Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0002632

Physical Location & Seq

Genomic Coordinates Reverse (-)

16870497 .. 16880035

Gene Structure 9539 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g25070.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGAAGACCCAGATGAACAACCTCACTCTCCTCCTCCTCTTCCTCTTCACCTGTCACCTCTCCCAAGTCCTCTCATGGAAAAAAGACGAGTTCCGCAACTGTAACCAAACCCCCTTCTGCAAGCGCGCCCGGGCCCGCAAGCCCTCCTCCTCCTCCCTCGCCGCCCATGACGTCACCATCTCCGACGGCTCCCTCACCGCCAAACTCGTCTCCACCGGAATCGAACAAGACCAGGACCCGATCAGGCCCCTGGTCCTCACTCTCTCCGCCTACCACGACGGGATCCTCCGCCTTAAGATCGACGAGCTCGATCCGCCGCGGAAACGCTTCGAGGTCCCTGACGTCGTTTTACCTGAGTTCGAGGGGAAGAAGAAGCTCTGGCTGCAGCGGCTCTCGACGGAGACGATCGACAGCGCTCCGTCGTCGGTCGTGTTCTTATCCGACGGCTACGAGGCGGTGCTGCGGCACGATCCGTTCGAGGTCTACGTGCGCGAGACGAAGGGTAATCGTGTAATTTCCGTGAATTCGAATGGCTTGTTTGATTTTGAGCAATTGAGAGAGAATAAGAAAGAAGATGAGAATTGGGAAGAGAGGTTTAGAAGTCATACGGATAAGAGGCCGTACGGCCCTCAGTCCATTAGTTTTGATGTGTCGTTTTTCGGGGCTGATCATGTCTATGGGATCCCTGAGCGTGCCACAAGCTTTGCCTTGAAGCCTACTAGAGGCCCCGGGGTTGAGGAATCGGAGCCGTATAGGTTGTTCAACTTGGATGTGTTTGAGTATGTTCATGACTCACCATTCGGGCTATACGGCTCCATTCCGTTCATGATTTCCCACGGCAAGGCCCGGGGGACTTCCGGGTTCTTCTGGCTGAATGCTGCCGAAATGCAGATTGATGTGCTTGCGAAAGGGTGGGATGCCGAGGAAGGCATTGCTCTCCCTTCCTCGCAGGGGAGGATTGACACGTTTTGGATGAGTGAGGCTGGTGTTGTGGATGCTTTCTTTTTCGTGGGGCCGGGGCCGAAGGATGTGGCGCGGCAGTACACGAGTGTGACTGGCACGCCGTCAATGCCGCAGTTGTTTGCGGTGGCGTATCACCAATGTAGGTGGAACTATAGGGATGAGGAGGATGTGGAGCAAGTTGATGCTAAGTTTGATGAGTATGATATTCCGTATGATGTTTTGTGGCTTGATATTGAGCACACGGATGGGAAGAGGTATTTGACATGGGATAGGATGCTTTTCCCGCACCCGGAGGAGATGCAGAGGAAGTTGGCTGCTAAGGGTAGGCATATGGTTACTATTGTGGATCCTCATATTAAGAGAGATGACTCGTACTTTGTGCACAAGGAAGCCACAGAAAACGGCTATTATGTTAAGGATGCCAATGGGAATGATTATGATGGGTGGTGCTGGCCTGGTTCATCATCATATTTGGATATGTTGAGGCCAGAGGTTAGGTCCTGGTGGGCTACAAAGTTCTCTACTCAAAATTATGTTGGTTCGACTCCTTCCTTGTACATATGGAATGATATGAATGAGCCCTCTGTCTTCAATGGTCCCGAGGTCACAATGCCTAGAGATGCTGTACATCGAGGAGATGCAGAACACCGAGATGTACATAACGCCTATGGTTACTATTTTCACATGGCTACAGCAGATGGGCTTGTTAACCGAGGTGATGGAAGGGATAGGCCGTTTGTATTATCAAGAGCAGTATTTGCTGGAAGTCAAAGGTATGGAGCAGTATGGACGGGAGATAATACAGCTGAATGGGAACACCTTAGGGTGTCGGTTCCAATGGTTTTGACTCTTGGTCTTACTGGGATATCATTCTCTGGTGCTGATGTGGGTGGGTTTTTTGGCAATCCGGAACCTGAGTTATTAGTTCGTTGGTATCAGTTAGGAGCATACTATCCTTTCTTTAGAGCTCATGCTCATCATGACACCAAAAGACGAGAACCATGGTTGTTTGGAGATAAAAATACTGAACGTATAAGGGAGGCTATACACATGCGTTATATGTTGCTTCCATACTTTTACACATTGTTCAGAGAGGCAAACACCAGTGGTGTTCCTGTTGCTCGTCCATTGTGGATGGAGTTTCCTTCCGAGGAGGCTACTTTCACAAATGATGAGGCTCTTATGATTGGAAATAGTCTTTTGGTACAAGGGATCTATACAGAGCATGCAAAACATGCTTCAGTTTATTTGCCTGGGAAAGAATTATGGTATGATCTGAAAACGGGAGCAGCTTTCAAGGGAGGTAAGACCCATAAGCTGGATGTTAATGAGGAGGGTGTTCCTGCTTTCCAGAGAGCTGGGACTATCATACCAAGGAAAGACCGATTTCGTCGGAGTTCCACCCAGATGGTGAATGATCCATATACTCTGGTGATAGCACTGAATAGTTCACAAGCAGCCGAAGGTGAACTCTATGTGGATGACGGAAAAAGCTTTGAGTTTCAGCAAGGATCCTATATCCATCGTCGCTTTGTTTTCGCAGATGGGAAGCTTACATCTCTTAATCTGGCACTCGGACAAACTCAGTTTTCTTCAGAATGTGTTATCGAGAGGATTATTCTGCTAGGACTGTCTACTGGGCAAAAGAGTGCTACAATTGAACCAGCAAATCAAAATGCAGAAATTGAACGGGGGCCGCTTCTCCTTCATTCAAGGCAAGGACCAACAGCGTTAACGATCCGTAAGCCTAATGTCCGGATAGCAGATGATTGGGTCATAAAAATATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000902 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006491 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009250 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009987 GO:0010035 GO:0010038 GO:0012505 GO:0015926 GO:0016043 GO:0016049 GO:0016051 GO:0016787 GO:0016798 GO:0019538 GO:0030243 GO:0030244 GO:0030587 GO:0031288 GO:0032502 GO:0032989 GO:0033692 GO:0033919 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043687 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0051274 GO:0051703 GO:0051704 GO:0051707 GO:0060560 GO:0071704 GO:0071840 GO:0090599 GO:0090600 GO:0090702 GO:0098542 GO:0099120 GO:1901135 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

918

Amino Acids

104.53

Weight (kDa)

5.78

Isoelectric Point (pI)

38.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	86 - 299	1.1e-40	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	355 - 683	9.6e-122	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	691 - 778	1.4e-22	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63840 AT5G63840
fragaria_vesca	FvH4_1g25070
malus_domestica	MD01G1007800.v1.1 MD01G1007900.v1.1 MD15G1135800.v1.1
prunus_persica	Prupe.4G277800_v2.0.a1 Prupe.4G277800_v2.0.a1
pyrus_communis	pycom15g12240 pycom520g00330
rosa_chinensis	RchiOBHm_Chr2g0122711 RchiOBHm_Chr2g0122721
rosa_laevigata	RLG00000018707 RLG00000018709
rosa_multiflora	Rmu_sc0005370.1_g000002 Rmu_sc0008259.1_g000009 Rmu_sc0008259.1_g000014 Rmu_sc0017734.1_g000006 Rmu_sc0026486.1_g000001
