pycom02g05290

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002379
Physical Location & Seq
Forward (+)
3540209 .. 3541875
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g05290.1

Sequence Viewer

Length: 1296 bp
ATGGGCAGAGTAATTAGGTGGCTCAAAGGTTTGTTTGGAATAAAGACCAAAGACAATCACCAAAAACCAATTTCTGATTCCGGCGACTTCAAAGACAAGAAAGGCTCGAGCTTTGGGAGGGAGGATGCAGCCGCAACCCTGCTCTGCCACAGCCCCGCCACCATCCCGCCCAACATTTCGCCGTCCGAGGTGGCTTGGCTGAGATCCCACTACACAGAAACAGAGAAGGAGCAGAACAAGCAGGCAATTGCCGTGGCGGCTGCCACGGCCGCTGCTGCCGACGCTGCGGTTGCAGCGGCAAACGCCGCCGTGGCGGTTGTCCGCCTCACGAGTCATGGTAGAGGTACCATGTTCGGCGGCGGGAGAGAGAGATGGGCCGCTGTCAAAGTTCAAACTTGTTTCAGAGGCTATTTGGCTAGAAAAGCTCTAAGAGCTCTGAAAGGACTGGTGAAGTTGCAGGCATTAGTGAGAGGCTACTTAGTGAGGAAGCAGGCGACTGCAACTCTCCACAGTATGCAAGCTCTAATCCGAGCTCAAGCAACTGTTCGTTCTCATAAAAATCGGGGGCTGATCAGCATTGAATCCAGCAACAGATTTGAAATCCGTGCACGAAAATCCATGGAGAGATTTGGTGACAGCAGGAGTGAGCATACAGCTCCATCCCACAGCAGAAGGCTCTCTGCTTCTAATTTGGGTGACAATAATTTCAACGACATTGATGAAAGTCCCAAGATTGTTGAGGTTGATACAGGCAGCAGGCCTAAATCCAGGTCCCGGAGAACAAACACTGGTTCTGCATCCGGATTCTCCGACGACACGACTTATCAACGAGCATTGTCTTCTCCAATCCCGTATCCGGGTACTCCTGCCCGGCTATCAATCCCCGACAGCCGGAACTACCAAGACTACGACTGGGCGCTTTCCGTGGAGGAATGCAGGATGATCTCTTCGGCACAAGGCACGCCACGGTTCGTGAATTCCTGTGGCGGAGGGGCTACCAGCAATGCCGCCGGCACGCCTTCAAAGAGCGTTTGTAACGAGAATTATTTTAGAGTGTATTCGCCGAATTACATGGCGAGCACTCAGTCGTTCAAGGCGAAGTTGCGGTCTCATTCTGCTCCGAAACAGAGGCCGGAGCCGGGGGCGAAAAAGCGGCTTTCGCTGAACGAAATGTTGGCGTCGAGGAATAGTTTGAGTGGGGTTAGGATGCAGAGGTCCTGCTCGCAAGTGCAGGACGCCATTGTTTTCAAGAATGCTGTGATGGGGAAGCTTGAGAGGTCCTCTGGATTTTGGTAG

Protein Analysis

432

Amino Acids

47.05

Weight (kDa)

