FvH4_6g32490

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002379
Physical Location & Seq
Forward (+)
25543532 .. 25547356
3825 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32490.t3

Sequence Viewer

Length: 1224 bp
ATGGGGAGAGCTGCAAGATGGTTGAAGGGTCTGTTGGGGATGAAGAAAGAAAAAGACCCTCTTGAGCTTCCAAGTAGTGATACCAATACTACCCGGCCTGCTGATCGGAAAGATAAGAGAAGGTGGAGTTTCGGCAAGTCATTGAAAGATGGAACTCCAATTAGTCATGTCCCGGCTAATCTCCCGCCGAATATTTCCCCTGCTGACTCAGCCTGGCTCAGATCATATCTTGCCGAGACAGACAAGGAACAGAACAAGCACGCAATTGCGGTGGCTGCTGCCACTGCCGCCGCCGCTGACGCCGCTGTGGCTGCTGCACAGGCGGCGGTGGCGGTTGTGAGGCTGACGAGCAAGGGCTCCGGAGGACAGTTGTTTGGAAAGAGAGAGAGATGGGCGGCCATGAAGATTCAGACTGTTTTCAGAGGCTATTTGGCGAGGAAGGCACACCGAGCTCTCAGAGGACTGGTTAAGTTGCAGGCTCTTGTTAGAGGTTTTCTTGTAAGGAAACGAGCAGCTGCTACTCTTCTTGGTATGCAAGCTCTTATTAGAGCACAGACAAGTGTCCGATACCAGCGAGCACGTCGGTCTTTCAATAAAGAAAACAGGTTCCTTCCCGAAAGCAGGCCTAGGAAGTCTATTGAGAGATTTGATGAAGCAAGAAGTGAATTCTACAGCAAGAGGCTTTCTGCATCTTATGAACCTTCTCTTAATAGCTTTGATGAAAGCCCAAAGATCGTTGAAATTGACACCTTCAAAACCAGATCCAGGTCTCGTCGATTCAACACCATCTTATCTGAATGTGGCGAAGACATGCATTACACCTCTTCCCCTCTGCCATGTCCAATTCCAACTCGCATTTCTGTACCTGAGGGCCATCAGCTTCAGGATTATGAATGGTATTTCAATCCTGATGAATGCAAGTACACCACCGCCCATAGTACTCCGCGTTTAGCCAGCTCTTTTCGGTCTAATGCACCCAATACACCTGCCAAGAGTGTTTGTGGAGACAGCTTCTTCCTGCCTTGCTCAAACTGCCCAAACTATATGTCAAGCACCAAGTCATTCAAGGCCAAATCAAGGTCCCACAGTGCCCCGAAGCAAAGGCCTGAACCCCTGCCTAGGAAGAAGCTTTCCCTTGATGAAATAATGGCAGCAAGAAACAGCGTTAGTGGCATTCGAATGCATCGGCCCTACAACCAAGTCCAAGAAGTCTTGAATTATTGA

Protein Analysis

408

Amino Acids

45.69

Weight (kDa)

10.22

Isoelectric Point (pI)

