Rh2AG068000

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002379
Physical Location & Seq
Forward (+)
5657300 .. 5659759
2460 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG068000.1

Sequence Viewer

Length: 1332 bp
ATGGGTAGAGCAACGAGGTGGCTTAAGGGCTTGTTTGGGATTAAGAGGGACAAAGACAGAGAGCACCAGCAGAGACCCAATTCAGATTCAGATTCAGCTTTGGTTTCCTGTTTTGAATACAAGAAAGCTTCAAGCTTTGGGAGGGAGGCGAGTCCTCCTCCGGCGACGACTCTGCTGTGTCATAATCCGGCCACTATCCCGCCGAACATTTCACCGTCCGAGGCGGCCTGGCTGAGGTCCTTCTACAATGAAACAGAGAATGAACAGAGCAAGCACGCAATTGCAGTTGCGGCCGCCACGGCCGCCGCTGCTGATGCCGCAGTTGCTGCGGCTCAGGCGGCCGTGGCGGTCGTCCGCCTCACTAGCAATGGCAGAGGCACCATGTTCGGCGGCGGCCGCGAGAGATGGGCCGCCGTCAAGGTCCAAACTTGCTTCCGGGCATACTTGGCCAGAAAAGCCCTGAGAGCTCTGAAAGGACTGGTGAAGTTACAGGCATTAGTGAGAGGCTATTTAGTGAGGAAGCAAGCCACTGCTACACTCCACGGTATGCAAGCTCTGGTCAGAGCACAGGCCACTGTTCGTTCTCATAAATCCAGGGGCCTCAACCTCAACATTAACATCACTGAAGCCAACAGAATCGAAATGCGCGCACGAAAATCCACGGAGAGGTTTGATGAGAGTCGGAGCGAGCACACAGCTCCGAGCCACAGCAGGAGGCTCTCTGGTTCTTTGGAGAACAACAACAGTTTCAACACCATTGATGAAATCCCCAAGATTGTTGAGGTTGATACAGGAAGGCCCAAATCAAGGTCTCGCAGAACCAACACTTCCATTTCTGAACTAAGCGACGAATTTTACCAGGCAGTGTCTTCTCCACTCCCCTGTTGCTGTCCGGCCAGGTTGACCATCCCCGACCACCGGAACTTCCAAGAGTCCGACTGGGGACTCACCGGAGAGGAATGCAGGTTCTCCACGGCGCAGAGCACGCCGAGATTTGTGAACTCCTGCGGGTCTAATGCCCCGGCGACGCCTGCCAAGAGTGTCTGCGCCGACAACTTTTACAGGGGATTTGGGAACTACCCGAACTACATGGCGAGCACTCAGTCTTTCAAGGCCAAGTTGAGGTCTCACAGCGCTCCGAAACAGAGGCCGGAGCCGGGGACGAAGAAGAGGCTTTCGCTTAATGAGTTGCTCGAGTCAAGGAACAGTTTGAGTGGTGTTAAGATGCAGAGGTCTTGTTCACAAGTGCAGGAAGCCATTAATTTCAAGAATGCTGTGATGGGGAAGCTTGATAGGTCGTCAGATTTTAGTAGCTTGCTGAGGAGGTCTTAA

Protein Analysis

443

Amino Acids

48.83

Weight (kDa)

10.07

Isoelectric Point (pI)

