Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0002632

Physical Location & Seq

Genomic Coordinates Forward (+)

9900789 .. 9907155

Gene Structure 6367 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1135800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2805 bp

ATGAAGATGGAAATGGAAAGGCAGATCAGAATCCCCAATCGAAATCCGACGGTCTTCGTTTTCCTTCTACTCCTTGTCAGTTGTCAGCTGTGCTCCGTCGTCTCCTGGAAGAAAGAAGAGTTCAGGAACTGTAACCAAACCCCATTCTGCAAACGAGCCCGTGGCCGCAAACCCAGCTCCTCCTCCTTCGCCGCCCATGACGTATCTATCTCCGACGGTGACCTCACAGCCAGGCTTGTCCCCTCCGACGAAACCCTGGAAGATCAAGACCAAATCAAGCAATTGATTCTCACTCTTTCTGTTTACCAAGATGGGATCTTGCGGCTCAGGATCGACGAGGACCCCAAACTTGATCCACCCAAGAAGCGTTTCGAGGTTCCCGGTGTGGTTATGCCGGAGTTTTTGAGCAAGAAGCTATGGTTGCAGAGGCTTTCAACGGAGACCATCGGCGGCGATGCAAGTCCTTCGTCAATCGTCTACTTGTTGGATGGCTACGAGGCGGTGCTCCGGCACGATCCCTTCGAGGTGTACGTGCGCAAAAAGGGAGGTAATCGCGTAGTTTCTATGAATTCTCATGGTTTATTTGATTTTGAGCAATTGAGGGTGAAAAAGGATGGTGAGGATTGGGAGGAGAGGTTCAAAGGGCATACAGATACGAGGCCTTTCGGCCCGCAATCGATTAGTTTCGATGTGTCCTTTTACGATGCTGATCATGTGTATGGGATTCCTGAGCGTGCTACAAGCTTTGCTCTGAAGCCCACTAGAGGTCCTGGGGTTGAGGTTTCTGAACCCTATAGATTGTTTAACTTAGATGTGTTTGAGTACATTCATGATTCCCCATTTGGACTTTATGGTTCCATACCCTTGATGATTTCCCACGGCAAGTCACGCGGGACTTCTGGTTTCTTTTGGTTGAATGCTGCTGAGATGCAGATTGATGTTCTTGGAACCGGCTGGGATGCAGAGTCTGGCATTTCTCTCCCTACGTCACAATCTAGGATCGATACACATTGGATGAGTGAGGCAGGTATCGTCGATGCCTTCTTTTTCGTAGGACCTGGACCTAAGGATGTAGTCCGCCAGTACACGAGTGTAACTGGAACACCTGCAATGCCACAGTTGTTTGCTGTGGCCTATCACCAATGTAGGTGGAATTATCGTGATGAGGAGGATGTTGAGCAAGTTGATTCTAAGTTTGATGAACACGATATTCCGTATGATGTTTTGTGGCTTGATATTGAGCACACAGATGGGAAGAGATATTTTACATGGGATAGAATGCTTTTTCCACATCCAGAGGAGATGCAGAGGAAGTTGGCTGCTAAAGGTAGGCACATGGTTACTATTGTGGATCCTCATATTAAGCGGGATGATTCCTACTTTTTGCACAAAGAGGCCACAGAAAAGCAGTATTATGTTAGGGATGCCACTGGAAAGGATTATGATGGGTGGTGCTGGTCTGGTTCATCATCGTATTTGGATATGTTGAGGCCAGACATCAGGTCCTGGTGGGCTGAAAAGTTCTCTTATGAGAATTATGTTGGCTCGACTCCTTCACTGTACATCTGGAATGACATGAATGAGCCTTCTGTCTTCAATGGTCCCGAGGTTACAATGCCTAGAGATGCTTTACATGTCGAAGGTGTAGAACATCGAGAGTTACACAATGCCTATGGGTACTACTTTCACATGGCTACAGCAGATGGGCTTGTGAAGCGAGGTGATGGAAGGGATAGACCTTTTGTTTTATCGAGAGCAGTATTTGCTGGAAGTCAAAGGCATGGAGCAATATGGACAGGAGATAATTCAGCTGATTGGGATCACCTTAGGGTTTCAGTTCCAATGGTTTTGACGCTTGGTATTACTGGGATCTCATTCTCAGGTGCTGATGTTGGTGGTTTCTTTGGCAATCCAGAACCTGAGTTGTTAGTTCGTTGGTATCAGTTAGGAGCATACTATCCATTTTTCAGAGCCCATGCCCATCATGACACCAAAAGGCGAGAACCATGGTTATTTGGGGAAAAAAATACTGAACGTATTAGGGAGGCCATACACACTCGTTACATGTTGCTTCCATATTTCTACACATTATTTAGAGAGGCAAACACAACTGGTGTTCCAGTTATTCGTCCATTATGGATGGAGTTCCCTTCTGAAGAAGCTACTTTCAGCAATGATGAGGCTTTCATGATTGGAAGCAGTCTTCTGGTGCAAGGGATTTATACAGAGCGTGCAAGACATGCTTCAGTGTATTTGCCTGGGAAGGAATTATGGTATGACACAAAAACTGGAGTCACATACAAGGGTGGTAAAACCTATAAGTTGGACGTTAATGAAGAAAGTATTCCTGCTTTCCAAAGGGCTGGAACCATCATACCAAGGAAAGACCGGTTTCGTCGAAGTTCCACACAGATGGTGAATGATCCATACACACTGGTGATAGCACTTAATAGTTCACAAGCAGCCGAAGGTGAACTTTACGTAGACGATGGTAGAAGCTTTGGATTTCAGGAAGGGGCCTACATCCATCGTCGTTTTGTATTCTCAGATGGAAAGCTCACATCTCTTAATATGGCACCCGTTGCCCCTGGCCAAAGTCAGTTTTCTTCTGAATGTGTGATCGAGAGGATTATTCTGCAAGGACTCTCCACTGGGCACAAAGGTGCTCTAATTGAACCCGCAAACCAGAAGGCCGAGATTGAACTGGGCCCGCTTCTCCTTCATTCAAAGAAAGGCCCAACTGCAATAACCATCCGCAAGCCGAATGTCCGCGTCGCCGATGATTGGGTGATAAAAGTGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000902 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006491 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009250 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009987 GO:0010035 GO:0010038 GO:0012505 GO:0015926 GO:0016043 GO:0016049 GO:0016051 GO:0016787 GO:0016798 GO:0019538 GO:0030243 GO:0030244 GO:0030587 GO:0031288 GO:0032502 GO:0032989 GO:0033692 GO:0033919 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043687 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0051274 GO:0051703 GO:0051704 GO:0051707 GO:0060560 GO:0071704 GO:0071840 GO:0090599 GO:0090600 GO:0090702 GO:0098542 GO:0099120 GO:1901135 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

935

Amino Acids

106.65

Weight (kDa)

5.93

Isoelectric Point (pI)

42.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	97 - 313	1.5e-39	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	369 - 697	1.6e-120	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	705 - 792	5.9e-25	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0002632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63840 AT5G63840
fragaria_vesca	FvH4_1g25070
malus_domestica	MD01G1007800.v1.1 MD01G1007900.v1.1 MD15G1135800.v1.1
prunus_persica	Prupe.4G277800_v2.0.a1 Prupe.4G277800_v2.0.a1
pyrus_communis	pycom15g12240 pycom520g00330
