FvH4_1g16120

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0003685
Physical Location & Seq
Forward (+)
9219208 .. 9220619
1412 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16120.t1

Sequence Viewer

Length: 1071 bp
ATGGTCGCCTGCGCGACCGGAGAAGTGCTGGATCTGCAGGCGTTGCCGGAAGGGTGCATAGCCAACATCATCTCATTCACAACACCACGAGATGCATGCAGGCTGTCCTCGGTGTCCAAGATTTTCAGGTCGGCCGCAGAATGTGACACAGTCTGGGACAAGTTTCTTCCCACTGATATACGTACGATCATATCCTGTTCTGTGTCTTCCTCTTCAGTTTCTTGGATCAAATCCAAGAAGGAGCTGTACCTTCGTCTCTGTGACAATCCAATCAGCACAGGAAATATAAGCTTTTCCCTGGACAAGGGGAGCGGGAAAAAATGCTATATGATATCTGCCAGGGCCCTTCAGATACTTGGGTCCCGTACTACTCATTTTTGGAGATGGATTTCTCTACCCGACTCCAGATTTGGGGAGGTGGCTGAGCTTGTCGATTTTTTGTGGCTCGAAATCACTGGGAAAATTGCTACATGGATTCTGTCCCCATCAACCCTATATAAAGCTTACCTTGTCTTCAAGTTAACTGAGCAAGCTTATGGATTTAGAGGACAACCTGTGAAGGTCAAAGTAAAAAAGTCTTATAAGTTGAATCCAACAACAGTGGGAATAGAAGCCAATGAGCAGTTTGTGGTTTTGGACCGAGAGAAAAAGAGACACTACTGGCAATGCATTTTGGGTATGCAAGCGTGGCAGCCTCGAGAAATCGATGAAGCTCCATATCCAAAGCAAAGAAAGGATGGCTGGTTGGAGATTGAGCTGGGTGAATTCTGTTATGAAAGAGATGAGGAGTTGCTAAAGATGTCTTGCAAGGAAAATAGTTGTGGTGCCTTCAAGGGTGGTCTCATTGTTCAAGGGATTGAGATCAGACCCTATTTTGGAGAAAGGCCGGATCCAAGGCAAAATTGGCGATCTTCTTGGGCCAGATCTTGGAACAAAAGGGCCCAATCTTACTCTTATTGGGTCCTATATTCCTCGTCTCTGACGTTCTTAATATTAGTTTTGCAATTTGCATTCCTCTTACCAATGTGGGTGTGCTCAACCGAAGATTCTACGATGCATTTGACAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0010154 GO:0022414 GO:0030246 GO:0032501 GO:0032502 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0061458
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box PP2

Protein Analysis

357

Amino Acids

40.86

Weight (kDa)