59.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 331 - 407 2.6e-14 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 954
AccB1I GGYRCC 1 cut(s) 377
AccII CGCG 2 cut(s) 399, 648
AcoI YGGCCR 7 cut(s) 189, 291, 300, 339, 394, 447, 894
AcsI RAATTY 1 cut(s) 851
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 1028
AfeI AGCGCT 1 cut(s) 1135
AfiI CCNNNNNNNGG 6 cut(s) 234, 666, 807, 918, 1122, 1157
AflII CTTAAG 1 cut(s) 23
AgsI TTSAA 5 cut(s) 116, 132, 751, 1111, 1267
AjnI CCWGG 4 cut(s) 227, 593, 858, 896
AloI GAACNNNNNNTCC 2 cut(s) 950, 982
AluBI AGCT 8 cut(s) 98, 128, 135, 467, 554, 698, 1288, 1314
AluI AGCT 8 cut(s) 98, 128, 135, 467, 554, 698, 1288, 1314
Alw21I GWGCWC 6 cut(s) 66, 469, 568, 693, 986, 1100
Alw26I GTCTC 3 cut(s) 67, 816, 1131
AlwNI CAGNNNCTG 1 cut(s) 326
Ama87I CYCGRG 1 cut(s) 1193
Aor51HI AGCGCT 1 cut(s) 1135
ApeKI GCWGC 2 cut(s) 308, 326
ApoI RAATTY 1 cut(s) 851
AseI ATTAAT 1 cut(s) 1260
AspLEI GCGC 5 cut(s) 648, 650, 979, 1049, 1136
AspS9I GGNCC 5 cut(s) 237, 408, 421, 598, 798
AsuC2I CCSGG 3 cut(s) 437, 1022, 1158
AsuHPI GGTGA 3 cut(s) 204, 493, 940
AvaI CYCGRG 1 cut(s) 1193
AvaII GGWCC 2 cut(s) 237, 421
BalI TGGCCA 1 cut(s) 449
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 6 cut(s) 66, 469, 568, 693, 986, 1100
BbvCI CCTCAGC 2 cut(s) 233, 1319
BbvI GCAGC 2 cut(s) 295, 313
BccI CCATC 3 cut(s) 399, 914, 1273
BceAI ACGGC 4 cut(s) 315, 326, 398, 990
BcgI CGANNNNNNTGC 4 cut(s) 154, 188, 367, 401
BciT130I CCWGG 4 cut(s) 229, 595, 860, 898
BcnI CCSGG 3 cut(s) 437, 1022, 1158
BcoDI GTCTC 3 cut(s) 67, 816, 1131
BfaI CTAG 1 cut(s) 363
BfoI RGCGCY 1 cut(s) 1137
BfrI CTTAAG 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 954
BglI GCCNNNNNGGC 1 cut(s) 299
Bme1390I CCNGG 7 cut(s) 229, 437, 595, 860, 898, 1022, 1158
Bme18I GGWCC 2 cut(s) 237, 421
BmeT110I CYCGRG 1 cut(s) 1193
BmgT120I GGNCC 5 cut(s) 237, 408, 421, 598, 798
BmiI GGNNCC 3 cut(s) 379, 599, 1155
BmrFI CCNGG 7 cut(s) 229, 437, 595, 860, 898, 1022, 1158
BmrI ACTGGG 1 cut(s) 949
BmsI GCATC 2 cut(s) 304, 1215
BmuI ACTGGG 1 cut(s) 949
BpiI GAAGAC 1 cut(s) 861
BplI GAGNNNNNCTC 2 cut(s) 142, 174
Bpu10I CCTNAGC 3 cut(s) 233, 333, 1319
BpuMI CCSGG 3 cut(s) 437, 1022, 1158
BsaHI GRCGYC 1 cut(s) 1028
BsaI GGTCTC 3 cut(s) 67, 816, 1131
BsaJI CCNNGG 9 cut(s) 219, 297, 342, 541, 594, 660, 972, 1020, 1157
BsaWI WCCGGW 2 cut(s) 918, 950
Bsc4I CCNNNNNNNGG 6 cut(s) 234, 666, 807, 918, 1122, 1157
Bse1I ACTGG 2 cut(s) 483, 944
Bse3DI GCAATG 1 cut(s) 373
BseBI CCWGG 4 cut(s) 229, 595, 860, 898
