Rh2BG426000

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002379
Physical Location & Seq
Forward (+)
60070277 .. 60073626
3350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG426000.1

Sequence Viewer

Length: 1242 bp
ATGGGGAGAGCTAGAAGATGGTTGAAGGCTCTGTTGGGGATGAAGAAAGAGAAAGACCCTCTTGAGCTTCCAAATAGTACTACCAATACTACTCGGCCTGGTGATCGGAAAGAGAAGAGAAGGTGGAGTTTCAGCAAGTCATTGAAAGATGCAAGTCCAATTAGTCATGTCCCGGCTAATCTTCCCCCGAATGTTTCCCCTGCTGACTCAGCCTGGCTCAGATCCTATCTTGCCGAGACAGAGAAGGAACAGAACAAGCACGCAATTGCGGTGGCTGCTGCCACTGCCGCCGCTGCTGACGCTGCCGTGGCTGCTGCTCAGGCGGCGGTGGCGGTTGTGAGGCTGACGAGCAAGGGCGGCTGTGGAGGAGACTTGTTTGGTAAGAGAGAGAGATGGGCGGCAATGAGGATTCAGACTGTTTTCAGAGGCTATTTGGCAAGGAAGGCGCACCGAGCTCTTAGAGGACTTGTTAAGTTGCAGGCTCTTGTTAGAGGTTTTCTTGTCAGGAAACGAGCTGCTGCTACTCTTCATGGTATGCAAGCTCTTATTAGAGCACAGACAAGTGTCCGCTACCAGCGAGCACGTCGTTCTTTCAATAAAGAGAACAGGTTCCTACCCGAAAGCAGGCCTAGGAAGTCCATTCACTCCATTGAGAGATTTGATGAAGCAAGAAGTGAATTTTATAGCAAGAGGCTTTCTGCAACTTATGAACCTTCTCTTAATGGCTTGGATGAAAGCCCAAAGATTGTTGAAATCGATACCTTCAGAACCAGATCCAGGTCTCGCCGATACAACACTGTCTTATCTGAATATGGCGAAGACGTGCCGCCTTACACCTCTTCGCCACTGCCATGTCCAATTCCAACTCGCATTACTGTACCGGGGGGCCAGCAGCTTCAGGATTGTGAATGGTACTTCACTGCTGATGAATGCAAGTTCACTACAGCCCATAGTACCCCCCGTTTGGCCAGTTCTTTTCGGTCTAATGCACCCAGTACACCTGCCAAGAGTGTTTGTGGAGACAGCTTCTTTCAGCCTTACTCAAACTGCCCAAACTATATGTCTAGCACCAAGTCATTTAAGGCCAAATCAAGGTCCCACAGTGCACCGAAGCAAAGGCCTGAACCCCTGCCTAGGAAGAGGCTTTCCCTTGACGAAATATTGGCAGCGAGAAACAGTGTGAGTGGCATTCGAATGCATCGACCCTACAACCAAGTCCAAGAAGAAGTCTTGAATTATTGA

Protein Analysis

413

Amino Acids

46.36

Weight (kDa)

10.32

Isoelectric Point (pI)