rosa_chinensis	RchiOBHm_Chr2g0122711 RchiOBHm_Chr2g0122721
rosa_laevigata	RLG00000018707 RLG00000018709
rosa_multiflora	Rmu_sc0005370.1_g000002 Rmu_sc0008259.1_g000009 Rmu_sc0008259.1_g000014 Rmu_sc0017734.1_g000006 Rmu_sc0026486.1_g000001
rosa_roxburghii	Rroxscaffold_1G00031190 Rroxscaffold_2G00121270 Rroxscaffold_2G00121280 Rroxscaffold_4G00297410 Rroxscaffold_4G00317480 Rroxscaffold_4G00325980 Rroxscaffold_7G00197700 Rroxscaffold_7G00199130
rosa_rugosa	Rorug02G0241800 Rorug02G0241800 Rorug02G0241900 Rorug02G0242000 Rorug02G0242100
rosa_samantha	Rh2AG300300 Rh2BG308200 Rh2CG286400 Rh2CG286900 Rh2DG324500
rosa_wichuraiana	Rw2G023850 Rw2G023860 Rw2G024040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1114
Acc16I	TGCGCA	1 cut(s)	536
Acc36I	ACCTGC	2 cut(s)	1016, 1114
AccB1I	GGYRCC	1 cut(s)	2575
AccI	GTMKAC	2 cut(s)	477, 2484
AccII	CGCG	3 cut(s)	555, 891, 2772
AcoI	YGGCCR	2 cut(s)	163, 2590
AcsI	RAATTY	1 cut(s)	568
AcuI	CTGAAG	3 cut(s)	773, 2175, 2229
AfaI	GTAC	5 cut(s)	530, 824, 1085, 1562, 1679
AfiI	CCNNNNNNNGG	2 cut(s)	764, 2784
AflIII	ACRYGT	2 cut(s)	1633, 2064
AgeI	ACCGGT	1 cut(s)	2388
AgsI	TTSAA	7 cut(s)	435, 640, 916, 1597, 2675, 2702, 2727
AhdI	GACNNNNNGTC	1 cut(s)	1501
AjnI	CCWGG	8 cut(s)	104, 230, 255, 769, 1057, 1505, 2257, 2587
AjuI	GAANNNNNNNTTGG	2 cut(s)	353, 385
AleI	CACNNNNGTG	2 cut(s)	2435, 2661
AloI	GAACNNNNNNTCC	2 cut(s)	620, 652
AluBI	AGCT	8 cut(s)	88, 177, 415, 744, 1811, 2162, 2499, 2557
AluI	AGCT	8 cut(s)	88, 177, 415, 744, 1811, 2162, 2499, 2557
Alw21I	GWGCWC	4 cut(s)	95, 507, 1245, 2668
Alw26I	GTCTC	2 cut(s)	106, 434
AlwNI	CAGNNNCTG	5 cut(s)	129, 968, 1506, 1886, 1919
Ama87I	CYCGRG	1 cut(s)	1604
ApaI	GGGCCC	1 cut(s)	2711
ApeKI	GCWGC	3 cut(s)	920, 1319, 2462
ApoI	RAATTY	1 cut(s)	568
AsiGI	ACCGGT	1 cut(s)	2388
Asp700I	GAANNNNTTC	2 cut(s)	368, 2343
AspLEI	GCGC	1 cut(s)	537
AsuC2I	CCSGG	1 cut(s)	381
AvaI	CYCGRG	1 cut(s)	1604
AvaII	GGWCC	6 cut(s)	340, 767, 1055, 1061, 1503, 1601
AxyI	CCTNAGG	2 cut(s)	1065, 1826
BaeGI	GKGCMC	2 cut(s)	2658, 2711
BalI	TGGCCA	1 cut(s)	2592
BamHI	GGATCC	1 cut(s)	1351
BanI	GGYRCC	1 cut(s)	2575
BanII	GRGCYC	3 cut(s)	160, 1975, 2711
BauI	CACGAG	1 cut(s)	1087
BbsI	GAAGAC	3 cut(s)	46, 1585, 2195
Bbv12I	GWGCWC	4 cut(s)	95, 507, 1245, 2668
