Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0002632

Physical Location & Seq

Genomic Coordinates Reverse (-)

34980124 .. 34986338

Gene Structure 6215 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G023860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2769 bp

ATGAAGAAGACCCAGACGATGAACAACCTCACTCTTCTCCTTCTCCTCCTCCTCATCTATCACCTCTCCCCAGTTCTCTCATGGAAAAAAGACGAGTTCCGAAACTGTAACCAAACCCCTTTCTGCAAACGAGCGCGAGCTCGCAACCCCGGCTCGTCCTCCCTCACAGTCCAAGACGCCGCCATCTCCGACGGCGAGCTCACCGCCAAGCTCGTCCCTAAGAGAATCGAACAACAAGAAACAACCCCCGATCAAATCAAGCCCTTGCTTCTCACTCTCTCTGCTTACCAAGACGGGATCTTGCGCCTCAGGATCGACGAGCTCGATCCGCCCCGCATGCGCTTCGAGGTCCCCGACGTCGTTTTGCCGGAGTTCGAGAGCAAGAAGCTCTGGCTGCAGAGGCTTTCGACCGAGACGATCGACGGCGACGCGGCTCCGTCGTCAGTCGTGTTCTTATCCGACGGCTACGAGGCGGTGCTGCGGCACGATCCGTTCGAGGTTTACGTCCGCGAGAAGAGGGGTAATCGTGTAATTTCCGTGAATTCCAATGGCCTATTTGATTTTGAGCAGCTGAGAGAGAAGGGAGAGGGTGAGGATTGGGAAGAGAGGTTTAGAGGGCACACGGATAAGAGGCCCTACGGCCCTCAGTCCATTAGTTTTGATGTGTCCTTTTATGGGGCTGATCATGTTTATGGTATCCCTGAACGTGCTTCAAGCTTGGCTTTGAAGCCCACTAGAGGTCCCGGGGTTGAGGAATCTGAACCCTATAGGTTGTTTAACTTGGACGTGTTTGAGTATATTCATGATTCCCCATTCGGGCTTTACGGCTCGATTCCATTCATGATTTCCCACGGTAAGGCCCGAGGGACGTCCGGGTTCTTTTGGTTGAATGCTGCTGAAATGCAGATTGATGTTCTTGGAAGCGGGTGGGATGCCGAGGCAGGCATTGCTCTCCCTTCGTCGCAGAGTAGGATTGACACGTTTTGGATGAGTGAGGCCGGTATTGTCGATGCTTTTTTTTTCGTGGGACCGGGACCAAAGGATGTAGCACGTCAGTATGCGAGTGTGACTGGCACGCCCGCAATGCCGCAGCTTTTTGCGGTGGCGTATCACCAATGTAGGTGGAATTATAGAGACGAGGAGGATGTGGAGCAAGTTGATTCTAAGTTTGATGAGCATGATATTCCGTATGATGTTTTGTGGCTTGATATTGAGCACACAGACGGGAAGAGATATTTTACATGGGATAGGATGCTTTTCCCGCACCCGGAGGAGATGCAGAGGAAGTTGGCTGCTAAGGGTAGGCACATGGTTACTATTGTGGACCCTCATATTAAGCGAGATGACTCGTACTTTTTGCACAGGGAGGCCACAGAAAAAGGCTATTATGTTAAGGATGCCAATGGGAATGATTACGATGGGTGGTGCTGGTCTGGTTCGTCATCATATTTGGATATGTTGAGGCCAGAGGTTAGGTCCTGGTGGGCTACAAAGTTCTCTACTGAAAATTATGTTGGTTCAACTCCCTCCTTGTACATCTGGAATGACATGAATGAGCCTTCTGTCTTCAATGGTCCTGAGGTCACAATGCCTAGAGATGCTGTACATCTAGGAGATGTAGAACACCGGGAGTTACATAACGCCTATGGTTACTACTTTCACATGGCTACAGCAGATGGGCTTGTTCAGCGAGGTGATGGAAGGGATAGGCCATTTGTTTTATCAAGAGCAGTATTTGCTGGAAGTCAAAGGCATGGAGCAGTATGGACAGGAGACAATACAGCTGAATGGGATCACCTCAGGGTTTCAGTTCCGATGATTTTGACTCTTGGTCTTACTGGGATATCATTCTCTGGTGCTGATGTGGGTGGGTATTTTGGCAATCCGGAACCTGAGTTGTGGGTTCGTTGGTATCAATTAGGAGCCTACTATCCTTTCTTTAGAGGTCATGCTCACCATGACACCAAAAGACGAGAACCATGGTTATTTGGAGAAAAAAATACTGAACGTATAAGGGAGGCTATACACATCCGTTACATGTTGCTTCCATACTTTTACACATTATTCAGAGAGGCAAACACCAGTGGATTTCCAGTTGCTCGTCCATTGTGGATGGAGTTTCCTTCCGAGGAGGCTACTTTCACAAATGATGAGGCTCTCATGATTGGAAACAGTCTTTTGGTACAAGGGATCTATACAGAGCATGCAAAACATGCTTCTGTTTATTTGCCTGGGAAAGAATCATGGTATGATGTGAAAACTGGAGCAGCTTACAAGGGAGGTAAGACCCATAAGCTGGATGTTAATGAAGAGGGTGTTCCTGCTTTCCAGAGGGCTGGGACTATCATACCAAGGAAAGACCGCTTTCGTCGGAGTTCCACCCAGATGGTGAACGATCCATACACTCTGGTGATAGCACTGAATAGTTCACAAGCAGCTGAAGGTGAACTCTATGTGGATGATGGAAAAAGCTTCGAGTTTCAGCAAGGATCCTATATCCATCGTCGCTTTGTTTTCGCAGATGGGAAGCTTACATCTCTTAATCTGGCACCTGGACAAACCCAATTTTCTTCAGAATGTGTTATTGAGAGGATTATTCTGCAAGGTCTGTCTACGAGGCAAAAGAGTGCGCTAATTGAACCGGCAAATCAAAAGGCTGAGATTGAACGGGGCCCACTTCTCCTTTATTCAAGGCAGGGACCATCAGTGTTAACGGTTCGTAAGCCTAATGTTCGGATAGCAGATGATTGGGTCATAAAAATATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000902 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006491 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009250 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009987 GO:0010035 GO:0010038 GO:0012505 GO:0015926 GO:0016043 GO:0016049 GO:0016051 GO:0016787 GO:0016798 GO:0019538 GO:0030243 GO:0030244 GO:0030587 GO:0031288 GO:0032502 GO:0032989 GO:0033692 GO:0033919 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043687 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0051274 GO:0051703 GO:0051704 GO:0051707 GO:0060560 GO:0071704 GO:0071840 GO:0090599 GO:0090600 GO:0090702 GO:0098542 GO:0099120 GO:1901135 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

922

Amino Acids

105.24

Weight (kDa)

5.72

Isoelectric Point (pI)

42.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	91 - 304	9.5e-41	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	360 - 688	9.3e-120	Glycosyl hydrolases family 31 TIM-barrel domain
Glyco_hydro_31_3rd	PF21365	696 - 783	3.6e-23	Glycosyl hydrolase family 31 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63840 AT5G63840
fragaria_vesca	FvH4_1g25070
malus_domestica	MD01G1007800.v1.1 MD01G1007900.v1.1 MD15G1135800.v1.1