63.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 317 - 395 3.1e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1009
Acc36I ACCTGC 1 cut(s) 1009
AclWI GGATC 2 cut(s) 216, 768
AcoI YGGCCR 1 cut(s) 966
AcsI RAATTY 1 cut(s) 677
AcuI CTGAAG 2 cut(s) 748, 881
AfaI GTAC 5 cut(s) 79, 879, 914, 955, 997
AfiI CCNNNNNNNGG 5 cut(s) 624, 777, 964, 1092, 1134
AgsI TTSAA 5 cut(s) 25, 145, 595, 752, 1234
AjiI CACGTC 2 cut(s) 584, 823
AjnI CCWGG 3 cut(s) 97, 212, 776
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 7 cut(s) 11, 67, 455, 515, 542, 895, 1026
AluI AGCT 7 cut(s) 11, 67, 455, 515, 542, 895, 1026
Alw21I GWGCWC 4 cut(s) 457, 556, 583, 1108
Alw26I GTCTC 4 cut(s) 230, 363, 786, 1014
Alw44I GTGCAC 1 cut(s) 1104
AlwI GGATC 2 cut(s) 216, 768
AoxI GGCC 6 cut(s) 95, 626, 886, 966, 1083, 1118
ApaLI GTGCAC 1 cut(s) 1104
ApoI RAATTY 1 cut(s) 677
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
Asp700I GAANNNNTTC 1 cut(s) 608
AspA2I CCTAGG 2 cut(s) 629, 1133
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 2 cut(s) 886, 1095
AsuC2I CCSGG 2 cut(s) 173, 882
AsuHPI GGTGA 1 cut(s) 113
AsuII TTCGAA 1 cut(s) 1192
AvaII GGWCC 1 cut(s) 1095
AvrII CCTAGG 2 cut(s) 629, 1133
BaeGI GKGCMC 1 cut(s) 1108
BalI TGGCCA 1 cut(s) 968
BanII GRGCYC 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 825
Bbv12I GWGCWC 4 cut(s) 457, 556, 583, 1108
BccI CCATC 2 cut(s) 12, 387
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 3 cut(s) 99, 214, 778
BcnI CCSGG 2 cut(s) 173, 882
BcoDI GTCTC 4 cut(s) 230, 363, 786, 1014
BfaI CTAG 4 cut(s) 12, 630, 1065, 1134
BfmI CTRYAG 1 cut(s) 942
BfuAI ACCTGC 1 cut(s) 1009
BlnI CCTAGG 2 cut(s) 629, 1133
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 5 cut(s) 99, 173, 214, 778, 882
Bme18I GGWCC 1 cut(s) 1095
BmgBI CACGTC 2 cut(s) 584, 823
BmgT120I GGNCC 2 cut(s) 886, 1095
BmiI GGNNCC 3 cut(s) 611, 887, 1097
BmrFI CCNGG 5 cut(s) 99, 173, 214, 778, 882
BmrI ACTGGG 1 cut(s) 987
BmsI GCATC 2 cut(s) 139, 1207
BmuI ACTGGG 1 cut(s) 987
BoxI GACNNNNGTC 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 825
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 1192
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 173, 882
Bsa29I ATCGAT 1 cut(s) 756
BsaI GGTCTC 1 cut(s) 786
BsaJI CCNNGG 4 cut(s) 306, 629, 881, 1133
Bsc4I CCNNNNNNNGG 5 cut(s) 624, 777, 964, 1092, 1134
Bse1I ACTGG 2 cut(s) 969, 993
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 3 cut(s) 99, 214, 778
BseCI ATCGAT 1 cut(s) 756
BseDI CCNNGG 4 cut(s) 306, 629, 881, 1133
BseGI GGATG 2 cut(s) 45, 736
