Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0002632

Physical Location & Seq

Genomic Coordinates Forward (+)

4094832 .. 4097085

Gene Structure 2254 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1007800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1377 bp

ATGAAGATGGAGAGGCAGGTCAGAATTACCAAGATGGGATCTTGCGGCTCAAGATCGACGAGGACCAAACTCGATCCACCCAAGAAGCGTTCCGAGGTTCCCGATGTGCTTGTGCCGGAGTTTTTTAGCAAGAAGTTGTGGTTGCAGAGGCTTTCAACAGAGACCATCGGCGGCAATGTAGGTCCGTCGTCGATCGTCTACTTGTTGGACGGCTACGAGGCGGTGCTCCGGCACGATCCCTTTGAGGTTTACGTGCGCAAAAAGGGAGGTAATCGCATAGTTTCTATGAATTCTCATGGTTTATTTGATTTTGAGCAATTGAGGGAGAAGAATGGTGAGGATTGGCAGGAGAGGTTCAAGGGGCATACAGATACGAGGCCCTTCGGCCCGCAATCGATTAGCTTCGACGTGTCCTTTTATGATGCTGATCATGTGTATGGGATTCCTGAGCGTGCTACAAGCTTCGCTCTGAAGCCCACTAGAGGTCCTGGGGTTGAGGAATCTGAACCCTATAGATTGTTTAACTTAGATGTGTTTGAGTACATTCATGATTCCCCATTCGGACTTTATGGTTCTGTACCCTTAATGATTTCCCACGGCAAGGCGAGCGGGACTTCCGGTTTCTTTTGGTTGAATGCTGCTGAGATGCAGATTGATGTTCTTGGAACCGGCTGGGATGCAGAGTCTGGCATTTCTCCCCCTACGTCACAGAATAGGATCGATACACATTGGATGAGTGAGGCAGGTATTGTCGATGCCTTCTTTTTCGTAGGACCTGGACCTAAGGATGTAATCGGCCAGTACACGAGTGTAACTGGAACACCTGCAATGCCACAGTTGTTTGCAGTGGCCTATCACCAATGTAGGTGGAATTACCGGGATGAGGAGGATGTTGAGCAAGTTGATGCTATGTTTGACGAGCACGATATCCCATATGATGTTCTGTGGCTTGATATTGAGCACACAGATGGGAAGAGATACTTTACATGGGATAGAACGCTTTTTCCACACCCAGAGGAGATGCAGAGGAAGTTGGCTGGTAAGGGTAGGCACATGGTTACTATTGTGGATCCTCATATTAAGCGTGTTGATTCCTATTTTTTGCACAAACAGGCCACAGAAAAGCGGTATTATGTTAGGGATAACACTGGAAAAGATTATGATGGGTGGTGCTGGCCTGGTTCATCATCTTATTTGGATATGTTGAGGCCAGAGGTTAGGTCCTGGTGGGCTGAAAAGTTCTCTATTGAGAATTATGTTGGTTCAACTCCTTCACTGTACATCTGGAATGACATGAATGAGCCTTCTGTCTTCAATGGTCCCGAGGCATCAAATGCTATATTTGTGGTTTTCAATCGAACCCCTGTGCTAACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000902 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005975 GO:0005976 GO:0006073 GO:0006464 GO:0006491 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009100 GO:0009250 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009653 GO:0009826 GO:0009987 GO:0010035 GO:0010038 GO:0012505 GO:0015926 GO:0016043 GO:0016049 GO:0016051 GO:0016787 GO:0016798 GO:0019538 GO:0030243 GO:0030244 GO:0030587 GO:0031288 GO:0032502 GO:0032989 GO:0033692 GO:0033919 GO:0034637 GO:0034645 GO:0036211 GO:0040007 GO:0042221 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043687 GO:0044042 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0048589 GO:0048856 GO:0048869 GO:0050896 GO:0051273 GO:0051274 GO:0051703 GO:0051704 GO:0051707 GO:0060560 GO:0071704 GO:0071840 GO:0090599 GO:0090600 GO:0090702 GO:0098542 GO:0099120 GO:1901135 GO:1901564 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00510 ko01100 ko04141 map00510 map01100 map04141

