Rroxscaffold_2G00149540

Protein of unknown function (DUF4005)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002379
Physical Location & Seq
Reverse (-)
87093861 .. 87095788
1928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149540.1

Sequence Viewer

Length: 1326 bp
ATGGGTAGAGCAACGAGGTGGCTTAAGGGCTTGTTTGGGATTAAGAGGGACAAAGAAAGAGAGCACCACCAGAGACCCAATTCAGATTCAGCTTTGGTTTCCTGTTTTGAATACAAGAAAGCTTCAAGCTTTGGGAGGGAGGCGAGTCCTCCTCCGGCGACGACTCTGCTGTGTCATAATCCGGCCACTATCCCGCCGAACATTTCACCGTCCGAGGCGGCCTGGCTGAGGTCCTTCTACAATGAAACAGAGAATGAACAGAGCAAGCACGCAATTGCAGTTGCGGCCGCCACGGCCGCCGCTGCTGATGCCGCAGTTGCTGCGGCTCAGGCGGCCGTGGCAGTCGTCCGCCTCACTAGCAATGGCAGAGGCACCATGTTCGGCGGCGGCCGGGAGAGATGGGCCGCCGTCAAGGTCCAAACTTGCTTCCGGGCATACTTGGCCAGAAAAGCCCTGAGAGCTCTGAAAGGACTGGTGAAGTTACAGGCATTAGTGAGAGGCTATTTAGTGAGGAAGCAAGCCACTGCTACACTCCACGGTATGCAAGCTCTGGTCAGAGCACAGGCCACTGTTCGTTCTCATAAATCCAGGGGCCTCAACCTCAACATTAACATCACTGAAGCCAACAGAATCGAAATGCGCGCACGAAAATCCACGGAGAGGTTTGATGAGAGTCGGAGCGAGCACACAGCTCCAAGCCACAGCAGGAGGCTCTCTGGTTGTTTGGAGAACAACAACAGTTTCAACACCATTGATGAAATTCCCAAGATTGTTGAGGTTGATACAGGAAGGCCTAAATCCAGGTCTCGCAGAACCAACACTTCCATTTCTGAACTAAGCGACGAATTTTACCAGGCAGTGTCTTCTCCACTCCCCTGTTGCTGTCCGGCCAGGTTGACCATCCCCGACCACCGGAACTTCCAAGAGTCCGACTGGGGACTCACCGGAGAGGAATGCAGGTTCTCCACGGCGCAGAGCACGCCGAGATTTGTGAACTCCTGCGGATCTAATGCCCCGGCCACGCCGGCCAAGAGTGTCTGCGCCGACAACTTTTACAGGGGATTTGGGAACTACCCGAACTACATGGCGAGCACGCAGTCTTTCAAGGCCAAGTTGAGGTCTCACAGCGCTCCGAAACAGAGGCCGGAGCCGGGGACGAAGAAGAGGCTTTCGCTTAATGAGTTGCTCGAGTCAAGGAACAGTTTGAGTGGTGTTAAGATGCAGAGGTCTTGTTCACAAGTGCAGGAAGCCATTAATTTCAAGAATGCTGTGATGGGGAAGCTTGATAGGTCGTCAGATTTTAGTAGCTTGCTGAGGAGGTCTTAA

Protein Analysis

441

Amino Acids

48.67

Weight (kDa)

10.07

Isoelectric Point (pI)

