Rroxscaffold_2G00104500

iq-domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002379
Physical Location & Seq
Reverse (-)
27656836 .. 27660582
3747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00104500.1

Sequence Viewer

Length: 1227 bp
ATGGGGAGAGCTACAAGATGGTTGAAGGCTCTGTTGGGGATGAAGAAAGAGAAAGACCCTCTTGAGCTTCCAAGTAGTACTACCAATACTACTCGGCCTGGTGATCGGAAAGAGAAGAGAAGGTGGAGTTTCGGCAAGTCATTGAAAGATGCAAGTCCAATTAGTCATGTCCCGGCTAATCTTCCCCCGAATGTTTCCCCTGCTGACTCAGCCTGGCTCAGATCCTATCTTGCCGAGACAGAGAAGGAACAGAACAAGCACGCAATTGCGGTGGCTGCTGCCACTGCGGCCGCTGCTGACGCTGCCGTGGCTGCTGCTCAGGCGGCGGTGGCGGTTGTGAGGCTGACGAGCAAGGGCTGTGGAGGAGACTTGTTTGGTAAGAGAGAGAGATGGGCGGCAATGAGGATTCAGACTGTTTTCAGAGGCTATTTGGCCAGGAAGGCGCACCGAGCTCTTAGAGGACTTGTTAAGTTGCAGGCTCTTGTTAGAGGCTTTCTTGTCAGGAAACGAGCTGCTGCTACTCTTCATGGTATGCAAGCTCTTATTAGAGCACAGACAAGTGTCCGATACCAGCGAGCACGTCGTTCTTTCAATAAAGAGAACAGGTTCCTACCCGAAAGCAGGCCTAGGAAGTCCATTGAGAGATTTGATGAAGCAAGAAGTGAATTTTATAGCAAGAGGCTTTCTGCAACTTATGAACCTTCTCTTAATGGCGTTGATGAAAGCCCAAAGATCGTTGAAATCGATACCTTCAGAACCAGATCCAGGTCTCGCCGATTCAACACTGTCTTATCTGAATATGGCGAAGACGTGCCTTACACCTCTTCGCCACTGCCATGTCCAATTCCAACTCGCATTACTGTACCTGGGGGCCAGCAGCTTCAGGATTATGAATGGTACTTCACTGCTGATGAATGCAAGTTCACTACAGCCCATAGTACCCCCCGTTTGGCCAGCTCTTTTCGGTCTAATGCACCCAGTACACCTGCCAAGAGTGTTTGTGGAGACAGCTTCTTTCAGCCTTACTCAAACTGCCCAAACTATATGTCAAGCACCAAGTCATTTAAGGCCAAATCAAGGTCCCACAGTGCACCGAAGCAGAGGCCTGAACCCCTGCCTAGGAAGAGGCTTTCCCTTGATGAAATAATGGCAGCAAGAAACAGTGTGAGTGGCATTCGAATGCATCGACCCAACAACCAAGTCCAAGAAGAAGTCCTGAATTATTGA

Protein Analysis

408

Amino Acids

45.75

Weight (kDa)

10.35

Isoelectric Point (pI)