61.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 310 - 390 1.9e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 994
Acc36I ACCTGC 1 cut(s) 994
AccII CGCG 1 cut(s) 946
AccIII TCCGGA 1 cut(s) 359
AclWI GGATC 1 cut(s) 756
AcoI YGGCCR 1 cut(s) 396
AcsI RAATTY 1 cut(s) 665
AcuI CTGAAG 1 cut(s) 866
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 3 cut(s) 864, 923, 940
AfiI CCNNNNNNNGG 4 cut(s) 621, 765, 1077, 1119
AgsI TTSAA 9 cut(s) 25, 145, 592, 740, 754, 781, 904, 1066, 1216
AjiI CACGTC 1 cut(s) 581
AjnI CCWGG 2 cut(s) 212, 764
AjuI GAANNNNNNNTTGG 4 cut(s) 17, 49, 841, 873
Alw21I GWGCWC 3 cut(s) 454, 553, 580
Alw26I GTCTC 3 cut(s) 230, 774, 999
AlwI GGATC 1 cut(s) 756
Aor13HI TCCGGA 1 cut(s) 359
AoxI GGCC 7 cut(s) 95, 396, 623, 871, 1068, 1103, 1187
ApeKI GCWGC 8 cut(s) 11, 275, 278, 311, 314, 512, 515, 1151
ApoI RAATTY 1 cut(s) 665
ArsI GACNNNNNNTTYG 2 cut(s) 1031, 1063
AspA2I CCTAGG 2 cut(s) 626, 1118
AspS9I GGNCC 3 cut(s) 871, 1080, 1188
AsuC2I CCSGG 2 cut(s) 94, 173
AsuII TTCGAA 1 cut(s) 1177
AvaII GGWCC 1 cut(s) 1080
AvrII CCTAGG 2 cut(s) 626, 1118
AxyI CCTNAGG 1 cut(s) 867
BaeGI GKGCMC 1 cut(s) 1093
BanII GRGCYC 2 cut(s) 359, 454
BbsI GAAGAC 1 cut(s) 813
Bbv12I GWGCWC 3 cut(s) 454, 553, 580
BbvI GCAGC 7 cut(s) 262, 265, 298, 301, 502, 524, 1163
BccI CCATC 5 cut(s) 12, 143, 384, 794, 882
BciT130I CCWGG 2 cut(s) 214, 766
BcnI CCSGG 2 cut(s) 94, 173
BcoDI GTCTC 3 cut(s) 230, 774, 999
BfaI CTAG 2 cut(s) 627, 1119
BfmI CTRYAG 1 cut(s) 670
BfuAI ACCTGC 1 cut(s) 994
BglI GCCNNNNNGGC 1 cut(s) 308
BlnI CCTAGG 2 cut(s) 626, 1118
BmcAI AGTACT 1 cut(s) 940
Bme1390I CCNGG 4 cut(s) 94, 173, 214, 766
Bme18I GGWCC 1 cut(s) 1080
BmgBI CACGTC 1 cut(s) 581
BmgT120I GGNCC 3 cut(s) 871, 1080, 1188
BmiI GGNNCC 3 cut(s) 358, 608, 1082
BmrFI CCNGG 4 cut(s) 94, 173, 214, 766
BmsI GCATC 2 cut(s) 698, 1192
BoxI GACNNNNGTC 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 813
Bpu14I TTCGAA 1 cut(s) 1177
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 2 cut(s) 94, 173
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 774
BsaJI CCNNGG 2 cut(s) 626, 1118
BsaWI WCCGGW 1 cut(s) 359
Bsc4I CCNNNNNNNGG 4 cut(s) 621, 765, 1077, 1119
Bse1I ACTGG 1 cut(s) 468
Bse21I CCTNAGG 1 cut(s) 867
BseAI TCCGGA 1 cut(s) 359
BseBI CCWGG 2 cut(s) 214, 766
BseDI CCNNGG 2 cut(s) 626, 1118
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 4 cut(s) 621, 765, 1077, 1119
BseMII CTCAG 4 cut(s) 222, 232, 469, 858
BseNI ACTGG 1 cut(s) 468
