Rh2CG402700

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002379
Physical Location & Seq
Forward (+)
54782928 .. 54784776
1849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG402700.1

Sequence Viewer

Length: 1230 bp
ATGGGGAGAGCTAGAAGATGGTTGAAGGCTCTGTTGGGGATGAAGAAAGAGAAAGACCCTCTTGAGCTTCCAAATAGTACTACCAATACTACTCGGCCTGGTGATCGGAAAGAGAAGAGAAGGTGGAGTTTCGGAAAGTCATTGAAAGATGCAAGTCCAATTAGTCATGTCCCGGCTAATCTTCCCCCGAACGTTTCCCCTGCTGACTCAGCCTGGCTCAGATCCTATCTTGCCGAGACAGAGAAGGAACAGAACAAGCACGCAATTGCGGTGGCTGCTGCCACTGCAGCCGCTGCTGACGCTGCCGTGGCTGCTGCTCATGCGGCGGTGGCAGTTGTGAGGCTGACGAGCAAGGGCGGCTGTGGAGGAGACTTGTTTGGTAAGAGAGAGAGATGGGCGGCAATGAGGATTCAGACTGTTTTCAGAGGCTATTTGGCCAGGAAGGCGCACCGAGCTCTTAGAGGACTTGTTAAGTTGCAGGCTCTTGTTAGAGGCTTTCTTGTCAGGAAACGAGCTGCTGCTACTCTTCATGGTATGCAAGCTCTTATTAGAGCACAGACAAGTGTCCGCTACCAGCGAGCACGTCGTTCTTTCAATAAAGAGAACAGGTTCCTACCCGAAAGCAGGCCTAGGAAGTCCATTGAGAGATTTGATGAAGCAAGAAGTGAATTTTATAGCAAGAGGCTTTCTGCAACTTACGAACCTTCTCTTAATGGCTTGGATGAAAACCCAAAGATTGTTGAAATCGATACCTTCAGAACCAGATCCAGGTCTCGCCGATACAACACTGTCTTATCTGAATATGGCGAAGACGTGCCTTACACCTCTTCGCCACTACCATGTCCAATTCCAACTCGCATTACTGTACCTGGGGGCCAGCAGCTTCAGGATTATGAATGGTACTTCACTGCTGATGAATGCAAGTTCACTACAGCCCATAGTACCCCCCGTTTGGCCAGTTCTTTTCGGTCTAATGCACCCAGTACACCTGCCAAGAGTGTTTGTGGAGACAGCTTCTTTCAGCCTTACTCAAACTGCCCAAACTATATGTCTAGCACCAAGTCATTTAAGGCCAAATCAAGGTCCCACAGTGCACCGAAGCAAAGGCCTGAACCCCTGCCTAGGAAGAGGCTTTCCCTTGATGAAATAATGGCAGCAAGAAACAGTGTGAGTGGCATTCGAATGCATCGACCCTACAACCAAGTCCAAGAAGAAGTCTTGAATTATTGA

Protein Analysis

409

Amino Acids

46.01

Weight (kDa)

10.33

Isoelectric Point (pI)

