Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2D

Orthogroup ID

OG0002632

Physical Location & Seq

Genomic Coordinates Reverse (-)

40801744 .. 40808527

Gene Structure 6784 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2DG324500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2004 bp

ATGAAGAAGACCCAGACGATGAACAACCTCACTCTTCTCCTTCTCCTCCTCCTCCTTCTCACCTGTCACCTCTCCCCAGTTCTCTCATGGAAAAAAGACGAGTTCCGAAACTGTAACCAAACCCCTTTCTGCAAACGAGCCCGAGCTCGTTACCCCGGCTCGTCCTCGCTCACAGCCCAAGACGCCGCCATCTCCGACGGCGAGCTCACCGCCAAGCTCGTCCCCAAGAGAATCGAACAAGAAGAAACAACCCCCGATCAAATCAGGCCCTTGCTTCTCACTCTCTCTGCTTACCAAGACGGGATCTTGCGCCTCAGGATCGACGAGCTCGATCCGCCCCGGAAGCGCTTCGAGGTCCCCGACGTCGTTTTGCCGGAGTTCGAGGGCAAGAAGCTCTGGCTGCAGAGGCTTTCGACCGAGACGATCGACGGCGACACGGCTCCGTCGTCGGTCGTGTTCTTGTCCGACGGCTACGAGGCGGTGCTGCGGCACGATCCGTTCGAGGTTTACGTCCGCGAGAAGAGGGGTAATCGTGTAATTTCCGTGAATTCCAATGGCTTATTTGATTTTGAGCAGCTGAGAGAGAAGAAGGGAGAGGGTGAGGATTGGGAAGAGAGGTTTAGAGGGCACACGGATAGGAGGCCCTACGGCCCTCAGTCCATTAGTTTTGATGTGTCGTTTTATGGGGCTGATCATGTTTACGGGATCCCTGAGCGTGCTTCAAGCTTGGCTTTGAAGCCCACTAGAGGCCCTGGGGTTGAGGAATCTGAACCCTATAGACTGTTTAACTTGGACGTGTTTGAGTATATTCATGATTCCCCATTTGGGCTTTATGGCTCGATTCCATTCATGATTTCCCACGGTAAGGCCCGAGGGACTTCCGGGTTCTTTTGGTTGAATGCTGCTGAAATGCAGATTGATGTTCTTGGGAGCGGGTGGGATGCTGAGGCCGGCATTGCTCTCCCTTCGTCGCAGAGTAGGATTGACACGTTTTGGATGAGTGAGGCCGGTATTGTGGATGCTTTCTTTTTCGTGGGACCGGGGCCAAAGGATGTAGCTCGTCAGTATGCGAGTGTGACTGGCACGCCCGCAATGCCGCAGCTGTTTGCGGTGGCGTATCACCAATGTAGGTGGAATTATAGAGATGAGGAGGATGTGGAGCAAGTTGATTCTAAGTTTGATGAGCATGATATTCCGTATGATGTTTTGTGGCTTGATATTGAGCACACAGACGGGAAGAGGTATTTTACATGGGATAGGATGCTTTTCCCGCACCCGGAGGAGATGCAGAGGAAGTTGGCTGCTAAGGGTAGGCACATGGTTACTATTGTGGACCCTCATATTAAGCGAGATGACTCGTACTTTTTGCACAGGGAGGCCACAGAAAAAGGCTATTATGTTAAGGATGCCAATGGGAATGATTACGATGGGTGGTGCTGGTCTGGTTCGTCATCATATTTGGATATGTTGAGGCCAGAGGTTAGGTCCTGGTGGGCTACAAAGTTCTCTACTGAAAATTATGTTGGTTCAACTCCCTCCTTGTACATCTGGAATGACATGAATGAGCCTTCTGTCTTCAATGGTCCTGAGGTCACAATGCCTAGAGATGCTGTACATCTAGGAGATGTAGAACACCGGGAGTTACATAACGCCTATGGTTACTACTTTCACATGGCTACAGCAGATGGGCTTGTTCAGCGAGGTGATGGAAGGGATAGGCCATTTGTTTTATCGAGAGCAGTATTTGCTGGAAGTCAAAGGCATGGAGCAGTATGGACGGGAGACAATACAGCTGAATGGGATCACCTCAGGGTTTCAGTTCCAATGATTTTGACTCTTGGTCTTACTGGGATATCATTCTCTGGTGCTGATGTGGGTGGGTATTTTGGCAATCCAGAACCTGAGTTGTGGGTTCGTTGGTATCAATTAGGAGCCTACTATCCTTTCTTTAGAGGTCATGCTCACCATGACACCAAAAGACGAGAACCATGGTTATTTGGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000902 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006491 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009250 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009987 GO:0010035 GO:0010038 GO:0012505 GO:0015926 GO:0016043 GO:0016049 GO:0016051 GO:0016787 GO:0016798 GO:0019538 GO:0030243 GO:0030244 GO:0030587 GO:0031288 GO:0032502 GO:0032989 GO:0033692 GO:0033919 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043687 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0051274 GO:0051703 GO:0051704 GO:0051707 GO:0060560 GO:0071704 GO:0071840 GO:0090599 GO:0090600 GO:0090702 GO:0098542 GO:0099120 GO:1901135 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