61.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 312 - 390 2.4e-11 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 994
Acc36I ACCTGC 1 cut(s) 994
AciI CCGC 7 cut(s) 269, 287, 291, 323, 326, 332, 395
AclWI GGATC 2 cut(s) 216, 756
AcoI YGGCCR 3 cut(s) 288, 432, 951
AcsI RAATTY 1 cut(s) 665
AcuI CTGAAG 2 cut(s) 736, 866
AfaI GTAC 5 cut(s) 79, 864, 899, 940, 982
AfiI CCNNNNNNNGG 5 cut(s) 621, 765, 949, 1077, 1119
AgsI TTSAA 5 cut(s) 25, 145, 592, 740, 781
AjiI CACGTC 2 cut(s) 581, 811
AjnI CCWGG 5 cut(s) 97, 212, 434, 764, 865
AjuI GAANNNNNNNTTGG 2 cut(s) 17, 49
AluBI AGCT 8 cut(s) 11, 67, 452, 512, 539, 880, 957, 1011
AluI AGCT 8 cut(s) 11, 67, 452, 512, 539, 880, 957, 1011
Alw21I GWGCWC 4 cut(s) 454, 553, 580, 1093
Alw26I GTCTC 4 cut(s) 230, 360, 774, 999
Alw44I GTGCAC 1 cut(s) 1089
AlwI GGATC 2 cut(s) 216, 756
AoxI GGCC 8 cut(s) 95, 288, 432, 623, 871, 951, 1068, 1103
ApaLI GTGCAC 1 cut(s) 1089
ApoI RAATTY 1 cut(s) 665
ArsI GACNNNNNNTTYG 2 cut(s) 1031, 1063
Asp700I GAANNNNTTC 1 cut(s) 605
AspA2I CCTAGG 2 cut(s) 626, 1118
AspLEI GCGC 1 cut(s) 445
AspS9I GGNCC 2 cut(s) 871, 1080
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 1 cut(s) 113
AsuII TTCGAA 1 cut(s) 1177
AvaII GGWCC 1 cut(s) 1080
AvrII CCTAGG 2 cut(s) 626, 1118
BaeGI GKGCMC 1 cut(s) 1093
BalI TGGCCA 2 cut(s) 434, 953
BanII GRGCYC 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 813
Bbv12I GWGCWC 4 cut(s) 454, 553, 580, 1093
BccI CCATC 2 cut(s) 12, 384
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 5 cut(s) 99, 214, 436, 766, 867
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 4 cut(s) 230, 360, 774, 999
BfaI CTAG 2 cut(s) 627, 1119
BfmI CTRYAG 1 cut(s) 927
BfuAI ACCTGC 1 cut(s) 994
BglI GCCNNNNNGGC 2 cut(s) 287, 440
BlnI CCTAGG 2 cut(s) 626, 1118
BmcAI AGTACT 1 cut(s) 79
Bme1390I CCNGG 6 cut(s) 99, 173, 214, 436, 766, 867
Bme18I GGWCC 1 cut(s) 1080
BmgBI CACGTC 2 cut(s) 581, 811
BmgT120I GGNCC 2 cut(s) 871, 1080
BmiI GGNNCC 3 cut(s) 608, 872, 1082
BmrFI CCNGG 6 cut(s) 99, 173, 214, 436, 766, 867
BmrI ACTGGG 1 cut(s) 972
BmsI GCATC 2 cut(s) 139, 1192
BmuI ACTGGG 1 cut(s) 972
BoxI GACNNNNGTC 1 cut(s) 560
BpiI GAAGAC 1 cut(s) 813
Bpu10I CCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 1177
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 173
Bsa29I ATCGAT 1 cut(s) 744
BsaI GGTCTC 1 cut(s) 774
BsaJI CCNNGG 4 cut(s) 306, 626, 866, 1118
Bsc4I CCNNNNNNNGG 5 cut(s) 621, 765, 949, 1077, 1119
Bse1I ACTGG 1 cut(s) 978
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 5 cut(s) 99, 214, 436, 766, 867
BseCI ATCGAT 1 cut(s) 744
BseDI CCNNGG 4 cut(s) 306, 626, 866, 1118
BseGI GGATG 1 cut(s) 45
BseLI CCNNNNNNNGG 5 cut(s) 621, 765, 949, 1077, 1119
