MD17G1182000.v1.1

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002379
Physical Location & Seq
Reverse (-)
21272381 .. 21274937
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1182000.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGGGGAGAGCTACAAGATGGTTGAAGAGCTTGCTGGGGATGAAGAGAGAGAAAGAGCACGTCGACAACACCAGCACTGTCTCCAGCGACCGGAAGGAGAAGAAAAGGTGGAGCTTCGCCAAGTCAGGGAAAGACACAACAACTAATCTTCCGCCCAATGTTCCTGTCCCTGCTGACTCTGCCTGGCTCAGATCCTACCTTGCCGACTCAGAGAAGGAGCAGAGCAAGCACGCAATTGCGGTGGCTGCGGCCACCGCAGCTGCTGCTGATGCTGCAGTGGCTGCAGCACAGGCGGCCGTCGCGGTTGTGAGGTTGACAAGTCAAGGCGGTGGCCGCGGCGGTGGTGGCGGGATCATGTTTGGGAAAAGAGAGAGATGGGCTGCAATGAAGATTCAAACTGTTTTCAGAGGCTATTTGGCGAGAAAGGCTCACCGTGCTCTTAAAGGACTGGTTAAGTTGCAGGCTCTTGTTAGAGGCTTTCTTGTCCGGAAACGGGCTGCTGCCACTCTTTACAGTATGCAAGCTCTTTTTAGAGCACAGACTACTGTCCGATACCAGCGTGCGCGTCGTTCTTTCAATAAAGAGAATAGGTTCCTACCCGAAATTAATGCCCGGAAGTCTGTTGAAAGATTTGAAGATGCAAGAAGTGAATTTTATAGCAGGAGGCTTTCTGCAACTTATGAAACTTCGGTTAATAGCTTTGATGAAAGCCCGAAGATTGTGGAAATTGACACATTCAAGCCGAGATCCAGGTCTCATAGGTTCAACACCATATTATCTGATTGTGGCGAAGACCTGCCTTACCAAACCATCTCCTCACCGCTGCCCTGTCCCATGCCAGCTCGTATCTCTATACCTGACAGCAAGAAACCTCAAGATTTTGAATGGTACTTCAATGGTAATGAATGCAAGTTCCCGACTGCCCATAACACTCCGCGGTTTGCCACCTCTTTTCGTTCTAATGCTCCCGGCACACCATCCAAAAGTGTTTGTGGAGACAGCTTCTTTCGGCCTTACTCAGACTCCCCAAACTACATGTCAAGCACTCAGTCATTTAATGCCAAGTCGAGATCCCACAGCGCCCCAAAGCAAAGGCCGGAACCAGGGCCTAAGAAGAGGCTTTCGCTTAATGAAATGATGGCAGCGAGAAACAGCATCAGCGGCATTCGAATGCATCGATCGTGTAATCAAATCCAAGAAGAAGTCTCGAATGATTGA

Protein Analysis

406

Amino Acids

45.26

Weight (kDa)

10.26

Isoelectric Point (pI)