667

Amino Acids

76.36

Weight (kDa)

5.45

Isoelectric Point (pI)

42.56

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	93 - 307	5.7e-41	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	363 - 667	1e-108	Glycosyl hydrolases family 31 TIM-barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63840 AT5G63840
fragaria_vesca	FvH4_1g25070
malus_domestica	MD01G1007800.v1.1 MD01G1007900.v1.1 MD15G1135800.v1.1
prunus_persica	Prupe.4G277800_v2.0.a1 Prupe.4G277800_v2.0.a1
pyrus_communis	pycom15g12240 pycom520g00330
rosa_chinensis	RchiOBHm_Chr2g0122711 RchiOBHm_Chr2g0122721
rosa_laevigata	RLG00000018707 RLG00000018709
rosa_multiflora	Rmu_sc0005370.1_g000002 Rmu_sc0008259.1_g000009 Rmu_sc0008259.1_g000014 Rmu_sc0017734.1_g000006 Rmu_sc0026486.1_g000001
rosa_roxburghii	Rroxscaffold_1G00031190 Rroxscaffold_2G00121270 Rroxscaffold_2G00121280 Rroxscaffold_4G00297410 Rroxscaffold_4G00317480 Rroxscaffold_4G00325980 Rroxscaffold_7G00197700 Rroxscaffold_7G00199130
rosa_rugosa	Rorug02G0241800 Rorug02G0241800 Rorug02G0241900 Rorug02G0242000 Rorug02G0242100
rosa_samantha	Rh2AG300300 Rh2BG308200 Rh2CG286400 Rh2CG286900 Rh2DG324500
rosa_wichuraiana	Rw2G023850 Rw2G023860 Rw2G024040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	366
AccBSI	CCGCTC	1 cut(s)	933
AccII	CGCG	1 cut(s)	516
AclWI	GGATC	7 cut(s)	311, 326, 326, 488, 700, 713, 1809
AcsI	RAATTY	1 cut(s)	547
AcyI	GRCGYC	2 cut(s)	183, 363
AfaI	GTAC	3 cut(s)	1361, 1544, 1614
AfeI	AGCGCT	1 cut(s)	347
AfiI	CCNNNNNNNGG	4 cut(s)	746, 825, 865, 1276
AflIII	ACRYGT	2 cut(s)	795, 987
AgsI	TTSAA	5 cut(s)	723, 736, 898, 1530, 1579
AhdI	GACNNNNNGTC	1 cut(s)	1839
AjiI	CACGTC	1 cut(s)	796
AjnI	CCWGG	2 cut(s)	751, 1487
AjuI	GAANNNNNNNTTGG	2 cut(s)	1506, 1538
Alw21I	GWGCWC	4 cut(s)	148, 207, 330, 1227
Alw26I	GTCTC	2 cut(s)	413, 1776
AlwI	GGATC	7 cut(s)	311, 326, 326, 488, 700, 713, 1809
AlwNI	CAGNNNCTG	1 cut(s)	1901
Ama87I	CYCGRG	2 cut(s)	141, 870
Aor51HI	AGCGCT	1 cut(s)	347
ApeKI	GCWGC	6 cut(s)	400, 484, 574, 902, 1099, 1301
ApoI	RAATTY	1 cut(s)	547
Asp700I	GAANNNNTTC	1 cut(s)	347
AspLEI	GCGC	2 cut(s)	312, 348
AsuC2I	CCSGG	6 cut(s)	156, 340, 883, 1041, 1277, 1637
AsuHPI	GGTGA	8 cut(s)	52, 59, 199, 611, 1112, 1715, 1796, 1955
AvaI	CYCGRG	2 cut(s)	141, 870