BbvI	GCAGC	3 cut(s)	907, 1306, 2474
BceAI	ACGGC	1 cut(s)	895
BcgI	CGANNNNNNTGC	2 cut(s)	447, 481
BciT130I	CCWGG	8 cut(s)	106, 232, 257, 771, 1059, 1507, 2259, 2589
BclI	TGATCA	1 cut(s)	709
BcnI	CCSGG	1 cut(s)	381
BcoDI	GTCTC	2 cut(s)	106, 434
BfaI	CTAG	3 cut(s)	762, 996, 1620
BfmI	CTRYAG	2 cut(s)	793, 1695
BfuAI	ACCTGC	2 cut(s)	1016, 1114
BisI	GCNGC	7 cut(s)	166, 192, 323, 451, 921, 1320, 2463
BlsI	GCNGC	7 cut(s)	167, 193, 324, 452, 922, 1321, 2464
Bme1390I	CCNGG	9 cut(s)	106, 232, 257, 381, 771, 1059, 1507, 2259, 2589
Bme18I	GGWCC	6 cut(s)	340, 767, 1055, 1061, 1503, 1601
BmeRI	GACNNNNNGTC	1 cut(s)	1501
BmeT110I	CYCGRG	1 cut(s)	1604
BmrFI	CCNGG	9 cut(s)	106, 232, 257, 381, 771, 1059, 1507, 2259, 2589
BmrI	ACTGGG	3 cut(s)	1875, 2661, 2714
BmsI	GCATC	8 cut(s)	445, 694, 918, 949, 1027, 1293, 1414, 1615
BmuI	ACTGGG	3 cut(s)	1875, 2661, 2714
BpiI	GAAGAC	3 cut(s)	46, 1585, 2195
BpmI	CTGGAG	1 cut(s)	2310
Bpu10I	CCTNAGC	2 cut(s)	326, 729
BpuMI	CCSGG	1 cut(s)	381
Bsa29I	ATCGAT	2 cut(s)	677, 1002
BsaAI	YACGTR	2 cut(s)	532, 2482
BsaBI	GATNNNNATC	3 cut(s)	29, 708, 1818
BsaI	GGTCTC	1 cut(s)	434
BsaJI	CCNNGG	9 cut(s)	160, 255, 770, 877, 1605, 2006, 2258, 2378, 2587
BsaWI	WCCGGW	1 cut(s)	2388
BsaXI	ACNNNNNCTCC	2 cut(s)	620, 650
Bsc4I	CCNNNNNNNGG	2 cut(s)	764, 2784
Bse118I	RCCGGY	2 cut(s)	950, 2388
Bse21I	CCTNAGG	2 cut(s)	1065, 1826
Bse3DI	GCAATG	2 cut(s)	1116, 2179
Bse8I	GATNNNNATC	3 cut(s)	29, 708, 1818
BseBI	CCWGG	8 cut(s)	106, 232, 257, 771, 1059, 1507, 2259, 2589
BseCI	ATCGAT	2 cut(s)	677, 1002
BseDI	CCNNGG	9 cut(s)	160, 255, 770, 877, 1605, 2006, 2258, 2378, 2587
BseJI	GATNNNNATC	3 cut(s)	29, 708, 1818
BseLI	CCNNNNNNNGG	2 cut(s)	764, 2784
BseMI	GCAATG	2 cut(s)	1116, 2179
BseMII	CTCAG	6 cut(s)	340, 720, 915, 1893, 1911, 2559
BseRI	GAGGAG	5 cut(s)	169, 172, 644, 1181, 1313
BseSI	GKGCMC	2 cut(s)	2658, 2711
BseXI	GCAGC	3 cut(s)	907, 1306, 2474
BseYI	CCCAGC	2 cut(s)	173, 954
Bsh1236I	CGCG	3 cut(s)	555, 891, 2772
BshNI	GGYRCC	1 cut(s)	2575
BshTI	ACCGGT	1 cut(s)	2388
BshVI	ATCGAT	2 cut(s)	677, 1002
BsiHKAI	GWGCWC	4 cut(s)	95, 507, 1245, 2668
BsiHKCI	CYCGRG	1 cut(s)	1604
BsiSI	CCGG	5 cut(s)	381, 395, 508, 951, 2389
BslFI	GGGAC	3 cut(s)	224, 907, 1587
BslI	CCNNNNNNNGG	2 cut(s)	764, 2784
BsmAI	GTCTC	2 cut(s)	106, 434