60.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 313 - 391 2.5e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 997
Acc36I ACCTGC 1 cut(s) 997
AciI CCGC 7 cut(s) 269, 291, 323, 326, 357, 398, 568
AclI AACGTT 1 cut(s) 192
AclWI GGATC 2 cut(s) 216, 759
AcoI YGGCCR 2 cut(s) 435, 954
AcsI RAATTY 1 cut(s) 668
AcuI CTGAAG 2 cut(s) 739, 869
AfaI GTAC 5 cut(s) 79, 867, 902, 943, 985
AfiI CCNNNNNNNGG 5 cut(s) 624, 768, 952, 1080, 1122
AgsI TTSAA 5 cut(s) 25, 145, 595, 743, 1222
AjiI CACGTC 2 cut(s) 584, 814
AjnI CCWGG 5 cut(s) 97, 212, 437, 767, 868
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 7 cut(s) 11, 67, 455, 515, 542, 883, 1014
AluI AGCT 7 cut(s) 11, 67, 455, 515, 542, 883, 1014
Alw21I GWGCWC 4 cut(s) 457, 556, 583, 1096
Alw26I GTCTC 4 cut(s) 230, 363, 777, 1002
Alw44I GTGCAC 1 cut(s) 1092
AlwI GGATC 2 cut(s) 216, 759
AlwNI CAGNNNCTG 1 cut(s) 293
AoxI GGCC 7 cut(s) 95, 435, 626, 874, 954, 1071, 1106
ApaLI GTGCAC 1 cut(s) 1092
ApoI RAATTY 1 cut(s) 668
ArsI GACNNNNNNTTYG 2 cut(s) 1034, 1066
Asp700I GAANNNNTTC 1 cut(s) 608
AspA2I CCTAGG 2 cut(s) 629, 1121
AspLEI GCGC 1 cut(s) 448
AspS9I GGNCC 2 cut(s) 874, 1083
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 113
AsuII TTCGAA 1 cut(s) 1180
AvaII GGWCC 1 cut(s) 1083
AvrII CCTAGG 2 cut(s) 629, 1121
BaeGI GKGCMC 1 cut(s) 1096
BalI TGGCCA 2 cut(s) 437, 956
BanII GRGCYC 1 cut(s) 457
BbsI GAAGAC 1 cut(s) 816
Bbv12I GWGCWC 4 cut(s) 457, 556, 583, 1096
BccI CCATC 2 cut(s) 12, 387
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 5 cut(s) 99, 214, 439, 769, 870
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 4 cut(s) 230, 363, 777, 1002
BfaI CTAG 4 cut(s) 12, 630, 1053, 1122
BfmI CTRYAG 2 cut(s) 285, 930
BfuAI ACCTGC 1 cut(s) 997
BglI GCCNNNNNGGC 1 cut(s) 443
BlnI CCTAGG 2 cut(s) 629, 1121
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 6 cut(s) 99, 173, 214, 439, 769, 870
Bme18I GGWCC 1 cut(s) 1083
BmgBI CACGTC 2 cut(s) 584, 814
BmgT120I GGNCC 2 cut(s) 874, 1083
BmiI GGNNCC 3 cut(s) 611, 875, 1085
BmrFI CCNGG 6 cut(s) 99, 173, 214, 439, 769, 870
BmrI ACTGGG 1 cut(s) 975
BmsI GCATC 2 cut(s) 139, 1195
BmuI ACTGGG 1 cut(s) 975
BoxI GACNNNNGTC 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 816
Bpu14I TTCGAA 1 cut(s) 1180
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 173
Bsa29I ATCGAT 1 cut(s) 747
BsaI GGTCTC 1 cut(s) 777
BsaJI CCNNGG 4 cut(s) 306, 629, 869, 1121
Bsc4I CCNNNNNNNGG 5 cut(s) 624, 768, 952, 1080, 1122
Bse1I ACTGG 2 cut(s) 957, 981
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 5 cut(s) 99, 214, 439, 769, 870
BseCI ATCGAT 1 cut(s) 747
BseDI CCNNGG 4 cut(s) 306, 629, 869, 1121
BseGI GGATG 2 cut(s) 45, 727
