Rh2BG067400

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0002379
Physical Location & Seq
Forward (+)
5116323 .. 5118799
2477 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG067400.1

Sequence Viewer

Length: 1329 bp
ATGGGTAGAGCAACGAGGTGGCTTAAGGGCTTGTTTGGGATTAAGAGGGACAAAGACAGAGAGCACCAGCAGAGACCCAATTCAGATTCAGATTCAGCTTTGGTTTCCTGTTTTGAATACAAGAAAGCTTCAAGCTTTGGGAGGGAGGCGAGTCCTCCTCCGGCGACGACTCTGCTGTGTCATAATCCGGCCACTATCCCGCCGAACATTTCACCGTCCGAGGCGGCCTGGCTGAGGTCCTTCTACAATGAAACAGAGAATGAACAGAGCAAGCACGCAATCGCAGTTGCGGCCGCCACTGCCGCCGCTGCTGATGCCGCAGTTGCTGCGGCTCAGGCGGCCGTGGCGGTCGTCCGCCTCACTAGCAATGGCAGAGGCACCATGTTCGGCGGCGGCCGGGAGAGATGGGCCGCCGTCAAGGTCCAAACTTGCTTCCGGGCATACTTGGCCAGAAAAGCCCTGAGAGCTCTGAAAGGACTGGTGAAGTTACAGGCATTAGTGAGAGGCTATTTAGTGAGGAAGCAAGCCACTGCTACACTCCACGGTATGCAAGCTCTGGTCAGAGCACAGGCCACTGTTCGTTCTCATAAATCCAGGGGCCTCAACCTCAACATTAACATCACTGAAGCCAACAGAATCGAAATGCGCGCACGAAAATCCACGGAGAGGTTTGATGAGAGTCGGAGCGAGCCAGCTCCGAGCCACAGCAGGAGGCTCTCTGGTTGTTTGGAGAACAACAACAGTTTCAACACCATTGATGAAATTCCCAAGATTGTTGAGGTTGATACAGGAAGGCCCAAATCCAGGTCTCGCAGAACCAACACTTCCATTTCTGAACTAAGCGACGAATTTTACCAGGCAGTGTCTTCTCCACTCCCCTGTTGCTGTCCGGCCAGGTTGACCATCCCCGACCACCGGAGCTTCCAAGAGTCCGACTGGGGACTCACCGGAGAGGAATGCAGGTTCTCCACGGCGCAGAGCACGCCGAGATTTGTGAACTCCTGCGGGTCTAATGCCCCGGCGACGCCGGCCAAGAGTGTCTGCGCCGACAACTTTTACAGGGGATTTGGGAACTACCCGAACTACATGGCGAGCACTCAGTCTTTCAAGGCCAAGTTGAGGTCTCACAGCGCTCCGAAACAGAGGCCGGAGCCGGGGACGAAGAAGAGGCTTTCGCTTAATGAGTTGCTCGAGTCAAGGAACAGTTTGAGTGGTGTTAAGATGCAGAGGTCTTGTTCACAAGTGCAGGAAGCCATTAATTTCAAGAATGCTGTGATGGGGAAGCTTGATAGGTCGTCAGATTTTAGTAGCTTGCTGAGGAGGTCTTAA

Protein Analysis

442

Amino Acids

48.68

Weight (kDa)

10.03

Isoelectric Point (pI)