BseDI CCNNGG 9 cut(s) 219, 297, 342, 541, 594, 660, 972, 1020, 1157
BseGI GGATG 1 cut(s) 906
BseLI CCNNNNNNNGG 6 cut(s) 234, 666, 807, 918, 1122, 1157
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 5 cut(s) 224, 347, 452, 1115, 1310
BseNI ACTGG 2 cut(s) 483, 944
BsePI GCGCGC 1 cut(s) 646
BseRI GAGGAG 1 cut(s) 147
BseX3I CGGCCG 4 cut(s) 291, 300, 339, 394
BseXI GCAGC 2 cut(s) 295, 313
BsgI GTGCAG 1 cut(s) 1268
Bsh1236I CGCG 2 cut(s) 399, 648
Bsh1285I CGRYCG 5 cut(s) 294, 303, 342, 351, 397
BshNI GGYRCC 1 cut(s) 377
BsiEI CGRYCG 5 cut(s) 294, 303, 342, 351, 397
BsiHKAI GWGCWC 6 cut(s) 66, 469, 568, 693, 986, 1100
BsiHKCI CYCGRG 1 cut(s) 1193
BsiSI CCGG 9 cut(s) 161, 188, 436, 893, 919, 951, 1022, 1151, 1157
BslFI GGGAC 3 cut(s) 62, 957, 1174
BslI CCNNNNNNNGG 6 cut(s) 234, 666, 807, 918, 1122, 1157
BsmAI GTCTC 3 cut(s) 67, 816, 1131
BsmFI GGGAC 3 cut(s) 62, 957, 1174
BsmI GAATGC 2 cut(s) 965, 1276
Bso31I GGTCTC 3 cut(s) 67, 816, 1131
BsoBI CYCGRG 1 cut(s) 1193
Bsp1286I GDGCHC 6 cut(s) 66, 469, 568, 693, 986, 1100
BspCNI CTCAG 5 cut(s) 225, 346, 453, 1114, 1311
BspFNI CGCG 2 cut(s) 399, 648
BspLI GGNNCC 3 cut(s) 379, 599, 1155
BspMI ACCTGC 1 cut(s) 954
BspT107I GGYRCC 1 cut(s) 377
BspTI CTTAAG 1 cut(s) 23
BspTNI GGTCTC 3 cut(s) 67, 816, 1131
BsrDI GCAATG 1 cut(s) 373
BsrI ACTGG 2 cut(s) 483, 944
BssECI CCNNGG 9 cut(s) 219, 297, 342, 541, 594, 660, 972, 1020, 1157
BssHII GCGCGC 1 cut(s) 646
BssNI GRCGYC 1 cut(s) 1028
Bst2UI CCWGG 4 cut(s) 229, 595, 860, 898
Bst4CI ACNGT 5 cut(s) 216, 545, 577, 746, 1208
Bst6I CTCTTC 1 cut(s) 1163
BstACI GRCGYC 1 cut(s) 1028
BstAFI CTTAAG 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 326
BstDEI CTNAG 6 cut(s) 233, 333, 461, 842, 1101, 1319
BstDSI CCRYGG 5 cut(s) 297, 342, 541, 660, 972
BstENI CCTNNNNNAGG 1 cut(s) 232
BstF5I GGATG 1 cut(s) 906
BstFNI CGCG 2 cut(s) 399, 648
BstH2I RGCGCY 1 cut(s) 1137
BstHHI GCGC 5 cut(s) 648, 650, 979, 1049, 1136
BstMAI GTCTC 3 cut(s) 67, 816, 1131
BstMCI CGRYCG 5 cut(s) 294, 303, 342, 351, 397
BstNI CCWGG 4 cut(s) 229, 595, 860, 898
BstSCI CCNGG 7 cut(s) 227, 435, 593, 858, 896, 1020, 1156
BstUI CGCG 2 cut(s) 399, 648
BstV1I GCAGC 2 cut(s) 295, 313
BstV2I GAAGAC 1 cut(s) 861
BstZI CGGCCG 4 cut(s) 291, 300, 339, 394
BtgI CCRYGG 5 cut(s) 297, 342, 541, 660, 972
BtsCI GGATG 1 cut(s) 906
BtsI GCAGTG 2 cut(s) 528, 870
BtsIMutI CAGTG 4 cut(s) 528, 573, 621, 870
BveI ACCTGC 1 cut(s) 954
CaiI CAGNNNCTG 1 cut(s) 326
CciNI GCGGCCGC 2 cut(s) 291, 394
CfoI GCGC 5 cut(s) 648, 650, 979, 1049, 1136
Cfr13I GGNCC 5 cut(s) 237, 408, 421, 598, 798
CseI GACGC 1 cut(s) 1036