10.28

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 322 - 399 3e-15 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 344
AccB1I GGYRCC 1 cut(s) 344
AccIII TCCGGA 1 cut(s) 800
AclWI GGATC 1 cut(s) 198
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 1 cut(s) 976
AcyI GRCGYC 2 cut(s) 1178, 1236
AfaI GTAC 2 cut(s) 346, 862
AfiI CCNNNNNNNGG 5 cut(s) 774, 856, 857, 891, 1139
AgsI TTSAA 8 cut(s) 91, 392, 581, 599, 709, 1023, 1093, 1249
AjnI CCWGG 1 cut(s) 767
AjuI GAANNNNNNNTTGG 4 cut(s) 61, 93, 1157, 1189
AluBI AGCT 7 cut(s) 111, 425, 434, 521, 533, 656, 1270
AluI AGCT 7 cut(s) 111, 425, 434, 521, 533, 656, 1270
Alw21I GWGCWC 4 cut(s) 436, 535, 610, 1082
Alw26I GTCTC 1 cut(s) 1113
Alw44I GTGCAC 1 cut(s) 606
AlwI GGATC 1 cut(s) 198
Ama87I CYCGRG 1 cut(s) 106
Aor13HI TCCGGA 1 cut(s) 800
AoxI GGCC 4 cut(s) 267, 375, 758, 1130
ApaLI GTGCAC 1 cut(s) 606
ApeKI GCWGC 7 cut(s) 128, 260, 272, 275, 284, 293, 753
ApoI RAATTY 1 cut(s) 976
ArsI GACNNNNNNTTYG 2 cut(s) 1091, 1123
Asp718I GGTACC 1 cut(s) 344
AspLEI GCGC 1 cut(s) 919
AspS9I GGNCC 4 cut(s) 375, 771, 1215, 1278
AsuC2I CCSGG 4 cut(s) 775, 858, 871, 1140
AsuHPI GGTGA 4 cut(s) 50, 460, 644, 707
AvaI CYCGRG 1 cut(s) 106
AvaII GGWCC 3 cut(s) 771, 1215, 1278
BaeGI GKGCMC 1 cut(s) 610
BanI GGYRCC 1 cut(s) 344
BanII GRGCYC 2 cut(s) 436, 535
BauI CACGAG 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 4 cut(s) 436, 535, 610, 1082
BbvI GCAGC 7 cut(s) 140, 247, 259, 262, 271, 305, 765
BccI CCATC 4 cut(s) 170, 366, 667, 1255
BceAI ACGGC 4 cut(s) 166, 236, 282, 293
BciT130I CCWGG 1 cut(s) 769
BciVI GTATCC 1 cut(s) 864
BclI TGATCA 1 cut(s) 570
BcnI CCSGG 4 cut(s) 775, 858, 871, 1140
BcoDI GTCTC 1 cut(s) 1113
BfaI CTAG 1 cut(s) 417
BfoI RGCGCY 1 cut(s) 920
BfuI GTATCC 1 cut(s) 864
BglI GCCNNNNNGGC 2 cut(s) 257, 311
Bme1390I CCNGG 5 cut(s) 769, 775, 858, 871, 1140
Bme18I GGWCC 3 cut(s) 771, 1215, 1278
BmeT110I CYCGRG 1 cut(s) 106
BmgT120I GGNCC 4 cut(s) 375, 771, 1215, 1278
BmiI GGNNCC 3 cut(s) 346, 773, 1137
BmrFI CCNGG 5 cut(s) 769, 775, 858, 871, 1140
BmrI ACTGGG 1 cut(s) 922
BmsI GCATC 3 cut(s) 115, 806, 1197
BmuI ACTGGG 1 cut(s) 922
BpiI GAAGAC 1 cut(s) 831
BplI GAGNNNNNCTC 1 cut(s) 1265
BpuEI CTTGAG 2 cut(s) 519, 1292
BpuMI CCSGG 4 cut(s) 775, 858, 871, 1140
BsaHI GRCGYC 2 cut(s) 1178, 1236
BsaI GGTCTC 1 cut(s) 1113
BsaJI CCNNGG 8 cut(s) 186, 252, 264, 309, 618, 924, 965, 1139
BsaWI WCCGGW 1 cut(s) 800
Bsc4I CCNNNNNNNGG 5 cut(s) 774, 856, 857, 891, 1139
Bse118I RCCGGY 1 cut(s) 1010
Bse1I ACTGG 3 cut(s) 450, 793, 917
Bse3DI GCAATG 1 cut(s) 1009
BseAI TCCGGA 1 cut(s) 800
BseBI CCWGG 1 cut(s) 769
BseDI CCNNGG 8 cut(s) 186, 252, 264, 309, 618, 924, 965, 1139