BsmBI	CGTCTC	1 cut(s)	106
BsmFI	GGGAC	3 cut(s)	224, 907, 1587
BsmI	GAATGC	2 cut(s)	922, 1284
Bso31I	GGTCTC	1 cut(s)	434
BsoBI	CYCGRG	1 cut(s)	1604
Bsp120I	GGGCCC	1 cut(s)	2707
Bsp1286I	GDGCHC	8 cut(s)	95, 160, 507, 1245, 1975, 2658, 2668, 2711
Bsp1407I	TGTACA	1 cut(s)	1560
Bsp19I	CCATGG	1 cut(s)	2006
BspCNI	CTCAG	6 cut(s)	339, 721, 916, 1892, 1912, 2558
BspDI	ATCGAT	2 cut(s)	677, 1002
BspFNI	CGCG	3 cut(s)	555, 891, 2772
BspHI	TCATGA	3 cut(s)	829, 1984, 2187
BspMI	ACCTGC	2 cut(s)	1016, 1114
BspT107I	GGYRCC	1 cut(s)	2575
BspTNI	GGTCTC	1 cut(s)	434
BsrDI	GCAATG	2 cut(s)	1116, 2179
BsrFI	RCCGGY	2 cut(s)	950, 2388
BsrGI	TGTACA	1 cut(s)	1560
BssAI	RCCGGY	2 cut(s)	950, 2388
BssECI	CCNNGG	9 cut(s)	160, 255, 770, 877, 1605, 2006, 2258, 2378, 2587
BssSI	CACGAG	1 cut(s)	1087
BssT1I	CCWWGG	2 cut(s)	2006, 2378
Bst2BI	CACGAG	1 cut(s)	1087
Bst2UI	CCWGG	8 cut(s)	106, 232, 257, 771, 1059, 1507, 2259, 2589
Bst4CI	ACNGT	5 cut(s)	52, 131, 218, 1119, 1560
Bst6I	CTCTTC	2 cut(s)	111, 1250
BstAPI	GCANNNNNTGC	3 cut(s)	1763, 2240, 2582
BstAUI	TGTACA	1 cut(s)	1560
BstBAI	YACGTR	2 cut(s)	532, 2482
BstC8I	GCNNGC	5 cut(s)	671, 735, 2232, 2711, 2759
BstDSI	CCRYGG	3 cut(s)	160, 877, 2006
BstEII	GGTNACC	1 cut(s)	218
BstFNI	CGCG	3 cut(s)	555, 891, 2772
BstHHI	GCGC	1 cut(s)	537
BstMAI	GTCTC	2 cut(s)	106, 434
BstMWI	GCNNNNNNNGC	7 cut(s)	174, 421, 888, 1714, 1763, 2240, 2582
BstNI	CCWGG	8 cut(s)	106, 232, 257, 771, 1059, 1507, 2259, 2589
BstNSI	RCATGY	3 cut(s)	1637, 2068, 2243
BstPI	GGTNACC	1 cut(s)	218
BstSCI	CCNGG	9 cut(s)	104, 230, 255, 379, 769, 1057, 1505, 2257, 2587
BstSFI	CTRYAG	2 cut(s)	793, 1695
BstSLI	GKGCMC	2 cut(s)	2658, 2711
BstSNI	TACGTA	1 cut(s)	2482
BstUI	CGCG	3 cut(s)	555, 891, 2772
BstV1I	GCAGC	3 cut(s)	907, 1306, 2474
BstV2I	GAAGAC	3 cut(s)	46, 1585, 2195
BstX2I	RGATCY	3 cut(s)	315, 1351, 1869
BstXI	CCANNNNNNTGG	2 cut(s)	2363, 2413
BstYI	RGATCY	3 cut(s)	315, 1351, 1869
Bsu15I	ATCGAT	2 cut(s)	677, 1002
Bsu36I	CCTNAGG	2 cut(s)	1065, 1826
BsuTUI	ATCGAT	2 cut(s)	677, 1002
BtgI	CCRYGG	3 cut(s)	160, 877, 2006
BtgZI	GCGATG	1 cut(s)	468
BtsIMutI	CAGTG	5 cut(s)	1428, 1556, 2253, 2432, 2649
BveI	ACCTGC	2 cut(s)	1016, 1114
Cac8I	GCNNGC	5 cut(s)	671, 735, 2232, 2711, 2759
CaiI	CAGNNNCTG	5 cut(s)	129, 968, 1506, 1886, 1919