prunus_persica	Prupe.4G277800_v2.0.a1 Prupe.4G277800_v2.0.a1
pyrus_communis	pycom15g12240 pycom520g00330
rosa_chinensis	RchiOBHm_Chr2g0122711 RchiOBHm_Chr2g0122721
rosa_laevigata	RLG00000018707 RLG00000018709
rosa_multiflora	Rmu_sc0005370.1_g000002 Rmu_sc0008259.1_g000009 Rmu_sc0008259.1_g000014 Rmu_sc0017734.1_g000006 Rmu_sc0026486.1_g000001
rosa_roxburghii	Rroxscaffold_1G00031190 Rroxscaffold_2G00121270 Rroxscaffold_2G00121280 Rroxscaffold_4G00297410 Rroxscaffold_4G00317480 Rroxscaffold_4G00325980 Rroxscaffold_7G00197700 Rroxscaffold_7G00199130
rosa_rugosa	Rorug02G0241800 Rorug02G0241800 Rorug02G0241900 Rorug02G0242000 Rorug02G0242100
rosa_samantha	Rh2AG300300 Rh2BG308200 Rh2CG286400 Rh2CG286900 Rh2DG324500
rosa_wichuraiana	Rw2G023850 Rw2G023860 Rw2G024040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	360, 872
AccB1I	GGYRCC	1 cut(s)	2548
AccB7I	CCANNNNNTGG	1 cut(s)	2296
AccI	GTMKAC	1 cut(s)	2612
AccII	CGCG	3 cut(s)	136, 431, 510
AccIII	TCCGGA	1 cut(s)	1885
AclWI	GGATC	9 cut(s)	305, 320, 320, 482, 1800, 2198, 2390, 2484, 2497
AcsI	RAATTY	1 cut(s)	541
AcuI	CTGAAG	2 cut(s)	2460, 2556
AcyI	GRCGYC	3 cut(s)	177, 357, 869
AfaI	GTAC	4 cut(s)	1352, 1535, 1605, 2184
AfiI	CCNNNNNNNGG	6 cut(s)	675, 737, 816, 856, 1267, 2296
AflIII	ACRYGT	3 cut(s)	786, 978, 2037
AgsI	TTSAA	8 cut(s)	714, 727, 889, 1521, 1570, 2639, 2666, 2691
AhdI	GACNNNNNGTC	1 cut(s)	1830
AjiI	CACGTC	2 cut(s)	787, 1052
AjnI	CCWGG	3 cut(s)	1478, 2230, 2551
AjuI	GAANNNNNNNTTGG	2 cut(s)	1497, 1529
AleI	CACNNNNGTG	1 cut(s)	2408
Alw21I	GWGCWC	4 cut(s)	142, 201, 324, 1218
Alw26I	GTCTC	3 cut(s)	407, 1128, 1767
AlwI	GGATC	9 cut(s)	305, 320, 320, 482, 1800, 2198, 2390, 2484, 2497
Ama87I	CYCGRG	2 cut(s)	743, 861
Aor13HI	TCCGGA	1 cut(s)	1885
AoxI	GGCC	9 cut(s)	550, 632, 640, 858, 996, 1368, 1463, 1709, 2671
ApaI	GGGCCC	1 cut(s)	2675
ApeKI	GCWGC	8 cut(s)	394, 478, 568, 893, 1090, 1292, 2267, 2435
ApoI	RAATTY	1 cut(s)	541
AspLEI	GCGC	4 cut(s)	136, 306, 342, 2632
AsuC2I	CCSGG	7 cut(s)	150, 744, 745, 874, 1032, 1268, 1628
AvaI	CYCGRG	2 cut(s)	743, 861
AvaII	GGWCC	8 cut(s)	349, 740, 1028, 1034, 1324, 1476, 1574, 2699
AxyI	CCTNAGG	3 cut(s)	308, 1578, 1799