8.72

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2 PF14299 106 - 290 1.3e-49 Phloem protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 582
AccB1I GGYRCC 1 cut(s) 824
AccB7I CCANNNNNTGG 1 cut(s) 927
AccBSI CCGCTC 1 cut(s) 312
AccII CGCG 1 cut(s) 14
AciI CCGC 2 cut(s) 135, 312
AclWI GGATC 4 cut(s) 39, 233, 884, 897
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 764
AcuI CTGAAG 2 cut(s) 198, 332
AfaI GTAC 3 cut(s) 184, 248, 367
AfiI CCNNNNNNNGG 4 cut(s) 304, 411, 875, 927
AgsI TTSAA 4 cut(s) 517, 589, 832, 851
AjnI CCWGG 2 cut(s) 297, 338
AluBI AGCT 7 cut(s) 244, 291, 427, 503, 533, 713, 757
AluI AGCT 7 cut(s) 244, 291, 427, 503, 533, 713, 757
Alw21I GWGCWC 1 cut(s) 1037
Alw26I GTCTC 4 cut(s) 260, 646, 845, 981
AlwI GGATC 4 cut(s) 39, 233, 884, 897
Ama87I CYCGRG 1 cut(s) 696
AoxI GGCC 5 cut(s) 132, 342, 884, 918, 939
ApaI GGGCCC 2 cut(s) 346, 943
ApeKI GCWGC 1 cut(s) 691
ApoI RAATTY 1 cut(s) 764
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 8 cut(s) 342, 343, 360, 637, 918, 939, 940, 961
AsuHPI GGTGA 1 cut(s) 773
AvaI CYCGRG 1 cut(s) 696
AvaII GGWCC 3 cut(s) 360, 637, 961
BaeGI GKGCMC 2 cut(s) 346, 943
BaeI ACNNNNGTAYC 2 cut(s) 344, 377
BamHI GGATCC 1 cut(s) 889
BanI GGYRCC 1 cut(s) 824
BanII GRGCYC 2 cut(s) 346, 943
BarI GAAGNNNNNNTAC 2 cut(s) 230, 262
BauI CACGAG 1 cut(s) 87
BbsI GAAGAC 2 cut(s) 198, 505
Bbv12I GWGCWC 1 cut(s) 1037
BbvI GCAGC 1 cut(s) 703
BccI CCATC 3 cut(s) 378, 493, 731
BcgI CGANNNNNNTGC 2 cut(s) 78, 112
BciT130I CCWGG 2 cut(s) 299, 340
BcoDI GTCTC 4 cut(s) 260, 646, 845, 981
BfmI CTRYAG 1 cut(s) 35
BglII AGATCT 1 cut(s) 923
BisI GCNGC 2 cut(s) 135, 692
BlpI GCTNAGC 1 cut(s) 423
BlsI GCNGC 2 cut(s) 136, 693
Bme1390I CCNGG 2 cut(s) 299, 340
Bme18I GGWCC 3 cut(s) 360, 637, 961
BmeT110I CYCGRG 1 cut(s) 696
BmgT120I GGNCC 8 cut(s) 342, 343, 360, 637, 918, 939, 940, 961
BmiI GGNNCC 7 cut(s) 344, 361, 362, 826, 891, 941, 962
BmrFI CCNGG 2 cut(s) 299, 340
BmrI ACTGGG 1 cut(s) 465
BmsI GCATC 2 cut(s) 82, 1044
BmuI ACTGGG 1 cut(s) 465
BpiI GAAGAC 2 cut(s) 198, 505
BpmI CTGGAG 1 cut(s) 388
Bpu1102I GCTNAGC 1 cut(s) 423
Bsa29I ATCGAT 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 860
BsaI GGTCTC 1 cut(s) 845
BsaJI CCNNGG 4 cut(s) 108, 297, 339, 893
BsaWI WCCGGW 1 cut(s) 17
Bsc4I CCNNNNNNNGG 4 cut(s) 304, 411, 875, 927
Bse1I ACTGG 2 cut(s) 460, 665
Bse3DI GCAATG 1 cut(s) 671
Bse8I GATNNNNATC 1 cut(s) 860
BseBI CCWGG 2 cut(s) 299, 340
BseCI ATCGAT 1 cut(s) 705
BseDI CCNNGG 4 cut(s) 108, 297, 339, 893
BseGI GGATG 1 cut(s) 742
BseJI GATNNNNATC 1 cut(s) 860
BseLI CCNNNNNNNGG 4 cut(s) 304, 411, 875, 927
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 2 cut(s) 414, 516
BseNI ACTGG 2 cut(s) 460, 665
BseRI GAGGAG 1 cut(s) 800
BseSI GKGCMC 2 cut(s) 346, 943
BseX3I CGGCCG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 703