AvaII	GGWCC	5 cut(s)	355, 1037, 1333, 1485, 1583
AxyI	CCTNAGG	3 cut(s)	314, 1587, 1808
BaeGI	GKGCMC	1 cut(s)	630
BamHI	GGATCC	1 cut(s)	705
BanII	GRGCYC	4 cut(s)	142, 148, 207, 330
BbsI	GAAGAC	2 cut(s)	14, 1567
Bbv12I	GWGCWC	4 cut(s)	148, 207, 330, 1227
BbvCI	CCTCAGC	1 cut(s)	945
BbvI	GCAGC	6 cut(s)	387, 471, 586, 889, 1111, 1288
BccI	CCATC	4 cut(s)	197, 1421, 1679, 1700
BceAI	ACGGC	5 cut(s)	214, 445, 453, 484, 664
BciT130I	CCWGG	2 cut(s)	753, 1489
BclI	TGATCA	1 cut(s)	691
BcnI	CCSGG	6 cut(s)	156, 340, 883, 1041, 1277, 1637
BcoDI	GTCTC	2 cut(s)	413, 1776
BfaI	CTAG	3 cut(s)	744, 1602, 1619
BfmI	CTRYAG	3 cut(s)	401, 775, 1677
BfoI	RGCGCY	1 cut(s)	349
BisI	GCNGC	9 cut(s)	186, 401, 485, 488, 575, 903, 1097, 1100, 1302
BlsI	GCNGC	9 cut(s)	187, 402, 486, 489, 576, 904, 1098, 1101, 1303
Bme1390I	CCNGG	8 cut(s)	156, 340, 753, 883, 1041, 1277, 1489, 1637
Bme18I	GGWCC	5 cut(s)	355, 1037, 1333, 1485, 1583
BmeRI	GACNNNNNGTC	1 cut(s)	1839
BmeT110I	CYCGRG	2 cut(s)	141, 870
BmgBI	CACGTC	1 cut(s)	796
BmiI	GGNNCC	7 cut(s)	357, 441, 707, 1038, 1044, 1335, 1933
BmrFI	CCNGG	8 cut(s)	156, 340, 753, 883, 1041, 1277, 1489, 1637
BmrI	ACTGGG	2 cut(s)	71, 1857
BmsI	GCATC	6 cut(s)	931, 1009, 1251, 1275, 1396, 1597
BmuI	ACTGGG	2 cut(s)	71, 1857
BpiI	GAAGAC	2 cut(s)	14, 1567
Bpu10I	CCTNAGC	3 cut(s)	711, 945, 1305
BpuMI	CCSGG	6 cut(s)	156, 340, 883, 1041, 1277, 1637
BsaHI	GRCGYC	2 cut(s)	183, 363
BsaJI	CCNNGG	8 cut(s)	154, 338, 751, 752, 859, 871, 1040, 1988
Bsc4I	CCNNNNNNNGG	4 cut(s)	746, 825, 865, 1276
Bse118I	RCCGGY	2 cut(s)	950, 1007
Bse1I	ACTGG	3 cut(s)	77, 1084, 1852
Bse21I	CCTNAGG	3 cut(s)	314, 1587, 1808
Bse3DI	GCAATG	2 cut(s)	954, 1098
BseBI	CCWGG	2 cut(s)	753, 1489
BseDI	CCNNGG	8 cut(s)	154, 338, 751, 752, 859, 871, 1040, 1988
BseGI	GGATG	7 cut(s)	946, 1002, 1024, 1057, 1159, 1266, 1411
BseLI	CCNNNNNNNGG	4 cut(s)	746, 825, 865, 1276
BseMI	GCAATG	2 cut(s)	954, 1098
BseMII	CTCAG	8 cut(s)	328, 569, 668, 702, 936, 1578, 1822, 1893
BseNI	ACTGG	3 cut(s)	77, 1084, 1852
BseRI	GAGGAG	5 cut(s)	35, 38, 41, 1163, 1295
BseSI	GKGCMC	1 cut(s)	630
BseXI	GCAGC	6 cut(s)	387, 471, 586, 889, 1111, 1288
Bsh1236I	CGCG	1 cut(s)	516
Bsh1285I	CGRYCG	3 cut(s)	417, 426, 453
BsiEI	CGRYCG	3 cut(s)	417, 426, 453