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 3 cut(s) 222, 232, 332
BseNI ACTGG 1 cut(s) 978
BseRI GAGGAG 1 cut(s) 378
BseSI GKGCMC 1 cut(s) 1093
BseX3I CGGCCG 1 cut(s) 288
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 8 cut(s) 97, 290, 434, 625, 873, 953, 1070, 1105
BshVI ATCGAT 1 cut(s) 744
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 4 cut(s) 454, 553, 580, 1093
BsiSI CCGG 1 cut(s) 173
BslFI GGGAC 2 cut(s) 155, 1066
BslI CCNNNNNNNGG 5 cut(s) 621, 765, 949, 1077, 1119
BsmAI GTCTC 4 cut(s) 230, 360, 774, 999
BsmFI GGGAC 2 cut(s) 155, 1066
BsmI GAATGC 3 cut(s) 920, 1173, 1185
BsnI GGCC 8 cut(s) 97, 290, 434, 625, 873, 953, 1070, 1105
Bso31I GGTCTC 1 cut(s) 774
Bsp119I TTCGAA 1 cut(s) 1177
Bsp1286I GDGCHC 4 cut(s) 454, 553, 580, 1093
Bsp143I GATC 4 cut(s) 103, 221, 732, 761
BspACI CCGC 7 cut(s) 269, 287, 291, 323, 326, 332, 395
BspANI GGCC 8 cut(s) 97, 290, 434, 625, 873, 953, 1070, 1105
BspCNI CTCAG 3 cut(s) 221, 231, 331
BspDI ATCGAT 1 cut(s) 744
BspLI GGNNCC 3 cut(s) 608, 872, 1082
BspMI ACCTGC 1 cut(s) 994
BspPI GGATC 2 cut(s) 216, 756
BspT104I TTCGAA 1 cut(s) 1177
BspTNI GGTCTC 1 cut(s) 774
BsrDI GCAATG 1 cut(s) 405
BsrI ACTGG 1 cut(s) 978
BssECI CCNNGG 4 cut(s) 306, 626, 866, 1118
BssMI GATC 4 cut(s) 103, 221, 732, 761
BssT1I CCWWGG 2 cut(s) 626, 1118
Bst2UI CCWGG 5 cut(s) 99, 214, 436, 766, 867
Bst4CI ACNGT 5 cut(s) 415, 787, 862, 1088, 1163
Bst6I CTCTTC 4 cut(s) 110, 528, 829, 1118
BstBI TTCGAA 1 cut(s) 1177
BstC8I GCNNGC 7 cut(s) 261, 477, 537, 576, 623, 875, 955
BstDEI CTNAG 4 cut(s) 208, 218, 318, 455
BstDSI CCRYGG 1 cut(s) 306
BstF5I GGATG 1 cut(s) 45
BstHHI GCGC 1 cut(s) 445
BstKTI GATC 4 cut(s) 106, 224, 735, 764
BstMAI GTCTC 4 cut(s) 230, 360, 774, 999
BstMBI GATC 4 cut(s) 103, 221, 732, 761
BstMCI CGRYCG 1 cut(s) 291
BstNI CCWGG 5 cut(s) 99, 214, 436, 766, 867
BstPAI GACNNNNGTC 1 cut(s) 560
BstSCI CCNGG 6 cut(s) 97, 171, 212, 434, 764, 865
BstSFI CTRYAG 1 cut(s) 927
BstSLI GKGCMC 1 cut(s) 1093
BstV2I GAAGAC 1 cut(s) 813
BstX2I RGATCY 2 cut(s) 221, 761
BstYI RGATCY 2 cut(s) 221, 761
BstZI CGGCCG 1 cut(s) 288
Bsu15I ATCGAT 1 cut(s) 744
BsuRI GGCC 8 cut(s) 97, 290, 434, 625, 873, 953, 1070, 1105
BsuTUI ATCGAT 1 cut(s) 744
BtgI CCRYGG 1 cut(s) 306
BtrI CACGTC 2 cut(s) 581, 811
BtsCI GGATG 1 cut(s) 45
BtsI GCAGTG 3 cut(s) 282, 830, 903
BtsIMutI CAGTG 6 cut(s) 282, 783, 830, 903, 1093, 1168
BveI ACCTGC 1 cut(s) 994
Cac8I GCNNGC 7 cut(s) 261, 477, 537, 576, 623, 875, 955
CciNI GCGGCCGC 1 cut(s) 288
CfoI GCGC 1 cut(s) 445
Cfr13I GGNCC 2 cut(s) 871, 1080
ClaI ATCGAT 1 cut(s) 744
CseI GACGC 1 cut(s) 308
Csp6I GTAC 5 cut(s) 78, 863, 898, 939, 981
CspCI CAANNNNNGTGG 4 cut(s) 252, 287, 340, 375
CviAII CATG 3 cut(s) 167, 527, 837
