MD09G1201900.v1.1

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002379
Physical Location & Seq
Reverse (-)
18653548 .. 18655443
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1201900.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGAAGAGAGAGAAACACCACGTCGACAACACCAGCACCGTGTCCAGCGACCGGAAGGAGAAGAAAAGGTGGAGCTTTGCCAAGTCAGGGAAAGACACTACCACCAATCTTCCGCCGAATGTTCCTGTCCCTGCTGACTCTGCCTGGCTCAGATCCTATCTTGCCGAGTCGGAGAAGGAGCAGAACAAGCACGCAATTGCAGTGGCTGCGGCCACCGCAGCCGCTGCTGATGCCGCCGTGGCTGCCGCACAGGCGGCTGTGGCTGTTGTGAGGTTGACAAGTCAAGGCGGCCATGGCGGTGGAGGCGGGATCATGTTTGGGAAAAGAGAGAGATGGGCTGCAATGAAGATTCAAACAGTTTTTAGAGGCTATTTGGCGAGGAAGGCGCACCGTGCTCTTAGAGGACTGGTTAAGTTGCAGGCTCTTGTTAGAGGATTTCTTGTCCGGAAGCGGGCTGCTGCTACTCTTTACAGTATGCAAGCTCTTTTGAGAGCACAGACTACTGTCCGATACCAGCGTGCGCGTCGTTCTTTCAATAAAGAGAATAGGTTCCTGCCCGAAATCAATGCCCGGAAGTGTGTTGAAAGATTTGAAGATGCAAGAAGTGAATTTTATAGCAGGAGGCTATCTGCAACTTATGAAACTTCGGTTGATAGCTTTGATGAAAGCCCGAAAATTGTGGAAATTGACACATTCAAGCCGAGATCCAGGTCTCGTAGATTCAACACCATATTATCTGATCGTGGCGAAGACCTGCATTACCAAACAATCTCCTCACCGCTGCCCTGTCCAATGCCAACTCGTATCTCCATACCTGATAGCCAGAAACCTCAAGATTTTGAATGGTACTTCAATGGTGATGAATGCAAGTTCTTGACTGCCCATAACACTCCGCGGTTTGCCACCTCTTTTCGTTCTAATGCTCCTGGCACACCATCTAAAAGTGTTTGTGGAGACAGCTTCTTTCGGCCTTACTCAAACTTCCCAAACTACATGTCAAGCACTCAGTCCTTTAATGCCAAGTCGAGATCCCACAGCGCCCCGAAGCAAAGGCCGGAACCAGGGCCTAAGAAGAGGCTTTCGCTTAACGAAATGATGGCAGCCAGAAACAGCATCAGCAGCATTCGAATGCATCGATCGTGCAATCAAGTCCAAGAAGAAGAAGAAGAAGAAGAAGAAGTCTCGAATGATTGA

Protein Analysis

398

Amino Acids

44.78

Weight (kDa)

9.93

Isoelectric Point (pI)

