MD02G1066900.v1.1

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0002379
Physical Location & Seq
Forward (+)
5474670 .. 5476598
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1066900.v1.1.491

Sequence Viewer

Length: 1296 bp
ATGGGCAGAGCAACTAGGTGGCTCAAAGGCTTGTTTGGAATAAAGACCAAAGACAAACACCAAAAACCAATTTCTGCTTCCGGCGACTTCAAAGCCAAGAAAGGCTCGAGCTTTGGGAGGGAGGATGCAGCCGCAACCCTGCTCTGCCACAGCCCCGCCACCATCCCGCCCAACATTTCGCCGTCCGAGGTGGCTTGGCTGAGATCCTACTACACAGAAACAGAGAAGGAGCAGAACAAGCAGGCAATTGCCGTGGCGGCTGCCACGTCTGCTGCTGCCGACTTTGCGGTTGCAGCGGCAAACGCGGCCGTGGCGGTTGTCCGCCTCACGAGTCATGGTAGAGGCACCATGTTCGGCGGCGCGAGAGAGAGATGGGCCGCTGTCAAAGTTCAAACTTGTTTCAGAGGCTATTTGGCTAGAAAAGCTCTAAGAGCTCTGAAAGGACTGGTGAAGTTGCAGGCATTAGTGAGAGGCTACTTAGTGAGGAAGCAGGCGACTGCAACTCTCCACAGTATGCAAGCTCTAATCCGAGCTCAAGCAACTGTTCGTTCTCATAAAAATCGGGGGCTGATCAGCATTGAATCCAGCAACAGATTTGAAATCCGTGCACGAAAATCCATGGAGAGATTTGGTGACAGCAGGAGTGAGCATACAGCTCCATCCCACAGCAAAAGGCTCTCTGCTTCTAATTCGGGTGACAATAATTTCAACGACATTGATGAAAGTCCCAAGATTGTTGAGGTTGATACAGGCAGCAGGCCGAAATCCAGGTCCCGGAGAACAAACACTGGCTCTGCATCCGTATTCTCCGACGACACGACATATCAGCGAGCATTGTCTTCTCCAATCCCGTATCCGGGTACTCCTGCCCGGCTGTCAATCCCCGACAGCCGGAACTACCAAGATTACGACTGGGCGCTTTCCGTGGAGGAATGCAGGATGATCTCCACGGCACAAAGTACGCCACGGTTCGTGAATTCCTGTGGCGGAGGGGCTACTAGCAATGCCGCCTGCACGCCTTCGAAGAGCGTTTGTAACGAAAATTATTTTAGAGGGTATTCGCCGAATTACATGGCGAGCACTCAGTCGTTCAAGGCGAAGTTGCGGTCTCATTCCGCTCCGAAACAGAGGCCGGAGCCGGGGGCAAAAAAGCGGCTTTCGCTGAACGAAATGTTGGCGTCGAGGAATAGTTTGAGTGGGGTTAGGATGCAGAGTTCCTGCTCGCAAGTGCAGGACGCCATTGTTTTCAAGAATGCTGTGATGGGGAAGCTTGAGAGGTCCTCTGGATTTTGGTAG

Protein Analysis

432

Amino Acids

47.03

Weight (kDa)