BsiHKAI	GWGCWC	4 cut(s)	148, 207, 330, 1227
BsiHKCI	CYCGRG	2 cut(s)	141, 870
BsiSI	CCGG	9 cut(s)	156, 340, 374, 882, 951, 1008, 1040, 1277, 1636
BslFI	GGGAC	4 cut(s)	206, 341, 889, 1050
BslI	CCNNNNNNNGG	4 cut(s)	746, 825, 865, 1276
BsmAI	GTCTC	2 cut(s)	413, 1776
BsmBI	CGTCTC	1 cut(s)	413
BsmFI	GGGAC	4 cut(s)	206, 341, 889, 1050
BsmI	GAATGC	1 cut(s)	904
BsoBI	CYCGRG	2 cut(s)	141, 870
Bsp1286I	GDGCHC	6 cut(s)	142, 148, 207, 330, 630, 1227
Bsp1407I	TGTACA	2 cut(s)	1542, 1612
Bsp143I	GATC	9 cut(s)	256, 303, 318, 331, 423, 493, 691, 705, 1801
Bsp19I	CCATGG	1 cut(s)	1988
BspCNI	CTCAG	8 cut(s)	327, 570, 667, 703, 937, 1579, 1821, 1894
BspFNI	CGCG	1 cut(s)	516
BspHI	TCATGA	2 cut(s)	811, 849
BspLI	GGNNCC	7 cut(s)	357, 441, 707, 1038, 1044, 1335, 1933
BspMAI	CTGCAG	1 cut(s)	405
BspPI	GGATC	7 cut(s)	311, 326, 326, 488, 700, 713, 1809
BsrBI	CCGCTC	1 cut(s)	933
BsrDI	GCAATG	2 cut(s)	954, 1098
BsrFI	RCCGGY	2 cut(s)	950, 1007
BsrGI	TGTACA	2 cut(s)	1542, 1612
BsrI	ACTGG	3 cut(s)	77, 1084, 1852
BssAI	RCCGGY	2 cut(s)	950, 1007
BssECI	CCNNGG	8 cut(s)	154, 338, 751, 752, 859, 871, 1040, 1988
BssMI	GATC	9 cut(s)	256, 303, 318, 331, 423, 493, 691, 705, 1801
BssNI	GRCGYC	2 cut(s)	183, 363
BssT1I	CCWWGG	1 cut(s)	1988
Bst2UI	CCWGG	2 cut(s)	753, 1489
Bst4CI	ACNGT	3 cut(s)	113, 783, 863
Bst6I	CTCTTC	4 cut(s)	39, 515, 606, 1232
BstACI	GRCGYC	2 cut(s)	183, 363
BstAPI	GCANNNNNTGC	1 cut(s)	1745
BstAUI	TGTACA	2 cut(s)	1542, 1612
BstC8I	GCNNGC	5 cut(s)	203, 717, 952, 1085, 1089
BstDSI	CCRYGG	2 cut(s)	859, 1988
BstF5I	GGATG	7 cut(s)	946, 1002, 1024, 1057, 1159, 1266, 1411
BstFNI	CGCG	1 cut(s)	516
BstH2I	RGCGCY	1 cut(s)	349
BstHHI	GCGC	2 cut(s)	312, 348
BstKTI	GATC	9 cut(s)	259, 306, 321, 334, 426, 496, 694, 708, 1804
BstMAI	GTCTC	2 cut(s)	413, 1776
BstMBI	GATC	9 cut(s)	256, 303, 318, 331, 423, 493, 691, 705, 1801
BstMCI	CGRYCG	3 cut(s)	417, 426, 453
BstNI	CCWGG	2 cut(s)	753, 1489
BstSCI	CCNGG	8 cut(s)	154, 338, 751, 881, 1039, 1275, 1487, 1635
BstSFI	CTRYAG	3 cut(s)	401, 775, 1677
BstSLI	GKGCMC	1 cut(s)	630
BstUI	CGCG	1 cut(s)	516
BstV1I	GCAGC	6 cut(s)	387, 471, 586, 889, 1111, 1288
BstV2I	GAAGAC	2 cut(s)	14, 1567
BstX2I	RGATCY	2 cut(s)	303, 705
BstYI	RGATCY	2 cut(s)	303, 705
Bsu36I	CCTNAGG	3 cut(s)	314, 1587, 1808
BtgI	CCRYGG	2 cut(s)	859, 1988