CciI	TCATGA	3 cut(s)	829, 1984, 2187
CfoI	GCGC	1 cut(s)	537
Cfr10I	RCCGGY	2 cut(s)	950, 2388
ClaI	ATCGAT	2 cut(s)	677, 1002
CseI	GACGC	2 cut(s)	1861, 2761
Csp6I	GTAC	5 cut(s)	529, 823, 1084, 1561, 1678
CspAI	ACCGGT	1 cut(s)	2388
CspCI	CAANNNNNGTGG	2 cut(s)	1130, 1165
CviQI	GTAC	5 cut(s)	529, 823, 1084, 1561, 1678
DriI	GACNNNNNGTC	1 cut(s)	1501
EaeI	YGGCCR	2 cut(s)	163, 2590
Eam1104I	CTCTTC	2 cut(s)	111, 1250
Eam1105I	GACNNNNNGTC	1 cut(s)	1501
EarI	CTCTTC	2 cut(s)	111, 1250
EciI	GGCGGA	1 cut(s)	1067
Eco105I	TACGTA	1 cut(s)	2482
Eco130I	CCWWGG	2 cut(s)	2006, 2378
Eco147I	AGGCCT	1 cut(s)	661
Eco24I	GRGCYC	3 cut(s)	160, 1975, 2711
Eco31I	GGTCTC	1 cut(s)	434
Eco47I	GGWCC	6 cut(s)	340, 767, 1055, 1061, 1503, 1601
Eco57I	CTGAAG	3 cut(s)	773, 2175, 2229
Eco81I	CCTNAGG	2 cut(s)	1065, 1826
Eco88I	CYCGRG	1 cut(s)	1604
Eco91I	GGTNACC	1 cut(s)	218
EcoO109I	RGGNCCY	5 cut(s)	340, 767, 1055, 1503, 2517
EcoO65I	GGTNACC	1 cut(s)	218
EcoRI	GAATTC	1 cut(s)	568
EcoRII	CCWGG	8 cut(s)	104, 230, 255, 769, 1057, 1505, 2257, 2587
EcoT14I	CCWWGG	2 cut(s)	2006, 2378
EcoT38I	GRGCYC	3 cut(s)	160, 1975, 2711
ErhI	CCWWGG	2 cut(s)	2006, 2378
Esp3I	CGTCTC	1 cut(s)	106
FalI	AAGNNNNNCTT	4 cut(s)	2227, 2259, 2460, 2492
FaqI	GGGAC	3 cut(s)	224, 907, 1587
FauI	CCCGC	5 cut(s)	678, 884, 1359, 2686, 2718
FbaI	TGATCA	1 cut(s)	709
FblI	GTMKAC	2 cut(s)	477, 2484
Fnu4HI	GCNGC	7 cut(s)	166, 192, 323, 451, 921, 1320, 2463
FriOI	GRGCYC	3 cut(s)	160, 1975, 2711
Fsp4HI	GCNGC	7 cut(s)	166, 192, 323, 451, 921, 1320, 2463
FspBI	CTAG	3 cut(s)	762, 996, 1620
FspI	TGCGCA	1 cut(s)	536
GlaI	GCGC	1 cut(s)	536
GluI	GCNGC	7 cut(s)	166, 192, 323, 451, 921, 1320, 2463
GsaI	CCCAGC	2 cut(s)	177, 958
GsuI	CTGGAG	1 cut(s)	2310
HapII	CCGG	5 cut(s)	381, 395, 508, 951, 2389
HgaI	GACGC	2 cut(s)	1861, 2761
HhaI	GCGC	1 cut(s)	537
Hin6I	GCGC	1 cut(s)	535
HinP1I	GCGC	1 cut(s)	535
HindIII	AAGCTT	2 cut(s)	742, 2497
HpaII	CCGG	5 cut(s)	381, 395, 508, 951, 2389
Hpy166II	GTNNAC	7 cut(s)	304, 478, 529, 1086, 2456, 2474, 2485
Hpy8I	GTNNAC	7 cut(s)	304, 478, 529, 1086, 2456, 2474, 2485
Hpy99I	CGWCG	9 cut(s)	52, 101, 218, 251, 338, 1037, 2400, 2535, 2777
HpyCH4III	ACNGT	5 cut(s)	52, 131, 218, 1119, 1560
HpyCH4IV	ACGT	6 cut(s)	201, 531, 986, 2035, 2328, 2481