10.24

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 322 - 400 3.6e-15 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 344
AccBSI CCGCTC 1 cut(s) 1118
AccII CGCG 2 cut(s) 305, 362
AclWI GGATC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 306
AcsI RAATTY 1 cut(s) 976
AcyI GRCGYC 2 cut(s) 1178, 1236
AfaI GTAC 2 cut(s) 862, 961
AfiI CCNNNNNNNGG 5 cut(s) 774, 856, 857, 891, 1139
AgsI TTSAA 7 cut(s) 91, 392, 581, 599, 709, 1093, 1249
AjiI CACGTC 1 cut(s) 267
AjnI CCWGG 1 cut(s) 767
AjuI GAANNNNNNNTTGG 4 cut(s) 61, 93, 1157, 1189
AloI GAACNNNNNNTCC 2 cut(s) 1198, 1230
AluBI AGCT 7 cut(s) 111, 425, 434, 521, 533, 656, 1270
AluI AGCT 7 cut(s) 111, 425, 434, 521, 533, 656, 1270
Alw21I GWGCWC 4 cut(s) 436, 535, 610, 1082
Alw26I GTCTC 1 cut(s) 1113
Alw44I GTGCAC 1 cut(s) 606
AlwI GGATC 1 cut(s) 198
Ama87I CYCGRG 1 cut(s) 106
AoxI GGCC 4 cut(s) 306, 375, 758, 1130
ApaLI GTGCAC 1 cut(s) 606
ApeKI GCWGC 6 cut(s) 128, 260, 272, 275, 293, 753
ApoI RAATTY 1 cut(s) 976
ArsI GACNNNNNNTTYG 4 cut(s) 755, 787, 1091, 1123
AspLEI GCGC 2 cut(s) 362, 919
AspS9I GGNCC 3 cut(s) 375, 771, 1278
AsuC2I CCSGG 4 cut(s) 775, 858, 871, 1140
AsuHPI GGTGA 3 cut(s) 460, 644, 707
AsuII TTCGAA 1 cut(s) 1022
AvaI CYCGRG 1 cut(s) 106
AvaII GGWCC 2 cut(s) 771, 1278
BaeGI GKGCMC 1 cut(s) 610
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 2 cut(s) 436, 535
BauI CACGAG 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 4 cut(s) 436, 535, 610, 1082
BbvI GCAGC 6 cut(s) 140, 247, 259, 262, 305, 765
BccI CCATC 4 cut(s) 170, 366, 667, 1255
BceAI ACGGC 4 cut(s) 166, 236, 293, 966
BcgI CGANNNNNNTGC 2 cut(s) 334, 368
BciT130I CCWGG 1 cut(s) 769
BciVI GTATCC 1 cut(s) 864
BclI TGATCA 1 cut(s) 570
BcnI CCSGG 4 cut(s) 775, 858, 871, 1140
BcoDI GTCTC 1 cut(s) 1113
BfaI CTAG 3 cut(s) 15, 417, 999
BfoI RGCGCY 1 cut(s) 920
BfuI GTATCC 1 cut(s) 864
BglI GCCNNNNNGGC 1 cut(s) 257
Bme1390I CCNGG 5 cut(s) 769, 775, 858, 871, 1140
Bme18I GGWCC 2 cut(s) 771, 1278
BmeT110I CYCGRG 1 cut(s) 106
BmgBI CACGTC 1 cut(s) 267
BmgT120I GGNCC 3 cut(s) 375, 771, 1278
BmiI GGNNCC 3 cut(s) 346, 773, 1137
BmrFI CCNGG 5 cut(s) 769, 775, 858, 871, 1140
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 3 cut(s) 115, 806, 1197
BmuI ACTGGG 1 cut(s) 922
BpiI GAAGAC 1 cut(s) 831
BplI GAGNNNNNCTC 1 cut(s) 1265
Bpu14I TTCGAA 1 cut(s) 1022
BpuEI CTTGAG 2 cut(s) 519, 1292
BpuMI CCSGG 4 cut(s) 775, 858, 871, 1140
BsaHI GRCGYC 2 cut(s) 1178, 1236
BsaI GGTCTC 1 cut(s) 1113
BsaJI CCNNGG 8 cut(s) 186, 252, 309, 618, 924, 948, 965, 1139
Bsc4I CCNNNNNNNGG 5 cut(s) 774, 856, 857, 891, 1139
Bse1I ACTGG 3 cut(s) 450, 793, 917
Bse3DI GCAATG 1 cut(s) 1009
BseBI CCWGG 1 cut(s) 769
BseDI CCNNGG 8 cut(s) 186, 252, 309, 618, 924, 948, 965, 1139