59.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4005 PF13178 329 - 405 2.6e-14 Protein of unknown function (DUF4005)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 948
AccB1I GGYRCC 1 cut(s) 371
AccII CGCG 1 cut(s) 642
AclWI GGATC 1 cut(s) 1012
AcoI YGGCCR 9 cut(s) 183, 285, 294, 333, 388, 441, 888, 1017, 1026
AcsI RAATTY 2 cut(s) 759, 845
AcuI CTGAAG 1 cut(s) 639
AfeI AGCGCT 1 cut(s) 1129
AfiI CCNNNNNNNGG 5 cut(s) 228, 660, 912, 1116, 1151
AflII CTTAAG 1 cut(s) 23
AgsI TTSAA 5 cut(s) 110, 126, 745, 1105, 1261
AjnI CCWGG 5 cut(s) 221, 587, 800, 852, 890
AloI GAACNNNNNNTCC 2 cut(s) 944, 976
AluBI AGCT 8 cut(s) 92, 122, 129, 461, 548, 692, 1282, 1308
AluI AGCT 8 cut(s) 92, 122, 129, 461, 548, 692, 1282, 1308
Alw21I GWGCWC 6 cut(s) 66, 463, 562, 687, 980, 1094
Alw26I GTCTC 3 cut(s) 67, 810, 1125
AlwI GGATC 1 cut(s) 1012
AlwNI CAGNNNCTG 1 cut(s) 320
Ama87I CYCGRG 1 cut(s) 1187
Aor51HI AGCGCT 1 cut(s) 1129
ApeKI GCWGC 2 cut(s) 302, 320
ApoI RAATTY 2 cut(s) 759, 845
AseI ATTAAT 1 cut(s) 1254
AspLEI GCGC 5 cut(s) 642, 644, 973, 1043, 1130
AspS9I GGNCC 4 cut(s) 231, 402, 415, 592
AsuC2I CCSGG 4 cut(s) 392, 431, 1016, 1152
AsuHPI GGTGA 3 cut(s) 198, 487, 934
AvaI CYCGRG 1 cut(s) 1187
AvaII GGWCC 2 cut(s) 231, 415
BalI TGGCCA 1 cut(s) 443
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 855
Bbv12I GWGCWC 6 cut(s) 66, 463, 562, 687, 980, 1094
BbvCI CCTCAGC 2 cut(s) 227, 1313
BbvI GCAGC 2 cut(s) 289, 307
BccI CCATC 3 cut(s) 393, 908, 1267
BceAI ACGGC 4 cut(s) 309, 320, 392, 984
BcgI CGANNNNNNTGC 4 cut(s) 148, 182, 361, 395
BciT130I CCWGG 5 cut(s) 223, 589, 802, 854, 892
BcnI CCSGG 4 cut(s) 392, 431, 1016, 1152
BcoDI GTCTC 3 cut(s) 67, 810, 1125
BfaI CTAG 1 cut(s) 357
BfoI RGCGCY 1 cut(s) 1131
BfrI CTTAAG 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 948
BglI GCCNNNNNGGC 2 cut(s) 293, 1025
Bme1390I CCNGG 9 cut(s) 223, 392, 431, 589, 802, 854, 892, 1016, 1152
Bme18I GGWCC 2 cut(s) 231, 415
BmeT110I CYCGRG 1 cut(s) 1187
BmgT120I GGNCC 4 cut(s) 231, 402, 415, 592
BmiI GGNNCC 3 cut(s) 373, 593, 1149
BmrFI CCNGG 9 cut(s) 223, 392, 431, 589, 802, 854, 892, 1016, 1152
BmrI ACTGGG 1 cut(s) 943
BmsI GCATC 2 cut(s) 298, 1209
BmuI ACTGGG 1 cut(s) 943
BpiI GAAGAC 1 cut(s) 855
BplI GAGNNNNNCTC 2 cut(s) 136, 168
Bpu10I CCTNAGC 3 cut(s) 227, 327, 1313
BpuMI CCSGG 4 cut(s) 392, 431, 1016, 1152
BsaI GGTCTC 3 cut(s) 67, 810, 1125
BsaJI CCNNGG 9 cut(s) 213, 291, 336, 535, 588, 654, 966, 1014, 1151
BsaWI WCCGGW 2 cut(s) 912, 944
Bsc4I CCNNNNNNNGG 5 cut(s) 228, 660, 912, 1116, 1151
Bse118I RCCGGY 1 cut(s) 1024
Bse1I ACTGG 2 cut(s) 477, 938
Bse3DI GCAATG 1 cut(s) 367
BseBI CCWGG 5 cut(s) 223, 589, 802, 854, 892