BaeGI	GKGCMC	2 cut(s)	621, 2675
BamHI	GGATCC	1 cut(s)	2489
BanI	GGYRCC	1 cut(s)	2548
BanII	GRGCYC	4 cut(s)	142, 201, 324, 2675
BbsI	GAAGAC	2 cut(s)	14, 1558
Bbv12I	GWGCWC	4 cut(s)	142, 201, 324, 1218
BbvI	GCAGC	8 cut(s)	381, 465, 580, 880, 1102, 1279, 2279, 2447
BceAI	ACGGC	5 cut(s)	208, 439, 478, 655, 841
BcgI	CGANNNNNNTGC	2 cut(s)	325, 359
BciT130I	CCWGG	3 cut(s)	1480, 2232, 2553
BciVI	GTATCC	1 cut(s)	707
BclI	TGATCA	1 cut(s)	682
BcnI	CCSGG	7 cut(s)	150, 744, 745, 874, 1032, 1268, 1628
BcoDI	GTCTC	3 cut(s)	407, 1128, 1767
BfaI	CTAG	3 cut(s)	735, 1593, 1610
BfmI	CTRYAG	3 cut(s)	395, 766, 1668
BfuI	GTATCC	1 cut(s)	707
Bme18I	GGWCC	8 cut(s)	349, 740, 1028, 1034, 1324, 1476, 1574, 2699
BmeRI	GACNNNNNGTC	1 cut(s)	1830
BmeT110I	CYCGRG	2 cut(s)	743, 861
BmgBI	CACGTC	2 cut(s)	787, 1052
BmrI	ACTGGG	2 cut(s)	65, 1848
BmsI	GCATC	6 cut(s)	922, 1000, 1242, 1266, 1387, 1588
BmuI	ACTGGG	2 cut(s)	65, 1848
BpiI	GAAGAC	2 cut(s)	14, 1558
BpmI	CTGGAG	1 cut(s)	2283
Bpu10I	CCTNAGC	1 cut(s)	1296
BpuMI	CCSGG	7 cut(s)	150, 744, 745, 874, 1032, 1268, 1628
BsaHI	GRCGYC	3 cut(s)	177, 357, 869
BsaWI	WCCGGW	1 cut(s)	1885
Bsc4I	CCNNNNNNNGG	6 cut(s)	675, 737, 816, 856, 1267, 2296
Bse118I	RCCGGY	2 cut(s)	998, 2641
Bse1I	ACTGG	6 cut(s)	71, 1075, 1843, 2082, 2093, 2266
Bse21I	CCTNAGG	3 cut(s)	308, 1578, 1799
Bse3DI	GCAATG	2 cut(s)	945, 1089
BseAI	TCCGGA	1 cut(s)	1885
BseBI	CCWGG	3 cut(s)	1480, 2232, 2553
BseLI	CCNNNNNNNGG	6 cut(s)	675, 737, 816, 856, 1267, 2296
BseMI	GCAATG	2 cut(s)	945, 1089
BseMII	CTCAG	7 cut(s)	322, 563, 659, 1569, 1813, 1884, 2649
BseNI	ACTGG	6 cut(s)	71, 1075, 1843, 2082, 2093, 2266
BseRI	GAGGAG	6 cut(s)	35, 38, 41, 1154, 1286, 2144
BseSI	GKGCMC	2 cut(s)	621, 2675
BseXI	GCAGC	8 cut(s)	381, 465, 580, 880, 1102, 1279, 2279, 2447
BseYI	CCCAGC	1 cut(s)	2336
Bsh1236I	CGCG	3 cut(s)	136, 431, 510
Bsh1285I	CGRYCG	2 cut(s)	411, 420
BshFI	GGCC	9 cut(s)	552, 634, 642, 860, 998, 1370, 1465, 1711, 2673
BshNI	GGYRCC	1 cut(s)	2548
BsiEI	CGRYCG	2 cut(s)	411, 420
BsiHKAI	GWGCWC	4 cut(s)	142, 201, 324, 1218
BsiHKCI	CYCGRG	2 cut(s)	743, 861
BslFI	GGGAC	8 cut(s)	200, 335, 726, 880, 1041, 1047, 2353, 2712