65.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 298 - 377 6.1e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 762
AccI GTMKAC 1 cut(s) 24
AccII CGCG 2 cut(s) 523, 895
AccIII TCCGGA 1 cut(s) 444
AclWI GGATC 4 cut(s) 147, 317, 699, 1023
AcoI YGGCCR 2 cut(s) 210, 289
AcsI RAATTY 1 cut(s) 608
AfaI GTAC 1 cut(s) 848
AfiI CCNNNNNNNGG 4 cut(s) 51, 87, 451, 1061
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 8 cut(s) 353, 535, 584, 593, 697, 724, 842, 853
AjiI CACGTC 1 cut(s) 22
AjnI CCWGG 4 cut(s) 143, 707, 925, 1060
AluBI AGCT 4 cut(s) 75, 482, 657, 960
AluI AGCT 4 cut(s) 75, 482, 657, 960
Alw21I GWGCWC 2 cut(s) 397, 496
Alw26I GTCTC 3 cut(s) 717, 948, 1186
AlwI GGATC 4 cut(s) 147, 317, 699, 1023
AlwNI CAGNNNCTG 2 cut(s) 206, 224
Aor13HI TCCGGA 1 cut(s) 444
AoxI GGCC 5 cut(s) 210, 289, 968, 1052, 1064
ApoI RAATTY 1 cut(s) 608
ArsI GACNNNNNNTTYG 2 cut(s) 980, 1012
Asp700I GAANNNNTTC 1 cut(s) 548
AspLEI GCGC 3 cut(s) 388, 523, 1040
AspS9I GGNCC 1 cut(s) 1064
AsuC2I CCSGG 1 cut(s) 571
AsuHPI GGTGA 2 cut(s) 768, 869
AsuII TTCGAA 1 cut(s) 1126
BaeI ACNNNNGTAYC 2 cut(s) 502, 535
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 2 cut(s) 397, 496
BccI CCATC 3 cut(s) 327, 943, 1090
BceAI ACGGC 1 cut(s) 221
BcgI CGANNNNNNTGC 2 cut(s) 548, 582
BciT130I CCWGG 4 cut(s) 145, 709, 927, 1062
BcnI CCSGG 1 cut(s) 571
BcoDI GTCTC 3 cut(s) 717, 948, 1186
BfoI RGCGCY 1 cut(s) 1041
BfuAI ACCTGC 1 cut(s) 762
BglI GCCNNNNNGGC 2 cut(s) 239, 251
Bme1390I CCNGG 5 cut(s) 145, 571, 709, 927, 1062
BmgBI CACGTC 1 cut(s) 22
BmgT120I GGNCC 1 cut(s) 1064
BmiI GGNNCC 2 cut(s) 551, 1059
BmrFI CCNGG 5 cut(s) 145, 571, 709, 927, 1062
BmsI GCATC 4 cut(s) 220, 586, 1122, 1141
BoxI GACNNNNGTC 1 cut(s) 503
BpiI GAAGAC 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 1126
BpuEI CTTGAG 1 cut(s) 816
BpuMI CCSGG 1 cut(s) 571
Bsa29I ATCGAT 1 cut(s) 1135
BsaI GGTCTC 1 cut(s) 717
BsaJI CCNNGG 4 cut(s) 237, 292, 893, 1061
BsaWI WCCGGW 2 cut(s) 51, 444
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 87, 451, 1061
Bse1I ACTGG 1 cut(s) 411
Bse3DI GCAATG 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 444
BseBI CCWGG 4 cut(s) 145, 709, 927, 1062
BseCI ATCGAT 1 cut(s) 1135
BseDI CCNNGG 4 cut(s) 237, 292, 893, 1061
BseLI CCNNNNNNNGG 4 cut(s) 51, 87, 451, 1061
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 2 cut(s) 163, 1019
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 763