CviQI GTAC 5 cut(s) 78, 863, 898, 939, 981
DdeI CTNAG 4 cut(s) 208, 218, 318, 455
DpnI GATC 4 cut(s) 105, 223, 734, 763
DpnII GATC 4 cut(s) 103, 221, 732, 761
EaeI YGGCCR 3 cut(s) 288, 432, 951
EagI CGGCCG 1 cut(s) 288
Eam1104I CTCTTC 4 cut(s) 110, 528, 829, 1118
EarI CTCTTC 4 cut(s) 110, 528, 829, 1118
Ecl136II GAGCTC 1 cut(s) 452
EclXI CGGCCG 1 cut(s) 288
Eco130I CCWWGG 2 cut(s) 626, 1118
Eco147I AGGCCT 2 cut(s) 625, 1105
Eco24I GRGCYC 1 cut(s) 454
Eco31I GGTCTC 1 cut(s) 774
Eco47I GGWCC 1 cut(s) 1080
Eco52I CGGCCG 1 cut(s) 288
Eco53kI GAGCTC 1 cut(s) 452
Eco57I CTGAAG 2 cut(s) 736, 866
EcoICRI GAGCTC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 1080
EcoRII CCWGG 5 cut(s) 97, 212, 434, 764, 865
EcoT14I CCWWGG 2 cut(s) 626, 1118
EcoT22I ATGCAT 1 cut(s) 1185
EcoT38I GRGCYC 1 cut(s) 454
ErhI CCWWGG 2 cut(s) 626, 1118
FaeI CATG 3 cut(s) 170, 530, 840
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 2 cut(s) 155, 1066
FatI CATG 3 cut(s) 166, 526, 836
FokI GGATG 1 cut(s) 52
FriOI GRGCYC 1 cut(s) 454
FspBI CTAG 2 cut(s) 627, 1119
GlaI GCGC 1 cut(s) 444
HaeIII GGCC 8 cut(s) 97, 290, 434, 625, 873, 953, 1070, 1105
HapII CCGG 1 cut(s) 173
HgaI GACGC 1 cut(s) 308
HhaI GCGC 1 cut(s) 445
Hin1II CATG 3 cut(s) 170, 530, 840
Hin6I GCGC 1 cut(s) 443
HinP1I GCGC 1 cut(s) 443
HinfI GANTC 3 cut(s) 206, 406, 777
HpaII CCGG 1 cut(s) 173
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 3 cut(s) 924, 983, 1091
Hpy188I TCNGA 7 cut(s) 108, 221, 411, 422, 566, 755, 796
Hpy188III TCNNGA 4 cut(s) 62, 502, 884, 1216
Hpy8I GTNNAC 3 cut(s) 924, 983, 1091
Hpy99I CGWCG 1 cut(s) 585
HpyAV CCTTC 6 cut(s) 19, 114, 238, 433, 711, 760
HpyCH4III ACNGT 5 cut(s) 415, 787, 862, 1088, 1163
HpyCH4IV ACGT 2 cut(s) 580, 810
HpyCH4V TGCA 8 cut(s) 152, 475, 535, 689, 918, 974, 1091, 1183
HpyF3I CTNAG 4 cut(s) 208, 218, 318, 455
HpySE526I ACGT 2 cut(s) 580, 810
Hsp92II CATG 3 cut(s) 170, 530, 840
HspAI GCGC 1 cut(s) 443
Kzo9I GATC 4 cut(s) 103, 221, 732, 761
LweI GCATC 2 cut(s) 139, 1192
MaeI CTAG 2 cut(s) 627, 1119
MaeII ACGT 2 cut(s) 580, 810
MalI GATC 4 cut(s) 105, 223, 734, 763
MboI GATC 4 cut(s) 103, 221, 732, 761
MboII GAAGA 8 cut(s) 55, 127, 173, 515, 816, 818, 1135, 1220
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 2 cut(s) 221, 761
MhlI GDGCHC 4 cut(s) 454, 553, 580, 1093
MlsI TGGCCA 2 cut(s) 434, 953
MluCI AATT 5 cut(s) 159, 264, 665, 843, 1219
MluNI TGGCCA 2 cut(s) 434, 953
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 872
Mox20I TGGCCA 2 cut(s) 434, 953
Mph1103I ATGCAT 1 cut(s) 1185
MroXI GAANNNNTTC 1 cut(s) 605
MscI TGGCCA 2 cut(s) 434, 953
MseI TTAA 3 cut(s) 468, 708, 1065
MslI CAYNNNNRTG 2 cut(s) 835, 1178
Msp20I TGGCCA 2 cut(s) 434, 953