BtrI	CACGTC	1 cut(s)	796
BtsCI	GGATG	7 cut(s)	946, 1002, 1024, 1057, 1159, 1266, 1411
Cac8I	GCNNGC	5 cut(s)	203, 717, 952, 1085, 1089
CaiI	CAGNNNCTG	1 cut(s)	1901
CciI	TCATGA	2 cut(s)	811, 849
CfoI	GCGC	2 cut(s)	312, 348
Cfr10I	RCCGGY	2 cut(s)	950, 1007
CseI	GACGC	1 cut(s)	191
Csp6I	GTAC	3 cut(s)	1360, 1543, 1613
CspCI	CAANNNNNGTGG	2 cut(s)	1112, 1147
CviQI	GTAC	3 cut(s)	1360, 1543, 1613
DpnI	GATC	9 cut(s)	258, 305, 320, 333, 425, 495, 693, 707, 1803
DpnII	GATC	9 cut(s)	256, 303, 318, 331, 423, 493, 691, 705, 1801
DriI	GACNNNNNGTC	1 cut(s)	1839
Eam1104I	CTCTTC	4 cut(s)	39, 515, 606, 1232
Eam1105I	GACNNNNNGTC	1 cut(s)	1839
EarI	CTCTTC	4 cut(s)	39, 515, 606, 1232
EciI	GGCGGA	1 cut(s)	324
Ecl136II	GAGCTC	3 cut(s)	146, 205, 328
Eco130I	CCWWGG	1 cut(s)	1988
Eco24I	GRGCYC	4 cut(s)	142, 148, 207, 330
Eco32I	GATATC	1 cut(s)	1854
Eco47I	GGWCC	5 cut(s)	355, 1037, 1333, 1485, 1583
Eco47III	AGCGCT	1 cut(s)	347
Eco53kI	GAGCTC	3 cut(s)	146, 205, 328
Eco81I	CCTNAGG	3 cut(s)	314, 1587, 1808
Eco88I	CYCGRG	2 cut(s)	141, 870
EcoICRI	GAGCTC	3 cut(s)	146, 205, 328
EcoO109I	RGGNCCY	5 cut(s)	267, 355, 642, 749, 1485
EcoRI	GAATTC	1 cut(s)	547
EcoRII	CCWGG	2 cut(s)	751, 1487
EcoRV	GATATC	1 cut(s)	1854
EcoT14I	CCWWGG	1 cut(s)	1988
EcoT38I	GRGCYC	4 cut(s)	142, 148, 207, 330
ErhI	CCWWGG	1 cut(s)	1988
Esp3I	CGTCTC	1 cut(s)	413
FalI	AAGNNNNNCTT	2 cut(s)	715, 747
FaqI	GGGAC	4 cut(s)	206, 341, 889, 1050
FauI	CCCGC	3 cut(s)	926, 1096, 1278
FbaI	TGATCA	1 cut(s)	691
Fnu4HI	GCNGC	9 cut(s)	186, 401, 485, 488, 575, 903, 1097, 1100, 1302
FokI	GGATG	7 cut(s)	953, 1009, 1031, 1064, 1166, 1273, 1418
FriOI	GRGCYC	4 cut(s)	142, 148, 207, 330
Fsp4HI	GCNGC	9 cut(s)	186, 401, 485, 488, 575, 903, 1097, 1100, 1302
FspBI	CTAG	3 cut(s)	744, 1602, 1619
GlaI	GCGC	2 cut(s)	311, 347
GluI	GCNGC	9 cut(s)	186, 401, 485, 488, 575, 903, 1097, 1100, 1302
HaeII	RGCGCY	1 cut(s)	349
HapII	CCGG	9 cut(s)	156, 340, 374, 882, 951, 1008, 1040, 1277, 1636
HgaI	GACGC	1 cut(s)	191
HhaI	GCGC	2 cut(s)	312, 348
Hin1I	GRCGYC	2 cut(s)	183, 363
Hin6I	GCGC	2 cut(s)	310, 346
HinP1I	GCGC	2 cut(s)	310, 346
HindIII	AAGCTT	1 cut(s)	724
HinfI	GANTC	7 cut(s)	231, 764, 815, 841, 1169, 1355, 1834
HpaII	CCGG	9 cut(s)	156, 340, 374, 882, 951, 1008, 1040, 1277, 1636