59.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 312 - 387 4.1e-10 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 804
AccI GTMKAC 1 cut(s) 63
AccII CGCG 4 cut(s) 302, 336, 565, 937
AccIII TCCGGA 1 cut(s) 486
AclWI GGATC 4 cut(s) 186, 359, 741, 1065
AcoI YGGCCR 3 cut(s) 249, 294, 331
AcsI RAATTY 1 cut(s) 650
AfaI GTAC 1 cut(s) 890
AfiI CCNNNNNNNGG 4 cut(s) 90, 126, 493, 1103
AflIII ACRYGT 1 cut(s) 1035
AgsI TTSAA 9 cut(s) 25, 395, 577, 626, 635, 739, 766, 884, 895
AjiI CACGTC 1 cut(s) 61
AjnI CCWGG 3 cut(s) 182, 749, 1102
AluBI AGCT 8 cut(s) 11, 30, 114, 260, 524, 699, 842, 1002
AluI AGCT 8 cut(s) 11, 30, 114, 260, 524, 699, 842, 1002
Alw21I GWGCWC 3 cut(s) 60, 439, 538
Alw26I GTCTC 4 cut(s) 85, 759, 990, 1210
AlwI GGATC 4 cut(s) 186, 359, 741, 1065
AlwNI CAGNNNCTG 2 cut(s) 263, 281
Aor13HI TCCGGA 1 cut(s) 486
AoxI GGCC 6 cut(s) 249, 294, 331, 1010, 1094, 1106
ApoI RAATTY 1 cut(s) 650
ArsI GACNNNNNNTTYG 2 cut(s) 1022, 1054
AseI ATTAAT 1 cut(s) 606
Asp700I GAANNNNTTC 1 cut(s) 590
AspLEI GCGC 2 cut(s) 565, 1082
AspS9I GGNCC 1 cut(s) 1106
AsuC2I CCSGG 2 cut(s) 613, 969
AsuHPI GGTGA 2 cut(s) 422, 810
AsuII TTCGAA 1 cut(s) 1168
BaeI ACNNNNGTAYC 2 cut(s) 544, 577
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 3 cut(s) 60, 439, 538
BccI CCATC 5 cut(s) 12, 369, 818, 985, 1132
BceAI ACGGC 1 cut(s) 281
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 3 cut(s) 184, 751, 1104
BcnI CCSGG 2 cut(s) 613, 969
BcoDI GTCTC 4 cut(s) 85, 759, 990, 1210
BfmI CTRYAG 2 cut(s) 273, 282
BfoI RGCGCY 1 cut(s) 1083
BfuAI ACCTGC 1 cut(s) 804
Bme1390I CCNGG 5 cut(s) 184, 613, 751, 969, 1104
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 1106
BmiI GGNNCC 2 cut(s) 593, 1101
BmrFI CCNGG 5 cut(s) 184, 613, 751, 969, 1104
BmsI GCATC 4 cut(s) 259, 628, 1164, 1183
BoxI GACNNNNGTC 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 798
BplI GAGNNNNNCTC 2 cut(s) 412, 444
BpmI CTGGAG 1 cut(s) 67
Bpu14I TTCGAA 1 cut(s) 1168
BpuEI CTTGAG 1 cut(s) 858
BpuMI CCSGG 2 cut(s) 613, 969
Bsa29I ATCGAT 1 cut(s) 1177
BsaI GGTCTC 1 cut(s) 759
BsaJI CCNNGG 3 cut(s) 334, 935, 1103
BsaWI WCCGGW 2 cut(s) 90, 486
BsaXI ACNNNNNCTCC 2 cut(s) 1007, 1037
Bsc4I CCNNNNNNNGG 4 cut(s) 90, 126, 493, 1103
Bse1I ACTGG 1 cut(s) 453
Bse3DI GCAATG 1 cut(s) 390
BseAI TCCGGA 1 cut(s) 486
BseBI CCWGG 3 cut(s) 184, 751, 1104
BseCI ATCGAT 1 cut(s) 1177
BseDI CCNNGG 3 cut(s) 334, 935, 1103
BseGI GGATG 2 cut(s) 45, 977
BseLI CCNNNNNNNGG 4 cut(s) 90, 126, 493, 1103
BseMI GCAATG 1 cut(s) 390
BseMII CTCAG 4 cut(s) 202, 222, 1032, 1061
BseNI ACTGG 1 cut(s) 453
BseRI GAGGAG 1 cut(s) 805
BseX3I CGGCCG 1 cut(s) 294