BseSI GKGCMC 1 cut(s) 1093
BseXI GCAGC 7 cut(s) 262, 265, 298, 301, 502, 524, 1163
BsgI GTGCAG 1 cut(s) 300
Bsh1236I CGCG 1 cut(s) 946
BshFI GGCC 7 cut(s) 97, 398, 625, 873, 1070, 1105, 1189
BsiHKAI GWGCWC 3 cut(s) 454, 553, 580
BsiSI CCGG 3 cut(s) 94, 173, 360
BslFI GGGAC 2 cut(s) 155, 1066
BslI CCNNNNNNNGG 4 cut(s) 621, 765, 1077, 1119
BsmAI GTCTC 3 cut(s) 230, 774, 999
BsmFI GGGAC 2 cut(s) 155, 1066
BsmI GAATGC 3 cut(s) 920, 1173, 1185
BsnI GGCC 7 cut(s) 97, 398, 625, 873, 1070, 1105, 1189
Bso31I GGTCTC 1 cut(s) 774
Bsp119I TTCGAA 1 cut(s) 1177
Bsp1286I GDGCHC 5 cut(s) 359, 454, 553, 580, 1093
Bsp13I TCCGGA 1 cut(s) 359
Bsp143I GATC 4 cut(s) 103, 221, 732, 761
BspANI GGCC 7 cut(s) 97, 398, 625, 873, 1070, 1105, 1189
BspCNI CTCAG 4 cut(s) 221, 231, 468, 859
BspEI TCCGGA 1 cut(s) 359
BspFNI CGCG 1 cut(s) 946
BspLI GGNNCC 3 cut(s) 358, 608, 1082
BspMI ACCTGC 1 cut(s) 994
BspPI GGATC 1 cut(s) 756
BspT104I TTCGAA 1 cut(s) 1177
BspTNI GGTCTC 1 cut(s) 774
BsrI ACTGG 1 cut(s) 468
BssECI CCNNGG 2 cut(s) 626, 1118
BssMI GATC 4 cut(s) 103, 221, 732, 761
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 626, 1118
Bst2UI CCWGG 2 cut(s) 214, 766
Bst4CI ACNGT 3 cut(s) 369, 415, 1088
Bst6I CTCTTC 2 cut(s) 528, 829
BstACI GRCGYC 1 cut(s) 300
BstBI TTCGAA 1 cut(s) 1177
BstC8I GCNNGC 7 cut(s) 99, 261, 477, 537, 576, 623, 955
BstDEI CTNAG 4 cut(s) 208, 218, 455, 867
BstF5I GGATG 1 cut(s) 45
BstFNI CGCG 1 cut(s) 946
BstKTI GATC 4 cut(s) 106, 224, 735, 764
BstMAI GTCTC 3 cut(s) 230, 774, 999
BstMBI GATC 4 cut(s) 103, 221, 732, 761
BstNI CCWGG 2 cut(s) 214, 766
BstNSI RCATGY 1 cut(s) 814
BstPAI GACNNNNGTC 1 cut(s) 560
BstSCI CCNGG 4 cut(s) 92, 171, 212, 764
BstSFI CTRYAG 1 cut(s) 670
BstSLI GKGCMC 1 cut(s) 1093
BstUI CGCG 1 cut(s) 946
BstV1I GCAGC 7 cut(s) 262, 265, 298, 301, 502, 524, 1163
BstV2I GAAGAC 1 cut(s) 813
BstX2I RGATCY 1 cut(s) 761
BstYI RGATCY 1 cut(s) 761
Bsu36I CCTNAGG 1 cut(s) 867
BsuRI GGCC 7 cut(s) 97, 398, 625, 873, 1070, 1105, 1189
BtrI CACGTC 1 cut(s) 581
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 2 cut(s) 282, 1093
BveI ACCTGC 1 cut(s) 994
Cac8I GCNNGC 7 cut(s) 99, 261, 477, 537, 576, 623, 955
Cfr13I GGNCC 3 cut(s) 871, 1080, 1188
CseI GACGC 1 cut(s) 308
Csp6I GTAC 3 cut(s) 863, 922, 939
CspCI CAANNNNNGTGG 2 cut(s) 252, 287
CviAII CATG 4 cut(s) 167, 400, 811, 837
CviQI GTAC 3 cut(s) 863, 922, 939
DdeI CTNAG 4 cut(s) 208, 218, 455, 867
DpnI GATC 4 cut(s) 105, 223, 734, 763
DpnII GATC 4 cut(s) 103, 221, 732, 761
EaeI YGGCCR 1 cut(s) 396