Pfam Domains

Protein Families

DUF5110 Gal_mutarotas_2 Glyco_hydro_31

Protein Analysis

459

Amino Acids

52.56

Weight (kDa)

5.51

Isoelectric Point (pI)

44.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gal_mutarotas_2	PF13802	66 - 219	8.1e-37	Glycosyl hydrolase 31 N-terminal galactose mutarotase-like domain
Glyco_hydro_31_2nd	PF01055	275 - 442	1.5e-50	Glycosyl hydrolases family 31 TIM-barrel domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002632)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G63840 AT5G63840
fragaria_vesca	FvH4_1g25070
malus_domestica	MD01G1007800.v1.1 MD01G1007900.v1.1 MD15G1135800.v1.1
prunus_persica	Prupe.4G277800_v2.0.a1 Prupe.4G277800_v2.0.a1
pyrus_communis	pycom15g12240 pycom520g00330
rosa_chinensis	RchiOBHm_Chr2g0122711 RchiOBHm_Chr2g0122721
rosa_laevigata	RLG00000018707 RLG00000018709
rosa_multiflora	Rmu_sc0005370.1_g000002 Rmu_sc0008259.1_g000009 Rmu_sc0008259.1_g000014 Rmu_sc0017734.1_g000006 Rmu_sc0026486.1_g000001
rosa_roxburghii	Rroxscaffold_1G00031190 Rroxscaffold_2G00121270 Rroxscaffold_2G00121280 Rroxscaffold_4G00297410 Rroxscaffold_4G00317480 Rroxscaffold_4G00325980 Rroxscaffold_7G00197700 Rroxscaffold_7G00199130
rosa_rugosa	Rorug02G0241800 Rorug02G0241800 Rorug02G0241900 Rorug02G0242000 Rorug02G0242100
rosa_samantha	Rh2AG300300 Rh2BG308200 Rh2CG286400 Rh2CG286900 Rh2DG324500
rosa_wichuraiana	Rw2G023850 Rw2G023860 Rw2G024040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	832
Acc16I	TGCGCA	1 cut(s)	257
Acc36I	ACCTGC	3 cut(s)	7, 734, 832
AccBSI	CCGCTC	1 cut(s)	609
AccI	GTMKAC	1 cut(s)	198
AciI	CCGC	6 cut(s)	45, 171, 221, 389, 609, 1126
AclWI	GGATC	6 cut(s)	46, 68, 230, 725, 1064, 1077
AcoI	YGGCCR	1 cut(s)	796
AcsI	RAATTY	1 cut(s)	289
AcuI	CTGAAG	1 cut(s)	491
AfaI	GTAC	4 cut(s)	542, 579, 803, 1280
AfiI	CCNNNNNNNGG	3 cut(s)	482, 601, 883
AflIII	ACRYGT	1 cut(s)	408
AgsI	TTSAA	6 cut(s)	156, 358, 634, 1266, 1315, 1354
AjiI	CACGTC	1 cut(s)	409
AjnI	CCWGG	4 cut(s)	487, 775, 1177, 1223
AloI	GAACNNNNNNTCC	2 cut(s)	988, 1020
AluBI	AGCT	2 cut(s)	402, 462
AluI	AGCT	2 cut(s)	402, 462
Alw21I	GWGCWC	3 cut(s)	228, 924, 963