62.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 330 - 406 2.6e-14 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 951
AccB1I GGYRCC 1 cut(s) 377
AccII CGCG 1 cut(s) 648
AcoI YGGCCR 7 cut(s) 189, 291, 339, 394, 447, 891, 1029
AcsI RAATTY 2 cut(s) 762, 848
AcuI CTGAAG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 1025
AfeI AGCGCT 1 cut(s) 1132
AfiI CCNNNNNNNGG 6 cut(s) 234, 666, 804, 915, 1119, 1154
AflII CTTAAG 1 cut(s) 23
AgsI TTSAA 5 cut(s) 116, 132, 748, 1108, 1264
AjnI CCWGG 5 cut(s) 227, 593, 803, 855, 893
AloI GAACNNNNNNTCC 2 cut(s) 947, 979
AluBI AGCT 9 cut(s) 98, 128, 135, 467, 554, 695, 921, 1285, 1311
AluI AGCT 9 cut(s) 98, 128, 135, 467, 554, 695, 921, 1285, 1311
Alw21I GWGCWC 5 cut(s) 66, 469, 568, 983, 1097
Alw26I GTCTC 3 cut(s) 67, 813, 1128
AlwNI CAGNNNCTG 1 cut(s) 326
Ama87I CYCGRG 1 cut(s) 1190
Aor51HI AGCGCT 1 cut(s) 1132
ApeKI GCWGC 2 cut(s) 308, 326
ApoI RAATTY 2 cut(s) 762, 848
AseI ATTAAT 1 cut(s) 1257
AspLEI GCGC 5 cut(s) 648, 650, 976, 1046, 1133
AspS9I GGNCC 5 cut(s) 237, 408, 421, 598, 795
AsuC2I CCSGG 4 cut(s) 398, 437, 1019, 1155
AsuHPI GGTGA 3 cut(s) 204, 493, 937
AvaI CYCGRG 1 cut(s) 1190
AvaII GGWCC 2 cut(s) 237, 421
BalI TGGCCA 1 cut(s) 449
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 469
BbsI GAAGAC 1 cut(s) 858
Bbv12I GWGCWC 5 cut(s) 66, 469, 568, 983, 1097
BbvCI CCTCAGC 2 cut(s) 233, 1316
BbvI GCAGC 2 cut(s) 295, 313
BccI CCATC 3 cut(s) 399, 911, 1270
BceAI ACGGC 3 cut(s) 326, 398, 987
BcgI CGANNNNNNTGC 4 cut(s) 154, 188, 367, 401
BciT130I CCWGG 5 cut(s) 229, 595, 805, 857, 895
BcnI CCSGG 4 cut(s) 398, 437, 1019, 1155
BcoDI GTCTC 3 cut(s) 67, 813, 1128
BfaI CTAG 1 cut(s) 363
BfoI RGCGCY 1 cut(s) 1134
BfrI CTTAAG 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 951
Bme1390I CCNGG 9 cut(s) 229, 398, 437, 595, 805, 857, 895, 1019, 1155
Bme18I GGWCC 2 cut(s) 237, 421
BmeT110I CYCGRG 1 cut(s) 1190
BmgT120I GGNCC 5 cut(s) 237, 408, 421, 598, 795
BmiI GGNNCC 3 cut(s) 379, 599, 1152
BmrFI CCNGG 9 cut(s) 229, 398, 437, 595, 805, 857, 895, 1019, 1155
BmrI ACTGGG 1 cut(s) 946
BmsI GCATC 2 cut(s) 304, 1212
BmuI ACTGGG 1 cut(s) 946
BpiI GAAGAC 1 cut(s) 858
BplI GAGNNNNNCTC 2 cut(s) 142, 174
Bpu10I CCTNAGC 3 cut(s) 233, 333, 1316
BpuMI CCSGG 4 cut(s) 398, 437, 1019, 1155
BsaHI GRCGYC 1 cut(s) 1025
BsaI GGTCTC 3 cut(s) 67, 813, 1128
BsaJI CCNNGG 8 cut(s) 219, 342, 541, 594, 660, 969, 1017, 1154
BsaWI WCCGGW 2 cut(s) 915, 947
Bsc4I CCNNNNNNNGG 6 cut(s) 234, 666, 804, 915, 1119, 1154
Bse118I RCCGGY 1 cut(s) 1027
Bse1I ACTGG 2 cut(s) 483, 941