rosa_roxburghii	Rroxscaffold_1G00031190 Rroxscaffold_2G00121270 Rroxscaffold_2G00121280 Rroxscaffold_4G00297410 Rroxscaffold_4G00317480 Rroxscaffold_4G00325980 Rroxscaffold_7G00197700 Rroxscaffold_7G00199130
rosa_rugosa	Rorug02G0241800 Rorug02G0241800 Rorug02G0241900 Rorug02G0242000 Rorug02G0242100
rosa_samantha	Rh2AG300300 Rh2BG308200 Rh2CG286400 Rh2CG286900 Rh2DG324500
rosa_wichuraiana	Rw2G023850 Rw2G023860 Rw2G024040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	174, 345
AccB7I	CCANNNNNTGG	1 cut(s)	2281
AccI	GTMKAC	2 cut(s)	483, 2597
AccII	CGCG	4 cut(s)	126, 319, 492, 1036
AccIII	TCCGGA	2 cut(s)	1870, 2718
AcsI	RAATTY	1 cut(s)	523
AcuI	CTGAAG	2 cut(s)	2187, 2541
AcyI	GRCGYC	2 cut(s)	171, 342
AfaI	GTAC	7 cut(s)	623, 1043, 1337, 1520, 1590, 1620, 2169
AfeI	AGCGCT	1 cut(s)	415
AfiI	CCNNNNNNNGG	5 cut(s)	722, 841, 951, 1252, 2281
AflII	CTTAAG	1 cut(s)	293
AflIII	ACRYGT	1 cut(s)	963
AgsI	TTSAA	7 cut(s)	712, 763, 1555, 2260, 2624, 2651, 2676
AhdI	GACNNNNNGTC	1 cut(s)	1815
AjnI	CCWGG	5 cut(s)	231, 249, 1417, 1463, 2215
AleI	CACNNNNGTG	1 cut(s)	1049
Alw21I	GWGCWC	4 cut(s)	309, 1203, 1347, 1933
Alw26I	GTCTC	3 cut(s)	214, 395, 488
Alw44I	GTGCAC	1 cut(s)	1343
Ama87I	CYCGRG	4 cut(s)	129, 728, 846, 1562
Aor13HI	TCCGGA	2 cut(s)	1870, 2718
Aor51HI	AGCGCT	1 cut(s)	415
ApaI	GGGCCC	1 cut(s)	136
ApaLI	GTGCAC	1 cut(s)	1343
ApeKI	GCWGC	7 cut(s)	382, 385, 460, 878, 1277, 2252, 2420
ApoI	RAATTY	1 cut(s)	523
ArsI	GACNNNNNNTTYG	4 cut(s)	782, 814, 1322, 1354
Asp700I	GAANNNNTTC	1 cut(s)	326
AspLEI	GCGC	5 cut(s)	126, 128, 416, 492, 1036
AsuC2I	CCSGG	9 cut(s)	130, 131, 729, 730, 847, 848, 859, 1017, 1253
AsuII	TTCGAA	1 cut(s)	527
AvaI	CYCGRG	4 cut(s)	129, 728, 846, 1562
AvaII	GGWCC	6 cut(s)	235, 253, 334, 1461, 1559, 2684
AxyI	CCTNAGG	1 cut(s)	1784
BaeGI	GKGCMC	2 cut(s)	136, 1347
BamHI	GGATCC	4 cut(s)	282, 681, 1309, 2474
BanII	GRGCYC	4 cut(s)	136, 309, 1545, 1933
BauI	CACGAG	1 cut(s)	1045
BbsI	GAAGAC	2 cut(s)	11, 1543
Bbv12I	GWGCWC	4 cut(s)	309, 1203, 1347, 1933
BbvI	GCAGC	7 cut(s)	369, 397, 447, 865, 1264, 2264, 2432
BccI	CCATC	9 cut(s)	185, 1202, 1397, 1655, 1676, 2092, 2365, 2493, 2501
BciT130I	CCWGG	5 cut(s)	233, 251, 1419, 1465, 2217
BclI	TGATCA	1 cut(s)	667