BseLI CCNNNNNNNGG 5 cut(s) 624, 768, 952, 1080, 1122
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 2 cut(s) 222, 232
BseNI ACTGG 2 cut(s) 957, 981
BseRI GAGGAG 1 cut(s) 381
BseSI GKGCMC 1 cut(s) 1096
BshFI GGCC 7 cut(s) 97, 437, 628, 876, 956, 1073, 1108
BshVI ATCGAT 1 cut(s) 747
BsiHKAI GWGCWC 4 cut(s) 457, 556, 583, 1096
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 2 cut(s) 155, 1069
BslI CCNNNNNNNGG 5 cut(s) 624, 768, 952, 1080, 1122
BsmAI GTCTC 4 cut(s) 230, 363, 777, 1002
BsmFI GGGAC 2 cut(s) 155, 1069
BsmI GAATGC 3 cut(s) 923, 1176, 1188
BsnI GGCC 7 cut(s) 97, 437, 628, 876, 956, 1073, 1108
Bso31I GGTCTC 1 cut(s) 777
Bsp119I TTCGAA 1 cut(s) 1180
Bsp1286I GDGCHC 4 cut(s) 457, 556, 583, 1096
Bsp143I GATC 3 cut(s) 103, 221, 764
BspACI CCGC 7 cut(s) 269, 291, 323, 326, 357, 398, 568
BspANI GGCC 7 cut(s) 97, 437, 628, 876, 956, 1073, 1108
BspCNI CTCAG 2 cut(s) 221, 231
BspDI ATCGAT 1 cut(s) 747
BspLI GGNNCC 3 cut(s) 611, 875, 1085
BspMAI CTGCAG 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 997
BspPI GGATC 2 cut(s) 216, 759
BspT104I TTCGAA 1 cut(s) 1180
BspTNI GGTCTC 1 cut(s) 777
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 2 cut(s) 957, 981
BssECI CCNNGG 4 cut(s) 306, 629, 869, 1121
BssMI GATC 3 cut(s) 103, 221, 764
BssT1I CCWWGG 2 cut(s) 629, 1121
Bst2UI CCWGG 5 cut(s) 99, 214, 439, 769, 870
Bst4CI ACNGT 5 cut(s) 418, 790, 865, 1091, 1166
Bst6I CTCTTC 4 cut(s) 110, 531, 832, 1121
BstAPI GCANNNNNTGC 1 cut(s) 293
BstBI TTCGAA 1 cut(s) 1180
BstC8I GCNNGC 6 cut(s) 261, 480, 540, 579, 626, 878
BstDEI CTNAG 3 cut(s) 208, 218, 458
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 45, 727
BstHHI GCGC 1 cut(s) 448
BstKTI GATC 3 cut(s) 106, 224, 767
BstMAI GTCTC 4 cut(s) 230, 363, 777, 1002
BstMBI GATC 3 cut(s) 103, 221, 764
BstNI CCWGG 5 cut(s) 99, 214, 439, 769, 870
BstPAI GACNNNNGTC 1 cut(s) 563
BstSCI CCNGG 6 cut(s) 97, 171, 212, 437, 767, 868
BstSFI CTRYAG 2 cut(s) 285, 930
BstSLI GKGCMC 1 cut(s) 1096
BstV2I GAAGAC 1 cut(s) 816
BstX2I RGATCY 2 cut(s) 221, 764
BstYI RGATCY 2 cut(s) 221, 764
Bsu15I ATCGAT 1 cut(s) 747
BsuRI GGCC 7 cut(s) 97, 437, 628, 876, 956, 1073, 1108
BsuTUI ATCGAT 1 cut(s) 747
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 2 cut(s) 584, 814
BtsCI GGATG 2 cut(s) 45, 727
BtsI GCAGTG 2 cut(s) 282, 906
BtsIMutI CAGTG 5 cut(s) 282, 786, 906, 1096, 1171
BveI ACCTGC 1 cut(s) 997
Cac8I GCNNGC 6 cut(s) 261, 480, 540, 579, 626, 878
CaiI CAGNNNCTG 1 cut(s) 293
CfoI GCGC 1 cut(s) 448
Cfr13I GGNCC 2 cut(s) 874, 1083
ClaI ATCGAT 1 cut(s) 747
CseI GACGC 1 cut(s) 308
Csp6I GTAC 5 cut(s) 78, 866, 901, 942, 984
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 4 cut(s) 167, 320, 530, 840