BseYI CCCAGC 1 cut(s) 34
Bsh1236I CGCG 4 cut(s) 302, 336, 565, 937
Bsh1285I CGRYCG 3 cut(s) 91, 297, 1181
BshFI GGCC 6 cut(s) 251, 296, 333, 1012, 1096, 1108
BshVI ATCGAT 1 cut(s) 1177
BsiEI CGRYCG 3 cut(s) 91, 297, 1181
BsiHKAI GWGCWC 3 cut(s) 60, 439, 538
BsiSI CCGG 5 cut(s) 91, 487, 613, 969, 1097
BslFI GGGAC 2 cut(s) 152, 816
BslI CCNNNNNNNGG 4 cut(s) 90, 126, 493, 1103
BsmAI GTCTC 4 cut(s) 85, 759, 990, 1210
BsmFI GGGAC 2 cut(s) 152, 816
BsmI GAATGC 3 cut(s) 911, 1164, 1176
BsnI GGCC 6 cut(s) 251, 296, 333, 1012, 1096, 1108
Bso31I GGTCTC 1 cut(s) 759
Bsp119I TTCGAA 1 cut(s) 1168
Bsp1286I GDGCHC 3 cut(s) 60, 439, 538
Bsp13I TCCGGA 1 cut(s) 486
Bsp143I GATC 5 cut(s) 191, 351, 746, 1070, 1178
BspANI GGCC 6 cut(s) 251, 296, 333, 1012, 1096, 1108
BspCNI CTCAG 4 cut(s) 201, 221, 1031, 1060
BspDI ATCGAT 1 cut(s) 1177
BspEI TCCGGA 1 cut(s) 486
BspFNI CGCG 4 cut(s) 302, 336, 565, 937
BspLI GGNNCC 2 cut(s) 593, 1101
BspMAI CTGCAG 2 cut(s) 277, 286
BspMI ACCTGC 1 cut(s) 804
BspPI GGATC 4 cut(s) 186, 359, 741, 1065
BspQI GCTCTTC 1 cut(s) 20
BspT104I TTCGAA 1 cut(s) 1168
BspTNI GGTCTC 1 cut(s) 759
BsrDI GCAATG 1 cut(s) 390
BsrI ACTGG 1 cut(s) 453
BssECI CCNNGG 3 cut(s) 334, 935, 1103
BssMI GATC 5 cut(s) 191, 351, 746, 1070, 1178
Bst2UI CCWGG 3 cut(s) 184, 751, 1104
Bst4CI ACNGT 5 cut(s) 79, 400, 434, 515, 547
Bst6I CTCTTC 3 cut(s) 20, 38, 1109
BstAPI GCANNNNNTGC 2 cut(s) 263, 281
BstBI TTCGAA 1 cut(s) 1168
BstC8I GCNNGC 7 cut(s) 32, 227, 231, 462, 522, 561, 840
BstDEI CTNAG 5 cut(s) 188, 208, 1018, 1047, 1110
BstDSI CCRYGG 2 cut(s) 334, 935
BstF5I GGATG 2 cut(s) 45, 977
BstFNI CGCG 4 cut(s) 302, 336, 565, 937
BstH2I RGCGCY 1 cut(s) 1083
BstHHI GCGC 2 cut(s) 565, 1082
BstKTI GATC 5 cut(s) 194, 354, 749, 1073, 1181
BstMAI GTCTC 4 cut(s) 85, 759, 990, 1210
BstMBI GATC 5 cut(s) 191, 351, 746, 1070, 1178
BstMCI CGRYCG 3 cut(s) 91, 297, 1181
BstNI CCWGG 3 cut(s) 184, 751, 1104
BstNSI RCATGY 1 cut(s) 1039
BstPAI GACNNNNGTC 1 cut(s) 545
BstSCI CCNGG 5 cut(s) 182, 611, 749, 967, 1102
BstSFI CTRYAG 2 cut(s) 273, 282
BstUI CGCG 4 cut(s) 302, 336, 565, 937
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 3 cut(s) 191, 746, 1070
BstYI RGATCY 3 cut(s) 191, 746, 1070
BstZI CGGCCG 1 cut(s) 294
Bsu15I ATCGAT 1 cut(s) 1177
BsuRI GGCC 6 cut(s) 251, 296, 333, 1012, 1096, 1108
BsuTUI ATCGAT 1 cut(s) 1177
BtgI CCRYGG 2 cut(s) 334, 935
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 45, 977
BtsI GCAGTG 1 cut(s) 282
BtsIMutI CAGTG 2 cut(s) 75, 282
BveI ACCTGC 1 cut(s) 804
Cac8I GCNNGC 7 cut(s) 32, 227, 231, 462, 522, 561, 840
CaiI CAGNNNCTG 2 cut(s) 263, 281
CfoI GCGC 2 cut(s) 565, 1082
Cfr13I GGNCC 1 cut(s) 1106