Alw26I	GTCTC	1 cut(s)	155
AlwI	GGATC	6 cut(s)	46, 68, 230, 725, 1064, 1077
AlwNI	CAGNNNCTG	1 cut(s)	686
Ama87I	CYCGRG	1 cut(s)	1322
AoxI	GGCC	7 cut(s)	377, 385, 796, 849, 1113, 1175, 1208
ApeKI	GCWGC	1 cut(s)	638
ApoI	RAATTY	1 cut(s)	289
AspLEI	GCGC	1 cut(s)	258
AspS9I	GGNCC	9 cut(s)	63, 182, 378, 386, 485, 773, 779, 1221, 1319
AsuC2I	CCSGG	1 cut(s)	878
AsuHPI	GGTGA	2 cut(s)	347, 848
AvaI	CYCGRG	1 cut(s)	1322
AvaII	GGWCC	7 cut(s)	63, 182, 485, 773, 779, 1221, 1319
AxyI	CCTNAGG	1 cut(s)	783
BamHI	GGATCC	1 cut(s)	1069
BauI	CACGAG	1 cut(s)	805
BbsI	GAAGAC	1 cut(s)	1303
Bbv12I	GWGCWC	3 cut(s)	228, 924, 963
BbvI	GCAGC	1 cut(s)	625
BccI	CCATC	4 cut(s)	28, 173, 962, 1157
BceAI	ACGGC	2 cut(s)	226, 613
BciT130I	CCWGG	4 cut(s)	489, 777, 1179, 1225
BclI	TGATCA	1 cut(s)	427
BcnI	CCSGG	1 cut(s)	878
BcoDI	GTCTC	1 cut(s)	155
BfaI	CTAG	1 cut(s)	480
BfmI	CTRYAG	1 cut(s)	511
BfuAI	ACCTGC	3 cut(s)	7, 734, 832
BisI	GCNGC	3 cut(s)	46, 172, 639
BlsI	GCNGC	3 cut(s)	47, 173, 640
Bme1390I	CCNGG	5 cut(s)	489, 777, 878, 1179, 1225
Bme18I	GGWCC	7 cut(s)	63, 182, 485, 773, 779, 1221, 1319
BmeT110I	CYCGRG	1 cut(s)	1322
BmgBI	CACGTC	1 cut(s)	409
BmgT120I	GGNCC	9 cut(s)	63, 182, 378, 386, 485, 773, 779, 1221, 1319
BmiI	GGNNCC	4 cut(s)	99, 667, 1071, 1321
BmrFI	CCNGG	5 cut(s)	489, 777, 878, 1179, 1225
BmsI	GCATC	7 cut(s)	412, 636, 667, 745, 895, 1011, 1337
BpiI	GAAGAC	1 cut(s)	1303
Bpu10I	CCTNAGC	1 cut(s)	447
BpuEI	CTTGAG	1 cut(s)	34
BpuMI	CCSGG	1 cut(s)	878
Bsa29I	ATCGAT	2 cut(s)	395, 720
BsaAI	YACGTR	1 cut(s)	253
BsaBI	GATNNNNATC	1 cut(s)	426
BsaI	GGTCTC	1 cut(s)	155
BsaJI	CCNNGG	4 cut(s)	93, 488, 595, 1323
BsaWI	WCCGGW	1 cut(s)	617
Bsc4I	CCNNNNNNNGG	3 cut(s)	482, 601, 883
Bse118I	RCCGGY	1 cut(s)	668
Bse1I	ACTGG	3 cut(s)	799, 820, 1153
Bse21I	CCTNAGG	1 cut(s)	783
Bse3DI	GCAATG	2 cut(s)	181, 834
Bse8I	GATNNNNATC	1 cut(s)	426
BseBI	CCWGG	4 cut(s)	489, 777, 1179, 1225
BseCI	ATCGAT	2 cut(s)	395, 720
BseDI	CCNNGG	4 cut(s)	93, 488, 595, 1323
BseGI	GGATG	5 cut(s)	682, 738, 793, 886, 895
BseJI	GATNNNNATC	1 cut(s)	426
BseLI	CCNNNNNNNGG	3 cut(s)	482, 601, 883
BseMI	GCAATG	2 cut(s)	181, 834
BseMII	CTCAG	2 cut(s)	438, 633