Bse3DI GCAATG 1 cut(s) 373
BseBI CCWGG 5 cut(s) 229, 595, 805, 857, 895
BseDI CCNNGG 8 cut(s) 219, 342, 541, 594, 660, 969, 1017, 1154
BseGI GGATG 1 cut(s) 903
BseLI CCNNNNNNNGG 6 cut(s) 234, 666, 804, 915, 1119, 1154
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 5 cut(s) 224, 347, 452, 1112, 1307
BseNI ACTGG 2 cut(s) 483, 941
BsePI GCGCGC 1 cut(s) 646
BseRI GAGGAG 1 cut(s) 147
BseX3I CGGCCG 3 cut(s) 291, 339, 394
BseXI GCAGC 2 cut(s) 295, 313
BsgI GTGCAG 1 cut(s) 1265
Bsh1236I CGCG 1 cut(s) 648
Bsh1285I CGRYCG 4 cut(s) 294, 342, 351, 397
BshNI GGYRCC 1 cut(s) 377
BsiEI CGRYCG 4 cut(s) 294, 342, 351, 397
BsiHKAI GWGCWC 5 cut(s) 66, 469, 568, 983, 1097
BsiHKCI CYCGRG 1 cut(s) 1190
BslFI GGGAC 3 cut(s) 62, 954, 1171
BslI CCNNNNNNNGG 6 cut(s) 234, 666, 804, 915, 1119, 1154
BsmAI GTCTC 3 cut(s) 67, 813, 1128
BsmFI GGGAC 3 cut(s) 62, 954, 1171
BsmI GAATGC 2 cut(s) 962, 1273
Bso31I GGTCTC 3 cut(s) 67, 813, 1128
BsoBI CYCGRG 1 cut(s) 1190
Bsp1286I GDGCHC 5 cut(s) 66, 469, 568, 983, 1097
BspCNI CTCAG 5 cut(s) 225, 346, 453, 1111, 1308
BspFNI CGCG 1 cut(s) 648
BspLI GGNNCC 3 cut(s) 379, 599, 1152
BspMI ACCTGC 1 cut(s) 951
BspT107I GGYRCC 1 cut(s) 377
BspTI CTTAAG 1 cut(s) 23
BspTNI GGTCTC 3 cut(s) 67, 813, 1128
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 1 cut(s) 1027
BsrI ACTGG 2 cut(s) 483, 941
BssAI RCCGGY 1 cut(s) 1027
BssECI CCNNGG 8 cut(s) 219, 342, 541, 594, 660, 969, 1017, 1154
BssHII GCGCGC 1 cut(s) 646
BssNI GRCGYC 1 cut(s) 1025
Bst2UI CCWGG 5 cut(s) 229, 595, 805, 857, 895
Bst4CI ACNGT 5 cut(s) 216, 545, 577, 743, 1205
Bst6I CTCTTC 1 cut(s) 1160
BstACI GRCGYC 1 cut(s) 1025
BstAFI CTTAAG 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 326
BstDEI CTNAG 6 cut(s) 233, 333, 461, 839, 1098, 1316
BstDSI CCRYGG 4 cut(s) 342, 541, 660, 969
BstENI CCTNNNNNAGG 1 cut(s) 232
BstF5I GGATG 1 cut(s) 903
BstFNI CGCG 1 cut(s) 648
BstH2I RGCGCY 1 cut(s) 1134
BstHHI GCGC 5 cut(s) 648, 650, 976, 1046, 1133
BstMAI GTCTC 3 cut(s) 67, 813, 1128
BstMCI CGRYCG 4 cut(s) 294, 342, 351, 397
BstNI CCWGG 5 cut(s) 229, 595, 805, 857, 895
BstSCI CCNGG 9 cut(s) 227, 396, 435, 593, 803, 855, 893, 1017, 1153
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 2 cut(s) 295, 313
BstV2I GAAGAC 1 cut(s) 858
BstZI CGGCCG 3 cut(s) 291, 339, 394
BtgI CCRYGG 4 cut(s) 342, 541, 660, 969
BtsCI GGATG 1 cut(s) 903
BtsI GCAGTG 3 cut(s) 297, 528, 867
BtsIMutI CAGTG 5 cut(s) 297, 528, 573, 621, 867
BveI ACCTGC 1 cut(s) 951
CaiI CAGNNNCTG 1 cut(s) 326
CciNI GCGGCCGC 1 cut(s) 291
CfoI GCGC 5 cut(s) 648, 650, 976, 1046, 1133