BcnI	CCSGG	9 cut(s)	130, 131, 729, 730, 847, 848, 859, 1017, 1253
BcoDI	GTCTC	3 cut(s)	214, 395, 488
BfaI	CTAG	3 cut(s)	720, 1578, 2588
BfmI	CTRYAG	3 cut(s)	383, 1114, 1653
BfoI	RGCGCY	1 cut(s)	417
BfrI	CTTAAG	1 cut(s)	293
Bme18I	GGWCC	6 cut(s)	235, 253, 334, 1461, 1559, 2684
BmeRI	GACNNNNNGTC	1 cut(s)	1815
BmeT110I	CYCGRG	4 cut(s)	129, 728, 846, 1562
BmrI	ACTGGG	2 cut(s)	1833, 2610
BmsI	GCATC	8 cut(s)	907, 985, 1135, 1227, 1251, 1372, 1573, 1591
BmuI	ACTGGG	2 cut(s)	1833, 2610
BpiI	GAAGAC	2 cut(s)	11, 1543
Bpu10I	CCTNAGC	2 cut(s)	687, 1281
Bpu14I	TTCGAA	1 cut(s)	527
BpuMI	CCSGG	9 cut(s)	130, 131, 729, 730, 847, 848, 859, 1017, 1253
BsaAI	YACGTR	1 cut(s)	487
BsaHI	GRCGYC	2 cut(s)	171, 342
BsaWI	WCCGGW	3 cut(s)	215, 1870, 2718
Bsc4I	CCNNNNNNNGG	5 cut(s)	722, 841, 951, 1252, 2281
Bse1I	ACTGG	4 cut(s)	1060, 1828, 2067, 2605
Bse21I	CCTNAGG	1 cut(s)	1784
Bse3DI	GCAATG	1 cut(s)	930
BseAI	TCCGGA	2 cut(s)	1870, 2718
BseBI	CCWGG	5 cut(s)	233, 251, 1419, 1465, 2217
BseLI	CCNNNNNNNGG	5 cut(s)	722, 841, 951, 1252, 2281
BseMI	GCAATG	1 cut(s)	930
BseMII	CTCAG	5 cut(s)	345, 644, 678, 1869, 2561
BseNI	ACTGG	4 cut(s)	1060, 1828, 2067, 2605
BsePI	GCGCGC	1 cut(s)	124
BseSI	GKGCMC	2 cut(s)	136, 1347
BseXI	GCAGC	7 cut(s)	369, 397, 447, 865, 1264, 2264, 2432
BseYI	CCCAGC	1 cut(s)	2321
Bsh1236I	CGCG	4 cut(s)	126, 319, 492, 1036
Bsh1285I	CGRYCG	2 cut(s)	408, 429
BsiEI	CGRYCG	2 cut(s)	408, 429
BsiHKAI	GWGCWC	4 cut(s)	309, 1203, 1347, 1933
BsiHKCI	CYCGRG	4 cut(s)	129, 728, 846, 1562
BsiSI	CCGG	9 cut(s)	130, 216, 729, 847, 858, 1016, 1253, 1871, 2719
BsiWI	CGTACG	1 cut(s)	621
BslFI	GGGAC	4 cut(s)	320, 865, 1545, 2338
BslI	CCNNNNNNNGG	5 cut(s)	722, 841, 951, 1252, 2281
BsmAI	GTCTC	3 cut(s)	214, 395, 488
BsmBI	CGTCTC	3 cut(s)	214, 395, 488
BsmFI	GGGAC	4 cut(s)	320, 865, 1545, 2338
BsmI	GAATGC	1 cut(s)	880
BsoBI	CYCGRG	4 cut(s)	129, 728, 846, 1562
Bsp119I	TTCGAA	1 cut(s)	527
Bsp120I	GGGCCC	1 cut(s)	132
Bsp1286I	GDGCHC	6 cut(s)	136, 309, 1203, 1347, 1545, 1933
Bsp13I	TCCGGA	2 cut(s)	1870, 2718
Bsp1407I	TGTACA	3 cut(s)	1518, 1588, 1618
Bsp19I	CCATGG	1 cut(s)	1964
BspCNI	CTCAG	5 cut(s)	346, 643, 679, 1870, 2560
BspEI	TCCGGA	2 cut(s)	1870, 2718
BspFNI	CGCG	4 cut(s)	126, 319, 492, 1036
BspHI	TCATGA	3 cut(s)	787, 825, 1942
BspMAI	CTGCAG	1 cut(s)	387