CviQI GTAC 5 cut(s) 78, 866, 901, 942, 984
DdeI CTNAG 3 cut(s) 208, 218, 458
DpnI GATC 3 cut(s) 105, 223, 766
DpnII GATC 3 cut(s) 103, 221, 764
EaeI YGGCCR 2 cut(s) 435, 954
Eam1104I CTCTTC 4 cut(s) 110, 531, 832, 1121
EarI CTCTTC 4 cut(s) 110, 531, 832, 1121
Ecl136II GAGCTC 1 cut(s) 455
Eco130I CCWWGG 2 cut(s) 629, 1121
Eco147I AGGCCT 2 cut(s) 628, 1108
Eco24I GRGCYC 1 cut(s) 457
Eco31I GGTCTC 1 cut(s) 777
Eco47I GGWCC 1 cut(s) 1083
Eco53kI GAGCTC 1 cut(s) 455
Eco57I CTGAAG 2 cut(s) 739, 869
EcoICRI GAGCTC 1 cut(s) 455
EcoO109I RGGNCCY 1 cut(s) 1083
EcoRII CCWGG 5 cut(s) 97, 212, 437, 767, 868
EcoT14I CCWWGG 2 cut(s) 629, 1121
EcoT22I ATGCAT 1 cut(s) 1188
EcoT38I GRGCYC 1 cut(s) 457
ErhI CCWWGG 2 cut(s) 629, 1121
FaeI CATG 4 cut(s) 170, 323, 533, 843
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 155, 1069
FatI CATG 4 cut(s) 166, 319, 529, 839
FokI GGATG 2 cut(s) 52, 734
FriOI GRGCYC 1 cut(s) 457
FspBI CTAG 4 cut(s) 12, 630, 1053, 1122
GlaI GCGC 1 cut(s) 447
HaeIII GGCC 7 cut(s) 97, 437, 628, 876, 956, 1073, 1108
HapII CCGG 1 cut(s) 173
HgaI GACGC 1 cut(s) 308
HhaI GCGC 1 cut(s) 448
Hin1II CATG 4 cut(s) 170, 323, 533, 843
Hin6I GCGC 1 cut(s) 446
HinP1I GCGC 1 cut(s) 446
HinfI GANTC 2 cut(s) 206, 409
HpaII CCGG 1 cut(s) 173
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 3 cut(s) 927, 986, 1094
Hpy188I TCNGA 7 cut(s) 108, 134, 221, 414, 425, 758, 799
Hpy188III TCNNGA 4 cut(s) 62, 505, 887, 1219
Hpy8I GTNNAC 3 cut(s) 927, 986, 1094
Hpy99I CGWCG 1 cut(s) 588
HpyAV CCTTC 6 cut(s) 19, 114, 238, 436, 714, 763
HpyCH4III ACNGT 5 cut(s) 418, 790, 865, 1091, 1166
HpyCH4IV ACGT 3 cut(s) 192, 583, 813
HpyCH4V TGCA 9 cut(s) 152, 287, 478, 538, 692, 921, 977, 1094, 1186
HpyF3I CTNAG 3 cut(s) 208, 218, 458
HpySE526I ACGT 3 cut(s) 192, 583, 813
Hsp92II CATG 4 cut(s) 170, 323, 533, 843
HspAI GCGC 1 cut(s) 446
Kzo9I GATC 3 cut(s) 103, 221, 764
LweI GCATC 2 cut(s) 139, 1195
MaeI CTAG 4 cut(s) 12, 630, 1053, 1122
MaeII ACGT 3 cut(s) 192, 583, 813
MalI GATC 3 cut(s) 105, 223, 766
MboI GATC 3 cut(s) 103, 221, 764
MboII GAAGA 9 cut(s) 27, 55, 127, 173, 518, 819, 821, 1138, 1223
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 2 cut(s) 221, 764
MhlI GDGCHC 4 cut(s) 457, 556, 583, 1096
MlsI TGGCCA 2 cut(s) 437, 956
MluCI AATT 5 cut(s) 159, 264, 668, 846, 1222
MluNI TGGCCA 2 cut(s) 437, 956
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 875
Mox20I TGGCCA 2 cut(s) 437, 956
Mph1103I ATGCAT 1 cut(s) 1188
MroXI GAANNNNTTC 1 cut(s) 608
MscI TGGCCA 2 cut(s) 437, 956
MseI TTAA 3 cut(s) 471, 711, 1068
MslI CAYNNNNRTG 2 cut(s) 838, 1181
Msp20I TGGCCA 2 cut(s) 437, 956
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 6 cut(s) 99, 173, 214, 439, 769, 870