BseNI	ACTGG	3 cut(s)	799, 820, 1153
BseRI	GAGGAG	2 cut(s)	899, 1031
BseXI	GCAGC	1 cut(s)	625
BseYI	CCCAGC	1 cut(s)	672
Bsh1285I	CGRYCG	1 cut(s)	195
BshFI	GGCC	7 cut(s)	379, 387, 798, 851, 1115, 1177, 1210
BshVI	ATCGAT	2 cut(s)	395, 720
BsiEI	CGRYCG	1 cut(s)	195
BsiHKAI	GWGCWC	3 cut(s)	228, 924, 963
BsiHKCI	CYCGRG	1 cut(s)	1322
BsiSI	CCGG	5 cut(s)	116, 229, 618, 669, 877
BslFI	GGGAC	2 cut(s)	625, 1305
BslI	CCNNNNNNNGG	3 cut(s)	482, 601, 883
BsmAI	GTCTC	1 cut(s)	155
BsmFI	GGGAC	2 cut(s)	625, 1305
BsmI	GAATGC	1 cut(s)	640
BsnI	GGCC	7 cut(s)	379, 387, 798, 851, 1115, 1177, 1210
Bso31I	GGTCTC	1 cut(s)	155
BsoBI	CYCGRG	1 cut(s)	1322
Bsp1286I	GDGCHC	3 cut(s)	228, 924, 963
Bsp1407I	TGTACA	1 cut(s)	1278
Bsp143I	GATC	8 cut(s)	38, 53, 73, 192, 235, 427, 717, 1069
BspACI	CCGC	6 cut(s)	45, 171, 221, 389, 609, 1126
BspANI	GGCC	7 cut(s)	379, 387, 798, 851, 1115, 1177, 1210
BspCNI	CTCAG	2 cut(s)	439, 634
BspDI	ATCGAT	2 cut(s)	395, 720
BspHI	TCATGA	1 cut(s)	547
BspLI	GGNNCC	4 cut(s)	99, 667, 1071, 1321
BspMI	ACCTGC	3 cut(s)	7, 734, 832
BspPI	GGATC	6 cut(s)	46, 68, 230, 725, 1064, 1077
BspTNI	GGTCTC	1 cut(s)	155
BsrBI	CCGCTC	1 cut(s)	609
BsrDI	GCAATG	2 cut(s)	181, 834
BsrFI	RCCGGY	1 cut(s)	668
BsrGI	TGTACA	1 cut(s)	1278
BsrI	ACTGG	3 cut(s)	799, 820, 1153
BssAI	RCCGGY	1 cut(s)	668
BssECI	CCNNGG	4 cut(s)	93, 488, 595, 1323
BssMI	GATC	8 cut(s)	38, 53, 73, 192, 235, 427, 717, 1069
BssSI	CACGAG	1 cut(s)	805
Bst2BI	CACGAG	1 cut(s)	805
Bst2UI	CCWGG	4 cut(s)	489, 777, 1179, 1225
Bst4CI	ACNGT	2 cut(s)	837, 1278
Bst6I	CTCTTC	1 cut(s)	968
BstAPI	GCANNNNNTGC	1 cut(s)	1334
BstAUI	TGTACA	1 cut(s)	1278
BstBAI	YACGTR	1 cut(s)	253
BstC8I	GCNNGC	4 cut(s)	389, 453, 607, 1175
BstDEI	CTNAG	4 cut(s)	447, 526, 642, 783
BstDSI	CCRYGG	1 cut(s)	595
BstF5I	GGATG	5 cut(s)	682, 738, 793, 886, 895
BstHHI	GCGC	1 cut(s)	258
BstKTI	GATC	8 cut(s)	41, 56, 76, 195, 238, 430, 720, 1072
BstMAI	GTCTC	1 cut(s)	155
BstMBI	GATC	8 cut(s)	38, 53, 73, 192, 235, 427, 717, 1069
BstMCI	CGRYCG	1 cut(s)	195
BstMWI	GCNNNNNNNGC	2 cut(s)	606, 1334
BstNI	CCWGG	4 cut(s)	489, 777, 1179, 1225
BstSCI	CCNGG	5 cut(s)	487, 775, 876, 1177, 1223
