGAGGCTATTTAGTGAGGAAGCAAGCCACTGCTACACTCCACGGTATGCAAGCTCTGGTCAGAGCACAGGCCACTGTTCGTTCTCATAAATCCAGGGGCCTCAACCTCAACATTAACATCACTGAAGCCAACAGAATCGAAATGCGCGCACGAAAATCCACGGAGAGGTTTGATGAGAGTCGGAGCGAGCACACAGCTCCGAGCCACAGCAGGAGGCTCTCTGGTTCTTTGGAGAACAACAACAGTTTCAACACCATTGATGAAATTCCCAAGATTGTTGAGGTTGATACAGGAAGGCCCAAATCCAGGTCTCGCAGAACCAACACTTCCATTTCTGAACTAAGCGACGAATTTTACCAGGCAGTGTCTTCTCCGCTCCCCTGTTGCTGTCCGGCCAGGTTGACCATCCCCGACCACCGGAACTTCCAAGAGTCCGACTGGGGGCTCACCGGAGAGGAATGCAGGTTCTCCACGGCGCAGAGCACGCCGAGATTTGTGAACTCGTGCGGGTCTAATGCCCCGGCGACGCCGGCCAAGAGTGTCTGCGCCGACAACTTTTACAGGGGATTTGGGAACTACCCGAACTACATGGCGAGCACTCAGTCTTTCAAGGCCAAGTTGAGGTCTCACAGCGCTCCGAAACAGAGGCCGGAGCCGGGGACGAAGAAGAGGCTTTCGCTTAATGAGTTGCTCGAGTCAAGGAACAGTTTGAGTGGTGTTAAGATGCAGAGGTCTTGTTCACAAGTGCAGGAAGCCATTAATTTCAAGAATGCTGTGATGGGGAAGCTTGATAGGTCGTCAGATTTTAGTAGCTTGCTGAGGAGGTCTTAA

Protein Analysis

316

Amino Acids

35.35

Weight (kDa)

10.28

Isoelectric Point (pI)