Eam1104I CTCTTC 2 cut(s) 528, 829
EarI CTCTTC 2 cut(s) 528, 829
Ecl136II GAGCTC 1 cut(s) 452
Eco130I CCWWGG 2 cut(s) 626, 1118
Eco147I AGGCCT 2 cut(s) 625, 1105
Eco24I GRGCYC 2 cut(s) 359, 454
Eco31I GGTCTC 1 cut(s) 774
Eco47I GGWCC 1 cut(s) 1080
Eco53kI GAGCTC 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 866
Eco81I CCTNAGG 1 cut(s) 867
EcoICRI GAGCTC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 1080
EcoRI GAATTC 1 cut(s) 665
EcoRII CCWGG 2 cut(s) 212, 764
EcoT14I CCWWGG 2 cut(s) 626, 1118
EcoT22I ATGCAT 2 cut(s) 816, 1185
EcoT38I GRGCYC 2 cut(s) 359, 454
ErhI CCWWGG 2 cut(s) 626, 1118
FaeI CATG 4 cut(s) 170, 403, 814, 840
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 155, 1066
FatI CATG 4 cut(s) 166, 399, 810, 836
FauI CCCGC 1 cut(s) 192
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 2 cut(s) 359, 454
FspBI CTAG 2 cut(s) 627, 1119
HaeIII GGCC 7 cut(s) 97, 398, 625, 873, 1070, 1105, 1189
HapII CCGG 3 cut(s) 94, 173, 360
HgaI GACGC 1 cut(s) 308
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 4 cut(s) 170, 403, 814, 840
HindIII AAGCTT 1 cut(s) 1127
HinfI GANTC 3 cut(s) 206, 406, 777
HpaII CCGG 3 cut(s) 94, 173, 360
Hpy166II GTNNAC 1 cut(s) 924
Hpy188I TCNGA 7 cut(s) 108, 221, 411, 422, 458, 566, 796
Hpy188III TCNNGA 6 cut(s) 62, 360, 614, 884, 908, 1213
Hpy8I GTNNAC 1 cut(s) 924
Hpy99I CGWCG 2 cut(s) 585, 777
HpyAV CCTTC 6 cut(s) 19, 114, 433, 620, 711, 760
HpyCH4III ACNGT 3 cut(s) 369, 415, 1088
HpyCH4IV ACGT 1 cut(s) 580
HpyCH4V TGCA 9 cut(s) 14, 317, 475, 535, 689, 814, 918, 974, 1183
HpyF3I CTNAG 4 cut(s) 208, 218, 455, 867
HpySE526I ACGT 1 cut(s) 580
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 4 cut(s) 170, 403, 814, 840
Kpn2I TCCGGA 1 cut(s) 359
Kzo9I GATC 4 cut(s) 103, 221, 732, 761
LmnI GCTCC 1 cut(s) 362
Lsp1109I GCAGC 7 cut(s) 262, 265, 298, 301, 502, 524, 1163
LweI GCATC 2 cut(s) 698, 1192
MaeI CTAG 2 cut(s) 627, 1119
MaeII ACGT 1 cut(s) 580
MalI GATC 4 cut(s) 105, 223, 734, 763
MboI GATC 4 cut(s) 103, 221, 732, 761
MboII GAAGA 7 cut(s) 55, 415, 515, 816, 818, 1006, 1135
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 761
MhlI GDGCHC 5 cut(s) 359, 454, 553, 580, 1093
MluCI AATT 6 cut(s) 159, 264, 665, 741, 843, 1216
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 872
Mph1103I ATGCAT 2 cut(s) 816, 1185
MroI TCCGGA 1 cut(s) 359
MseI TTAA 2 cut(s) 468, 708
MslI CAYNNNNRTG 1 cut(s) 1178
MspA1I CMGCKG 3 cut(s) 296, 305, 515
MspI CCGG 3 cut(s) 94, 173, 360