HphI	GGTGA	8 cut(s)	52, 59, 199, 611, 1112, 1715, 1796, 1955
Hpy166II	GTNNAC	3 cut(s)	508, 700, 1333
Hpy188I	TCNGA	4 cut(s)	107, 196, 466, 769
Hpy188III	TCNNGA	7 cut(s)	316, 812, 850, 1549, 1586, 1734, 1895
Hpy8I	GTNNAC	3 cut(s)	508, 700, 1333
Hpy99I	CGWCG	9 cut(s)	200, 326, 365, 368, 431, 448, 451, 470, 973
HpyAV	CCTTC	6 cut(s)	50, 65, 583, 975, 1578, 1704
HpyCH4III	ACNGT	3 cut(s)	113, 783, 863
HpyCH4IV	ACGT	4 cut(s)	363, 510, 795, 989
HpyCH4V	TGCA	5 cut(s)	132, 403, 913, 1288, 1369
HpySE526I	ACGT	4 cut(s)	363, 510, 795, 989
Hsp92I	GRCGYC	2 cut(s)	183, 363
HspAI	GCGC	2 cut(s)	310, 346
KroI	GCCGGC	1 cut(s)	950
KroNI	GCCGGC	1 cut(s)	952
Ksp22I	TGATCA	1 cut(s)	691
Kzo9I	GATC	9 cut(s)	256, 303, 318, 331, 423, 493, 691, 705, 1801
LmnI	GCTCC	5 cut(s)	445, 930, 1159, 1766, 1931
Lsp1109I	GCAGC	6 cut(s)	387, 471, 586, 889, 1111, 1288
LweI	GCATC	6 cut(s)	931, 1009, 1251, 1275, 1396, 1597
MaeI	CTAG	3 cut(s)	744, 1602, 1619
MaeII	ACGT	4 cut(s)	363, 510, 795, 989
MaeIII	GTNAC	8 cut(s)	65, 113, 149, 1075, 1321, 1591, 1641, 1658
MalI	GATC	9 cut(s)	258, 305, 320, 333, 425, 495, 693, 707, 1803
MbiI	CCGCTC	1 cut(s)	933
MboI	GATC	9 cut(s)	256, 303, 318, 331, 423, 493, 691, 705, 1801
MboII	GAAGA	9 cut(s)	16, 19, 26, 254, 532, 598, 623, 1249, 1567
MflI	RGATCY	2 cut(s)	303, 705
MhlI	GDGCHC	6 cut(s)	142, 148, 207, 330, 630, 1227
MluCI	AATT	5 cut(s)	537, 547, 1135, 1516, 1925
MlyI	GAGTC	2 cut(s)	1349, 1828
MmeI	TCCRAC	2 cut(s)	219, 489
MroNI	GCCGGC	1 cut(s)	950
MroXI	GAANNNNTTC	1 cut(s)	347
MseI	TTAA	3 cut(s)	786, 1344, 1401
MspA1I	CMGCKG	3 cut(s)	577, 1102, 1793
MspI	CCGG	9 cut(s)	156, 340, 374, 882, 951, 1008, 1040, 1277, 1636
MspR9I	CCNGG	8 cut(s)	156, 340, 753, 883, 1041, 1277, 1489, 1637
Mva1269I	GAATGC	1 cut(s)	904
MvaI	CCWGG	2 cut(s)	753, 1489
MvnI	CGCG	1 cut(s)	516
NaeI	GCCGGC	1 cut(s)	952
NciI	CCSGG	6 cut(s)	156, 340, 883, 1041, 1277, 1637
NcoI	CCATGG	1 cut(s)	1988
NdeII	GATC	9 cut(s)	256, 303, 318, 331, 423, 493, 691, 705, 1801
NgoMIV	GCCGGC	1 cut(s)	950
NlaIV	GGNNCC	7 cut(s)	357, 441, 707, 1038, 1044, 1335, 1933
NmuCI	GTSAC	3 cut(s)	65, 1075, 1591
PagI	TCATGA	2 cut(s)	811, 849
PasI	CCCWGGG	1 cut(s)	752
PcsI	WCGNNNNNNNCGW	6 cut(s)	327, 357, 419, 443, 498, 507
PctI	GAATGC	1 cut(s)	904
PdiI	GCCGGC	1 cut(s)	952