BspT104I	TTCGAA	1 cut(s)	527
BspTI	CTTAAG	1 cut(s)	293
BsrDI	GCAATG	1 cut(s)	930
BsrGI	TGTACA	3 cut(s)	1518, 1588, 1618
BsrI	ACTGG	4 cut(s)	1060, 1828, 2067, 2605
BssHII	GCGCGC	1 cut(s)	124
BssNI	GRCGYC	2 cut(s)	171, 342
BssSI	CACGAG	1 cut(s)	1045
BssT1I	CCWWGG	2 cut(s)	1964, 2336
Bst2BI	CACGAG	1 cut(s)	1045
Bst2UI	CCWGG	5 cut(s)	233, 251, 1419, 1465, 2217
Bst4CI	ACNGT	2 cut(s)	101, 2595
Bst6I	CTCTTC	4 cut(s)	44, 50, 582, 1208
BstACI	GRCGYC	2 cut(s)	171, 342
BstAFI	CTTAAG	1 cut(s)	293
BstAPI	GCANNNNNTGC	2 cut(s)	1721, 2198
BstAUI	TGTACA	3 cut(s)	1518, 1588, 1618
BstBAI	YACGTR	1 cut(s)	487
BstBI	TTCGAA	1 cut(s)	527
BstC8I	GCNNGC	9 cut(s)	122, 126, 136, 140, 693, 903, 1061, 1415, 2190
BstDEI	CTNAG	8 cut(s)	354, 630, 687, 1149, 1281, 1784, 1878, 2547
BstDSI	CCRYGG	3 cut(s)	317, 835, 1964
BstENI	CCTNNNNNAGG	1 cut(s)	720
BstFNI	CGCG	4 cut(s)	126, 319, 492, 1036
BstH2I	RGCGCY	1 cut(s)	417
BstHHI	GCGC	5 cut(s)	126, 128, 416, 492, 1036
BstMAI	GTCTC	3 cut(s)	214, 395, 488
BstMCI	CGRYCG	2 cut(s)	408, 429
BstMWI	GCNNNNNNNGC	7 cut(s)	313, 382, 388, 1069, 1246, 1721, 2198
BstNI	CCWGG	5 cut(s)	233, 251, 1419, 1465, 2217
BstNSI	RCATGY	3 cut(s)	2017, 2192, 2201
BstSFI	CTRYAG	3 cut(s)	383, 1114, 1653
BstSLI	GKGCMC	2 cut(s)	136, 1347
BstUI	CGCG	4 cut(s)	126, 319, 492, 1036
BstV1I	GCAGC	7 cut(s)	369, 397, 447, 865, 1264, 2264, 2432
BstV2I	GAAGAC	2 cut(s)	11, 1543
BstX2I	RGATCY	5 cut(s)	282, 681, 1309, 2175, 2474
BstXI	CCANNNNNNTGG	2 cut(s)	2321, 2371
BstYI	RGATCY	5 cut(s)	282, 681, 1309, 2175, 2474
Bsu36I	CCTNAGG	1 cut(s)	1784
BtgI	CCRYGG	3 cut(s)	317, 835, 1964
BtsIMutI	CAGTG	2 cut(s)	2074, 2402
Cac8I	GCNNGC	9 cut(s)	122, 126, 136, 140, 693, 903, 1061, 1415, 2190
CciI	TCATGA	3 cut(s)	787, 825, 1942
CfoI	GCGC	5 cut(s)	126, 128, 416, 492, 1036
Cfr42I	CCGCGG	1 cut(s)	320
Cfr9I	CCCGGG	3 cut(s)	129, 728, 846
Csp6I	GTAC	7 cut(s)	622, 1042, 1336, 1519, 1589, 1619, 2168
CspCI	CAANNNNNGTGG	4 cut(s)	685, 720, 1088, 1123
CviQI	GTAC	7 cut(s)	622, 1042, 1336, 1519, 1589, 1619, 2168
DdeI	CTNAG	8 cut(s)	354, 630, 687, 1149, 1281, 1784, 1878, 2547
DriI	GACNNNNNGTC	1 cut(s)	1815
Eam1104I	CTCTTC	4 cut(s)	44, 50, 582, 1208
Eam1105I	GACNNNNNGTC	1 cut(s)	1815
EarI	CTCTTC	4 cut(s)	44, 50, 582, 1208
EciI	GGCGGA	3 cut(s)	256, 278, 303
Ecl136II	GAGCTC	2 cut(s)	307, 1931