BslI	CCNNNNNNNGG	6 cut(s)	675, 737, 816, 856, 1267, 2296
BsmAI	GTCTC	3 cut(s)	407, 1128, 1767
BsmBI	CGTCTC	2 cut(s)	407, 1128
BsmFI	GGGAC	8 cut(s)	200, 335, 726, 880, 1041, 1047, 2353, 2712
BsmI	GAATGC	1 cut(s)	895
BsnI	GGCC	9 cut(s)	552, 634, 642, 860, 998, 1370, 1465, 1711, 2673
BsoBI	CYCGRG	2 cut(s)	743, 861
Bsp120I	GGGCCC	1 cut(s)	2671
Bsp1286I	GDGCHC	6 cut(s)	142, 201, 324, 621, 1218, 2675
Bsp13I	TCCGGA	1 cut(s)	1885
Bsp1407I	TGTACA	2 cut(s)	1533, 1603
Bsp19I	CCATGG	1 cut(s)	1979
BspANI	GGCC	9 cut(s)	552, 634, 642, 860, 998, 1370, 1465, 1711, 2673
BspCNI	CTCAG	7 cut(s)	321, 564, 658, 1570, 1812, 1885, 2650
BspEI	TCCGGA	1 cut(s)	1885
BspFNI	CGCG	3 cut(s)	136, 431, 510
BspHI	TCATGA	3 cut(s)	802, 840, 2160
BspMAI	CTGCAG	1 cut(s)	399
BspPI	GGATC	9 cut(s)	305, 320, 320, 482, 1800, 2198, 2390, 2484, 2497
BspT107I	GGYRCC	1 cut(s)	2548
BsrDI	GCAATG	2 cut(s)	945, 1089
BsrFI	RCCGGY	2 cut(s)	998, 2641
BsrGI	TGTACA	2 cut(s)	1533, 1603
BsrI	ACTGG	6 cut(s)	71, 1075, 1843, 2082, 2093, 2266
BssAI	RCCGGY	2 cut(s)	998, 2641
BssNI	GRCGYC	3 cut(s)	177, 357, 869
BssT1I	CCWWGG	2 cut(s)	1979, 2351
Bst2UI	CCWGG	3 cut(s)	1480, 2232, 2553
Bst4CI	ACNGT	5 cut(s)	107, 169, 854, 2174, 2716
Bst6I	CTCTTC	5 cut(s)	39, 509, 597, 1223, 2304
BstACI	GRCGYC	3 cut(s)	177, 357, 869
BstAPI	GCANNNNNTGC	3 cut(s)	947, 1736, 2213
BstAUI	TGTACA	2 cut(s)	1533, 1603
BstC8I	GCNNGC	8 cut(s)	138, 142, 197, 338, 943, 1076, 1080, 2205
BstDSI	CCRYGG	2 cut(s)	850, 1979
BstFNI	CGCG	3 cut(s)	136, 431, 510
BstHHI	GCGC	4 cut(s)	136, 306, 342, 2632
BstMAI	GTCTC	3 cut(s)	407, 1128, 1767
BstMCI	CGRYCG	2 cut(s)	411, 420
BstNI	CCWGG	3 cut(s)	1480, 2232, 2553
BstNSI	RCATGY	4 cut(s)	340, 2041, 2207, 2216
BstSFI	CTRYAG	3 cut(s)	395, 766, 1668
BstSLI	GKGCMC	2 cut(s)	621, 2675
BstUI	CGCG	3 cut(s)	136, 431, 510
BstV1I	GCAGC	8 cut(s)	381, 465, 580, 880, 1102, 1279, 2279, 2447
BstV2I	GAAGAC	2 cut(s)	14, 1558
BstX2I	RGATCY	3 cut(s)	297, 2190, 2489
BstXI	CCANNNNNNTGG	2 cut(s)	2336, 2386
BstYI	RGATCY	3 cut(s)	297, 2190, 2489
Bsu36I	CCTNAGG	3 cut(s)	308, 1578, 1799
BsuI	GTATCC	1 cut(s)	707
BsuRI	GGCC	9 cut(s)	552, 634, 642, 860, 998, 1370, 1465, 1711, 2673
BtgI	CCRYGG	2 cut(s)	850, 1979