HpyF10VI	GCNNNNNNNGC	7 cut(s)	174, 421, 888, 1714, 1763, 2240, 2582
HpySE526I	ACGT	6 cut(s)	201, 531, 986, 2035, 2328, 2481
HspAI	GCGC	1 cut(s)	535
Ksp22I	TGATCA	1 cut(s)	709
LmnI	GCTCC	5 cut(s)	98, 182, 510, 1784, 1949
Lsp1109I	GCAGC	3 cut(s)	907, 1306, 2474
LweI	GCATC	8 cut(s)	445, 694, 918, 949, 1027, 1293, 1414, 1615
MaeI	CTAG	3 cut(s)	762, 996, 1620
MaeII	ACGT	6 cut(s)	201, 531, 986, 2035, 2328, 2481
MfeI	CAATTG	2 cut(s)	281, 596
MflI	RGATCY	3 cut(s)	315, 1351, 1869
MhlI	GDGCHC	8 cut(s)	95, 160, 507, 1245, 1975, 2658, 2668, 2711
MlsI	TGGCCA	1 cut(s)	2592
MluCI	AATT	8 cut(s)	281, 568, 596, 1153, 1534, 1804, 2267, 2670
MluNI	TGGCCA	1 cut(s)	2592
MlyI	GAGTC	4 cut(s)	974, 1543, 2301, 2637
MmeI	TCCRAC	5 cut(s)	71, 237, 270, 465, 2304
Mox20I	TGGCCA	1 cut(s)	2592
MroXI	GAANNNNTTC	2 cut(s)	368, 2343
MscI	TGGCCA	1 cut(s)	2592
MseI	TTAA	5 cut(s)	804, 1362, 2331, 2448, 2568
MslI	CAYNNNNRTG	5 cut(s)	717, 1248, 2411, 2435, 2661
Msp20I	TGGCCA	1 cut(s)	2592
MspA1I	CMGCKG	2 cut(s)	88, 1811
MspI	CCGG	5 cut(s)	381, 395, 508, 951, 2389
MspR9I	CCNGG	9 cut(s)	106, 232, 257, 381, 771, 1059, 1507, 2259, 2589
MunI	CAATTG	2 cut(s)	281, 596
Mva1269I	GAATGC	2 cut(s)	922, 1284
MvaI	CCWGG	8 cut(s)	106, 232, 257, 771, 1059, 1507, 2259, 2589
MvnI	CGCG	3 cut(s)	555, 891, 2772
MwoI	GCNNNNNNNGC	7 cut(s)	174, 421, 888, 1714, 1763, 2240, 2582
NciI	CCSGG	1 cut(s)	381
NcoI	CCATGG	1 cut(s)	2006
NmeAIII	GCCGAG	1 cut(s)	2719
NmuCI	GTSAC	4 cut(s)	218, 885, 987, 2293
NsbI	TGCGCA	1 cut(s)	536
NspI	RCATGY	3 cut(s)	1637, 2068, 2243
OliI	CACNNNNGTG	2 cut(s)	2435, 2661
PagI	TCATGA	3 cut(s)	829, 1984, 2187
PaqCI	CACCTGC	1 cut(s)	1114
PceI	AGGCCT	1 cut(s)	661
PciI	ACATGT	2 cut(s)	1633, 2064
PcsI	WCGNNNNNNNCGW	2 cut(s)	519, 528
PctI	GAATGC	2 cut(s)	922, 1284
PdmI	GAANNNNTTC	2 cut(s)	368, 2343
PfeI	GAWTC	6 cut(s)	30, 286, 724, 833, 1187, 1373
PfoI	TCCNGGA	1 cut(s)	104
PinAI	ACCGGT	1 cut(s)	2388
PkrI	GCNGC	7 cut(s)	167, 193, 324, 452, 922, 1321, 2464
PleI	GAGTC	4 cut(s)	973, 1543, 2300, 2637
PpsI	GAGTC	4 cut(s)	973, 1543, 2300, 2637
Ppu21I	YACGTR	2 cut(s)	532, 2482
PpuMI	RGGWCCY	4 cut(s)	340, 767, 1055, 1503
PscI	ACATGT	2 cut(s)	1633, 2064
Psp5II	RGGWCCY	4 cut(s)	340, 767, 1055, 1503
Psp6I	CCWGG	8 cut(s)	104, 230, 255, 769, 1057, 1505, 2257, 2587