58.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 204 - 280 1.7e-14 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 573
AccBSI CCGCTC 1 cut(s) 496
AccII CGCG 2 cut(s) 18, 267
AciI CCGC 5 cut(s) 9, 12, 16, 494, 627
AcoI YGGCCR 4 cut(s) 13, 66, 513, 651
AcsI RAATTY 2 cut(s) 384, 470
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 647
AfeI AGCGCT 1 cut(s) 754
AfiI CCNNNNNNNGG 6 cut(s) 285, 426, 537, 561, 741, 776
AgsI TTSAA 3 cut(s) 370, 730, 886
AjnI CCWGG 4 cut(s) 212, 425, 477, 515
AloI GAACNNNNNNTCC 2 cut(s) 569, 601
AluBI AGCT 5 cut(s) 86, 173, 317, 907, 933
AluI AGCT 5 cut(s) 86, 173, 317, 907, 933
Alw21I GWGCWC 5 cut(s) 88, 187, 312, 605, 719
Alw26I GTCTC 2 cut(s) 435, 750
Ama87I CYCGRG 1 cut(s) 812
Aor51HI AGCGCT 1 cut(s) 754
AoxI GGCC 9 cut(s) 13, 66, 189, 217, 416, 513, 651, 732, 767
ApeKI GCWGC 1 cut(s) 29
ApoI RAATTY 2 cut(s) 384, 470
AseI ATTAAT 1 cut(s) 879
AspLEI GCGC 5 cut(s) 267, 269, 598, 668, 755
AspS9I GGNCC 3 cut(s) 40, 217, 417
AsuC2I CCSGG 3 cut(s) 56, 641, 777
AsuHPI GGTGA 2 cut(s) 112, 559
AvaI CYCGRG 1 cut(s) 812
AvaII GGWCC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 68
BanII GRGCYC 2 cut(s) 88, 567
BauI CACGAG 1 cut(s) 622
BbsI GAAGAC 1 cut(s) 480
Bbv12I GWGCWC 5 cut(s) 88, 187, 312, 605, 719
BbvCI CCTCAGC 1 cut(s) 938
BbvI GCAGC 1 cut(s) 41
BccI CCATC 3 cut(s) 18, 533, 892
BceAI ACGGC 2 cut(s) 17, 609
BciT130I CCWGG 4 cut(s) 214, 427, 479, 517
BcnI CCSGG 3 cut(s) 56, 641, 777
BcoDI GTCTC 2 cut(s) 435, 750
BfoI RGCGCY 1 cut(s) 756
BfuAI ACCTGC 1 cut(s) 573
BisI GCNGC 4 cut(s) 10, 13, 16, 30
BlsI GCNGC 4 cut(s) 11, 14, 17, 31
Bme1390I CCNGG 7 cut(s) 56, 214, 427, 479, 517, 641, 777
Bme18I GGWCC 1 cut(s) 40
BmeT110I CYCGRG 1 cut(s) 812
BmgT120I GGNCC 3 cut(s) 40, 217, 417
BmiI GGNNCC 2 cut(s) 218, 774
BmrFI CCNGG 7 cut(s) 56, 214, 427, 479, 517, 641, 777
BmrI ACTGGG 1 cut(s) 568
BmsI GCATC 1 cut(s) 834
BmuI ACTGGG 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 480
Bpu10I CCTNAGC 1 cut(s) 938
BpuMI CCSGG 3 cut(s) 56, 641, 777
BsaHI GRCGYC 1 cut(s) 647
BsaI GGTCTC 2 cut(s) 435, 750
BsaJI CCNNGG 6 cut(s) 160, 213, 279, 591, 639, 776
BsaWI WCCGGW 2 cut(s) 537, 569
Bsc4I CCNNNNNNNGG 6 cut(s) 285, 426, 537, 561, 741, 776
Bse118I RCCGGY 1 cut(s) 649
Bse1I ACTGG 2 cut(s) 102, 563
BseBI CCWGG 4 cut(s) 214, 427, 479, 517
BseDI CCNNGG 6 cut(s) 160, 213, 279, 591, 639, 776
BseGI GGATG 1 cut(s) 525
BseLI CCNNNNNNNGG 6 cut(s) 285, 426, 537, 561, 741, 776
BseMII CTCAG 3 cut(s) 71, 734, 929
BseNI ACTGG 2 cut(s) 102, 563
BsePI GCGCGC 1 cut(s) 265
BseX3I CGGCCG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 41
BsgI GTGCAG 1 cut(s) 887
Bsh1236I CGCG 2 cut(s) 18, 267
Bsh1285I CGRYCG 1 cut(s) 16
BshFI GGCC 9 cut(s) 15, 68, 191, 219, 418, 515, 653, 734, 769
BsiEI CGRYCG 1 cut(s) 16
BsiHKAI GWGCWC 5 cut(s) 88, 187, 312, 605, 719
BsiHKCI CYCGRG 1 cut(s) 812
BsiSI CCGG 8 cut(s) 55, 512, 538, 570, 641, 650, 770, 776
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 6 cut(s) 285, 426, 537, 561, 741, 776
BsmAI GTCTC 2 cut(s) 435, 750
BsmFI GGGAC 1 cut(s) 793
BsmI GAATGC 2 cut(s) 584, 895
BsnI GGCC 9 cut(s) 15, 68, 191, 219, 418, 515, 653, 734, 769
Bso31I GGTCTC 2 cut(s) 435, 750
BsoBI CYCGRG 1 cut(s) 812