BseDI CCNNGG 9 cut(s) 213, 291, 336, 535, 588, 654, 966, 1014, 1151
BseGI GGATG 1 cut(s) 900
BseLI CCNNNNNNNGG 5 cut(s) 228, 660, 912, 1116, 1151
BseMI GCAATG 1 cut(s) 367
BseMII CTCAG 4 cut(s) 218, 341, 446, 1304
BseNI ACTGG 2 cut(s) 477, 938
BsePI GCGCGC 1 cut(s) 640
BseRI GAGGAG 1 cut(s) 141
BseX3I CGGCCG 4 cut(s) 285, 294, 333, 388
BseXI GCAGC 2 cut(s) 289, 307
BsgI GTGCAG 1 cut(s) 1262
Bsh1236I CGCG 1 cut(s) 642
Bsh1285I CGRYCG 4 cut(s) 288, 297, 336, 391
BshNI GGYRCC 1 cut(s) 371
BsiEI CGRYCG 4 cut(s) 288, 297, 336, 391
BsiHKAI GWGCWC 6 cut(s) 66, 463, 562, 687, 980, 1094
BsiHKCI CYCGRG 1 cut(s) 1187
BslFI GGGAC 3 cut(s) 62, 951, 1168
BslI CCNNNNNNNGG 5 cut(s) 228, 660, 912, 1116, 1151
BsmAI GTCTC 3 cut(s) 67, 810, 1125
BsmFI GGGAC 3 cut(s) 62, 951, 1168
BsmI GAATGC 2 cut(s) 959, 1270
Bso31I GGTCTC 3 cut(s) 67, 810, 1125
BsoBI CYCGRG 1 cut(s) 1187
Bsp1286I GDGCHC 6 cut(s) 66, 463, 562, 687, 980, 1094
Bsp143I GATC 1 cut(s) 1004
BspCNI CTCAG 4 cut(s) 219, 340, 447, 1305
BspFNI CGCG 1 cut(s) 642
BspLI GGNNCC 3 cut(s) 373, 593, 1149
BspMI ACCTGC 1 cut(s) 948
BspPI GGATC 1 cut(s) 1012
BspT107I GGYRCC 1 cut(s) 371
BspTI CTTAAG 1 cut(s) 23
BspTNI GGTCTC 3 cut(s) 67, 810, 1125
BsrDI GCAATG 1 cut(s) 367
BsrFI RCCGGY 1 cut(s) 1024
BsrI ACTGG 2 cut(s) 477, 938
BssAI RCCGGY 1 cut(s) 1024
BssECI CCNNGG 9 cut(s) 213, 291, 336, 535, 588, 654, 966, 1014, 1151
BssHII GCGCGC 1 cut(s) 640
BssMI GATC 1 cut(s) 1004
Bst2UI CCWGG 5 cut(s) 223, 589, 802, 854, 892
Bst4CI ACNGT 5 cut(s) 210, 539, 571, 740, 1202
Bst6I CTCTTC 1 cut(s) 1157
BstAFI CTTAAG 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 320
BstDEI CTNAG 5 cut(s) 227, 327, 455, 836, 1313
BstDSI CCRYGG 5 cut(s) 291, 336, 535, 654, 966
BstENI CCTNNNNNAGG 1 cut(s) 226
BstF5I GGATG 1 cut(s) 900
BstFNI CGCG 1 cut(s) 642
BstH2I RGCGCY 1 cut(s) 1131
BstHHI GCGC 5 cut(s) 642, 644, 973, 1043, 1130
BstKTI GATC 1 cut(s) 1007
BstMAI GTCTC 3 cut(s) 67, 810, 1125
BstMBI GATC 1 cut(s) 1004
BstMCI CGRYCG 4 cut(s) 288, 297, 336, 391
BstNI CCWGG 5 cut(s) 223, 589, 802, 854, 892
BstSCI CCNGG 9 cut(s) 221, 390, 429, 587, 800, 852, 890, 1014, 1150
BstUI CGCG 1 cut(s) 642
BstV1I GCAGC 2 cut(s) 289, 307
BstV2I GAAGAC 1 cut(s) 855
BstX2I RGATCY 1 cut(s) 1004
BstYI RGATCY 1 cut(s) 1004
BstZI CGGCCG 4 cut(s) 285, 294, 333, 388
BtgI CCRYGG 5 cut(s) 291, 336, 535, 654, 966
BtsCI GGATG 1 cut(s) 900
BtsI GCAGTG 2 cut(s) 522, 864
BtsIMutI CAGTG 4 cut(s) 522, 567, 615, 864
BveI ACCTGC 1 cut(s) 948
CaiI CAGNNNCTG 1 cut(s) 320
CciNI GCGGCCGC 1 cut(s) 285
CfoI GCGC 5 cut(s) 642, 644, 973, 1043, 1130
Cfr10I RCCGGY 1 cut(s) 1024
Cfr13I GGNCC 4 cut(s) 231, 402, 415, 592
CviAII CATG 2 cut(s) 376, 1084