PspEI	GGTNACC	1 cut(s)	218
PspFI	CCCAGC	2 cut(s)	173, 954
PspGI	CCWGG	8 cut(s)	104, 230, 255, 769, 1057, 1505, 2257, 2587
PspOMI	GGGCCC	1 cut(s)	2707
PspPPI	RGGWCCY	4 cut(s)	340, 767, 1055, 1503
PstNI	CAGNNNCTG	5 cut(s)	129, 968, 1506, 1886, 1919
PsuI	RGATCY	3 cut(s)	315, 1351, 1869
PvuII	CAGCTG	2 cut(s)	88, 1811
RsaI	GTAC	5 cut(s)	530, 824, 1085, 1562, 1679
RsaNI	GTAC	5 cut(s)	529, 823, 1084, 1561, 1678
RseI	CAYNNNNRTG	5 cut(s)	717, 1248, 2411, 2435, 2661
SaqAI	TTAA	5 cut(s)	804, 1362, 2331, 2448, 2568
SatI	GCNGC	7 cut(s)	166, 192, 323, 451, 921, 1320, 2463
SchI	GAGTC	4 cut(s)	974, 1543, 2301, 2637
ScrFI	CCNGG	9 cut(s)	106, 232, 257, 381, 771, 1059, 1507, 2259, 2589
SduI	GDGCHC	8 cut(s)	95, 160, 507, 1245, 1975, 2658, 2668, 2711
SfaNI	GCATC	8 cut(s)	445, 694, 918, 949, 1027, 1293, 1414, 1615
SfcI	CTRYAG	2 cut(s)	793, 1695
SinI	GGWCC	6 cut(s)	340, 767, 1055, 1061, 1503, 1601
SmiMI	CAYNNNNRTG	5 cut(s)	717, 1248, 2411, 2435, 2661
SnaBI	TACGTA	1 cut(s)	2482
Sse9I	AATT	8 cut(s)	281, 568, 596, 1153, 1534, 1804, 2267, 2670
SseBI	AGGCCT	1 cut(s)	661
SspMI	CTAG	3 cut(s)	762, 996, 1620
StuI	AGGCCT	1 cut(s)	661
StyD4I	CCNGG	9 cut(s)	104, 230, 255, 379, 769, 1057, 1505, 2257, 2587
StyI	CCWWGG	2 cut(s)	2006, 2378
TaaI	ACNGT	5 cut(s)	52, 131, 218, 1119, 1560
TaiI	ACGT	6 cut(s)	204, 534, 989, 2038, 2331, 2484
TasI	AATT	8 cut(s)	281, 568, 596, 1153, 1534, 1804, 2267, 2670
TatI	WGTACW	3 cut(s)	822, 1083, 1560
TauI	GCSGC	4 cut(s)	168, 194, 325, 453
TfiI	GAWTC	6 cut(s)	30, 286, 724, 833, 1187, 1373
Tru1I	TTAA	5 cut(s)	804, 1362, 2331, 2448, 2568
Tru9I	TTAA	5 cut(s)	804, 1362, 2331, 2448, 2568
TscAI	CASTG	5 cut(s)	1435, 1563, 2253, 2439, 2656
TseFI	GTSAC	4 cut(s)	218, 885, 987, 2293
TseI	GCWGC	3 cut(s)	920, 1319, 2462
Tsp45I	GTSAC	4 cut(s)	218, 885, 987, 2293
TspDTI	ATGAA	9 cut(s)	17, 581, 818, 1215, 1455, 1592, 2176, 2349, 2711
TspGWI	ACGGA	3 cut(s)	85, 452, 1203
TspRI	CASTG	5 cut(s)	1435, 1563, 2253, 2439, 2656
VpaK11BI	GGWCC	6 cut(s)	340, 767, 1055, 1061, 1503, 1601
XapI	RAATTY	1 cut(s)	568
XceI	RCATGY	3 cut(s)	1637, 2068, 2243
XmiI	GTMKAC	2 cut(s)	477, 2484
XmnI	GAANNNNTTC	2 cut(s)	368, 2343
XspI	CTAG	3 cut(s)	762, 996, 1620

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.