Bsp1286I GDGCHC 6 cut(s) 88, 187, 312, 567, 605, 719
BspACI CCGC 5 cut(s) 9, 12, 16, 494, 627
BspANI GGCC 9 cut(s) 15, 68, 191, 219, 418, 515, 653, 734, 769
BspCNI CTCAG 3 cut(s) 72, 733, 930
BspFNI CGCG 2 cut(s) 18, 267
BspLI GGNNCC 2 cut(s) 218, 774
BspMI ACCTGC 1 cut(s) 573
BspTNI GGTCTC 2 cut(s) 435, 750
BsrBI CCGCTC 1 cut(s) 496
BsrFI RCCGGY 1 cut(s) 649
BsrI ACTGG 2 cut(s) 102, 563
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 6 cut(s) 160, 213, 279, 591, 639, 776
BssHII GCGCGC 1 cut(s) 265
BssNI GRCGYC 1 cut(s) 647
BssSI CACGAG 1 cut(s) 622
Bst2BI CACGAG 1 cut(s) 622
Bst2UI CCWGG 4 cut(s) 214, 427, 479, 517
Bst4CI ACNGT 4 cut(s) 164, 196, 365, 827
Bst6I CTCTTC 1 cut(s) 782
BstACI GRCGYC 1 cut(s) 647
BstC8I GCNNGC 8 cut(s) 144, 171, 267, 308, 605, 651, 715, 935
BstDEI CTNAG 4 cut(s) 80, 461, 720, 938
BstDSI CCRYGG 3 cut(s) 160, 279, 591
BstF5I GGATG 1 cut(s) 525
BstFNI CGCG 2 cut(s) 18, 267
BstH2I RGCGCY 1 cut(s) 756
BstHHI GCGC 5 cut(s) 267, 269, 598, 668, 755
BstMAI GTCTC 2 cut(s) 435, 750
BstMCI CGRYCG 1 cut(s) 16
BstMWI GCNNNNNNNGC 6 cut(s) 15, 65, 74, 83, 604, 650
BstNI CCWGG 4 cut(s) 214, 427, 479, 517
BstSCI CCNGG 7 cut(s) 54, 212, 425, 477, 515, 639, 775
BstUI CGCG 2 cut(s) 18, 267
BstV1I GCAGC 1 cut(s) 41
BstV2I GAAGAC 1 cut(s) 480
BstZI CGGCCG 1 cut(s) 13
BsuRI GGCC 9 cut(s) 15, 68, 191, 219, 418, 515, 653, 734, 769
BtgI CCRYGG 3 cut(s) 160, 279, 591
BtsCI GGATG 1 cut(s) 525
BtsI GCAGTG 2 cut(s) 147, 489
BtsIMutI CAGTG 4 cut(s) 147, 192, 240, 489
BveI ACCTGC 1 cut(s) 573
Cac8I GCNNGC 8 cut(s) 144, 171, 267, 308, 605, 651, 715, 935
CciNI GCGGCCGC 1 cut(s) 13
CfoI GCGC 5 cut(s) 267, 269, 598, 668, 755
Cfr10I RCCGGY 1 cut(s) 649
Cfr13I GGNCC 3 cut(s) 40, 217, 417
CseI GACGC 1 cut(s) 655
CviAII CATG 1 cut(s) 709
DdeI CTNAG 4 cut(s) 80, 461, 720, 938
EaeI YGGCCR 4 cut(s) 13, 66, 513, 651
EagI CGGCCG 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 782
EarI CTCTTC 1 cut(s) 782
Ecl136II GAGCTC 1 cut(s) 86
EclXI CGGCCG 1 cut(s) 13
Eco24I GRGCYC 2 cut(s) 88, 567
Eco31I GGTCTC 2 cut(s) 435, 750
Eco47I GGWCC 1 cut(s) 40
Eco47III AGCGCT 1 cut(s) 754
Eco52I CGGCCG 1 cut(s) 13
Eco53kI GAGCTC 1 cut(s) 86
Eco57I CTGAAG 1 cut(s) 264
Eco88I CYCGRG 1 cut(s) 812
EcoICRI GAGCTC 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 217
EcoRII CCWGG 4 cut(s) 212, 425, 477, 515
EcoT38I GRGCYC 2 cut(s) 88, 567
FaeI CATG 1 cut(s) 712
FaiI YATR 4 cut(s) 61, 167, 207, 710
FaqI GGGAC 1 cut(s) 793
FatI CATG 1 cut(s) 708
FauI CCCGC 1 cut(s) 620
Fnu4HI GCNGC 4 cut(s) 10, 13, 16, 30
FokI GGATG 1 cut(s) 512
FriOI GRGCYC 2 cut(s) 88, 567
Fsp4HI GCNGC 4 cut(s) 10, 13, 16, 30
GlaI GCGC 5 cut(s) 266, 268, 597, 667, 754
GluI GCNGC 4 cut(s) 10, 13, 16, 30
HaeII RGCGCY 1 cut(s) 756
HaeIII GGCC 9 cut(s) 15, 68, 191, 219, 418, 515, 653, 734, 769
HapII CCGG 8 cut(s) 55, 512, 538, 570, 641, 650, 770, 776
HgaI GACGC 1 cut(s) 655
HhaI GCGC 5 cut(s) 267, 269, 598, 668, 755
Hin1I GRCGYC 1 cut(s) 647
Hin1II CATG 1 cut(s) 712
Hin6I GCGC 5 cut(s) 265, 267, 596, 666, 753
HinP1I GCGC 5 cut(s) 265, 267, 596, 666, 753
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HindIII AAGCTT 1 cut(s) 905
HinfI GANTC 4 cut(s) 255, 298, 551, 815
HpaII CCGG 8 cut(s) 55, 512, 538, 570, 641, 650, 770, 776
HphI GGTGA 2 cut(s) 112, 559