BstSFI	CTRYAG	1 cut(s)	511
BstV1I	GCAGC	1 cut(s)	625
BstV2I	GAAGAC	1 cut(s)	1303
BstX2I	RGATCY	2 cut(s)	38, 1069
BstYI	RGATCY	2 cut(s)	38, 1069
Bsu15I	ATCGAT	2 cut(s)	395, 720
Bsu36I	CCTNAGG	1 cut(s)	783
BsuRI	GGCC	7 cut(s)	379, 387, 798, 851, 1115, 1177, 1210
BsuTUI	ATCGAT	2 cut(s)	395, 720
BtgI	CCRYGG	1 cut(s)	595
BtrI	CACGTC	1 cut(s)	409
BtsCI	GGATG	5 cut(s)	682, 738, 793, 886, 895
BtsI	GCAGTG	1 cut(s)	852
BtsIMutI	CAGTG	3 cut(s)	852, 1146, 1274
BveI	ACCTGC	3 cut(s)	7, 734, 832
Cac8I	GCNNGC	4 cut(s)	389, 453, 607, 1175
CaiI	CAGNNNCTG	1 cut(s)	686
CciI	TCATGA	1 cut(s)	547
CfoI	GCGC	1 cut(s)	258
Cfr10I	RCCGGY	1 cut(s)	668
Cfr13I	GGNCC	9 cut(s)	63, 182, 378, 386, 485, 773, 779, 1221, 1319
ClaI	ATCGAT	2 cut(s)	395, 720
Csp6I	GTAC	4 cut(s)	541, 578, 802, 1279
CspCI	CAANNNNNGTGG	2 cut(s)	848, 883
CviAII	CATG	6 cut(s)	296, 431, 548, 987, 1054, 1294
CviQI	GTAC	4 cut(s)	541, 578, 802, 1279
DdeI	CTNAG	4 cut(s)	447, 526, 642, 783
DpnI	GATC	8 cut(s)	40, 55, 75, 194, 237, 429, 719, 1071
DpnII	GATC	8 cut(s)	38, 53, 73, 192, 235, 427, 717, 1069
EaeI	YGGCCR	1 cut(s)	796
Eam1104I	CTCTTC	1 cut(s)	968
EarI	CTCTTC	1 cut(s)	968
Eco31I	GGTCTC	1 cut(s)	155
Eco32I	GATATC	1 cut(s)	928
Eco47I	GGWCC	7 cut(s)	63, 182, 485, 773, 779, 1221, 1319
Eco57I	CTGAAG	1 cut(s)	491
Eco81I	CCTNAGG	1 cut(s)	783
Eco88I	CYCGRG	1 cut(s)	1322
EcoO109I	RGGNCCY	4 cut(s)	378, 485, 773, 1221
EcoRI	GAATTC	1 cut(s)	289
EcoRII	CCWGG	4 cut(s)	487, 775, 1177, 1223
EcoRV	GATATC	1 cut(s)	928
FaeI	CATG	6 cut(s)	299, 434, 551, 990, 1057, 1297
FalI	AAGNNNNNCTT	2 cut(s)	965, 997
FaqI	GGGAC	2 cut(s)	625, 1305
FatI	CATG	6 cut(s)	295, 430, 547, 986, 1053, 1293
FauI	CCCGC	2 cut(s)	396, 602
FauNDI	CATATG	1 cut(s)	934
FbaI	TGATCA	1 cut(s)	427
FblI	GTMKAC	1 cut(s)	198
Fnu4HI	GCNGC	3 cut(s)	46, 172, 639
FokI	GGATG	5 cut(s)	689, 745, 800, 893, 902
Fsp4HI	GCNGC	3 cut(s)	46, 172, 639
FspBI	CTAG	1 cut(s)	480
FspI	TGCGCA	1 cut(s)	257
GlaI	GCGC	1 cut(s)	257
GluI	GCNGC	3 cut(s)	46, 172, 639
GsaI	CCCAGC	1 cut(s)	676
HaeIII	GGCC	7 cut(s)	379, 387, 798, 851, 1115, 1177, 1210
HapII	CCGG	5 cut(s)	116, 229, 618, 669, 877
HhaI	GCGC	1 cut(s)	258