Eco130I	CCWWGG	2 cut(s)	1964, 2336
Eco24I	GRGCYC	4 cut(s)	136, 309, 1545, 1933
Eco32I	GATATC	1 cut(s)	1830
Eco47I	GGWCC	6 cut(s)	235, 253, 334, 1461, 1559, 2684
Eco47III	AGCGCT	1 cut(s)	415
Eco53kI	GAGCTC	2 cut(s)	307, 1931
Eco57I	CTGAAG	2 cut(s)	2187, 2541
Eco81I	CCTNAGG	1 cut(s)	1784
Eco88I	CYCGRG	4 cut(s)	129, 728, 846, 1562
EcoICRI	GAGCTC	2 cut(s)	307, 1931
EcoNI	CCTNNNNNAGG	1 cut(s)	720
EcoO109I	RGGNCCY	5 cut(s)	235, 246, 334, 725, 1461
EcoRI	GAATTC	1 cut(s)	523
EcoRII	CCWGG	5 cut(s)	231, 249, 1417, 1463, 2215
EcoRV	GATATC	1 cut(s)	1830
EcoT14I	CCWWGG	2 cut(s)	1964, 2336
EcoT38I	GRGCYC	4 cut(s)	136, 309, 1545, 1933
ErhI	CCWWGG	2 cut(s)	1964, 2336
Esp3I	CGTCTC	3 cut(s)	214, 395, 488
FaqI	GGGAC	4 cut(s)	320, 865, 1545, 2338
FauI	CCCGC	2 cut(s)	143, 1254
FauNDI	CATATG	2 cut(s)	1293, 1523
FbaI	TGATCA	1 cut(s)	667
FblI	GTMKAC	2 cut(s)	483, 2597
FriOI	GRGCYC	4 cut(s)	136, 309, 1545, 1933
FspBI	CTAG	3 cut(s)	720, 1578, 2588
GlaI	GCGC	5 cut(s)	125, 127, 415, 491, 1035
GsaI	CCCAGC	1 cut(s)	2325
HaeII	RGCGCY	1 cut(s)	417
HapII	CCGG	9 cut(s)	130, 216, 729, 847, 858, 1016, 1253, 1871, 2719
HhaI	GCGC	5 cut(s)	126, 128, 416, 492, 1036
Hin1I	GRCGYC	2 cut(s)	171, 342
Hin6I	GCGC	5 cut(s)	124, 126, 414, 490, 1034
HinP1I	GCGC	5 cut(s)	124, 126, 414, 490, 1034
HincII	GTYRAC	2 cut(s)	1672, 2697
HindII	GTYRAC	2 cut(s)	1672, 2697
HindIII	AAGCTT	3 cut(s)	700, 2455, 2513
HinfI	GANTC	6 cut(s)	219, 740, 791, 1331, 1507, 1810
HpaI	GTTAAC	2 cut(s)	1672, 2697
HpaII	CCGG	9 cut(s)	130, 216, 729, 847, 858, 1016, 1253, 1871, 2719
Hpy166II	GTNNAC	8 cut(s)	484, 1044, 1345, 1672, 2414, 2432, 2598, 2697
Hpy188I	TCNGA	9 cut(s)	184, 442, 745, 2054, 2113, 2241, 2358, 2540, 2560
Hpy8I	GTNNAC	8 cut(s)	484, 1044, 1345, 1672, 2414, 2432, 2598, 2697
HpyCH4III	ACNGT	2 cut(s)	101, 2595
HpyCH4IV	ACGT	5 cut(s)	171, 342, 486, 965, 1993
HpyCH4V	TGCA	8 cut(s)	120, 385, 889, 1264, 1345, 1604, 2192, 2642
HpyF10VI	GCNNNNNNNGC	7 cut(s)	313, 382, 388, 1069, 1246, 1721, 2198
HpyF3I	CTNAG	8 cut(s)	354, 630, 687, 1149, 1281, 1784, 1878, 2547
HpySE526I	ACGT	5 cut(s)	171, 342, 486, 965, 1993
Hsp92I	GRCGYC	2 cut(s)	171, 342
HspAI	GCGC	5 cut(s)	124, 126, 414, 490, 1034
Kpn2I	TCCGGA	2 cut(s)	1870, 2718
Ksp22I	TGATCA	1 cut(s)	667
KspAI	GTTAAC	2 cut(s)	1672, 2697
KspI	CCGCGG	1 cut(s)	320