Cfr42I CCGCGG 2 cut(s) 337, 938
ClaI ATCGAT 1 cut(s) 1177
CseI GACGC 1 cut(s) 554
Csp6I GTAC 1 cut(s) 889
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviAII CATG 3 cut(s) 355, 835, 1036
CviQI GTAC 1 cut(s) 889
DdeI CTNAG 5 cut(s) 188, 208, 1018, 1047, 1110
DpnI GATC 5 cut(s) 193, 353, 748, 1072, 1180
DpnII GATC 5 cut(s) 191, 351, 746, 1070, 1178
EaeI YGGCCR 3 cut(s) 249, 294, 331
EagI CGGCCG 1 cut(s) 294
Eam1104I CTCTTC 3 cut(s) 20, 38, 1109
EarI CTCTTC 3 cut(s) 20, 38, 1109
EciI GGCGGA 1 cut(s) 141
EclXI CGGCCG 1 cut(s) 294
Eco31I GGTCTC 1 cut(s) 759
Eco52I CGGCCG 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 1106
EcoRII CCWGG 3 cut(s) 182, 749, 1102
EcoT22I ATGCAT 1 cut(s) 1176
FaeI CATG 3 cut(s) 358, 838, 1039
FaqI GGGAC 2 cut(s) 152, 816
FatI CATG 3 cut(s) 354, 834, 1035
FauI CCCGC 1 cut(s) 341
FblI GTMKAC 1 cut(s) 63
FokI GGATG 2 cut(s) 52, 964
GlaI GCGC 2 cut(s) 564, 1081
GsaI CCCAGC 1 cut(s) 38
GsuI CTGGAG 1 cut(s) 67
HaeII RGCGCY 1 cut(s) 1083
HaeIII GGCC 6 cut(s) 251, 296, 333, 1012, 1096, 1108
HapII CCGG 5 cut(s) 91, 487, 613, 969, 1097
HgaI GACGC 1 cut(s) 554
HhaI GCGC 2 cut(s) 565, 1082
Hin1II CATG 3 cut(s) 358, 838, 1039
Hin6I GCGC 2 cut(s) 563, 1080
HinP1I GCGC 2 cut(s) 563, 1080
HincII GTYRAC 2 cut(s) 64, 315
HindII GTYRAC 2 cut(s) 64, 315
HinfI GANTC 4 cut(s) 176, 206, 391, 1022
HpaII CCGG 5 cut(s) 91, 487, 613, 969, 1097
HphI GGTGA 2 cut(s) 422, 810
Hpy166II GTNNAC 2 cut(s) 64, 315
Hpy188I TCNGA 6 cut(s) 191, 211, 407, 551, 781, 1021
Hpy188III TCNNGA 5 cut(s) 487, 875, 916, 1068, 1207
Hpy8I GTNNAC 2 cut(s) 64, 315
Hpy99I CGWCG 3 cut(s) 65, 302, 570
HpyAV CCTTC 2 cut(s) 88, 208
HpyCH4III ACNGT 5 cut(s) 79, 400, 434, 515, 547
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 9 cut(s) 275, 284, 383, 460, 520, 641, 674, 909, 1174
HpyF3I CTNAG 5 cut(s) 188, 208, 1018, 1047, 1110
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 3 cut(s) 358, 838, 1039
HspAI GCGC 2 cut(s) 563, 1080
Kpn2I TCCGGA 1 cut(s) 486
KspI CCGCGG 2 cut(s) 337, 938
Kzo9I GATC 5 cut(s) 191, 351, 746, 1070, 1178
LguI GCTCTTC 1 cut(s) 20
LmnI GCTCC 3 cut(s) 111, 217, 970
LweI GCATC 4 cut(s) 259, 628, 1164, 1183
MaeII ACGT 1 cut(s) 60
MalI GATC 5 cut(s) 193, 353, 748, 1072, 1180
MboI GATC 5 cut(s) 191, 351, 746, 1070, 1178
MfeI CAATTG 1 cut(s) 234
MflI RGATCY 3 cut(s) 191, 746, 1070
MhlI GDGCHC 3 cut(s) 60, 439, 538
MluCI AATT 4 cut(s) 234, 603, 650, 726
MlyI GAGTC 3 cut(s) 170, 200, 1016
MnlI CCTC 8 cut(s) 303, 401, 467, 657, 826, 882, 958, 1110
Mph1103I ATGCAT 1 cut(s) 1176
MroI TCCGGA 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 590