DdeI CTNAG 5 cut(s) 227, 327, 455, 836, 1313
DpnI GATC 1 cut(s) 1006
DpnII GATC 1 cut(s) 1004
EaeI YGGCCR 9 cut(s) 183, 285, 294, 333, 388, 441, 888, 1017, 1026
EagI CGGCCG 4 cut(s) 285, 294, 333, 388
Eam1104I CTCTTC 1 cut(s) 1157
EarI CTCTTC 1 cut(s) 1157
EciI GGCGGA 1 cut(s) 338
Ecl136II GAGCTC 1 cut(s) 461
EclXI CGGCCG 4 cut(s) 285, 294, 333, 388
Eco147I AGGCCT 1 cut(s) 793
Eco24I GRGCYC 1 cut(s) 463
Eco31I GGTCTC 3 cut(s) 67, 810, 1125
Eco47I GGWCC 2 cut(s) 231, 415
Eco47III AGCGCT 1 cut(s) 1129
Eco52I CGGCCG 4 cut(s) 285, 294, 333, 388
Eco53kI GAGCTC 1 cut(s) 461
Eco57I CTGAAG 1 cut(s) 639
Eco88I CYCGRG 1 cut(s) 1187
EcoICRI GAGCTC 1 cut(s) 461
EcoNI CCTNNNNNAGG 1 cut(s) 226
EcoO109I RGGNCCY 2 cut(s) 231, 592
EcoRII CCWGG 5 cut(s) 221, 587, 800, 852, 890
EcoT38I GRGCYC 1 cut(s) 463
FaeI CATG 2 cut(s) 379, 1087
FaiI YATR 6 cut(s) 177, 377, 436, 542, 582, 1085
FaqI GGGAC 3 cut(s) 62, 951, 1168
FatI CATG 2 cut(s) 375, 1083
FauI CCCGC 1 cut(s) 201
FokI GGATG 1 cut(s) 887
FriOI GRGCYC 1 cut(s) 463
FspBI CTAG 1 cut(s) 357
GlaI GCGC 5 cut(s) 641, 643, 972, 1042, 1129
HaeII RGCGCY 1 cut(s) 1131
HhaI GCGC 5 cut(s) 642, 644, 973, 1043, 1130
Hin1II CATG 2 cut(s) 379, 1087
Hin6I GCGC 5 cut(s) 640, 642, 971, 1041, 1128
HinP1I GCGC 5 cut(s) 640, 642, 971, 1041, 1128
HincII GTYRAC 1 cut(s) 897
HindII GTYRAC 1 cut(s) 897
HindIII AAGCTT 3 cut(s) 120, 127, 1280
HinfI GANTC 8 cut(s) 86, 145, 163, 630, 673, 926, 939, 1190
HphI GGTGA 3 cut(s) 198, 487, 934
Hpy166II GTNNAC 3 cut(s) 897, 994, 1235
Hpy188I TCNGA 9 cut(s) 85, 214, 465, 557, 678, 832, 931, 1134, 1297
Hpy188III TCNNGA 1 cut(s) 1261
Hpy8I GTNNAC 3 cut(s) 897, 994, 1235
Hpy99I CGWCG 2 cut(s) 163, 845
HpyAV CCTTC 2 cut(s) 244, 783
HpyCH4III ACNGT 5 cut(s) 210, 539, 571, 740, 1202
HpyCH4V TGCA 5 cut(s) 278, 544, 957, 1222, 1243
HpyF3I CTNAG 5 cut(s) 227, 327, 455, 836, 1313
Hsp92II CATG 2 cut(s) 379, 1087
HspAI GCGC 5 cut(s) 640, 642, 971, 1041, 1128
KroI GCCGGC 1 cut(s) 1024
KroNI GCCGGC 1 cut(s) 1026
Kzo9I GATC 1 cut(s) 1004
LmnI GCTCC 4 cut(s) 678, 697, 1135, 1147
Lsp1109I GCAGC 2 cut(s) 289, 307
LweI GCATC 2 cut(s) 298, 1209
MaeI CTAG 1 cut(s) 357
MaeIII GTNAC 1 cut(s) 480
MalI GATC 1 cut(s) 1006
MboI GATC 1 cut(s) 1004
MboII GAAGA 3 cut(s) 855, 1171, 1174
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 1 cut(s) 1004
MhlI GDGCHC 6 cut(s) 66, 463, 562, 687, 980, 1094
MlsI TGGCCA 1 cut(s) 443
MluCI AATT 5 cut(s) 79, 273, 759, 845, 1255
MluNI TGGCCA 1 cut(s) 443
MlyI GAGTC 6 cut(s) 154, 157, 682, 933, 935, 1199
MmeI TCCRAC 2 cut(s) 656, 954
Mox20I TGGCCA 1 cut(s) 443
MroNI GCCGGC 1 cut(s) 1024
MscI TGGCCA 1 cut(s) 443