PdmI	GAANNNNTTC	1 cut(s)	347
PfeI	GAWTC	5 cut(s)	231, 764, 815, 841, 1169
PkrI	GCNGC	9 cut(s)	187, 402, 486, 489, 576, 904, 1098, 1101, 1303
Ple19I	CGATCG	1 cut(s)	426
PleI	GAGTC	2 cut(s)	1349, 1828
PpsI	GAGTC	2 cut(s)	1349, 1828
PpuMI	RGGWCCY	2 cut(s)	355, 1485
Psp124BI	GAGCTC	3 cut(s)	148, 207, 330
Psp5II	RGGWCCY	2 cut(s)	355, 1485
Psp6I	CCWGG	2 cut(s)	751, 1487
PspGI	CCWGG	2 cut(s)	751, 1487
PspN4I	GGNNCC	7 cut(s)	357, 441, 707, 1038, 1044, 1335, 1933
PspPPI	RGGWCCY	2 cut(s)	355, 1485
PstI	CTGCAG	1 cut(s)	405
PstNI	CAGNNNCTG	1 cut(s)	1901
PsuI	RGATCY	2 cut(s)	303, 705
PvuI	CGATCG	1 cut(s)	426
PvuII	CAGCTG	3 cut(s)	577, 1102, 1793
RsaI	GTAC	3 cut(s)	1361, 1544, 1614
RsaNI	GTAC	3 cut(s)	1360, 1543, 1613
SacI	GAGCTC	3 cut(s)	148, 207, 330
SaqAI	TTAA	3 cut(s)	786, 1344, 1401
SatI	GCNGC	9 cut(s)	186, 401, 485, 488, 575, 903, 1097, 1100, 1302
Sau3AI	GATC	9 cut(s)	256, 303, 318, 331, 423, 493, 691, 705, 1801
SchI	GAGTC	2 cut(s)	1349, 1828
ScrFI	CCNGG	8 cut(s)	156, 340, 753, 883, 1041, 1277, 1489, 1637
SduI	GDGCHC	6 cut(s)	142, 148, 207, 330, 630, 1227
SfaNI	GCATC	6 cut(s)	931, 1009, 1251, 1275, 1396, 1597
SfcI	CTRYAG	3 cut(s)	401, 775, 1677
SinI	GGWCC	5 cut(s)	355, 1037, 1333, 1485, 1583
Sse9I	AATT	5 cut(s)	537, 547, 1135, 1516, 1925
SspMI	CTAG	3 cut(s)	744, 1602, 1619
SstI	GAGCTC	3 cut(s)	148, 207, 330
StyD4I	CCNGG	8 cut(s)	154, 338, 751, 881, 1039, 1275, 1487, 1635
StyI	CCWWGG	1 cut(s)	1988
TaaI	ACNGT	3 cut(s)	113, 783, 863
TaiI	ACGT	4 cut(s)	366, 513, 798, 992
TaqII	GACCGA	2 cut(s)	431, 439
TasI	AATT	5 cut(s)	537, 547, 1135, 1516, 1925
TatI	WGTACW	2 cut(s)	1542, 1612
TauI	GCSGC	3 cut(s)	188, 490, 1099
TfiI	GAWTC	5 cut(s)	231, 764, 815, 841, 1169
Tru1I	TTAA	3 cut(s)	786, 1344, 1401
Tru9I	TTAA	3 cut(s)	786, 1344, 1401
TseFI	GTSAC	3 cut(s)	65, 1075, 1591
TseI	GCWGC	6 cut(s)	400, 484, 574, 902, 1099, 1301
Tsp45I	GTSAC	3 cut(s)	65, 1075, 1591
TspDTI	ATGAA	5 cut(s)	17, 35, 800, 838, 1574
TspGWI	ACGGA	5 cut(s)	432, 486, 532, 647, 1185
VpaK11BI	GGWCC	5 cut(s)	355, 1037, 1333, 1485, 1583
XapI	RAATTY	1 cut(s)	547
XcmI	CCANNNNNNNNNTGG	1 cut(s)	84
XmnI	GAANNNNTTC	1 cut(s)	347
XspI	CTAG	3 cut(s)	744, 1602, 1619
ZraI	GACGTC	1 cut(s)	364

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.