BtrI	CACGTC	2 cut(s)	787, 1052
BtsIMutI	CAGTG	3 cut(s)	2089, 2417, 2712
Cac8I	GCNNGC	8 cut(s)	138, 142, 197, 338, 943, 1076, 1080, 2205
CciI	TCATGA	3 cut(s)	802, 840, 2160
CfoI	GCGC	4 cut(s)	136, 306, 342, 2632
Cfr10I	RCCGGY	2 cut(s)	998, 2641
Cfr9I	CCCGGG	1 cut(s)	743
CseI	GACGC	2 cut(s)	185, 437
Csp6I	GTAC	4 cut(s)	1351, 1534, 1604, 2183
CspCI	CAANNNNNGTGG	2 cut(s)	1103, 1138
CviQI	GTAC	4 cut(s)	1351, 1534, 1604, 2183
DriI	GACNNNNNGTC	1 cut(s)	1830
Eam1104I	CTCTTC	5 cut(s)	39, 509, 597, 1223, 2304
Eam1105I	GACNNNNNGTC	1 cut(s)	1830
EarI	CTCTTC	5 cut(s)	39, 509, 597, 1223, 2304
EciI	GGCGGA	1 cut(s)	318
Ecl136II	GAGCTC	3 cut(s)	140, 199, 322
Eco130I	CCWWGG	2 cut(s)	1979, 2351
Eco24I	GRGCYC	4 cut(s)	142, 201, 324, 2675
Eco32I	GATATC	1 cut(s)	1845
Eco47I	GGWCC	8 cut(s)	349, 740, 1028, 1034, 1324, 1476, 1574, 2699
Eco53kI	GAGCTC	3 cut(s)	140, 199, 322
Eco57I	CTGAAG	2 cut(s)	2460, 2556
Eco81I	CCTNAGG	3 cut(s)	308, 1578, 1799
Eco88I	CYCGRG	2 cut(s)	743, 861
EcoICRI	GAGCTC	3 cut(s)	140, 199, 322
EcoO109I	RGGNCCY	5 cut(s)	349, 633, 740, 1476, 2671
EcoRI	GAATTC	1 cut(s)	541
EcoRII	CCWGG	3 cut(s)	1478, 2230, 2551
EcoRV	GATATC	1 cut(s)	1845
EcoT14I	CCWWGG	2 cut(s)	1979, 2351
EcoT38I	GRGCYC	4 cut(s)	142, 201, 324, 2675
ErhI	CCWWGG	2 cut(s)	1979, 2351
Esp3I	CGTCTC	2 cut(s)	407, 1128
FalI	AAGNNNNNCTT	2 cut(s)	706, 738
FaqI	GGGAC	8 cut(s)	200, 335, 726, 880, 1041, 1047, 2353, 2712
FauI	CCCGC	4 cut(s)	341, 917, 1087, 1269
FbaI	TGATCA	1 cut(s)	682
FblI	GTMKAC	1 cut(s)	2612
FriOI	GRGCYC	4 cut(s)	142, 201, 324, 2675
FspBI	CTAG	3 cut(s)	735, 1593, 1610
GlaI	GCGC	4 cut(s)	135, 305, 341, 2631
GsaI	CCCAGC	1 cut(s)	2340
GsuI	CTGGAG	1 cut(s)	2283
HaeIII	GGCC	9 cut(s)	552, 634, 642, 860, 998, 1370, 1465, 1711, 2673
HgaI	GACGC	2 cut(s)	185, 437
HhaI	GCGC	4 cut(s)	136, 306, 342, 2632
Hin1I	GRCGYC	3 cut(s)	177, 357, 869
Hin6I	GCGC	4 cut(s)	134, 304, 340, 2630
HinP1I	GCGC	4 cut(s)	134, 304, 340, 2630
HincII	GTYRAC	1 cut(s)	2712
HindII	GTYRAC	1 cut(s)	2712
HindIII	AAGCTT	3 cut(s)	715, 2470, 2528
HinfI	GANTC	8 cut(s)	225, 755, 806, 832, 1160, 1346, 1825, 2240
HpaI	GTTAAC	1 cut(s)	2712
Hpy166II	GTNNAC	7 cut(s)	502, 1324, 2392, 2429, 2447, 2613, 2712