MspR9I CCNGG 4 cut(s) 94, 173, 214, 766
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 3 cut(s) 920, 1173, 1185
MvaI CCWGG 2 cut(s) 214, 766
MvnI CGCG 1 cut(s) 946
NciI CCSGG 2 cut(s) 94, 173
NdeII GATC 4 cut(s) 103, 221, 732, 761
NlaIII CATG 4 cut(s) 170, 403, 814, 840
NlaIV GGNNCC 3 cut(s) 358, 608, 1082
NmeAIII GCCGAG 1 cut(s) 259
NsiI ATGCAT 2 cut(s) 816, 1185
NspI RCATGY 1 cut(s) 814
NspV TTCGAA 1 cut(s) 1177
PaqCI CACCTGC 1 cut(s) 994
PceI AGGCCT 2 cut(s) 625, 1105
PctI GAATGC 3 cut(s) 920, 1173, 1185
PfeI GAWTC 2 cut(s) 406, 777
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 1080
PshAI GACNNNNGTC 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 1080
Psp6I CCWGG 2 cut(s) 212, 764
PspGI CCWGG 2 cut(s) 212, 764
PspN4I GGNNCC 3 cut(s) 358, 608, 1082
PspPI GGNCC 3 cut(s) 871, 1080, 1188
PspPPI RGGWCCY 1 cut(s) 1080
PsuI RGATCY 1 cut(s) 761
PvuII CAGCTG 1 cut(s) 515
RsaI GTAC 3 cut(s) 864, 923, 940
RsaNI GTAC 3 cut(s) 863, 922, 939
RseI CAYNNNNRTG 1 cut(s) 1178
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 2 cut(s) 468, 708
Sau3AI GATC 4 cut(s) 103, 221, 732, 761
Sau96I GGNCC 3 cut(s) 871, 1080, 1188
ScaI AGTACT 1 cut(s) 940
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 4 cut(s) 94, 173, 214, 766
SduI GDGCHC 5 cut(s) 359, 454, 553, 580, 1093
SfaNI GCATC 2 cut(s) 698, 1192
SfcI CTRYAG 1 cut(s) 670
SfuI TTCGAA 1 cut(s) 1177
SinI GGWCC 1 cut(s) 1080
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 6 cut(s) 159, 264, 665, 741, 843, 1216
SseBI AGGCCT 2 cut(s) 625, 1105
SspI AATATT 1 cut(s) 193
SspMI CTAG 2 cut(s) 627, 1119
SstI GAGCTC 1 cut(s) 454
StuI AGGCCT 2 cut(s) 625, 1105
StyD4I CCNGG 4 cut(s) 92, 171, 212, 764
StyI CCWWGG 2 cut(s) 626, 1118
TaaI ACNGT 3 cut(s) 369, 415, 1088
TaiI ACGT 1 cut(s) 583
TaqI TCGA 2 cut(s) 775, 1177
TaqII GACCGA 2 cut(s) 573, 954
TasI AATT 6 cut(s) 159, 264, 665, 741, 843, 1216
TatI WGTACW 2 cut(s) 921, 938
TauI GCSGC 6 cut(s) 290, 293, 296, 305, 326, 398
TfiI GAWTC 2 cut(s) 406, 777
Tru1I TTAA 2 cut(s) 468, 708
Tru9I TTAA 2 cut(s) 468, 708
TscAI CASTG 2 cut(s) 289, 1093
TseI GCWGC 8 cut(s) 11, 275, 278, 311, 314, 512, 515, 1151
TspDTI ATGAA 8 cut(s) 56, 416, 666, 711, 735, 906, 927, 1155
TspRI CASTG 2 cut(s) 289, 1093
VpaK11BI GGWCC 1 cut(s) 1080
XapI RAATTY 1 cut(s) 665
XceI RCATGY 1 cut(s) 814
XmaJI CCTAGG 2 cut(s) 626, 1118
XspI CTAG 2 cut(s) 627, 1119
ZrmI AGTACT 1 cut(s) 940
Zsp2I ATGCAT 2 cut(s) 816, 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.