BseYI CCCAGC 1 cut(s) 757
Bsh1236I CGCG 1 cut(s) 14
Bsh1285I CGRYCG 2 cut(s) 18, 135
BshFI GGCC 5 cut(s) 134, 344, 886, 920, 941
BshNI GGYRCC 1 cut(s) 824
BshVI ATCGAT 1 cut(s) 705
BsiEI CGRYCG 2 cut(s) 18, 135
BsiHKAI GWGCWC 1 cut(s) 1037
BsiHKCI CYCGRG 1 cut(s) 696
BsiSI CCGG 3 cut(s) 18, 47, 887
BsiWI CGTACG 1 cut(s) 182
BslFI GGGAC 3 cut(s) 170, 346, 466
BslI CCNNNNNNNGG 4 cut(s) 304, 411, 875, 927
BsmAI GTCTC 4 cut(s) 260, 646, 845, 981
BsmBI CGTCTC 2 cut(s) 260, 981
BsmFI GGGAC 3 cut(s) 170, 346, 466
BsmI GAATGC 1 cut(s) 1010
BsnI GGCC 5 cut(s) 134, 344, 886, 920, 941
Bso31I GGTCTC 1 cut(s) 845
BsoBI CYCGRG 1 cut(s) 696
Bsp120I GGGCCC 2 cut(s) 342, 939
Bsp1286I GDGCHC 3 cut(s) 346, 943, 1037
Bsp143I GATC 7 cut(s) 31, 186, 225, 861, 889, 908, 923
Bsp1720I GCTNAGC 1 cut(s) 423
BspACI CCGC 2 cut(s) 135, 312
BspANI GGCC 5 cut(s) 134, 344, 886, 920, 941
BspCNI CTCAG 2 cut(s) 415, 517
BspDI ATCGAT 1 cut(s) 705
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 7 cut(s) 344, 361, 362, 826, 891, 941, 962
BspMAI CTGCAG 1 cut(s) 39
BspPI GGATC 4 cut(s) 39, 233, 884, 897
BspT107I GGYRCC 1 cut(s) 824
BspTNI GGTCTC 1 cut(s) 845
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 671
BsrI ACTGG 2 cut(s) 460, 665
BssECI CCNNGG 4 cut(s) 108, 297, 339, 893
BssMI GATC 7 cut(s) 31, 186, 225, 861, 889, 908, 923
BssSI CACGAG 1 cut(s) 87
BssT1I CCWWGG 1 cut(s) 893
Bst2BI CACGAG 1 cut(s) 87
Bst2UI CCWGG 2 cut(s) 299, 340
Bst4CI ACNGT 2 cut(s) 151, 601
Bst6I CTCTTC 1 cut(s) 217
BstAPI GCANNNNNTGC 1 cut(s) 43
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 6 cut(s) 10, 39, 97, 101, 531, 684
BstDEI CTNAG 2 cut(s) 423, 525
BstENI CCTNNNNNAGG 1 cut(s) 302
BstF5I GGATG 1 cut(s) 742
BstFNI CGCG 1 cut(s) 14
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 7 cut(s) 34, 189, 228, 864, 892, 911, 926
BstMAI GTCTC 4 cut(s) 260, 646, 845, 981
BstMBI GATC 7 cut(s) 31, 186, 225, 861, 889, 908, 923
BstMCI CGRYCG 2 cut(s) 18, 135
BstMWI GCNNNNNNNGC 4 cut(s) 34, 43, 688, 904
BstNI CCWGG 2 cut(s) 299, 340
BstNSI RCATGY 1 cut(s) 99
BstSCI CCNGG 2 cut(s) 297, 338
BstSFI CTRYAG 1 cut(s) 35
BstSLI GKGCMC 2 cut(s) 346, 943
BstSNI TACGTA 1 cut(s) 182
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 1 cut(s) 703
BstV2I GAAGAC 2 cut(s) 198, 505
BstX2I RGATCY 3 cut(s) 31, 889, 923
BstYI RGATCY 3 cut(s) 31, 889, 923
BstZI CGGCCG 1 cut(s) 132
Bsu15I ATCGAT 1 cut(s) 705
BsuRI GGCC 5 cut(s) 134, 344, 886, 920, 941
BsuTUI ATCGAT 1 cut(s) 705
BtsCI GGATG 1 cut(s) 742
BtsIMutI CAGTG 3 cut(s) 171, 453, 606
Cac8I GCNNGC 6 cut(s) 10, 39, 97, 101, 531, 684
CfoI GCGC 1 cut(s) 14
Cfr13I GGNCC 8 cut(s) 342, 343, 360, 637, 918, 939, 940, 961
ClaI ATCGAT 1 cut(s) 705
Csp6I GTAC 3 cut(s) 183, 247, 366
CspCI CAANNNNNGTGG 2 cut(s) 582, 617
CviAII CATG 2 cut(s) 96, 471
CviQI GTAC 3 cut(s) 183, 247, 366