CviAII CATG 2 cut(s) 382, 1090
DdeI CTNAG 6 cut(s) 233, 333, 461, 842, 1101, 1319
EaeI YGGCCR 7 cut(s) 189, 291, 300, 339, 394, 447, 894
EagI CGGCCG 4 cut(s) 291, 300, 339, 394
Eam1104I CTCTTC 1 cut(s) 1163
EarI CTCTTC 1 cut(s) 1163
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 467
EclXI CGGCCG 4 cut(s) 291, 300, 339, 394
Eco24I GRGCYC 1 cut(s) 469
Eco31I GGTCTC 3 cut(s) 67, 816, 1131
Eco47I GGWCC 2 cut(s) 237, 421
Eco47III AGCGCT 1 cut(s) 1135
Eco52I CGGCCG 4 cut(s) 291, 300, 339, 394
Eco53kI GAGCTC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 645
Eco88I CYCGRG 1 cut(s) 1193
EcoICRI GAGCTC 1 cut(s) 467
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoO109I RGGNCCY 2 cut(s) 237, 598
EcoRII CCWGG 4 cut(s) 227, 593, 858, 896
EcoT38I GRGCYC 1 cut(s) 469
FaeI CATG 2 cut(s) 385, 1093
FaiI YATR 6 cut(s) 183, 383, 442, 548, 588, 1091
FaqI GGGAC 3 cut(s) 62, 957, 1174
FatI CATG 2 cut(s) 381, 1089
FauI CCCGC 2 cut(s) 207, 1001
FokI GGATG 1 cut(s) 893
FriOI GRGCYC 1 cut(s) 469
FspBI CTAG 1 cut(s) 363
GlaI GCGC 5 cut(s) 647, 649, 978, 1048, 1135
HaeII RGCGCY 1 cut(s) 1137
HapII CCGG 9 cut(s) 161, 188, 436, 893, 919, 951, 1022, 1151, 1157
HgaI GACGC 1 cut(s) 1036
HhaI GCGC 5 cut(s) 648, 650, 979, 1049, 1136
Hin1I GRCGYC 1 cut(s) 1028
Hin1II CATG 2 cut(s) 385, 1093
Hin6I GCGC 5 cut(s) 646, 648, 977, 1047, 1134
HinP1I GCGC 5 cut(s) 646, 648, 977, 1047, 1134
HincII GTYRAC 1 cut(s) 903
HindII GTYRAC 1 cut(s) 903
HindIII AAGCTT 3 cut(s) 126, 133, 1286
HinfI GANTC 9 cut(s) 86, 92, 151, 169, 636, 679, 932, 945, 1196
HpaII CCGG 9 cut(s) 161, 188, 436, 893, 919, 951, 1022, 1151, 1157
HphI GGTGA 3 cut(s) 204, 493, 940
Hpy166II GTNNAC 3 cut(s) 903, 1000, 1241
Hpy188III TCNNGA 1 cut(s) 1267
Hpy8I GTNNAC 3 cut(s) 903, 1000, 1241
Hpy99I CGWCG 3 cut(s) 169, 851, 1030
HpyAV CCTTC 2 cut(s) 250, 789
HpyCH4III ACNGT 5 cut(s) 216, 545, 577, 746, 1208
HpyCH4V TGCA 5 cut(s) 284, 550, 963, 1228, 1249
HpyF3I CTNAG 6 cut(s) 233, 333, 461, 842, 1101, 1319
Hsp92I GRCGYC 1 cut(s) 1028
Hsp92II CATG 2 cut(s) 385, 1093
HspAI GCGC 5 cut(s) 646, 648, 977, 1047, 1134
LmnI GCTCC 4 cut(s) 684, 703, 1141, 1153
Lsp1109I GCAGC 2 cut(s) 295, 313
LweI GCATC 2 cut(s) 304, 1215
MaeI CTAG 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 486
MboII GAAGA 3 cut(s) 861, 1177, 1180
MfeI CAATTG 1 cut(s) 279
MhlI GDGCHC 6 cut(s) 66, 469, 568, 693, 986, 1100
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 4 cut(s) 79, 279, 851, 1261
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 6 cut(s) 160, 163, 688, 939, 941, 1205