Bsh1236I CGCG 2 cut(s) 523, 895
Bsh1285I CGRYCG 2 cut(s) 52, 1139
BshFI GGCC 5 cut(s) 212, 291, 970, 1054, 1066
BshVI ATCGAT 1 cut(s) 1135
BsiEI CGRYCG 2 cut(s) 52, 1139
BsiHKAI GWGCWC 2 cut(s) 397, 496
BsiSI CCGG 4 cut(s) 52, 445, 571, 1055
BslFI GGGAC 1 cut(s) 113
BslI CCNNNNNNNGG 4 cut(s) 51, 87, 451, 1061
BsmAI GTCTC 3 cut(s) 717, 948, 1186
BsmFI GGGAC 1 cut(s) 113
BsmI GAATGC 3 cut(s) 869, 1122, 1134
BsnI GGCC 5 cut(s) 212, 291, 970, 1054, 1066
Bso31I GGTCTC 1 cut(s) 717
Bsp119I TTCGAA 1 cut(s) 1126
Bsp1286I GDGCHC 2 cut(s) 397, 496
Bsp13I TCCGGA 1 cut(s) 444
Bsp143I GATC 6 cut(s) 152, 309, 704, 739, 1028, 1136
Bsp19I CCATGG 1 cut(s) 292
BspANI GGCC 5 cut(s) 212, 291, 970, 1054, 1066
BspCNI CTCAG 2 cut(s) 162, 1018
BspDI ATCGAT 1 cut(s) 1135
BspEI TCCGGA 1 cut(s) 444
BspFNI CGCG 2 cut(s) 523, 895
BspLI GGNNCC 2 cut(s) 551, 1059
BspMI ACCTGC 1 cut(s) 762
BspPI GGATC 4 cut(s) 147, 317, 699, 1023
BspT104I TTCGAA 1 cut(s) 1126
BspTNI GGTCTC 1 cut(s) 717
BsrDI GCAATG 1 cut(s) 348
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 4 cut(s) 237, 292, 893, 1061
BssMI GATC 6 cut(s) 152, 309, 704, 739, 1028, 1136
BssT1I CCWWGG 1 cut(s) 292
Bst2UI CCWGG 4 cut(s) 145, 709, 927, 1062
Bst4CI ACNGT 5 cut(s) 40, 358, 392, 473, 505
Bst6I CTCTTC 1 cut(s) 1067
BstAPI GCANNNNNTGC 2 cut(s) 206, 224
BstBI TTCGAA 1 cut(s) 1126
BstC8I GCNNGC 5 cut(s) 192, 420, 453, 480, 519
BstDEI CTNAG 4 cut(s) 149, 398, 1005, 1068
BstDSI CCRYGG 3 cut(s) 237, 292, 893
BstFNI CGCG 2 cut(s) 523, 895
BstH2I RGCGCY 1 cut(s) 1041
BstHHI GCGC 3 cut(s) 388, 523, 1040
BstKTI GATC 6 cut(s) 155, 312, 707, 742, 1031, 1139
BstMAI GTCTC 3 cut(s) 717, 948, 1186
BstMBI GATC 6 cut(s) 152, 309, 704, 739, 1028, 1136
BstMCI CGRYCG 2 cut(s) 52, 1139
BstNI CCWGG 4 cut(s) 145, 709, 927, 1062
BstNSI RCATGY 1 cut(s) 997
BstPAI GACNNNNGTC 1 cut(s) 503
BstSCI CCNGG 5 cut(s) 143, 569, 707, 925, 1060
BstUI CGCG 2 cut(s) 523, 895
BstV2I GAAGAC 1 cut(s) 756
BstX2I RGATCY 3 cut(s) 152, 704, 1028
BstXI CCANNNNNNTGG 1 cut(s) 299
BstYI RGATCY 3 cut(s) 152, 704, 1028
Bsu15I ATCGAT 1 cut(s) 1135
BsuRI GGCC 5 cut(s) 212, 291, 970, 1054, 1066
BsuTUI ATCGAT 1 cut(s) 1135
BtgI CCRYGG 3 cut(s) 237, 292, 893
BtrI CACGTC 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
BveI ACCTGC 1 cut(s) 762
Cac8I GCNNGC 5 cut(s) 192, 420, 453, 480, 519
CaiI CAGNNNCTG 2 cut(s) 206, 224