DdeI CTNAG 2 cut(s) 423, 525
DpnI GATC 7 cut(s) 33, 188, 227, 863, 891, 910, 925
DpnII GATC 7 cut(s) 31, 186, 225, 861, 889, 908, 923
EaeI YGGCCR 1 cut(s) 132
EagI CGGCCG 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 217
EclXI CGGCCG 1 cut(s) 132
Eco105I TACGTA 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 893
Eco24I GRGCYC 2 cut(s) 346, 943
Eco31I GGTCTC 1 cut(s) 845
Eco32I GATATC 1 cut(s) 333
Eco47I GGWCC 3 cut(s) 360, 637, 961
Eco52I CGGCCG 1 cut(s) 132
Eco57I CTGAAG 2 cut(s) 198, 332
Eco88I CYCGRG 1 cut(s) 696
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoO109I RGGNCCY 5 cut(s) 342, 343, 360, 939, 961
EcoRI GAATTC 1 cut(s) 764
EcoRII CCWGG 2 cut(s) 297, 338
EcoRV GATATC 1 cut(s) 333
EcoT14I CCWWGG 1 cut(s) 893
EcoT22I ATGCAT 3 cut(s) 97, 671, 1059
EcoT38I GRGCYC 2 cut(s) 346, 943
ErhI CCWWGG 1 cut(s) 893
Esp3I CGTCTC 2 cut(s) 260, 981
FaeI CATG 2 cut(s) 99, 474
FalI AAGNNNNNCTT 2 cut(s) 492, 524
FaqI GGGAC 3 cut(s) 170, 346, 466
FatI CATG 2 cut(s) 95, 470
FauI CCCGC 1 cut(s) 305
Fnu4HI GCNGC 2 cut(s) 135, 692
FokI GGATG 1 cut(s) 749
FriOI GRGCYC 2 cut(s) 346, 943
Fsp4HI GCNGC 2 cut(s) 135, 692
GlaI GCGC 1 cut(s) 13
GluI GCNGC 2 cut(s) 135, 692
GsaI CCCAGC 1 cut(s) 761
GsuI CTGGAG 1 cut(s) 388
HaeIII GGCC 5 cut(s) 134, 344, 886, 920, 941
HapII CCGG 3 cut(s) 18, 47, 887
HhaI GCGC 1 cut(s) 14
Hin1II CATG 2 cut(s) 99, 474
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HincII GTYRAC 1 cut(s) 522
HindII GTYRAC 1 cut(s) 522
HindIII AAGCTT 3 cut(s) 289, 501, 531
HinfI GANTC 4 cut(s) 401, 475, 589, 1046
HpaI GTTAAC 1 cut(s) 522
HpaII CCGG 3 cut(s) 18, 47, 887
HphI GGTGA 1 cut(s) 773
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 3 cut(s) 351, 866, 981
Hpy188III TCNNGA 2 cut(s) 405, 698
Hpy8I GTNNAC 1 cut(s) 522
HpyAV CCTTC 6 cut(s) 44, 232, 260, 356, 553, 838
HpyCH4III ACNGT 2 cut(s) 151, 601
HpyCH4IV ACGT 2 cut(s) 181, 983
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 43, 688, 904
HpyF3I CTNAG 2 cut(s) 423, 525
HpySE526I ACGT 2 cut(s) 181, 983
Hsp92II CATG 2 cut(s) 99, 474
HspAI GCGC 1 cut(s) 12
KflI GGGWCCC 1 cut(s) 360
KspAI GTTAAC 1 cut(s) 522
Kzo9I GATC 7 cut(s) 31, 186, 225, 861, 889, 908, 923
LmnI GCTCC 3 cut(s) 241, 309, 718
Lsp1109I GCAGC 1 cut(s) 703
LweI GCATC 2 cut(s) 82, 1044
MaeII ACGT 2 cut(s) 181, 983
MaeIII GTNAC 2 cut(s) 143, 260
MalI GATC 7 cut(s) 33, 188, 227, 863, 891, 910, 925
MbiI CCGCTC 1 cut(s) 312
MboI GATC 7 cut(s) 31, 186, 225, 861, 889, 908, 923
MboII GAAGA 6 cut(s) 158, 198, 204, 505, 903, 1055
MflI RGATCY 3 cut(s) 31, 889, 923
MhlI GDGCHC 3 cut(s) 346, 943, 1037
MluCI AATT 5 cut(s) 462, 764, 901, 1004, 1066
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 2 cut(s) 617, 726
MnlI CCTC 8 cut(s) 118, 220, 409, 539, 705, 778, 982, 1025
Mph1103I ATGCAT 3 cut(s) 97, 671, 1059
MseI TTAA 2 cut(s) 521, 989