Hin1II	CATG	6 cut(s)	299, 434, 551, 990, 1057, 1297
Hin6I	GCGC	1 cut(s)	256
HinP1I	GCGC	1 cut(s)	256
HindIII	AAGCTT	1 cut(s)	460
HinfI	GANTC	5 cut(s)	442, 500, 551, 683, 1091
HpaII	CCGG	5 cut(s)	116, 229, 618, 669, 877
HphI	GGTGA	2 cut(s)	347, 848
Hpy166II	GTNNAC	3 cut(s)	199, 250, 804
Hpy188I	TCNGA	5 cut(s)	23, 94, 471, 505, 563
Hpy188III	TCNNGA	6 cut(s)	51, 101, 446, 548, 1285, 1322
Hpy8I	GTNNAC	3 cut(s)	199, 250, 804
Hpy99I	CGWCG	4 cut(s)	61, 190, 193, 410
HpyAV	CCTTC	4 cut(s)	391, 769, 1281, 1314
HpyCH4III	ACNGT	2 cut(s)	837, 1278
HpyCH4IV	ACGT	3 cut(s)	252, 408, 704
HpyCH4V	TGCA	7 cut(s)	145, 649, 680, 827, 845, 1024, 1105
HpyF10VI	GCNNNNNNNGC	2 cut(s)	606, 1334
HpyF3I	CTNAG	4 cut(s)	447, 526, 642, 783
HpySE526I	ACGT	3 cut(s)	252, 408, 704
Hsp92II	CATG	6 cut(s)	299, 434, 551, 990, 1057, 1297
HspAI	GCGC	1 cut(s)	256
Ksp22I	TGATCA	1 cut(s)	427
Kzo9I	GATC	8 cut(s)	38, 53, 73, 192, 235, 427, 717, 1069
LmnI	GCTCC	1 cut(s)	231
Lsp1109I	GCAGC	1 cut(s)	625
LweI	GCATC	7 cut(s)	412, 636, 667, 745, 895, 1011, 1337
MaeI	CTAG	1 cut(s)	480
MaeII	ACGT	3 cut(s)	252, 408, 704
MaeIII	GTNAC	3 cut(s)	705, 811, 1057
MalI	GATC	8 cut(s)	40, 55, 75, 194, 237, 429, 719, 1071
MbiI	CCGCTC	1 cut(s)	609
MboI	GATC	8 cut(s)	38, 53, 73, 192, 235, 427, 717, 1069
MboII	GAAGA	4 cut(s)	16, 340, 985, 1303
MfeI	CAATTG	1 cut(s)	317
MflI	RGATCY	2 cut(s)	38, 1069
MhlI	GDGCHC	3 cut(s)	228, 924, 963
MluCI	AATT	5 cut(s)	24, 289, 317, 871, 1252
MlyI	GAGTC	1 cut(s)	692
MmeI	TCCRAC	1 cut(s)	186
MseI	TTAA	3 cut(s)	522, 584, 1080
MslI	CAYNNNNRTG	2 cut(s)	435, 966
MspI	CCGG	5 cut(s)	116, 229, 618, 669, 877
MspR9I	CCNGG	5 cut(s)	489, 777, 878, 1179, 1225
MunI	CAATTG	1 cut(s)	317
Mva1269I	GAATGC	1 cut(s)	640
MvaI	CCWGG	4 cut(s)	489, 777, 1179, 1225
MwoI	GCNNNNNNNGC	2 cut(s)	606, 1334
NciI	CCSGG	1 cut(s)	878
NdeI	CATATG	1 cut(s)	934
NdeII	GATC	8 cut(s)	38, 53, 73, 192, 235, 427, 717, 1069
NlaIII	CATG	6 cut(s)	299, 434, 551, 990, 1057, 1297
NlaIV	GGNNCC	4 cut(s)	99, 667, 1071, 1321
NmuCI	GTSAC	1 cut(s)	705
NsbI	TGCGCA	1 cut(s)	257
PagI	TCATGA	1 cut(s)	547
PaqCI	CACCTGC	1 cut(s)	832
PctI	GAATGC	1 cut(s)	640
PfeI	GAWTC	4 cut(s)	442, 500, 551, 1091