Cfr10I RCCGGY 1 cut(s) 1027
Cfr13I GGNCC 5 cut(s) 237, 408, 421, 598, 795
CseI GACGC 1 cut(s) 1033
CviAII CATG 2 cut(s) 382, 1087
DdeI CTNAG 6 cut(s) 233, 333, 461, 839, 1098, 1316
EaeI YGGCCR 7 cut(s) 189, 291, 339, 394, 447, 891, 1029
EagI CGGCCG 3 cut(s) 291, 339, 394
Eam1104I CTCTTC 1 cut(s) 1160
EarI CTCTTC 1 cut(s) 1160
EciI GGCGGA 1 cut(s) 344
Ecl136II GAGCTC 1 cut(s) 467
EclXI CGGCCG 3 cut(s) 291, 339, 394
Eco24I GRGCYC 1 cut(s) 469
Eco31I GGTCTC 3 cut(s) 67, 813, 1128
Eco47I GGWCC 2 cut(s) 237, 421
Eco47III AGCGCT 1 cut(s) 1132
Eco52I CGGCCG 3 cut(s) 291, 339, 394
Eco53kI GAGCTC 1 cut(s) 467
Eco57I CTGAAG 1 cut(s) 645
Eco88I CYCGRG 1 cut(s) 1190
EcoICRI GAGCTC 1 cut(s) 467
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoO109I RGGNCCY 2 cut(s) 237, 598
EcoRII CCWGG 5 cut(s) 227, 593, 803, 855, 893
EcoT38I GRGCYC 1 cut(s) 469
FaeI CATG 2 cut(s) 385, 1090
FaiI YATR 6 cut(s) 183, 383, 442, 548, 588, 1088
FaqI GGGAC 3 cut(s) 62, 954, 1171
FatI CATG 2 cut(s) 381, 1086
FauI CCCGC 2 cut(s) 207, 998
FokI GGATG 1 cut(s) 890
FriOI GRGCYC 1 cut(s) 469
FspBI CTAG 1 cut(s) 363
GlaI GCGC 5 cut(s) 647, 649, 975, 1045, 1132
HaeII RGCGCY 1 cut(s) 1134
HgaI GACGC 1 cut(s) 1033
HhaI GCGC 5 cut(s) 648, 650, 976, 1046, 1133
Hin1I GRCGYC 1 cut(s) 1025
Hin1II CATG 2 cut(s) 385, 1090
Hin6I GCGC 5 cut(s) 646, 648, 974, 1044, 1131
HinP1I GCGC 5 cut(s) 646, 648, 974, 1044, 1131
HincII GTYRAC 1 cut(s) 900
HindII GTYRAC 1 cut(s) 900
HindIII AAGCTT 3 cut(s) 126, 133, 1283
HinfI GANTC 9 cut(s) 86, 92, 151, 169, 636, 679, 929, 942, 1193
HphI GGTGA 3 cut(s) 204, 493, 937
Hpy166II GTNNAC 3 cut(s) 900, 997, 1238
Hpy188III TCNNGA 1 cut(s) 1264
Hpy8I GTNNAC 3 cut(s) 900, 997, 1238
Hpy99I CGWCG 3 cut(s) 169, 848, 1027
HpyAV CCTTC 2 cut(s) 250, 786
HpyCH4III ACNGT 5 cut(s) 216, 545, 577, 743, 1205
HpyCH4V TGCA 4 cut(s) 550, 960, 1225, 1246
HpyF3I CTNAG 6 cut(s) 233, 333, 461, 839, 1098, 1316
Hsp92I GRCGYC 1 cut(s) 1025
Hsp92II CATG 2 cut(s) 385, 1090
HspAI GCGC 5 cut(s) 646, 648, 974, 1044, 1131
KroI GCCGGC 1 cut(s) 1027
KroNI GCCGGC 1 cut(s) 1029
LmnI GCTCC 5 cut(s) 684, 700, 918, 1138, 1150
Lsp1109I GCAGC 2 cut(s) 295, 313
LweI GCATC 2 cut(s) 304, 1212
MaeI CTAG 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 486
MboII GAAGA 3 cut(s) 858, 1174, 1177
MhlI GDGCHC 5 cut(s) 66, 469, 568, 983, 1097
MlsI TGGCCA 1 cut(s) 449
MluCI AATT 4 cut(s) 79, 762, 848, 1258
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 6 cut(s) 160, 163, 688, 936, 938, 1202
MmeI TCCRAC 2 cut(s) 662, 957
Mox20I TGGCCA 1 cut(s) 449