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 3 cut(s) 923, 1176, 1188
MvaI CCWGG 5 cut(s) 99, 214, 439, 769, 870
NciI CCSGG 1 cut(s) 173
NdeII GATC 3 cut(s) 103, 221, 764
NlaIII CATG 4 cut(s) 170, 323, 533, 843
NlaIV GGNNCC 3 cut(s) 611, 875, 1085
NmeAIII GCCGAG 2 cut(s) 73, 259
NsiI ATGCAT 1 cut(s) 1188
NspV TTCGAA 1 cut(s) 1180
PaqCI CACCTGC 1 cut(s) 997
PceI AGGCCT 2 cut(s) 628, 1108
PcsI WCGNNNNNNNCGW 1 cut(s) 1186
PctI GAATGC 3 cut(s) 923, 1176, 1188
PdmI GAANNNNTTC 1 cut(s) 608
PfeI GAWTC 1 cut(s) 409
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 1083
PshAI GACNNNNGTC 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 457
Psp1406I AACGTT 1 cut(s) 192
Psp5II RGGWCCY 1 cut(s) 1083
Psp6I CCWGG 5 cut(s) 97, 212, 437, 767, 868
PspGI CCWGG 5 cut(s) 97, 212, 437, 767, 868
PspN4I GGNNCC 3 cut(s) 611, 875, 1085
PspPI GGNCC 2 cut(s) 874, 1083
PspPPI RGGWCCY 1 cut(s) 1083
PstI CTGCAG 1 cut(s) 289
PstNI CAGNNNCTG 1 cut(s) 293
PsuI RGATCY 2 cut(s) 221, 764
RsaI GTAC 5 cut(s) 79, 867, 902, 943, 985
RsaNI GTAC 5 cut(s) 78, 866, 901, 942, 984
RseI CAYNNNNRTG 2 cut(s) 838, 1181
SacI GAGCTC 1 cut(s) 457
SaqAI TTAA 3 cut(s) 471, 711, 1068
Sau3AI GATC 3 cut(s) 103, 221, 764
Sau96I GGNCC 2 cut(s) 874, 1083
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 6 cut(s) 99, 173, 214, 439, 769, 870
SduI GDGCHC 4 cut(s) 457, 556, 583, 1096
SfaNI GCATC 2 cut(s) 139, 1195
SfcI CTRYAG 2 cut(s) 285, 930
SfuI TTCGAA 1 cut(s) 1180
SinI GGWCC 1 cut(s) 1083
SmiMI CAYNNNNRTG 2 cut(s) 838, 1181
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 5 cut(s) 159, 264, 668, 846, 1222
SseBI AGGCCT 2 cut(s) 628, 1108
SsiI CCGC 7 cut(s) 269, 291, 323, 326, 357, 398, 568
SspMI CTAG 4 cut(s) 12, 630, 1053, 1122
SstI GAGCTC 1 cut(s) 457
StuI AGGCCT 2 cut(s) 628, 1108
StyD4I CCNGG 6 cut(s) 97, 171, 212, 437, 767, 868
StyI CCWWGG 2 cut(s) 629, 1121
TaaI ACNGT 5 cut(s) 418, 790, 865, 1091, 1166
TaiI ACGT 3 cut(s) 195, 586, 816
TaqI TCGA 3 cut(s) 747, 1180, 1189
TaqII GACCGA 1 cut(s) 957
TasI AATT 5 cut(s) 159, 264, 668, 846, 1222
TatI WGTACW 2 cut(s) 77, 983
TauI GCSGC 4 cut(s) 293, 326, 360, 401
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 3 cut(s) 471, 711, 1068
Tru9I TTAA 3 cut(s) 471, 711, 1068
TscAI CASTG 5 cut(s) 289, 793, 913, 1096, 1171
TspDTI ATGAA 7 cut(s) 56, 518, 669, 738, 909, 930, 1158
TspRI CASTG 5 cut(s) 289, 793, 913, 1096, 1171
VneI GTGCAC 1 cut(s) 1092
VpaK11BI GGWCC 1 cut(s) 1083
XapI RAATTY 1 cut(s) 668
XmaJI CCTAGG 2 cut(s) 629, 1121
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 4 cut(s) 12, 630, 1053, 1122
ZrmI AGTACT 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.