BseGI GGATG 6 cut(s) 130, 162, 659, 797, 945, 1212
BseLI CCNNNNNNNGG 5 cut(s) 774, 856, 857, 891, 1139
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 2 cut(s) 191, 1097
BseNI ACTGG 3 cut(s) 450, 793, 917
BseSI GKGCMC 1 cut(s) 610
BseX3I CGGCCG 1 cut(s) 306
BseXI GCAGC 6 cut(s) 140, 247, 259, 262, 305, 765
BsgI GTGCAG 2 cut(s) 997, 1250
Bsh1236I CGCG 2 cut(s) 305, 362
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 4 cut(s) 308, 377, 760, 1132
BshNI GGYRCC 1 cut(s) 344
BsiEI CGRYCG 1 cut(s) 309
BsiHKAI GWGCWC 4 cut(s) 436, 535, 610, 1082
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 7 cut(s) 81, 775, 857, 871, 892, 1133, 1139
BslFI GGGAC 2 cut(s) 711, 757
BslI CCNNNNNNNGG 5 cut(s) 774, 856, 857, 891, 1139
BsmAI GTCTC 1 cut(s) 1113
BsmFI GGGAC 2 cut(s) 711, 757
BsmI GAATGC 2 cut(s) 938, 1258
BsnI GGCC 4 cut(s) 308, 377, 760, 1132
Bso31I GGTCTC 1 cut(s) 1113
BsoBI CYCGRG 1 cut(s) 106
Bsp119I TTCGAA 1 cut(s) 1022
Bsp1286I GDGCHC 4 cut(s) 436, 535, 610, 1082
Bsp143I GATC 3 cut(s) 203, 570, 942
Bsp19I CCATGG 1 cut(s) 618
BspANI GGCC 4 cut(s) 308, 377, 760, 1132
BspCNI CTCAG 2 cut(s) 192, 1096
BspFNI CGCG 2 cut(s) 305, 362
BspLI GGNNCC 3 cut(s) 346, 773, 1137
BspPI GGATC 1 cut(s) 198
BspQI GCTCTTC 1 cut(s) 1019
BspT104I TTCGAA 1 cut(s) 1022
BspT107I GGYRCC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 1113
BsrBI CCGCTC 1 cut(s) 1118
BsrDI GCAATG 1 cut(s) 1009
BsrI ACTGG 3 cut(s) 450, 793, 917
BssECI CCNNGG 8 cut(s) 186, 252, 309, 618, 924, 948, 965, 1139
BssMI GATC 3 cut(s) 203, 570, 942
BssNI GRCGYC 2 cut(s) 1178, 1236
BssSI CACGAG 1 cut(s) 328
BssT1I CCWWGG 1 cut(s) 618
Bst2BI CACGAG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 769
Bst4CI ACNGT 3 cut(s) 512, 544, 969
Bst6I CTCTTC 1 cut(s) 1019
BstACI GRCGYC 2 cut(s) 1178, 1236
BstBI TTCGAA 1 cut(s) 1022
BstDEI CTNAG 4 cut(s) 200, 428, 478, 1083
BstDSI CCRYGG 6 cut(s) 252, 309, 618, 924, 948, 965
BstF5I GGATG 6 cut(s) 130, 162, 659, 797, 945, 1212
BstFNI CGCG 2 cut(s) 305, 362
BstH2I RGCGCY 1 cut(s) 920
BstHHI GCGC 2 cut(s) 362, 919
BstKTI GATC 3 cut(s) 206, 573, 945
BstMAI GTCTC 1 cut(s) 1113
BstMBI GATC 3 cut(s) 203, 570, 942
BstMCI CGRYCG 1 cut(s) 309
BstNI CCWGG 1 cut(s) 769
BstSCI CCNGG 5 cut(s) 767, 773, 856, 869, 1138
BstSLI GKGCMC 1 cut(s) 610
BstUI CGCG 2 cut(s) 305, 362
BstV1I GCAGC 6 cut(s) 140, 247, 259, 262, 305, 765
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 1 cut(s) 203
BstYI RGATCY 1 cut(s) 203
BstZI CGGCCG 1 cut(s) 306
BsuI GTATCC 1 cut(s) 864
BsuRI GGCC 4 cut(s) 308, 377, 760, 1132
BtgI CCRYGG 6 cut(s) 252, 309, 618, 924, 948, 965
BtrI CACGTC 1 cut(s) 267
BtsCI GGATG 6 cut(s) 130, 162, 659, 797, 945, 1212