MmeI TCCRAC 2 cut(s) 662, 960
Mox20I TGGCCA 1 cut(s) 449
MscI TGGCCA 1 cut(s) 449
MseI TTAA 7 cut(s) 24, 42, 615, 1182, 1221, 1260, 1330
Msp20I TGGCCA 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 308
MspCI CTTAAG 1 cut(s) 23
MspI CCGG 9 cut(s) 161, 188, 436, 893, 919, 951, 1022, 1151, 1157
MspR9I CCNGG 7 cut(s) 229, 437, 595, 860, 898, 1022, 1158
MunI CAATTG 1 cut(s) 279
Mva1269I GAATGC 2 cut(s) 965, 1276
MvaI CCWGG 4 cut(s) 229, 595, 860, 898
MvnI CGCG 2 cut(s) 399, 648
NciI CCSGG 3 cut(s) 437, 1022, 1158
NlaIII CATG 2 cut(s) 385, 1093
NlaIV GGNNCC 3 cut(s) 379, 599, 1155
NmeAIII GCCGAG 1 cut(s) 1014
NotI GCGGCCGC 2 cut(s) 291, 394
PaeR7I CTCGAG 1 cut(s) 1193
PauI GCGCGC 1 cut(s) 646
PctI GAATGC 2 cut(s) 965, 1276
PfeI GAWTC 3 cut(s) 86, 92, 636
PleI GAGTC 6 cut(s) 159, 163, 687, 939, 940, 1204
PpsI GAGTC 6 cut(s) 159, 163, 687, 939, 940, 1204
PpuMI RGGWCCY 1 cut(s) 237
PshBI ATTAAT 1 cut(s) 1260
Psp124BI GAGCTC 1 cut(s) 469
Psp5II RGGWCCY 1 cut(s) 237
Psp6I CCWGG 4 cut(s) 227, 593, 858, 896
PspGI CCWGG 4 cut(s) 227, 593, 858, 896
PspN4I GGNNCC 3 cut(s) 379, 599, 1155
PspPI GGNCC 5 cut(s) 237, 408, 421, 598, 798
PspPPI RGGWCCY 1 cut(s) 237
PspXI VCTCGAGB 1 cut(s) 1193
PstNI CAGNNNCTG 1 cut(s) 326
PteI GCGCGC 1 cut(s) 646
SacI GAGCTC 1 cut(s) 469
SaqAI TTAA 7 cut(s) 24, 42, 615, 1182, 1221, 1260, 1330
Sau96I GGNCC 5 cut(s) 237, 408, 421, 598, 798
SchI GAGTC 6 cut(s) 160, 163, 688, 939, 941, 1205
ScrFI CCNGG 7 cut(s) 229, 437, 595, 860, 898, 1022, 1158
SduI GDGCHC 6 cut(s) 66, 469, 568, 693, 986, 1100
SfaNI GCATC 2 cut(s) 304, 1215
SfiI GGCCNNNNNGGCC 1 cut(s) 299
Sfr274I CTCGAG 1 cut(s) 1193
SinI GGWCC 2 cut(s) 237, 421
SlaI CTCGAG 1 cut(s) 1193
SmlI CTYRAG 2 cut(s) 23, 1193
SmoI CTYRAG 2 cut(s) 23, 1193
Sse9I AATT 4 cut(s) 79, 279, 851, 1261
SspMI CTAG 1 cut(s) 363
SstI GAGCTC 1 cut(s) 469
StyD4I CCNGG 7 cut(s) 227, 435, 593, 858, 896, 1020, 1156
TaaI ACNGT 5 cut(s) 216, 545, 577, 746, 1208
TaqI TCGA 2 cut(s) 639, 1194
TasI AATT 4 cut(s) 79, 279, 851, 1261
TfiI GAWTC 3 cut(s) 86, 92, 636
Tru1I TTAA 7 cut(s) 24, 42, 615, 1182, 1221, 1260, 1330
Tru9I TTAA 7 cut(s) 24, 42, 615, 1182, 1221, 1260, 1330
TscAI CASTG 4 cut(s) 535, 580, 628, 870
TseI GCWGC 2 cut(s) 308, 326
TspDTI ATGAA 3 cut(s) 264, 276, 777
TspGWI ACGGA 1 cut(s) 677
TspRI CASTG 4 cut(s) 535, 580, 628, 870
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 2 cut(s) 237, 421
VspI ATTAAT 1 cut(s) 1260
XagI CCTNNNNNAGG 1 cut(s) 232
XapI RAATTY 1 cut(s) 851
XhoI CTCGAG 1 cut(s) 1193
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.