BseGI GGATG 6 cut(s) 130, 162, 659, 797, 945, 1212
BseLI CCNNNNNNNGG 5 cut(s) 774, 856, 857, 891, 1139
BseMI GCAATG 1 cut(s) 1009
BseMII CTCAG 2 cut(s) 191, 1097
BseNI ACTGG 3 cut(s) 450, 793, 917
BseSI GKGCMC 1 cut(s) 610
BseX3I CGGCCG 1 cut(s) 267
BseXI GCAGC 7 cut(s) 140, 247, 259, 262, 271, 305, 765
BsgI GTGCAG 1 cut(s) 1250
Bsh1285I CGRYCG 1 cut(s) 270
BshFI GGCC 4 cut(s) 269, 377, 760, 1132
BshNI GGYRCC 1 cut(s) 344
BsiEI CGRYCG 1 cut(s) 270
BsiHKAI GWGCWC 4 cut(s) 436, 535, 610, 1082
BsiHKCI CYCGRG 1 cut(s) 106
BsiSI CCGG 9 cut(s) 81, 775, 801, 857, 871, 892, 1011, 1133, 1139
BslFI GGGAC 2 cut(s) 711, 757
BslI CCNNNNNNNGG 5 cut(s) 774, 856, 857, 891, 1139
BsmAI GTCTC 1 cut(s) 1113
BsmFI GGGAC 2 cut(s) 711, 757
BsmI GAATGC 2 cut(s) 938, 1258
BsnI GGCC 4 cut(s) 269, 377, 760, 1132
Bso31I GGTCTC 1 cut(s) 1113
BsoBI CYCGRG 1 cut(s) 106
Bsp1286I GDGCHC 4 cut(s) 436, 535, 610, 1082
Bsp13I TCCGGA 1 cut(s) 800
Bsp143I GATC 3 cut(s) 203, 570, 942
Bsp19I CCATGG 1 cut(s) 618
BspANI GGCC 4 cut(s) 269, 377, 760, 1132
BspCNI CTCAG 2 cut(s) 192, 1096
BspEI TCCGGA 1 cut(s) 800
BspLI GGNNCC 3 cut(s) 346, 773, 1137
BspPI GGATC 1 cut(s) 198
BspT107I GGYRCC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 1113
BsrDI GCAATG 1 cut(s) 1009
BsrFI RCCGGY 1 cut(s) 1010
BsrI ACTGG 3 cut(s) 450, 793, 917
BssAI RCCGGY 1 cut(s) 1010
BssECI CCNNGG 8 cut(s) 186, 252, 264, 309, 618, 924, 965, 1139
BssMI GATC 3 cut(s) 203, 570, 942
BssNI GRCGYC 2 cut(s) 1178, 1236
BssSI CACGAG 1 cut(s) 328
BssT1I CCWWGG 1 cut(s) 618
Bst2BI CACGAG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 769
Bst4CI ACNGT 3 cut(s) 512, 544, 969
Bst6I CTCTTC 1 cut(s) 952
BstACI GRCGYC 2 cut(s) 1178, 1236
BstDEI CTNAG 4 cut(s) 200, 428, 478, 1083
BstDSI CCRYGG 6 cut(s) 252, 264, 309, 618, 924, 965
BstF5I GGATG 6 cut(s) 130, 162, 659, 797, 945, 1212
BstH2I RGCGCY 1 cut(s) 920
BstHHI GCGC 1 cut(s) 919
BstKTI GATC 3 cut(s) 206, 573, 945
BstMAI GTCTC 1 cut(s) 1113
BstMBI GATC 3 cut(s) 203, 570, 942
BstMCI CGRYCG 1 cut(s) 270
BstNI CCWGG 1 cut(s) 769
BstSCI CCNGG 5 cut(s) 767, 773, 856, 869, 1138
BstSLI GKGCMC 1 cut(s) 610
BstV1I GCAGC 7 cut(s) 140, 247, 259, 262, 271, 305, 765
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 1 cut(s) 203
BstYI RGATCY 1 cut(s) 203
BstZI CGGCCG 1 cut(s) 267
BsuI GTATCC 1 cut(s) 864
BsuRI GGCC 4 cut(s) 269, 377, 760, 1132
BtgI CCRYGG 6 cut(s) 252, 264, 309, 618, 924, 965
BtsCI GGATG 6 cut(s) 130, 162, 659, 797, 945, 1212
BtsIMutI CAGTG 1 cut(s) 786
CfoI GCGC 1 cut(s) 919
Cfr10I RCCGGY 1 cut(s) 1010
Cfr13I GGNCC 4 cut(s) 375, 771, 1215, 1278
CseI GACGC 3 cut(s) 290, 1167, 1244
Csp6I GTAC 2 cut(s) 345, 861