MseI TTAA 7 cut(s) 24, 42, 609, 1176, 1215, 1254, 1324
Msp20I TGGCCA 1 cut(s) 443
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 1 cut(s) 23
MspR9I CCNGG 9 cut(s) 223, 392, 431, 589, 802, 854, 892, 1016, 1152
MunI CAATTG 1 cut(s) 273
Mva1269I GAATGC 2 cut(s) 959, 1270
MvaI CCWGG 5 cut(s) 223, 589, 802, 854, 892
MvnI CGCG 1 cut(s) 642
NaeI GCCGGC 1 cut(s) 1026
NciI CCSGG 4 cut(s) 392, 431, 1016, 1152
NdeII GATC 1 cut(s) 1004
NgoMIV GCCGGC 1 cut(s) 1024
NlaIII CATG 2 cut(s) 379, 1087
NlaIV GGNNCC 3 cut(s) 373, 593, 1149
NmeAIII GCCGAG 1 cut(s) 1008
NotI GCGGCCGC 1 cut(s) 285
PaeR7I CTCGAG 1 cut(s) 1187
PauI GCGCGC 1 cut(s) 640
PceI AGGCCT 1 cut(s) 793
PctI GAATGC 2 cut(s) 959, 1270
PdiI GCCGGC 1 cut(s) 1026
PfeI GAWTC 2 cut(s) 86, 630
PleI GAGTC 6 cut(s) 153, 157, 681, 933, 934, 1198
PpsI GAGTC 6 cut(s) 153, 157, 681, 933, 934, 1198
PpuMI RGGWCCY 1 cut(s) 231
PshBI ATTAAT 1 cut(s) 1254
Psp124BI GAGCTC 1 cut(s) 463
Psp5II RGGWCCY 1 cut(s) 231
Psp6I CCWGG 5 cut(s) 221, 587, 800, 852, 890
PspGI CCWGG 5 cut(s) 221, 587, 800, 852, 890
PspN4I GGNNCC 3 cut(s) 373, 593, 1149
PspPI GGNCC 4 cut(s) 231, 402, 415, 592
PspPPI RGGWCCY 1 cut(s) 231
PspXI VCTCGAGB 1 cut(s) 1187
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 1 cut(s) 1004
PteI GCGCGC 1 cut(s) 640
SacI GAGCTC 1 cut(s) 463
SaqAI TTAA 7 cut(s) 24, 42, 609, 1176, 1215, 1254, 1324
Sau3AI GATC 1 cut(s) 1004
Sau96I GGNCC 4 cut(s) 231, 402, 415, 592
SchI GAGTC 6 cut(s) 154, 157, 682, 933, 935, 1199
ScrFI CCNGG 9 cut(s) 223, 392, 431, 589, 802, 854, 892, 1016, 1152
SduI GDGCHC 6 cut(s) 66, 463, 562, 687, 980, 1094
SfaNI GCATC 2 cut(s) 298, 1209
SfiI GGCCNNNNNGGCC 2 cut(s) 293, 1025
Sfr274I CTCGAG 1 cut(s) 1187
SinI GGWCC 2 cut(s) 231, 415
SlaI CTCGAG 1 cut(s) 1187
SmlI CTYRAG 2 cut(s) 23, 1187
SmoI CTYRAG 2 cut(s) 23, 1187
Sse9I AATT 5 cut(s) 79, 273, 759, 845, 1255
SseBI AGGCCT 1 cut(s) 793
SspMI CTAG 1 cut(s) 357
SstI GAGCTC 1 cut(s) 463
StuI AGGCCT 1 cut(s) 793
StyD4I CCNGG 9 cut(s) 221, 390, 429, 587, 800, 852, 890, 1014, 1150
TaaI ACNGT 5 cut(s) 210, 539, 571, 740, 1202
TaqI TCGA 2 cut(s) 633, 1188
TasI AATT 5 cut(s) 79, 273, 759, 845, 1255
TfiI GAWTC 2 cut(s) 86, 630
Tru1I TTAA 7 cut(s) 24, 42, 609, 1176, 1215, 1254, 1324
Tru9I TTAA 7 cut(s) 24, 42, 609, 1176, 1215, 1254, 1324
TscAI CASTG 4 cut(s) 529, 574, 622, 864
TseI GCWGC 2 cut(s) 302, 320
TspDTI ATGAA 3 cut(s) 258, 270, 771
TspGWI ACGGA 1 cut(s) 671
TspRI CASTG 4 cut(s) 529, 574, 622, 864
Vha464I CTTAAG 1 cut(s) 23
VpaK11BI GGWCC 2 cut(s) 231, 415
VspI ATTAAT 1 cut(s) 1254
XagI CCTNNNNNAGG 1 cut(s) 226
XapI RAATTY 2 cut(s) 759, 845
XhoI CTCGAG 1 cut(s) 1187
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.