MroNI GCCGGC 1 cut(s) 1027
MscI TGGCCA 1 cut(s) 449
MseI TTAA 7 cut(s) 24, 42, 615, 1179, 1218, 1257, 1327
Msp20I TGGCCA 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 308
MspCI CTTAAG 1 cut(s) 23
MspR9I CCNGG 9 cut(s) 229, 398, 437, 595, 805, 857, 895, 1019, 1155
Mva1269I GAATGC 2 cut(s) 962, 1273
MvaI CCWGG 5 cut(s) 229, 595, 805, 857, 895
MvnI CGCG 1 cut(s) 648
NaeI GCCGGC 1 cut(s) 1029
NciI CCSGG 4 cut(s) 398, 437, 1019, 1155
NgoMIV GCCGGC 1 cut(s) 1027
NlaIII CATG 2 cut(s) 385, 1090
NlaIV GGNNCC 3 cut(s) 379, 599, 1152
NmeAIII GCCGAG 1 cut(s) 1011
NotI GCGGCCGC 1 cut(s) 291
PaeR7I CTCGAG 1 cut(s) 1190
PauI GCGCGC 1 cut(s) 646
PctI GAATGC 2 cut(s) 962, 1273
PdiI GCCGGC 1 cut(s) 1029
PfeI GAWTC 3 cut(s) 86, 92, 636
PleI GAGTC 6 cut(s) 159, 163, 687, 936, 937, 1201
PpsI GAGTC 6 cut(s) 159, 163, 687, 936, 937, 1201
PpuMI RGGWCCY 1 cut(s) 237
PshBI ATTAAT 1 cut(s) 1257
Psp124BI GAGCTC 1 cut(s) 469
Psp5II RGGWCCY 1 cut(s) 237
Psp6I CCWGG 5 cut(s) 227, 593, 803, 855, 893
PspGI CCWGG 5 cut(s) 227, 593, 803, 855, 893
PspN4I GGNNCC 3 cut(s) 379, 599, 1152
PspPI GGNCC 5 cut(s) 237, 408, 421, 598, 795
PspPPI RGGWCCY 1 cut(s) 237
PspXI VCTCGAGB 1 cut(s) 1190
PstNI CAGNNNCTG 1 cut(s) 326
PteI GCGCGC 1 cut(s) 646
SacI GAGCTC 1 cut(s) 469
SaqAI TTAA 7 cut(s) 24, 42, 615, 1179, 1218, 1257, 1327
Sau96I GGNCC 5 cut(s) 237, 408, 421, 598, 795
SchI GAGTC 6 cut(s) 160, 163, 688, 936, 938, 1202
ScrFI CCNGG 9 cut(s) 229, 398, 437, 595, 805, 857, 895, 1019, 1155
SduI GDGCHC 5 cut(s) 66, 469, 568, 983, 1097
SfaNI GCATC 2 cut(s) 304, 1212
Sfr274I CTCGAG 1 cut(s) 1190
SinI GGWCC 2 cut(s) 237, 421
SlaI CTCGAG 1 cut(s) 1190
SmlI CTYRAG 2 cut(s) 23, 1190
SmoI CTYRAG 2 cut(s) 23, 1190
Sse9I AATT 4 cut(s) 79, 762, 848, 1258
SspMI CTAG 1 cut(s) 363
SstI GAGCTC 1 cut(s) 469
StyD4I CCNGG 9 cut(s) 227, 396, 435, 593, 803, 855, 893, 1017, 1153
TaaI ACNGT 5 cut(s) 216, 545, 577, 743, 1205
TaqI TCGA 2 cut(s) 639, 1191
TasI AATT 4 cut(s) 79, 762, 848, 1258
TfiI GAWTC 3 cut(s) 86, 92, 636
Tru1I TTAA 7 cut(s) 24, 42, 615, 1179, 1218, 1257, 1327
Tru9I TTAA 7 cut(s) 24, 42, 615, 1179, 1218, 1257, 1327
TscAI CASTG 5 cut(s) 304, 535, 580, 628, 867
TseI GCWGC 2 cut(s) 308, 326
TspDTI ATGAA 3 cut(s) 264, 276, 774
TspGWI ACGGA 1 cut(s) 677
TspRI CASTG 5 cut(s) 304, 535, 580, 628, 867
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 2 cut(s) 237, 421
VspI ATTAAT 1 cut(s) 1257
XagI CCTNNNNNAGG 1 cut(s) 232
XapI RAATTY 2 cut(s) 762, 848
XhoI CTCGAG 1 cut(s) 1190
XspI CTAG 1 cut(s) 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.