Hpy166II GTNNAC 3 cut(s) 522, 619, 860
Hpy188I TCNGA 8 cut(s) 90, 182, 303, 321, 457, 556, 759, 922
Hpy188III TCNNGA 1 cut(s) 886
Hpy8I GTNNAC 3 cut(s) 522, 619, 860
Hpy99I CGWCG 2 cut(s) 470, 649
HpyAV CCTTC 1 cut(s) 408
HpyCH4III ACNGT 4 cut(s) 164, 196, 365, 827
HpyCH4V TGCA 4 cut(s) 169, 582, 847, 868
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 65, 74, 83, 604, 650
HpyF3I CTNAG 4 cut(s) 80, 461, 720, 938
Hsp92I GRCGYC 1 cut(s) 647
Hsp92II CATG 1 cut(s) 712
HspAI GCGC 5 cut(s) 265, 267, 596, 666, 753
KroI GCCGGC 1 cut(s) 649
KroNI GCCGGC 1 cut(s) 651
LmnI GCTCC 5 cut(s) 303, 322, 501, 760, 772
Lsp1109I GCAGC 1 cut(s) 41
LweI GCATC 1 cut(s) 834
MaeIII GTNAC 1 cut(s) 105
MbiI CCGCTC 1 cut(s) 496
MboII GAAGA 3 cut(s) 480, 796, 799
MhlI GDGCHC 6 cut(s) 88, 187, 312, 567, 605, 719
MlsI TGGCCA 1 cut(s) 68
MluCI AATT 3 cut(s) 384, 470, 880
MluNI TGGCCA 1 cut(s) 68
MlyI GAGTC 3 cut(s) 307, 560, 824
MmeI TCCRAC 2 cut(s) 281, 579
Mox20I TGGCCA 1 cut(s) 68
MroNI GCCGGC 1 cut(s) 649
MscI TGGCCA 1 cut(s) 68
MseI TTAA 5 cut(s) 234, 801, 840, 879, 949
Msp20I TGGCCA 1 cut(s) 68
MspI CCGG 8 cut(s) 55, 512, 538, 570, 641, 650, 770, 776
MspR9I CCNGG 7 cut(s) 56, 214, 427, 479, 517, 641, 777
Mva1269I GAATGC 2 cut(s) 584, 895
MvaI CCWGG 4 cut(s) 214, 427, 479, 517
MvnI CGCG 2 cut(s) 18, 267
MwoI GCNNNNNNNGC 6 cut(s) 15, 65, 74, 83, 604, 650
NaeI GCCGGC 1 cut(s) 651
NciI CCSGG 3 cut(s) 56, 641, 777
NgoMIV GCCGGC 1 cut(s) 649
NlaIII CATG 1 cut(s) 712
NlaIV GGNNCC 2 cut(s) 218, 774
NmeAIII GCCGAG 1 cut(s) 633
NotI GCGGCCGC 1 cut(s) 13
PaeR7I CTCGAG 1 cut(s) 812
PauI GCGCGC 1 cut(s) 265
PctI GAATGC 2 cut(s) 584, 895
PdiI GCCGGC 1 cut(s) 651
PfeI GAWTC 1 cut(s) 255
PkrI GCNGC 4 cut(s) 11, 14, 17, 31
PleI GAGTC 3 cut(s) 306, 559, 823
PpsI GAGTC 3 cut(s) 306, 559, 823
PshBI ATTAAT 1 cut(s) 879
Psp124BI GAGCTC 1 cut(s) 88
Psp6I CCWGG 4 cut(s) 212, 425, 477, 515
PspGI CCWGG 4 cut(s) 212, 425, 477, 515
PspN4I GGNNCC 2 cut(s) 218, 774
PspPI GGNCC 3 cut(s) 40, 217, 417
PspXI VCTCGAGB 1 cut(s) 812
PteI GCGCGC 1 cut(s) 265
SacI GAGCTC 1 cut(s) 88
SaqAI TTAA 5 cut(s) 234, 801, 840, 879, 949
SatI GCNGC 4 cut(s) 10, 13, 16, 30
Sau96I GGNCC 3 cut(s) 40, 217, 417
SchI GAGTC 3 cut(s) 307, 560, 824
ScrFI CCNGG 7 cut(s) 56, 214, 427, 479, 517, 641, 777
SduI GDGCHC 6 cut(s) 88, 187, 312, 567, 605, 719
SfaNI GCATC 1 cut(s) 834
Sfr274I CTCGAG 1 cut(s) 812
SinI GGWCC 1 cut(s) 40
SlaI CTCGAG 1 cut(s) 812
SmlI CTYRAG 1 cut(s) 812
SmoI CTYRAG 1 cut(s) 812
Sse9I AATT 3 cut(s) 384, 470, 880
SsiI CCGC 5 cut(s) 9, 12, 16, 494, 627
SstI GAGCTC 1 cut(s) 88
StyD4I CCNGG 7 cut(s) 54, 212, 425, 477, 515, 639, 775
TaaI ACNGT 4 cut(s) 164, 196, 365, 827
TaqI TCGA 2 cut(s) 258, 813
TasI AATT 3 cut(s) 384, 470, 880
TauI GCSGC 3 cut(s) 12, 15, 18
TfiI GAWTC 1 cut(s) 255
Tru1I TTAA 5 cut(s) 234, 801, 840, 879, 949
Tru9I TTAA 5 cut(s) 234, 801, 840, 879, 949
TscAI CASTG 4 cut(s) 154, 199, 247, 489
TseI GCWGC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 396
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 4 cut(s) 154, 199, 247, 489
VpaK11BI GGWCC 1 cut(s) 40
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 2 cut(s) 384, 470
XhoI CTCGAG 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.