CspCI CAANNNNNGTGG 2 cut(s) 234, 269
CviAII CATG 4 cut(s) 335, 349, 619, 1072
CviQI GTAC 2 cut(s) 345, 861
DdeI CTNAG 4 cut(s) 200, 428, 478, 1083
DpnI GATC 3 cut(s) 205, 572, 944
DpnII GATC 3 cut(s) 203, 570, 942
EaeI YGGCCR 1 cut(s) 267
EagI CGGCCG 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 952
EarI CTCTTC 1 cut(s) 952
EciI GGCGGA 2 cut(s) 311, 1002
Ecl136II GAGCTC 2 cut(s) 434, 533
EclXI CGGCCG 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 618
Eco147I AGGCCT 1 cut(s) 760
Eco24I GRGCYC 2 cut(s) 436, 535
Eco31I GGTCTC 1 cut(s) 1113
Eco47I GGWCC 3 cut(s) 771, 1215, 1278
Eco52I CGGCCG 1 cut(s) 267
Eco53kI GAGCTC 2 cut(s) 434, 533
Eco88I CYCGRG 1 cut(s) 106
EcoICRI GAGCTC 2 cut(s) 434, 533
EcoO109I RGGNCCY 3 cut(s) 771, 1215, 1278
EcoRI GAATTC 1 cut(s) 976
EcoRII CCWGG 1 cut(s) 767
EcoT14I CCWWGG 1 cut(s) 618
EcoT38I GRGCYC 2 cut(s) 436, 535
ErhI CCWWGG 1 cut(s) 618
FaeI CATG 4 cut(s) 338, 352, 622, 1075
FaiI YATR 7 cut(s) 336, 350, 515, 555, 620, 651, 1073
FaqI GGGAC 2 cut(s) 711, 757
FatI CATG 4 cut(s) 334, 348, 618, 1071
FauI CCCGC 3 cut(s) 163, 174, 353
FbaI TGATCA 1 cut(s) 570
FokI GGATG 6 cut(s) 137, 149, 646, 784, 952, 1219
FriOI GRGCYC 2 cut(s) 436, 535
FspBI CTAG 1 cut(s) 417
GlaI GCGC 1 cut(s) 918
HaeII RGCGCY 1 cut(s) 920
HaeIII GGCC 4 cut(s) 269, 377, 760, 1132
HapII CCGG 9 cut(s) 81, 775, 801, 857, 871, 892, 1011, 1133, 1139
HgaI GACGC 3 cut(s) 290, 1167, 1244
HhaI GCGC 1 cut(s) 919
Hin1I GRCGYC 2 cut(s) 1178, 1236
Hin1II CATG 4 cut(s) 338, 352, 622, 1075
Hin6I GCGC 1 cut(s) 917
HinP1I GCGC 1 cut(s) 917
HindIII AAGCTT 1 cut(s) 1268
HinfI GANTC 4 cut(s) 77, 331, 581, 804
HpaII CCGG 9 cut(s) 81, 775, 801, 857, 871, 892, 1011, 1133, 1139
HphI GGTGA 4 cut(s) 50, 460, 644, 707
Hpy166II GTNNAC 1 cut(s) 608
Hpy188I TCNGA 7 cut(s) 76, 187, 404, 438, 530, 811, 1122
Hpy188III TCNNGA 5 cut(s) 328, 801, 973, 1249, 1284
Hpy8I GTNNAC 1 cut(s) 608
Hpy99I CGWCG 3 cut(s) 284, 815, 1183
HpyAV CCTTC 3 cut(s) 220, 666, 1029
HpyCH4III ACNGT 3 cut(s) 512, 544, 969
HpyF3I CTNAG 4 cut(s) 200, 428, 478, 1083
Hsp92I GRCGYC 2 cut(s) 1178, 1236
Hsp92II CATG 4 cut(s) 338, 352, 622, 1075
HspAI GCGC 1 cut(s) 917
Kpn2I TCCGGA 1 cut(s) 800
KpnI GGTACC 1 cut(s) 348
KroI GCCGGC 1 cut(s) 1010
KroNI GCCGGC 1 cut(s) 1012
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 3 cut(s) 203, 570, 942
LmnI GCTCC 4 cut(s) 229, 661, 1123, 1135
Lsp1109I GCAGC 7 cut(s) 140, 247, 259, 262, 271, 305, 765
LweI GCATC 3 cut(s) 115, 806, 1197
MaeI CTAG 1 cut(s) 417
MaeIII GTNAC 3 cut(s) 632, 695, 1034
MalI GATC 3 cut(s) 205, 572, 944
MboI GATC 3 cut(s) 203, 570, 942
MboII GAAGA 2 cut(s) 831, 939
MfeI CAATTG 1 cut(s) 246
MflI RGATCY 1 cut(s) 203