MspI CCGG 3 cut(s) 18, 47, 887
MspR9I CCNGG 2 cut(s) 299, 340
Mva1269I GAATGC 1 cut(s) 1010
MvaI CCWGG 2 cut(s) 299, 340
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 4 cut(s) 34, 43, 688, 904
NdeII GATC 7 cut(s) 31, 186, 225, 861, 889, 908, 923
NlaIII CATG 2 cut(s) 99, 474
NlaIV GGNNCC 7 cut(s) 344, 361, 362, 826, 891, 941, 962
NmuCI GTSAC 2 cut(s) 143, 260
NsiI ATGCAT 3 cut(s) 97, 671, 1059
NspI RCATGY 1 cut(s) 99
PaeI GCATGC 1 cut(s) 99
PaeR7I CTCGAG 1 cut(s) 696
PcsI WCGNNNNNNNCGW 1 cut(s) 980
PctI GAATGC 1 cut(s) 1010
PfeI GAWTC 3 cut(s) 475, 589, 1046
Pfl23II CGTACG 1 cut(s) 182
PflFI GACNNNGTC 1 cut(s) 149
PflMI CCANNNNNTGG 1 cut(s) 927
PkrI GCNGC 2 cut(s) 136, 693
PleI GAGTC 1 cut(s) 395
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 1 cut(s) 182
PpuMI RGGWCCY 2 cut(s) 360, 961
PsiI TTATAA 1 cut(s) 582
Psp5II RGGWCCY 2 cut(s) 360, 961
Psp6I CCWGG 2 cut(s) 297, 338
PspFI CCCAGC 1 cut(s) 757
PspGI CCWGG 2 cut(s) 297, 338
PspLI CGTACG 1 cut(s) 182
PspN4I GGNNCC 7 cut(s) 344, 361, 362, 826, 891, 941, 962
PspOMI GGGCCC 2 cut(s) 342, 939
PspPI GGNCC 8 cut(s) 342, 343, 360, 637, 918, 939, 940, 961
PspPPI RGGWCCY 2 cut(s) 360, 961
PstI CTGCAG 1 cut(s) 39
PsuI RGATCY 3 cut(s) 31, 889, 923
PsyI GACNNNGTC 1 cut(s) 149
RsaI GTAC 3 cut(s) 184, 248, 367
RsaNI GTAC 3 cut(s) 183, 247, 366
SaqAI TTAA 2 cut(s) 521, 989
SatI GCNGC 2 cut(s) 135, 692
Sau3AI GATC 7 cut(s) 31, 186, 225, 861, 889, 908, 923
Sau96I GGNCC 8 cut(s) 342, 343, 360, 637, 918, 939, 940, 961
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 2 cut(s) 299, 340
SduI GDGCHC 3 cut(s) 346, 943, 1037
SfaNI GCATC 2 cut(s) 82, 1044
SfcI CTRYAG 1 cut(s) 35
Sfr274I CTCGAG 1 cut(s) 696
SinI GGWCC 3 cut(s) 360, 637, 961
SlaI CTCGAG 1 cut(s) 696
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
SnaBI TACGTA 1 cut(s) 182
SphI GCATGC 1 cut(s) 99
Sse9I AATT 5 cut(s) 462, 764, 901, 1004, 1066
SsiI CCGC 2 cut(s) 135, 312
SspI AATATT 1 cut(s) 993
StyD4I CCNGG 2 cut(s) 297, 338
StyI CCWWGG 1 cut(s) 893
TaaI ACNGT 2 cut(s) 151, 601
TaiI ACGT 2 cut(s) 184, 986
TaqI TCGA 4 cut(s) 432, 447, 697, 705
TaqII GACCGA 1 cut(s) 654
TasI AATT 5 cut(s) 462, 764, 901, 1004, 1066
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 3 cut(s) 475, 589, 1046
Tru1I TTAA 2 cut(s) 521, 989
Tru9I TTAA 2 cut(s) 521, 989
TscAI CASTG 3 cut(s) 178, 460, 606
TseFI GTSAC 2 cut(s) 143, 260
TseI GCWGC 1 cut(s) 691
Tsp45I GTSAC 2 cut(s) 143, 260
TspDTI ATGAA 2 cut(s) 723, 789
TspRI CASTG 3 cut(s) 178, 460, 606
Tth111I GACNNNGTC 1 cut(s) 149
Van91I CCANNNNNTGG 1 cut(s) 927
VpaK11BI GGWCC 3 cut(s) 360, 637, 961
XagI CCTNNNNNAGG 1 cut(s) 302
XapI RAATTY 1 cut(s) 764
XceI RCATGY 1 cut(s) 99
XcmI CCANNNNNNNNNTGG 1 cut(s) 900
XhoI CTCGAG 1 cut(s) 696
Zsp2I ATGCAT 3 cut(s) 97, 671, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.