Hpy8I	GTNNAC	7 cut(s)	502, 1324, 2392, 2429, 2447, 2613, 2712
HpyAV	CCTTC	7 cut(s)	50, 574, 966, 1569, 1695, 2133, 2435
HpyCH4III	ACNGT	5 cut(s)	107, 169, 854, 2174, 2716
HpyCH4IV	ACGT	8 cut(s)	357, 504, 706, 786, 869, 980, 1051, 2008
HpyCH4V	TGCA	7 cut(s)	126, 397, 904, 1279, 1360, 2207, 2602
HpySE526I	ACGT	8 cut(s)	357, 504, 706, 786, 869, 980, 1051, 2008
Hsp92I	GRCGYC	3 cut(s)	177, 357, 869
HspAI	GCGC	4 cut(s)	134, 304, 340, 2630
Kpn2I	TCCGGA	1 cut(s)	1885
Ksp22I	TGATCA	1 cut(s)	682
KspAI	GTTAAC	1 cut(s)	2712
LmnI	GCTCC	5 cut(s)	439, 1150, 1757, 1922, 2264
Lsp1109I	GCAGC	8 cut(s)	381, 465, 580, 880, 1102, 1279, 2279, 2447
LweI	GCATC	6 cut(s)	922, 1000, 1242, 1266, 1387, 1588
MaeI	CTAG	3 cut(s)	735, 1593, 1610
MaeII	ACGT	8 cut(s)	357, 504, 706, 786, 869, 980, 1051, 2008
MaeIII	GTNAC	7 cut(s)	107, 1066, 1312, 1582, 1632, 1649, 2033
MboII	GAAGA	9 cut(s)	16, 19, 26, 526, 614, 1240, 1558, 2321, 2562
MflI	RGATCY	3 cut(s)	297, 2190, 2489
MhlI	GDGCHC	6 cut(s)	142, 201, 324, 621, 1218, 2675
MluCI	AATT	7 cut(s)	531, 541, 1126, 1507, 1916, 2564, 2634
MlyI	GAGTC	2 cut(s)	1340, 1819
MmeI	TCCRAC	3 cut(s)	213, 483, 2351
MroI	TCCGGA	1 cut(s)	1885
MseI	TTAA	6 cut(s)	777, 1335, 1392, 2304, 2541, 2711
MslI	CAYNNNNRTG	3 cut(s)	690, 2384, 2408
MspA1I	CMGCKG	3 cut(s)	571, 1784, 2438
Mva1269I	GAATGC	1 cut(s)	895
MvaI	CCWGG	3 cut(s)	1480, 2232, 2553
MvnI	CGCG	3 cut(s)	136, 431, 510
NciI	CCSGG	7 cut(s)	150, 744, 745, 874, 1032, 1268, 1628
NcoI	CCATGG	1 cut(s)	1979
NmeAIII	GCCGAG	1 cut(s)	961
NmuCI	GTSAC	2 cut(s)	1066, 1582
NspI	RCATGY	4 cut(s)	340, 2041, 2207, 2216
OliI	CACNNNNGTG	1 cut(s)	2408
PaeI	GCATGC	2 cut(s)	340, 2207
PagI	TCATGA	3 cut(s)	802, 840, 2160
PciI	ACATGT	1 cut(s)	2037
PcsI	WCGNNNNNNNCGW	5 cut(s)	321, 351, 413, 492, 501
PctI	GAATGC	1 cut(s)	895
PfeI	GAWTC	6 cut(s)	225, 755, 806, 832, 1160, 2240
PflMI	CCANNNNNTGG	1 cut(s)	2296
Ple19I	CGATCG	1 cut(s)	420
PleI	GAGTC	2 cut(s)	1340, 1819
PpsI	GAGTC	2 cut(s)	1340, 1819
PpuMI	RGGWCCY	3 cut(s)	349, 740, 1476
PscI	ACATGT	1 cut(s)	2037
Psp124BI	GAGCTC	3 cut(s)	142, 201, 324
Psp5II	RGGWCCY	3 cut(s)	349, 740, 1476
Psp6I	CCWGG	3 cut(s)	1478, 2230, 2551
PspFI	CCCAGC	1 cut(s)	2336