LmnI	GCTCC	8 cut(s)	194, 421, 745, 818, 1135, 1742, 1907, 2249
Lsp1109I	GCAGC	7 cut(s)	369, 397, 447, 865, 1264, 2264, 2432
LweI	GCATC	8 cut(s)	907, 985, 1135, 1227, 1251, 1372, 1573, 1591
MaeI	CTAG	3 cut(s)	720, 1578, 2588
MaeII	ACGT	5 cut(s)	171, 342, 486, 965, 1993
MaeIII	GTNAC	7 cut(s)	53, 101, 172, 1051, 1297, 1567, 1634
MfeI	CAATTG	2 cut(s)	551, 2619
MflI	RGATCY	5 cut(s)	282, 681, 1309, 2175, 2474
MhlI	GDGCHC	6 cut(s)	136, 309, 1203, 1347, 1545, 1933
MluCI	AATT	8 cut(s)	513, 523, 551, 580, 1492, 2225, 2619, 2646
MlyI	GAGTC	4 cut(s)	785, 1325, 1501, 1804
MmeI	TCCRAC	3 cut(s)	207, 465, 2336
MroI	TCCGGA	2 cut(s)	1870, 2718
MroXI	GAANNNNTTC	1 cut(s)	326
MseI	TTAA	7 cut(s)	294, 1320, 1377, 1671, 2289, 2526, 2696
MslI	CAYNNNNRTG	5 cut(s)	675, 1049, 1206, 1614, 2369
MspA1I	CMGCKG	3 cut(s)	319, 388, 1769
MspCI	CTTAAG	1 cut(s)	293
MspI	CCGG	9 cut(s)	130, 216, 729, 847, 858, 1016, 1253, 1871, 2719
MunI	CAATTG	2 cut(s)	551, 2619
Mva1269I	GAATGC	1 cut(s)	880
MvaI	CCWGG	5 cut(s)	233, 251, 1419, 1465, 2217
MvnI	CGCG	4 cut(s)	126, 319, 492, 1036
MwoI	GCNNNNNNNGC	7 cut(s)	313, 382, 388, 1069, 1246, 1721, 2198
NciI	CCSGG	9 cut(s)	130, 131, 729, 730, 847, 848, 859, 1017, 1253
NcoI	CCATGG	1 cut(s)	1964
NdeI	CATATG	2 cut(s)	1293, 1523
NmeAIII	GCCGAG	1 cut(s)	946
NmuCI	GTSAC	4 cut(s)	53, 172, 1051, 1567
NspI	RCATGY	3 cut(s)	2017, 2192, 2201
NspV	TTCGAA	1 cut(s)	527
OliI	CACNNNNGTG	1 cut(s)	1049
PaeI	GCATGC	1 cut(s)	2192
PagI	TCATGA	3 cut(s)	787, 825, 1942
PauI	GCGCGC	1 cut(s)	124
PcsI	WCGNNNNNNNCGW	4 cut(s)	306, 401, 474, 483
PctI	GAATGC	1 cut(s)	880
PdmI	GAANNNNTTC	1 cut(s)	326
PfeI	GAWTC	2 cut(s)	219, 740
Pfl23II	CGTACG	1 cut(s)	621
PflMI	CCANNNNNTGG	1 cut(s)	2281
Ple19I	CGATCG	1 cut(s)	408
PleI	GAGTC	4 cut(s)	785, 1325, 1501, 1804
PpsI	GAGTC	4 cut(s)	785, 1325, 1501, 1804
Ppu21I	YACGTR	1 cut(s)	487
PpuMI	RGGWCCY	3 cut(s)	235, 334, 1461
Psp124BI	GAGCTC	2 cut(s)	309, 1933
Psp5II	RGGWCCY	3 cut(s)	235, 334, 1461
Psp6I	CCWGG	5 cut(s)	231, 249, 1417, 1463, 2215
PspFI	CCCAGC	1 cut(s)	2321
PspGI	CCWGG	5 cut(s)	231, 249, 1417, 1463, 2215
PspLI	CGTACG	1 cut(s)	621
PspOMI	GGGCCC	1 cut(s)	132
PspPPI	RGGWCCY	3 cut(s)	235, 334, 1461
PsrI	GAACNNNNNNTAC	2 cut(s)	743, 775
PstI	CTGCAG	1 cut(s)	387
PsuI	RGATCY	5 cut(s)	282, 681, 1309, 2175, 2474