BseLI CCNNNNNNNGG 5 cut(s) 624, 777, 964, 1092, 1134
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 3 cut(s) 222, 232, 332
BseNI ACTGG 2 cut(s) 969, 993
BseRI GAGGAG 1 cut(s) 381
BseSI GKGCMC 1 cut(s) 1108
BshFI GGCC 6 cut(s) 97, 628, 888, 968, 1085, 1120
BshVI ATCGAT 1 cut(s) 756
BsiHKAI GWGCWC 4 cut(s) 457, 556, 583, 1108
BsiSI CCGG 2 cut(s) 173, 881
BslFI GGGAC 2 cut(s) 155, 1081
BslI CCNNNNNNNGG 5 cut(s) 624, 777, 964, 1092, 1134
BsmAI GTCTC 4 cut(s) 230, 363, 786, 1014
BsmFI GGGAC 2 cut(s) 155, 1081
BsmI GAATGC 3 cut(s) 935, 1188, 1200
BsnI GGCC 6 cut(s) 97, 628, 888, 968, 1085, 1120
Bso31I GGTCTC 1 cut(s) 786
Bsp119I TTCGAA 1 cut(s) 1192
Bsp1286I GDGCHC 4 cut(s) 457, 556, 583, 1108
Bsp143I GATC 3 cut(s) 103, 221, 773
BspANI GGCC 6 cut(s) 97, 628, 888, 968, 1085, 1120
BspCNI CTCAG 3 cut(s) 221, 231, 331
BspDI ATCGAT 1 cut(s) 756
BspLI GGNNCC 3 cut(s) 611, 887, 1097
BspMI ACCTGC 1 cut(s) 1009
BspPI GGATC 2 cut(s) 216, 768
BspT104I TTCGAA 1 cut(s) 1192
BspTNI GGTCTC 1 cut(s) 786
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 2 cut(s) 969, 993
BssECI CCNNGG 4 cut(s) 306, 629, 881, 1133
BssMI GATC 3 cut(s) 103, 221, 773
BssT1I CCWWGG 2 cut(s) 629, 1133
Bst2UI CCWGG 3 cut(s) 99, 214, 778
Bst4CI ACNGT 5 cut(s) 418, 799, 877, 1103, 1178
Bst6I CTCTTC 4 cut(s) 110, 531, 844, 1133
BstBI TTCGAA 1 cut(s) 1192
BstC8I GCNNGC 6 cut(s) 261, 480, 540, 579, 626, 890
BstDEI CTNAG 4 cut(s) 208, 218, 318, 458
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 45, 736
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 3 cut(s) 106, 224, 776
BstMAI GTCTC 4 cut(s) 230, 363, 786, 1014
BstMBI GATC 3 cut(s) 103, 221, 773
BstNI CCWGG 3 cut(s) 99, 214, 778
BstPAI GACNNNNGTC 1 cut(s) 563
BstSCI CCNGG 5 cut(s) 97, 171, 212, 776, 880
BstSFI CTRYAG 1 cut(s) 942
BstSLI GKGCMC 1 cut(s) 1108
BstV2I GAAGAC 1 cut(s) 825
BstX2I RGATCY 2 cut(s) 221, 773
BstYI RGATCY 2 cut(s) 221, 773
Bsu15I ATCGAT 1 cut(s) 756
BsuRI GGCC 6 cut(s) 97, 628, 888, 968, 1085, 1120
BsuTUI ATCGAT 1 cut(s) 756
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 2 cut(s) 584, 823
BtsCI GGATG 2 cut(s) 45, 736
BtsI GCAGTG 3 cut(s) 282, 845, 918
BtsIMutI CAGTG 6 cut(s) 282, 795, 845, 918, 1108, 1183
BveI ACCTGC 1 cut(s) 1009
Cac8I GCNNGC 6 cut(s) 261, 480, 540, 579, 626, 890
CfoI GCGC 1 cut(s) 448
Cfr13I GGNCC 2 cut(s) 886, 1095
ClaI ATCGAT 1 cut(s) 756
CseI GACGC 1 cut(s) 308
Csp6I GTAC 5 cut(s) 78, 878, 913, 954, 996
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 3 cut(s) 167, 530, 852
CviQI GTAC 5 cut(s) 78, 878, 913, 954, 996
DdeI CTNAG 4 cut(s) 208, 218, 318, 458