CfoI GCGC 3 cut(s) 388, 523, 1040
Cfr13I GGNCC 1 cut(s) 1064
Cfr42I CCGCGG 1 cut(s) 896
ClaI ATCGAT 1 cut(s) 1135
CseI GACGC 1 cut(s) 512
Csp6I GTAC 1 cut(s) 847
CspCI CAANNNNNGTGG 2 cut(s) 183, 218
CviAII CATG 3 cut(s) 293, 313, 994
CviQI GTAC 1 cut(s) 847
DdeI CTNAG 4 cut(s) 149, 398, 1005, 1068
DpnI GATC 6 cut(s) 154, 311, 706, 741, 1030, 1138
DpnII GATC 6 cut(s) 152, 309, 704, 739, 1028, 1136
EaeI YGGCCR 2 cut(s) 210, 289
Eam1104I CTCTTC 1 cut(s) 1067
EarI CTCTTC 1 cut(s) 1067
EciI GGCGGA 1 cut(s) 102
Eco130I CCWWGG 1 cut(s) 292
Eco31I GGTCTC 1 cut(s) 717
EcoO109I RGGNCCY 1 cut(s) 1064
EcoRII CCWGG 4 cut(s) 143, 707, 925, 1060
EcoT14I CCWWGG 1 cut(s) 292
EcoT22I ATGCAT 1 cut(s) 1134
ErhI CCWWGG 1 cut(s) 292
FaeI CATG 3 cut(s) 296, 316, 997
FaiI YATR 9 cut(s) 294, 314, 476, 615, 639, 731, 812, 885, 995
FaqI GGGAC 1 cut(s) 113
FatI CATG 3 cut(s) 292, 312, 993
FauI CCCGC 2 cut(s) 299, 444
FblI GTMKAC 1 cut(s) 24
GlaI GCGC 3 cut(s) 387, 522, 1039
HaeII RGCGCY 1 cut(s) 1041
HaeIII GGCC 5 cut(s) 212, 291, 970, 1054, 1066
HapII CCGG 4 cut(s) 52, 445, 571, 1055
HgaI GACGC 1 cut(s) 512
HhaI GCGC 3 cut(s) 388, 523, 1040
Hin1II CATG 3 cut(s) 296, 316, 997
Hin6I GCGC 3 cut(s) 386, 521, 1038
HinP1I GCGC 3 cut(s) 386, 521, 1038
HincII GTYRAC 2 cut(s) 25, 276
HindII GTYRAC 2 cut(s) 25, 276
HinfI GANTC 4 cut(s) 137, 167, 349, 720
HpaII CCGG 4 cut(s) 52, 445, 571, 1055
HphI GGTGA 2 cut(s) 768, 869
Hpy166II GTNNAC 2 cut(s) 25, 276
Hpy188I TCNGA 4 cut(s) 152, 172, 509, 739
Hpy188III TCNNGA 5 cut(s) 445, 833, 874, 1026, 1183
Hpy8I GTNNAC 2 cut(s) 25, 276
Hpy99I CGWCG 2 cut(s) 26, 528
HpyAV CCTTC 3 cut(s) 49, 169, 376
HpyCH4III ACNGT 5 cut(s) 40, 358, 392, 473, 505
HpyCH4IV ACGT 1 cut(s) 21
HpyF3I CTNAG 4 cut(s) 149, 398, 1005, 1068
HpySE526I ACGT 1 cut(s) 21
Hsp92II CATG 3 cut(s) 296, 316, 997
HspAI GCGC 3 cut(s) 386, 521, 1038
Kpn2I TCCGGA 1 cut(s) 444
KspI CCGCGG 1 cut(s) 896
Kzo9I GATC 6 cut(s) 152, 309, 704, 739, 1028, 1136
LmnI GCTCC 3 cut(s) 72, 178, 928
LweI GCATC 4 cut(s) 220, 586, 1122, 1141
MaeII ACGT 1 cut(s) 21
MalI GATC 6 cut(s) 154, 311, 706, 741, 1030, 1138
MboI GATC 6 cut(s) 152, 309, 704, 739, 1028, 1136
MfeI CAATTG 1 cut(s) 195
MflI RGATCY 3 cut(s) 152, 704, 1028
MhlI GDGCHC 2 cut(s) 397, 496
MluCI AATT 4 cut(s) 195, 608, 675, 684
MlyI GAGTC 2 cut(s) 131, 176
MmeI TCCRAC 1 cut(s) 150
Mph1103I ATGCAT 1 cut(s) 1134
MroI TCCGGA 1 cut(s) 444
MroXI GAANNNNTTC 1 cut(s) 548