BtsIMutI CAGTG 1 cut(s) 786
CfoI GCGC 2 cut(s) 362, 919
Cfr13I GGNCC 3 cut(s) 375, 771, 1278
CseI GACGC 2 cut(s) 1167, 1244
Csp6I GTAC 2 cut(s) 861, 960
CspCI CAANNNNNGTGG 2 cut(s) 234, 269
CviAII CATG 4 cut(s) 335, 349, 619, 1072
CviQI GTAC 2 cut(s) 861, 960
DdeI CTNAG 4 cut(s) 200, 428, 478, 1083
DpnI GATC 3 cut(s) 205, 572, 944
DpnII GATC 3 cut(s) 203, 570, 942
EaeI YGGCCR 1 cut(s) 306
EagI CGGCCG 1 cut(s) 306
Eam1104I CTCTTC 1 cut(s) 1019
EarI CTCTTC 1 cut(s) 1019
EciI GGCGGA 2 cut(s) 311, 1002
Ecl136II GAGCTC 2 cut(s) 434, 533
EclXI CGGCCG 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 618
Eco24I GRGCYC 2 cut(s) 436, 535
Eco31I GGTCTC 1 cut(s) 1113
Eco47I GGWCC 2 cut(s) 771, 1278
Eco52I CGGCCG 1 cut(s) 306
Eco53kI GAGCTC 2 cut(s) 434, 533
Eco88I CYCGRG 1 cut(s) 106
EcoICRI GAGCTC 2 cut(s) 434, 533
EcoO109I RGGNCCY 2 cut(s) 771, 1278
EcoRI GAATTC 1 cut(s) 976
EcoRII CCWGG 1 cut(s) 767
EcoT14I CCWWGG 1 cut(s) 618
EcoT38I GRGCYC 2 cut(s) 436, 535
ErhI CCWWGG 1 cut(s) 618
FaeI CATG 4 cut(s) 338, 352, 622, 1075
FaiI YATR 8 cut(s) 336, 350, 515, 555, 620, 651, 823, 1073
FaqI GGGAC 2 cut(s) 711, 757
FatI CATG 4 cut(s) 334, 348, 618, 1071
FauI CCCGC 2 cut(s) 163, 174
FbaI TGATCA 1 cut(s) 570
FokI GGATG 6 cut(s) 137, 149, 646, 784, 952, 1219
FriOI GRGCYC 2 cut(s) 436, 535
FspBI CTAG 3 cut(s) 15, 417, 999
GlaI GCGC 2 cut(s) 361, 918
HaeII RGCGCY 1 cut(s) 920
HaeIII GGCC 4 cut(s) 308, 377, 760, 1132
HapII CCGG 7 cut(s) 81, 775, 857, 871, 892, 1133, 1139
HgaI GACGC 2 cut(s) 1167, 1244
HhaI GCGC 2 cut(s) 362, 919
Hin1I GRCGYC 2 cut(s) 1178, 1236
Hin1II CATG 4 cut(s) 338, 352, 622, 1075
Hin6I GCGC 2 cut(s) 360, 917
HinP1I GCGC 2 cut(s) 360, 917
HindIII AAGCTT 1 cut(s) 1268
HinfI GANTC 2 cut(s) 331, 581
HpaII CCGG 7 cut(s) 81, 775, 857, 871, 892, 1133, 1139
HphI GGTGA 3 cut(s) 460, 644, 707
Hpy166II GTNNAC 1 cut(s) 608
Hpy188I TCNGA 6 cut(s) 187, 404, 438, 530, 811, 1122
Hpy188III TCNNGA 4 cut(s) 328, 973, 1249, 1284
Hpy8I GTNNAC 1 cut(s) 608
Hpy99I CGWCG 2 cut(s) 815, 1183
HpyAV CCTTC 2 cut(s) 220, 1029
HpyCH4III ACNGT 3 cut(s) 512, 544, 969
HpyCH4IV ACGT 1 cut(s) 266
HpyF3I CTNAG 4 cut(s) 200, 428, 478, 1083
HpySE526I ACGT 1 cut(s) 266
Hsp92I GRCGYC 2 cut(s) 1178, 1236
Hsp92II CATG 4 cut(s) 338, 352, 622, 1075
HspAI GCGC 2 cut(s) 360, 917
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 3 cut(s) 203, 570, 942
LguI GCTCTTC 1 cut(s) 1019
LmnI GCTCC 4 cut(s) 229, 661, 1123, 1135
Lsp1109I GCAGC 6 cut(s) 140, 247, 259, 262, 305, 765
LweI GCATC 3 cut(s) 115, 806, 1197
MaeI CTAG 3 cut(s) 15, 417, 999
MaeII ACGT 1 cut(s) 266
MaeIII GTNAC 3 cut(s) 632, 695, 1034
MalI GATC 3 cut(s) 205, 572, 944
MbiI CCGCTC 1 cut(s) 1118
MboI GATC 3 cut(s) 203, 570, 942