PspGI	CCWGG	3 cut(s)	1478, 2230, 2551
PspOMI	GGGCCC	1 cut(s)	2671
PspPPI	RGGWCCY	3 cut(s)	349, 740, 1476
PsrI	GAACNNNNNNTAC	2 cut(s)	2384, 2416
PstI	CTGCAG	1 cut(s)	399
PsuI	RGATCY	3 cut(s)	297, 2190, 2489
PvuI	CGATCG	1 cut(s)	420
PvuII	CAGCTG	3 cut(s)	571, 1784, 2438
RsaI	GTAC	4 cut(s)	1352, 1535, 1605, 2184
RsaNI	GTAC	4 cut(s)	1351, 1534, 1604, 2183
RseI	CAYNNNNRTG	3 cut(s)	690, 2384, 2408
SacI	GAGCTC	3 cut(s)	142, 201, 324
SaqAI	TTAA	6 cut(s)	777, 1335, 1392, 2304, 2541, 2711
SchI	GAGTC	2 cut(s)	1340, 1819
SduI	GDGCHC	6 cut(s)	142, 201, 324, 621, 1218, 2675
SfaNI	GCATC	6 cut(s)	922, 1000, 1242, 1266, 1387, 1588
SfcI	CTRYAG	3 cut(s)	395, 766, 1668
SinI	GGWCC	8 cut(s)	349, 740, 1028, 1034, 1324, 1476, 1574, 2699
SmaI	CCCGGG	1 cut(s)	745
SmiMI	CAYNNNNRTG	3 cut(s)	690, 2384, 2408
SphI	GCATGC	2 cut(s)	340, 2207
Sse9I	AATT	7 cut(s)	531, 541, 1126, 1507, 1916, 2564, 2634
SspI	AATATT	1 cut(s)	2763
SspMI	CTAG	3 cut(s)	735, 1593, 1610
SstI	GAGCTC	3 cut(s)	142, 201, 324
StyI	CCWWGG	2 cut(s)	1979, 2351
TaaI	ACNGT	5 cut(s)	107, 169, 854, 2174, 2716
TaiI	ACGT	8 cut(s)	360, 507, 709, 789, 872, 983, 1054, 2011
TaqII	GACCGA	1 cut(s)	425
TasI	AATT	7 cut(s)	531, 541, 1126, 1507, 1916, 2564, 2634
TatI	WGTACW	2 cut(s)	1533, 1603
TauI	GCSGC	4 cut(s)	182, 434, 484, 1090
TfiI	GAWTC	6 cut(s)	225, 755, 806, 832, 1160, 2240
Tru1I	TTAA	6 cut(s)	777, 1335, 1392, 2304, 2541, 2711
Tru9I	TTAA	6 cut(s)	777, 1335, 1392, 2304, 2541, 2711
TscAI	CASTG	3 cut(s)	2089, 2424, 2712
TseFI	GTSAC	2 cut(s)	1066, 1582
TseI	GCWGC	8 cut(s)	394, 478, 568, 893, 1090, 1292, 2267, 2435
Tsp45I	GTSAC	2 cut(s)	1066, 1582
TspDTI	ATGAA	6 cut(s)	17, 35, 791, 829, 1565, 2322
TspGWI	ACGGA	6 cut(s)	426, 480, 526, 638, 1176, 2021
TspMI	CCCGGG	1 cut(s)	743
TspRI	CASTG	3 cut(s)	2089, 2424, 2712
Van91I	CCANNNNNTGG	1 cut(s)	2296
VpaK11BI	GGWCC	8 cut(s)	349, 740, 1028, 1034, 1324, 1476, 1574, 2699
XapI	RAATTY	1 cut(s)	541
XceI	RCATGY	4 cut(s)	340, 2041, 2207, 2216
XcmI	CCANNNNNNNNNTGG	1 cut(s)	78
XmaI	CCCGGG	1 cut(s)	743
XmiI	GTMKAC	1 cut(s)	2612
XspI	CTAG	3 cut(s)	735, 1593, 1610
ZraI	GACGTC	2 cut(s)	358, 870

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.