PteI	GCGCGC	1 cut(s)	124
PvuI	CGATCG	1 cut(s)	408
PvuII	CAGCTG	1 cut(s)	1769
RsaI	GTAC	7 cut(s)	623, 1043, 1337, 1520, 1590, 1620, 2169
RsaNI	GTAC	7 cut(s)	622, 1042, 1336, 1519, 1589, 1619, 2168
RseI	CAYNNNNRTG	5 cut(s)	675, 1049, 1206, 1614, 2369
SacI	GAGCTC	2 cut(s)	309, 1933
SacII	CCGCGG	1 cut(s)	320
SaqAI	TTAA	7 cut(s)	294, 1320, 1377, 1671, 2289, 2526, 2696
SchI	GAGTC	4 cut(s)	785, 1325, 1501, 1804
SduI	GDGCHC	6 cut(s)	136, 309, 1203, 1347, 1545, 1933
SfaNI	GCATC	8 cut(s)	907, 985, 1135, 1227, 1251, 1372, 1573, 1591
SfcI	CTRYAG	3 cut(s)	383, 1114, 1653
Sfr303I	CCGCGG	1 cut(s)	320
SfuI	TTCGAA	1 cut(s)	527
SgrBI	CCGCGG	1 cut(s)	320
SinI	GGWCC	6 cut(s)	235, 253, 334, 1461, 1559, 2684
SmaI	CCCGGG	3 cut(s)	131, 730, 848
SmiMI	CAYNNNNRTG	5 cut(s)	675, 1049, 1206, 1614, 2369
SmlI	CTYRAG	1 cut(s)	293
SmoI	CTYRAG	1 cut(s)	293
SphI	GCATGC	1 cut(s)	2192
SrfI	GCCCGGGC	1 cut(s)	131
Sse9I	AATT	8 cut(s)	513, 523, 551, 580, 1492, 2225, 2619, 2646
SspI	AATATT	1 cut(s)	2748
SspMI	CTAG	3 cut(s)	720, 1578, 2588
SstI	GAGCTC	2 cut(s)	309, 1933
StyI	CCWWGG	2 cut(s)	1964, 2336
TaaI	ACNGT	2 cut(s)	101, 2595
TaiI	ACGT	5 cut(s)	174, 345, 489, 968, 1996
TaqII	GACCGA	2 cut(s)	415, 2361
TasI	AATT	8 cut(s)	513, 523, 551, 580, 1492, 2225, 2619, 2646
TatI	WGTACW	4 cut(s)	1041, 1518, 1588, 1618
TauI	GCSGC	7 cut(s)	164, 319, 391, 466, 1039, 1075, 2662
TfiI	GAWTC	2 cut(s)	219, 740
Tru1I	TTAA	7 cut(s)	294, 1320, 1377, 1671, 2289, 2526, 2696
Tru9I	TTAA	7 cut(s)	294, 1320, 1377, 1671, 2289, 2526, 2696
TscAI	CASTG	2 cut(s)	2074, 2409
TseFI	GTSAC	4 cut(s)	53, 172, 1051, 1567
TseI	GCWGC	7 cut(s)	382, 385, 460, 878, 1277, 2252, 2420
Tsp45I	GTSAC	4 cut(s)	53, 172, 1051, 1567
TspDTI	ATGAA	7 cut(s)	17, 29, 776, 814, 1413, 1550, 2660
TspMI	CCCGGG	3 cut(s)	129, 728, 846
TspRI	CASTG	2 cut(s)	2074, 2409
Van91I	CCANNNNNTGG	1 cut(s)	2281
Vha464I	CTTAAG	1 cut(s)	293
VneI	GTGCAC	1 cut(s)	1343
VpaK11BI	GGWCC	6 cut(s)	235, 253, 334, 1461, 1559, 2684
XagI	CCTNNNNNAGG	1 cut(s)	720
XapI	RAATTY	1 cut(s)	523
XceI	RCATGY	3 cut(s)	2017, 2192, 2201
XmaI	CCCGGG	3 cut(s)	129, 728, 846
XmiI	GTMKAC	2 cut(s)	483, 2597
XmnI	GAANNNNTTC	1 cut(s)	326
XspI	CTAG	3 cut(s)	720, 1578, 2588
ZraI	GACGTC	2 cut(s)	172, 343

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.