MhlI GDGCHC 4 cut(s) 436, 535, 610, 1082
MluCI AATT 8 cut(s) 12, 69, 246, 688, 703, 976, 1042, 1066
MlyI GAGTC 1 cut(s) 340
MmeI TCCRAC 1 cut(s) 834
MroI TCCGGA 1 cut(s) 800
MroNI GCCGGC 1 cut(s) 1010
MspA1I CMGCKG 3 cut(s) 272, 296, 380
MspI CCGG 9 cut(s) 81, 775, 801, 857, 871, 892, 1011, 1133, 1139
MspR9I CCNGG 5 cut(s) 769, 775, 858, 871, 1140
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 2 cut(s) 938, 1258
MvaI CCWGG 1 cut(s) 769
NaeI GCCGGC 1 cut(s) 1012
NciI CCSGG 4 cut(s) 775, 858, 871, 1140
NcoI CCATGG 1 cut(s) 618
NdeII GATC 3 cut(s) 203, 570, 942
NgoMIV GCCGGC 1 cut(s) 1010
NlaIII CATG 4 cut(s) 338, 352, 622, 1075
NlaIV GGNNCC 3 cut(s) 346, 773, 1137
NmuCI GTSAC 2 cut(s) 632, 695
PaeR7I CTCGAG 1 cut(s) 106
PceI AGGCCT 1 cut(s) 760
PcsI WCGNNNNNNNCGW 1 cut(s) 1094
PctI GAATGC 2 cut(s) 938, 1258
PdiI GCCGGC 1 cut(s) 1012
PfeI GAWTC 3 cut(s) 77, 581, 804
PfoI TCCNGGA 1 cut(s) 773
PleI GAGTC 1 cut(s) 339
PpsI GAGTC 1 cut(s) 339
PpuMI RGGWCCY 3 cut(s) 771, 1215, 1278
Psp124BI GAGCTC 2 cut(s) 436, 535
Psp5II RGGWCCY 3 cut(s) 771, 1215, 1278
Psp6I CCWGG 1 cut(s) 767
PspGI CCWGG 1 cut(s) 767
PspN4I GGNNCC 3 cut(s) 346, 773, 1137
PspPI GGNCC 4 cut(s) 375, 771, 1215, 1278
PspPPI RGGWCCY 3 cut(s) 771, 1215, 1278
PspXI VCTCGAGB 1 cut(s) 106
PsuI RGATCY 1 cut(s) 203
RsaI GTAC 2 cut(s) 346, 862
RsaNI GTAC 2 cut(s) 345, 861
SacI GAGCTC 2 cut(s) 436, 535
Sau3AI GATC 3 cut(s) 203, 570, 942
Sau96I GGNCC 4 cut(s) 375, 771, 1215, 1278
SchI GAGTC 1 cut(s) 340
ScrFI CCNGG 5 cut(s) 769, 775, 858, 871, 1140
SduI GDGCHC 4 cut(s) 436, 535, 610, 1082
SfaNI GCATC 3 cut(s) 115, 806, 1197
Sfr274I CTCGAG 1 cut(s) 106
SinI GGWCC 3 cut(s) 771, 1215, 1278
SlaI CTCGAG 1 cut(s) 106
SmlI CTYRAG 3 cut(s) 106, 534, 1271
SmoI CTYRAG 3 cut(s) 106, 534, 1271
Sse9I AATT 8 cut(s) 12, 69, 246, 688, 703, 976, 1042, 1066
SseBI AGGCCT 1 cut(s) 760
SspMI CTAG 1 cut(s) 417
SstI GAGCTC 2 cut(s) 436, 535
StuI AGGCCT 1 cut(s) 760
StyD4I CCNGG 5 cut(s) 767, 773, 856, 869, 1138
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 3 cut(s) 512, 544, 969
TaqI TCGA 2 cut(s) 107, 1181
TasI AATT 8 cut(s) 12, 69, 246, 688, 703, 976, 1042, 1066
TauI GCSGC 9 cut(s) 134, 260, 272, 299, 308, 360, 380, 1010, 1156
TfiI GAWTC 3 cut(s) 77, 581, 804
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 2 cut(s) 632, 695
TseI GCWGC 7 cut(s) 128, 260, 272, 275, 284, 293, 753
Tsp45I GTSAC 2 cut(s) 632, 695
TspDTI ATGAA 1 cut(s) 735
TspGWI ACGGA 2 cut(s) 593, 913
TspRI CASTG 1 cut(s) 793
VneI GTGCAC 1 cut(s) 606
VpaK11BI GGWCC 3 cut(s) 771, 1215, 1278
XapI RAATTY 1 cut(s) 976
XhoI CTCGAG 1 cut(s) 106
XspI CTAG 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.