MboII GAAGA 2 cut(s) 831, 1036
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 203
MhlI GDGCHC 4 cut(s) 436, 535, 610, 1082
MluCI AATT 7 cut(s) 69, 246, 688, 703, 976, 1042, 1066
MlyI GAGTC 1 cut(s) 340
MmeI TCCRAC 1 cut(s) 834
MspA1I CMGCKG 2 cut(s) 296, 380
MspI CCGG 7 cut(s) 81, 775, 857, 871, 892, 1133, 1139
MspR9I CCNGG 5 cut(s) 769, 775, 858, 871, 1140
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 2 cut(s) 938, 1258
MvaI CCWGG 1 cut(s) 769
MvnI CGCG 2 cut(s) 305, 362
NciI CCSGG 4 cut(s) 775, 858, 871, 1140
NcoI CCATGG 1 cut(s) 618
NdeII GATC 3 cut(s) 203, 570, 942
NlaIII CATG 4 cut(s) 338, 352, 622, 1075
NlaIV GGNNCC 3 cut(s) 346, 773, 1137
NmuCI GTSAC 2 cut(s) 632, 695
NspV TTCGAA 1 cut(s) 1022
PaeR7I CTCGAG 1 cut(s) 106
PciSI GCTCTTC 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 1 cut(s) 1094
PctI GAATGC 2 cut(s) 938, 1258
PfeI GAWTC 1 cut(s) 581
PfoI TCCNGGA 1 cut(s) 773
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
PpuMI RGGWCCY 2 cut(s) 771, 1278
Psp124BI GAGCTC 2 cut(s) 436, 535
Psp5II RGGWCCY 2 cut(s) 771, 1278
Psp6I CCWGG 1 cut(s) 767
PspGI CCWGG 1 cut(s) 767
PspN4I GGNNCC 3 cut(s) 346, 773, 1137
PspPI GGNCC 3 cut(s) 375, 771, 1278
PspPPI RGGWCCY 2 cut(s) 771, 1278
PspXI VCTCGAGB 1 cut(s) 106
PsuI RGATCY 1 cut(s) 203
RsaI GTAC 2 cut(s) 862, 961
RsaNI GTAC 2 cut(s) 861, 960
SacI GAGCTC 2 cut(s) 436, 535
SapI GCTCTTC 1 cut(s) 1019
Sau3AI GATC 3 cut(s) 203, 570, 942
Sau96I GGNCC 3 cut(s) 375, 771, 1278
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 5 cut(s) 769, 775, 858, 871, 1140
SduI GDGCHC 4 cut(s) 436, 535, 610, 1082
SfaNI GCATC 3 cut(s) 115, 806, 1197
Sfr274I CTCGAG 1 cut(s) 106
SfuI TTCGAA 1 cut(s) 1022
SinI GGWCC 2 cut(s) 771, 1278
SlaI CTCGAG 1 cut(s) 106
SmlI CTYRAG 3 cut(s) 106, 534, 1271
SmoI CTYRAG 3 cut(s) 106, 534, 1271
Sse9I AATT 7 cut(s) 69, 246, 688, 703, 976, 1042, 1066
SspMI CTAG 3 cut(s) 15, 417, 999
SstI GAGCTC 2 cut(s) 436, 535
StyD4I CCNGG 5 cut(s) 767, 773, 856, 869, 1138
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 3 cut(s) 512, 544, 969
TaiI ACGT 1 cut(s) 269
TaqI TCGA 3 cut(s) 107, 1022, 1181
TasI AATT 7 cut(s) 69, 246, 688, 703, 976, 1042, 1066
TauI GCSGC 8 cut(s) 134, 260, 299, 308, 360, 380, 1010, 1156
TfiI GAWTC 1 cut(s) 581
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 2 cut(s) 632, 695
TseI GCWGC 6 cut(s) 128, 260, 272, 275, 293, 753
Tsp45I GTSAC 2 cut(s) 632, 695
TspDTI ATGAA 1 cut(s) 735
TspGWI ACGGA 3 cut(s) 593, 790, 913
TspRI CASTG 1 cut(s) 793
VneI GTGCAC 1 cut(s) 606
VpaK11BI GGWCC 2 cut(s) 771, 1278
XapI RAATTY 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 106
XspI CTAG 3 cut(s) 15, 417, 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.