DpnI GATC 3 cut(s) 105, 223, 775
DpnII GATC 3 cut(s) 103, 221, 773
EaeI YGGCCR 1 cut(s) 966
Eam1104I CTCTTC 4 cut(s) 110, 531, 844, 1133
EarI CTCTTC 4 cut(s) 110, 531, 844, 1133
Ecl136II GAGCTC 1 cut(s) 455
Eco130I CCWWGG 2 cut(s) 629, 1133
Eco147I AGGCCT 2 cut(s) 628, 1120
Eco24I GRGCYC 1 cut(s) 457
Eco31I GGTCTC 1 cut(s) 786
Eco47I GGWCC 1 cut(s) 1095
Eco53kI GAGCTC 1 cut(s) 455
Eco57I CTGAAG 2 cut(s) 748, 881
EcoICRI GAGCTC 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 1095
EcoRII CCWGG 3 cut(s) 97, 212, 776
EcoT14I CCWWGG 2 cut(s) 629, 1133
EcoT22I ATGCAT 1 cut(s) 1200
EcoT38I GRGCYC 1 cut(s) 457
ErhI CCWWGG 2 cut(s) 629, 1133
FaeI CATG 3 cut(s) 170, 533, 855
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 155, 1081
FatI CATG 3 cut(s) 166, 529, 851
FokI GGATG 2 cut(s) 52, 743
FriOI GRGCYC 1 cut(s) 457
FspBI CTAG 4 cut(s) 12, 630, 1065, 1134
GlaI GCGC 1 cut(s) 447
HaeIII GGCC 6 cut(s) 97, 628, 888, 968, 1085, 1120
HapII CCGG 2 cut(s) 173, 881
HgaI GACGC 1 cut(s) 308
HhaI GCGC 1 cut(s) 448
Hin1II CATG 3 cut(s) 170, 533, 855
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HinfI GANTC 2 cut(s) 206, 409
HpaII CCGG 2 cut(s) 173, 881
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 3 cut(s) 939, 998, 1106
Hpy188I TCNGA 6 cut(s) 108, 221, 414, 425, 767, 808
Hpy188III TCNNGA 4 cut(s) 62, 505, 899, 1231
Hpy8I GTNNAC 3 cut(s) 939, 998, 1106
Hpy99I CGWCG 1 cut(s) 588
HpyAV CCTTC 6 cut(s) 19, 114, 238, 436, 723, 772
HpyCH4III ACNGT 5 cut(s) 418, 799, 877, 1103, 1178
HpyCH4IV ACGT 2 cut(s) 583, 822
HpyCH4V TGCA 8 cut(s) 152, 478, 538, 701, 933, 989, 1106, 1198
HpyF3I CTNAG 4 cut(s) 208, 218, 318, 458
HpySE526I ACGT 2 cut(s) 583, 822
Hsp92II CATG 3 cut(s) 170, 533, 855
HspAI GCGC 1 cut(s) 446
Kzo9I GATC 3 cut(s) 103, 221, 773
LweI GCATC 2 cut(s) 139, 1207
MaeI CTAG 4 cut(s) 12, 630, 1065, 1134
MaeII ACGT 2 cut(s) 583, 822
MalI GATC 3 cut(s) 105, 223, 775
MboI GATC 3 cut(s) 103, 221, 773
MboII GAAGA 9 cut(s) 27, 55, 127, 173, 518, 830, 831, 1150, 1235
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 2 cut(s) 221, 773
MhlI GDGCHC 4 cut(s) 457, 556, 583, 1108
MlsI TGGCCA 1 cut(s) 968
MluCI AATT 5 cut(s) 159, 264, 677, 858, 1234
MluNI TGGCCA 1 cut(s) 968
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 887
Mox20I TGGCCA 1 cut(s) 968
Mph1103I ATGCAT 1 cut(s) 1200
MroXI GAANNNNTTC 1 cut(s) 608
MscI TGGCCA 1 cut(s) 968
MseI TTAA 3 cut(s) 471, 720, 1080
MslI CAYNNNNRTG 2 cut(s) 850, 1193
Msp20I TGGCCA 1 cut(s) 968
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 2 cut(s) 173, 881