MseI TTAA 6 cut(s) 441, 453, 606, 693, 1056, 1128
MslI CAYNNNNRTG 1 cut(s) 1169
MspA1I CMGCKG 5 cut(s) 260, 336, 823, 937, 1161
MspI CCGG 5 cut(s) 91, 487, 613, 969, 1097
MspR9I CCNGG 5 cut(s) 184, 613, 751, 969, 1104
MunI CAATTG 1 cut(s) 234
Mva1269I GAATGC 3 cut(s) 911, 1164, 1176
MvaI CCWGG 3 cut(s) 184, 751, 1104
MvnI CGCG 4 cut(s) 302, 336, 565, 937
NciI CCSGG 2 cut(s) 613, 969
NdeII GATC 5 cut(s) 191, 351, 746, 1070, 1178
NlaIII CATG 3 cut(s) 358, 838, 1039
NlaIV GGNNCC 2 cut(s) 593, 1101
NmeAIII GCCGAG 1 cut(s) 768
NsiI ATGCAT 1 cut(s) 1176
NspI RCATGY 1 cut(s) 1039
NspV TTCGAA 1 cut(s) 1168
PciI ACATGT 1 cut(s) 1035
PciSI GCTCTTC 1 cut(s) 20
PcsI WCGNNNNNNNCGW 1 cut(s) 1174
PctI GAATGC 3 cut(s) 911, 1164, 1176
PdmI GAANNNNTTC 1 cut(s) 590
PfeI GAWTC 1 cut(s) 391
Ple19I CGATCG 1 cut(s) 1181
PleI GAGTC 3 cut(s) 170, 200, 1016
PpsI GAGTC 3 cut(s) 170, 200, 1016
PscI ACATGT 1 cut(s) 1035
PshAI GACNNNNGTC 1 cut(s) 545
PshBI ATTAAT 1 cut(s) 606
Psp6I CCWGG 3 cut(s) 182, 749, 1102
PspFI CCCAGC 1 cut(s) 34
PspGI CCWGG 3 cut(s) 182, 749, 1102
PspN4I GGNNCC 2 cut(s) 593, 1101
PspPI GGNCC 1 cut(s) 1106
PstI CTGCAG 2 cut(s) 277, 286
PstNI CAGNNNCTG 2 cut(s) 263, 281
PsuI RGATCY 3 cut(s) 191, 746, 1070
PvuI CGATCG 1 cut(s) 1181
PvuII CAGCTG 1 cut(s) 260
RsaI GTAC 1 cut(s) 890
RsaNI GTAC 1 cut(s) 889
RseI CAYNNNNRTG 1 cut(s) 1169
SacII CCGCGG 2 cut(s) 337, 938
SalI GTCGAC 1 cut(s) 62
SapI GCTCTTC 1 cut(s) 20
SaqAI TTAA 6 cut(s) 441, 453, 606, 693, 1056, 1128
Sau3AI GATC 5 cut(s) 191, 351, 746, 1070, 1178
Sau96I GGNCC 1 cut(s) 1106
SchI GAGTC 3 cut(s) 170, 200, 1016
ScrFI CCNGG 5 cut(s) 184, 613, 751, 969, 1104
SduI GDGCHC 3 cut(s) 60, 439, 538
SfaNI GCATC 4 cut(s) 259, 628, 1164, 1183
SfcI CTRYAG 2 cut(s) 273, 282
Sfr303I CCGCGG 2 cut(s) 337, 938
SfuI TTCGAA 1 cut(s) 1168
SgrBI CCGCGG 2 cut(s) 337, 938
SmiMI CAYNNNNRTG 1 cut(s) 1169
SmlI CTYRAG 1 cut(s) 873
SmoI CTYRAG 1 cut(s) 873
Sse9I AATT 4 cut(s) 234, 603, 650, 726
StyD4I CCNGG 5 cut(s) 182, 611, 749, 967, 1102
TaaI ACNGT 5 cut(s) 79, 400, 434, 515, 547
TaiI ACGT 1 cut(s) 63
TaqI TCGA 5 cut(s) 63, 1067, 1168, 1177, 1208
TasI AATT 4 cut(s) 234, 603, 650, 726
TauI GCSGC 5 cut(s) 251, 296, 336, 339, 1164
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 6 cut(s) 441, 453, 606, 693, 1056, 1128
Tru9I TTAA 6 cut(s) 441, 453, 606, 693, 1056, 1128
TscAI CASTG 2 cut(s) 82, 282
TspDTI ATGAA 6 cut(s) 56, 401, 696, 720, 918, 1146
TspRI CASTG 2 cut(s) 82, 282
VspI ATTAAT 1 cut(s) 606
XapI RAATTY 1 cut(s) 650
XceI RCATGY 1 cut(s) 1039
XmiI GTMKAC 1 cut(s) 63
XmnI GAANNNNTTC 1 cut(s) 590
Zsp2I ATGCAT 1 cut(s) 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.