MspA1I CMGCKG 1 cut(s) 293
MspI CCGG 1 cut(s) 173
MspR9I CCNGG 6 cut(s) 99, 173, 214, 436, 766, 867
MunI CAATTG 1 cut(s) 264
Mva1269I GAATGC 3 cut(s) 920, 1173, 1185
MvaI CCWGG 5 cut(s) 99, 214, 436, 766, 867
NciI CCSGG 1 cut(s) 173
NdeII GATC 4 cut(s) 103, 221, 732, 761
NlaIII CATG 3 cut(s) 170, 530, 840
NlaIV GGNNCC 3 cut(s) 608, 872, 1082
NmeAIII GCCGAG 2 cut(s) 73, 259
NotI GCGGCCGC 1 cut(s) 288
NsiI ATGCAT 1 cut(s) 1185
NspV TTCGAA 1 cut(s) 1177
PaqCI CACCTGC 1 cut(s) 994
PceI AGGCCT 2 cut(s) 625, 1105
PcsI WCGNNNNNNNCGW 2 cut(s) 741, 1183
PctI GAATGC 3 cut(s) 920, 1173, 1185
PdmI GAANNNNTTC 1 cut(s) 605
PfeI GAWTC 2 cut(s) 406, 777
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PpuMI RGGWCCY 1 cut(s) 1080
PshAI GACNNNNGTC 1 cut(s) 560
Psp124BI GAGCTC 1 cut(s) 454
Psp5II RGGWCCY 1 cut(s) 1080
Psp6I CCWGG 5 cut(s) 97, 212, 434, 764, 865
PspGI CCWGG 5 cut(s) 97, 212, 434, 764, 865
PspN4I GGNNCC 3 cut(s) 608, 872, 1082
PspPI GGNCC 2 cut(s) 871, 1080
PspPPI RGGWCCY 1 cut(s) 1080
PsuI RGATCY 2 cut(s) 221, 761
RsaI GTAC 5 cut(s) 79, 864, 899, 940, 982
RsaNI GTAC 5 cut(s) 78, 863, 898, 939, 981
RseI CAYNNNNRTG 2 cut(s) 835, 1178
SacI GAGCTC 1 cut(s) 454
SaqAI TTAA 3 cut(s) 468, 708, 1065
Sau3AI GATC 4 cut(s) 103, 221, 732, 761
Sau96I GGNCC 2 cut(s) 871, 1080
ScaI AGTACT 1 cut(s) 79
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 6 cut(s) 99, 173, 214, 436, 766, 867
SduI GDGCHC 4 cut(s) 454, 553, 580, 1093
SfaNI GCATC 2 cut(s) 139, 1192
SfcI CTRYAG 1 cut(s) 927
SfuI TTCGAA 1 cut(s) 1177
SinI GGWCC 1 cut(s) 1080
SmiMI CAYNNNNRTG 2 cut(s) 835, 1178
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 5 cut(s) 159, 264, 665, 843, 1219
SseBI AGGCCT 2 cut(s) 625, 1105
SsiI CCGC 7 cut(s) 269, 287, 291, 323, 326, 332, 395
SspMI CTAG 2 cut(s) 627, 1119
SstI GAGCTC 1 cut(s) 454
StuI AGGCCT 2 cut(s) 625, 1105
StyD4I CCNGG 6 cut(s) 97, 171, 212, 434, 764, 865
StyI CCWWGG 2 cut(s) 626, 1118
TaaI ACNGT 5 cut(s) 415, 787, 862, 1088, 1163
TaiI ACGT 2 cut(s) 583, 813
TaqI TCGA 3 cut(s) 744, 1177, 1186
TaqII GACCGA 1 cut(s) 954
TasI AATT 5 cut(s) 159, 264, 665, 843, 1219
TatI WGTACW 2 cut(s) 77, 980
TauI GCSGC 4 cut(s) 290, 293, 326, 398
TfiI GAWTC 2 cut(s) 406, 777
Tru1I TTAA 3 cut(s) 468, 708, 1065
Tru9I TTAA 3 cut(s) 468, 708, 1065
TscAI CASTG 6 cut(s) 289, 790, 837, 910, 1093, 1168
TspDTI ATGAA 8 cut(s) 56, 515, 666, 711, 735, 906, 927, 1155
TspRI CASTG 6 cut(s) 289, 790, 837, 910, 1093, 1168
VneI GTGCAC 1 cut(s) 1089
VpaK11BI GGWCC 1 cut(s) 1080
XapI RAATTY 1 cut(s) 665
XmaJI CCTAGG 2 cut(s) 626, 1118
XmnI GAANNNNTTC 1 cut(s) 605
XspI CTAG 2 cut(s) 627, 1119
ZrmI AGTACT 1 cut(s) 79
Zsp2I ATGCAT 1 cut(s) 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.