MspR9I CCNGG 5 cut(s) 99, 173, 214, 778, 882
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 3 cut(s) 935, 1188, 1200
MvaI CCWGG 3 cut(s) 99, 214, 778
NciI CCSGG 2 cut(s) 173, 882
NdeII GATC 3 cut(s) 103, 221, 773
NlaIII CATG 3 cut(s) 170, 533, 855
NlaIV GGNNCC 3 cut(s) 611, 887, 1097
NmeAIII GCCGAG 2 cut(s) 73, 259
NsiI ATGCAT 1 cut(s) 1200
NspV TTCGAA 1 cut(s) 1192
PaqCI CACCTGC 1 cut(s) 1009
PceI AGGCCT 2 cut(s) 628, 1120
PcsI WCGNNNNNNNCGW 1 cut(s) 1198
PctI GAATGC 3 cut(s) 935, 1188, 1200
PdmI GAANNNNTTC 1 cut(s) 608
PfeI GAWTC 1 cut(s) 409
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 1095
PshAI GACNNNNGTC 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 457
Psp5II RGGWCCY 1 cut(s) 1095
Psp6I CCWGG 3 cut(s) 97, 212, 776
PspGI CCWGG 3 cut(s) 97, 212, 776
PspN4I GGNNCC 3 cut(s) 611, 887, 1097
PspPI GGNCC 2 cut(s) 886, 1095
PspPPI RGGWCCY 1 cut(s) 1095
PsuI RGATCY 2 cut(s) 221, 773
RsaI GTAC 5 cut(s) 79, 879, 914, 955, 997
RsaNI GTAC 5 cut(s) 78, 878, 913, 954, 996
RseI CAYNNNNRTG 2 cut(s) 850, 1193
SacI GAGCTC 1 cut(s) 457
SaqAI TTAA 3 cut(s) 471, 720, 1080
Sau3AI GATC 3 cut(s) 103, 221, 773
Sau96I GGNCC 2 cut(s) 886, 1095
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 5 cut(s) 99, 173, 214, 778, 882
SduI GDGCHC 4 cut(s) 457, 556, 583, 1108
SfaNI GCATC 2 cut(s) 139, 1207
SfcI CTRYAG 1 cut(s) 942
SfuI TTCGAA 1 cut(s) 1192
SinI GGWCC 1 cut(s) 1095
SmiMI CAYNNNNRTG 2 cut(s) 850, 1193
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 5 cut(s) 159, 264, 677, 858, 1234
SseBI AGGCCT 2 cut(s) 628, 1120
SspI AATATT 1 cut(s) 1161
SspMI CTAG 4 cut(s) 12, 630, 1065, 1134
SstI GAGCTC 1 cut(s) 457
StuI AGGCCT 2 cut(s) 628, 1120
StyD4I CCNGG 5 cut(s) 97, 171, 212, 776, 880
StyI CCWWGG 2 cut(s) 629, 1133
TaaI ACNGT 5 cut(s) 418, 799, 877, 1103, 1178
TaiI ACGT 2 cut(s) 586, 825
TaqI TCGA 3 cut(s) 756, 1192, 1201
TaqII GACCGA 1 cut(s) 969
TasI AATT 5 cut(s) 159, 264, 677, 858, 1234
TatI WGTACW 2 cut(s) 77, 995
TauI GCSGC 6 cut(s) 290, 293, 326, 360, 401, 829
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 3 cut(s) 471, 720, 1080
Tru9I TTAA 3 cut(s) 471, 720, 1080
TscAI CASTG 6 cut(s) 289, 802, 852, 925, 1108, 1183
TspDTI ATGAA 6 cut(s) 56, 518, 678, 723, 747, 942
TspRI CASTG 6 cut(s) 289, 802, 852, 925, 1108, 1183
VneI GTGCAC 1 cut(s) 1104
VpaK11BI GGWCC 1 cut(s) 1095
XapI RAATTY 1 cut(s) 677
XmaJI CCTAGG 2 cut(s) 629, 1133
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 4 cut(s) 12, 630, 1065, 1134
ZrmI AGTACT 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.