MseI TTAA 3 cut(s) 411, 1014, 1086
MslI CAYNNNNRTG 2 cut(s) 297, 1127
MspA1I CMGCKG 3 cut(s) 224, 781, 895
MspI CCGG 4 cut(s) 52, 445, 571, 1055
MspR9I CCNGG 5 cut(s) 145, 571, 709, 927, 1062
MunI CAATTG 1 cut(s) 195
Mva1269I GAATGC 3 cut(s) 869, 1122, 1134
MvaI CCWGG 4 cut(s) 145, 709, 927, 1062
MvnI CGCG 2 cut(s) 523, 895
NciI CCSGG 1 cut(s) 571
NcoI CCATGG 1 cut(s) 292
NdeII GATC 6 cut(s) 152, 309, 704, 739, 1028, 1136
NlaIII CATG 3 cut(s) 296, 316, 997
NlaIV GGNNCC 2 cut(s) 551, 1059
NmeAIII GCCGAG 2 cut(s) 190, 726
NsiI ATGCAT 1 cut(s) 1134
NspI RCATGY 1 cut(s) 997
NspV TTCGAA 1 cut(s) 1126
PciI ACATGT 1 cut(s) 993
PcsI WCGNNNNNNNCGW 1 cut(s) 1132
PctI GAATGC 3 cut(s) 869, 1122, 1134
PdmI GAANNNNTTC 1 cut(s) 548
PfeI GAWTC 2 cut(s) 349, 720
Ple19I CGATCG 1 cut(s) 1139
PleI GAGTC 2 cut(s) 131, 175
PpsI GAGTC 2 cut(s) 131, 175
PscI ACATGT 1 cut(s) 993
PshAI GACNNNNGTC 1 cut(s) 503
Psp6I CCWGG 4 cut(s) 143, 707, 925, 1060
PspGI CCWGG 4 cut(s) 143, 707, 925, 1060
PspN4I GGNNCC 2 cut(s) 551, 1059
PspPI GGNCC 1 cut(s) 1064
PstNI CAGNNNCTG 2 cut(s) 206, 224
PsuI RGATCY 3 cut(s) 152, 704, 1028
PvuI CGATCG 1 cut(s) 1139
RsaI GTAC 1 cut(s) 848
RsaNI GTAC 1 cut(s) 847
RseI CAYNNNNRTG 2 cut(s) 297, 1127
SacII CCGCGG 1 cut(s) 896
SalI GTCGAC 1 cut(s) 23
SaqAI TTAA 3 cut(s) 411, 1014, 1086
Sau3AI GATC 6 cut(s) 152, 309, 704, 739, 1028, 1136
Sau96I GGNCC 1 cut(s) 1064
SchI GAGTC 2 cut(s) 131, 176
ScrFI CCNGG 5 cut(s) 145, 571, 709, 927, 1062
SduI GDGCHC 2 cut(s) 397, 496
SfaNI GCATC 4 cut(s) 220, 586, 1122, 1141
Sfr303I CCGCGG 1 cut(s) 896
SfuI TTCGAA 1 cut(s) 1126
SgrBI CCGCGG 1 cut(s) 896
SmiMI CAYNNNNRTG 2 cut(s) 297, 1127
SmlI CTYRAG 1 cut(s) 831
SmoI CTYRAG 1 cut(s) 831
Sse9I AATT 4 cut(s) 195, 608, 675, 684
StyD4I CCNGG 5 cut(s) 143, 569, 707, 925, 1060
StyI CCWWGG 1 cut(s) 292
TaaI ACNGT 5 cut(s) 40, 358, 392, 473, 505
TaiI ACGT 1 cut(s) 24
TaqI TCGA 5 cut(s) 24, 1025, 1126, 1135, 1184
TasI AATT 4 cut(s) 195, 608, 675, 684
TauI GCSGC 6 cut(s) 212, 224, 236, 248, 257, 291
TfiI GAWTC 2 cut(s) 349, 720
Tru1I TTAA 3 cut(s) 411, 1014, 1086
Tru9I TTAA 3 cut(s) 411, 1014, 1086
TscAI CASTG 1 cut(s) 207
TspDTI ATGAA 5 cut(s) 17, 359, 654, 678, 876
TspRI CASTG 1 cut(s) 207
XapI RAATTY 1 cut(s) 608
XceI RCATGY 1 cut(s) 997
XmiI GTMKAC 1 cut(s) 24
XmnI GAANNNNTTC 1 cut(s) 548
Zsp2I ATGCAT 1 cut(s) 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.