PkrI	GCNGC	3 cut(s)	47, 173, 640
Ple19I	CGATCG	1 cut(s)	195
PleI	GAGTC	1 cut(s)	691
PpsI	GAGTC	1 cut(s)	691
Ppu21I	YACGTR	1 cut(s)	253
PpuMI	RGGWCCY	3 cut(s)	485, 773, 1221
Psp5II	RGGWCCY	3 cut(s)	485, 773, 1221
Psp6I	CCWGG	4 cut(s)	487, 775, 1177, 1223
PspFI	CCCAGC	1 cut(s)	672
PspGI	CCWGG	4 cut(s)	487, 775, 1177, 1223
PspN4I	GGNNCC	4 cut(s)	99, 667, 1071, 1321
PspPI	GGNCC	9 cut(s)	63, 182, 378, 386, 485, 773, 779, 1221, 1319
PspPPI	RGGWCCY	3 cut(s)	485, 773, 1221
PstNI	CAGNNNCTG	1 cut(s)	686
PsuI	RGATCY	2 cut(s)	38, 1069
PvuI	CGATCG	1 cut(s)	195
RsaI	GTAC	4 cut(s)	542, 579, 803, 1280
RsaNI	GTAC	4 cut(s)	541, 578, 802, 1279
RseI	CAYNNNNRTG	2 cut(s)	435, 966
SaqAI	TTAA	3 cut(s)	522, 584, 1080
SatI	GCNGC	3 cut(s)	46, 172, 639
Sau3AI	GATC	8 cut(s)	38, 53, 73, 192, 235, 427, 717, 1069
Sau96I	GGNCC	9 cut(s)	63, 182, 378, 386, 485, 773, 779, 1221, 1319
SchI	GAGTC	1 cut(s)	692
ScrFI	CCNGG	5 cut(s)	489, 777, 878, 1179, 1225
SduI	GDGCHC	3 cut(s)	228, 924, 963
SfaNI	GCATC	7 cut(s)	412, 636, 667, 745, 895, 1011, 1337
SfcI	CTRYAG	1 cut(s)	511
SinI	GGWCC	7 cut(s)	63, 182, 485, 773, 779, 1221, 1319
SmiMI	CAYNNNNRTG	2 cut(s)	435, 966
SmlI	CTYRAG	1 cut(s)	49
SmoI	CTYRAG	1 cut(s)	49
Sse9I	AATT	5 cut(s)	24, 289, 317, 871, 1252
SsiI	CCGC	6 cut(s)	45, 171, 221, 389, 609, 1126
SspMI	CTAG	1 cut(s)	480
StyD4I	CCNGG	5 cut(s)	487, 775, 876, 1177, 1223
TaaI	ACNGT	2 cut(s)	837, 1278
TaiI	ACGT	3 cut(s)	255, 411, 707
TaqI	TCGA	8 cut(s)	56, 72, 191, 395, 405, 720, 753, 1357
TasI	AATT	5 cut(s)	24, 289, 317, 871, 1252
TatI	WGTACW	3 cut(s)	540, 801, 1278
TauI	GCSGC	2 cut(s)	48, 174
TfiI	GAWTC	4 cut(s)	442, 500, 551, 1091
Tru1I	TTAA	3 cut(s)	522, 584, 1080
Tru9I	TTAA	3 cut(s)	522, 584, 1080
TscAI	CASTG	3 cut(s)	852, 1153, 1281
TseFI	GTSAC	1 cut(s)	705
TseI	GCWGC	1 cut(s)	638
Tsp45I	GTSAC	1 cut(s)	705
TspDTI	ATGAA	5 cut(s)	17, 302, 536, 1173, 1310
TspGWI	ACGGA	1 cut(s)	174
TspRI	CASTG	3 cut(s)	852, 1153, 1281
VpaK11BI	GGWCC	7 cut(s)	63, 182, 485, 773, 779, 1221, 1319
XapI	RAATTY	1 cut(s)	289
XmiI	GTMKAC	1 cut(s)	198
XspI	CTAG	1 cut(s)	480

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.